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Effect of Monthly Variations on the Plasma Membrane, Acrosome and DNA Integrities of Spermatozoa in Friesian Holstein Bulls Born in Iraq 伊拉克出生的弗里斯-荷斯坦公牛精子膜、顶体和DNA完整性的月变化影响
Pub Date : 2020-12-16 DOI: 10.37940/ajvs.2020.13.2.11
Q. Aboud, S. Hatif
This study was aimed to evaluate the influence of months of the year on the quality of semen in Holstein bulls. A study carried out at artificial insemination centre/ Abou-Ghareeb/ western of Baghdad. A total of 160 ejaculates were collected from 15 bulls born in Iraq via the artificial vagina. The age of the bulls ranged between 4 to 5 years and the study period were December to March and September. The semen samples were diluted with Tris base extender. The semen was packed in a straw according to the program of artificial insemination centre. Semen characteristics (plasma membrane, acrosome, and DNA integrities) were evaluated. The results revealed a significant decrease (P≤0.01) in the plasma membrane and acrosome integrity in September as compared with December, January, February, and March. There was a significant decline (P≤0.05) in DNA integrity in September as compared with December, January, February, and March in fresh and frozen semen. In conclusion, the September month had a negative effect on the plasma membrane, acrosome, and DNA percentage in all bulls.
本研究旨在评估一年中几个月对荷斯坦公牛精液质量的影响。在巴格达西部阿布·加雷布人工授精中心进行的一项研究。通过人工阴道从伊拉克出生的15头公牛身上总共采集了160个精液。公牛的年龄在4至5岁之间,研究期为12月至3月和9月。精液样品用Tris碱增量剂稀释。精液按照人工授精中心的程序装在吸管里。评估精液特征(质膜、顶体和DNA完整性)。结果显示,与12月、1月、2月和3月相比,9月的质膜和顶体完整性显著降低(P≤0.01)。与12月、1月、2月和3月相比,9月新鲜精液和冷冻精液的DNA完整性显著下降(P≤0.05)。总之,九月对所有公牛的质膜、顶体和DNA百分比都有负面影响。
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引用次数: 0
Effect of Addition of Urea to The Barley Hay For Production of Gases In Vitro 大麦干草中添加尿素对体外产气的影响
Pub Date : 2019-12-01 DOI: 10.37940/ajvs.2019.12.2.4
The object of this study were determined the effect of adding urea (0,2%) and molasses (4%) to barley and straw on in vitro gas and methane production, in vitro fermentation characteristic (pH and ammonia nitrogen concentration) and in vitro digestibility, dry matter, organic matter and metabolizable energy, the samples incubated in 39oc water path for 24,48,72 and 96 h., the results indicated that there were significant decrease (p<0.01) in total gas and methane production after 48,72 and 96 h incubation periods in barley straw with 2% urea compared with control (without urea), while 24 h. total gas and methane production increased significant(p<0.01) in straw with 2% urea compared with control. However, metabolizable energy, organic matter digestibility, short chain fatty acids and metabolizable energy for lactation which determine by total gas production after 24 h. incubation increased significantly (p<0.01) in straw with 2% urea compared with no adding urea. The result showed no effects of treatments on pH in all incubation periods except 48h. were pH increased significantly (p<0.01) in compared with control straw with 2% urea .Ammonia nitrogen concentration increased significantly (p<0.01) in straw with 2% urea compared with control after 48h. of incubation period while control treatment increased significantly (p<0.01) after 96h. incubation period compared with straw with 2% urea in ammonia nitrogen concentration, no significant differences found in ammonia nitrogen concentration after 24 and 72 h. incubation period between treatments, moreover, the in vitro dry matter digestibility, organic matter digestibility and metabilizable energy increased significantly (p<0.01) in barley straw with 2% urea compared with control. The result indicated that adding 2% urea to barley straw increased in vitro gas and methane production which lead to increased in vitro organic and dry matter digestibility and metabolizable energy.
本研究的目的是测定大麦和秸秆中添加尿素(0.2%)和糖蜜(4%)对体外产气和甲烷、体外发酵特性(pH和氨氮浓度)和体外消化率、干物质、有机物和代谢能的影响,样品在39oc水通道中孵育24、48、72和96h。,结果表明,添加2%尿素的大麦秸秆在培养48、72和96h后,总产气量和甲烷量均显著低于对照组(p<0.01);添加2%尿素后24h,总产气和甲烷量显著高于对照组(p<0.01)。然而,与不添加尿素相比,添加2%尿素的秸秆的代谢能、有机物消化率、短链脂肪酸和泌乳代谢能(由24小时后的总产气量决定)显著增加(p<0.01)。结果表明,除48h外,各处理对pH值均无影响。与添加2%尿素的对照秸秆相比,pH值显著升高(p<0.01)。施用2%尿素的秸秆48h后氨氮浓度显著高于对照(p<0.01)。96小时后,对照组潜伏期显著延长(p<0.01)。与尿素浓度为2%的稻草相比,在培养24小时和72小时后,氨氮浓度没有显著差异。此外,与对照相比,尿素浓度为2%的大麦秸秆的体外干物质消化率、有机物消化率和代谢能显著提高(p<0.01)。结果表明,在大麦秆中添加2%尿素,可提高大麦秆的体外产气和甲烷量,提高有机物和干物质的体外消化率和代谢能。
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引用次数: 0
Isolation and Diagnosis of Pathogenic Bacteria from the Bowels of Local Chicken and Identifying Some of the Virulence Factors 地方鸡肠道致病菌的分离诊断及部分毒力因子的鉴定
Pub Date : 2019-12-01 DOI: 10.37940/ajvs.2019.12.2.5
Sumaya. Y. A. AL-Dabbagh
This study was conducted to the isolation and identification of bacteria from chicken intestine and livers in Mosul city. A total of 35 samples from intestine and 35 liver samples from local chicken were collected during a period from September 2018 to March 2019, the bacteria were diagnosed according to morphological, cultural and biochemical characteristics. The results showed (100%) positive to bacterial isolation for each samples of intestine and liver, (12) types of bacteria from (71) isolates for intestine, while (10) types from (36) isolates for liver. E. coli formed the highest percentage of intestinal isolates (28.16%), while Corynebacterium spp formed the highest percentage in liver isolates (33. 33%).The bacterial types were isolated from intestine included: E. coli (28.16%) Corynebacterium spp (25.35%), Enterococcus faecalis (15.49%), Klebsiella pneumonia (8.45%), Bacillus spp (7.04%), Proteus spp (5.63%), Staphylococcus aureus (4.22%), Lactobacillus spp (2.81%), Arcanobacterium pyogenes (1.4%), Citrobacter spp (1.4%). The bacterial types were isolated from liver included: Corynebacterium spp (33.33%), E.coli ( 19.44%), Staphylococcus aureus (16.6%), Bacillus spp (11.11%), Enterococcus faecalis (5.55%), Klebsiella pneumonia (5.55%), Pseudomonas aeruginosa (2.77%), Listeria monocytogenes (2.77%), Arcanobacterium pyogenes (2.77%). The bacterial types isolated from both intestine and liver were, E.coli, Corynebacterium spp, Staphylococcus aureus, Bacillus spp, Enterococcus faecalis ,Klebsiella pneumonia, Proteus spp and Arcanobacterium pyogenes. The virulence factors tests were used for some liver isolates which included, protease, licethinase, lipase, urease, coagulase and haemolysin.
本研究对摩苏尔市鸡小肠和肝脏中的细菌进行了分离鉴定。在2018年9月至2019年3月期间,共采集了35份来自当地鸡的肠道样本和35份肝脏样本,根据形态学、培养和生化特征对细菌进行了诊断。结果显示,肠道和肝脏的每个样本的细菌分离呈(100%)阳性,(71)个分离株的细菌类型呈(12)阳性,而(36)个分离物的细菌类型为(10)阳性。大肠杆菌在肠道分离株中的比例最高(28.16%),而棒状杆菌在肝脏分离株中比例最高(33.0%)。大肠杆菌(28.16%)棒状杆菌(25.35%)、粪肠球菌(15.49%)、肺炎克雷伯菌(8.45%)、芽孢杆菌(7.04%)、变形杆菌(5.63%)、金黄色葡萄球菌(4.22%)、乳酸杆菌(2.81%),柠檬酸杆菌属(1.4%)。从肝脏分离的细菌类型包括:棒状杆菌属(33.33%)、大肠杆菌属(19.44%)、金黄色葡萄球菌属(16.6%)、芽孢杆菌属(11.11%)、粪肠球菌属(5.55%)、肺炎克雷伯菌属(5.55%)、绿脓杆菌属(2.77%)、,化脓性Arcanobacterium pyogenes(2.77%)。从肠道和肝脏分离的细菌类型为:大肠杆菌、棒状杆菌属、金黄色葡萄球菌、芽孢杆菌属、粪肠球菌、肺炎克雷伯菌、变形杆菌属和化脓性Arconobacterium pyogenes。对部分肝分离株进行了毒力因子测定,包括蛋白酶、乙酰胆碱酯酶、脂肪酶、脲酶、凝固酶和溶血素。
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引用次数: 0
Survey for ovine and caprine brucellosis in Kirkuk 基尔库克绵羊和山羊布鲁氏菌病调查
Pub Date : 2019-06-30 DOI: 10.37940/AJVS.2019.12.1.1
Fatima Asgar
This study was conducted to determine the prevalence of brucellosis in the province of Kirkuk and its environs. Rose Bengal test was used on males and females of sheep and goats for the period from 1/1/2016 to 31/12/2016 in the veterinary hospital in Kirkuk and based on the cases received at the veterinary hospital. The incidence of brucellosis was 32.2% of total animals, 97.9% of females and 2.1% of males. For animal species, the rate of infection in sheep and goats was 89.6% and 10.4%, respectively. Abortion occurred in the eight, eleven and ninth month of the year and the most extreme in the Laylan, Yayci and City centre this percentage of the infection is high.
本研究旨在确定基尔库克省及其周边地区布鲁氏菌病的流行情况。2016年1月1日至2016年12月31日期间,在基尔库克的兽医医院,根据兽医医院收到的病例,对绵羊和山羊的雄性和雌性进行了玫瑰孟加拉测试。布鲁氏菌病的发病率为动物总数的32.2%,雌性为97.9%,雄性为2.1%。就动物种类而言,绵羊和山羊的感染率分别为89.6%和10.4%。堕胎发生在一年中的第八个月、第十一个月和第九个月,在Laylan、Yayci和市中心最为极端,感染率很高。
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引用次数: 0
Effect of Arginine and Selenium with Vitamin E on WBC and the level of hormones in Iraqi ewes discharged 精氨酸、硒和维生素E对伊拉克母羊出院后白细胞及激素水平的影响
Pub Date : 2019-06-30 DOI: 10.37940/AJVS.2019.12.1.2
I. Alawiy, T. Mohammed, A. Majeed
This study was achieved in a special farm in Basaer village which lies on the right side of the Euphrates in Heet district l Anbar, Heet lies 70 K.M west Ramadi during the pesiod, From 17/4/2018 to 30/5/2018. 28 ewes aged 2-4 years of awassi with average weight 40-50 k.g. and a one lambing. All ewes were diagnosed by using the real-time Ultrasonography to make sure there is no pregnancy among the female before the experiment. All ewes were synchronized with intravaginal sponges impregnated with 60 mg MAP for 12 days before sponges withdrawal and eCG 500 IU (Novormon made in Argentina). The pregnant ewes were divided randomly and equally (7 each group) Into 4 groups The ewes in the first group were received an intramuscular injection of 160 Mµ of arginine 3 times daily. The second group were treated with 2ml/head a combination of selenium plus vitamin E (vitamin E 68mg/ml Selenium 1.5mg/ml). The third group was treated by injection of 160 Mµ of arginine and selenium with vitamin E 2 ml/head. The four groups were injected with normal saline which was considered as a control group. The ewes were inseminated by ram at the end of the hormonal program for five days. The samples of blood were taken from the Jugular vein at four-day treatment Also, In the 4, 10, 15, 18. The results of the experiment showed no significant deferent in WBC, Progesterone estrogen, growth hormone, prolactin, early of pregnancy in the treatment with the control group. The experiment showed a significant difference in the Progesterone concentration within a single treatment in three treatment without control. concluded from this study that use of arginine and selenium with vitamin E improves ewes health, increasing the progesterone to maintain pregnancy.
这项研究是在Basaer村的一个特殊农场完成的,该农场位于Heet区l Anbar幼发拉底河右侧,Heet位于比西奥德时期拉马迪以西70公里处,2018年4月17日至2018年5月30日。年龄2-4岁的28只母羊,平均体重40-50公斤,一只产仔。实验前,所有母羊都通过实时超声检查进行了诊断,以确保母羊没有怀孕。将所有母羊与用60mg MAP浸渍的阴道内海绵同步12天,然后取出海绵和eCG 500IU(Novarmon,阿根廷制造)。怀孕母羊被随机等分(每组7只),分成4组。第一组母羊每天3次肌肉注射160Mµ精氨酸。第二组接受2ml/头硒加维生素E(维生素E 68mg/ml硒1.5mg/ml)的联合治疗。第三组接受160Mµ精氨酸和硒加维生素E2 ml/头的注射治疗。四组均注射生理盐水作为对照组。在激素程序结束后,母羊被公羊受精五天。在为期四天的治疗中,从颈静脉采集血液样本。此外,在第4、10、15、18天。实验结果表明,治疗组白细胞、孕激素、雌激素、生长激素、泌乳素、妊娠早期与对照组比较无显著差异。实验显示,在没有对照的三个处理中,单个处理中的孕酮浓度存在显著差异。本研究得出的结论是,精氨酸和硒与维生素E一起使用可以改善母羊的健康,增加孕酮以维持妊娠。
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引用次数: 3
Effect of the use of exogenous fibrolytic enzymes in blood parameters in local goats 外源性纤维分解酶对地方山羊血液参数的影响
Pub Date : 2019-06-20 DOI: 10.37940/AJVS.2019.12.1.7
Sawsan Al-Salmany, S. Khalaf, A. Taha
The study was carried out in the ruminant researches station / department of livestock research section / office of agricultural research in the district of Abu Ghraib / Baghdad to assess the effect of exogenous fibrolytic enzyme (EFE) (Safizym - France) in blood characters of local male kids. The experiment was conducted during the period from 21 May 2017 to 17 July 2017 (57) day. Eighteen males of domestic goats aged 4-5 months were used in this study. The animals were randomly assigned to three treatments (6 animals for each treatment) a control treatment was fed on basal ration without enzyme (Tc), a treated groups were supplemented with the EFE of 500 g / ton of concentrate feed (T1) and a treatment with the EFE of 1000 g / ton of concentrate feed (T2). The results showed no significant effect of the adding with EFE in the total protein, albumin and globulin, while there was an effect of the period of the weeks in the T1, where the superiority (P≤0.05) of the eighth week. The results showed a significant differences (P≤0.05) in the serum urea concentration. The second treatment decreased during the second and sixth weeks compared to the T1 and the control Tc. There was an effect of the period of the weeks within the same treatment, where all means in all treatments were reduced with progress the time of the experiment. The results showed no significant effect of the adding with EFE in both glucose and cholesterol. The means were within the normal range and the triglycerides were not affected but there were differences between the weeks within the treatment. Concentrations of HDL, LDL and VLDL were not affected by the addition of EFE, but significant differences (P≤0.05) were observed between the weeks within the treatments. The results showed that T2 treatment was superior during the zero time in the concentration of the ALP enzyme for T1 and Tc, and significant differences between the weeks within the treatments. T1 treatment (P≤0.05) exceeded T2 and Tc in the concentration of the ALT enzyme during the zero time and the fourth and sixth weeks with no effect for the period of the weeks within the same treatment. The treatment and period of the weeks did not affect the concentration of AST enzyme in the serum.
本研究在巴格达阿布格莱布区反刍动物研究站/畜牧研究部/农业研究办公室进行,以评估外源性纤维分解酶(EFE)(Safizym-France)对当地男性儿童血液特征的影响。实验在2017年5月21日至2017年7月17日期间进行(57)天。本研究使用了18只4-5个月大的公山羊。将动物随机分配到三个处理(每个处理6只动物),对照处理以不含酶的基础日粮(Tc)喂养,处理组补充500克/吨浓缩饲料的EFE(T1),处理组用1000克/吨精饲料的EFF(T2)。结果表明,添加EFE对总蛋白、白蛋白和球蛋白无显著影响,但对T1的周数有影响,其中第8周的优势(P≤0.05)。结果显示,血清尿素浓度有显著性差异(P≤0.05)。与T1和对照Tc相比,第二次治疗在第二周和第六周减少。在同一次治疗中出现了周数的影响,所有治疗中的所有手段都随着实验时间的推移而减少。结果表明,添加EFE对葡萄糖和胆固醇均无显著影响。平均值在正常范围内,甘油三酯没有受到影响,但在治疗的几周内存在差异。高密度脂蛋白、低密度脂蛋白和极低密度脂素的浓度不受添加EFE的影响,但在治疗的几周之间观察到显著差异(P≤0.05)。结果表明,在T1和Tc的ALP酶浓度为零时,T2治疗效果更好,治疗周间差异显著。T1治疗(P≤0.05)在第0周、第4周和第6周ALT酶浓度超过T2和Tc,在相同治疗的几周内没有效果。治疗和几周的时间不影响血清中AST酶的浓度。
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引用次数: 0
Effect Of Arginine On The Some Blood And Biochemical Parameters For Local Goat Females. 精氨酸对当地母羊某些血液和生化指标的影响。
Pub Date : 2019-06-20 DOI: 10.37940/ajvs.2019.12.1.4
A. Ahmed, T. Mohammed
This study was conducted in one of the private sector fields in Anbar province - Hit city - Albasaer village (70 km west of the Ramadi city,), for the period from 28/6/2018 to 1/9/2018. Twenty one local female goats aged between 2-4 years and weighing between 24.5 to 36.5 kg were used, Which have previous one birth or more. All female goats were tested using ultrasound to make sure they were not pregnant before the experiment began. Females were randomly divided into three equal groups (7 goats in each group).Vaginal sponges (60 mg MAP) were injected into the three groups at the same time. The first group T1 was injected intramuscularly with the amino acid, arginine (US Nevada manufacturing) in the muscles at 200μmol.kg Five days before the sponge was pulled out until the 17 day after the sponge was pulled out, While the second group T2 was injected with amino acid (arginine) at 160 μmol.kg. Five days before the sponge was pulled until the 17th day after that. The third group T3 control group was injected with 5 ml Normal Saline intramuscularly of the animal. All animals were injected three times daily from the eighth day after the sponge was placed (five days before removing of the sponge) until 17 days after the removing of sponge. Where the total number of injection days was 22 days. Blood samples were taken from the jugular vein before injection of the arginine on day 7 and day 12 of the sponge placed either after the sponge pulled the blood samples were taken on the days 2, 3, 4, 6, 9, 13, 18, respectively of the experiment After sponges removing. The objective of the study was to measure changes in the blood, biochemical parameters, during arginine treatment. The results of this study showed significant differences. T1 and T2 group were superior compared to control group in blood properties which include PCV in periods 2, 5, the number of white blood cells in periods 2, 3, 4, 5, 6, 9, MID in periods 3, 9 and Lymphocytes in periods 4, 5, 6, 9, While the neutrophil cells the period of 3. In terms of biochemical properties, the results showed a significant differences, between the treatments of T1 and T2 were superior compared to control treatments in the total protein concentration in period 6, and the globulin in period 6. We conclude that the use of different doses of arginine can improve the health status of female goats.
本研究于2018年6月28日至2018年9月1日在安巴尔省的一个私营部门油田——Hit市——Albasaer村(拉马迪市以西70公里)进行。使用了21只年龄在2-4岁之间、体重在24.5至36.5公斤之间的当地母山羊,这些山羊以前有过一次或多次分娩。实验开始前,所有母山羊都接受了超声波检测,以确保它们没有怀孕。雌性被随机分为三组(每组7只山羊)。将阴道海绵(60mg MAP)同时注射到三组中。第一组T1在拔出海绵前5天肌肉内注射氨基酸精氨酸(美国内华达州制造),直到拔出海绵后17天,而第二组T2在拔出海绵体前5天注射氨基酸(精氨酸),直到第17天。第三组T3对照组动物肌肉内注射5ml生理盐水。从放置海绵后第八天(取出海绵前五天)到取出海绵后17天,所有动物每天注射三次。其中注射总天数为22天。在海绵拔出后放置的海绵的第7天和第12天注射精氨酸之前,从颈静脉采集血样,分别在实验的第2天、第3天、第4天、第6天、第9天、第13天、第18天采集血样。本研究的目的是测量精氨酸治疗过程中血液生化参数的变化。这项研究的结果显示出显著的差异。T1和T2组的血液性质优于对照组,包括第2、5期的PCV,第2、3、4、5、6、9期的白细胞数量,第3、9期中的MID和第4、5,6、9期间的淋巴细胞,而第3期中的中性粒细胞。在生化特性方面,结果显示有显著差异,T1和T2处理在第6期的总蛋白浓度和第6期球蛋白浓度方面优于对照处理。我们得出结论,使用不同剂量的精氨酸可以改善母山羊的健康状况。
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引用次数: 0
The effect of hormonal treatment with kisspeptin, GnRH and hCG on semen characteristics in buck Cyprus goats during non-breeding season as compared with breeding season kisspeptin、GnRH和hCG激素处理对非繁殖季节和繁殖季节塞浦路斯公山羊精液特性的影响
Pub Date : 2019-06-20 DOI: 10.37940/AJVS.2019.12.1.9
M. AL-Ameri, T. Abdulkareem, A. Taha
The current study aimed to determine the effect of hormonal treatment with kisspeptin, GnRH and hCG on plasma testosterone concentration and semen characteristics in buck Cyprus goats during non-breeding season as compared with breeding season. This study was executed at the Ruminant Researches Station pertaining to the Directorate of Agricultural Researches, Ministry of Agriculture, Abu-Ghraib, Baghdad (latitude 33˚20' N) for the period from November 15th, 2012 to December 〖31〗^st 2013. A total of 20 buck Cyprus goats 2 years old and averages 55 kg body weight. During non-breeding season bucks were randomly divided into five equal groups (4 bucks / group). The first group (A1) was regarded as a control group, i.m injected with normal saline, whereas, the second (A2) and third (A3) groups were i.v injected with 4 and 8 µg / kg body weight of Kisspeptin-10 respectively. The fourth (A4) and fifth (A5) groups were i.m injected with hCG (250 IU / buck) and GnRH (20 µg / buck) respectively. Plasma testosterone concentrations were significantly (P≤ 0.01 and P≤ 0.05) increased at 20, 30, 40 and 50 min post-treatment in A5, A2 and A3 groups as compared with control A1. Mass motility and individual motility were significantly (P≤ 0.01) in A5, A4, A2 and A3 as compared with control during non-breeding season. In conclusion, our results show that Kisspeptin-10, GnRH and hCG can stimulate the quiescent hypothalamic–pituitary–gonadal axis of bucks during non-breeding season by increasing plasma testosterone concentration post treatment that leads improving some semen characteristics during non-breeding season.
本研究旨在确定kisspeptin、GnRH和hCG激素治疗对非繁殖期塞浦路斯公山羊血浆睾酮浓度和精液特征的影响,并与繁殖期进行比较。本研究于2012年11月15日至2013年12月31日在巴格达阿布格莱布市(纬度33˚20' N)农业部农业研究局反刍动物研究站进行。共20只2岁的塞浦路斯公山羊,平均体重55公斤。在非繁殖期,雄鹿被随机分为5组(每组4雄鹿)。第一组(A1)作为对照组,静脉注射生理盐水,第二组(A2)和第三组(A3)分别静脉注射Kisspeptin-10 4和8µg / kg体重。第四组(A4)和第五组(A5)分别注射hCG (250 IU / buck)和GnRH(20µg / buck)。治疗后20、30、40和50 min, A5、A2和A3组血浆睾酮浓度均显著高于对照A1 (P≤0.01和P≤0.05)。非繁殖期,A5、A4、A2和A3的质量活力和个体活力极显著(P≤0.01)高于对照。综上所述,Kisspeptin-10、GnRH和hCG可以通过提高雄鹿非繁殖期血浆睾酮浓度来刺激雄鹿非繁殖期静止的下丘脑-垂体-性腺轴,从而改善雄鹿非繁殖期的某些精液特征。
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引用次数: 4
Some heavy metals residues in beef and sheep Liver in Anbar province 安巴尔省牛肉和绵羊肝脏中的一些重金属残留
Pub Date : 2019-06-20 DOI: 10.37940/ajvs.2019.12.1.5
A. Saleh, Husam Nafee, H. Al-Nori
The aim of the study To see the effect of location, season and display time on the accumulation of some heavy metals (Lead, copper, zinc, cadmium and cobalt) in sheep and cattle liver. Liver samples were obtained from three districts in Anbar province (Ramadi, Hit and Baghdadi). This study began from January 2017 to November 2017 for all seasons (winter, spring, summer and autumn) Samples were taken from the liver immediately after slaughter for cattle and sheep at 8 am and taken at 4 pm for all areas of study. The results of the study were summarized in the quadratic overlap of the animal type, season, location and time as follows:The highest concentration of lead in liver (38.86 μg / g liver) was recorded in sheep for spring and evening in Ramadi. The lowest concentration was in sheep in Baghdadi for morning and winter (20.06 micro g / g liver). The highest concentration of copper in the liver (34.65 microgram / g liver) was recorded in cows in Ramadi for the winter season and evening time. The lowest concentration was in the sheep liver during the summer season for morning time in the city of al-Baghdadi (20.43 microgram / g liver). The highest concentration was in the liver (603.99 microgram / g liver) in the sheep for the autumn season and the evening time in the city of Ramadi. The lowest concentration was in the sheep in the city of Baghdadi for morning and summer (560.32 microgram / g liver). The highest concentration of cadmium in the liver (30.88 micro g / g liver) was in sheep in Ramadi for the autumn and evening season, and the lowest concentration was in beef in the summer season for morning time in al-Baghdadi city (9.66 μg / g liver). Cobalt was the highest concentration (1.34, 1.34 and 1.35 micro g / g liver) in cow and sheep liver, autumn, summer and evening time for Ramadi. While the lowest concentration of sheep and cattle liver for the city of Baghdadi for the winter season and morning time (0.64 and 0.63 microgram / g liver).
本研究的目的是观察地点、季节和展示时间对绵羊和牛肝脏中某些重金属(铅、铜、锌、镉和钴)积累的影响。肝脏样本取自安巴尔省的三个地区(拉马迪、希特和巴格达迪)。这项研究始于2017年1月至2017年11月,适用于所有季节(冬季、春季、夏季和秋季)。在上午8点屠宰牛和羊后立即从肝脏中采集样本,并在下午4点采集所有研究区域的样本。研究结果总结为动物类型、季节、地点和时间的二次重叠,如下所示:拉马迪春季和晚间绵羊的肝脏铅浓度最高(38.86μg/g肝脏)。巴格达迪的绵羊在早晨和冬天的浓度最低(20.06微克/克肝脏)。拉马迪的奶牛在冬季和夜间的肝脏中铜浓度最高(34.65微克/克肝脏)。巴格达迪市夏季早晨绵羊肝脏中的浓度最低(20.43微克/克肝脏)。拉马迪市秋季和夜间绵羊的肝脏浓度最高(603.99微克/克肝脏)。巴格达迪市的绵羊在早晨和夏天的浓度最低(560.32微克/克肝脏)。拉马迪市秋季和傍晚的绵羊肝脏镉浓度最高(30.88微克/克肝脏),巴格达迪市夏季早晨的牛肉肝脏镉浓度最低(9.66微克/克肝)。拉马迪的牛和绵羊肝脏、秋季、夏季和晚间的钴浓度最高(1.34、1.34和1.35微克/克肝脏)。而巴格达迪市冬季和早晨的绵羊和牛肝脏浓度最低(0.64和0.63微克/克肝脏)。
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引用次数: 2
Variation of egg proteins between bird varieties by using SDS-PAGE 利用SDS-PAGE分析鸟类品种间蛋蛋白的差异
Pub Date : 2019-06-20 DOI: 10.37940/AJVS.2019.12.1.8
Q. A. Amin, Hemn Zhahir, A. Shaker
Proteins are essential constituents of all organisms; both egg white proteins and egg yolk are source of protein. The aim of this study was conducted to perform preliminary studies to analyses and compare egg white proteins and yolk proteins from different avian species (guineafowl, dwarf hens, local hen, Shami, turkey, duck, geese, partridge and quail) via or with SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis ). 18 Fresh eggs of different poultry species (guineafowl, dwarf hens, local hen, Shami, turkey, duck, geese, partridge and quail) were collected from various farms in the Sulaimani province. Data on egg proteins were analyzed using Statistical Xlstate used for dendrogram construction and PCA. The main egg white proteins were Ovomicin, Ovotransferrin, Ovalbumin, Flavoprotein, α- chymotrypsinogen, and Trypsin inhibitor. The main lipoproteins were Apovitellenin VI, Apovitellenin Vb, Apovitellenin V, Apovitellenin IIIa, Apovitellenin III, Apovitellin 7, B-Livetin, Apovitellenin IIa, Apovitellenin II, and Apovitellenin I. All these lipoproteins were observed in the nine birds species. The egg white proteins and yolk lipoproteins for nine species were examined. It can be concluded the large differences were found in a mount of egg white proteins and yolk lipoproteins of the nine species of birds.
蛋白质是所有生物体的基本成分;蛋清和蛋黄都是蛋白质的来源。本研究的目的是通过SDS-PAGE(十二烷基硫酸钠-聚丙烯酰胺凝胶电泳)对不同鸟类(珍珠鸡、矮母鸡、地方母鸡、沙米鸡、火鸡、鸭、鹅、鹧鸪和鹌鹑)的蛋清蛋白和蛋黄蛋白进行初步分析和比较。从苏莱曼尼省的各个农场收集了18个不同家禽品种的新鲜鸡蛋(珍珠鸡、矮母鸡、当地母鸡、沙米鸡、火鸡、鸭、鹅、鹧鸪和鹌鹑)。使用Statistical Xlstate进行树突图构建和主成分分析。蛋清蛋白主要有卵黄蛋白、卵黄转铁蛋白、卵黄蛋白、α-凝乳胰蛋白酶原和胰蛋白酶抑制剂。主要脂蛋白有卵黄素VI、卵黄素Vb、卵黄素V、卵黄素IIIa、卵黄素III、卵黄素7、B-Livetin、卵黄素IIa、卵黄素II和卵黄素i。这些脂蛋白在9种鸟类中均有发现。测定了9种昆虫的蛋清蛋白和蛋黄脂蛋白。由此可见,9种鸟类的蛋清蛋白和蛋黄脂蛋白含量存在较大差异。
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