Pub Date : 2019-06-20DOI: 10.37940/AJVS.2019.12.1.3
I. Alawiy, T. Mohammed, A. Majeed
The study was conducted in one of the fields of the private sector within Basaaer the village of the district of Hit / Anbar at 180 km west of Baghdad, during the period from 17/4/2018 to 30/5/2018. 28 ewes with an age of 2-4 years with a weight of 40-50 kg with at least one birth. All ewes were tested using ultrasonography to ensure that they were free of pregnancy before the start of the experiment. Synchronization of estrus with intravaginal sponges was achieved in ewes, and inseminated with rams at the end of the hormonal program. The rams remained with the females for five days. The ewes were randomly divided into four equal groups (7 ewes in each group). The first group were injected with 160 micro mol / kg body weight and from the fifth day of the inseminated until day 20 of pregnancy The second group was treated by injecting selenium plus vitamin E with (2 ml/head α-Tocopheryl Acetate 68mg/ml Selenium 1.5mg/ml) intramuscular. The first dose was given on day 5 and the second one on day 19 of insemination the early at pregnancy. The third group were injected the arginine with the selenium plus vitamin E emulsion, The fourth group was injected with a physiological saline solution and considered as control group. Blood samples were collected from jugular vein prior to treatment on day 4 of the insemination as well as on days 10, 15, and 18, to measure the changes in the chemical properties at the early of pregnancy. The results of the study showed a significant difference (P≤0.05) between the treatments and control groups. The results showed a decrease in the concentration of urea and ALT enzyme as compared with the first group and control. The present study did not show a significant differences at early of pregnancy in the concentration of cholesterol, total protein, albumin, globulin, glucose, and AST enzyme at the early of pregnancy for ewes. The results also showed a significant difference (P≤0.05) between the different withdrawal periods within the same treatment in the control and other groups the concentration of ALT enzyme, and total protein. It can be concluded from this study that the use of arginine and selenium with vitamin E improves maternal health by reducing the level of urea and ALT enzyme and maintaining pregnancy after insemination.
{"title":"Effect of arginine and selenium with vitamin E in some biochemical properties of Inseminated ewes","authors":"I. Alawiy, T. Mohammed, A. Majeed","doi":"10.37940/AJVS.2019.12.1.3","DOIUrl":"https://doi.org/10.37940/AJVS.2019.12.1.3","url":null,"abstract":"The study was conducted in one of the fields of the private sector within Basaaer the village of the district of Hit / Anbar at 180 km west of Baghdad, during the period from 17/4/2018 to 30/5/2018. 28 ewes with an age of 2-4 years with a weight of 40-50 kg with at least one birth. All ewes were tested using ultrasonography to ensure that they were free of pregnancy before the start of the experiment. Synchronization of estrus with intravaginal sponges was achieved in ewes, and inseminated with rams at the end of the hormonal program. The rams remained with the females for five days. The ewes were randomly divided into four equal groups (7 ewes in each group). The first group were injected with 160 micro mol / kg body weight and from the fifth day of the inseminated until day 20 of pregnancy The second group was treated by injecting selenium plus vitamin E with (2 ml/head α-Tocopheryl Acetate 68mg/ml Selenium 1.5mg/ml) intramuscular. The first dose was given on day 5 and the second one on day 19 of insemination the early at pregnancy. The third group were injected the arginine with the selenium plus vitamin E emulsion, The fourth group was injected with a physiological saline solution and considered as control group. Blood samples were collected from jugular vein prior to treatment on day 4 of the insemination as well as on days 10, 15, and 18, to measure the changes in the chemical properties at the early of pregnancy. The results of the study showed a significant difference (P≤0.05) between the treatments and control groups. The results showed a decrease in the concentration of urea and ALT enzyme as compared with the first group and control. The present study did not show a significant differences at early of pregnancy in the concentration of cholesterol, total protein, albumin, globulin, glucose, and AST enzyme at the early of pregnancy for ewes. The results also showed a significant difference (P≤0.05) between the different withdrawal periods within the same treatment in the control and other groups the concentration of ALT enzyme, and total protein.\u0000 It can be concluded from this study that the use of arginine and selenium with vitamin E improves maternal health by reducing the level of urea and ALT enzyme and maintaining pregnancy after insemination.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46574245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-20DOI: 10.37940/ajvs.2019.12.1.6
M. Alkhilani
A two months old male Buffalo calf with a large abdominal swelling was brought to Baghdad Veterinary Hospital. From history, one month ago small swelling appeared at the lateral abdominal region and it was enlarged gradually with no effect on the appetite. On palpation it revealed irreducible swelling about volley ball in size. The hernial ring was at the upper flank region about four fingers in size and the small intestine was fall down to the lower abdominal region as a bocket between the abdominal wall and the skin. The case was successfully treated surgically by open herniorrhaphy.
{"title":"Abdominal Hernia in A Mesopotamia Buffalo calf","authors":"M. Alkhilani","doi":"10.37940/ajvs.2019.12.1.6","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.1.6","url":null,"abstract":"A two months old male Buffalo calf with a large abdominal swelling was brought to Baghdad Veterinary Hospital. From history, one month ago small swelling appeared at the lateral abdominal region and it was enlarged gradually with no effect on the appetite. On palpation it revealed irreducible swelling about volley ball in size. The hernial ring was at the upper flank region about four fingers in size and the small intestine was fall down to the lower abdominal region as a bocket between the abdominal wall and the skin. The case was successfully treated surgically by open herniorrhaphy.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48262693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-19DOI: 10.37940/AJVS.2019.12.1.10
Mohannad. K. Aremmt, T. Mohammed, S. Alrawi
This study was conducted to investigate the effect of (Saccharomyces cerevisiae) supported with zinc and selenium on the lipid profile. of Twenty-one Iraqi males sheep were used aged (1-1.5) years and the weight mean approximately (38±4) kg. experiment animals used divided randomly into three treated groups each group have 7 males. All males were fed on the same ration. The first group was considered as a control group without treatment (T1), the second group (T2) were given yeast enriched with zinc (Saccharomyces cerevisiae -zinc) with dosage (0.2 g/kg/bw), the third group (3) was given yeast enriched with selenium (Saccharomyces cerevisiae-selenium) with dosage (0.03 g/kg/bw). The results showed significant (P≤0.05) differences control group compare with the other two-treated groups (T3 and T2) in triglyceride, LDL , VLDL and HDL . The results indicate to significant differences (P≤0.05) of (T2 and T3) compare with the control group by HDL . From the results concluded that there synergistic effect of Saccharomyces cerevisiae with zinc and selenium as organic form (Saccharomyces cerevisiae-zinc/selenium) could be attributed to improved animal health.
{"title":"Effect of Yeast (Saccharomyces cerevisiae) Supported by Selenium and Zinc on Lipid Profile of Local Sheep Males","authors":"Mohannad. K. Aremmt, T. Mohammed, S. Alrawi","doi":"10.37940/AJVS.2019.12.1.10","DOIUrl":"https://doi.org/10.37940/AJVS.2019.12.1.10","url":null,"abstract":"This study was conducted to investigate the effect of (Saccharomyces cerevisiae) supported with zinc and selenium on the lipid profile. of Twenty-one Iraqi males sheep were used aged (1-1.5) years and the weight mean approximately (38±4) kg. experiment animals used divided randomly into three treated groups each group have 7 males. All males were fed on the same ration. The first group was considered as a control group without treatment (T1), the second group (T2) were given yeast enriched with zinc (Saccharomyces cerevisiae -zinc) with dosage (0.2 g/kg/bw), the third group (3) was given yeast enriched with selenium (Saccharomyces cerevisiae-selenium) with dosage (0.03 g/kg/bw). The results showed significant (P≤0.05) differences control group compare with the other two-treated groups (T3 and T2) in triglyceride, LDL , VLDL and HDL . The results indicate to significant differences (P≤0.05) of (T2 and T3) compare with the control group by HDL . From the results concluded that there synergistic effect of Saccharomyces cerevisiae with zinc and selenium as organic form (Saccharomyces cerevisiae-zinc/selenium) could be attributed to improved animal health.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49559113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.37940/AJVS.2019.12.1.14
A. Mahmud, H. H. Saleh, H. Zahir
This study was carried out to develop a method for enhancing tenderness and overall qualities of Karadi tough aged bull meat (more than 5 years old) by using a proteolytic extract from cucumis fruit (Cucumis trigonus Rox-b) compared to papain enzyme. After slaughter bull and dressing carcass, the three main muscle, longissimus dorsi (LD), semimembranosus (SM) and supraspinatus (SS) were evaluated by injecting brine supplied with extract solutions in different concentrations. (0.1, 0.2 and 0.3 % of cold cucumis extract solution and 0.02 % of Papain extract solution and distilled water as a group) at an average of injection of 10 % muscle weight. Overall, there were significant (p˂0.05) reduction of muscle pH, water holding capacity and cooking loss and significant (p˂0.05) increase in collagen solubility, nitrogen solubility in LD, SM and SS muscle samples treated with cucumis extract compared with control and Papain enzyme. The increased concentration of cucumis extract resulted in a significant increase (p˂0.05) in total and myofibrillar protein solubility with a slight increase of sarcoplasmic solubility in muscle samples that treated with cucumis extract compared to control and Papain enzyme. The electrophoresis pattern of muscle treated samples also revealed extensive proteolysis occurring in each muscle type. The results of the sensory evaluation indicated that the tenderness, juiciness and overall acceptability of all treated muscle samples significantly (p<0.05) improved compared with control. In our experiment, generally, cucumis extract tended to be more effective than papain extract for most of the studied traits. Therefor it can be summarized that cucumis extract is one of the best alternative source of proteolytic enzymes for the effective tenderizing of meat.
{"title":"Tenderization Efficiency of Cucumis Extract (Cucumis Trigonus Rox-b) Compared with Papain enzyme on the Aged Bull Meat by Injection Method","authors":"A. Mahmud, H. H. Saleh, H. Zahir","doi":"10.37940/AJVS.2019.12.1.14","DOIUrl":"https://doi.org/10.37940/AJVS.2019.12.1.14","url":null,"abstract":"This study was carried out to develop a method for enhancing tenderness and overall qualities of Karadi tough aged bull meat (more than 5 years old) by using a proteolytic extract from cucumis fruit (Cucumis trigonus Rox-b) compared to papain enzyme. After slaughter bull and dressing carcass, the three main muscle, longissimus dorsi (LD), semimembranosus (SM) and supraspinatus (SS) were evaluated by injecting brine supplied with extract solutions in different concentrations. (0.1, 0.2 and 0.3 % of cold cucumis extract solution and 0.02 % of Papain extract solution and distilled water as a group) at an average of injection of 10 % muscle weight. Overall, there were significant (p˂0.05) reduction of muscle pH, water holding capacity and cooking loss and significant (p˂0.05) increase in collagen solubility, nitrogen solubility in LD, SM and SS muscle samples treated with cucumis extract compared with control and Papain enzyme. The increased concentration of cucumis extract resulted in a significant increase (p˂0.05) in total and myofibrillar protein solubility with a slight increase of sarcoplasmic solubility in muscle samples that treated with cucumis extract compared to control and Papain enzyme. The electrophoresis pattern of muscle treated samples also revealed extensive proteolysis occurring in each muscle type. The results of the sensory evaluation indicated that the tenderness, juiciness and overall acceptability of all treated muscle samples significantly (p<0.05) improved compared with control. In our experiment, generally, cucumis extract tended to be more effective than papain extract for most of the studied traits. Therefor it can be summarized that cucumis extract is one of the best alternative source of proteolytic enzymes for the effective tenderizing of meat.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44560163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.37940/AJVS.2019.12.1.12
Nawfal Mutlak
The aim of this study was to examine the effect of different concentrations of egg yolk EY (0%, 10%, and 20%) in the semen extender during the cryopreservation process of goat semen out of the breeding season. A total of 12 ejaculates were collected from six Anglo Nubain dairy bucks as two ejaculates for each buck aged between (1-5) years over a two week period by using Electro-ejaculation (EEJ) during the non-breeding season. Post collection, the semen samples were evaluated for motility and mass activity. Subsequently, the semen samples were initially diluted in Tris solution (without Egg yolk or Glycerol) in order to preserve the motility of sperm cells. The semen samples from each buck were evaluated for pre-freezing motility and morphology then divided into three sub-samples and diluted in Tris extender with T1 (control) 0% EY, T2 10% EY, and T3 20% EY. The semen samples were frozen in liquid nitrogen (-196 C). After thawing, the semen samples were evaluated for sperm motility and morphology. The morphology of sperm did not differ among treatments nor between pre-freezing and post-thawing evaluations. However, the motility of semen diluted with 10% EY was (P<0.05) numerically but not statistically higher than semen diluted with 0% and 20% EY. According to the obtained results of this study, it is recommended that a 10% EY level or less be included in the Tris extender during cryopreservation of goat semen for superior motility and morphology results.
{"title":"The impact of adding different levels of egg yolk on the motility and morphology pre and post thaw cryopreservation of goat semen","authors":"Nawfal Mutlak","doi":"10.37940/AJVS.2019.12.1.12","DOIUrl":"https://doi.org/10.37940/AJVS.2019.12.1.12","url":null,"abstract":"The aim of this study was to examine the effect of different concentrations of egg yolk EY (0%, 10%, and 20%) in the semen extender during the cryopreservation process of goat semen out of the breeding season. A total of 12 ejaculates were collected from six Anglo Nubain dairy bucks as two ejaculates for each buck aged between (1-5) years over a two week period by using Electro-ejaculation (EEJ) during the non-breeding season. Post collection, the semen samples were evaluated for motility and mass activity. Subsequently, the semen samples were initially diluted in Tris solution (without Egg yolk or Glycerol) in order to preserve the motility of sperm cells. The semen samples from each buck were evaluated for pre-freezing motility and morphology then divided into three sub-samples and diluted in Tris extender with T1 (control) 0% EY, T2 10% EY, and T3 20% EY. The semen samples were frozen in liquid nitrogen (-196 C). After thawing, the semen samples were evaluated for sperm motility and morphology. The morphology of sperm did not differ among treatments nor between pre-freezing and post-thawing evaluations. However, the motility of semen diluted with 10% EY was (P<0.05) numerically but not statistically higher than semen diluted with 0% and 20% EY. According to the obtained results of this study, it is recommended that a 10% EY level or less be included in the Tris extender during cryopreservation of goat semen for superior motility and morphology results.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43433921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.37940/AJVS.2019.12.1.11
Alaa Nazar
Ricinus communis L. of Euphorbiaceae family is a widespread plant in tropical regions. It is used in traditional medicines as an anti- fertility agent in India and different parts of the world. The ether soluble portion of the methanol extract of R. communis var minor possesses anti-implantation, anti - conceptive and estrogenic activity in rats and mice when administered subcutaneously.The study was conducted on 10 local breed male rabbits, 1-2 years old, of 1-2 kg body weight. The animals were divided into two groups, control non – treated group and treated group in which animals were treated with single daily dose of 50 mg /kg b. wt. P.O. of decorticated and defatted castor seeds (DDCS) for 14 days. 28th day post treatment, animals were anesthetized by diethyl ether, sacrificed, abdominal cavity was open. The sexual organ (testes, epididymis, prostate and seminal vesical) weighed. In addition to take a biopsy from each one for histopathological changes. The study also included clinical and hematological parameters, in addition to sperm counts and the changes in sperm morphology.Body weight, body temperature increased significantly in treated males. While in non- treated group there were no significant changes. Respiratory rates and heart rate were none significantly changed in treated and non- treated males.Bleeding time none significantly increased in treated males, but increased significantly in none treated males. Clotting times decreased none significantly in treated and non- treated males. The blood parameters including, total erythrocytes count, hemoglobin concentration, PCV% , MCV, MCH, MCHC, total leucocyte and differential leucocyte counts were either increased or decreased none significantly in both groups. The results revealed that the effects of exposure to extract of ricin for 14 days on reproductive efficiency of rabbits, exhibited Significant decrease in weights of testes, epididymis, tails, heads of epididymis, seminal vesicles and prostate in treated males in comparison with those of non- treated males. While the body of epididymis did not show a significant changes.Significant decrease in live sperm numbers, number of sperms in epididymal head, in addition to deformities in high numbers of sperm, including enlarged or small sperms. breaks head, and its detachment, presence of two heads in one sperm, bifurcation of tail and its breaking, sperm coiling in samples from treated males in comparison with those from non-treated males.Histological changes were hyperplasia of lining epithelial cells and vacuolar degenerative changes, loss of spermatogenesis, and spermatocytes necrosis in those from treated males.
大戟科蓖麻属(Ricinus communis L.)是热带地区广泛分布的植物。在印度和世界其他地区，它在传统医药中被用作抗生育剂。小檗甲醇提取物醚溶性部分经皮下注射后具有抗着床、抗受孕和雌激素活性。试验选用10只1 ~ 2岁、1 ~ 2 kg体重的地方品种公兔。将实验动物分为两组，对照组、未处理组和处理组，处理组每天给药50 mg /kg b. wt. P.O.去皮去脂蓖麻籽(DDCS)，连续14 d。治疗后第28天，用乙醚麻醉，处死，切开腹腔。性器官(睾丸、附睾、前列腺和精囊)称重。除此之外，还要对每个人进行活检，观察组织病理学变化。该研究还包括临床和血液学参数，以及精子数量和精子形态的变化。处理过的雄鼠体重、体温显著升高。而非治疗组无明显变化。治疗组和非治疗组的呼吸频率和心率无显著变化。治疗组男性出血时间无显著增加，但未治疗组男性出血时间显著增加。治疗组和未治疗组的男性凝血时间均无显著减少。两组患者外周血总红细胞计数、血红蛋白浓度、PCV%、MCV、MCH、MCHC、总白细胞计数和差异白细胞计数均无显著升高或降低。结果表明，蓖麻毒素提取物对家兔生殖效率的影响表明，与未处理家兔相比，处理家兔的睾丸、附睾、尾部、附睾头、精囊和前列腺重量显著降低。而附睾体未见明显变化。活精子数量显著减少，附睾头部精子数量显著减少，除此之外，大量精子畸形，包括精子增大或变小。在接受过治疗的雄性和未接受过治疗的雄性样本中，头部断裂及其脱离，一个精子中存在两个头部，尾部分叉及其断裂，精子盘绕。组织学改变为上皮细胞内膜增生和空泡变性改变，精子发生丧失和精母细胞坏死。
{"title":"The effect of Ricinus communis seeds extract on reproductive \u0000activity and blood values of male rabbits","authors":"Alaa Nazar","doi":"10.37940/AJVS.2019.12.1.11","DOIUrl":"https://doi.org/10.37940/AJVS.2019.12.1.11","url":null,"abstract":"Ricinus communis L. of Euphorbiaceae family is a widespread plant in tropical regions. It is used in traditional medicines as an anti- fertility agent in India and different parts of the world. The ether soluble portion of the methanol extract of R. communis var minor possesses anti-implantation, anti - conceptive and estrogenic activity in rats and mice when administered subcutaneously.The study was conducted on 10 local breed male rabbits, 1-2 years old, of 1-2 kg body weight. The animals were divided into two groups, control non – treated group and treated group in which animals were treated with single daily dose of 50 mg /kg b. wt. P.O. of decorticated and defatted castor seeds (DDCS) for 14 days. 28th day post treatment, animals were anesthetized by diethyl ether, sacrificed, abdominal cavity was open. The sexual organ (testes, epididymis, prostate and seminal vesical) weighed. In addition to take a biopsy from each one for histopathological changes. The study also included clinical and hematological parameters, in addition to sperm counts and the changes in sperm morphology.Body weight, body temperature increased significantly in treated males. While in non- treated group there were no significant changes. Respiratory rates and heart rate were none significantly changed in treated and non- treated males.Bleeding time none significantly increased in treated males, but increased significantly in none treated males. Clotting times decreased none significantly in treated and non- treated males. The blood parameters including, total erythrocytes count, hemoglobin concentration, PCV% , MCV, MCH, MCHC, total leucocyte and differential leucocyte counts were either increased or decreased none significantly in both groups. The results revealed that the effects of exposure to extract of ricin for 14 days on reproductive efficiency of rabbits, exhibited Significant decrease in weights of testes, epididymis, tails, heads of epididymis, seminal vesicles and prostate in treated males in comparison with those of non- treated males. While the body of epididymis did not show a significant changes.Significant decrease in live sperm numbers, number of sperms in epididymal head, in addition to deformities in high numbers of sperm, including enlarged or small sperms. breaks head, and its detachment, presence of two heads in one sperm, bifurcation of tail and its breaking, sperm coiling in samples from treated males in comparison with those from non-treated males.Histological changes were hyperplasia of lining epithelial cells and vacuolar degenerative changes, loss of spermatogenesis, and spermatocytes necrosis in those from treated males.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46145230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.2
O. Hammoodi, S. I. Salih
The present study was planned to evaluate the efficacy of tendon derived hydrogel and hyaluronic acid in repair of experimentally induced superficial digital flexor tendon defect in rams, ultrasonographically with detection of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in blood. Thirty six adult rams were divided randomly into three equal groups: First group (control group) rams were subjected to partial removal of the superficial digital flexor tendon (SDFT), second group (tendon derived hydrogel group): rams were subjected to the same procedure as mentioned in first group except that the tendon defect was filled with tendon derived hydrogel directly, while third group (hyaluronic acid group): same procedure as in first group but the tendon defect was filled with hyaluronic acid directly. Clinically there was no pain, no infection, no signs of rejection and all wounds had healed completely in all rams, normal body temperature, except for the swelling which appeared in the first group in one ram at 30 days post operatively and two rams in third group at 60 days post operatively, all animals were able to stand and showed normal gait. Ultrasonographic results at 120th day post operation in first group showed, hypoechoic to hyperechoic regions at the site of the defect. At the same period for the second group the sonogram showed, well-aligned echoic tendon structure, while in the third group at the same period there were hypoechoic areas at the site of the defect. The results of bFGF and VEGF detection showed significant differences between the periods in first group at P≤0.05, while in second and third groups there were significant differences at P≤0.01for bFGF. The results of detection VEGF showed, significant differences in the mean values among the periods at P≤0.01in first group, also second group there was significant differences at P≤0.01. And in third group there was a significant difference at P≤0.05. Also there were significant differences among all groups P≤0.01in at 60th day postoperatively. In conclusion the tendon derived hydrogel matrix and the hyaluronic acid have a regenerative effect in repair of tendons injuries, but the superiority is for tendon derived hydrogel matrix.
{"title":"Ultra-Sonographic Evaluation and Growth Factors Measurement of Using Tendon Derived Hydrogel and Hyaluronic Acid in Tendon Repair in Rams","authors":"O. Hammoodi, S. I. Salih","doi":"10.37940/ajvs.2019.12.2.2","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.2","url":null,"abstract":"The present study was planned to evaluate the efficacy of tendon derived hydrogel and hyaluronic acid in repair of experimentally induced superficial digital flexor tendon defect in rams, ultrasonographically with detection of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in blood. Thirty six adult rams were divided randomly into three equal groups: First group (control group) rams were subjected to partial removal of the superficial digital flexor tendon (SDFT), second group (tendon derived hydrogel group): rams were subjected to the same procedure as mentioned in first group except that the tendon defect was filled with tendon derived hydrogel directly, while third group (hyaluronic acid group): same procedure as in first group but the tendon defect was filled with hyaluronic acid directly. Clinically there was no pain, no infection, no signs of rejection and all wounds had healed completely in all rams, normal body temperature, except for the swelling which appeared in the first group in one ram at 30 days post operatively and two rams in third group at 60 days post operatively, all animals were able to stand and showed normal gait. Ultrasonographic results at 120th day post operation in first group showed, hypoechoic to hyperechoic regions at the site of the defect. At the same period for the second group the sonogram showed, well-aligned echoic tendon structure, while in the third group at the same period there were hypoechoic areas at the site of the defect. The results of bFGF and VEGF detection showed significant differences between the periods in first group at P≤0.05, while in second and third groups there were significant differences at P≤0.01for bFGF. The results of detection VEGF showed, significant differences in the mean values among the periods at P≤0.01in first group, also second group there was significant differences at P≤0.01. And in third group there was a significant difference at P≤0.05. Also there were significant differences among all groups P≤0.01in at 60th day postoperatively. In conclusion the tendon derived hydrogel matrix and the hyaluronic acid have a regenerative effect in repair of tendons injuries, but the superiority is for tendon derived hydrogel matrix.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70217885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.8
Hadeel Asim Mohammed, E. D. Hadi, D. Moosa, Ahmed Nawzad Flyyih
The objective of the present study was to determine serum copper concentrations in sheep and goats infected with different gastrointestinal parasites. A total of 60 sheep and 30 goats were obtained from different areas in Mosul city and examined in the Veterinary Teaching Hospital, University of Mosul. Fecal and blood samples were collected from each animal. Animals infected with gastrointestinal parasites were diagnosed by detecting parasite eggs in fecal samples. Copper concentrations and hematological values were compared between infected and non-infected animals. Different gastrointestinal parasites were diagnosed include; Chabertia ovina, Cooperia spp., Haemonchus contortus, Marshallagia marshalli, Nematodirus spp., Oesophagostomum spp., Ostertagia ostertagia, Strongylus spp. and Trichostrongylus spp. in both single and mixed infection types. Infected sheep and goats suffered from different clinical signs such as emaciation, poor wool or hair, trembling, pale mucous membrane, loss of appetite, and diarrhea. Infected animals showed significantly (p≤0.05) lower serum copper concentrations compared to non-infected animals. Moreover, hematological parameters (RBC, Hb, PCV, MCH, MCV, and MCHC) were lower compared to non-infected animals. In conclusion, gastrointestinal parasitic infection in sheep and goats can decrease serum copper concentrations and blood parameters in infected animals, thereby exacerbating clinical signs.
{"title":"Estimation of Serum Copper Concentrations in Sheep and Goats Infected with Gastrointestinal Parasites in Mosul City, Iraq","authors":"Hadeel Asim Mohammed, E. D. Hadi, D. Moosa, Ahmed Nawzad Flyyih","doi":"10.37940/ajvs.2019.12.2.8","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.8","url":null,"abstract":"The objective of the present study was to determine serum copper concentrations in sheep and goats infected with different gastrointestinal parasites. A total of 60 sheep and 30 goats were obtained from different areas in Mosul city and examined in the Veterinary Teaching Hospital, University of Mosul. Fecal and blood samples were collected from each animal. Animals infected with gastrointestinal parasites were diagnosed by detecting parasite eggs in fecal samples. Copper concentrations and hematological values were compared between infected and non-infected animals. Different gastrointestinal parasites were diagnosed include; Chabertia ovina, Cooperia spp., Haemonchus contortus, Marshallagia marshalli, Nematodirus spp., Oesophagostomum spp., Ostertagia ostertagia, Strongylus spp. and Trichostrongylus spp. in both single and mixed infection types. Infected sheep and goats suffered from different clinical signs such as emaciation, poor wool or hair, trembling, pale mucous membrane, loss of appetite, and diarrhea. Infected animals showed significantly (p≤0.05) lower serum copper concentrations compared to non-infected animals. Moreover, hematological parameters (RBC, Hb, PCV, MCH, MCV, and MCHC) were lower compared to non-infected animals. In conclusion, gastrointestinal parasitic infection in sheep and goats can decrease serum copper concentrations and blood parameters in infected animals, thereby exacerbating clinical signs.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70218230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.7
Sazan Qadr Amin, N. Abdulrahman, S. Aziz
The study is conducting to isolate and toxinotype the suspected cases of Clostridium perfringens infections of broiler farms in Sulaimania province. A total of 108 samples were collected from intestinal contents, mucosal scraping, and hemorrhagic lymphoid nodules from suspected cases of necrotic enteritis in broilers. The result of isolated and identified bacteria were revealed that 63 (58%) out of 108 samples were positive for C. perfringens. The results revealed that the isolates were only positive for alpha and beta2 toxin genes. Phylogenetic and DNA sequence analysis of cpa and cpb2 gene showed that cpa genes were highly identical to isolates from broiler in Iran, poultry stool and broiler in Brazil, and blue calves in Belgium. While cpb2 gene is closely related to the isolates of broiler in Iran, India and isolates of goat in Pakistan. The results indicated that the causative agent of necrotic enteritis in broiler farms in the region was mainly due to C. perfringens type A infection
{"title":"Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province","authors":"Sazan Qadr Amin, N. Abdulrahman, S. Aziz","doi":"10.37940/ajvs.2019.12.2.7","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.7","url":null,"abstract":"The study is conducting to isolate and toxinotype the suspected cases of Clostridium perfringens infections of broiler farms in Sulaimania province. A total of 108 samples were collected from intestinal contents, mucosal scraping, and hemorrhagic lymphoid nodules from suspected cases of necrotic enteritis in broilers. The result of isolated and identified bacteria were revealed that 63 (58%) out of 108 samples were positive for C. perfringens. The results revealed that the isolates were only positive for alpha and beta2 toxin genes. Phylogenetic and DNA sequence analysis of cpa and cpb2 gene showed that cpa genes were highly identical to isolates from broiler in Iran, poultry stool and broiler in Brazil, and blue calves in Belgium. While cpb2 gene is closely related to the isolates of broiler in Iran, India and isolates of goat in Pakistan. The results indicated that the causative agent of necrotic enteritis in broiler farms in the region was mainly due to C. perfringens type A infection","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70218649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.13
A. Omar, T. Mohammed, Salah M. A. Al-Kubaisi
This study was conducted to determine the effect of bone marrow stromal cells (BMSCs) on progesterone level during pregnancy in ewes. Flurogestone Acetate Sponges 40 mg, followed by 400 i.u. Equine chorionic gonadotropin (eCG) were used to synchronize estrus and ovulation. The animals were divided into three equal groups (5 animals for each group). The 1st and 2nd group injected intravenously after eCG injection with 1x108 and 2x108 respectively with BMSCs while the 3rd group was injected with normal saline which serve as a control group. Blood samples were collected during pregnancy, at day 10, 21, 85 from the jugular vein. At day 10, 21 the results showed that there was a significant difference (p≤0.05) in the level of progesterone between treated groups as compared with the control group. While there was no significant difference between different groups at day 85. It was concluded from this study that BMSCs have a beneficial effect in ewe’s reproductive system, by increasing the level of progesterone at early pregnancy.
{"title":"Progesterone Levels During Pregnancy in Ewes Treated with Bone Marrow Stromal Cells","authors":"A. Omar, T. Mohammed, Salah M. A. Al-Kubaisi","doi":"10.37940/ajvs.2019.12.2.13","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.13","url":null,"abstract":"This study was conducted to determine the effect of bone marrow stromal cells (BMSCs) on progesterone level during pregnancy in ewes. Flurogestone Acetate Sponges 40 mg, followed by 400 i.u. Equine chorionic gonadotropin (eCG) were used to synchronize estrus and ovulation. The animals were divided into three equal groups (5 animals for each group). The 1st and 2nd group injected intravenously after eCG injection with 1x108 and 2x108 respectively with BMSCs while the 3rd group was injected with normal saline which serve as a control group. Blood samples were collected during pregnancy, at day 10, 21, 85 from the jugular vein. At day 10, 21 the results showed that there was a significant difference (p≤0.05) in the level of progesterone between treated groups as compared with the control group. While there was no significant difference between different groups at day 85. It was concluded from this study that BMSCs have a beneficial effect in ewe’s reproductive system, by increasing the level of progesterone at early pregnancy.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70217678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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