Pub Date : 2024-06-26DOI: 10.1021/acs.nanolett.4c01757
Kai Yang, Daotong Zhang, Zhao Li, Tao Zhang, Chaozheng Liu, Pei Yang, Guoqiang Zhou, Min Luo, Yiying Ling, Weimin Chen, Xiaoyan Zhou
The shrinkage and collapse of wood cell walls during carbonization make it challenging to control the size and shape of carbonized wood (CW) through pre- or postprocessing (e.g., sawing, cutting, and milling). Herein, a shape-adaptive MXene shell (MS) is created on the surface of the wood cell walls. The MS limits the deformation of wood cell walls by spatial confinement and traction effects, which is supported by the inherent dimensional stability of the MS and the formation of new C-O-Ti covalent bonds between the wood cell wall and MS. Consequently, the volumetric shrinkage ratio of CW encapsulated by the MS (CW-MS) is significantly reduced from 54.8% for CW to 2.6% for CW-MS even at 800 °C. The harnessing of this collapse enables the production of CW-MS with prolonged stability and high electric conductivity (384 S m-1). These properties make CW-MS suitable for energy storage devices with various designed shapes, matching the increasingly compact and complex structures of electronic devices.
{"title":"Size/Shape-Controllable Carbonized Wood Electrodes Enabled by an MXene Shell with Spatial Confinement and a Traction Effect on the Wood Cell Wall for Shape-Customizable Energy Storage Devices.","authors":"Kai Yang, Daotong Zhang, Zhao Li, Tao Zhang, Chaozheng Liu, Pei Yang, Guoqiang Zhou, Min Luo, Yiying Ling, Weimin Chen, Xiaoyan Zhou","doi":"10.1021/acs.nanolett.4c01757","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c01757","url":null,"abstract":"<p><p>The shrinkage and collapse of wood cell walls during carbonization make it challenging to control the size and shape of carbonized wood (CW) through pre- or postprocessing (e.g., sawing, cutting, and milling). Herein, a shape-adaptive MXene shell (MS) is created on the surface of the wood cell walls. The MS limits the deformation of wood cell walls by spatial confinement and traction effects, which is supported by the inherent dimensional stability of the MS and the formation of new C-O-Ti covalent bonds between the wood cell wall and MS. Consequently, the volumetric shrinkage ratio of CW encapsulated by the MS (CW-MS) is significantly reduced from 54.8% for CW to 2.6% for CW-MS even at 800 °C. The harnessing of this collapse enables the production of CW-MS with prolonged stability and high electric conductivity (384 S m<sup>-1</sup>). These properties make CW-MS suitable for energy storage devices with various designed shapes, matching the increasingly compact and complex structures of electronic devices.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141453744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-26DOI: 10.1021/acs.nanolett.4c00418
Antonio Fieramosca, Rosanna Mastria, Kevin Dini, Lorenzo Dominici, Laura Polimeno, Marco Pugliese, Carmela Tania Prontera, Luisa De Marco, Vincenzo Maiorano, Francesco Todisco, Dario Ballarini, Milena De Giorgi, Giuseppe Gigli, Timothy C H Liew, Daniele Sanvitto
The realization of efficient optical devices depends on the ability to harness strong nonlinearities, which are challenging to achieve with standard photonic systems. Exciton-polaritons formed in hybrid organic-inorganic perovskites offer a promising alternative, exhibiting strong interactions at room temperature (RT). Despite recent demonstrations showcasing a robust nonlinear response, further progress is hindered by an incomplete understanding of the microscopic mechanisms governing polariton interactions in perovskite-based strongly coupled systems. Here, we investigate the nonlinear properties of quasi-2D dodecylammonium lead iodide perovskite (n3-C12) crystals embedded in a planar microcavity. Polarization-resolved pump-probe measurements reveal the contribution of indirect exchange interactions assisted by dark states formation. Additionally, we identify a strong dependence of the unique spin-dependent interaction of polaritons on sample detuning. The results are pivotal for the advancement of polaritonics, and the tunability of the robust spin-dependent anisotropic interaction in n3-C12 perovskites makes this material a powerful choice for the realization of polaritonic circuits.
{"title":"Origin of Exciton-Polariton Interactions and Decoupled Dark States Dynamics in 2D Hybrid Perovskite Quantum Wells.","authors":"Antonio Fieramosca, Rosanna Mastria, Kevin Dini, Lorenzo Dominici, Laura Polimeno, Marco Pugliese, Carmela Tania Prontera, Luisa De Marco, Vincenzo Maiorano, Francesco Todisco, Dario Ballarini, Milena De Giorgi, Giuseppe Gigli, Timothy C H Liew, Daniele Sanvitto","doi":"10.1021/acs.nanolett.4c00418","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c00418","url":null,"abstract":"<p><p>The realization of efficient optical devices depends on the ability to harness strong nonlinearities, which are challenging to achieve with standard photonic systems. Exciton-polaritons formed in hybrid organic-inorganic perovskites offer a promising alternative, exhibiting strong interactions at room temperature (RT). Despite recent demonstrations showcasing a robust nonlinear response, further progress is hindered by an incomplete understanding of the microscopic mechanisms governing polariton interactions in perovskite-based strongly coupled systems. Here, we investigate the nonlinear properties of quasi-2D dodecylammonium lead iodide perovskite (n3-C12) crystals embedded in a planar microcavity. Polarization-resolved pump-probe measurements reveal the contribution of indirect exchange interactions assisted by dark states formation. Additionally, we identify a strong dependence of the unique spin-dependent interaction of polaritons on sample detuning. The results are pivotal for the advancement of polaritonics, and the tunability of the robust spin-dependent anisotropic interaction in n3-C12 perovskites makes this material a powerful choice for the realization of polaritonic circuits.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141453743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-26DOI: 10.1021/acs.nanolett.4c02233
Jin Ho Lee, Wonsik Jang, Hojeong Lee, Daewon Oh, Woo Yeong Noh, Kwang Young Kim, Jongkyoung Kim, Hyoseok Kim, Kwangjin An, Min Gyu Kim, Youngkook Kwon, Jae Sung Lee, Seungho Cho
Electrochemical CO2 reduction reaction (eCO2RR) over Cu-based catalysts is a promising approach for efficiently converting CO2 into value-added chemicals and alternative fuels. However, achieving controllable product selectivity from eCO2RR remains challenging because of the difficulty in controlling the oxidation states of Cu against robust structural reconstructions during the eCO2RR. Herein, we report a novel strategy for tuning the oxidation states of Cu species and achieving eCO2RR product selectivity by adjusting the Cu content in CuMgAl-layered double hydroxide (LDH)-based catalysts. In this strategy, the highly stable Cu2+ species in low-Cu-containing LDHs facilitated the strong adsorption of *CO intermediates and further hydrogenation into CH4. Conversely, the mixed Cu0/Cu+ species in high-Cu-containing LDHs derived from the electroreduction during the eCO2RR accelerated C-C coupling reactions. This strategy to regulate Cu oxidation states using LDH nanostructures with low and high Cu molar ratios produced an excellent eCO2RR performance for CH4 and C2+ products, respectively.
{"title":"Tuning CuMgAl-Layered Double Hydroxide Nanostructures to Achieve CH<sub>4</sub> and C<sub>2+</sub> Product Selectivity in CO<sub>2</sub> Electroreduction.","authors":"Jin Ho Lee, Wonsik Jang, Hojeong Lee, Daewon Oh, Woo Yeong Noh, Kwang Young Kim, Jongkyoung Kim, Hyoseok Kim, Kwangjin An, Min Gyu Kim, Youngkook Kwon, Jae Sung Lee, Seungho Cho","doi":"10.1021/acs.nanolett.4c02233","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c02233","url":null,"abstract":"<p><p>Electrochemical CO<sub>2</sub> reduction reaction (eCO<sub>2</sub>RR) over Cu-based catalysts is a promising approach for efficiently converting CO<sub>2</sub> into value-added chemicals and alternative fuels. However, achieving controllable product selectivity from eCO<sub>2</sub>RR remains challenging because of the difficulty in controlling the oxidation states of Cu against robust structural reconstructions during the eCO<sub>2</sub>RR. Herein, we report a novel strategy for tuning the oxidation states of Cu species and achieving eCO<sub>2</sub>RR product selectivity by adjusting the Cu content in CuMgAl-layered double hydroxide (LDH)-based catalysts. In this strategy, the highly stable Cu<sup>2+</sup> species in low-Cu-containing LDHs facilitated the strong adsorption of *CO intermediates and further hydrogenation into CH<sub>4</sub>. Conversely, the mixed Cu<sup>0</sup>/Cu<sup>+</sup> species in high-Cu-containing LDHs derived from the electroreduction during the eCO<sub>2</sub>RR accelerated C-C coupling reactions. This strategy to regulate Cu oxidation states using LDH nanostructures with low and high Cu molar ratios produced an excellent eCO<sub>2</sub>RR performance for CH<sub>4</sub> and C<sub>2+</sub> products, respectively.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141453747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Osseointegration is the most important factor determining implant success. The surface modification of TiO2 nanotubes prepared by anodic oxidation has remarkable advantages in promoting bone formation. However, the mechanism behind this phenomenon is still unintelligible. Here we show that the nanomorphology exhibited open and clean nanotube structure and strong hydrophilicity, and the nanomorphology significantly facilitated the adhesion, proliferation, and osteogenesis differentiation of stem cells. Exploring the mechanism, we found that the nanomorphology can enhance mitochondrial oxidative phosphorylation (OxPhos) by activating Piezo1 and increasing intracellular Ca2+. The increase in OxPhos can significantly uplift the level of acetyl-CoA in the cytoplasm but not significantly raise the level of acetyl-CoA in the nucleus, which was beneficial for the acetylation and stability of β-catenin and ultimately promoted osteogenesis. This study provides a new interpretation for the regulatory mechanism of stem cell osteogenesis by nanomorphology.
{"title":"Nanoporous Titanium Implant Surface Accelerates Osteogenesis via the Piezo1/Acetyl-CoA/β-Catenin Pathway.","authors":"Qian Zhang, Run-Long Pan, Hui Wang, Jun-Jun Wang, Song-He Lu, Min Zhang","doi":"10.1021/acs.nanolett.4c01101","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c01101","url":null,"abstract":"<p><p>Osseointegration is the most important factor determining implant success. The surface modification of TiO<sub>2</sub> nanotubes prepared by anodic oxidation has remarkable advantages in promoting bone formation. However, the mechanism behind this phenomenon is still unintelligible. Here we show that the nanomorphology exhibited open and clean nanotube structure and strong hydrophilicity, and the nanomorphology significantly facilitated the adhesion, proliferation, and osteogenesis differentiation of stem cells. Exploring the mechanism, we found that the nanomorphology can enhance mitochondrial oxidative phosphorylation (OxPhos) by activating Piezo1 and increasing intracellular Ca<sup>2+</sup>. The increase in OxPhos can significantly uplift the level of acetyl-CoA in the cytoplasm but not significantly raise the level of acetyl-CoA in the nucleus, which was beneficial for the acetylation and stability of β-catenin and ultimately promoted osteogenesis. This study provides a new interpretation for the regulatory mechanism of stem cell osteogenesis by nanomorphology.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Metal selenides show outstanding sodium-ion storage performance when matched with an ether-based electrolyte. However, the intrinsic origin of improvement and deterministic interface characteristics have not been systematically elucidated. Herein, employing FeSe2 anode as the model system, the electrochemical kinetics of metal selenides in ether and ester-based electrolytes and associated solid electrolyte interphase (SEI) are investigated in detail. Based on the galvanostatic intermittent titration technique and in situ electrochemical impedance spectroscopy, it is found that the ether-based electrolyte can ensure fast Na+ transfer and low interface impedance. Additionally, the ether-derived thin and smooth double-layer SEI, which is critical in facilitating ion transport, maintaining structural stability, and inhibiting electrolyte overdecomposition, is concretely visualized by transmission electron microscopy, atomic force microscopy, and depth-profiling X-ray photoelectron spectroscopy. This work provides a deep understanding of the optimization mechanism of electrolytes, which can guide available inspiration for the design of practical electrode materials.
当金属硒化物与醚基电解质配合使用时,会显示出出色的钠离子存储性能。然而,其改善的内在原因和确定性界面特征尚未得到系统阐明。本文以 FeSe2 阳极为模型系统,详细研究了金属硒化物在醚基和酯基电解质中的电化学动力学以及相关的固态电解质间相(SEI)。基于电静电间歇滴定技术和原位电化学阻抗谱,研究发现醚基电解质能确保快速的 Na+ 转移和较低的界面阻抗。此外,通过透射电子显微镜、原子力显微镜和深度剖析 X 射线光电子能谱,可以具体观察到由醚衍生的薄而光滑的双层 SEI,这对于促进离子传输、保持结构稳定和抑制电解质过度分解至关重要。这项研究深入理解了电解质的优化机理,对设计实用电极材料具有指导意义。
{"title":"Unraveling the Intrinsic Origin of the Superior Sodium-Ion Storage Performance of Metal Selenides Anode in Ether-Based Electrolytes.","authors":"Yuteng Gong, Yu Li, Ying Li, Mingquan Liu, Xin Feng, Yufeng Sun, Feng Wu, Chuan Wu, Ying Bai","doi":"10.1021/acs.nanolett.4c02145","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c02145","url":null,"abstract":"<p><p>Metal selenides show outstanding sodium-ion storage performance when matched with an ether-based electrolyte. However, the intrinsic origin of improvement and deterministic interface characteristics have not been systematically elucidated. Herein, employing FeSe<sub>2</sub> anode as the model system, the electrochemical kinetics of metal selenides in ether and ester-based electrolytes and associated solid electrolyte interphase (SEI) are investigated in detail. Based on the galvanostatic intermittent titration technique and in situ electrochemical impedance spectroscopy, it is found that the ether-based electrolyte can ensure fast Na<sup>+</sup> transfer and low interface impedance. Additionally, the ether-derived thin and smooth double-layer SEI, which is critical in facilitating ion transport, maintaining structural stability, and inhibiting electrolyte overdecomposition, is concretely visualized by transmission electron microscopy, atomic force microscopy, and depth-profiling X-ray photoelectron spectroscopy. This work provides a deep understanding of the optimization mechanism of electrolytes, which can guide available inspiration for the design of practical electrode materials.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two-dimensional (2D) lead halide perovskites are excellent candidates for X-ray detection due to their high resistivity, high ion migration barrier, and large X-ray absorption coefficients. However, the high toxicity and long interlamellar distance of the 2D perovskites limit their wide application in high sensitivity X-ray detection. Herein, we demonstrate stable and toxicity-reduced 2D perovskite single crystals (SCs) realized by interlamellar-spacing engineering via a distortion self-balancing strategy. The engineered low-toxicity 2D SC detectors achieve high stability, large mobility-lifetime product, and therefore high-performance X-ray detection. Specifically, the detectors exhibit a record high sensitivity of 13488 μC Gy1- cm-2, a low detection limit of 8.23 nGy s-1, as well as a high spatial resolution of 8.56 lp mm-1 in X-ray imaging, all of which are far better than those of the high-toxicity 2D lead-based perovskite detectors. These advances provide a new technical solution for the low-cost fabrication of low-toxicity, scalable X-ray detectors.
二维(2D)卤化铅包晶石具有高电阻率、高离子迁移障碍和大 X 射线吸收系数,是 X 射线探测的绝佳候选材料。然而,二维过氧化物的高毒性和较长的层间距离限制了它们在高灵敏度 X 射线探测中的广泛应用。在此,我们展示了通过畸变自平衡策略进行层间距工程而实现的稳定且毒性降低的二维包光体单晶体(SC)。这种工程化的低毒二维SC探测器具有高稳定性、大迁移率-寿命乘积,因此可实现高性能的X射线探测。具体来说,该探测器的灵敏度高达 13488 μC Gy1- cm-2,探测极限低至 8.23 nGy s-1,在 X 射线成像中的空间分辨率高达 8.56 lp mm-1,均远远优于高毒性二维铅基包晶探测器。这些进步为低成本制造低毒性、可扩展的 X 射线探测器提供了新的技术解决方案。
{"title":"Interlamellar-Spacing Engineering of Stable and Toxicity-Reduced 2D Perovskite Single Crystal for High-Resolution X-ray Imaging.","authors":"Yuqian Liang, Zeqin Zhao, Jinglu Hao, Yunxia Zhang, Depeng Chu, Binxia Jia, Jiacheng Pi, Lei Zhao, Mingyue Wei, Ziyang Feng, Yaohui Li, Ruixin Shi, Xiaojie Zhang, Zupei Yang, Xiaolian Chao, Shengzhong Frank Liu, Yucheng Liu","doi":"10.1021/acs.nanolett.4c02507","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c02507","url":null,"abstract":"<p><p>Two-dimensional (2D) lead halide perovskites are excellent candidates for X-ray detection due to their high resistivity, high ion migration barrier, and large X-ray absorption coefficients. However, the high toxicity and long interlamellar distance of the 2D perovskites limit their wide application in high sensitivity X-ray detection. Herein, we demonstrate stable and toxicity-reduced 2D perovskite single crystals (SCs) realized by interlamellar-spacing engineering via a distortion self-balancing strategy. The engineered low-toxicity 2D SC detectors achieve high stability, large mobility-lifetime product, and therefore high-performance X-ray detection. Specifically, the detectors exhibit a record high sensitivity of 13488 μC Gy<sup>1-</sup> cm<sup>-2</sup>, a low detection limit of 8.23 nGy s<sup>-1</sup>, as well as a high spatial resolution of 8.56 lp mm<sup>-1</sup> in X-ray imaging, all of which are far better than those of the high-toxicity 2D lead-based perovskite detectors. These advances provide a new technical solution for the low-cost fabrication of low-toxicity, scalable X-ray detectors.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enhancing the antitumor immune response and targeting ability of oncolytic viruses will improve the effect of tumor immunotherapy. Through infecting neural stem cells (NSCs) with a capsid dual-modified oncolytic adenovirus (CRAd), we obtained and characterized the "oncolytic extracellular vesicles" (CRAdEV) with improved targeted infection and tumor killing activity compared with CRAd. Both ex vivo and in vivo studies revealed that CRAdEV activated innate immune cells and importantly enhanced the immunomodulatory effect compared to CRAd. We found that CRAdEV effectively increased the number of DCs and activated CD4+ and CD8+ T cells, significantly increased the number and activation of B cells, and produced higher levels of tumor-specific antibodies, thus eliciting enhanced antitumor activity compared with CRAd in a B16 xenograft immunocompetent mice model. This study provides a novel approach to oncolytic adenovirus modification and demonstrates the potential of "oncolytic extracellular vesicles" in antitumor immunotherapy.
{"title":"Antitumor Effect and Immunomodulatory Mechanism of \"Oncolytic Extracellular Vesicles\".","authors":"Xinyao Feng, Wenmo Liu, Xinyuan Jia, Fangshen Li, Xupu Wang, Xinyao Liu, Jiahao Yu, Xiaolei Lin, Haihong Zhang, Chu Wang, Hui Wu, Jiaxin Wu, Bin Yu, Xianghui Yu","doi":"10.1021/acs.nanolett.4c02279","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c02279","url":null,"abstract":"<p><p>Enhancing the antitumor immune response and targeting ability of oncolytic viruses will improve the effect of tumor immunotherapy. Through infecting neural stem cells (NSCs) with a capsid dual-modified oncolytic adenovirus (CRAd), we obtained and characterized the \"oncolytic extracellular vesicles\" (CRAdEV) with improved targeted infection and tumor killing activity compared with CRAd. Both <i>ex vivo</i> and <i>in vivo</i> studies revealed that CRAdEV activated innate immune cells and importantly enhanced the immunomodulatory effect compared to CRAd. We found that CRAdEV effectively increased the number of DCs and activated CD4<sup>+</sup> and CD8<sup>+</sup> T cells, significantly increased the number and activation of B cells, and produced higher levels of tumor-specific antibodies, thus eliciting enhanced antitumor activity compared with CRAd in a B16 xenograft immunocompetent mice model. This study provides a novel approach to oncolytic adenovirus modification and demonstrates the potential of \"oncolytic extracellular vesicles\" in antitumor immunotherapy.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanodevices that function in specific organs or cells are one of the ultimate goals of synthetic biology. The recent progress in DNA nanotechnology such as DNA origami has allowed us to construct nanodevices to deliver a payload (e.g., drug) to the tumor. However, delivery to specific organs remains difficult due to the fragility of the DNA nanostructure and the low targeting capability of the DNA nanostructure. Here, we constructed tough DNA origami that allowed us to encapsulate the DNA origami into lipid-based nanoparticles (LNPs) under harsh conditions (low pH), harnessing organ-specific delivery of the gene of interest (GOI). We found that DNA origami-encapsulated LNPs can increase the functionality of payload GOIs (mRNA and siRNA) inside mouse organs through the contribution from different LNP structures revealed by cryogenic electron microscope (Cryo-EM). These data should be the basis for future organ-specific gene expression control using DNA origami nanodevices.
在特定器官或细胞中发挥作用的纳米装置是合成生物学的终极目标之一。DNA 纳米技术(如 DNA 折纸)的最新进展使我们能够构建纳米装置,将有效载荷(如药物)输送到肿瘤。然而,由于 DNA 纳米结构的脆弱性和较低的靶向能力,向特定器官递送仍然很困难。在这里,我们构建了坚韧的DNA折纸,使我们能够在苛刻的条件(低pH值)下将DNA折纸封装到脂基纳米颗粒(LNPs)中,利用器官特异性递送感兴趣的基因(GOI)。我们发现,通过低温电子显微镜(Cryo-EM)显示的不同 LNP 结构的贡献,DNA 折纸封装的 LNPs 可以提高有效载荷 GOIs(mRNA 和 siRNA)在小鼠器官内的功能。这些数据应成为未来利用DNA折纸纳米器件控制器官特异性基因表达的基础。
{"title":"Organ-Specific Gene Expression Control Using DNA Origami-Based Nanodevices.","authors":"Yuxiang Liu, Ruixuan Wang, Qimingxing Chen, Yan Chang, Qi Chen, Kodai Fukumoto, Bingxun Wang, Jianchen Yu, Changfeng Luo, Jiayuan Ma, Xiaoxia Chen, Yuko Murayama, Kenichi Umeda, Noriyuki Kodera, Yoshie Harada, Shun-Ichi Sekine, Jianfeng Li, Hisashi Tadakuma","doi":"10.1021/acs.nanolett.4c02104","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c02104","url":null,"abstract":"<p><p>Nanodevices that function in specific organs or cells are one of the ultimate goals of synthetic biology. The recent progress in DNA nanotechnology such as DNA origami has allowed us to construct nanodevices to deliver a payload (e.g., drug) to the tumor. However, delivery to specific organs remains difficult due to the fragility of the DNA nanostructure and the low targeting capability of the DNA nanostructure. Here, we constructed tough DNA origami that allowed us to encapsulate the DNA origami into lipid-based nanoparticles (LNPs) under harsh conditions (low pH), harnessing organ-specific delivery of the gene of interest (GOI). We found that DNA origami-encapsulated LNPs can increase the functionality of payload GOIs (mRNA and siRNA) inside mouse organs through the contribution from different LNP structures revealed by cryogenic electron microscope (Cryo-EM). These data should be the basis for future organ-specific gene expression control using DNA origami nanodevices.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-25DOI: 10.1021/acs.nanolett.4c00939
Sang Bin Jeong, Ki Joon Heo, Jae Hyun Yoo, Dong-Gi Kang, Leonardo Santoni, Caroline E. Knapp, Andreas Kafizas, Claire J. Carmalt, Ivan P. Parkin, Jae Hak Shin, Gi Byoung Hwang, Jae Hee Jung
Herein, we introduce a photobiocidal surface activated by white light. The photobiocidal surface was produced through thermocompressing a mixture of titanium dioxide (TiO2), ultra-high-molecular-weight polyethylene (UHMWPE), and reduced graphene oxide (rGO) powders. A photobiocidal activity was not observed on UHMWPE-TiO2. However, UHMWPE-TiO2@rGO exhibited potent photobiocidal activity (>3-log reduction) against Staphylococcus epidermidis and Escherichia coli bacteria after a 12 h exposure to white light. The activity was even more potent against the phage phi 6 virus, a SARS-CoV-2 surrogate, with a >5-log reduction after 6 h exposure to white light. Our mechanistic studies showed that the UHMWPE-TiO2@rGO was activated only by UV light, which accounts for 0.31% of the light emitted by the white LED lamp, producing reactive oxygen species that are lethal to microbes. This indicates that adding rGO to UHMWPE-TiO2 triggered intense photobiocidal activity even at shallow UV flux levels.
{"title":"Photobiocidal Activity of TiO2/UHMWPE Composite Activated by Reduced Graphene Oxide under White Light","authors":"Sang Bin Jeong, Ki Joon Heo, Jae Hyun Yoo, Dong-Gi Kang, Leonardo Santoni, Caroline E. Knapp, Andreas Kafizas, Claire J. Carmalt, Ivan P. Parkin, Jae Hak Shin, Gi Byoung Hwang, Jae Hee Jung","doi":"10.1021/acs.nanolett.4c00939","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c00939","url":null,"abstract":"Herein, we introduce a photobiocidal surface activated by white light. The photobiocidal surface was produced through thermocompressing a mixture of titanium dioxide (TiO<sub>2</sub>), ultra-high-molecular-weight polyethylene (UHMWPE), and reduced graphene oxide (rGO) powders. A photobiocidal activity was not observed on UHMWPE-TiO<sub>2</sub>. However, UHMWPE-TiO<sub>2</sub>@rGO exhibited potent photobiocidal activity (>3-log reduction) against <i>Staphylococcus epidermidis</i> and <i>Escherichia coli</i> bacteria after a 12 h exposure to white light. The activity was even more potent against the phage phi 6 virus, a SARS-CoV-2 surrogate, with a >5-log reduction after 6 h exposure to white light. Our mechanistic studies showed that the UHMWPE-TiO<sub>2</sub>@rGO was activated only by UV light, which accounts for 0.31% of the light emitted by the white LED lamp, producing reactive oxygen species that are lethal to microbes. This indicates that adding rGO to UHMWPE-TiO<sub>2</sub> triggered intense photobiocidal activity even at shallow UV flux levels.","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":10.8,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141448766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mechanical stress significantly affects the physiological functions of cells, including tissue homeostasis, cytoskeletal alterations, and intracellular transport. As a major cytoskeletal component, microtubules respond to mechanical stimulation by altering their alignment and polymerization dynamics. Previously, we reported that microtubules may modulate cargo transport by one of the microtubule-associated motor proteins, dynein, under compressive mechanical stress. Despite the critical role of tensile stress in many biological functions, how tensile stress on microtubules regulates cargo transport is yet to be unveiled. The present study demonstrates that the low-level tensile stress-induced microtubule deformation facilitates dynein-driven transport. We validate our experimental findings using all-atom molecular dynamics simulation. Our study may provide important implications for developing new therapies for diseases that involve impaired intracellular transport.
{"title":"Tensile Stress on Microtubules Facilitates Dynein-Driven Cargo Transport.","authors":"Syeda Rubaiya Nasrin, Takefumi Yamashita, Mitsunori Ikeguchi, Takayuki Torisawa, Kazuhiro Oiwa, Kazuki Sada, Akira Kakugo","doi":"10.1021/acs.nanolett.4c00209","DOIUrl":"https://doi.org/10.1021/acs.nanolett.4c00209","url":null,"abstract":"<p><p>Mechanical stress significantly affects the physiological functions of cells, including tissue homeostasis, cytoskeletal alterations, and intracellular transport. As a major cytoskeletal component, microtubules respond to mechanical stimulation by altering their alignment and polymerization dynamics. Previously, we reported that microtubules may modulate cargo transport by one of the microtubule-associated motor proteins, dynein, under compressive mechanical stress. Despite the critical role of tensile stress in many biological functions, how tensile stress on microtubules regulates cargo transport is yet to be unveiled. The present study demonstrates that the low-level tensile stress-induced microtubule deformation facilitates dynein-driven transport. We validate our experimental findings using all-atom molecular dynamics simulation. Our study may provide important implications for developing new therapies for diseases that involve impaired intracellular transport.</p>","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":null,"pages":null},"PeriodicalIF":9.6,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}