Pub Date : 2024-05-01DOI: 10.30595/medisains.v22i1.21357
Faridah BD Faridah BD, Lita Angelina Saputri, Iin Prima Fitriah, Lisa Rahmawati, N. Oktavia, Fithriani Armin
Background: The COVID-19 outbreak has changed how people live worldwide, making hand hygiene a must, whether it involves hand sanitizer (HS). The majority of HS are made of alcohol. Several side effects of alcohol are dryness and allergies. Alternative natural ingredients and HS are needed. Red betel (Piper crocatum) and white galangal (Alpinia galanga) are familiar and easy to be found. These two plants have the potential to be the composition of HS because of their compounds, such as antioxidants, antibiotics, antifungals, and antivirals. However, there is a need for further research on their formulation and effectiveness in germ-killing.Purpose: To formulate and identify the antibacterial activity of HS containing red betel and white galangal extract.Method: This research was an in vitro laboratory experiment with several steps, including extraction, specific and nonspecific tests, formulation, and antibacterial activity tests.Results: Red betel and white galangal leaves had been extracted at a concentration of 10% and formulated into HS. The HS containing 10% red betel extract produced an inhibition zone of 0 mm against E. coli and 1.05 mm against S. aureus, and the HS containing 10% White galangal extract produced an inhibition zone of 1.47 mm against E. coli and 1.40 mm against S. aureus.Conclusion: The formulation of HS containing 10% of white galangal extract is more effective than the HS containing 10% of Red betel extract.
{"title":"Formulation and evaluation of antibacterial activity of hand sanitizers containing red betel (Piper crocatum) and white galangal (Alpinia galanga) extract","authors":"Faridah BD Faridah BD, Lita Angelina Saputri, Iin Prima Fitriah, Lisa Rahmawati, N. Oktavia, Fithriani Armin","doi":"10.30595/medisains.v22i1.21357","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.21357","url":null,"abstract":"Background: The COVID-19 outbreak has changed how people live worldwide, making hand hygiene a must, whether it involves hand sanitizer (HS). The majority of HS are made of alcohol. Several side effects of alcohol are dryness and allergies. Alternative natural ingredients and HS are needed. Red betel (Piper crocatum) and white galangal (Alpinia galanga) are familiar and easy to be found. These two plants have the potential to be the composition of HS because of their compounds, such as antioxidants, antibiotics, antifungals, and antivirals. However, there is a need for further research on their formulation and effectiveness in germ-killing.Purpose: To formulate and identify the antibacterial activity of HS containing red betel and white galangal extract.Method: This research was an in vitro laboratory experiment with several steps, including extraction, specific and nonspecific tests, formulation, and antibacterial activity tests.Results: Red betel and white galangal leaves had been extracted at a concentration of 10% and formulated into HS. The HS containing 10% red betel extract produced an inhibition zone of 0 mm against E. coli and 1.05 mm against S. aureus, and the HS containing 10% White galangal extract produced an inhibition zone of 1.47 mm against E. coli and 1.40 mm against S. aureus.Conclusion: The formulation of HS containing 10% of white galangal extract is more effective than the HS containing 10% of Red betel extract.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141025062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Treating diabetic foot ulcers (DFU) sometimes takes a long time. During treatment, the patient's feet are usually given a cushion (such as a sleeping pillow or bolster) to make the wound seen. This way, some patients reported that their feet tingled because they were not positioned rightly for an extended period. So far, no unique pillow is available for this function. This pillow would help in treating DFU and should be comfortable for patients. Thus, it is essential to research designing such a product.Purpose: To develop and test a special pillow for treating DFU.Methods: This research adopts Research and Development (R&D). This research is divided into two stages: designing and developing and product testing.Results: This pillow is made of multiplex and foam, covered with Polyester fabric. It is 75mm in length and 45mm in width. It is equipped with a foot care area and waste storage tank. The test results showed that the score for comfort for the patients was 92%, while the score from the nurse was 88%.Conclusion:The pillow product helps the nurse conduct the DFU treatment and is comfortable for the patients.
{"title":"An innovative support pillow for diabetic foot wound care","authors":"Jebul Suroso, Aqilatun Zulfa, Nanda Cikal Gumelar, Rama Nanda Soleha, Siti Nuraeni, Ika Putri Febriyanti, Agus Santosa","doi":"10.30595/medisains.v22i1.21428","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.21428","url":null,"abstract":"Background: Treating diabetic foot ulcers (DFU) sometimes takes a long time. During treatment, the patient's feet are usually given a cushion (such as a sleeping pillow or bolster) to make the wound seen. This way, some patients reported that their feet tingled because they were not positioned rightly for an extended period. So far, no unique pillow is available for this function. This pillow would help in treating DFU and should be comfortable for patients. Thus, it is essential to research designing such a product.Purpose: To develop and test a special pillow for treating DFU.Methods: This research adopts Research and Development (R&D). This research is divided into two stages: designing and developing and product testing.Results: This pillow is made of multiplex and foam, covered with Polyester fabric. It is 75mm in length and 45mm in width. It is equipped with a foot care area and waste storage tank. The test results showed that the score for comfort for the patients was 92%, while the score from the nurse was 88%.Conclusion:The pillow product helps the nurse conduct the DFU treatment and is comfortable for the patients. ","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141028764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.30595/medisains.v22i1.21484
Anis Wiji Astuti, F. Rosyid, S. Subrata
Background: Digital subtraction angiography can affect anxiety in patients so that it can worsen the patient's condition during catheterization, such as increased heart rate and breathing. One of the efforts to reduce anxiety that can improve hemodynamics is butterfly hug therapy. However, its effectiveness in reducing hemodynamic anxiety needs to be further investigated.Purpose: To evaluate butterfly hug therapy's effectiveness on anxiety and hemodynamics before digital subtraction angiography.Methods: This is a quasi-experiment study with a pretest-posttest control group design. Fifty-two respondents were selected using purposive sampling. A total of 26 respondents were given butterfly hug therapy, and 26 others were given standard therapy. The study implemented the Zung Self Rating Anxiety Scale Questionnaire and observation sheet. This research analysis uses the Independent T-test and Wilcoxon Test.Results: The anxiety level of the experimental group was lower than that of the control group (46.73 ± 2.08 vs. 53.84 ± 1.74; p<0.05). In hemodynamic stability (pulse, respiratory, and blood pressure) was stability than the control group (80.69 ± 2.44 vs. 89.11 ± 2.39; 20.65 ± 0.39 vs. 21.0 ± 0.53; 104.7 ± 1.46 vs. 109.5 ± 1.82; p <0.05).Conclusion: Butterfly hug therapy is effective in reducing anxiety and stabilizing hemodynamics among patients undergoing digital subtraction angiography.
背景：数字减影血管造影术会影响患者的焦虑情绪，从而导致导管插入术中患者的病情恶化，如心率和呼吸加快。蝴蝶拥抱疗法是减轻焦虑、改善血液动力学的方法之一。目的：评估蝴蝶拥抱疗法对数字减影血管造影术前焦虑和血液动力学的影响：这是一项采用前测-后测对照组设计的准实验研究。采用目的性抽样法选出了 52 名受访者。共有 26 名受访者接受了蝴蝶拥抱疗法，另有 26 名受访者接受了标准疗法。研究采用了 Zung 焦虑自评量表问卷和观察表。研究分析采用独立 T 检验和 Wilcoxon 检验：实验组的焦虑水平低于对照组（46.73 ± 2.08 vs. 53.84 ± 1.74；P<0.05）。在血液动力学稳定性（脉搏、呼吸和血压）方面，实验组比对照组稳定（80.69 ± 2.44 vs. 89.11 ± 2.39；20.65 ± 0.39 vs. 21.0 ± 0.53；104.7 ± 1.46 vs. 109.5 ± 1.82；P <0.05）：结论：蝶形拥抱疗法能有效减轻接受数字减影血管造影术的患者的焦虑情绪并稳定血流动力学。
{"title":"Butterfly hug therapy on reducing anxiety levels and stabilization hemodynamics in patient digital subtraction angiography","authors":"Anis Wiji Astuti, F. Rosyid, S. Subrata","doi":"10.30595/medisains.v22i1.21484","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.21484","url":null,"abstract":"Background: Digital subtraction angiography can affect anxiety in patients so that it can worsen the patient's condition during catheterization, such as increased heart rate and breathing. One of the efforts to reduce anxiety that can improve hemodynamics is butterfly hug therapy. However, its effectiveness in reducing hemodynamic anxiety needs to be further investigated.Purpose: To evaluate butterfly hug therapy's effectiveness on anxiety and hemodynamics before digital subtraction angiography.Methods: This is a quasi-experiment study with a pretest-posttest control group design. Fifty-two respondents were selected using purposive sampling. A total of 26 respondents were given butterfly hug therapy, and 26 others were given standard therapy. The study implemented the Zung Self Rating Anxiety Scale Questionnaire and observation sheet. This research analysis uses the Independent T-test and Wilcoxon Test.Results: The anxiety level of the experimental group was lower than that of the control group (46.73 ± 2.08 vs. 53.84 ± 1.74; p<0.05). In hemodynamic stability (pulse, respiratory, and blood pressure) was stability than the control group (80.69 ± 2.44 vs. 89.11 ± 2.39; 20.65 ± 0.39 vs. 21.0 ± 0.53; 104.7 ± 1.46 vs. 109.5 ± 1.82; p <0.05).Conclusion: Butterfly hug therapy is effective in reducing anxiety and stabilizing hemodynamics among patients undergoing digital subtraction angiography.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141041341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.30595/medisains.v22i1.19531
Elviga Arselatifa, Sri Sumarni, Kurnianingsih Kurnianingsih
Introduction: Most cases of perinatal asphyxia are caused by conditions unrelated to labor. When asphyxia occurs during childbirth, it is usually caused by an obstetric emergency that was not detected during pregnancy. It is essential to prevent asphyxia by identifying the incidence of asphyxia during pregnancy. Several studies have been conducted to identify asphyxia problems developing by predictive models. However, there has been no development of a system for predicting birth asphyxia during pregnancy and carried out in primary health facilities.Purpose: Develop a web-based system using the Naïve Bayes (NB) algorithm to predict asphyxia neonatal using a dataset of antepartum risk factors in primary health facilities.Methods: This study employed research and development, which consists of 4 stages, namely literature study, development stage, expert validity, and trial.Results: A system that health workers in primary health facilities can use to predict asphyxia neonatal and recommend referrals for determining the place of childbirth has been successfully created. The system performance test predicted asphyxia neonatal with all NB evaluation values reaching more than 98%, and the prediction accuracy in the respondent test included in the High Accuracy category (MAPE value 9.06%).Conclusion: The development of a web-based system using the NB algorithm has been proven to be able to predict asphyxia neonatal and can be implemented for health workers as an effort to anticipate delays in handling cases of asphyxia neonatal because of the predicted results along with recommendations for focusing mothers with the risk of babies born asphyxia to find out possible childbirth places.
导言：大多数围产期窒息是由与分娩无关的情况引起的。当分娩过程中发生窒息时，通常是由孕期未被发现的产科急症引起的。通过确定孕期窒息的发生率来预防窒息至关重要。已有多项研究通过预测模型来识别窒息问题。目的：使用奈伊夫贝叶斯（NB）算法开发一个基于网络的系统，利用基层医疗机构的产前风险因素数据集预测新生儿窒息：本研究采用了研究与开发方法，包括 4 个阶段，即文献研究、开发阶段、专家验证和试用：结果：成功创建了基层医疗机构医务人员可用于预测新生儿窒息并建议转诊以确定分娩地点的系统。系统性能测试预测新生儿窒息的所有 NB 评价值均达到 98% 以上，受访者测试的预测准确率属于高准确率类别（MAPE 值为 9.06%）：使用 NB 算法开发的基于网络的系统已被证明能够预测新生儿窒息，并可为卫生工作者实施，以便根据预测结果预测处理新生儿窒息病例的延误，同时建议有新生儿窒息风险的母亲重点关注可能的分娩地点。
{"title":"Develop a web-based system using the Naïve Bayes algorithm to predict asphyxia neonatal","authors":"Elviga Arselatifa, Sri Sumarni, Kurnianingsih Kurnianingsih","doi":"10.30595/medisains.v22i1.19531","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.19531","url":null,"abstract":"Introduction: Most cases of perinatal asphyxia are caused by conditions unrelated to labor. When asphyxia occurs during childbirth, it is usually caused by an obstetric emergency that was not detected during pregnancy. It is essential to prevent asphyxia by identifying the incidence of asphyxia during pregnancy. Several studies have been conducted to identify asphyxia problems developing by predictive models. However, there has been no development of a system for predicting birth asphyxia during pregnancy and carried out in primary health facilities.Purpose: Develop a web-based system using the Naïve Bayes (NB) algorithm to predict asphyxia neonatal using a dataset of antepartum risk factors in primary health facilities.Methods: This study employed research and development, which consists of 4 stages, namely literature study, development stage, expert validity, and trial.Results: A system that health workers in primary health facilities can use to predict asphyxia neonatal and recommend referrals for determining the place of childbirth has been successfully created. The system performance test predicted asphyxia neonatal with all NB evaluation values reaching more than 98%, and the prediction accuracy in the respondent test included in the High Accuracy category (MAPE value 9.06%).Conclusion: The development of a web-based system using the NB algorithm has been proven to be able to predict asphyxia neonatal and can be implemented for health workers as an effort to anticipate delays in handling cases of asphyxia neonatal because of the predicted results along with recommendations for focusing mothers with the risk of babies born asphyxia to find out possible childbirth places.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141055129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.30595/medisains.v22i1.21720
Roro Kushartanti, Aprin Wahyu Wijayanti
Wolbachia is a bacterium that generally occurs naturally in 50% of insect species, lives in insect cells, and is passed from one generation to the next through insect eggs. Wolbachia in the mosquito's body can inhibit the replication of the Dengue virus or other viruses. Based on the Decree of the Minister of Health of the Republic of Indonesia Number 1341 concerning the Implementation of the Pilot Project for the Implementation of Wolbachia as a Dengue Management Innovation, the application of this technology was carried out in five cities, namely Semarang City, West Jakarta, Bandung City, Kupang City, and Bontang City. The effectiveness of Wolbachia itself has been proven and researched since 2011, when it was conducted in Yogyakarta. As a result, Wolbachia was proven to reduce dengue fever cases by up to 77% and reduce the proportion of hospital admissions by 86%. This method of spreading Wolbachia mosquitoes in several cities will reduce local transmission of infection in those areas. This reduction in local transmission of dengue infection occurs both in the rainy and dry seasons. Scientifically, the success of the Wolbachia method is apparent, but the controversy is due to confused information circulating, giving rise to concern among the public. Vector control using Wolbachia is an alternative disease control method that uses mosquito vectors and is quite effective. However, the application of Wolbachia in the community must be monitored continuously to determine its impact on humans, local mosquitoes, and the ecosystem.
{"title":"The innovation of Wolbachia mosquito technology to control dengue hemorrhagic fever","authors":"Roro Kushartanti, Aprin Wahyu Wijayanti","doi":"10.30595/medisains.v22i1.21720","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.21720","url":null,"abstract":"Wolbachia is a bacterium that generally occurs naturally in 50% of insect species, lives in insect cells, and is passed from one generation to the next through insect eggs. Wolbachia in the mosquito's body can inhibit the replication of the Dengue virus or other viruses. Based on the Decree of the Minister of Health of the Republic of Indonesia Number 1341 concerning the Implementation of the Pilot Project for the Implementation of Wolbachia as a Dengue Management Innovation, the application of this technology was carried out in five cities, namely Semarang City, West Jakarta, Bandung City, Kupang City, and Bontang City. The effectiveness of Wolbachia itself has been proven and researched since 2011, when it was conducted in Yogyakarta. As a result, Wolbachia was proven to reduce dengue fever cases by up to 77% and reduce the proportion of hospital admissions by 86%. This method of spreading Wolbachia mosquitoes in several cities will reduce local transmission of infection in those areas. This reduction in local transmission of dengue infection occurs both in the rainy and dry seasons. Scientifically, the success of the Wolbachia method is apparent, but the controversy is due to confused information circulating, giving rise to concern among the public. Vector control using Wolbachia is an alternative disease control method that uses mosquito vectors and is quite effective. However, the application of Wolbachia in the community must be monitored continuously to determine its impact on humans, local mosquitoes, and the ecosystem.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141056938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.30595/medisains.v22i1.20079
Dina Dewi Sartika Lestari Ismail, Heri Kristianto, Setyoadi Setyoadi, Anissa Maydinah
Background: The celery plant (Apium graveolens L.) is a widely distributed herbal plant globally known for being a significant source of natural active products, including phenolic compounds and antioxidants demonstrating antidiabetic activity. Previous research findings have indicated the impact of celery leaf extract on reducing blood glucose levels. However, up to now, there is a dearth of research investigating the potential effect of celery leaf extract on the regeneration of pancreatic beta cells in mice with diabetes models.Objective: This study aims to determine the effect of the ethanol extract of celery leaves on the number of pancreatic cells in Streptozotocin (STZ)-induced mice.Method: This research method uses a randomized controlled group posttest only design. Experimental animals were divided into five groups, including a group of experimental animals in a healthy condition, the group with hyperglycemia, hyperglycemia group with celery extract at 200 mg/kg BW, hyperglycemia group with celery extract at 400 mg/kg, and hyperglycemia group with extract celery is 800 mg/kg BW. Termination was performed on day 60 after therapy and pancreas hematoxylin and eosin staining. They are counting the number of cells with a photo slide with 400x magnification in 5 fields of view—statistical analysis using One-Way ANOVA.Results: The study revealed that celery extract could increase the number of pancreatic cells compared to the control group (P<0.002). Notably, the highest increase in pancreatic cell count was observed in the group administered with 400 mg/BW of celery extract.Conclusion: Celery leaf extract effectively increased the number of beta cells in vivo.
{"title":"In vivo study of celery (Apium graveolens L) extract for regenerating pancreatic β-cell","authors":"Dina Dewi Sartika Lestari Ismail, Heri Kristianto, Setyoadi Setyoadi, Anissa Maydinah","doi":"10.30595/medisains.v22i1.20079","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.20079","url":null,"abstract":"Background: The celery plant (Apium graveolens L.) is a widely distributed herbal plant globally known for being a significant source of natural active products, including phenolic compounds and antioxidants demonstrating antidiabetic activity. Previous research findings have indicated the impact of celery leaf extract on reducing blood glucose levels. However, up to now, there is a dearth of research investigating the potential effect of celery leaf extract on the regeneration of pancreatic beta cells in mice with diabetes models.Objective: This study aims to determine the effect of the ethanol extract of celery leaves on the number of pancreatic cells in Streptozotocin (STZ)-induced mice.Method: This research method uses a randomized controlled group posttest only design. Experimental animals were divided into five groups, including a group of experimental animals in a healthy condition, the group with hyperglycemia, hyperglycemia group with celery extract at 200 mg/kg BW, hyperglycemia group with celery extract at 400 mg/kg, and hyperglycemia group with extract celery is 800 mg/kg BW. Termination was performed on day 60 after therapy and pancreas hematoxylin and eosin staining. They are counting the number of cells with a photo slide with 400x magnification in 5 fields of view—statistical analysis using One-Way ANOVA.Results: The study revealed that celery extract could increase the number of pancreatic cells compared to the control group (P<0.002). Notably, the highest increase in pancreatic cell count was observed in the group administered with 400 mg/BW of celery extract.Conclusion: Celery leaf extract effectively increased the number of beta cells in vivo.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141029529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Human papillomavirus is an infection that causes malignancy because of persistency and modulation of apoptotic pathways, especially caspase-3. Factors that can increase persistency, recurrency, and malignancy of HPV infection include HIV infection with low CD4 levels. There is disagreement or deep molecular understanding of the induction and modulation of apoptosis in HIV-mediated CD4+ T cell depletion, especially in CA. However, it is necessary to see how CD4+ levels can influence caspase-3, so it may open up new avenues for supporting investigation to consider the presence of malignancy or therapeutic strategies regarding CD4+ can induce apoptosis.Purpose: This study aimed to determine whether there is a correlation between CD4+ levels and caspase-3 expression in condyloma acuminata with HIV reactive patients.Methods: This is an analytic observational study with a cross-sectional design. The research was conducted at Dr. Moewardi General Hospital from August to December 2023. Nineteen patients with condyloma acuminata and HIV reactive were included in this study with a consecutive sampling technique. The expression of caspase-3 was assessed using immunohistochemical staining, looking at the percentage of stained cell nuclei and cytoplasm and CD4+ levels with flow cytometry examination—data analysis using Pearson correlation.Results: Respondents in this study were primarily men, self-employed with heterosexual orientation and genito-genital-oral sexual intercourse. The statistical analysis showed no significant relationship (p: 0.300, r: -0.251) between CD4+ levels and caspase-3 expression.Conclusions: While not statistically significant, CD4+ level is reduced in correlation with increased caspase-3 expressions.
背景：人类乳头瘤病毒是一种可导致恶性肿瘤的感染，因为它具有持续性并可调节细胞凋亡途径，尤其是 Caspase-3。可增加人乳头瘤病毒感染的持续性、复发性和恶性程度的因素包括艾滋病毒感染和低 CD4 水平。对于艾滋病毒介导的 CD4+ T 细胞耗竭（尤其是在 CA 中）中凋亡的诱导和调控，目前尚存在分歧或深刻的分子认识。然而，有必要了解CD4+水平如何影响caspase-3，这样可能会为支持性研究开辟新的途径，以考虑是否存在恶性肿瘤或有关CD4+可诱导细胞凋亡的治疗策略。目的：本研究旨在确定HIV反应性尖锐湿疣患者的CD4+水平与caspase-3表达之间是否存在相关性：这是一项横断面设计的分析性观察研究。研究于 2023 年 8 月至 12 月在莫瓦迪博士综合医院进行。本研究采用连续抽样技术，纳入了 19 名尖锐湿疣和 HIV 反应性患者。采用免疫组化染色法评估 caspase-3 的表达，观察染色细胞核和细胞质的百分比，并采用流式细胞术检查 CD4+ 的水平--采用皮尔逊相关法进行数据分析：本研究的受访者主要为男性，自营职业者，异性恋倾向，有生殖器-生殖器-口交行为。统计分析显示，CD4+水平与 Caspase-3 表达之间无明显关系（P：0.300，r：-0.251）：结论：CD4+水平的降低与caspase-3表达的增加相关，但无统计学意义。
{"title":"Correlation of CD4+ levels and caspase-3 in condyloma acuminata with HIV reactive patients","authors":"Elok Nurfaiqoh, P. Mawardi, Nugrohoaji Dharmawan, Indah Julianto, Endra Yustin Ellistasari","doi":"10.30595/medisains.v22i1.21501","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.21501","url":null,"abstract":"Background: Human papillomavirus is an infection that causes malignancy because of persistency and modulation of apoptotic pathways, especially caspase-3. Factors that can increase persistency, recurrency, and malignancy of HPV infection include HIV infection with low CD4 levels. There is disagreement or deep molecular understanding of the induction and modulation of apoptosis in HIV-mediated CD4+ T cell depletion, especially in CA. However, it is necessary to see how CD4+ levels can influence caspase-3, so it may open up new avenues for supporting investigation to consider the presence of malignancy or therapeutic strategies regarding CD4+ can induce apoptosis.Purpose: This study aimed to determine whether there is a correlation between CD4+ levels and caspase-3 expression in condyloma acuminata with HIV reactive patients.Methods: This is an analytic observational study with a cross-sectional design. The research was conducted at Dr. Moewardi General Hospital from August to December 2023. Nineteen patients with condyloma acuminata and HIV reactive were included in this study with a consecutive sampling technique. The expression of caspase-3 was assessed using immunohistochemical staining, looking at the percentage of stained cell nuclei and cytoplasm and CD4+ levels with flow cytometry examination—data analysis using Pearson correlation.Results: Respondents in this study were primarily men, self-employed with heterosexual orientation and genito-genital-oral sexual intercourse. The statistical analysis showed no significant relationship (p: 0.300, r: -0.251) between CD4+ levels and caspase-3 expression.Conclusions: While not statistically significant, CD4+ level is reduced in correlation with increased caspase-3 expressions.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141039935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.30595/medisains.v22i1.19834
Sudigdo Dwi Prasetyo, Mukhoirotin Mukhoirotin???
Background: Impaired sleep quality is a common problem experienced by nurses working shifts, and if not treated properly, it can impact their work performance and overall health. Spiritual Emotional Freedom Technique (SEFT) therapy has been proven to improve sleep quality. However, its effectiveness has never been studied in nurses who experience sleep disorders due to working shifts.Objective: To investigate the influence of spiritual relaxation therapy on sleep quality among nurses experiencing sleep disturbances due to shift work.Method: This is a Quasi-Experimental study with a pre-test-posttest control group design. A total of 38 nurses who experience sleep disorders due to working shifts were included in this study. The PSQI questionnaire was utilized as the instrument to measure sleep quality. Data analysis involved the Mann-Whitney test.Results: The average sleep quality before the intervention was in the mild category; after the intervention, most were in the good category. The results showed that the sleep quality of nurses who received spiritual relaxation was better than the control group (p<0.001).Conclusion: Spiritual relaxation therapy effectively improves the sleep quality of nurses experiencing sleep disturbances due to shift work.
{"title":"Spiritual relaxation therapy to improve sleep quality for nurses working shifts who experience sleep problems: a quasi-experimental study","authors":"Sudigdo Dwi Prasetyo, Mukhoirotin Mukhoirotin???","doi":"10.30595/medisains.v22i1.19834","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.19834","url":null,"abstract":"Background: Impaired sleep quality is a common problem experienced by nurses working shifts, and if not treated properly, it can impact their work performance and overall health. Spiritual Emotional Freedom Technique (SEFT) therapy has been proven to improve sleep quality. However, its effectiveness has never been studied in nurses who experience sleep disorders due to working shifts.Objective: To investigate the influence of spiritual relaxation therapy on sleep quality among nurses experiencing sleep disturbances due to shift work.Method: This is a Quasi-Experimental study with a pre-test-posttest control group design. A total of 38 nurses who experience sleep disorders due to working shifts were included in this study. The PSQI questionnaire was utilized as the instrument to measure sleep quality. Data analysis involved the Mann-Whitney test.Results: The average sleep quality before the intervention was in the mild category; after the intervention, most were in the good category. The results showed that the sleep quality of nurses who received spiritual relaxation was better than the control group (p<0.001).Conclusion: Spiritual relaxation therapy effectively improves the sleep quality of nurses experiencing sleep disturbances due to shift work.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141051046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Swallow's nest has a glycol content of proteins, carbohydrates (25.62–27.26%), and proteins (62–63%); protein is an indispensable content for the healing process of perineal wounds, protein plays the main ingredient in cell formation, and repairs damaged tissues. In previous studies, swallow's nest extract against perineal wounds in mice obtained the results of accelerated healing on days 3 and 4. However, no studies have been conducted on human perineal injuries.Purpose: Knowing the effectiveness of the swallow's nest extract gel (Aerodramus Fuciphagus) 30% against wound healing and a decrease in the number of colonies of staphylococcus aureus bacteria in the perineal wounds of the postpartum mother.Methods: This experiment study has a post-test with a control group design. The sample comprised 34 postpartum maternal respondents divided into two groups randomly (intervention and control groups). The intervention group was given gel from swallow's nest extract while the control group carried out a dry clean treatment; both were treated to the wound. Perineal wound healing based on REEDA scores was analyzed using the Friedman test, while bacteria in perineal wounds were analyzed using the Man-Whitney test.Results: Based on the REEDA scale, the perineal wound healing rate in the intervention group was better, where healing had already occurred on day 5, compared to the control group that occurred on day 7 (p<0.0001). The number of bacteria in the perineal wounds of the intervention group was less, with an average of 0.06 CFU, compared to the control group's average control group's average of 1.11 CFU (p<0.05).Conclusion: Swallow's nest extract is influential and beneficial in healing perineal wounds in postpartum mothers and has antibacterial activity.
{"title":"Antibacterial activity and perineal wound healing of swallow’s gel extracts","authors":"Gustiyana Astiti, Runjati Runjati, Sudirman Sudirman","doi":"10.30595/medisains.v22i1.15197","DOIUrl":"https://doi.org/10.30595/medisains.v22i1.15197","url":null,"abstract":"Background: Swallow's nest has a glycol content of proteins, carbohydrates (25.62–27.26%), and proteins (62–63%); protein is an indispensable content for the healing process of perineal wounds, protein plays the main ingredient in cell formation, and repairs damaged tissues. In previous studies, swallow's nest extract against perineal wounds in mice obtained the results of accelerated healing on days 3 and 4. However, no studies have been conducted on human perineal injuries.Purpose: Knowing the effectiveness of the swallow's nest extract gel (Aerodramus Fuciphagus) 30% against wound healing and a decrease in the number of colonies of staphylococcus aureus bacteria in the perineal wounds of the postpartum mother.Methods: This experiment study has a post-test with a control group design. The sample comprised 34 postpartum maternal respondents divided into two groups randomly (intervention and control groups). The intervention group was given gel from swallow's nest extract while the control group carried out a dry clean treatment; both were treated to the wound. Perineal wound healing based on REEDA scores was analyzed using the Friedman test, while bacteria in perineal wounds were analyzed using the Man-Whitney test.Results: Based on the REEDA scale, the perineal wound healing rate in the intervention group was better, where healing had already occurred on day 5, compared to the control group that occurred on day 7 (p<0.0001). The number of bacteria in the perineal wounds of the intervention group was less, with an average of 0.06 CFU, compared to the control group's average control group's average of 1.11 CFU (p<0.05).Conclusion: Swallow's nest extract is influential and beneficial in healing perineal wounds in postpartum mothers and has antibacterial activity.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141058397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.30595/medisains.v21i2.17484
Farah Salsabila, S. Hadisaputro, Lanny Sunarjo
Background: As a complication of pregnancy, uncontrolled hypertension will continue to be a complication that harms both mother and baby. Pomegranate peel, which contains high antioxidants, is potentially used as an antihypertensive of adjuvant pharmacological therapy. However, there has been no research on the effect of pomegranate extract in lowering blood pressure, so it is necessary to do related research.Purpose: Analyze the effect of pomegranate peel extract on blood pressure and nitric oxide levels in gestational hypertension.Methods: This is a quasi-experiment design pre-post-test with the control group. Through purposive sampling techniques, 32 respondents were divided into two groups. During 14 days, the intervention group received nifedipine 10 mg/day with 500 mg of pomegranate peel extract, while another group was only given nifedipine 10 mg/day.Results: The blood pressure of the intervention group decreased compared to the control group (p<0.05). Meanwhile, the nitric oxide level of the intervention group is increased compared to the control group (p<0.05).Conclusions: Pomegranate peel extract can reduce blood pressure and enhance nitric oxide levels in gestational hypertension.
{"title":"Effect of pomegranate extract (Punica granatum L.) on blood pressure and nitric oxide in gestational hypertension","authors":"Farah Salsabila, S. Hadisaputro, Lanny Sunarjo","doi":"10.30595/medisains.v21i2.17484","DOIUrl":"https://doi.org/10.30595/medisains.v21i2.17484","url":null,"abstract":"Background: As a complication of pregnancy, uncontrolled hypertension will continue to be a complication that harms both mother and baby. Pomegranate peel, which contains high antioxidants, is potentially used as an antihypertensive of adjuvant pharmacological therapy. However, there has been no research on the effect of pomegranate extract in lowering blood pressure, so it is necessary to do related research.Purpose: Analyze the effect of pomegranate peel extract on blood pressure and nitric oxide levels in gestational hypertension.Methods: This is a quasi-experiment design pre-post-test with the control group. Through purposive sampling techniques, 32 respondents were divided into two groups. During 14 days, the intervention group received nifedipine 10 mg/day with 500 mg of pomegranate peel extract, while another group was only given nifedipine 10 mg/day.Results: The blood pressure of the intervention group decreased compared to the control group (p<0.05). Meanwhile, the nitric oxide level of the intervention group is increased compared to the control group (p<0.05).Conclusions: Pomegranate peel extract can reduce blood pressure and enhance nitric oxide levels in gestational hypertension.","PeriodicalId":52913,"journal":{"name":"Medisains","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46085168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：