Journal of Genetic Engineering and Biotechnology最新文献_第10页

Loss-of-function variant of SLC30A8 rs13266634 (C > T) protects against type 2 diabetes by stabilizing ZnT8: Insights from epidemiological and computational analyses SLC30A8 rs13266634 （C b> T）功能缺失变体通过稳定ZnT8来预防2型糖尿病：来自流行病学和计算分析的见解

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-09-13 DOI: 10.1016/j.jgeb.2025.100565

Nilima Barman , M Atiqul Haque , Md Ridwan , Debabrata Ghosh , Abul B.M.M.K. Islam

The loss-of-function variant rs13266634 (C > T) in SLC30A8 has been linked to type-2-diabetes (T2D) susceptibility with conflicting findings. We investigated its role among T2D patients of Bangladesh. The rs13266634 (C > T) variant results in an arginine-to-tryptophan (p.R325W) substitution of SLC30A8-encoded zinc transporter protein, ZnT8. As zinc is crucial for insulin-crystallization for storage, we analyzed its wild-type (WZnT8) and variant (MZnT8) protein conformations via molecular-dynamics (MD) simulations. For the epidemiological analysis, a case-control study was conducted with 90 T2D patients from a diabetic-patient-cohort and 90 gender-matched healthy-controls. Genomic DNA was isolated from blood samples and genotyped by Sanger sequencing. MD simulations, spanning over 100 ns, were executed using GROMACS v2024.4. The homozygous wild CC-genotype was significantly more frequent in T2D patients (66.7 %) than in control (50.0 %) and was increasing T2D risk, with adjusted odds-ratio (OR_adj) of 2.25 under dominant model. Conversely, the polymorphic TT-genotype showed a protective effect, with an OR_adj of 0.196 (95 % CI:0.045–0.850, p = 0.029) in an additive model. MD simulations revealed that MZnT8 variant exhibited higher structural stability, lower flexibility and a more compact conformation than WZnT8. In conclusion, the polymorphic TT-genotype of SLC30A8 plays a protective role in T2D susceptibility. Its protein variant, MZnT8, adopts a stable, compact conformation with lower flexibility, which may decrease zinc transport in insulin secreting granules and impair insulin crystallization. This structural adaptation could enhance glucose-stimulated insulin secretion and ultimately contribute to T2D protection.

SLC30A8中的功能缺失变体rs13266634 （C >； T）与2型糖尿病（T2D）易感性有关，但研究结果相互矛盾。我们调查了它在孟加拉国t2dm患者中的作用。rs13266634 （C >； T）变异导致slc30a8编码的锌转运蛋白ZnT8的精氨酸到色氨酸（p.R325W）替代。由于锌对胰岛素结晶储存至关重要，我们通过分子动力学（MD）模拟分析了其野生型（WZnT8）和变异型（MZnT8）的蛋白质构象。为了进行流行病学分析，对来自糖尿病患者队列的90例T2D患者和90例性别匹配的健康对照组进行了病例对照研究。从血液样本中分离基因组DNA，并通过桑格测序进行基因分型。使用GROMACS v2024.4执行了超过100 ns的MD模拟。纯合子野生cc基因型在T2D患者中的发生率（66.7%）明显高于对照组（50.0%），并增加了T2D的风险，优势模型下校正比值比（ORadj）为2.25。相反，在加性模型中，多态性tt基因型表现出保护作用，ORadj为0.196 （95% CI: 0.045-0.850, p = 0.029）。MD模拟结果表明，MZnT8变体比WZnT8具有更高的结构稳定性，更低的柔韧性和更紧凑的构象。综上所述，SLC30A8多态性tt基因型对T2D易感性具有保护作用。其蛋白变体MZnT8采用稳定致密的构象，柔韧性较低，可能减少锌在胰岛素分泌颗粒中的运输，损害胰岛素结晶。这种结构适应可以增强葡萄糖刺激的胰岛素分泌，最终有助于T2D保护。

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引用次数: 0

Phenotyping analysis of rice cv. Nipponbare overexpressing OsMADS56 gene in drought tolerance, plant height, and yield components 水稻变异基因表型分析。OsMADS56基因在耐旱性、株高和产量成分中的过表达

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-09-11 DOI: 10.1016/j.jgeb.2025.100570

Dini Nurdiani, Dwi Widyajayantie, Enung Sri Mulyaningsih, Amy Estiati, Satya Nugroho

Background

The MADS-box protein family is a transcription factor (TF) family whose members harbour a MADS-box domain located in their N-terminal. This domain consists of 60 amino acids and functions in DNA binding. In plants, these family genes broadly regulate plant growth, development and responses to various environmental stresses. In this study, an OsMADS56 (702 bp) was isolated from rice (Oryza sativa cv. Nipponbare) to investigate its role in response to drought stress and other agronomic phenotypes at both the vegetative and generative stages.

Results

Rice mutants harbouring overexpressed OsMADS56 gene driven by the constitutive 35S promoter were generated. Quantitative real-time (qRT)-PCR confirmed the overexpression of OsMADS56 at T1 (M76.7) generation. Observation of the T2 OsMADS56-overexpressed rice line (OX7) showed improved tolerance to drought stress. It also showed different agronomic phenotypes compared to wild type (WT), such as shorter plant height (PH) and improved productivity as indicated by earlier heading date (HD), a higher number of panicles (NP), a higher number of grains (NG) and higher grain yield (GY) than WT.

Conclusion

The findings suggest that OsMADS56 was implicated in the rice response to PEG-induced drought stress, rice plant height, and yield components. The OsMADS56 gene and the characterized line can be utilized in further studies on rice adaptation against abiotic stress and its productivity.

MADS-box蛋白家族是一个转录因子（TF）家族，其成员在其n端含有MADS-box结构域。该结构域由60个氨基酸组成，在DNA结合中起作用。在植物中，这些家族基因广泛调控植物的生长、发育和对各种环境胁迫的反应。本研究从水稻（Oryza sativa cv.）中分离到OsMADS56基因（702 bp）。Nipponbare)研究其在营养和生殖阶段对干旱胁迫和其他农艺表型的响应中的作用。结果在组成型35S启动子驱动下获得了OsMADS56基因过表达的水稻突变体。qRT -PCR证实OsMADS56在T1 （M76.7）代过表达。T2 osmads56过表达水稻品系（OX7）对干旱胁迫的耐受性提高。与野生型（WT）相比，OsMADS56表现出不同的农学表型，如较短的株高（PH）、较早的抽穗期（HD）、较高的穗数（NP）、较高的粒数（NG）和较高的产量（GY）。结论OsMADS56参与了水稻对peg诱导的干旱胁迫、水稻株高和产量的响应。OsMADS56基因及其鉴定品系可用于水稻对非生物胁迫的适应性及其产量的进一步研究。

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引用次数: 0

Enzymatic characterization and proteomic profiling of venoms from the medically important Androctonus species 从医学上重要的雄蛾物种毒液的酶特性和蛋白质组学分析

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-09-10 DOI: 10.1016/j.jgeb.2025.100566

Alhussin M.A. Megaly , Masahiro Miyashita , Abdulaziz R. Alqahtani , Mohammed Abdel-Wahab

Egyptian scorpions of the Androctonus genus (family Buthidae) produce life-threatening stings owing to their neurotoxic venom. However, the composition and enzymatic activities of their venoms remain poorly understood: We used electrophoresis to analyze the protein components of venoms collected from three Androctonus species: Androctonus amoreuxi, Androctonus australis, and Androctonus bicolor. Mass spectrometric analysis was performed to characterize the peptides present in these venoms. The phospholipase A₂ (PLA₂), hyaluronidase, and protease activities of the venoms were examined to gauge their potential contribution to venom toxicity. Finally, the antibacterial and hemolytic activities of the venoms were evaluated. The electrophoretic profiles of the three venoms showed features specific to each species, with distinct protein bands observed at 75, 74, 67, 48, 46, 40, and 28 kDa, along with a notable band above the 15-kDa mark. Liquid chromatography/mass spectrometry analyses were used to detect the presence of 369, 324, and 351 components in with molecular masses in the range of 500–10,000 Da in the venoms of A. amoreuxi, A. australis, and A. bicolor, respectively. Disulfide-rich peptides (three disulfide bridges) were abundant, but peptides without disulfide bonds were also detected in all venom samples. All three venoms exhibited hyaluronidase activities, whereas protease and PLA₂ activities were either weak (at 1 µg and 10 µg) or undetectable, even at higher concentrations (up to 20 µg). All assays were performed using venoms standardized by dry weight to ensure consistent protein quantities. Crude venoms of A. amoreuxi and A. australis showed antibacterial activity against E. coli and B. subtilis (5–10 μg), whereas A. bicolor required 10 μg. Hydrophobic fractions (40–55 min) of A. australis alone retained this activity. This work furthers our knowledge of the enzymatic and peptide composition of Androctonus venoms, unveiling their potential in drug delivery enhancement and other biomedical applications. These findings will inform the development of better strategies for the treatment and prevention of scorpion envenomation.

埃及蝎属（蝎科）由于其神经毒性毒液产生危及生命的刺。然而，其毒液的组成和酶活性仍然知之甚少：我们使用电泳分析了三种雄蛾的毒液的蛋白质成分：amoreuxi雄蛾，australis雄蛾和双色雄蛾。质谱分析进行表征肽存在于这些毒液。检测了毒液的磷脂酶A2 （PLA2）、透明质酸酶和蛋白酶活性，以评估它们对毒液毒性的潜在贡献。最后，对毒液的抗菌和溶血活性进行了评价。三种毒液的电泳图谱显示出每个物种特有的特征，在75、74、67、48、46、40和28 kDa处观察到不同的蛋白质条带，并在15 kDa标记以上观察到显著的条带。采用液相色谱/质谱分析方法，分别检测到amoreuxi、A. australis和A. bicolor毒液中存在369、324和351种分子质量在500 ~ 10000 Da范围内的成分。在所有毒液样品中均检测到丰富的二硫肽（3个二硫桥），但也检测到不含二硫键的肽。所有三种毒液都显示出透明质酸酶活性，而蛋白酶和PLA2活性要么很弱（在1µg和10µg时），要么无法检测到，即使在较高浓度（高达20µg）下也是如此。所有检测均使用按干重标准化的毒液进行，以确保蛋白质数量一致。粗毒液对大肠杆菌和枯草芽孢杆菌的抑菌活性为5 ~ 10 μg，对双色拟南芥的抑菌活性为10 μg。疏水部分（40-55 min）单独的南胡麻保持这种活性。这项工作进一步加深了我们对雄蛾毒液的酶和肽组成的了解，揭示了它们在药物输送增强和其他生物医学应用方面的潜力。这些发现将为蝎子中毒的治疗和预防提供更好的策略。

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引用次数: 0

Decoding the cannabis tissue culture puzzle: Machine learning analysis of cannabis in vitro morpho-physiological disorders expands the potential for precision micropropagation 破解大麻组织培养之谜：大麻体外形态生理障碍的机器学习分析扩大了精确微繁殖的潜力

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-09-10 DOI: 10.1016/j.jgeb.2025.100569

Marco Pepe, Mohsen Hesami, Kristian Adamek, Andrew Maxwell Phineas Jones

Cannabis sativa L. (cannabis) has recently re-emerged as an economically important crop, fueling research focused on enhancing production practices to meet market demands. The developing cannabis industry can be improved by overcoming certain production hurdles using micropropagation to maintain and multiply pathogen-free plants in confined spaces at high volumes. However, developing efficient micropropagation systems for cannabis have been hampered by the prevalence of various morpho-physiological disorders, resulting in low multiplication rates, culture decline, and overall low efficiency rates. While progress in cannabis micropropagation has been made, nutrient imbalances and various disorders are still common. Successful micropropagation is species specific and dependent on a variety of interconnected factors related to abiotic conditions and nutrient availability, which represent challenges in the refinement and execution of effective methods. Micropropagation media represent the exclusive sources of macro- and micro-nutrients for cultured plant tissues, inadequacies of which can result in the emergence of morpho-physiological symptoms. This work represents the first in-depth analysis of multiple morpho-physiological disorders in micropropagated cannabis arising from media nutrient content. Additionally, we present machine learning as an effective tool for assessing nutrient-associated symptoms in cultured cannabis and identifying which components are responsible. Results will help with troubleshooting cannabis micropropagation systems to prevent or correct undesirable outcomes, while introducing new methods to assess in vitro cannabis disorders.

大麻最近作为一种重要的经济作物重新出现，推动了加强生产实践以满足市场需求的研究。发展中的大麻工业可以通过克服某些生产障碍，利用微繁殖在密闭空间内大量维持和繁殖无病原体植物，从而得到改善。然而，发展高效的大麻微繁殖系统一直受到各种形态生理障碍的阻碍，导致繁殖率低，培养下降，总体效率低。虽然大麻微繁殖取得了进展，但营养失衡和各种疾病仍然很常见。成功的微繁殖是物种特有的，依赖于各种与非生物条件和养分可用性相关的相互关联的因素，这代表了改进和执行有效方法的挑战。微繁培养基是培养植物组织宏量和微量营养素的唯一来源，缺乏这些营养素会导致形态生理症状的出现。这项工作首次深入分析了由培养基营养成分引起的微繁殖大麻的多种形态生理障碍。此外，我们提出机器学习作为评估培养大麻中营养相关症状和确定哪些成分负责的有效工具。结果将有助于排除大麻微繁殖系统，以防止或纠正不良后果，同时引入新的方法来评估体外大麻疾病。

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引用次数: 0

A metagenomic exploration of microbial distribution among the pond sediment, shrimp (Penaeus monodon) tissue and pond water: A special focus on nutrient cycling and shrimp viral pathogens 池塘沉积物、对虾（Penaeus monodon）组织和池塘水中微生物分布的宏基因组研究：特别关注营养循环和对虾病毒病原体

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-09-09 DOI: 10.1016/j.jgeb.2025.100568

Avik Sarkar , Rakesh Kr. Patra , Mathavan Muthaiyan , Ajoy Mallik

Penaeus monodon, a major shrimp species cultivated in Eastern Asia, has faced setbacks in aquaculture due to disease outbreaks. Metagenomic studies offer insights into the pond microbiome, revealing its critical roles in nutrient cycling, water quality, and disease control. This study investigated microbial communities in sediment, water, and shrimp samples from an aquaculture pond in Chaital, West Bengal, India. Shotgun metagenomic sequencing and bioinformatics analyses were used to assess taxonomic and functional profiles, focusing on nutrient cycling genes and viral populations. Sediments showed the highest microbial diversity, contributing to organic matter decomposition and ecological stability. Water microbes played roles in oxygenation and pathogen suppression, while shrimp tissues hosted microbes aiding digestion and immunity. Pathogenic viruses, especially Whispovirus (White Spot Syndrome Virus), dominated shrimp tissues. Functional gene analysis identified key pathways in carbon, nitrogen, and sulfur cycling. Integrating water quality data with microbiome profiles could help identify bioindicators and predict pathogen risks.

由于疾病爆发，东亚养殖的主要对虾品种单对虾在水产养殖方面面临挫折。宏基因组研究提供了对池塘微生物组的深入了解，揭示了其在营养循环、水质和疾病控制中的关键作用。本研究调查了印度西孟加拉邦Chaital一个水产养殖池塘沉积物、水和虾样品中的微生物群落。霰弹枪宏基因组测序和生物信息学分析用于评估分类和功能概况，重点关注营养循环基因和病毒种群。沉积物微生物多样性最高，有利于有机质分解和生态稳定。水微生物在氧化和抑制病原体中发挥作用，而虾组织中宿主的微生物有助于消化和免疫。致病性病毒，尤其是白斑综合征病毒（Whispovirus），在虾的组织中占主导地位。功能基因分析确定了碳、氮和硫循环的关键途径。将水质数据与微生物组资料相结合可以帮助识别生物指标并预测病原体风险。

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引用次数: 0

In-silico study on biomolecules derived from Cissus quadrangularis towards anti-inflammation 四棱草生物分子抗炎作用的硅片研究

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-09-09 DOI: 10.1016/j.jgeb.2025.100571

H. Nilofer Farjana , G. Mohan Valiathan , S. Mohanasatheesh

Objective

CD 163 protein is a macrophage-specific protein and hemoglobin scavenger receptor. Elevated expression of CD 163 protein in macrophages is an important feature indicating that tissues are subjected to inflammation. It can be an effective diagnostic biomarker for inflammatory diseases like gingivitis and periodontitis. Cissus quadrangularis is used as a potent alternative medicine to control inflammation and heal fractured bones. It has high osteogenic potential by increasing osteoblast mineralization. Being a natural product with a wide range of benefits can lead to drug development for its high anti-inflammatory and anti-osteoporotic properties. Studies have shown that Cissus quadrangularis has significant anti-inflammatory potential and can be used as a potential target for therapeutic agents. The objective of the study is to explore the interaction of the phytonutrients derived from Cissus quadrangularis with CD 163 protein to establish the anti-inflammatory activity by in silico approach.

Materials and methods

The biomolecules derived from the plant Cissus quadrangularis namely β-Sitosterol, luteolin, piceatannol, quadrangualrin, resveratrol, and tannins were subjected to molecular docking using Autodock of 4.0 PyRx software.

Results

The study results showed drug-likeness and binding affinity between biologically active Cissus quadrangularis molecules against inflammation associated with CD 163 protein.

Conclusion

It can be concluded that the biomolecules derived from Cissus quadrangularis exhibited anti-inflammatory potential and thus it can be further explored by invitro analysis in developing a new drug.

目的ecd 163蛋白是一种巨噬细胞特异性蛋白和血红蛋白清道夫受体。巨噬细胞中cd163蛋白的表达升高是表明组织遭受炎症的重要特征。它可以作为牙龈炎和牙周炎等炎症性疾病的有效诊断生物标志物。四角棘草是一种有效的替代药物，用于控制炎症和治愈骨折。通过增加成骨细胞矿化，具有较高的成骨潜能。作为一种具有广泛益处的天然产物，其高抗炎和抗骨质疏松的特性可以导致药物开发。研究表明，四角草具有显著的抗炎潜能，可作为治疗药物的潜在靶点。本研究的目的是通过硅片法研究四角蛇植物营养素与cd163蛋白的相互作用，以确定其抗炎活性。材料与方法利用4.0 PyRx软件Autodock对从四角草植物中提取的β-谷甾醇、木犀草素、皮杉醇、四角草素、白藜芦醇、单宁等生物分子进行分子对接。结果研究结果表明，具有生物活性的四角蛇分子具有抗cd163蛋白相关炎症的药物相似性和结合亲和力。结论四棱草生物分子具有抗炎活性，可通过体外分析进一步开发新药。

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引用次数: 0

Genetic differentiation and conservation insights into Salicornia iranica subsp. sinus-persica from Musa Bay using SCoT markers and DNA barcodes 中国水杨亚种的遗传分化与保护研究。使用SCoT标记和DNA条形码从Musa Bay提取了桃鼻草

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-09-03 DOI: 10.1016/j.jgeb.2025.100563

Fatemeh Nasernakhaei , Mahyar Zahraei

Understanding genetic diversity is essential for the conservation and management of halophyte species, especially in ecologically sensitive and industrially impacted regions. In this study, 52 accessions of Salicornia iranica subsp. sinus-persica were collected from diverse microhabitats across Musa Bay, including coastal zones, islands, and petrochemical sites. Following comprehensive morphological assessments showing high phenotypic similarity, and to ensure broad ecological coverage, 16 accessions representing all major microhabitats were selected for molecular analysis. SCoT markers and DNA barcoding (ITS and trnH-psbA regions) were employed to investigate their genetic structure. The SCoT markers revealed considerable polymorphism and partial genetic differentiation, especially between accessions from petrochemical and natural saline habitats. ITS sequencing identified five haplotypes without forming a monophyletic clade, indicating shallow divergence and possible ancestral polymorphism. In contrast, no variation was detected in the plastid region trnH-psbA. These results represent the first global report of ITS and trnH-psbA haplotypes for this subspecies. While the findings are preliminary due to limited sample size and marker coverage, they provide a critical baseline for future studies employing high-resolution genomic tools. Our findings underscore the influence of habitat fragmentation and anthropogenic pressures on genetic patterns and emphasize the importance of conserving genetically distinct accessions in ecologically vulnerable habitats to support the long-term survival and sustainable utilization of this coastal halophyte.

了解遗传多样性对盐生植物物种的保护和管理至关重要，特别是在生态敏感和受工业影响的地区。在本研究中，选取了52份海雀属植物。在Musa Bay的不同微生境中收集了sinus-persica，包括沿海地区、岛屿和石化场地。经过全面的形态学评估，发现表型相似性高，并确保广泛的生态覆盖，选择了16个代表所有主要微生境的材料进行分子分析。利用SCoT标记和DNA条形码（ITS和trnH-psbA区）对其遗传结构进行了研究。SCoT标记显示出相当大的多态性和部分遗传分化，特别是在石化和天然盐碱生境中。ITS测序鉴定出5个单倍型，但没有形成单系分支，表明浅分化和可能的祖先多态性。相比之下，在trnH-psbA质体区未检测到变异。这些结果是全球首次报道该亚种的ITS和trnH-psbA单倍型。虽然由于样本量和标记覆盖率有限，这些发现是初步的，但它们为使用高分辨率基因组工具的未来研究提供了关键的基线。我们的研究结果强调了生境破碎化和人为压力对遗传模式的影响，并强调了在生态脆弱的生境中保护遗传独特的物种对支持这种沿海盐生植物的长期生存和可持续利用的重要性。

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引用次数: 0

Phytochemical screening and inhibitory effects of Catha edulis Forsk extracts on oxidation, growth, biofilm and quorum sensing of selected pathogens Catha edulis Forsk提取物的筛选及其对病原菌氧化、生长、生物膜和群体感应的抑制作用

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-08-29 DOI: 10.1016/j.jgeb.2025.100560

Yonatan Nesru , Muktar Ahmed , Adam Mengistu , Mohammed Naimuddin

The treatment of infectious diseases is increasingly challenging due to the emergence and re-emergence of antibiotic-resistant bacteria. Plant-derived natural products are being explored as potential solutions to this global threat. This study investigated the phytochemical composition of Catha edulis and biological properties of its extracts, focusing on their potential as antioxidants and antimicrobials. Gas chromatography/mass spectrometry (GC/MS) of the methanol extract (MeOHE) fractions revealed 11 compounds in dichloromethane fraction (DCMF) and 30 compounds in ethyl acetate fraction (EAF), many of which possess bioactive functions as antioxidants and antimicrobials. Phytol was detected at 60.32 %, not reported before in C. edulis. The antioxidation potential of extracts was assayed by radical scavenging activity (RSA), and the IC₅₀ values ranged from 68.2 to 89.3 µg/mL, respectively indicating good antioxidant potency. In the disc diffusion assay against representative pathogenic bacteria, the extracts exhibited an inhibition order of EAF > DCMF > MeOHE in the 31–75 % range. The minimum inhibitory concentration (MIC) for MeOHE and DCMF were in the 0.5 − >1 mg/mL range, while the minimum bactericidal concentration (MBC) were from 2 to > 2 mg/mL. EAF showed a MIC and MBC in the 0.5–––1 mg/mL and 1–––2 mg/mL range, suggesting better inhibition potential. The tolerance test values showed bactericidal activity against all the tested bacteria. The minimum biofilm inhibitory concentration (MBIC) values of DCMF were 250 to > 500 µg/mL, while EAF ranged from 125 to 500 µg/mL, indicating effective inhibition of biofilm formation. The anti-quorum sensing (anti-QS) activity of DCMF and EAF on P. aeruginosa showed 55.8 % and 51.4 % inhibition of pyocyanin production at 500 µg/mL. These results suggest that DCMF and EAF of C. edulis are potent antioxidants and antimicrobials, potentially reducing the load of pathogens. To our best knowledge, this is the first report of antibiofilm and anti-QS activities of C. edulis extracts on pathogenic bacteria included in the Pathogen Priority List.

由于耐抗生素细菌的出现和重新出现，传染病的治疗越来越具有挑战性。人们正在探索植物衍生的天然产品，作为应对这一全球威胁的潜在解决方案。本文研究了Catha edulis的植物化学成分及其提取物的生物学特性，重点研究了其作为抗氧化剂和抗菌剂的潜力。甲醇提取物（MeOHE）组分的气相色谱/质谱（GC/MS）分析发现，二氯甲烷组分（DCMF）中有11个化合物，乙酸乙酯组分（EAF）中有30个化合物，其中许多化合物具有抗氧化剂和抗菌活性。叶绿醇的检出率为60.32%，未见报道。通过自由基清除活性（RSA）测定各提取物的抗氧化能力，其IC50值分别为68.2 ~ 89.3µg/mL，显示出较好的抗氧化能力。在对代表性致病菌的盘片扩散试验中，提取物对EAF >； DCMF >； MeOHE的抑制顺序为31 ~ 75%。对MeOHE和DCMF的最低抑菌浓度（MIC）为0.5 ~ 1mg /mL，最低杀菌浓度（MBC）为2 ~ 2mg /mL。EAF在0.5 ~ 1mg /mL和1 ~ 2mg /mL范围内显示MIC和MBC，表明有较好的抑制潜力。耐受性试验值显示出对所有被试细菌的杀菌活性。DCMF的最低生物膜抑制浓度（MBIC）为250 ~ 500µg/mL， EAF为125 ~ 500µg/mL，表明DCMF对生物膜的形成有有效的抑制作用。在500µg/mL浓度下，DCMF和EAF对P. aeruginosa的抗群体感应（anti-quorum sensing, qs）活性分别为55.8%和51.4%。这些结果表明，edulis的DCMF和EAF是有效的抗氧化剂和抗菌剂，可能减少病原体的负荷。据我们所知，这是第一次报道毛竹提取物对病原菌的抗菌膜和抗qs活性。

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引用次数: 0

Exploring the therapeutic potential of microRNAs: targeted gene regulation strategies for enhanced cancer therapy 探索microRNAs的治疗潜力：增强癌症治疗的靶向基因调控策略

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-08-28 DOI: 10.1016/j.jgeb.2025.100556

Shashwat Pandey , Preeti Yadav

Cancer remains one of the most significant causes of death worldwide, with conventional therapies often hindered by drug resistance, off-target effects, and systemic toxicity. MicroRNAs (miRNAs), small non-coding RNA molecules, have emerged as critical regulators of gene expression, influencing various cellular processes such as proliferation, apoptosis, and metastasis. Their dual role as tumor suppressors and oncogenes makes them promising therapeutic targets for cancer treatment. This review explores the therapeutic potential of miRNAs in modulating key cancer pathways and overcoming drug resistance. It delves into strategies like miRNA mimics and antagomiRs to restore normal gene expression, alongside advanced delivery systems, including nanoparticles and exosomes, to ensure targeted, stable, and effective delivery. Furthermore, the challenges associated with miRNA therapy, such as degradation, off-target effects, and immune responses, are discussed. The integration of miRNA-based approaches with existing therapies holds promise for enhancing treatment efficacy and paving the way for personalized cancer management.

癌症仍然是世界范围内最重要的死亡原因之一，传统的治疗方法往往受到耐药性、脱靶效应和全身毒性的阻碍。MicroRNAs （miRNAs）是一种小的非编码RNA分子，已成为基因表达的关键调节因子，影响各种细胞过程，如增殖、凋亡和转移。它们作为肿瘤抑制因子和致癌基因的双重作用使它们成为癌症治疗的有希望的治疗靶点。这篇综述探讨了mirna在调节关键癌症通路和克服耐药性方面的治疗潜力。它深入研究了miRNA模拟物和拮抗剂等策略，以恢复正常的基因表达，以及先进的递送系统，包括纳米颗粒和外泌体，以确保靶向，稳定和有效的递送。此外，还讨论了与miRNA治疗相关的挑战，如降解、脱靶效应和免疫反应。基于mirna的方法与现有疗法的整合有望提高治疗效果并为个性化癌症管理铺平道路。

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引用次数: 0

Exploration of Canarium odontophyllum fruit phytoconstituents as potential candidates against epilepsy using in silico studies 利用硅芯片研究探索牙齿Canarium odontophyum果实植物成分作为抗癫痫药物的潜在候选物

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-08-26 DOI: 10.1016/j.jgeb.2025.100561

Lim Joe Siang , Kamini Vijeepallam , Arunachalam Muthuraman , Parasuraman Pavadai , Thiruventhan Karunakaran , Veerasamy Ravichandran

Epilepsy is the third most common neurological illness worldwide, affecting over 65 million people. Current antiepileptic drugs (AEDs) are ineffective for about 30 % of epileptic patients. AED use is extremely expensive and associated with adverse effects such as ataxia, nystagmus, and central nervous system depression. Serious quality-of-life changes are another aspect of managing epileptic conditions. A variety of therapeutic plant components, including flavonoids, phenolics, terpenoids, and alkaloids, exhibit a wide range of biological actions, including antibacterial, antioxidant, antiulcer, antineoplastic, anti-inflammatory, antiepileptic, and immunostimulant properties. Because many of them are being researched in modern research to develop an active therapeutic candidate, we resorted to medicinal plants to investigate powerful antiepileptic components. So, in this in silico study, we aimed to identify potential antiepileptic agents from Canarium odontophyllum fruits using molecular docking, molecular dynamics, and ADMET prediction approach. In the present study, the virtual screening was performed on a set of 63 phytocompounds, identified from the hydroalcoholic (70 %) extract of C. odontophyllum fruit using LC-MS (LC-MS-QTOF) analysis, by using molecular docking simulation to examine their binding ability with seven proteins that are responsible for epilepsy. Further, the selected compounds with good binding affinity were subjected to ADMET screening to predict the pharmacokinetic properties, and molecular dynamics testing was done to confirm the outcomes. Our results revealed that C. odontophyllum phytoconstituents showed the strongest binding affinities against all tested enzymes. Moreover, kanzonol B could prominently interact with all tested proteins with moderate to good binding affinity (−6.70 to −10.65 kcal/mol). All top phytoconstituents obeyed Lipinski’s RO5 and drug-likeness properties. These computational studies suggested that the phytoconstituents of C. odontophyllum can serve as potent inhibitors of enzymes responsible for epilepsy. Thus, we recommend further experimental investigations to validate the promising results of the present in silico study.

癫痫是世界上第三大最常见的神经系统疾病，影响到6500多万人。目前的抗癫痫药物（AEDs）对大约30%的癫痫患者无效。AED的使用非常昂贵，并伴有不良反应，如共济失调、眼球震颤和中枢神经系统抑郁。严重的生活质量改变是控制癫痫病情的另一个方面。多种治疗性植物成分，包括黄酮类、酚类、萜类和生物碱，具有广泛的生物作用，包括抗菌、抗氧化、抗溃疡、抗肿瘤、抗炎、抗癫痫和免疫刺激等特性。由于现代研究正在研究其中的许多药物，以开发有效的治疗候选药物，因此我们利用药用植物来研究有效的抗癫痫成分。因此，在本计算机研究中，我们旨在通过分子对接、分子动力学和ADMET预测方法，从Canarium odontophyum果实中鉴定潜在的抗癫痫药物。在本研究中，采用LC-MS （LC-MS- qtof）分析方法，对从牙齿果（C. odontophyllum）果实的水酒精（70%）提取物中鉴定出的63种植物化合物进行虚拟筛选，并通过分子对接模拟来检测它们与7种导致癫痫的蛋白质的结合能力。进一步，选择结合亲和力较好的化合物进行ADMET筛选，预测药代动力学性质，并进行分子动力学测试来证实结果。结果表明，齿龈草植物成分对所有酶的结合亲和力最强。此外，kanzonol B与所有被测蛋白具有中等至良好的结合亲和力（- 6.70至- 10.65 kcal/mol）。所有顶级植物成分都符合利平斯基的RO5和药物相似特性。这些计算研究表明，牙齿草的植物成分可以作为癫痫酶的有效抑制剂。因此，我们建议进一步的实验研究来验证目前在硅研究中的有希望的结果。

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