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EFFECT OF VIRGIN OLIVE OIL SUPPLEMENTATION ON LIPID PROFILE AND OXIDATIVE STATUS IN RATS 补充初榨橄榄油对大鼠血脂和氧化状态的影响
Pub Date : 2019-06-01 DOI: 10.21608/JVMR.2019.43331
A. Farahat, A. SawiressF., A. AghwiderA.
The aim of the present study is to investigate the effect of virgin olive oil on some blood parameters in male Albino rats supplemented with normal diet. thirty male Sprague Dawley rats, (90-110 g), were used in the present study, and were divided into three groups (10 in each), 1st group (control), received basal diet and supplemented with 1ml saline. 2nd and 3rd groups received basal diet, and supplemented daily with 1ml/100 gm B.W and 2ml/100 gm B.W of virgin olive oil (VOO), respectively for 4 weeks. Blood samples were collected weekly from all rats. Serum samples was obtained for assay of lipid profile levels and hepatic lipid peroxidation (MDA) enzyme. Blood lysate was used for antioxidant enzymes activities SOD, GPx and CAT.
本研究旨在探讨初榨橄榄油在正常饮食的基础上对雄性白化大鼠某些血液指标的影响。实验选用雄性Sprague Dawley大鼠30只,体重90 ~ 110 g,分为3组,每组10只,第一组为对照组,饲喂基础日粮,并添加生理盐水1ml。第2组和第3组饲喂基础饲粮,每天分别添加1ml/100 gm B.W和2ml/100 gm B.W的初榨橄榄油(VOO),连续4周。每周采集所有大鼠的血样。取血清样本,测定血脂水平和肝脂质过氧化(MDA)酶。血溶液测定抗氧化酶SOD、GPx和CAT活性。
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引用次数: 1
A study on the effect of age, breed and season on sexual behaviour, testosterone concentrations and scrotal circumference in Egyptian rams 年龄、品种和季节对埃及公羊性行为、睾酮浓度和阴囊周长影响的研究
Pub Date : 2019-06-01 DOI: 10.21608/JVMR.2019.43340
Ahmad S. Mostafa, Mostafa Farghal
h: 0px; "> The objective of this study was to detect the influence of age, breed and season on sexual behaviour, testosterone concentrations and scrotal circumference in Egyptian rams. The study was carried out at agricultural research center belonging to Minia University, Shosha village, Samalut city, El-Minia. Ten crossbred and five adult Ossimi rams were used during this study. Every ram was allowed to breed two ewes to evaluate its sexual behaviour for a twenty minutes test. Blood samples were collected three times per season at two weeks interval from each ram during the period of experiment to measure testosterone concentrations. Also, scrotal circumference was measured at the same frequency and time interval as blood samples. Sexual behaviour was better in old rams than young ones (p 0.05). Serum testosterone level was higher at autumn (7.13±02.9 ng/ml) than at winter (5.54±0.29 ng/ml) in crossbred rams (p<0.01), while in Ossimi rams, higher level was obtained in spring (8.24±.39 ng/ml) than in summer (3.99±0.40 ng/ml). Mean scrotal circumference was higher in older rams (30.20±1.20 cm) than younger rams (22.53±1.27 cm). There was no significant difference for measures of scrotal circumference between crossbred rams (30.86±1.09 cm) and Ossimi rams (30.93±1.06 cm). Season had no significant effect on scrotal circumference in crossbred rams, whereas in Ossimi rams the effect of season was significantly obvious with the highest and lowest values were recorded in spring (31.0±1.05 cm) and winter (30.60±1.17 cm) respectively. It could be concluded from this study that the age of rams had a great influence on their sexual behaviour and scrotal circumference, while the effect of breed and season on sexual behaviour and scrotal circumference was not clear.
h: 0 px;这项研究的目的是检测年龄、品种和季节对埃及公羊的性行为、睾酮浓度和阴囊周长的影响。该研究是在El-Minia Samalut市Shosha村Minia大学农业研究中心进行的。本研究选用杂交公羊10只,成年公羊5只。每只公羊被允许繁殖两只母羊,在20分钟的测试中评估其性行为。在实验期间,每只公羊每季度每隔两周采集三次血液样本以测定睾酮浓度。同时,阴囊周长以与血液样本相同的频率和时间间隔测量。老年公羊的性行为优于年轻公羊(p < 0.05)。杂交公羊秋季血清睾酮水平(7.13±02.9 ng/ml)高于冬季血清睾酮水平(5.54±0.29 ng/ml) (p<0.01),而欧西米公羊春季血清睾酮水平(8.24±0.29 ng/ml)高于冬季(p<0.01)。(3.99±0.40)ng/ml。年龄较大的公羊阴囊平均周长(30.20±1.20 cm)高于年龄较小的公羊(22.53±1.27 cm)。杂交公羊的阴囊周长(30.86±1.09 cm)与奥西米公羊(30.93±1.06 cm)无显著差异。季节对杂交公羊阴囊周长的影响不显著,而季节对奥西米公羊阴囊周长的影响显著,春季最高(31.0±1.05 cm),冬季最低(30.60±1.17 cm)。结果表明,公羊的年龄对其性行为和阴囊周长有较大的影响,而品种和季节对其性行为和阴囊周长的影响不明显。
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引用次数: 2
Normal cross sectional anatomy and magnetic resonance imaging of pastern and coffin joints in camel 骆驼关节和棺材关节的正常横断解剖和磁共振成像
Pub Date : 2019-01-01 DOI: 10.21608/jvmr.2019.44150
Azza A. H. Ibrahim, Zein El-Abdein A. Adam, Mohamed G. Tawfiek
ARTICLE INFO The present study aimed to describe the normal cross sectional anatomy and magnetic resonance imaging of pastern and coffin joints in dromedary camel. This study was conducted on twelve distal limbs (fore and hind) of fresh cadavers from three adult camels of both sexes. The specimens appeared normal without orthopedic disorders. Twelve distal limbs were scanned using a 1 Tesla MRI scanner and then injected with colored latex to be sectioned into sagittal, dorsal and transverse slices. Cross anatomical sections were matched with their corresponding MR images for identification and evaluation of the clinically relevant anatomical structures that appeared with different signal intensities on MRI scans. The present study showed that all major soft tissues in pastern and coffin joints of camel were clearly depicted on MR images, however, the palmar/plantar ligaments of pastern joint and ligaments of navicular cartilage could not be identified on MR images. The annotated cross anatomical sections with the corresponding MR images could be used as a normal reference for interpretation of some clinical diseases in pastern and coffin joints of camel. Article history:
摘要本研究旨在描述单峰骆驼关节和棺关节的正常横切面解剖和磁共振成像。本研究对3只雌雄骆驼的新鲜尸体的12个远端肢体(前后)进行了研究。标本外观正常,无矫形障碍。采用1特斯拉MRI扫描仪对12条远端肢体进行扫描,然后注射彩色乳胶切片,分为矢状、背侧和横切面。将交叉解剖切片与其对应的MR图像进行匹配,以识别和评估MRI扫描中出现的不同信号强度的临床相关解剖结构。本研究表明,在MR图像上可以清晰地描绘出骆驼的所有主要的关节和骨关节软组织,但在MR图像上无法识别出关节掌/足底韧带和舟状软骨韧带。经注释的交叉解剖切片和相应的MR图像可作为解释骆驼关节和棺材关节某些临床疾病的正常参考。文章历史:
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引用次数: 3
Cross sectional anatomy, magnetic resonance imaging and computed tomography of fetlock joint in camel 骆驼飞蹄关节的横断解剖、磁共振成像和计算机断层扫描
Pub Date : 2019-01-01 DOI: 10.21608/jvmr.2019.44147
Azza A. H. Ibrahim, Zein El-Abdein A. Adam, Mohamed G. Tawfiek
ARTICLE INFO The current study aimed to describe the normal cross-sectional anatomy, magnetic resonance imaging, and computed tomography of the fetlock joint in adult healthy dromedary camel. This study was carried out on twelve fetlock joints of the fresh cadavers from three camels. The gross examination of these camels revealed that they were normal with no orthopedic abnormalities. The cadaveric fetlock joints (n=12) were scanned using a computed tomographic (CT) scanner and a 1 Tesla MRI scanner. Then the joints were injected with a colored latex and sectioned into transverse, dorsal, and sagittal slices. Crossanatomical sections were correlated with their corresponding CT and MR images for evaluation of the normal relevant anatomical structures that appeared with different signal intensities on the CT and MRI scans. The current investigation revealed that all the major soft tissue structures in the fetlock joint of the dromedary camel were clearly visualized on both CT and MR scans, except the short and the cruciate sesamoidean ligaments that could not be identified on both the CT and MR images. The anatomical sections with their corresponding CT and MR images obtained in this study could be used as a reference for subsequent clinical diagnosis and the interpretation of fetlock joint pathologies in dromedary camel. Received: 29/04/ 2019 Accepted: 30/07/2019 Published online: 02/08/2019
本研究旨在描述成年健康单峰骆驼的正常横截面解剖、磁共振成像和计算机断层扫描。本研究对3头骆驼新鲜尸体的12个爪关节进行了研究。这些骆驼的大体检查显示它们是正常的,没有任何骨科异常。使用计算机断层扫描(CT)扫描仪和1特斯拉MRI扫描仪扫描尸体锁关节(n=12)。然后用彩色乳胶注射关节,切成横、背、矢状面。交叉解剖切片与相应的CT和MR图像相关联,以评估CT和MRI扫描中出现不同信号强度的正常相关解剖结构。本研究结果显示,除短韧带和十字韧带在CT和MR上不能识别外,单峰骆驼锁关节的所有主要软组织结构在CT和MR上都清晰可见。本研究获得的解剖切片及其相应的CT和MR图像可作为后续临床诊断的参考,并可作为单峰骆驼锁肢关节病理解释的参考。收稿日期:29/04/ 2019收稿日期:30/07/2019发布日期:02/08/2019
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引用次数: 4
Prevalence of Salmonella Spp in Some broiler farmsin different Egyptian Governorates 沙门氏菌在埃及不同省份一些肉鸡养殖场的流行情况
Pub Date : 2018-12-01 DOI: 10.21608/JVMR.2017.43315
H. Kaoud, M. A. El-Babbly, K. G. El-Iraqi, M. Khalil
This study was conducted to evaluatethe degree of biosecurity level with especial reference to Salmonella Spp as an example to explain the expected causes and risk factors that leads to spread them in poultry flocks in Egypt then studied farms evaluated for the sensitivity of Salmonella isolates to the most common disinfectants used in Egypt.About 300 samples (100 cloacal swabs, 100 liver and intestinal samples,100 litter samples) were collected from 10 broiler farms with different age (at 0 old day, one week,2,4 and 6 weeks of age)then the samples were investigated for Salmonella Sppand subsequently identified based on biochemical and serological tests.The obtained results showed that 35 Salmonella species were isolated from 10 broiler poultry houses (25%);(6%) and (4%) from cloacal swab;liver and litter, respectively. Average prevalence of Salmonella spp. was 11.33 % in open broiler houses whether raised Cobb, Ross or Sasso breeds. Salmonella Typhimurium, S. enteritidisand S. Kentuckywerethe most serovars out of the 35 detected isolates.There was great statistical significant difference in the sensitivity of Salmonella isolates to the most common disinfectants(P<0.05)asVerkonS ® achieved 3 log reduction , after 5-minute,Formalin and Phenique were achieved 3 log reductions against S. entriditisafter one-minute Aldekol DesGda ® achieved one log reduction after one minute, TH4 ® ,Biosentry ® 904 and Iodophoreachieved 2 log reductions after 5 minutes. *Corresponding author. Department of Veterinary Hygiene, Faculty of Veterinary Medicine, Beni Seuif University. Tel: 01224688745 ARTICLE INFO
本研究以埃及沙门氏菌为例,评价其生物安全水平,解释导致其在家禽群中传播的预期原因和危险因素,并研究了埃及农场沙门氏菌分离株对最常用消毒剂的敏感性。从10个不同日龄(0日龄、1周龄、2周龄、4周龄和6周龄)的肉鸡养殖场采集约300份样本(100份粪签、100份肝脏和肠道样本、100份窝产仔样本),对样本进行沙门氏菌检测,并进行生化和血清学检测。结果表明,从10个肉鸡鸡舍中分离出35种沙门氏菌,分别占25%、6%和4%。露天鸡舍中沙门氏菌的平均流行率为11.33%,无论饲养的是科布、罗斯还是萨索种鸡。在检测到的35株分离株中,鼠伤寒沙门氏菌、肠炎沙门氏菌和肯塔基沙门氏菌的血清型最多。沙门氏菌分离物对最常见消毒剂的敏感性差异有统计学意义(P<0.05), verkons®在5分钟后降低了3个对数,福尔玛和Phenique在1分钟后降低了3个对数,dekol DesGda®在1分钟后降低了1个对数,TH4®、Biosentry®904和碘磷在5分钟后降低了2个对数。*通讯作者。贝尼苏义夫大学兽医学院兽医卫生系。联系电话:01224688745
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引用次数: 3
Antioxidant, immunostimulant and renal protective activities of tri-herbal combination in African Sharptooth Catfish, Clarias gariepinus 三药组合对非洲尖牙鲶鱼抗氧化、免疫刺激和肾保护作用的影响
Pub Date : 2018-12-01 DOI: 10.21608/JVMR.2017.43320
W. Emeish, Z. Al-Amgad, H. Ahmed
Medicinal herbal feed that used as feed additives are widely applied in livestock production and may be applicable to aquaculture production systems. The present study explores the modulatory effects of incorporation of herbal combination of black cumin seeds (Nigella sativa), peppermint (Mentha piperita) and fennel (Foeniculum vulgare), on African Sharptooth Catfish, Clarias gariepinus. For this purpose, a total of 72 catfish were randomly distributed into 6 groups of 12 fish each and fed on the experimental diets for 45 successive days. Fish of first group were fed on basic diet only while, those of the second one were fed on basic diet supplemented with 0.5% from each medicinal plant while, other 4 groups were subjected to challenge experiment by injection with Vibrio vulnificus. The third group was kept non-injected, fourth group was injected by saline, fifth and sixth groups were injected by Vibrio vulnificus after feeding with basic diet and basic diet with herbs, respectively. Results proved that, total antioxidant capacity, serum total proteins, albumins and globulins were observed to be significantly higher in the treated group as compared to the control. Lymphocytes percent increased significantly in herbal fed group comparing to the control group while, neutrophils percent decreased significantly. Although serum urea level was not affected by herbal supplement, serum creatinine level was decreased significantly. Body weight gain of C. gariepinus increased significantly after herbal administration. Catfish challenged with a Vibrio vulnificus isolate and received the herbal diet showed less mortality than the control group. Fish fed on the herbal diet exhibited normal histological structure of liver, kidney and spleen. In conclusion, based on the current results together with the low cost and the potential antioxidant and immune effects of mixed herbal medicinal plants used in the study, it is recommended to be used in fish feed to diminish the mortalities caused by some aquatic pathogens. ARTICLE INFO
作为饲料添加剂的中草药饲料在畜牧生产中应用广泛,可适用于水产养殖生产系统。本研究探讨了黑孜然种子(Nigella sativa)、薄荷(Mentha piperita)和茴香(Foeniculum vulgare)中药组合对非洲尖齿鲶鱼Clarias gariepinus的调节作用。试验将72尾鲶鱼随机分为6组,每组12尾,连续投喂45 d。第一组只饲喂基础饲料,第二组饲喂在基础饲料中添加0.5%每种药用植物的饲料,其余4组进行创伤弧菌注射攻毒试验。第3组不注射,第4组注射生理盐水,第5组和第6组分别饲喂基础日粮和基础日粮加草药后注射创伤弧菌。结果表明,治疗组的总抗氧化能力、血清总蛋白、白蛋白和球蛋白均显著高于对照组。与对照组相比,草药饲料组淋巴细胞百分比显著升高,中性粒细胞百分比显著降低。添加草药对血清尿素水平无影响,但血清肌酐水平显著降低。给药后鸡体增重显著增加。用创伤弧菌分离菌攻毒并食用草药饲料的鲶鱼死亡率低于对照组。饲喂草药饲料的鱼肝脏、肾脏和脾脏组织结构正常。综上所述,根据目前的研究结果,再加上本研究中使用的混合中草药成本低,具有潜在的抗氧化和免疫作用,建议将其用于鱼类饲料中,以降低某些水生病原体造成的死亡率。条信息
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引用次数: 2
Prenatal development of submandibular salivary gland of New-Zealand rabbits 新西兰兔下颌下唾液腺的产前发育
Pub Date : 2018-12-01 DOI: 10.21608/jvmr.2017.43325
S. M. Soliman, Khalid M.Mazher, T. M. Nabil, Shimaa M. Essam
ARTICLE INFO The paired submandibular gland plays a critical role in maintaining normal oral biology through the lubricating and antimicrobial actions of produced saliva. In this study, the developmental stages of the submandibular salivary gland of the New Zealand rabbits were investigated. Twenty New-Zealand rabbit fetuses of 11-30 days old were used. Samples of the head region and submandibular salivary glands were histologically stained and examined under a light microscope. Results revealed that the submandibular primordia appeared as bilateral invaginated epithelial buds from the linguo-gingival groove on the 12th day of prenatal life. By the 15 day, the buds deeply grow to form a cord-like structure that ends with compact bulges forming the future primitive acini. The formed cords are then branching to develop the primary glandular ducts by the 17 day, which are canalized on the 18 day. The primitive gland capsule was observed by the 22 day. The lobulation was recognized and became well developed by the 25 day. Similetanouly, the glandular duct system is completely developed, and serous adenomeres fully occupied the parenchyma and are surrounded by myoepithelial cells. The full-term fetuses have fully developed submandibular glands with a typically compound tubulo-acinar nature and parenchymal seromucoid adenomeres. The PAS staining revealed a strong positive reaction in the striated ducts; however, weak PAS reactions were noticed in the cytoplasm of the acinar cells Received 11/2018 Accepted 12/2018 Online 12/ 2018
配对的下颌腺通过分泌唾液的润滑和抗菌作用,在维持正常口腔生物学中起关键作用。本研究对新西兰兔下颌下唾液腺的发育阶段进行了研究。选用11 ~ 30日龄新西兰兔胎20只。样品的头部区域和下颌下唾液腺进行组织学染色,并在光镜下检查。结果显示,在产前第12天,下颌下原基以双侧舌龈沟内陷上皮芽的形式出现。到第15天,芽深入生长,形成绳状结构,最后形成致密的凸起,形成未来的原始腺泡。形成的腺索在第17天开始分支形成初级腺管,在第18天形成管道。第22天观察原始腺囊。分叶在第25天得到确认并发育良好。同样,腺管系统发育完全,浆液性腺粒完全占据实质并被肌上皮细胞包围。足月胎儿已发育完全的下颌腺,具有典型的复合小管-腺泡性质和实质浆液样腺粒。PAS染色显示条纹管有强烈的阳性反应;然而,在腺泡细胞的细胞质中发现了微弱的PAS反应
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引用次数: 0
Effects of some dietary supplements on the reproductive and productive performances in male rats. 一些膳食补充剂对雄性大鼠生殖和生产性能的影响。
Pub Date : 2018-12-01 DOI: 10.21608/JVMR.2017.43319
A. Aboul-Ela, Eid Abdel-Hamid Mabrouk, N. Helmy, S. Mohamed
The present study aimed to investigate the efficacy of three natural food supplements (water hyacinth [Eichhorniacrassipes, ''EC''], Yeast [Saccharomyces cerevisiae, ''S. cerevisiae''] and date seeds) on the reproductive and productive activities in male rats. Thus, 40 male albino rats were used and divided equally into 4 groups; Control group (fed normal basal diet), EC supplemented group (400 mg EC / kg body weight), S. cerevisiae supplemented group (120 mg / kg body weight) and date seed supplemented group (0.2 mg / kg body weight). Two months later, all rats were sacrificed and all samples were collected. Results revealed that date seeds supplementation increased significantly the body weight gain. Moreover, date seeds and S. cerevisiae supplementation increased significantly gonadosomatic index, serum levels of total antioxidant capacity decreased serum level of malondialdehyde ''MDA''. On the other side, EC supplementation reduced significantly the studied reproductive parameters as well as it decreased the serum level of TAC and increased the level of MDA. Histopathologicaly, seminiferous tubules appeared with huge amount of spermatids in date seeds group and with moderate number of spermatids in S. cerevisiae group and with few number of spermatids in EC group. Therefore, the present study highly recommends the usage of Saccharomyces cerevisiae as well as date seeds to minimize the ration costs, get the optimal benefit from the natural components of both supplements as well as to induce a higher productive and reproductive performance among animals. ARTICLE INFO
本研究旨在探讨水葫芦(Eichhorniacrassipes,“EC”)、酵母(Saccharomyces cerevisiae,“S. cerevisiae”)和枣籽三种天然食品补充剂对雄性大鼠生殖和生产能力的影响。选用雄性白化大鼠40只,平均分为4组;对照组(饲喂正常基础饲粮)、EC添加组(EC / kg体重400 mg)、酵母添加组(EC / kg体重120 mg)和枣籽添加组(0.2 mg / kg体重)。2个月后处死所有大鼠,收集所有样本。结果表明,添加枣子显著增加了体重。此外,添加红枣籽和酵母显著提高了血清促性腺指数,降低了血清丙二醛(MDA)水平。另一方面,添加EC显著降低了所研究的生殖参数,降低了血清TAC水平,增加了MDA水平。组织病理学观察显示,枣籽组精细胞数量巨大,酿酒酵母组精细胞数量适中,EC组精细胞数量较少。因此,本研究强烈建议使用酿酒酵母和枣籽,以最大限度地降低日粮成本,从这两种补充剂的天然成分中获得最佳效益,并提高动物的生产和繁殖性能。条信息
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引用次数: 2
Nosocomial Antiseptics And Disinfectants Resistant Bacteria: microbiological and histopathological studies 院内抗菌剂和消毒剂耐药菌:微生物学和组织病理学研究
Pub Date : 2018-12-01 DOI: 10.21608/jvmr.2017.43326
M. Ahmad, Medhat Abdel Fatath, Khalid A. El-Nesr, Mohamed Ramadan
ARTICLE INFO This study contains evidence-based recommendations for the prevention of hospital acquired infections. Hospital acquired infections are a major cause of mortality and morbidity and provide challenge to clinicians. Measures of infection control included identifying patients at risk of nosocomial infections. Antiseptics and disinfectants are used extensively in hospitals and other health care settings for a variety of topical and hard-surface applications. In particular, they are an essential part of infection control practices and aid in the prevention of nosocomial infection. This study investigated the nosocomial infection via isolation and identification of bacterial pathogens, the effect of detergents against isolated bacteria, and describes the histopathological tissue changes induced by the isolated bacteria in vivo study. Some bacteria resistant to disinfectants were prevalent and these bacteria had different effects on the human, Different pathological changes were seen experimentally by inoculation of these bacteria in mice. Article history: Received 11/2018 Accepted 12/2018 Online 12/2018
文章信息本研究包含预防医院获得性感染的循证建议。医院获得性感染是死亡率和发病率的主要原因,对临床医生提出了挑战。感染控制措施包括确定有院内感染风险的患者。防腐剂和消毒剂广泛用于医院和其他卫生保健机构的各种局部和硬表面应用。特别是,它们是感染控制实践的重要组成部分,有助于预防医院感染。本研究通过对病原菌的分离鉴定,探讨了医院感染情况,探讨了洗涤剂对分离细菌的作用,并描述了分离细菌在体内引起的组织病理变化。一些对消毒剂耐药的细菌普遍存在,这些细菌对人体的影响不同,在小鼠身上接种这些细菌可以观察到不同的病理变化。文章历史:收稿日期:11/2018收稿日期:12/2018上线日期:12/2018
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引用次数: 0
Bacteriological studies on bacterial pathogens isolated from broiler chickens with swollen head syndrome 肉鸡头肿综合征病原菌的细菌学研究
Pub Date : 2018-12-01 DOI: 10.21608/jvmr.2017.43318
Ismail Abd El-Hafeez Radwan, Mohamed Fathy Mohamed, Aya Kamal Eldin Ahmed
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引用次数: 2
期刊
Journal of Veterinary Medical Research
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