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Microscopically and Molecular Detection of Theileria Species in Sheep in Baghdad Province, Iraq 伊拉克巴格达省绵羊中伊勒菌的显微及分子检测
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.2
A. Faraj, Dhirar Assi
The study was conducted to estimate the prevalence of Theileriosis in sheep in Baghdad city, as well as study the effect of age, sex, and months in the infection rate by examination of 180 of blood samples collected during the period from the beginning of October 2020 to the end of March 2021. All samples were examined by microscopic examination, and 100 blood samples collected randomly from total 180 were molecular detection by using conventional PCR. The total infection rate of the microscopic infection was 33.33%(60/180). The result showed a significant difference (P<0.05) between two different ages of animals that infected with Theileria spp and the adult sheep(>1y), which recorded the higher 50% (50/100), while the lower infection rate 12.5% (10/80) was among the young sheep(6mns-1y). concerning sex and months, there was no significant difference between female and male ,also among study months. The conventional PCR technique was used in the molecular study; 70(70%) out of 100 blood samples were selected randomly from 180 sheep. PCR products of teen were selected randomly for DNA analysis to obtain the partial nucleotides sequence of 18S ribosomal RNA gene . The PCR product was processed as a wave-like shape at (1104bp) after that sequences were recorded in NCBI with No.1 (MW735685.1), No.2(MW735686.1), No.3 (MW735687.1), No.5 (MW735689.1),No.7 (MW735691.1),No.8 (MW735692.1), No.10 (MW735694.1) for Theileria ovis and No.1 No.4 (MW735688.1), No.6 (MW735690.1), No.9 (MW735693.1) for Theileria
该研究旨在通过对2020年10月初至2021年3月底收集的180份血液样本进行检查,估计巴格达市绵羊中希勒菌病的流行情况,并研究年龄、性别和月份对感染率的影响。所有样本均进行镜检,180份血样中随机抽取100份,采用常规PCR方法进行分子检测。镜检感染总感染率为33.33%(60/180)。结果显示差异显著(P1y),较高的50%(50/100),而幼羊(6mn -1y)感染率较低(12.5%(10/80))。在性别和月份方面,男女之间没有显著差异,在研究月份之间也没有显著差异。分子研究采用常规PCR技术;从180只羊中随机抽取100份血液样本70份(70%)。随机选取青少年的PCR产物进行DNA分析,获得18S核糖体RNA基因的部分核苷酸序列。在NCBI中分别记录1号(MW735685.1)、2号(MW735686.1)、3号(MW735687.1)、5号(MW735689.1)、7号序列,将PCR产物在1104bp处处理成波浪状(MW735691.1),八号(MW735692.1)、10号(MW735694.1)和1号(MW735688.1)、6号(MW735690.1)、9号(MW735693.1)
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引用次数: 0
Protective Effects of Vitamin D3 on Male Rat’s Hepatic Cell Injured by Diclofenac, A Histopathological Study 维生素D3对双氯芬酸损伤公鼠肝细胞保护作用的组织病理学研究
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.10
Ali A. Tala’a
This experiment aimed to study the protective effects of vitamin D3 on hepatic cells injured by Diclofenac. Twenty-one male rats, 8 to 12 weeks of age and in weight from 180 to 220 grams, were housed in a standard, pathogen-free environment in the “College of Veterinary Medicine, University of Fallujah,” given unrestricted access to standard rat water and food. Then the rats were divided into 3 groups (each group containing 7 rats): G1 control group (IM injection of distill water for 7 days), G2 Diclofenac only (7-day course of a 10 mg/kg IM injection of diclofenac), G3 received intramuscular injections of VD3 three times per week at a dose of 1000 IU/Kg, then a 7-day course of a 10 mg/kg IM injection of diclofenac. Liver histological sections of the normal control (G1) showed no histopathological changes, while the histopathological sections of the liver of G2 exhibit hydropic degeneration, blood vessels congestion and inflammatory cell infiltration, Liver sections of G3 presented blood vessels congestion and mild inflammatory cell infiltration. This study concluded that VD3 can decrease the liver damage caused by diclofenac
本实验旨在研究维生素D3对双氯芬酸损伤肝细胞的保护作用。21只雄性大鼠,8至12周龄,体重180至220克,被安置在费卢杰大学兽医学院的标准无病原体环境中,可以不受限制地获得标准的老鼠水和食物。然后将大鼠分为3组(每组7只大鼠):G1对照组(蒸馏水IM注射7天),G2只注射双氯芬酸(10 mg/kg IM注射双氯芬酸7天疗程),G3每周肌肉注射3次VD3,剂量为1000 IU/kg,然后注射10 mg/kg IM双氯芬酸7天疗程。正常对照组(G1)的肝脏组织学切片没有组织病理学变化,而G2的肝脏组织病理学切片表现为水肿变性、血管充血和炎症细胞浸润,G3的肝脏切片表现为血管充血和轻度炎症细胞浸润。本研究得出结论,VD3可以减少双氯芬酸引起的肝损伤
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引用次数: 0
Synergistic Effect of Raw Tribulus terrestris With Vitamin E on Some Biochemical and Physiological Parameters in local Male Rabbits 雷公藤与维生素E对家兔某些生化生理指标的协同作用
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.5
Hamzah R. Kh., AL-Musawi Q.
This study was designed to investigate the synergistic effect of adding Tribulus terrestris with vitamin E to the feeding of domestic male rabbits on some biochemical and physiological traits. Where the experiment was conducted in the animal house at the Faculty of Veterinary Medicine. University of Baghdad, for 60 days from January until the end of February 2022. The experiment was carried out using 28 domestic rabbits 3 to 4 months of age, were divided into four groups, all animals were fed the same traditional feed mixture – the first control group T1, the 2nd group T2 received (Tribulus terrestris) , 3rd group T3 received Tribulus terrestris with vitamin E and 4th group T4 only received vitamin E. The dose of Tribulus terrestris was 1 g /animal /daily while the dose of vitamin E was 60 mg/ animal/daily for 8 weeks. The results are shown the addition Tribulus terrestris with or without vitamin E increased significantly (P<0.05) in hemoglobin levels also hematocrit while non-significant differences in leukocytes, erythrocytes count and glucose concentration, while the total protein, albumin and globulin increased significantly (P<0.05) in rabbits as compared with the control group. From results concluded that the Tribulus terrestris plant and vitamin E have a positive effect on some blood traits and serum proteins.
本试验旨在研究在家兔饲粮中添加蒺藜和维生素E对家兔某些生化生理性状的增效作用。实验是在兽医学院的动物屋进行的。巴格达大学,为期60天,从2022年1月到2月底。实验进行了使用国内28个兔子3到4个月大的时候,被分为四个组,所有的动物喂养相同的传统饲料的混合物——第一组T1, T2收到(旁通),第二组第三组T3收到刺蒺藜,维生素E和4组T4只收到了维生素E .刺蒺藜的剂量是1 g /动物/每日的剂量维生素E是60毫克/动物/每天8周。结果表明:与对照组相比,添加或不添加维生素E的蒺藜组血红蛋白水平和红细胞压积显著升高(P<0.05),白细胞、红细胞计数和葡萄糖浓度差异不显著(P<0.05),总蛋白、白蛋白和球蛋白显著升高(P<0.05)。结果表明,蒺藜植物和维生素E对一些血液性状和血清蛋白有积极的影响。
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引用次数: 0
Histopathological Changes in Lungs and Tracheas of Broiler Chickens Infected With Infectious Bronchitis Virus 鸡传染性支气管炎病毒感染后肺和气管的组织病理学变化
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.1.7
Saja S. Abbood, B. Ali
Infectious bronchitis is an acute extremely infectious respiratory illness caused by the avian gamma-corona virus. Infection with the infectious bronchitis virus predisposes the bird to secondary bacterial infection, worsening the situation. The infection causes severe morbidity and variable mortality in broilers, as well as a significant decrease in the layer production of eggs. This study was conducted to evaluate the pathological changes that occur in natural infection with the infectious bronchitis virus. Samples were collected from clinical cases submitted for necropsy at local veterinary clinics in Anbar province, Iraq. Tissues for molecular detection included tracheas, lungs, and kidneys. Samples confirmed infection by PCR used in the histopathological study. Histopathological sections from samples that tested positive showed variable lesions from one farm to another. In some cases, there was mild heterophilic infiltration and some times accompanied by deciliation and sometimes accompanied by subepithelial edema. In more severe cases there was congestion of blood vessels along heterophilic infiltration along with desquamation of the epithelial layers. The histopathological section in a lung of infected birds showed inflammatory cells infiltration consisting mainly of heterophils in the interstitial tissue. In some samples, there was extensive hemorrhage filling the atrial lumen and infiltration of eosinophils and heterophils along with interstitial hemorrhage. From this study, we concluded that viruses circulating in Iraq causes more pulmonary lesions when compared to lesions in the trachea.
传染性支气管炎是由禽伽玛冠状病毒引起的一种急性极传染性呼吸道疾病。感染传染性支气管炎病毒使禽类容易继发细菌感染,使情况恶化。这种感染在肉鸡中引起严重的发病率和可变死亡率,并显著降低产蛋量。本研究旨在评价自然感染传染性支气管炎病毒后发生的病理变化。样本是从伊拉克安巴尔省当地兽医诊所提交尸检的临床病例中收集的。用于分子检测的组织包括气管、肺和肾脏。经PCR证实感染的样本用于组织病理学研究。阳性样本的组织病理学切片显示不同农场的不同病变。在一些病例中,有轻微的嗜异性浸润,有时伴有脱落,有时伴有上皮下水肿。在更严重的情况下,血管充血沿嗜异性浸润伴上皮层脱屑。感染鸟类肺组织病理切片显示间质组织中以嗜白细胞为主的炎性细胞浸润。在一些样本中,心房腔内大量出血,嗜酸性粒细胞和嗜嗜性粒细胞浸润,并伴有间质出血。从这项研究中,我们得出结论,与气管病变相比,在伊拉克传播的病毒引起的肺部病变更多。
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引用次数: 0
Review about Sarcocystis in Ruminants in Iraq 伊拉克反刍动物肉孢子虫研究进展
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.1
Howaida Abed, A. Fadhil
This review was conducted togive an overview of the current state of knowledge on Sarcocystis infectivity, pathogenicity, and transmissibility here, based on our current understanding virulence. Sarcocystis species are parasitic protozoa. Sarcocystis is about 220 species that may be found all over the world. Heteroxen and protozoan parasites Sarcocystis spp. develop cysts in intermediate hosts’ tissues and are thrown out as sporocysts with definitive hosts. Herbivores, which are their intermediate hosts, have cysts in their hearts, oesophagus, diaphragms, tongues, jaws, and other skeletal muscles. In general, canid-transmitted Sarcocystis spp. are more pathogenic than felid-transmitted Sarcocystis spp. Fever, anemia, weight loss, decreased wool quality and milk output, and abortion are all signs of intermediate hosts.
这篇综述是为了在我们目前对毒力的理解的基础上,概述目前关于Sarcocystis传染性、致病性和传播性的知识现状。Sarcocystis是一种寄生原生动物。Sarcocystis大约有220种,可能在世界各地都有发现。杂种和原生动物寄生虫Sarcocystis spp.在中间宿主的组织中形成包囊,并作为孢子囊与最终宿主一起被抛出。草食动物是它们的中间宿主,它们的心脏、食道、横膈膜、舌头、颌骨和其他骨骼肌中都有囊肿。一般来说,犬科动物传播的Sarcocystis spp.比猫传播的Sarcocystis sp.更具致病性。发烧、贫血、体重减轻、羊毛质量和产奶量下降以及流产都是中间宿主的迹象。
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引用次数: 0
Detection of Blood Parasites in Different Pet Birds in Erbil City 埃尔比勒市不同宠物鸟类血液寄生虫的检测
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.4
Waad Khalaf
The current study was conducted to find out the different species of blood parasites that infect pet birds (Melopsittacus undulatus, Carduelis chloris and Carduelis carduelis) and to know the infection rates with these parasites and their relationship with the sex of the bird, by examining (62) blood samples collected from three species of different pet birds in the Erbil city, of both sexes, during October 2019 to July 2021. The current study showed that pet birds were infected with Haemosporidian parasites with a total percentage of 35.48%, and the highest percentage was in the Eurasian goldfinch (Carduelis carduelis) with 41.17%, followed by the greenfinch (Carduelis chloris) with 31.25%, and the lowest rate was in the budgerigar (Melopsittacus undulatus) by 25%. Also, the study showed that the infection rate of Plasmodium spp. and Haemoproteus spp. was 77.27%, and 45.45% respectively. It was noted that there were no significant differences in the infection rates with blood parasites between males and females. While significant differences were recorded in the infection rates with species of blood parasites according to different types of infection, as the single infection recorded the highest infection rate of 77.27%, while the double infection was lower with a rate of 22.72%.
目前的研究是通过检查(62)从埃尔比勒市三种不同的宠物鸟(两性)采集的血液样本来找出感染宠物鸟的不同种类的血液寄生虫(波纹卷尾藻、氯化卡杜利斯和卡杜利斯),并了解这些寄生虫的感染率及其与鸟类性别的关系,2019年10月至2021年7月。目前的研究表明,宠物鸟感染血孢子虫的总比例为35.48%,欧亚金翅雀感染率最高,为41.17%,其次是绿翅雀,为31.25%,虎皮鹦鹉感染率最低,为25%。研究还表明,疟原虫和哈氏杆菌的感染率分别为77.27%和45.45%。值得注意的是,男性和女性的血液寄生虫感染率没有显著差异。不同感染类型的血液寄生虫感染率存在显著差异,单次感染感染率最高,为77.27%,而双重感染感染率较低,为22.72%。
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引用次数: 0
Seroprevelance and Antibiotic Resistance of Salmonella spp. Isolated From Pigeons and Their Environment in Nineveh Governorate 尼尼微省鸽子及其环境中分离的沙门氏菌血清阳性率和耐药性
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.6
M. J. Mohammed, A. M. Shareef
This study was conducted to explore the antibody titers and antibiotic sensitivity of salmonella organisms isolated from pigeons and their surrounding in Nineveh governorate during the period from November 2021 to March 2022. Thirty out of 83 positive Salmonella spp. isolated from 400 pigeons and 150 samples of their environment in a previous study (unpublished data) were subjected to an antibiogram sensitivity test. Nearly half of the isolates (n=16) were tested for ß- lactamase production. Seroprevalence of 92 blood samples collected from the same pigeons (69/adults and 23/squabs) were used for the estimation of S. enteritides antibody titers. The antibiogram profile of tested Salmonella spp. revealed high resistance (73.33%) to Ciprofloxacin followed by Colistin (70%), Florphenicol (66.6%), Sulphamethoxazole-Trimethoprim (60%), Lincomycin and Amoxicillin (46.6), Spiramycin and Doxycycline (40%), Tetracycline (36.66%), Levofloxacin and Enrofloxacin (33.33%), Phosphomycin (26.6%), Norfloxacin (23.3%), Cephalexin, Neomycin and Gentamycin 16.66%. Beta-lactmase producing Salmonella spp. revealed that 3 isolates out of 13(18.75%) were positive by acidimetric, 7/16(43.75%) using extended spectrum ß -lactmase and 6/16(37.5%) in extended spectrum ß -lactmase type ampC methods. Antibody titers to S. enteritides naturally exposed pigeons obtained by Indirect ELISA revealed a significant (P≤0.ooo1) difference between adults and squabs mean titers. In conclusion, pigeons are considered one of the Salmonella spp. sources that may aid in the cross-contamination of Salmonella spp. from pigeons to the surroundings, humans or animals.
本研究旨在研究2021年11月至2022年3月期间尼尼微省鸽子及其周围分离的沙门氏菌的抗体滴度和抗生素敏感性。在先前的一项研究(未发表的数据)中,从400只鸽子和150个环境样本中分离出83种阳性沙门氏菌,其中30种接受了抗生素谱敏感性测试。近一半的分离株(n=16)进行了ß-内酰胺酶生产测试。采用92份相同样本(69/成年鸽和23/乳鸽)的血清阳性率测定肠炎沙门氏菌抗体滴度。沙门氏菌对环丙沙星的耐药程度最高(73.33%),其次为粘菌素(70%)、氟霉素(66.6%)、磺胺甲氧嘧啶-甲氧苄啶(60%)、林可霉素-阿莫西林(46.6%)、螺旋霉素-多西环素(40%)、四环素(36.66%)、左氧氟沙星-恩诺沙星(33.33%)、磷霉素(26.6%)、诺氟沙星(23.3%)、头孢氨苄、新霉素和庆大霉素(16.66%)。产β -内酰胺酶沙门氏菌13株中有3株(18.75%)经酸化法检测阳性,7株(43.75%)经增谱β -内酰胺酶检测阳性,6株(37.5%)经增谱β -内酰胺酶型ampC检测阳性。间接酶联免疫吸附法测定的自然暴露鸽子的抗体滴度与乳鸽平均滴度有显著差异(P≤0.0001)。总之,鸽子被认为是沙门氏菌的来源之一,可能有助于沙门氏菌从鸽子到周围环境、人类或动物的交叉污染。
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引用次数: 0
Some Physiological and Serum Biochemical Reference Values of Adult Chukar Partridge (Alectoris chukar Kurdestanica) in Kurdistan Region-Iraq 伊拉克库尔德斯坦地区成年丘卡Partridge(Alectoris Chukar Kurdestanica)的某些生理和血清生化参考值
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.9
O. Pavel, B. Abbas, H. Dyary
The physiological parameters and blood biochemical parameters of wild Chukar partridges in the Sulaymaniyah Governorate of the Kurdistan Region-Iraq were measured in this study. During this research, 27 healthy Alectoris chukar partridges (9 males, 18 females), 8–12 months, were captured in the mountains of Penjwin district in the east of Sulaimani City from March to July 2022. Physiological parameters, including weight, heart rate (HR), respiratory rate (RR), and cloacal temperature (CT), were recorded for each partridge. Blood samples were collected from the jugular or wing vein to determine the serum biochemical parameters such as alanine transaminase levels, aspartate transaminase, blood urea nitrogen, creatinine, gamma-glutamyl transferase, total proteins, albumin, globulin, glucose, and total bilirubin. The males were significantly larger in weight (498.7 g) than the females (446.5 g). However, no significant differences were observed in the physiological parameters’ values between the sexes. Also, the serum biochemical parameters showed no sex-related differences between the sexes, except that the alanine transaminase level was significantly higher in the males (p < 0.05). The birds were released to their natural habitat after the studies were conducted. As a pioneer work, these physiological and serum biochemistry values may serve as a reference range of Chukar partridge in diagnostic procedures for both physiological and pathological conditions.
本研究测定了伊拉克库尔德斯坦地区苏莱曼尼亚省野生丘卡鹧鸪的生理参数和血液生化参数。在这项研究中,2022年3月至7月,在苏莱曼尼市东部Penjwin区的山区捕获了27只健康的Alectoris chukar鹧鸪(9只雄性,18只雌性),年龄8-12个月。记录每只鹧鸪的生理参数,包括体重、心率(HR)、呼吸频率(RR)和泄殖腔温度(CT)。从颈静脉或翼静脉采集血样,以测定血清生化参数,如丙氨酸转氨酶水平、天冬氨酸转氨酶、血尿素氮、肌酸酐、γ-谷氨酰转移酶、总蛋白、白蛋白、球蛋白、葡萄糖和总胆红素。雄性的体重(498.7克)明显大于雌性(446.5克)。然而,性别之间的生理参数值没有观察到显著差异。此外,血清生化参数显示,除了雄性的丙氨酸转氨酶水平显著较高外,两性之间没有性别相关差异(p<0.05)。研究完成后,这些鸟被放归了它们的自然栖息地。作为一项开创性的工作,这些生理和血清生物化学值可以作为丘卡鹧鸪在生理和病理条件诊断程序中的参考范围。
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引用次数: 0
Histological and Histochemical Study of Pyloric Region in Abomasum of Adult Local Buffalos (Bubalus Bubalis) 成年本地水牛(Bubalus Bubalis)胃幽门区的组织学和组织化学研究
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.3
Nazeha Ahmed, Thekta Saleh, Ahmed Aljawad
The current study was conducted to demonstrate the histological feature of the pyloric region of the abomasum in local buffalos and to focus on the distribution of different types of carbohydrates in the pylorus wall, where digestion has a great impact on animal production and economical outcome. Six samples of abomasum were collected from adult healthy local buffalos from the Mosul abattoir. then the pylorus region samples were sectioned from the pylorus, histologically processed, and stained with hematoxylin & eosin stain, Masson’s trichrome, periodic acid Schiff and alcian blue. The histological findings of the pyloric region revealed that the mucosal wall of pylorus has depressions called gastric pits, these pits occupied the most of lamina propria receiving the mucus secretion of pyloric glands, which appeared short, tortuous, tubular in the anterior part of the pylorus, and became longer, straight in the middle part of the pylorus. The submucosa is characterized by the presence of lymphatic nodules. The histochemical findings revealed the presence of neutral and acidic (mixed) glycoprotein in the pyloric region, where acidic glycoprotein was found in terminal pyloric glands, while neutral glycoprotein was present mainly in gastric pits. The mid gland of the anterior part is distinguished by neutral glycoprotein, whereas the acidic glycoprotein was evident in the mid gland of the middle part of the pylorus. The surface epithelium is characterized by the presence of both neutral and acidic types of glycoprotein. Furthermore, glycogen is present in both gastric pits in the caudal part and terminal pyloric glands of the anterior part. Glycosaminoglycans were predominant at the terminal part of pyloric glands and present in little amounts in the middle part and absent in gastric pits. In conclusion presence of acidic glycoprotein in pyloric glands confirm the presence of mucus neck cells due to their acidic secretion, while the presence of neutral glycoprotein mainly in gastric pits indicates the formation of the mucosal gastric barrier.
本研究旨在证明本地水牛胃幽门区的组织学特征,并重点研究不同类型碳水化合物在幽门壁中的分布,消化对动物生产和经济效益有很大影响。从摩苏尔屠宰场的成年健康当地水牛身上采集了六份皱胃样本。然后从幽门切片,组织学处理,并用苏木精-伊红染色、Masson三色染色、碘酸Schiff和阿尔西安蓝染色。幽门区的组织学检查结果显示,幽门粘膜壁有称为胃凹坑的凹陷,这些凹坑占据了接受幽门腺粘液分泌的固有层的大部分,幽门腺粘液在幽门前部呈短、弯曲、管状,在幽门中部变长、直。黏膜下层的特征是存在淋巴结。组织化学结果显示,幽门区存在中性和酸性(混合)糖蛋白,其中酸性糖蛋白存在于幽门末端腺体,而中性糖蛋白主要存在于胃凹陷。幽门前部的中腺以中性糖蛋白为特征,而酸性糖蛋白在幽门中部的中腺明显。表面上皮的特征是存在中性和酸性类型的糖蛋白。此外,糖原存在于尾部的胃小窝和前部的幽门末端腺中。氨基甘聚糖主要存在于幽门腺的末端部分,少量存在于中间部分,不存在于胃凹坑中。总之,幽门腺中酸性糖蛋白的存在证实了粘液颈细胞的存在,因为它们的酸性分泌,而中性糖蛋白的存在主要表明胃粘膜屏障的形成。
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引用次数: 0
Essential of Hygienic Practices on Bacterial Contamination in some Restaurants of Al- Karkh Area, Baghdad, Iraq 伊拉克巴格达Al-Karkh地区一些餐馆细菌污染的卫生措施要点
Pub Date : 2022-12-15 DOI: 10.37940/ajvs.2022.15.2.8
M. Al-Obaidi, T. Dawood
This study aimed to evaluate good manufacturing practices in food safety of ten different restaurants in the Al-Karkh area of Baghdad, Iraq. Forty samples collected from were collected from knives, food cutting boards, tables, hands and nails workers in restaurants. In addition. 70 food handlers were selected. Through structured interviews, information on the checklist for Good Manufacturing Practices in Food Safety, Food handlers’ general checklist for good hygiene, and Personal Hygiene Checklist were collected. The overall viable bacterial count before Good Hygiene Practices was significantly higher (P<0.05) than the total bacterial counts after Good Hygiene Practices. The highest viable bacterial counts before Good Hygiene Practices were recorded by food cutting boards (4.03±0.20) log10 cfu/cm2, while (3.90±0.23, 3.51±0.18, 3.00±0.18) log10 cfu/cm2 from tables, hands and nails of workers and knives respectively. Although the results showed a non-significant difference in the viable bacterial counts after Good Hygiene Practices from 10 restaurants collected from the AL-Karkh area. The percentages were (100%) after the training and lecture about Good Hygiene Practices to all checklist points except special basins for washing hands was (90%). However, the results of the General Information of food handlers of Good Hygiene checklist and Personal Hygiene Checklist showed that there were significant differences (P≤0.05) between the self-reported attitudes of food handlers before the one-week training and after Good Hygiene Practices.
本研究旨在评估伊拉克巴格达Al-Karkh地区十家不同餐馆在食品安全方面的良好生产实践。从餐馆的刀具、食品砧板、桌子、手和指甲工人身上采集了40个样本。此外选择了70名食品管理员。通过结构化访谈,收集了食品安全良好生产规范检查表、食品经营者良好卫生通用检查表和个人卫生检查表的信息。良好卫生措施实施前的总活菌数显著高于良好卫生措施后的总菌数(P<0.05)。《良好卫生规范》之前,食品砧板记录的最高活菌数为(4.03±0.20)log10 cfu/cm2,而桌子、工人的手和指甲以及刀具的活菌数分别为(3.90±0.23、3.51±0.18、3.00±0.18)log10 cfu/cm2。尽管从AL Karkh地区收集的10家餐馆的良好卫生规范后,结果显示活菌计数没有显著差异。在关于良好卫生习惯的培训和讲座之后,除洗手专用盆外,所有检查表点的百分比为(100%),为(90%)。然而,良好卫生检查表和个人卫生检查表的食品处理人员一般信息结果显示,食品处理人员在一周培训前和良好卫生实践后的自我报告态度存在显著差异(P≤0.05)。
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