Pub Date : 2021-01-01DOI: 10.1615/plasmamed.2021040820
H. Matsuura, Nguyen Tran Trung, Bounyang Ouanthavinsak, J. Sakamoto, Y. Takemura, R. Asada, M. Furuta
: Polyvinyl alcohol–potassium iodine (PVA-KI) solution as a radiation dose meter has been proposed because it shows clear color changes in the presence of reactive radicals. Here, PVA-KI is studied to visualize the production and transport of radicals during the interaction between the atmospheric pressure plasma jet and the solution. First, we focus on the challenges of storing the PVA-KI sample solution at different temperatures. The reproducibility of the PVA-KI color reaction depends on this condition. Additionally, preliminary findings on the radical produc tion mechanism and radical types are provided.
{"title":"Reactive radical study using the polyvinyl alcohol–potassium iodide solution as a new chemical probe","authors":"H. Matsuura, Nguyen Tran Trung, Bounyang Ouanthavinsak, J. Sakamoto, Y. Takemura, R. Asada, M. Furuta","doi":"10.1615/plasmamed.2021040820","DOIUrl":"https://doi.org/10.1615/plasmamed.2021040820","url":null,"abstract":": Polyvinyl alcohol–potassium iodine (PVA-KI) solution as a radiation dose meter has been proposed because it shows clear color changes in the presence of reactive radicals. Here, PVA-KI is studied to visualize the production and transport of radicals during the interaction between the atmospheric pressure plasma jet and the solution. First, we focus on the challenges of storing the PVA-KI sample solution at different temperatures. The reproducibility of the PVA-KI color reaction depends on this condition. Additionally, preliminary findings on the radical produc tion mechanism and radical types are provided.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67349049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1615/plasmamed.2021041420
Jamoliddin Razzokov, Sunnatullo Fazliev, M. Yusupov, A. Sharipov, Zukhriddin Ruziev, Sh. I. Mamatkulov
: Cytoglobin (Cygb) is a recently discovered member of the globin family. Cygb maintains a large apolar cavity with the heme group located in it. The main catalytic activity of Cygb takes place around this heme group, which is also considered as a ligand docking station. Biochemical experiments revealed that Cygb can scavenge reactive oxygen and nitrogen species, such as hydrogen peroxide, nitric oxide, and peroxinitrite, protecting the cell against oxidative and nitrosative stress. However, the effect of mutation, as well as its synergistic effect together with oxidation on scavenging activity of the Cygb, has not yet been studied in detail. Thus, in this research we perform molecular dynamics and docking simulations to study the impact of mutation and oxidation on the Cygb catalytic function. Our simulation results show that the mutation of lysine 80 residue to alanine in Cygb results in an opening of the access to the heme group, thereby increasing its scavenging function. Moreover, the combination of this mutation (i.e., Cygb (L80A) ) with the oxidation [namely, the disulfide bond formation in Cygb (i.e., Cygb S-S )] induces the complex conformational changes in its structure. As a result, these changes lead to even more opening of the access to the heme group, which in turn enhances the scavenging activity of Cygb to a more extent. The latter can be a hallmark of enhanced enzymatic function of the modified Cygb (i.e., Cygb (L80A) and Cygb S-S ).
{"title":"Effect of mutation and disulfide bond formation on the catalytic site of monomeric cytoglobin: a molecular level insight","authors":"Jamoliddin Razzokov, Sunnatullo Fazliev, M. Yusupov, A. Sharipov, Zukhriddin Ruziev, Sh. I. Mamatkulov","doi":"10.1615/plasmamed.2021041420","DOIUrl":"https://doi.org/10.1615/plasmamed.2021041420","url":null,"abstract":": Cytoglobin (Cygb) is a recently discovered member of the globin family. Cygb maintains a large apolar cavity with the heme group located in it. The main catalytic activity of Cygb takes place around this heme group, which is also considered as a ligand docking station. Biochemical experiments revealed that Cygb can scavenge reactive oxygen and nitrogen species, such as hydrogen peroxide, nitric oxide, and peroxinitrite, protecting the cell against oxidative and nitrosative stress. However, the effect of mutation, as well as its synergistic effect together with oxidation on scavenging activity of the Cygb, has not yet been studied in detail. Thus, in this research we perform molecular dynamics and docking simulations to study the impact of mutation and oxidation on the Cygb catalytic function. Our simulation results show that the mutation of lysine 80 residue to alanine in Cygb results in an opening of the access to the heme group, thereby increasing its scavenging function. Moreover, the combination of this mutation (i.e., Cygb (L80A) ) with the oxidation [namely, the disulfide bond formation in Cygb (i.e., Cygb S-S )] induces the complex conformational changes in its structure. As a result, these changes lead to even more opening of the access to the heme group, which in turn enhances the scavenging activity of Cygb to a more extent. The latter can be a hallmark of enhanced enzymatic function of the modified Cygb (i.e., Cygb (L80A) and Cygb S-S ).","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67349177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1615/plasmamed.2021040971
H. Baniya, P. Khadka, S. Pandey, Anusuya Nepal, R. P. Guragain, T. R. Lamichhane, S. Dhungana, B. Shrestha, D. Subedi
{"title":"Characterization of Cold Atmospheric Pressure Plasma Technology and Its Application in Biomedicine","authors":"H. Baniya, P. Khadka, S. Pandey, Anusuya Nepal, R. P. Guragain, T. R. Lamichhane, S. Dhungana, B. Shrestha, D. Subedi","doi":"10.1615/plasmamed.2021040971","DOIUrl":"https://doi.org/10.1615/plasmamed.2021040971","url":null,"abstract":"","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67349117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1615/plasmamed.2021039616
Cian D. Madigan, Fiona O'Neill, D. O’Sullivan, Darren F. Kavanagh, Liam O'neill
{"title":"Exploring the Effects of Direct and Indirect Treatment Of Human Cells with Cold Atmospheric Plasma Devices","authors":"Cian D. Madigan, Fiona O'Neill, D. O’Sullivan, Darren F. Kavanagh, Liam O'neill","doi":"10.1615/plasmamed.2021039616","DOIUrl":"https://doi.org/10.1615/plasmamed.2021039616","url":null,"abstract":"","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1615/plasmamed.2021040968
J. Krištof, Fariha Mustafa, A. Yahaya, M. Blajan, K. Shimizu
One of the main roles of transdermal drug delivery is to overcome stratum corneum; the first barrier of the skin. We combined several types of treatments to investigate their effect on skin permeability and drug absorption. Franz diffusion cell was used to investigate the permeation and retention of adenosine through Yucatan micropig’s skin. The amount of the penetrated drug and the drug retained inside the skin was measured by HPLC. We compared adenosine permeation through the untreated skin, iontophoretically pre-treated skin with FeSO4∙7H2O in DMSO followed by microplasma treatment, iontophoretically pre-treated skin with FeSO4∙7H2O in water followed by microplasma treatment, and treated skin with DMSO. Eosin Y dye was used to analyze depth of the penetration after the treatment. Depth of penetration was displayed by microscopic observation and FE-SEM/EDS observation after skin sectioning. Absorption of eosin Y inside the skin measured by FE-SEM/EDS and optical microscope was consistent with absorption of adenosine measured by using Franz diffusion cell and HPLC. Iontophoretic pre-treatment followed by plasma treatment caused increased drug absorption in stratum corneum and plasma treatment itself increased drug penetration through the epidermal layer of the skin.
{"title":"Transdermal delivery of Adenosine and Eosin Y using microplasma combined with FeSO4 and DMSO iontophoresis","authors":"J. Krištof, Fariha Mustafa, A. Yahaya, M. Blajan, K. Shimizu","doi":"10.1615/plasmamed.2021040968","DOIUrl":"https://doi.org/10.1615/plasmamed.2021040968","url":null,"abstract":"One of the main roles of transdermal drug delivery is to overcome stratum corneum; the first barrier of the skin. We combined several types of treatments to investigate their effect on skin permeability and drug absorption. Franz diffusion cell was used to investigate the permeation and retention of adenosine through Yucatan micropig’s skin. The amount of the penetrated drug and the drug retained inside the skin was measured by HPLC. We compared adenosine permeation through the untreated skin, iontophoretically pre-treated skin with FeSO4∙7H2O in DMSO followed by microplasma treatment, iontophoretically pre-treated skin with FeSO4∙7H2O in water followed by microplasma treatment, and treated skin with DMSO. Eosin Y dye was used to analyze depth of the penetration after the treatment. Depth of penetration was displayed by microscopic observation and FE-SEM/EDS observation after skin sectioning. Absorption of eosin Y inside the skin measured by FE-SEM/EDS and optical microscope was consistent with absorption of adenosine measured by using Franz diffusion cell and HPLC. Iontophoretic pre-treatment followed by plasma treatment caused increased drug absorption in stratum corneum and plasma treatment itself increased drug penetration through the epidermal layer of the skin.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67349104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1615/plasmamed.2021041385
Tal Horovitz, Nathan Monesa, Aviel Hanasab, R. Foulad
: The use of high-frequency currents as a therapy for treating various medical conditions gained momentum during the late 19th-century but began to decline about 30 years later. Around this time various researchers discredited the reputation of high-frequency currents (HFCs) by indicating that HFCs produced their therapeutic effects through the action of heat alone, without a “specific effect” of HFCs. The rejection of the Rife machine by many physicians, due to a lack of understanding of its principles, along with the rise in fraudulent Rife machines, also added doubt to the validity of electrotherapeutics. Furthermore, the simultaneous rise in popularity of the pharmaceutical industry and X-ray therapy ensured the decline in popularity, and hence funding for the research of HFCs.
{"title":"The Decline of 20th Century High-Frequency Therapeutics","authors":"Tal Horovitz, Nathan Monesa, Aviel Hanasab, R. Foulad","doi":"10.1615/plasmamed.2021041385","DOIUrl":"https://doi.org/10.1615/plasmamed.2021041385","url":null,"abstract":": The use of high-frequency currents as a therapy for treating various medical conditions gained momentum during the late 19th-century but began to decline about 30 years later. Around this time various researchers discredited the reputation of high-frequency currents (HFCs) by indicating that HFCs produced their therapeutic effects through the action of heat alone, without a “specific effect” of HFCs. The rejection of the Rife machine by many physicians, due to a lack of understanding of its principles, along with the rise in fraudulent Rife machines, also added doubt to the validity of electrotherapeutics. Furthermore, the simultaneous rise in popularity of the pharmaceutical industry and X-ray therapy ensured the decline in popularity, and hence funding for the research of HFCs.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67349172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1615/plasmamed.2021039083
S. Darmawati, N. Nasruddin, G. S. A. Putri, A. Iswara, P. Kurniasiwi, E. S. Wahyuningtyas, L. H. Nurani, Defi Nurul Hayati, T. Ishijima, T. Nakatani, J. Sugama
{"title":"Accelerated healing of chronic wound under the combinatorial therapeutic regimen based on contact and non-contact styles of the cold atmospheric plasma jet","authors":"S. Darmawati, N. Nasruddin, G. S. A. Putri, A. Iswara, P. Kurniasiwi, E. S. Wahyuningtyas, L. H. Nurani, Defi Nurul Hayati, T. Ishijima, T. Nakatani, J. Sugama","doi":"10.1615/plasmamed.2021039083","DOIUrl":"https://doi.org/10.1615/plasmamed.2021039083","url":null,"abstract":"","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.1615/plasmamed.2021039685
L. O'Neill, B. Twomey, F. Tan, J. O'donoghue, John Junt
Surface modification of implants is now an essential aspect of biocompatibility. A single-step process to attach biomolecules to implants represents a major advance, and plasma coating potentially achieves this. An aqueous solution of collagen was sprayed into a nonthermal plasma and deposited onto titanium surfaces. The plasma instantly transformed the liquid aerosol into a coagulated dry coating on the implant surface. Surface analysis confirmed the presence of a thin, conformal protein layer on the metal surface. Titanium fixation screws were coated first with hydroxyapatite and then with a layer of collagen and were implanted into the femurs of New Zealand white rabbits and compared to various control surfaces. Measurements of the rotational torque removal force indicated that the collagen coating enhanced bone fixation and created a more reproducible well-anchored, stable implant than the traditional plasma-sprayed hydroxyapatite coatings. Nonthermal plasma processing offers a single step route to the biological functionalization of implantable surfaces. The process requires no polymers, primers, or linkers and offers an opportunity to control biocompatibility and to tailor local response to the implant in vivo. This opens the door to a wide variety of novel biological surface coatings across all aspects of life sciences and medicine.
{"title":"Collagen Coating of Titanium Implants Using Nonthermal Plasma","authors":"L. O'Neill, B. Twomey, F. Tan, J. O'donoghue, John Junt","doi":"10.1615/plasmamed.2021039685","DOIUrl":"https://doi.org/10.1615/plasmamed.2021039685","url":null,"abstract":"Surface modification of implants is now an essential aspect of biocompatibility. A single-step process to attach biomolecules to implants represents a major advance, and plasma coating potentially achieves this. An aqueous solution of collagen was sprayed into a nonthermal plasma and deposited onto titanium surfaces. The plasma instantly transformed the liquid aerosol into a coagulated dry coating on the implant surface. Surface analysis confirmed the presence of a thin, conformal protein layer on the metal surface. Titanium fixation screws were coated first with hydroxyapatite and then with a layer of collagen and were implanted into the femurs of New Zealand white rabbits and compared to various control surfaces. Measurements of the rotational torque removal force indicated that the collagen coating enhanced bone fixation and created a more reproducible well-anchored, stable implant than the traditional plasma-sprayed hydroxyapatite coatings. Nonthermal plasma processing offers a single step route to the biological functionalization of implantable surfaces. The process requires no polymers, primers, or linkers and offers an opportunity to control biocompatibility and to tailor local response to the implant in vivo. This opens the door to a wide variety of novel biological surface coatings across all aspects of life sciences and medicine.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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