Pub Date : 2020-02-01DOI: 10.36610/j.jsars.2020.110100057
C. A. R. Campos
{"title":"Interculturalidad y universidad","authors":"C. A. R. Campos","doi":"10.36610/j.jsars.2020.110100057","DOIUrl":"https://doi.org/10.36610/j.jsars.2020.110100057","url":null,"abstract":"","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":"11 1","pages":"57-59"},"PeriodicalIF":0.1,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44677957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.36610/j.jsars.2020.110100029
Maritza Rodríguez-Lizana, Walter Wilfredo Ochoa-Yupanqui
Walter Wilfredo Ochoa-Yupanqui E-mail address: wwalter8a@hotmail.com La investigación tuvo como objetivo, determinar la actividad biocida del extracto crudo etanólico del Piper elongatum (Matico) de cervicitis en mujeres en edad fértil, de una población fue de 250 mujeres en edad fértil, 50 mujeres con diagnóstico de cervicitis aguda (34) y crónica (16), con edad promedio de 38 años, conformando grupos control y caso por 25 mujeres. Como instrumento se utilizó la hoja de entrevista estructurada, resultados de PAP y resultados de exámenes de laboratorio. Se lograron resultados alentadores 29.4% de las mujeres con cervicitis aguda recibieron el tratamiento con extracto crudo etanólico de matico al 1% se aliviaron frente al 20.6% del grupo control (X: 4.37, p=0.037), mientras las mujeres diagnosticadas con cervicitis crónica, tratadas con extracto crudo etanólico al 5%, se aliviaron el 50% y ninguna en el grupo control, lo que indica que hay efectividad del extracto crudo etanólico de matico (X: 7.27, p=0.007). Se concluye que los componentes bioactivos presentes en el extracto crudo etanólico tienen actividad biocida sobre la cervicitis aguda y crónica.
Walter Wilfredo Ochoa-Yupanqui电子邮件地址：wwalter8a@hotmail.com这项研究的目的是确定长胡椒粗乙醇提取物（MATICO）对育龄妇女宫颈炎的杀生物活性，其中250名育龄妇女、50名确诊为急性（34名）和慢性（16名）宫颈炎的妇女，平均年龄为38岁，组成对照组，每25名妇女各1例。使用结构化面试表、PAP结果和实验室检查结果作为工具。结果令人鼓舞的是，29.4%的急性宫颈炎妇女接受了1%的Matico粗乙醇提取物治疗，缓解了20.6%的对照组（x:4.37，p=0.037），而诊断患有慢性宫颈炎的妇女接受了5%的粗乙醇提取物治疗，缓解了50%，对照组没有缓解，这表明Matico的粗乙醇提取物是有效的（x:7.27，p=0.007）。得出的结论是，粗乙醇提取物中存在的生物活性成分对急慢性宫颈炎具有杀菌活性。
{"title":"Actividad biocida del extracto crudo etanólico crudo del Piper elongatum “Matico” en cervicitis en mujeres en edad fértil, como alternativa terapéutica","authors":"Maritza Rodríguez-Lizana, Walter Wilfredo Ochoa-Yupanqui","doi":"10.36610/j.jsars.2020.110100029","DOIUrl":"https://doi.org/10.36610/j.jsars.2020.110100029","url":null,"abstract":"Walter Wilfredo Ochoa-Yupanqui E-mail address: wwalter8a@hotmail.com La investigación tuvo como objetivo, determinar la actividad biocida del extracto crudo etanólico del Piper elongatum (Matico) de cervicitis en mujeres en edad fértil, de una población fue de 250 mujeres en edad fértil, 50 mujeres con diagnóstico de cervicitis aguda (34) y crónica (16), con edad promedio de 38 años, conformando grupos control y caso por 25 mujeres. Como instrumento se utilizó la hoja de entrevista estructurada, resultados de PAP y resultados de exámenes de laboratorio. Se lograron resultados alentadores 29.4% de las mujeres con cervicitis aguda recibieron el tratamiento con extracto crudo etanólico de matico al 1% se aliviaron frente al 20.6% del grupo control (X: 4.37, p=0.037), mientras las mujeres diagnosticadas con cervicitis crónica, tratadas con extracto crudo etanólico al 5%, se aliviaron el 50% y ninguna en el grupo control, lo que indica que hay efectividad del extracto crudo etanólico de matico (X: 7.27, p=0.007). Se concluye que los componentes bioactivos presentes en el extracto crudo etanólico tienen actividad biocida sobre la cervicitis aguda y crónica.","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":"11 1","pages":"29-37"},"PeriodicalIF":0.1,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42132118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.36610/j.jsars.2020.110100002
Julio Gabriel-Ortega, Juan Delvalle-García, Jaime Padilla-Piloso, Nixon Pincay-Quijije, Fernando Ayón-Villao, Washington Narváez-Campana, Alfredo González-Vázquez
Between 2017 and 2018, a project on the development of technological alternatives for the sustainable production of greenhouse vegetables was implemented, with the aim of innovating the change in the horticultural production matrix to contribute to the reduction of the effect of climate change on the Puerto La Boca area, Jipijapa, Ecuador. In order to fulfill this objective, various activities were developed, articulating the three substantive axes of the State University of the South of Manabi, such as academia, research and linking, with the participation of the same university, the Agro-artisan Association of Puerto la Boca, the Decentralized Autonomous Government (DAG Municipal) and the private company. The substantive functions were articulated through the development of knowledge and technologies, the strengthening of the capacities of the producers, the realization of field days, as well as the systematic involvement of students (in research, pre-professional practices and bonding) and teachers in the activities The results achieved were the development of a technological offer such as tomato (Alambra, Itaipu and Baikor), pepper (Macantro and Tandara) and melon (Kapaz and Kazik) hybrids and methods [Integrated disease management (IDM) and management integrated vegetables (MIV)] more appropriate and timely as the development of a strategy based on four fundamental principles such as: a) The alternation of systemic and contact fungicides, b) non-application of the systemic fungicide more than three times c) the use of microorganisms (Bacillus and/or Trichoderma) that replace the contact fungicide and d) the reduction in the number of applications. These technologies and methodologies were made available to producers, so that they achieve better products and give them the opportunity to achieve business with better profitability margins in differentiated market niches (supermarkets); as well as the strengthening of the knowledge, attitudes and practices of the producers
{"title":"Innovaciones en la matriz productiva hortícola para reducir el efecto del cambio climático en Puerto la Boca, Jipijapa, Ecuador","authors":"Julio Gabriel-Ortega, Juan Delvalle-García, Jaime Padilla-Piloso, Nixon Pincay-Quijije, Fernando Ayón-Villao, Washington Narváez-Campana, Alfredo González-Vázquez","doi":"10.36610/j.jsars.2020.110100002","DOIUrl":"https://doi.org/10.36610/j.jsars.2020.110100002","url":null,"abstract":"Between 2017 and 2018, a project on the development of technological alternatives for the sustainable production of greenhouse vegetables was implemented, with the aim of innovating the change in the horticultural production matrix to contribute to the reduction of the effect of climate change on the Puerto La Boca area, Jipijapa, Ecuador. In order to fulfill this objective, various activities were developed, articulating the three substantive axes of the State University of the South of Manabi, such as academia, research and linking, with the participation of the same university, the Agro-artisan Association of Puerto la Boca, the Decentralized Autonomous Government (DAG Municipal) and the private company. The substantive functions were articulated through the development of knowledge and technologies, the strengthening of the capacities of the producers, the realization of field days, as well as the systematic involvement of students (in research, pre-professional practices and bonding) and teachers in the activities The results achieved were the development of a technological offer such as tomato (Alambra, Itaipu and Baikor), pepper (Macantro and Tandara) and melon (Kapaz and Kazik) hybrids and methods [Integrated disease management (IDM) and management integrated vegetables (MIV)] more appropriate and timely as the development of a strategy based on four fundamental principles such as: a) The alternation of systemic and contact fungicides, b) non-application of the systemic fungicide more than three times c) the use of microorganisms (Bacillus and/or Trichoderma) that replace the contact fungicide and d) the reduction in the number of applications. These technologies and methodologies were made available to producers, so that they achieve better products and give them the opportunity to achieve business with better profitability margins in differentiated market niches (supermarkets); as well as the strengthening of the knowledge, attitudes and practices of the producers","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":"11 1","pages":"2-17"},"PeriodicalIF":0.1,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49403506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.36610/j.jsars.2020.110100049
Jorge Alberto Guillen-Martín del Campo, Antonio Calvillo-Femat, Joyce Ivonne Mosqueda-Esparza, A. I. Rodríguez-Hernández, Francisco Jaramillo-González
Recently spirulina algae have been used for weight reduction. It is known that at least 36 g of this algae provide all the essential amino acids, among other different compounds such as fiber and flavonoids with the possibility of treating obesity or overweight, The objective was to determine if the administration of spirulina helps reduce body weight in Wistar rats as well as determining possible effects on the metabolism of different general bio-chemical markers such as a lipid profile, urea and creatinine. A double-blind experimental study was applied using 10 Wistar rats, four rats called experimental rats, 5.8 mg of spirulina diluted in 1 mL of water, by orogas-tric tube for eight weeks, were administered to the control group (6 rats same species and initial weight) He was given only 1 mL of water. The weight of the experimental rats versus the controls had no significant changes. Regarding the biochemical markers, only a significant increase in the values of urea, total cholesterol, and tri-glycerides of the experimental group in relation to the control was found. The findings suggest the intake of spirulina for weight control and in possible states of malnutrition.
{"title":"Espirulina un suplemento alimenticio como posible alternativa en el control de peso. Un estudio con ratas Wistar","authors":"Jorge Alberto Guillen-Martín del Campo, Antonio Calvillo-Femat, Joyce Ivonne Mosqueda-Esparza, A. I. Rodríguez-Hernández, Francisco Jaramillo-González","doi":"10.36610/j.jsars.2020.110100049","DOIUrl":"https://doi.org/10.36610/j.jsars.2020.110100049","url":null,"abstract":"Recently spirulina algae have been used for weight reduction. It is known that at least 36 g of this algae provide all the essential amino acids, among other different compounds such as fiber and flavonoids with the possibility of treating obesity or overweight, The objective was to determine if the administration of spirulina helps reduce body weight in Wistar rats as well as determining possible effects on the metabolism of different general bio-chemical markers such as a lipid profile, urea and creatinine. A double-blind experimental study was applied using 10 Wistar rats, four rats called experimental rats, 5.8 mg of spirulina diluted in 1 mL of water, by orogas-tric tube for eight weeks, were administered to the control group (6 rats same species and initial weight) He was given only 1 mL of water. The weight of the experimental rats versus the controls had no significant changes. Regarding the biochemical markers, only a significant increase in the values of urea, total cholesterol, and tri-glycerides of the experimental group in relation to the control was found. The findings suggest the intake of spirulina for weight control and in possible states of malnutrition.","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":"11 1","pages":"49-56"},"PeriodicalIF":0.1,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49108769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.36610/j.jsars.2020.110100001
M. Loza-Murguia
{"title":"La necesidad de investigar, redactar y publicar actualidades relevantes","authors":"M. Loza-Murguia","doi":"10.36610/j.jsars.2020.110100001","DOIUrl":"https://doi.org/10.36610/j.jsars.2020.110100001","url":null,"abstract":"","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":"11 1","pages":"1-1"},"PeriodicalIF":0.1,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47985098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.36610/j.jsars.2019.100200073
Miriam Judith García-Reyna, Gustavo Santoyo-Pizano, J. L. Hernández-Mendoza, J. Cruz, J. M. Sánchez-Yáñez
Zea mays var mexicana or teocinte is a grass, considered one of the main ancestors of Zea mays. Teocinte, like other grasses, is associated with genera of endophytic plant growth promoters (EPGPB) in the interior of plant tissues. Z. mays is a domestic grass normally cultivated under the intensive production system, with relatively high doses of nitrogen fertilizer (NIFE), a possible alternative solution to minimize this problem, is the inoculation of the seed of Z. mays with genera of EPGPB teocinte, which colonize the roots, stems, leaves and promote a healthy growth at a reduced dose up to 50% NIFE such as urea, which is equivalent to decrease from 280 kg/ha-1 to 140 kg/ha-1, of what is recommended for the region of northern Michoacan and southern Guanajuato, Mexico. The objectives of this work were: i) to determine the density of Burkholderia spp BEPCV of teocinte, ii) to analyze the effect of the inoculation of Burkholderia spp EPGPB on the growth of Z. mays, at reduced dose of NIFE in greenhouse, iii) identify Burkholderia spp BEPCV teocinte. The population density of Burkholderia spp EPGPB was determined in tissues (organs), seeds, stem-leaves and roots of teocinte by sowing them on Pseudomonas cepacia azelaic acid tryptamine (PCAT), after incubation. Burkholderia spp colonies were selected for abundance in: leaves, stem, roots and/or seeds. These isolates of Burkholderia spp, were coded with the keys T41 and T42 and inoculated in Z. mays sown in sterile sand, where as the only variable-response the total dry weight was determined compared to the Z. mays cultivated with the recommended NIFE dose, for that region, it was concluded with the biochemical characterization. The results show that in Burkholderia spp BEPCV of teocinte, a density of 3.4 Log CFU/g of fresh weight (GPF) was registered in the root, of 3.0 Log CFU/GPF in the seed and a similar value in the stem-leaves of teocinte The response of Z. mays to Burkholderia T41 caused an increase of 47% in the dry weight of the root to the tillering, and of 57% to the extension of the stem, compared with the Z. mays without inoculating with the maximum level of NIFE (urea) 280 kg/ha-1. The biochemical identification profile of Burkholderia spp supports a genetic relationship with B. vietnamiensis. It is concluded that teocinte is a source of EPGPB for sustainable production Z. mays at a reduced dose of NIFE.
{"title":"Respuesta de Zea mays a Burkholderia spp endófita de Zea mays var mexicana (teocintle)","authors":"Miriam Judith García-Reyna, Gustavo Santoyo-Pizano, J. L. Hernández-Mendoza, J. Cruz, J. M. Sánchez-Yáñez","doi":"10.36610/j.jsars.2019.100200073","DOIUrl":"https://doi.org/10.36610/j.jsars.2019.100200073","url":null,"abstract":"Zea mays var mexicana or teocinte is a grass, considered one of the main ancestors of Zea mays. Teocinte, like other grasses, is associated with genera of endophytic plant growth promoters (EPGPB) in the interior of plant tissues. Z. mays is a domestic grass normally cultivated under the intensive production system, with relatively high doses of nitrogen fertilizer (NIFE), a possible alternative solution to minimize this problem, is the inoculation of the seed of Z. mays with genera of EPGPB teocinte, which colonize the roots, stems, leaves and promote a healthy growth at a reduced dose up to 50% NIFE such as urea, which is equivalent to decrease from 280 kg/ha-1 to 140 kg/ha-1, of what is recommended for the region of northern Michoacan and southern Guanajuato, Mexico. The objectives of this work were: i) to determine the density of Burkholderia spp BEPCV of teocinte, ii) to analyze the effect of the inoculation of Burkholderia spp EPGPB on the growth of Z. mays, at reduced dose of NIFE in greenhouse, iii) identify Burkholderia spp BEPCV teocinte. The population density of Burkholderia spp EPGPB was determined in tissues (organs), seeds, stem-leaves and roots of teocinte by sowing them on Pseudomonas cepacia azelaic acid tryptamine (PCAT), after incubation. Burkholderia spp colonies were selected for abundance in: leaves, stem, roots and/or seeds. These isolates of Burkholderia spp, were coded with the keys T41 and T42 and inoculated in Z. mays sown in sterile sand, where as the only variable-response the total dry weight was determined compared to the Z. mays cultivated with the recommended NIFE dose, for that region, it was concluded with the biochemical characterization. The results show that in Burkholderia spp BEPCV of teocinte, a density of 3.4 Log CFU/g of fresh weight (GPF) was registered in the root, of 3.0 Log CFU/GPF in the seed and a similar value in the stem-leaves of teocinte The response of Z. mays to Burkholderia T41 caused an increase of 47% in the dry weight of the root to the tillering, and of 57% to the extension of the stem, compared with the Z. mays without inoculating with the maximum level of NIFE (urea) 280 kg/ha-1. The biochemical identification profile of Burkholderia spp supports a genetic relationship with B. vietnamiensis. It is concluded that teocinte is a source of EPGPB for sustainable production Z. mays at a reduced dose of NIFE.","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46243194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.36610/j.jsars.2019.100200071
M. Loza-Murguia
{"title":"La comunicación y la información científica la piedra fundamental de una sociedad","authors":"M. Loza-Murguia","doi":"10.36610/j.jsars.2019.100200071","DOIUrl":"https://doi.org/10.36610/j.jsars.2019.100200071","url":null,"abstract":"","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49345716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.36610/J.JSARS.2019.100200086
J. Cruz, L. Márquez-Benavides, J. Rico-Cerda, Blanca Celeste Saucedo-Martínez, J. M. Sánchez-Yáñez
In Mexico any soil polluted by 90000 ppm waste motor oil (WMO), this concentration is over the maximun acepted of 4400 ppm by regulation law called as a NOM-138-SEMARNAT / SSA1-2003 (NOM-138), 9000 ppm of WMO is causing soil`s fertility decreasing. An alternative solution is biostimulation (BIS) by detergent following by mineral solution then. Subsequently the BIS by H2O2 as a supplier of O2 and a crude fungi extract containing laccase able to hydrolyze aromatic of WMO, then by Phaseolus vulgaris as a green manure to reduce WMO; concluding by phytoremediation (PHYTO) with Cicer arietinum The objectives of this research were: i) BIS of soil contaminated by 90000 ppm of WMO ii) PHYTO by C. arietinum with Micromonospora echinospora and Penicillium chrysogenum to decrease WMO at lower concentration value than the maximum accepted by NOM-138. In sense at soil`s, variable-response of BIS was initial and final concentration of WMO by Soxhlet, at the PHYTO, phenology and biomass of C. arietinum were taken at seedling. The experimental data were analyzed by ANOVA/Tukey HSD P <0.05%. Results showed that BIS and PHYTO of soil impacted by 90000 ppm of WMO decreased until 1200 ppm numerical value statistically different compared to 79000 ppm of soil polluted by WMO, without BIS and either PHYTO or negative control. Those results showed that BIS and PHYTO are an effective technique for recovering soil polluting by relative high level of WMO according to NOM-138.
{"title":"Suelo impactado por 90000 ppm de aceite residual automotriz: bioestimulación y fitorremediación","authors":"J. Cruz, L. Márquez-Benavides, J. Rico-Cerda, Blanca Celeste Saucedo-Martínez, J. M. Sánchez-Yáñez","doi":"10.36610/J.JSARS.2019.100200086","DOIUrl":"https://doi.org/10.36610/J.JSARS.2019.100200086","url":null,"abstract":"In Mexico any soil polluted by 90000 ppm waste motor oil (WMO), this concentration is over the maximun acepted of 4400 ppm by regulation law called as a NOM-138-SEMARNAT / SSA1-2003 (NOM-138), 9000 ppm of WMO is causing soil`s fertility decreasing. An alternative solution is biostimulation (BIS) by detergent following by mineral solution then. Subsequently the BIS by H2O2 as a supplier of O2 and a crude fungi extract containing laccase able to hydrolyze aromatic of WMO, then by Phaseolus vulgaris as a green manure to reduce WMO; concluding by phytoremediation (PHYTO) with Cicer arietinum The objectives of this research were: i) BIS of soil contaminated by 90000 ppm of WMO ii) PHYTO by C. arietinum with Micromonospora echinospora and Penicillium chrysogenum to decrease WMO at lower concentration value than the maximum accepted by NOM-138. In sense at soil`s, variable-response of BIS was initial and final concentration of WMO by Soxhlet, at the PHYTO, phenology and biomass of C. arietinum were taken at seedling. The experimental data were analyzed by ANOVA/Tukey HSD P <0.05%. Results showed that BIS and PHYTO of soil impacted by 90000 ppm of WMO decreased until 1200 ppm numerical value statistically different compared to 79000 ppm of soil polluted by WMO, without BIS and either PHYTO or negative control. Those results showed that BIS and PHYTO are an effective technique for recovering soil polluting by relative high level of WMO according to NOM-138.","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43065205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective was to evaluate the in vitro antifungal activity of the diluted crude extract of the scorpion venom Hadruroides charcasus (Karsch 1879) against Candida albicans. It was an experimental study, 54 experimental units were used (9 poison concentrations, 2 strains and three repetitions). The venom of the scorpion was obtained by electrical stimulation. The double serial dilution method was determined at different concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, 0.39%. To evaluate the antifungal activity, the microdilution method was used to determine the Minimum Inhibitory Concentration (MIC). The results showed that by means of the microdilution method that for the strain n ° 1 presented a minimum inhibitory concentration (MIC) to 25% of the diluted raw venom, very different was for the strain n ° 2 did not present CMI. It was concluded that the findings of the current study show that the diluted crude extract of the scorpion venom H. charcasus possesses antifungal activity on strains of C. albicans, being possible to use it as a template for the design and development of new antifungal drugs.
{"title":"Actividad antifúngica in vitro del extracto crudo diluido del veneno de escorpión Hadruroides charcasus (Karsch 1879) frente a Candida albicans","authors":"Nileth Daysy Rivera-Coronado, Orlando Pérez-Delgado","doi":"10.36610/J.JSARS.2019.100200096","DOIUrl":"https://doi.org/10.36610/J.JSARS.2019.100200096","url":null,"abstract":"The objective was to evaluate the in vitro antifungal activity of the diluted crude extract of the scorpion venom Hadruroides charcasus (Karsch 1879) against Candida albicans. It was an experimental study, 54 experimental units were used (9 poison concentrations, 2 strains and three repetitions). The venom of the scorpion was obtained by electrical stimulation. The double serial dilution method was determined at different concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, 0.39%. To evaluate the antifungal activity, the microdilution method was used to determine the Minimum Inhibitory Concentration (MIC). The results showed that by means of the microdilution method that for the strain n ° 1 presented a minimum inhibitory concentration (MIC) to 25% of the diluted raw venom, very different was for the strain n ° 2 did not present CMI. It was concluded that the findings of the current study show that the diluted crude extract of the scorpion venom H. charcasus possesses antifungal activity on strains of C. albicans, being possible to use it as a template for the design and development of new antifungal drugs.","PeriodicalId":53763,"journal":{"name":"Journal of the Selva Andina Research Society","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43105757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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