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The Efficacy of Mesenchymal Stem Cells loaded in Acacia Gum Hydrogel and Acacia Gum Hydrogel on Skin Wounds Healing in Rats 金合欢胶水凝胶和金合欢胶水凝胶负载间充质干细胞对大鼠皮肤伤口愈合的影响
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2021.15.1.8
Wissam Abdullah Alhayani
Cutaneous wound healing processes start at the beginning of the trauma leading to impairment of physical and functional tissue continuity. The present work aimed to clarify the efficacy of stem cells loaded in acacia gum hydrogel and acacia gum hydrogel on skin wound healing in rats. Thirty-six adult male rats (Wister rats). The weight of the rats was estimated between 150-160 grams and the animals were divided into three groups (n=12). In Group A (control group), the surgical skin wound was established and treated with normal saline only. Group B: surgical skin wound was made and covered with Acacia gum hydrogel. Group C: surgical skin wound was made and covered with mesenchymal stem cells loaded in Acacia gum hydrogel. Examination of wound healing on days 3, 7, 14 and 21 for gross and microscopic pathological changes. The gross pathological evaluation showed no significant difference between all groups but the histopathological result showed the Acacia gum improved wound recovery better than the control group, although the wound was not completely closed on day 21 in Acacia gum and control groups contrary to the stem cells treated group, which showed complete wound closure. The best ever histopathological result of wounds healing in mesenchymal stem cells loaded in acacia gum hydrogel treated groups.
皮肤伤口愈合过程始于创伤开始,导致身体和功能组织连续性受损。本工作旨在阐明装载在阿拉伯胶水凝胶和阿拉伯胶水凝胶中的干细胞对大鼠皮肤伤口愈合的功效。36只成年雄性大鼠(Wister大鼠)。大鼠的体重估计在150-160克之间,动物被分为三组(n=12)。在A组(对照组)中,建立外科皮肤伤口并仅用生理盐水治疗。B组:制作外科皮肤伤口,用Acacia树胶水凝胶覆盖。C组:制作外科皮肤伤口,用Acacia胶水凝胶负载的间充质干细胞覆盖。在第3天、第7天、第14天和第21天检查伤口愈合的大体和微观病理变化。大体病理学评估显示,所有组之间没有显著差异,但组织病理学结果显示,Acacia胶比对照组更好地改善了伤口恢复,尽管Acacia胶和对照组在第21天伤口没有完全闭合,而干细胞治疗组显示伤口完全闭合。在阿拉伯胶水凝胶处理组中装载的间充质干细胞中伤口愈合的最佳组织病理学结果。
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引用次数: 0
Effect of Raw and Boiled Oak Quercus brantii in Performance, Biochemical and Blood Indices, and Proximate Composition of Cyprinus Carpio L. 生橡和熟橡对鲤鱼生产性能、生化指标、血液指标及主要成分的影响。
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2022.15.1.1
N. M. Abdulrahman, N. Hassan, H. Ameen, H. Sabri, V. M. Ahmad, K. O. Qader, B. Hassan, Hevar Aras Hama-Salih, Hemin Nuraddin Mohammed
This study was conducted in fish diseases lab. / College of veterinary medicine/ university of Sulaimani to evaluate the effect of boiling oak as compared with raw oak on the performance of common carp at 0.05 significant level for all studied parameters. This study was carried out on C. carpio L. for five treatments/ three replicates. The experimental trial consists of five treatments with three duplicates, each containing seven fish: Group 1: Diet without any supplement as a control group, Group 2: adding 10g raw oak/ kg diet, Group 3: adding 20g raw oak/kg diet, Group 4: adding 10g boiled oak/kg diet, Group 5: 20 grams of boiling oak/kg diet. The second group with 10g raw oak/ kg diet and T5 with 20g boiled oak/ kg diet was high significantly in the final weight, weight gain, significant and relative growth rate. T3 with 20g raw oak/ kg diet was higher significantly in FCR, T4 with 10g boiled oak/kg diet was higher significantly in FER. RBC *1012/L was higher in T4 with 10g boiled oak/ kg diet and T5 with 20g boiled oak/ kg diet were higher significantly, in HGB g/L the T2 with 10g raw oak/ kg diet and T3 with 20g raw oak/kg diet were higher significantly. T3 with 20g raw oak/kg diet was significant in each of the indices MCH pg and MCHC g/L. T4 with 10g boiled oak/kg diet was higher significantly in each of HCT %. The control was significantly higher in PLT *109/L. T5 with 20g boiled oak/ kg diet was significantly high in Sugar and HDL. Total Protein was significantly high in control and T2 with a 10g raw oak/ kg diet. Cholesterol was significantly high in T2, T4, and T5 as compared to the control group. GOT and CKI was significant in T3. A Hepatosomatic index was higher significantly in each of T2, T3 and T5. Gill somatic Index was significant in all treatments as compared to T3. Gonadosomatic Index was significant in all treatments as compared to T2 and T4, in the Intestine Weight Index and Intestine Length Index, all treatments were significantly different from the control. In the Intestine Length (Fish Length) Index, T2 was substantially greater. The current study’s findings imply that oak acorn powder is a viable feed additive, indicating a starting point for additional research into its potential use as an antioxidant and immune booster in various fish species.
这项研究是在鱼类疾病实验室/兽医学院/苏莱曼尼大学进行的,目的是在所有研究参数的0.05显著水平下,评估煮沸橡木与生橡木对鲤鱼性能的影响。本研究在C.carpio L.上进行了五次处理/三次重复。实验试验由五个处理组成,三个重复,每个处理包含七条鱼:第一组:不添加任何补充剂的饮食作为对照组,第二组:添加10g生橡木/kg饮食,第三组:添加20g生橡木/kg膳食,第四组:添加10g煮橡木/kg饮食、第五组:添加20 g煮橡木g/kg饮食。第二组采用10g生橡木/kg日粮,T5采用20g煮橡木/kg日料,其最终体重、增重、显著生长率和相对生长率均显著高于对照组。在FCR中,20g生橡木/kg日粮的T3显著升高,10g煮橡木/kg日料的T4显著升高。RBC*1012/L在T4组和T5组中均显著升高,在HGB/L组中,T2组和T3组均显著升高。T3与20g生橡木/kg日粮的MCH pg和MCHC g/L各指标均显著相关。10克水煮橡木/kg日粮中T4在各HCT%中均显著升高。PLT*109/L的对照组显著高于对照组。T5和20g煮橡木/kg日粮的糖和高密度脂蛋白显著较高。总蛋白在对照组和T2和10g生橡木/kg日料中显著较高。与对照组相比,T2、T4和T5的胆固醇显著升高。GOT和CKI在T3有显著性差异。肝体细胞指数在T2、T3和T5中均显著升高。与T3相比,Gill体细胞指数在所有处理中都是显著的。与T2和T4相比,所有处理的性腺体细胞指数均显著,在肠道重量指数和肠道长度指数方面,所有处理均与对照组显著不同。在肠长度(鱼的长度)指数中,T2明显更大。目前的研究结果表明,橡果粉是一种可行的饲料添加剂,这为进一步研究其在各种鱼类中作为抗氧化剂和免疫增强剂的潜在用途提供了一个起点。
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引用次数: 0
Effectiveness of Bovine Tunica Vaginalis Powder in the Prevention of Tendon Adherence Following Tendon Repair Process in Bucks 牛Tunica阴道粉预防雄鹿肌腱修复过程中肌腱粘连的效果
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2022.15.1.3
A. Khudair, Hassanain A. Emran
The current study was designed to investigate the efficacy of lyophilized bovine tunica vaginalis powder on peritendinous adhesion of induced defect in superficial digital flexor tendon (SDFT) in goats. The study was carried out on 18 adult bucks, the surgical procedure was done under highly aseptic conditions. Prior to surgery, animals were sedated by intramuscular injection of 2 % xylazine in a dose 0.5 mg/kg body weight, then the site of the operation is anesthetized by linear infiltration of 2% lidocaine hydrochloride. The surgical incision is made on the skin on the palmer surface of the metacarpal bone of the left forelimb to expose the (SDFT), a (0.1 depth, 0.2 width, and l Long) cm defect is made on the exposed tendon, this gap is filled with drops of sterile normal saline in (9) animals, and mentioned as a control group, while the gap of the rest (9) animals is filled with the lyophilized powder as treated group. Subcutaneous tissue was closed using absorbable suture material by simple continuous suture technique, and a simple interrupted suture mattress using non-absorbable suture material for closing the skin. The macroscopic findings exhibited a significant difference in adhesion scores between the 2nd, and 3rd periods of assessment post-operation in the first and second groups, with no significant differences between groups. The conclusion of this study is the tunica vaginalis scaffold has no obvious effect on decreasing adhesion between a tendon and surrounding tissues.
本研究旨在研究冻干牛阴道膜粉对山羊指浅屈肌腱(SDFT)诱导性缺损腱周粘连的影响。这项研究是在18只成年雄鹿身上进行的,手术是在高度无菌的条件下进行的。手术前,通过肌肉注射剂量为0.5 mg/kg体重的2%甲苯噻嗪使动物镇静,然后通过2%盐酸利多卡因的线性渗透麻醉手术部位。在左前肢掌骨掌表面的皮肤上进行手术切口,以暴露(SDFT),在暴露的肌腱上形成(0.1深,0.2宽,1长)cm的缺损,在(9)只动物中用无菌生理盐水滴填充该间隙,并作为对照组提及,而其余(9)只动物的间隙被作为处理组的冻干粉填充。皮下组织采用可吸收缝合材料通过简单连续缝合技术闭合,并采用非吸收缝合材料的简单间断缝合垫闭合皮肤。宏观检查结果显示,第一组和第二组在术后第二和第三个评估期的粘附得分存在显著差异,各组之间没有显著差异。本研究的结论是,鞘膜支架对减少肌腱与周围组织之间的粘附没有明显作用。
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引用次数: 0
Clinical and Histological Aspects of Cryptorchidism in Dogs and Cats 狗和猫隐睾的临床和组织学研究
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2022.15.1.5
O. Ali, T. Ali, G. M. Raouf, O. Dana
This study was aimed to investigate the incidence of cryptorchidism among dogs and tomcats were undergoing elective neutering. Cryptorchidism is diagnosed through clinical examination, palpation of the scrotum, and the ultrasonic finding of the retained testicles within the inguinal or the abdomen. Eleven cases (11.22%) of cryptorchid were identified from a total of 98 cases (42 dogs and 56 tomcats) that underwent elective castration. It was found that different breeds of dogs and tomcats were affected, including Maltese dog, Anatolian shepherd dog, Terrier dogs, Persian cat, Turkish angora cat, and the Domestic Shorthair cat. The locations of cryptorchid testis were varied from inguinal (3.06%) to intra-abdominal (8.16%) origin with either unilateral (10.2%) or bilateral (1.02%) of retained testis and their ratio was 3:3. The histopathological findings of the cryptorchid testes were showed a decrease and disorganization of the seminiferous tubules with severe hypo-spermatogenesis in both species, dogs, and tomcats. In addition, there was also a typical Sertoli cell-only syndrome (SCOS), which was characterized by larger nuclei and atrophy of many tubules, which were completely replaced by hyaline substances. In conclusion, the occurrence of cryptorchidism in Anatolian Shepherd dogs and the Domestic Shorthair cats were first documented in the literature. Although, no significant differences were found in the incidence of cryptorchidism in both species.
本研究旨在调查正在接受选择性绝育手术的狗和猫的隐睾发生率。隐睾是通过临床检查、阴囊触诊和腹股沟或腹部保留睾丸的超声检查来诊断的。在总共98例(42只狗和56只猫)接受选择性阉割的病例中,发现了11例(11.22%)隐睾。研究发现,不同品种的狗和猫都受到了影响,包括马耳他犬、安纳托利亚牧羊犬、梗犬、波斯猫、土耳其安哥拉猫和家养短毛猫。隐睾的位置从腹股沟(3.06%)到腹腔内(8.16%)不等,单侧(10.2%)或双侧(1.02%)保留睾丸,其比例为3:3。隐睾睾丸的组织病理学结果显示,在这两个物种,狗和猫中,曲精管减少和紊乱,精子生成严重不足。此外,还有一种典型的仅支持细胞综合征(SCOS),其特征是细胞核变大,许多小管萎缩,这些小管完全被透明质物质所取代。总之,文献中首次记录了安纳托利亚牧羊犬和家养短毛猫发生隐睾的情况。尽管如此,两个物种的隐睾发病率没有发现显著差异。
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引用次数: 0
Identification and Characterization of Canine Mammary Tumors Stem Cells: A Review 犬乳腺肿瘤干细胞的鉴定与表征
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2021.15.1.9
E. Al-Hamdany
Glands cancers consider the most types of cancers in males or females or both. Many challenges are present in controlling cancers in dogs, the first one is related to populations of tumour cells define as cancer stem cells, cancer cells are resistant to chemotherapy and have the ability to metastasise and returned after successful treatment. Cancer consists of different cells that contain a subtype of cell with stemness characteristics, like high tumourigenicity, the self-renew ability to differentiate and resistance to chemo and radiotherapy. These cells are termed cancer stem cells (CSCs) and are involved in the initiation of tumours, recurrence and metastasis. Canine mammary cell tumours are malignant neoplasms characterized by metastasis and a high infiltration rate. Canine mammary tumours (CMT) occur in female dogs frequently, six to seven (6-7) years old animals ages are more susceptible to the disease, however, it can be diagnosed at 9–11 years of age, which is known as the cancer age, it has the ability to form tumours spheres which are considered a characteristic of stem cells. it is difficult to clarify the histological finding base on Hematoxyline and Eosin stains policy, so biomarkers are recommended like CD44+and CD24- ,also Immunohistochemistry . These tumours are composed of mesenchymal cells andor myoepithelial cells with proliferative luminal epithelial cells, cartilage, and osseous tissues. Annually around the world, between 200-250 mammary gland tumours out of 100.000 female dogs, 50% were malignant. Surgical removal is the treatment of choice, however, it is ineffective because of the metastasis high rate. It is important to focus on the analysis of CSCs high incidences in dogs (bitches) and CSCs isolation and diagnosis.
腺癌被认为是男性或女性或两者中最常见的癌症类型。在控制狗的癌症方面存在许多挑战,第一个挑战与被定义为癌症干细胞的肿瘤细胞群体有关,癌症细胞对化疗具有耐药性,并且有能力转移并在成功治疗后返回。癌症由不同的细胞组成,这些细胞含有一种具有干性特征的细胞亚型,如高肿瘤原性、自我分化能力以及对化疗和放疗的抵抗力。这些细胞被称为癌症干细胞(CSCs),参与肿瘤的发生、复发和转移。犬乳腺细胞瘤是一种以转移和高浸润率为特征的恶性肿瘤。犬乳腺肿瘤(CMT)经常发生在母狗身上,六到七(6-7)岁的动物更容易感染这种疾病,然而,它可以在9-11岁时被诊断出来,也就是所谓的癌症年龄,它有能力形成肿瘤球体,这被认为是干细胞的特征。基于苏木精和Eosin染色策略很难阐明组织学发现,因此推荐生物标志物,如CD44+和CD24-,以及免疫组织化学。这些肿瘤由间充质细胞和/或肌上皮细胞与增殖性管腔上皮细胞、软骨和骨组织组成。每年在世界各地,100000只母狗中有200-250只乳腺肿瘤,其中50%是恶性的。手术切除是首选的治疗方法,但由于转移率高,手术切除无效。重点分析犬(母犬)CSCs的高发病率以及CSCs的分离和诊断是很重要的。
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引用次数: 0
Effect of Zinc Oxide Nanoparticles Synthesis by ( Zingiber Officinale) Ginger Extract in Some Microbiological Quality of Fresh Chicken Meat During Referginated Storage 姜提取物合成氧化锌纳米颗粒对冷藏保鲜鸡肉微生物品质的影响
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2022.15.1.2
A. Saeed, M. T. Abdulwahid
This study, include the green synthesis of ZnO nanoparticles using ethanol extract of (Zingiber officinale) ginger was investigated in order to evaluate its antibacterial effect on fresh chicken meat. Determining the microbiological quality of meat involves performing various plate counts indicators include, including total bacterial count (TBC) and the total coliform counts TCC the UV-Visible Spectrophotometry and FTiR (Fourier Transform infrared spectroscopy) were used to characterize the GnZnO nanoparticles. A total of 180 samples of fresh chicken meat were subjected to (0.5, 1, and 1.5) mg/ml of GnZnONPs and bacteriologically examined was done. significant absorption of UV region at peak370nm verified the presence of ZnO NPs. The FT-IR spectra recorded the existence of biomolecules responsible for the reduction and capping of the green synthesized, Also observed result of a meat sample treated with GnZnONPs showed a significant (p < 0.05) reduction in (TBC) through 2, 5, and 7 days of storage at 4°C (1.5 mg). While the highest count recorded in control (untreated samples) reached 11.360.04 CFU.g-1 at the end of the experiment, the TCC also decreased significantly (p< 0.05) after 5,7 days of storage at 40 C when the meat samples were treated with GnZnONPs compared to the control sample. The findings show that treating poultry meat with GnZNoNPs extends the shelf-life of the meat, and maintains its quality throughout the storage period
本研究以生姜乙醇提取物为原料,研究绿色合成氧化锌纳米粒子对新鲜鸡肉的抑菌效果。测定肉类的微生物质量包括进行各种平板计数,指标包括细菌总数(TBC)和大肠菌群总数(TCC),紫外可见分光光度法和傅里叶变换红外光谱法(FTiR)对GnZnO纳米颗粒进行了表征。对180份新鲜鸡肉样品进行(0.5、1和1.5)mg/ml的GnZnONPs处理,并进行细菌学检测。370nm峰处紫外吸收显著,证实了ZnO纳米粒子的存在。FT-IR光谱记录了对合成的绿色进行还原和封盖的生物分子的存在,并且观察到GnZnONPs处理的肉类样品在4°C (1.5 mg)下保存2、5和7 d时(TBC)的显著(p < 0.05)降低。而对照组(未经处理的样本)最高计数为11.360.04 CFU。gznonps处理肉类样品在40℃下保存5、7 d后,TCC也较对照样品显著降低(p< 0.05)。研究结果表明,用GnZNoNPs处理禽肉可以延长肉的保质期,并在整个储存期间保持其质量
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引用次数: 0
Estimation of the Nitrite Concentration in the Raw Milk and Locally Produced Soft Cheese in Different Area of Baghdad, Iraq 伊拉克巴格达不同地区原料奶和土产软奶酪中亚硝酸盐浓度的测定
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2022.15.1.6
Mahdi. A. Al-Shuwaili, Z. Khudhir
Samples for both raw milk and locally produced soft cheese totally (150), 75 for each were collected from different regions of Baghdad from October 2020 to July 2021 to estimate nitrite concentration. Ion exchange chromatography was used to detect the nitrite levels in the samples. The Limit of Detection (LOD) and Limit of Quantification (LOQ) were 0.5 mg/L and 1.7 mg/L respectively. The levels of nitrite in the samples of raw milk were (0.5-1.5 mg/L) with average (0.9 ± 0.4 mg/L), while the concentrations were (0.8-2.3 g/kg) in the soft cheese samples with average (1.4 ± 0.4 g/kg). The nitrite level in the soft cheese was higher than in the raw milk according to the months significantly (P<0.05). The presence of nitrite in milk and cheese indicates a poorness of hygiene practices during milking and post-milking operations, as well as during cheese production.
2020年10月至2021年7月,从巴格达不同地区共采集了生牛奶和当地生产的软奶酪样本(150份),各75份,以估计亚硝酸盐浓度。采用离子交换色谱法检测样品中的亚硝酸盐含量。检测限(LOD)和定量限(LOQ)分别为0.5 mg/L和1.7 mg/L。生奶样品中的亚硝酸盐水平为(0.5-1.5 mg/L),平均值为(0.9±0.4 mg/L),而软奶酪样品中的浓度为(0.8-2.3 g/kg),平均为(1.4±0.4 g/kg)。软奶酪中的亚硝酸盐水平根据月份显著高于生奶(P<0.05)。牛奶和奶酪中亚硝酸盐的存在表明在挤奶和挤奶后操作以及奶酪生产过程中缺乏卫生习惯。
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引用次数: 0
Effect of Adding Lecithin to Diets on Productive Performance of Laying Hens 日粮中添加卵磷脂对蛋鸡生产性能的影响
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2021.15.1.7
Mustafa A. Mohsen, B. H. Mousa
This study was conducted in the poultry farm of the Department of Animal Production / College of the Agriculture / University of Anbar, during the period from 20/7/2021 to 15/10/2021, with the aim of studying the effect of adding lecithin to the diet on the productive performance of laying hens. 60 layer hens of the Lohmann Brown breed, aged 43 weeks were used in this experiment. The layer hens were distributed randomly into five treatments with four replicates per treatment (3 hens/repeat). Birds fed balanced diets contain 5 levels of lecithin as below: T1 (control groups ), T2, T3, T4 and T5 adding 0.25, 0.50, 0.75 and 1.0% of lecithin respectively to the basic diet. The results showed the superiority of the additional treatments in the productive traits during most of the production periods, As all the additional treatments during the most stages of production. All additive treatments showed superiority significantly during the third productive period, When calculating the general average, treatments T2 and T5 were significantly superior to the control treatment in the percentage of egg production (Hen Day Production%). Also, T5 showed superiority in the average egg weight during the second and third periods, and in the general average.T5 and T4 showed an increase in the egg mass-produced during the second period and three periods and with the general average. The results showed that there were significant differences between the different treatments in the rates of feed consumption during all stages of production. Also, the additive treatmentT5 showed the best feed conversion ratio during the second and third production periods and in the general average.
本研究于2021年7月20日至2021年10月15日在安巴尔大学农学院动物生产系家禽养殖场进行,旨在研究在日粮中添加卵磷脂对蛋鸡生产性能的影响。本试验选用60只43周龄的Lohmann-Brown蛋鸡。蛋鸡被随机分为五个处理,每个处理四个重复(3只母鸡/重复)。喂食平衡日粮的鸟类含有以下5个水平的卵磷脂:T1(对照组)、T2、T3、T4和T5在基础日粮中分别添加0.25%、0.50、0.75和1.0%的卵磷脂。结果表明,在生产的大部分时期,附加处理在生产性状上都具有优势,而在生产的大多数阶段,附加处理都具有优势。在第三个生产期,所有添加处理都表现出显著的优势。计算总平均值时,T2和T5处理的产蛋率(母鸡日产量%)显著优于对照处理。此外,T5在第二期和第三期的平均蛋重以及总平均蛋重方面表现出优势。T5和T4表现出在第二期和第三期大量生产的卵子的增加,并且与一般平均值一致。结果表明,在生产的各个阶段,不同处理的饲料消耗率存在显著差异。此外,添加剂处理T5在第二个和第三个生产期以及一般平均值中显示出最佳的饲料转化率。
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引用次数: 0
Diagnostic and Hematological Study in Sheep Infected With Gastrointestinal Nematode in Mosul City 摩苏尔市绵羊胃肠道线虫感染的诊断和血液学研究
Pub Date : 2022-06-01 DOI: 10.37940/ajvs.2022.15.1.4
D. Moosa, A. M. Hussien, Hajir M. Hameed, Saad A. Hasan
The aim of this study was to investigate the infection rates as well as the types of Gastrointestinal worms in local sheep admitted to the veterinary teaching hospital at the University of Mosul from the beginning of March 2021 until the end of August 2021, the study also included the effect of Gastrointestinal worms on blood picture in affected animals, 120 faeces and blood samples were collected from native sheep, 12 sheep were clinically normal and negative for gastrointestinal tract parasites and served as control, the results of laboratory examination of faeces samples showed 80 (120) samples were positive for Gastrointestinal worms with a total of infection rate 66.66%, Infection with eggs of Marshallagia spp represented the highest rate of infection amounting to 50 %, followed by infection with eggs of Ostertagia spp at a rate of 17.5 %, while the percentage of infection with eggs of Haemonchus spp. was 15%, Oesophagostomum spp. Nematodirus.spp, Trichostrongylus Spp had the lowest infection rates( 7.5,5.5) % respectively. Blood pictures of infected animals showed a significant decrease (P<0.05) in Red Blood Cell count (RBC), Pack Cell Volume (PCV), and Hemoglobin (Hb), with a significant increase (P<0.05) in Mean Corpuscular Volume (MCV), without significant changes in mean corpuscular haemoglobin concentration (MCHC) values, compared with a control group, while ESR values increased significantly in infected animals compared with control, the Result was showed a significant increased in Total leucocyte count with eosinophilia and lymphocytosis. These results showed the wide spread of Gastrointestinal worms infection in sheep in Mosul city, in addition to its effect on the blood picture.
本研究的目的是调查2021年3月初至2021年8月底在摩苏尔大学兽医教学医院收治的当地绵羊的感染率和胃肠道蠕虫的类型,研究还包括胃肠道蠕虫对受感染动物血液图像的影响,收集了120份当地绵羊的粪便和血液样本。12只羊临床正常,胃肠道寄生虫阴性,作为对照,实验室粪便标本检查结果显示,胃肠道寄生虫阳性80只(120只),总感染率66.66%,其中以Marshallagia虫卵感染率最高,达50%,其次为Ostertagia虫卵,感染率17.5%;血螨虫卵感染率为15%,食道口虫虫卵感染率为线虫病;其中,毛线虫感染率最低,分别为7.5%、5.5%。感染动物的血液图像显示,与对照组相比,感染动物的红细胞计数(RBC)、包细胞体积(PCV)和血红蛋白(Hb)显著降低(P<0.05),平均红细胞体积(MCV)显著升高(P<0.05),平均红细胞血红蛋白浓度(MCHC)值无显著变化,而感染动物的ESR值显著升高(P<0.05)。结果显示白细胞总数明显增加,伴嗜酸性粒细胞增多和淋巴细胞增多。这些结果表明,除了对血液图像的影响外,胃肠道蠕虫感染在摩苏尔市绵羊中广泛传播。
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引用次数: 0
Determination of the Toxic Dose of Experimental Lead Poisoning for Male Goats in Fallujah City, Iraq 伊拉克费卢杰市雄山羊实验性铅中毒毒性剂量的测定
Pub Date : 2021-12-30 DOI: 10.37940/ajvs.2021.14.2.7
O. Fahad, M. Al-Graibawi
This study aimed to induce lead poisoning experimentally in male goats to determine the toxic dose and investigate it is effects on hematological parameters, and the functions of the liver and kidney. The experiment was performed on 15 male goats, aged between 3 – 5 months with a mean weighing 13±0.65 kg. Goats were divided into five equal groups, the first represented the control group given tap water, while the other groups were given orally (by stomach tube) 70,100,200 and 400 mg/kg B.W. of lead acetate respectively, for 5 days. Blood was collected weekly for 4 weeks to estimate the concentrations of lead, hematological and biochemical analysis. The results indicated a significantly (P≤0.05) increase of lead(0.738±0.07ppm), only in goats which received 400 mg Pb/kg B.W of lead with symptoms included: depression, dullness, anemia, muscle twitching, staggering, and teeth grinding, with a significant reduction in erythrocyte count, packed cell volume, and hemoglobin 10.391±0.41*106/ml, 25.5±0.55%, and 8.30±0.19g/dl respectively, compared with the control ones. Also, increase in total white blood cell count to 9.098±0.08*103/ml, neutrophils 39.07±0.93%, monocytes 1.88±0.07%, and eosinophils 4.82±0.05%. The same group results showed significant elevations in the activities of liver enzymes; ALT 59.9±0.20u/l, AST 243±1.3u/l, in addition, the serum levels of creatinine and urea were also increased indicating renal frailer 1.96±0.05 mg/dl and 29.78±0.34 mg/dl respectively. In summary, this is the first study that proved the toxic dose of lead poisoning for male goats in Iraq and estimate their hazardous results on the hematological and chemobiological analyses on goats.
本试验旨在对公山羊进行实验性铅中毒试验,以确定中毒剂量,并探讨其对山羊血液学指标及肝肾功能的影响。试验选用15只3 ~ 5月龄、平均体重13±0.65 kg的公山羊。将山羊分为5组,对照组饲喂自来水,其余各组分别经胃管口服醋酸铅70,100,200和400 mg/kg B.W.,连续5 d。每周采集血液,连续4周评估铅浓度,进行血液学和生化分析。结果表明,与对照组相比,仅铅含量为400 mg Pb/kg B.W的山羊体内铅含量(0.738±0.07ppm)显著(P≤0.05)升高,表现为抑郁、迟钝、贫血、肌肉抽搐、蹒跚、磨牙,红细胞计数、堆积细胞体积、血红蛋白分别显著降低(10.391±0.41*106/ml、25.5±0.55%、8.30±0.19g/dl)。白细胞总数增加9.098±0.08*103/ml,中性粒细胞增加39.07±0.93%,单核细胞增加1.88±0.07%,嗜酸性粒细胞增加4.82±0.05%。同一组结果显示肝脏酶活性显著升高;ALT 59.9±0.20u/l, AST 243±1.3u/l,血清肌酐和尿素水平升高,提示肾衰分别为1.96±0.05 mg/dl和29.78±0.34 mg/dl。综上所述,这是第一次通过对山羊的血液学和化学生物学分析,证明了伊拉克公山羊铅中毒的毒性剂量,并估计了其危险结果。
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