Journal of Genetic Engineering and Biotechnology最新文献_第10页

Enhanced carotenoid accumulation in Chloroccocum humicola under controlled CO2 and light conditions 控制CO2和光照条件下腐殖质氯藻类胡萝卜素积累的增强

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-24 DOI: 10.1016/j.jgeb.2025.100619

Chatchai Kunyawut, Idtisak Paopo, Chakkrit Umpuch

Carotenoids are potent antioxidants and high-value bioactive compounds that green microalgae can efficiently synthesize. This study aimed to enhance carotenoid production in Chlorococcum humicola TISTR 8551 using a two-stage cultivation strategy in a 10-L air-lift photobioreactor (ALPBR), separating biomass accumulation and stress induction phases. During the “green stage” (9 days), cells were grown in modified BG-11 medium (N:P ratio 31:1) under 3,500 Lux white LED light with varying CO₂ concentrations (1–3 % v/v) to maximize biomass yield. In the subsequent “red stage” (15 days), environmental stressors, including elevated salinity and intensified light exposure, were applied to stimulate carotenoid biosynthesis. The highest total carotenoid content (38.72 ± 1.04 mg/L, 0.313 ± 0.018 mg/g biomass) was observed under 3 % CO₂ supplementation, likely due to enhanced photosynthetic carbon fixation and improved precursor availability via glucose metabolism. An optimal white light intensity of 25,000 Lux produced 32.03 ± 1.52 mg/L carotenoids. Additionally, the combination of 100,000 Lux white light with 1,600 Lux blue light significantly increased β-carotene content (6.98 ± 0.28 % of total carotenoids), while 2,400 Lux blue light yielded the highest astaxanthin level (5.01 ± 0.18 % of total carotenoids). These results highlight the synergistic effects of CO₂ enrichment, spectral light modulation, and stage-specific stress application in promoting targeted carotenoid biosynthesis. This study offers practical insights for optimizing large-scale microalgal pigment production in controlled photobioreactor systems.

类胡萝卜素是绿色微藻能够高效合成的抗氧化剂和高价值生物活性化合物。在10 l气升式光生物反应器（ALPBR）中，采用分离生物量积累阶段和胁迫诱导阶段的两阶段培养策略，提高humicola TISTR 8551的类胡萝卜素产量。在“绿色阶段”（9天），细胞在改性BG-11培养基（N:P比31:1）中生长，在3500 Lux白光LED灯下，以不同的CO2浓度（1 - 3% v/v）最大化生物质产量。在随后的“红色阶段”（15天），环境压力因素，包括盐度升高和光照增强，被用于刺激类胡萝卜素的生物合成。添加3% CO2时，类胡萝卜素总含量最高（38.72±1.04 mg/L, 0.313±0.018 mg/g生物量），这可能是由于光合作用固碳增强和葡萄糖代谢前体利用率提高所致。在25000 Lux的最佳白光强度下，类胡萝卜素的产量为32.03±1.52 mg/L。此外，100,000 Lux白光与1,600 Lux蓝光组合显著提高了β-胡萝卜素含量（占总类胡萝卜素的6.98±0.28%），而2,400 Lux蓝光产生的虾青素含量最高（占总类胡萝卜素的5.01±0.18%）。这些结果强调了CO2富集、光谱光调制和阶段特异性胁迫在促进靶向类胡萝卜素生物合成中的协同作用。该研究为在可控光生物反应器系统中优化大规模微藻色素生产提供了实践见解。

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引用次数: 0

Transcriptomics analysis unveils the complex interplay between diabetes and hypertension in regulating renal cell carcinoma pathway followed by pancreatic metastasis 转录组学分析揭示了糖尿病和高血压在调节肾细胞癌途径和胰腺转移过程中的复杂相互作用

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-29 DOI: 10.1016/j.jgeb.2025.100616

K.M. Tanjida Islam , Roksana Khanam , Aninda Roy , Ramisa Binti Mohiuddin , Jannati Akter , Samia Haque , Sheikh Abdullah Al Ashik , Saborni Sarker , A.K.M. Mohiuddin , Shahin Mahmud

Renal cell carcinoma (RCC) is often associated with metabolic disorders such as type 2 diabetes mellitus (T2DM) and hypertension. While existing research has established connections between these metabolic conditions and RCC, the underlying mechanisms driving RCC followed by pancreatic metastasis remain incompletely understood. Therefore, our study aimed to investigate the complex interplay between metabolic disorders (type 2 diabetes and hypertension) and malignancies (renal cell carcinoma and pancreatic cancer). To investigate the hidden link, we performed an integrative transcriptomic analysis. The analysis focuses only on T2DM and hypertension to identify a connection with the RCC pathway. Our analysis revealed that 190 significantly upregulated genes, of which MET emerged as a master regulator in RCC, while KRAS was the key regulator in pancreatic cancer. Furthermore, we identified key microRNAs (has-mir-1-3p, has-mir-16-5p, and has-mir-455-3p) and transcription factors (MBD1, TFDP1, and KLF9) regulate these targets. Additionally, we identified and validated CDC42, PTPN11, TGFB3, and MET as potential prognostic or theragnostic biomarkers. MET, KRAS, and PIK3CD emerged as the most promising therapeutic targets against a panel of 28 repurposable inhibitory drugs. The genetic and immune association suggested that CD8 + T cells are the key immune infiltrate significantly associated with poor survival outcomes in RCC and pancreatic cancer patients. Mutational analysis further highlighted the significance of KRAS G12C, G12V, and G12D mutations, which were common between RCC and pancreatic metastasis. Our study provides critical insights into the statistically significant associations between metabolic disorders and malignancies, emphasizing the potential of tailored therapies alongside shared therapies in managing RCC and its progression to pancreatic metastasis.

肾细胞癌（RCC）通常与代谢性疾病如2型糖尿病（T2DM）和高血压有关。虽然现有的研究已经建立了这些代谢条件与RCC之间的联系，但驱动RCC随后胰腺转移的潜在机制仍然不完全清楚。因此，我们的研究旨在探讨代谢紊乱（2型糖尿病和高血压）与恶性肿瘤（肾细胞癌和胰腺癌）之间复杂的相互作用。为了研究隐藏的联系，我们进行了综合转录组分析。该分析仅关注T2DM和高血压，以确定与RCC途径的联系。我们的分析显示，190个基因显著上调，其中MET是RCC的主要调控因子，而KRAS是胰腺癌的关键调控因子。此外，我们确定了关键的microrna （has-mir-1-3p， has-mir-16-5p和has-mir-455-3p）和转录因子（MBD1， TFDP1和KLF9）调节这些靶点。此外，我们鉴定并验证了CDC42、PTPN11、TGFB3和MET作为潜在的预后或诊断生物标志物。MET、KRAS和PIK3CD是对28种可重复使用的抑制药物最有希望的治疗靶点。遗传和免疫关联表明，CD8 + T细胞是与RCC和胰腺癌患者生存预后不良显著相关的关键免疫浸润。突变分析进一步强调了KRAS G12C、G12V和G12D突变的意义，这些突变在RCC和胰腺转移之间是常见的。我们的研究为代谢性疾病和恶性肿瘤之间的统计学意义相关性提供了重要见解，强调了定制治疗和共享治疗在控制RCC及其进展到胰腺转移方面的潜力。

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引用次数: 0

Bioinformatics-driven identification of pathogenic missense nsSNPs in the human proto-oncogene SRC and cancer susceptibility 生物信息学驱动的人类原癌基因SRC致病性错义nssnp鉴定和癌症易感性

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-11-29 DOI: 10.1016/j.jgeb.2025.100618

Md. Shakil Ahamed, Roksana Khanam, K.M. Tanjida Islam, Fahmida Tabassum, Md. Al Amin, Jannatul Fardous, Nadira Hoque Tashpie, A.K.M. Mohiuddin, Shahin Mahmud

SRC is a proto-oncogene that regulates cell proliferation and survival, and its dysregulation is commonly observed in diverse cancers. While SRC kinase dysregulation is well-established as a cancer driver, the functional consequences of its genetic variants, particularly non-synonymous single-nucleotide polymorphisms (nsSNPs) are not fully understood. Therefore, we employed an integrative computational approach to identify nsSNPs in SRC and analyze their impact on protein function and structure. Out of the 512 missense nsSNPs analyzed, 42 were predicted to be deleterious, with 12 likely to destabilize protein structure. Among these, three mutations, namely W151C (rs746439256), Y419N (rs2147125119), and P465S (rs1251532695), were particularly significant, causing substantial physicochemical changes. Molecular dynamics simulations revealed that these variations reduce protein stability and flexibility, resulting in conformational alterations. Docking study demonstrated that these mutations disrupt the binding interface residues of the SRC-FAK complex and affect dasatinib binding affinity. Additionally, gene expression analysis linked mutated SRC to dysregulation of cancer-related genes, especially in multiple myeloma and uterine cancer, and suggested reciprocal regulation by other mutated genes across malignancies. These findings highlight the oncogenic potential of SRC mutations and pave the way for future population-based studies exploring their role as diagnostic biomarkers, therapeutic targets, and modulators of drug response in personalized cancer treatment.

SRC是一种调节细胞增殖和存活的原癌基因，其失调在多种癌症中都很常见。虽然SRC激酶失调被认为是癌症驱动因素，但其遗传变异，特别是非同义单核苷酸多态性（nssnp）的功能后果尚未完全了解。因此，我们采用综合计算方法鉴定SRC中的nssnp，并分析其对蛋白质功能和结构的影响。在分析的512个错义nssnp中，预计有42个是有害的，其中12个可能破坏蛋白质结构的稳定。其中W151C （rs746439256）、Y419N （rs2147125119）和P465S （rs1251532695）三个突变尤为显著，引起了较大的理化变化。分子动力学模拟显示，这些变化降低了蛋白质的稳定性和灵活性，导致构象改变。对接研究表明，这些突变破坏了SRC-FAK复合物的结合界面残基，影响了达沙替尼的结合亲和力。此外，基因表达分析将SRC突变与癌症相关基因的失调联系起来，特别是在多发性骨髓瘤和子宫癌中，并表明其他突变基因在恶性肿瘤中相互调节。这些发现强调了SRC突变的致癌潜力，并为未来基于人群的研究铺平了道路，探索它们在个性化癌症治疗中作为诊断生物标志物、治疗靶点和药物反应调节剂的作用。

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引用次数: 0

Isolation and characterization of peroxidase from potato leaves Solanum tuberosum: Application in glucose diagnostic kit 马铃薯叶片中过氧化物酶的分离与鉴定：在葡萄糖诊断试剂盒中的应用

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-09-30 DOI: 10.1016/j.jgeb.2025.100584

Hassan M.M. Masoud , Mohammed M. Abdel-Monsef , Mohamed S. Helmy , Sayed S. Esa , Doaa A. Darwish

Peroxidases play a pivotal role in many medical applications such as diagnostic kits and ELISA assays. This study reports the purification and biochemical characterization of peroxidase from potato leaves (PLPOD) and its application in the formulation of a glucose diagnostic kit. PLPOD was purified through CM-cellulose ion-exchange and Sephacryl S-300 gel filtration chromatography, achieving an 11.8-fold purification with 48 % recovery and a final specific activity of 705.7 U/mg. Native PAGE and activity staining confirmed the enzyme’s purity and homogeneity. PLPOD molecular weight was estimated from gel filtration column as 64 kDa, but on SDS gel, there were three PLPOD isoforms of approximated molecular weights ranging from ∼ 40–60 kDa. PLPOD exhibited optimal activity at pH 5.2, with Zn²⁺ and Ni²⁺ enhancing activity, while Ca²⁺ and Fe²⁺ inhibited it. Inhibitor analysis confirmed the heme-dependent nature of the enzyme. The K_m values for guaiacol and H₂O₂ were 0.067 mM and 40 mM, respectively, consistent with typical plant peroxidases. A glucose diagnostic kit developed using PLPOD showed strong concordance with a commercial glucose kit when tested on normal and diabetic serum samples demonstrating its clinical applicability. These findings suggest that PLPOD is a viable cost-effective for use in diagnostic assays and kits.

过氧化物酶在许多医学应用中发挥着关键作用，如诊断试剂盒和酶联免疫吸附试验。本研究报道了马铃薯叶片过氧化物酶（PLPOD）的纯化、生化特性及其在葡萄糖诊断试剂盒中的应用。通过cm -纤维素离子交换和sepphacryl S-300凝胶过滤层析纯化PLPOD，纯化率为11.8倍，回收率为48%，最终比活性为705.7 U/mg。天然PAGE和活性染色证实了酶的纯度和均匀性。从凝胶过滤柱上估计PLPOD的分子量为64 kDa，但在SDS凝胶上，PLPOD的三种异构体的近似分子量在~ 40-60 kDa之间。PLPOD在pH 5.2时活性最佳，Zn2+和Ni2+增强了PLPOD活性，Ca2+和Fe2+抑制了PLPOD活性。抑制剂分析证实了该酶的血红素依赖性。愈创木酚和H2O2的Km值分别为0.067 mM和40 mM，与典型植物过氧化物酶一致。使用PLPOD开发的葡萄糖诊断试剂盒在对正常和糖尿病血清样本进行测试时显示出与商业葡萄糖试剂盒的强一致性，证明了其临床适用性。这些发现表明，PLPOD在诊断分析和试剂盒中是一种具有成本效益的可行方法。

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引用次数: 0

Genome-wide identification and evolutionary analysis of SUT genes reveals key regulators of drought stress response in finger millet (Eleusine coracana) 谷子SUT基因全基因组鉴定及进化分析揭示干旱胁迫响应的关键调控因子

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-10-14 DOI: 10.1016/j.jgeb.2025.100592

Kasinathan Rakkammal , Pandiyan Muthuramalingam , Hyunsuk Shin , Manikandan Ramesh

Sucrose transporters (SUTs) mediate sucrose movement across plant membranes, playing a crucial role in carbon allocation and stress responses. Although finger millet (Eleusine coracana) is known for its inherent drought resistance, the specific involvement of SUT genes in this characteristic is still unclear. This study aimed to identify the SUT genes of millet and to assess their expression in drought conditions. Five SUT genes (EcSUT1-EcSUT5) were identified that encode proteins with 9–12 transmembrane domains. Phylogenetic analysis clustered these SUT members across all three main SUT groups, suggesting an evolutionary divergence within the family. Synteny analysis revealed conserved genomic regions, with EcSUT2 showing 91–94% identity with orthologs in closely related grasses. Structural models further confirmed their typical transmembrane architecture. Interaction analysis identified EcSUT2 as a key interaction with SWEET transporters. Furthermore, the promoter regions of EcSUT2 and EcSUT5 were found to be enriched with hormone and stress-responsive elements. Under drought conditions, EcSUT1-EcSUT4 displayed transient induction, while EcSUT5 showed sustained upregulation, especially in the roots, notably after 48 h. The finger millet SUT family exhibits evolutionary conservation within grasses, with individual genes that play different roles in stress response. The persistent upregulation of EcSUT5 under drought strongly suggests its involvement in maintaining sucrose transport during long-term adverse conditions. This candidate gene requires further functional validation to uncover the stress dynamics for sustainable crop improvement.

蔗糖转运体（SUTs）介导蔗糖在植物膜上的运动，在碳分配和逆境响应中起着至关重要的作用。虽然小米（Eleusine coracana）以其固有的抗旱性而闻名，但SUT基因在这一特性中的具体参与尚不清楚。本研究旨在鉴定谷子SUT基因并评估其在干旱条件下的表达。5个SUT基因（EcSUT1-EcSUT5）编码9-12个跨膜结构域的蛋白。系统发育分析将这些SUT成员聚集在所有三个主要SUT组中，表明家族内部存在进化分歧。Synteny分析显示保守的基因组区域，EcSUT2与近缘草本同源物的同源性为91-94%。结构模型进一步证实了它们典型的跨膜结构。相互作用分析确定EcSUT2是与SWEET转运蛋白的关键相互作用。此外，EcSUT2和EcSUT5的启动子区域被发现富含激素和应激反应元件。干旱条件下，EcSUT1-EcSUT4表现为瞬时诱导，而EcSUT5表现为持续的上调，尤其是在根系中，尤其是在48 h后。谷子SUT家族在禾本科植物中表现出进化保守性，个体基因在胁迫响应中发挥不同的作用。干旱条件下EcSUT5的持续上调强烈提示其参与维持长期不利条件下的蔗糖运输。该候选基因需要进一步的功能验证，以揭示可持续作物改良的胁迫动态。

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引用次数: 0

Elucidating the functional significance of catalytic and chitin-binding domains for the anti-cancer property of a bacterial endochitinase 阐明催化和几丁质结合结构域对细菌几丁质内切酶抗癌特性的功能意义

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-10-13 DOI: 10.1016/j.jgeb.2025.100596

Ankita Shrivastava , Manik Goel , Md Fahim Khalid, Priyamedha Yadav, Suroj Maharjan, Rinkoo Devi Gupta

Chitinases are enzymes that facilitate the breakdown of chitin and also interact with carbohydrate moieties such as heparin sulphate due to structural similarity with chitin, thereby influencing cell adherence and migration. Bacterial endo-chitinase contains several domains, such as a catalytic domain, an FNIII domain, and a chitin-binding domain. Recent studies have demonstrated the anti-cancer property of catalytically active chitinases; however, the mechanism and targets have not yet been explored. Therefore, this study investigates the importance of the catalytic and chitin-binding domains of a bacterial endo-chitinase (ChiC) for the anti-cancer property by creating domain-specific mutants. Initially, an in silico study identified an evolutionarily conserved tryptophan (W300) within the catalytic cleft of ChiC, which was subjected to site-directed mutagenesis to create an inactive ChiC mutant. Similarly, another ChiC mutant was created without the chitin-binding domain. The recombinantly expressed WT and mutant ChiC proteins were utilized to analyze their effects on the cell viability, cell cycle, and migratory and invasive behaviour of MCF-7 cells. The mutants of ChiC, lacking either catalytic property or chitin-binding domain, resulted in a loss of their ability to inhibit cell proliferation and migration. These observations suggest that the catalytic and chitin-binding domains are essential for exhibiting anti-proliferative and anti-migratory effects, providing insights for future therapeutic applications.

几丁质酶是一种促进几丁质分解的酶，由于与几丁质结构相似，几丁质酶还能与硫酸肝素等碳水化合物相互作用，从而影响细胞粘附和迁移。细菌内切几丁质酶包含几个结构域，如催化结构域、FNIII结构域和几丁质结合结构域。近年来的研究表明，催化活性几丁质酶具有抗癌特性；然而，其作用机制和作用靶点尚未探索。因此，本研究通过创建结构域特异性突变体来研究细菌内切几丁质酶（ChiC）的催化和几丁质结合结构域对抗癌特性的重要性。最初，一项计算机研究在ChiC的催化裂口中发现了一个进化上保守的色氨酸（W300），该色氨酸受到定点诱变以产生无活性的ChiC突变体。同样，另一个没有几丁质结合结构域的ChiC突变体被创造出来。利用重组表达的WT和突变的ChiC蛋白分析其对MCF-7细胞活力、细胞周期、迁移和侵袭行为的影响。ChiC突变体缺乏催化特性或几丁质结合结构域，导致其抑制细胞增殖和迁移的能力丧失。这些观察结果表明，催化和几丁质结合结构域对于显示抗增殖和抗迁移作用是必不可少的，为未来的治疗应用提供了见解。

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引用次数: 0

Enzymatic characterization and proteomic profiling of venoms from the medically important Androctonus species 从医学上重要的雄蛾物种毒液的酶特性和蛋白质组学分析

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-09-10 DOI: 10.1016/j.jgeb.2025.100566

Alhussin M.A. Megaly , Masahiro Miyashita , Abdulaziz R. Alqahtani , Mohammed Abdel-Wahab

Egyptian scorpions of the Androctonus genus (family Buthidae) produce life-threatening stings owing to their neurotoxic venom. However, the composition and enzymatic activities of their venoms remain poorly understood: We used electrophoresis to analyze the protein components of venoms collected from three Androctonus species: Androctonus amoreuxi, Androctonus australis, and Androctonus bicolor. Mass spectrometric analysis was performed to characterize the peptides present in these venoms. The phospholipase A₂ (PLA₂), hyaluronidase, and protease activities of the venoms were examined to gauge their potential contribution to venom toxicity. Finally, the antibacterial and hemolytic activities of the venoms were evaluated. The electrophoretic profiles of the three venoms showed features specific to each species, with distinct protein bands observed at 75, 74, 67, 48, 46, 40, and 28 kDa, along with a notable band above the 15-kDa mark. Liquid chromatography/mass spectrometry analyses were used to detect the presence of 369, 324, and 351 components in with molecular masses in the range of 500–10,000 Da in the venoms of A. amoreuxi, A. australis, and A. bicolor, respectively. Disulfide-rich peptides (three disulfide bridges) were abundant, but peptides without disulfide bonds were also detected in all venom samples. All three venoms exhibited hyaluronidase activities, whereas protease and PLA₂ activities were either weak (at 1 µg and 10 µg) or undetectable, even at higher concentrations (up to 20 µg). All assays were performed using venoms standardized by dry weight to ensure consistent protein quantities. Crude venoms of A. amoreuxi and A. australis showed antibacterial activity against E. coli and B. subtilis (5–10 μg), whereas A. bicolor required 10 μg. Hydrophobic fractions (40–55 min) of A. australis alone retained this activity. This work furthers our knowledge of the enzymatic and peptide composition of Androctonus venoms, unveiling their potential in drug delivery enhancement and other biomedical applications. These findings will inform the development of better strategies for the treatment and prevention of scorpion envenomation.

埃及蝎属（蝎科）由于其神经毒性毒液产生危及生命的刺。然而，其毒液的组成和酶活性仍然知之甚少：我们使用电泳分析了三种雄蛾的毒液的蛋白质成分：amoreuxi雄蛾，australis雄蛾和双色雄蛾。质谱分析进行表征肽存在于这些毒液。检测了毒液的磷脂酶A2 （PLA2）、透明质酸酶和蛋白酶活性，以评估它们对毒液毒性的潜在贡献。最后，对毒液的抗菌和溶血活性进行了评价。三种毒液的电泳图谱显示出每个物种特有的特征，在75、74、67、48、46、40和28 kDa处观察到不同的蛋白质条带，并在15 kDa标记以上观察到显著的条带。采用液相色谱/质谱分析方法，分别检测到amoreuxi、A. australis和A. bicolor毒液中存在369、324和351种分子质量在500 ~ 10000 Da范围内的成分。在所有毒液样品中均检测到丰富的二硫肽（3个二硫桥），但也检测到不含二硫键的肽。所有三种毒液都显示出透明质酸酶活性，而蛋白酶和PLA2活性要么很弱（在1µg和10µg时），要么无法检测到，即使在较高浓度（高达20µg）下也是如此。所有检测均使用按干重标准化的毒液进行，以确保蛋白质数量一致。粗毒液对大肠杆菌和枯草芽孢杆菌的抑菌活性为5 ~ 10 μg，对双色拟南芥的抑菌活性为10 μg。疏水部分（40-55 min）单独的南胡麻保持这种活性。这项工作进一步加深了我们对雄蛾毒液的酶和肽组成的了解，揭示了它们在药物输送增强和其他生物医学应用方面的潜力。这些发现将为蝎子中毒的治疗和预防提供更好的策略。

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引用次数: 0

Comprehensive examination of demographic, psychological, cognitive, biochemical, and genetic profiles of methamphetamine addicts 甲基苯丙胺成瘾者的人口学、心理、认知、生化和遗传特征的综合检查

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-10-07 DOI: 10.1016/j.jgeb.2025.100564

Haider K. Hussain , Yolanda Loarce Tejada , Anna Barbaro

Methamphetamine (MA) addiction is a serious public health concern with wide-ranging neurobiological and behavioral effects. This study aimed to assess the demographic, psychological, cognitive, biochemical, and genetic profiles of individuals with methamphetamine dependence, focusing on neurotransmitter levels and the expression of addiction- and aggression-related genes. Sixty male methamphetamine users and thirty age-matched healthy controls were recruited. Participants underwent psychological assessments, cognitive testing, and biochemical evaluation of serotonin and dopamine levels using ELISA. Gene expression of SLC6A4 and COMT was quantified via real-time PCR. Significant alterations were observed in the methamphetamine group compared to controls, including reduced serotonin (17.1 ± 3.1 vs. 20.5 ± 3.2 ng/mL; p = 0.002) and dopamine levels (46.3 ± 7.2 vs. 52.4 ± 6.5 ng/mL; p = 0.015), as well as down-regulation of SLC6A4 (0.64-fold vs. 1.00; p = 0.001) and up-regulation of COMT (1.47-fold vs. 1.00; p = 0.028). These biochemical and genetic changes were correlated with increased aggression and cognitive impairments. The findings underscore the impact of prolonged MA use on neurochemical balance and gene expression, contributing to the development of aggressive behaviors and addictive patterns. Tailored treatment strategies that integrate genetic and psychological profiling, along with longitudinal monitoring, are essential to address the multifactorial nature of methamphetamine addiction and improve clinical outcomes.

甲基苯丙胺（MA）成瘾是一个严重的公共卫生问题，具有广泛的神经生物学和行为影响。本研究旨在评估甲基苯丙胺依赖个体的人口学、心理、认知、生化和遗传特征，重点关注神经递质水平和成瘾和攻击相关基因的表达。招募了60名男性冰毒使用者和30名年龄匹配的健康对照者。参与者使用ELISA进行了心理评估、认知测试和血清素和多巴胺水平的生化评估。实时荧光定量PCR检测SLC6A4和COMT的基因表达。与对照组相比，甲基苯丙胺组血清素（17.1±3.1比20.5±3.2 ng/mL, p = 0.002）和多巴胺水平（46.3±7.2比52.4±6.5 ng/mL, p = 0.015）降低，SLC6A4下调（0.64倍比1.00,p = 0.001）， COMT上调（1.47倍比1.00,p = 0.028）。这些生化和遗传变化与攻击性和认知障碍的增加有关。研究结果强调了长期使用MA对神经化学平衡和基因表达的影响，有助于攻击性行为和成瘾模式的发展。结合遗传和心理分析以及纵向监测的量身定制的治疗策略对于解决甲基苯丙胺成瘾的多因素性质和改善临床结果至关重要。

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引用次数: 0

Phenotyping analysis of rice cv. Nipponbare overexpressing OsMADS56 gene in drought tolerance, plant height, and yield components 水稻变异基因表型分析。OsMADS56基因在耐旱性、株高和产量成分中的过表达

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-09-11 DOI: 10.1016/j.jgeb.2025.100570

Dini Nurdiani, Dwi Widyajayantie, Enung Sri Mulyaningsih, Amy Estiati, Satya Nugroho

Background

The MADS-box protein family is a transcription factor (TF) family whose members harbour a MADS-box domain located in their N-terminal. This domain consists of 60 amino acids and functions in DNA binding. In plants, these family genes broadly regulate plant growth, development and responses to various environmental stresses. In this study, an OsMADS56 (702 bp) was isolated from rice (Oryza sativa cv. Nipponbare) to investigate its role in response to drought stress and other agronomic phenotypes at both the vegetative and generative stages.

Results

Rice mutants harbouring overexpressed OsMADS56 gene driven by the constitutive 35S promoter were generated. Quantitative real-time (qRT)-PCR confirmed the overexpression of OsMADS56 at T1 (M76.7) generation. Observation of the T2 OsMADS56-overexpressed rice line (OX7) showed improved tolerance to drought stress. It also showed different agronomic phenotypes compared to wild type (WT), such as shorter plant height (PH) and improved productivity as indicated by earlier heading date (HD), a higher number of panicles (NP), a higher number of grains (NG) and higher grain yield (GY) than WT.

Conclusion

The findings suggest that OsMADS56 was implicated in the rice response to PEG-induced drought stress, rice plant height, and yield components. The OsMADS56 gene and the characterized line can be utilized in further studies on rice adaptation against abiotic stress and its productivity.

MADS-box蛋白家族是一个转录因子（TF）家族，其成员在其n端含有MADS-box结构域。该结构域由60个氨基酸组成，在DNA结合中起作用。在植物中，这些家族基因广泛调控植物的生长、发育和对各种环境胁迫的反应。本研究从水稻（Oryza sativa cv.）中分离到OsMADS56基因（702 bp）。Nipponbare)研究其在营养和生殖阶段对干旱胁迫和其他农艺表型的响应中的作用。结果在组成型35S启动子驱动下获得了OsMADS56基因过表达的水稻突变体。qRT -PCR证实OsMADS56在T1 （M76.7）代过表达。T2 osmads56过表达水稻品系（OX7）对干旱胁迫的耐受性提高。与野生型（WT）相比，OsMADS56表现出不同的农学表型，如较短的株高（PH）、较早的抽穗期（HD）、较高的穗数（NP）、较高的粒数（NG）和较高的产量（GY）。结论OsMADS56参与了水稻对peg诱导的干旱胁迫、水稻株高和产量的响应。OsMADS56基因及其鉴定品系可用于水稻对非生物胁迫的适应性及其产量的进一步研究。

{"title":"Phenotyping analysis of rice cv. Nipponbare overexpressing OsMADS56 gene in drought tolerance, plant height, and yield components","authors":"Dini Nurdiani, Dwi Widyajayantie, Enung Sri Mulyaningsih, Amy Estiati, Satya Nugroho","doi":"10.1016/j.jgeb.2025.100570","DOIUrl":"10.1016/j.jgeb.2025.100570","url":null,"abstract":"<div><h3>Background</h3><div>The MADS-box protein family is a transcription factor (TF) family whose members harbour a MADS-box domain located in their N-terminal. This domain consists of 60 amino acids and functions in DNA binding. In plants, these family genes broadly regulate plant growth, development and responses to various environmental stresses. In this study, an <em>OsMADS56</em> (702 bp) was isolated from rice (<em>Oryza sativa</em> cv. Nipponbare) to investigate its role in response to drought stress and other agronomic phenotypes at both the vegetative and generative stages.</div></div><div><h3>Results</h3><div>Rice mutants harbouring overexpressed <em>OsMADS56</em> gene driven by the constitutive 35S promoter were generated. Quantitative real-time (qRT)-PCR confirmed the overexpression of <em>OsMADS56</em> at T1 (M76.7) generation. Observation of the T2 <em>OsMADS56</em>-overexpressed rice line (OX7) showed improved tolerance to drought stress. It also showed different agronomic phenotypes compared to wild type (WT), such as shorter plant height (PH) and improved productivity as indicated by earlier heading date (HD), a higher number of panicles (NP), a higher number of grains (NG) and higher grain yield (GY) than WT.</div></div><div><h3>Conclusion</h3><div>The findings suggest that <em>OsMADS56</em> was implicated in the rice response to PEG-induced drought stress, rice plant height, and yield components. The <em>OsMADS56</em> gene and the characterized line can be utilized in further studies on rice adaptation against abiotic stress and its productivity.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 4","pages":"Article 100570"},"PeriodicalIF":2.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular insights into charantin and β-carotene biosynthesis in bitter gourd (Momordica charantia L.): Gene expression dynamics and metabolite profiling 苦瓜（Momordica charantia L.）中charantin和β-胡萝卜素生物合成的分子分析：基因表达动态和代谢谱

IF 2.8 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology

Pub Date : 2025-12-01 Epub Date: 2025-12-02 DOI: 10.1016/j.jgeb.2025.100626

Banoth Tharun , Gograj Singh Jat , Manisha Mangal , Vishal Sunartiya , Sachin Kumar , Rakesh Bhardwaj , Naveen Singh , Jeetendra Kumar Ranjan , Avinash Tomer , Deepak Singh , Tusar Kanti Behera

Bitter gourd is recognized for its anticancer and antidiabetic properties, largely attributed to charantin, and is also a rich source of carotenoids, including β-carotene. In this study, considerable variation was observed in both the accumulation and gene expression patterns associated with charantin and β-carotene biosynthesis across diverse genotypes. PVGy-201 exhibited the strongest gene expression profile, with the highest transcript levels for five key genes, including McIDI (∼16,493-fold), McPMK (∼694-fold), and McSE (∼466-fold), which corresponded with the maximum charantin content (38.53 µg/g FW). Similarly, DBGS-2 showed elevated expression of McHMGR1 (∼271-fold) and McMK (∼13-fold), supporting its high charantin accumulation (35.27 µg/g FW). The wild species Momordica balsamina demonstrated strong expression of McHMGR2 and McSE, consistent with charantin content of 29.36 µg/g FW. For β-carotene, DBGS-21-06 recorded peak expression of McPSY (∼17.2-fold), McZDS (∼4.9-fold), and McCHXB (∼2.8-fold), aligning with high carotenoid levels at both edible (18.46 µg/g FW) and ripening (52.31 µg/g FW) stages. Pusa Rasdar showed elevated expression of McZEP (∼6.9-fold) and McPDS (∼10.8-fold), correlating with maximum carotenoid content (19.49 µg/g FW at edible stage and 55.66 µg/g FW at ripening). DBGS-100-0 expressed McLCYE1 (∼2.9-fold) and McLCYE2 (∼3.1-fold), with high carotenoids (15.91 µg/g FW) at the edible stage. Collectively, PVGy-201 and DBGS-2 were identified as promising candidates for charantin enrichment, while DBGS-21-06 and Pusa Rasdar emerged as superior for β-carotene accumulation. These genotype-specific insights provide a molecular framework to support marker-assisted selection, transcriptome-based screening, and metabolic engineering for the development of nutritionally enhanced bitter gourd cultivars with stable metabolite profiles.

苦瓜以其抗癌和抗糖尿病的特性而闻名，这主要归功于charantin，它也是类胡萝卜素的丰富来源，包括β-胡萝卜素。在这项研究中，在不同的基因型中，与charantin和β-胡萝卜素生物合成相关的基因积累和表达模式都有很大的差异。PVGy-201表现出最强的基因表达谱，五个关键基因的转录水平最高，包括McIDI（~ 16,493倍），McPMK（~ 694倍）和McSE（~ 466倍），这与最大charantin含量（38.53µg/g FW）相对应。同样，DBGS-2显示McHMGR1（~ 271倍）和McMK（~ 13倍）的表达升高，支持其高charantin积累（35.27µg/g FW）。野生苦瓜（Momordica balsamina） mcchmgr2和McSE表达较强，与charantin含量29.36µg/g FW一致。对于β-胡萝卜素，DBGS-21-06记录了McPSY（~ 17.2倍）、McZDS（~ 4.9倍）和McCHXB（~ 2.8倍）的峰值表达，在可食用（18.46µg/g FW）和成熟（52.31µg/g FW）阶段均具有高水平的类胡萝卜素。Pusa Rasdar显示McZEP（~ 6.9倍）和McPDS（~ 10.8倍）的表达升高，与最大类胡萝卜素含量（食用期19.49µg/g FW，成熟期55.66µg/g FW）相关。DBGS-100-0表达McLCYE1（~ 2.9倍）和McLCYE2（~ 3.1倍），在食用阶段具有高的类胡萝卜素（15.91µg/g FW）。总的来说，PVGy-201和DBGS-2被认为是charantin富集的有希望的候选物质，而DBGS-21-06和Pusa Rasdar被认为是β-胡萝卜素积累的优势物质。这些基因型特异性的见解为支持标记辅助选择、基于转录组的筛选和代谢工程提供了分子框架，以开发具有稳定代谢物谱的营养增强苦瓜品种。

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引用次数: 0