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La recuperación de la naturaleza por el aislamiento social debido al Covid-19 ¿Realidad o ficción? Covid-19导致社会孤立的自然恢复:事实还是虚构?
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200060
T. M. Marín Velásquez
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引用次数: 0
Phaseolus vulgaris en el tratamiento de suelo un agrícola contaminado por hidrocarburos 菜豆在处理农业碳氢化合物污染土壤中的应用
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200094
Liliana Marques-Benavides, Diana Modesto-Sánchez, Blanca Celeste Saucedo-Martínez, J. Rico-Cerda, Lilian Bribiesca-Rodríguez, J. M. Sánchez-Yáñez
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引用次数: 0
Presencia de patógenos en carne cruda de pollo en centros de expendio, Huánuco-Perú huanuco - peru,配送中心生鸡肉中存在病原体
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200130
J. Vásquez-Ampuero, Walter Richard Tasayco-Alcántara
Juan Marco Vásquez-Ampuero E-mail address: jmarcovasqueza@gmail.com El objetivo de este estudio fue determinar la condición higiénica sanitaria de los centros de expendio de carne de pollo (CP) crudo de los mercados principales de la ciudad de Huánuco-Perú. Se muestrearon 50 establecimientos que expenden CP cruda. En cada uno de los locales se tomaron muestras de 200 g de carne, las cuales fueron procesadas en el LM-FMVZ UNHV. Para la detección de Escherichia coli, se empleó placas Petrifilm EC de acuerdo al método oficial y para Salmonella spp., el ensayo Salmonella Express System (SALX) en placas 3M Petrifilm. Paralelamente, para evaluar las condiciones higiénicas sanitarias de los establecimientos y de los responsables del expendio de carne, se aplicó un cuestionario de buenas prácticas de manipulación. Se observó que todas las muestras se encontraban contaminadas, con UFC/g PF de E. coli, en promedio 1988 UFC/g PF±3.74 de E. coli. Y en cuanto a Salmonella spp., todos los casos fueron positivos a la misma. Además, se demuestran condiciones higiénicas sanitarias deficientes y regulares de infraestructura. Por tanto, el expendio de CP cruda no cumple con las buenas prácticas de manipulación de alimentos. En los mercados de Huánuco, la CP expendida está considerada por la NTP no es apta para el consumo humano.
Juan Marco vasquez - ampuero E-mail address: jmarcovasqueza@gmail.com本研究的目的是确定huanuco - peru市主要市场的生鸡肉(CP)配送中心的卫生条件。本研究的目的是确定在墨西哥销售生CP的机构。在每个地点采集200克肉样本,并在lmfmvz UNHV进行加工。大肠杆菌的检测采用Petrifilm EC板,沙门氏菌的检测采用3M Petrifilm板沙门氏菌表达系统(SALX)。本研究的目的是评估肉类销售场所的卫生条件和管理人员的卫生条件,并采用良好处理规范问卷。所有样品均被大肠杆菌cfu /g PF污染,平均1988 cfu /g PF±3.74大肠杆菌污染。至于沙门氏菌,所有病例均为阳性。此外,基础设施的卫生条件也很差。因此,生CP的销售不符合良好的食品处理规范。在huanuco市场,NTP认为发放的CP不适合人类消费。
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引用次数: 1
The recovery of nature through social isolation by Covid-19 ¿Reality or fiction? 通过Covid-19的社会隔离来恢复自然:现实还是虚构?
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200060x
Tomás Darío Marín Velásquez
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引用次数: 0
Description and evaluation of the effects of the cetogenic diet in children with refractory epilepsy 致痫饮食对难治性癫痫患儿疗效的描述与评价
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200142x
A. Rodríguez-Hernández, Mónica Elizabeth Pelayo-González
J. Selva Andina Res. Soc. 2020; 11(2):142-152. In Refractory epilepsy is due to that drug-resistant epilepsy in which there is no improvement after the application of various antiepileptic drugs (AEDs). The Telethon Children's Rehabilitation Center cares for a large population of children with some type of epilepsy. Recently, the application of the home intervention protocol for the ketogenic diet (DC) has increased. A few years ago, DC was only attended with extreme surveillance in a hospital center under rigid schemes. With this background, the objective of this study was to describe the experience and findings of a series of 10 cases of children with RE treated with this DC protocol over the course of at least 1 year. All the cases of children (n = 10) attended at the TCRC, Aguascalientes, Mexico, who were treated with the ketogenic diet between the dates of February 2010 and June 2015, were reviewed by means of an electronic clinical record criteria for refractory epilepsy, as well as surveillance for pediatric neurology and nutrition. The DC home intervention protocol was established under the criteria of Lambruschini-Nilo & Gutiérrez-Sánchez, where it started with a classic type DC with a 2: 1 ratio during the first week of adaptation and then went on to the 3: 1 or 4 ratio: 1 corresponding according to patient tolerance. The reduction in the number of crises was measured at 3 months and one year after surgery. A one-way ANOVA general linear model with repeated measures was applied to measure the efficacy at 3 months and one year of treatment, finding statistical significance when comparing the results means of The ID of artícle: 134/JSARS/2019 Record from the article. Received November 2019. Returned February 2020. Accepted April 2020. Available online, August 2020. Edited by: Selva Andina Research Society
J.Selva Andina Res.Soc.2020;11(2):142-152.难治性癫痫是由于应用各种抗癫痫药物(AED)后没有改善的耐药性癫痫。Telethon儿童康复中心照顾大量患有某种类型癫痫的儿童。最近,家庭干预方案在生酮饮食(DC)中的应用有所增加。几年前,DC只是在严格的计划下,在医院中心接受极端监视。在这种背景下,本研究的目的是描述一系列10例RE儿童在至少1年的时间里接受该DC方案治疗的经验和发现。通过难治性癫痫的电子临床记录标准以及儿科神经病学和营养监测,对2010年2月至2015年6月期间在墨西哥阿瓜斯卡连特斯TCRC接受生酮饮食治疗的所有儿童病例(n=10)进行了审查。DC家庭干预方案是根据Lambruschini Nilo&Gutiérrez-Sánchez的标准制定的,在适应的第一周,它从2∶1的经典型DC开始,然后根据患者的耐受性,采用3∶1或4∶1的比例。在手术后3个月和1年测量了危机次数的减少。采用具有重复测量的单因素方差分析通用线性模型来测量治疗3个月和1年的疗效,在比较文章中的ID of artícle:134/JSARS/2019记录的结果时发现具有统计学意义。收到日期:2019年11月。2020年2月返回。2020年4月接受。在线提供,2020年8月。编辑:Selva Andina研究会
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引用次数: 0
Asociación del polimorfismo genético del locus HLA-G y la susceptibilidad a contraer lupus eritematoso sistémico expresada en algunas manifestaciones clínicas HLA-G基因多态性与系统性红斑狼疮易感性的关系表现为某些临床表现
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200062
Gabriela Guerra-Monrroy, Luis Fernando Sosa-Tordoya
J. Selva Andina Res. Soc. 2020; 11(2):62-74. In Autoimmune diseases such as systemic lupus erythematosus (SLE) are characterized by inflammation, as well as the progressive development of antibodies and T lymphocytes directed against self antigens. Although the etiology is still unknown, it is known that there is a strong genetic association between these diseases and some alleles and / or haplotypes of the major histocompatibility complex (MHC). Individual susceptibility in autoimmunity may be determined by a combination of gene-specific polymorphisms that code for multiple proteins, cytokines, adhesion molecules, among others, and that is why it is important to study them. HLA-G is an MHC class I glycoprotein molecule, which performs very important functions when activating and The ID of artícle: 136/JSARS/2020 Guerra-Monrroy & Sosa-Tordoya J. Selva Andina Res. Soc. ______________________________________________________________________________________________________________ 63 Record from the article. Received march 2020. Returned may 2020. Accepted june 2020. Available online, august 2020. regulating the immune system. Therefore, what this study intends is to determine the genetic association between the 14 bp polymorphism of the HLA-G gene with the susceptibility to contract SLE and the clinical manifestations of the disease. The study population consisted of 120 patients with SLE and 112 patients without the disease. For the study, human DNA was obtained from peripheral blood, PCR was performed for molecular typing of the genotypes and alleles that were revealed by means of electrophoresis. in agarose gel. At the same time, serological tests were carried out using the ELISA technique to determine the presence of double-chain anti-.DNA IgG antibodies. The PCR results showed that lupus patients have a higher frequency of expression of the Ins/Del genotype (OR = 1.72, p <0.05); while, the presence of the homozygous Ins/Ins genotype is more frequent in the control group (OR = 0.29, p <0.001), thus showing that the first genotype is a risk factor and the second, a protection factor to suffer SLE respectively. It was also observed that between patients and controls there is no significant difference in the frequency of presentation of the Del/Del genotype in homozygosity. Regarding allele frequencies, the deletion allele was found to be more frequent in the group of lupus patients, compared to the control group where both alleles presented the same percentage. Regarding the clinical manifestations, it was observed that the Ins/Del polymorphism (OR = 8.64) is a risk factor for the development of dermatological manifestations. 2020. Journal of the Selva Andina Research Society®. Bolivia. All rights reserved. Edited by: Selva Andina Research Society
J. Selva Andina Res. Soc. 2020;11(2): 62 - 74。自身免疫性疾病,如系统性红斑狼疮(SLE),其特征是炎症,以及针对自身抗原的抗体和T淋巴细胞的进行性发展。虽然病因尚不清楚,但已知这些疾病与主要组织相容性复合体(MHC)的一些等位基因和/或单倍型之间存在很强的遗传关联。自身免疫的个体易感性可能是由基因特异性多态性的组合决定的,这些多态性编码多种蛋白质、细胞因子、粘附分子等,这就是研究它们的重要性。HLA-G是一种MHC I类糖蛋白分子,它在激活和激活MHC时起着非常重要的作用。______________________________________________________________________________________________________________ 从这篇文章63记录。2020年3月收。2020年5月返回。2020年6月接受。2020年8月在线发售。调节免疫系统。因此,本研究旨在确定与SLE易感性相关的HLA-G基因14bp多态性与SLE临床表现之间的遗传关系。研究人群包括120例SLE患者和112例无SLE患者。本研究从人外周血中提取DNA,用PCR方法对电泳所得基因型和等位基因进行分子分型。琼脂糖凝胶。同时采用ELISA技术进行血清学检测,确定双链抗-的存在。DNA IgG抗体。PCR结果显示,狼疮患者Ins/Del基因型的表达频率较高(OR = 1.72, p <0.05);而Ins/Ins纯合子基因型在对照组中更为常见(OR = 0.29, p <0.001),说明Ins/Ins纯合子基因型是SLE的危险因素,Ins/Ins纯合子基因型是SLE的保护因素。还观察到,在患者和对照组之间,Del/Del基因型纯合性的出现频率没有显著差异。在等位基因频率方面,与对照组相比,缺失等位基因在狼疮患者组中的频率更高,而对照组中两种等位基因的比例相同。在临床表现方面,Ins/Del多态性(OR = 8.64)是发生皮肤病表现的危险因素。2020. Selva Andina研究学会杂志。玻利维亚。版权所有。编辑:塞尔瓦·安迪纳研究协会
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引用次数: 0
Calidad nutricional y niveles de aceptabilidad de productos innovados con base a pescado: empanizados y kamaboko 以鱼为基础的创新产品的营养质量和可接受性水平:Enpanizado和Kamaboko
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200153
Vilma Amalia Vilca-Cáceres, Norka Lisett Gómez-Pacco, Wendy Del Rosario Vargas Callo
Vilma Amalia Vilca-Cáceres E-mail address: vilcacaceresv@gmail.com El presente estudio tiene como objetivo primordial evaluar la calidad nutricional y los niveles de aceptabilidad de productos innovados con base a pescado en la Provincia de Llo, Perú. La investigación fue de tipo aplicada. La elaboración de los productos se realizó en el laboratorio de procesos pesqueros de la Escuela Profesional de Ingeniería Pesquera de la Universidad Nacional de Moquegua, luego se llevó a cabo la evaluación nutricional en el laboratorio de la Facultad de Zootecnia de la Universidad Nacional Agraria la Molina y posteriormente se efectuó el análisis de aceptabilidad en las instalaciones del comedor de la Institución Educativa Santa Elizabeth de la Provincia de Llo, utilizándose como instrumento una escala hedónica de categorías de 7 niveles. Los resultados arrojan que la proteína en los productos innovados: empanizados y kamaboko (pasta de pescado) están presente con el 25.55 % en el empanizado con harina de habas, seguido de empanizado con harina de plátano con 21.94 % y finalmente el kamaboko con 20.34%. El empanizado con harina de plátano fue el que tuvo mayor aceptación con un 86.3 %, seguido de la pasta de kamaboko con un 59.2 %, mientras que el empanizado con harina de habas solo obtuvo un 26.7 %. Al evaluar los niveles de calidad nutricional y de aceptabilidad de los productos innovados con base a pescado, se puede concluir que, tienen calidad nutricional y un nivel alto de aceptación, especialmente el empanizado con harina de plátano y la pasta del kamaboko.
Vilma Amalia vilca - caceres E-mail: vilcacaceresv@gmail.com本研究的主要目的是评估秘鲁洛省创新鱼类产品的营养质量和可接受水平。这项研究是应用型的。制定渔业产品实验室进行工艺工程专业学校Moquegua国立大学渔业,然后进行营养评估实验室在国立大学动物学系土地Molina和其后进行分析接受在餐厅设施所在教育机构Llo省的圣伊丽莎白使用7级享乐量表作为工具。结果表明,豆粉面包和kamaboko(鱼酱)中蛋白质含量为25.55%,香蕉粉面包中蛋白质含量为21.94%,最后kamaboko面包中蛋白质含量为20.34%。香蕉粉面包屑的接受度最高,为86.3%,其次是kamaboko意大利面,为59.2%,豆粉面包屑的接受度仅为26.7%。通过评估以鱼为基础的创新产品的营养质量和可接受性水平,可以得出结论,它们具有较高的营养质量和可接受性,特别是香蕉粉面包和kamaboko面食。
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引用次数: 1
Association of the HLA-G locus genetic polymorphism and the susceptibility to contract systemic lupus erythematosus expressed in some clinical manifestations HLA-G基因座多态性与系统性红斑狼疮易感性的关系
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200062x
Gabriela Guerra-Monrroy, Luis Fernando Sosa-Tordoya
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引用次数: 1
Recuperación de un suelo contaminado por una mezcla de hidrocarburos 修复被碳氢化合物混合物污染的土壤
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-08-01 DOI: 10.36610/j.jsars.2020.110200075
J. Rico-Cerda, Juan Luis Ignacio-De la Cruz, Perla Guadalupe Mondragón-Reynel, J. M. Sánchez-Yáñez
Juna Manuel Sánchez-Yáñez E-mail address: syanez@umich.mx En México, un suelo agrícola pobre en nitrógeno (N) contaminado por un derivado de hidrocarburos como el aceite residual automotriz (ARA), con una relativa alta concentración de 100000 ppm, es un problema ambiental, pero también porque afecta drásticamente las propiedades del suelo asociadas con la mineralización de la materia orgánica y la pérdida de fertilidad, ya que sobrepasa el límite máximo aceptado de 4400 ppm de la norma mexicana llamada, NOM-138-SEMARNAT-2012 (NOM-138). Una alternativa de solución es tratarlo con acciones ecológicas para eliminar el ARA y recuperar esa fertilidad. Por lo que los objetivos de esta investigación fueron: i) biorremediación de suelo contaminado por 100000 ppm de ARA ii) fitorremediación mediante Sorghum vulgare con Aspergillus niger y Penicillium chrysogenum para decrecer el ARA a un valor inferior a 4400 ppm de la NOM-138. Para ello la recuperación de suelo se realizó mediante la variable-respuesta: desaparición del ARA por Soxhlet al inicio y después de la biorremediación y al final de la fitorremediación con S. vulgare con la fenología y biomasa a plántula. Todos los datos experimentales se validaron por ANOVA/Tukey HSD P<0.05%. Los resultados indicaron que la biorremediación y la fitorremediación del suelo contaminado por 100000 ppm de ARA, la decrecieron hasta 3400 ppm, valor inferior al máximo establecido por la NOM-138, suficiente para la recuperación del suelo en la producción agrícola, en 120 días un periodo de tiempo relativamente corto.
朱娜·曼努埃尔·桑切斯-亚涅斯电子邮件地址:syanez@umich.mx在墨西哥,受汽车渣油(ARA)等碳氢化合物衍生物污染的氮(N)贫瘠的农业土壤是一个环境问题,相对浓度高达10万ppm,但也因为它严重影响了与有机物矿化和肥力丧失有关的土壤特性,因为它超过了墨西哥标准NOM-138-SEMARNAT-2012(NOM-138)中4400 ppm的可接受上限。另一种解决方案是通过生态行动来处理它,以消除ARA并恢复这种肥力。因此,本研究的目的是:(i)对受10万ppm ARB污染的土壤进行生物修复;(ii)用黑曲霉和产黄青霉对高粱进行植物修复,将ARA降至低于4400 ppm的NOM-138。为此,土壤恢复是通过变量-响应进行的:在生物修复开始和之后,以及在植物修复结束时,通过Soxhlet清除ARA。用物候学和幼苗生物量进行普通。所有实验数据都经过ANOVA/Tukey-HSP<0.05%的验证。结果表明,受10万ppm ARA污染的土壤的生物修复和植物修复使其降至3400 ppm,低于NOM-138规定的最大值,足以在120天内恢复农业生产中的土壤,时间相对较短。
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引用次数: 1
Determinación de glucosa 葡萄糖测定
IF 0.1 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-02-01 DOI: 10.36610/j.jsars.2020.110100038
Peggy Brenda Gygliola-Ormachea, Gabriela Tarquino-Flores, Edgar Chambi-Gutierrez, Kattia Averanga-Conde, Lily Salcedo-Ortiz
J. Selva Andina Res. Soc. 2020; 11(1):38-48. In The objective of this study was to determine the usefulness of glucometers in relation to the standard laboratory method (MEL) in blood samples: healthy, diabetic, erythrocytic and anemic. The glucometers (GM) evaluated were ACCU CHEK Active in its two procedures (ACCU I and ACCU II), ONE TOUCH Ultra (ONETu) and Easy TRUEread (TRUEf). The ANOVA analysis of variance, p<0.005, was used. Of 54 healthy samples with hematocrit 49±4, ONETu did not show a significant difference with MEL, ACCU I, ACCU II and TRUEf showed significant differences, being TRUEf the one that revealed the greatest difference with over-estimated values in more than 30 mg / dL. Of 60 diabetic samples with hematocrit 48 ± 8, there were no significant differences between GM and MEL, however incongruous results were detected 2 to 3 times overestimated (13%) and 2 to 3 times undervalued (12%) with GM ONETu and TRUEf; ACCU I and ACCU II exposed slight inconsistencies. Of 54 erythrocyte samples with hematocrit 66 ± 6, underestimated values were detected in 20 mg / dL with ONETu and TRUEf (10%), ACCU I (18%); ACCU II reported overestimated values between 15-25 mg / dL (40%) with significant statistical difference. Of 40 anemic samples with hematocrit 29 ± 9, overestimated values were reported, 20% (ONETu), 35% (TRUEf), 45% (ACCU II) and 55% (ACCU I), with a difference greater than 20 mg / dL. The GM evaluated present different degrees of interference by diabetic, anemic and erythrocytic samples, in the case of erythrocytic samples, undervalued values are observed, in anemic samples overvalued values, these data should be considered for a better use of GM. © 2020. Journal of the Selva Andina Research Society. Bolivia. All rights reserved. Historial del artículo. Recibido octubre 2019. Devuelto noviembre 2019. Aceptado diciembre 2019. Disponible en línea, febrero 2020. Editado por: Selva Andina Research Society
J. Selva Andina Res. Soc. 2020;38 - 48。11 (1):本研究的目的是确定血糖仪与标准实验室方法(MEL)在血液样本中的有用性:健康、糖尿病、红细胞和贫血。评估的血糖仪(GM)在其两个程序(ACCU I和ACCU II)中为ACCU check Active, ONE TOUCH Ultra (ONETu)和Easy TRUEread (TRUEf)。采用方差分析,p<0.005。在54例红细胞比容为49±4的健康样本中,ONETu与MEL无显著差异,ACCU I、ACCU II和TRUEf有显著差异,其中TRUEf与30 mg / dL以上的高估值差异最大。在60例红细胞压积为48±8的糖尿病样本中,GM和MEL之间无显著差异,但GM ONETu和TRUEf的结果不一致,分别被高估2 ~ 3倍(13%)和低估2 ~ 3倍(12%);ACCU I和ACCU II显示出轻微的不一致。在54份红细胞比容为66±6的红细胞样本中,ONETu和TRUEf在20 mg / dL时检测到低估值(10%),ACCU I (18%);ACCU II报告的高估值在15 ~ 25 mg / dL之间(40%),差异有统计学意义。在40份红细胞比容为29±9的贫血样本中,报告的高估值为20% (ONETu), 35% (TRUEf), 45% (ACCU II)和55% (ACCU I),差异大于20 mg / dL。所评估的转基因对糖尿病、贫血和红细胞样本存在不同程度的干扰,在红细胞样本中观察到低估值,在贫血样本中观察到高估值,这些数据应被考虑以更好地使用转基因。©2020。塞尔瓦·安迪纳研究学会杂志。玻利维亚。版权所有。历史del artículo。2019年10月。Devuelto 2019年11月。接受2019年。一次性材料línea, 2020年2月。Editado poror: Selva Andina研究协会
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引用次数: 0
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Journal of the Selva Andina Research Society
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