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Preliminary results on tartaric stabilization of red wine by adding different carboxymethylcelluloses 添加不同羧甲基纤维素对红酒酒石稳定性的初步研究
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183301047
E. Pittari, S. Catarino, M. C. Andrade, J. Ricardo-da-Silva
Despite being natural phenomena of physical-chemical stabilization of young wines, tartrate precipitations in bottled wines are often understood as a quality fault. Carboxymethylcellulose (CMC) is an inhibitor of tartaric salts crystallization, authorized as oenological additive, which application is currently limited to white and sparkling wines by OIV. The aim of this exploratory research was to study the effect of different CMC on a (tartaric) unstable red wine, in terms of tartrate stability, colouring matter stability, turbidity, comprehensive phenolic composition, and chromatic characteristics. All the CMC tested were able to inhibit potassium bitartrate crystallization in the red wine, promoting its tartaric stability. Slight increase in the colour intensity, coloured anthocyanins concentration, and turbidity of the wine were observed. No colouring matter precipitation was verified. The study seems to indicate that the CMC can be an efficient alternative to other tartaric stabilization treatments, also in red wines, opening new prospective and scenarios concerning the use of CMC in this type of wines.
尽管酒石酸盐沉淀是年轻葡萄酒的物理化学稳定的自然现象,但瓶装葡萄酒中的酒石酸盐沉淀通常被理解为质量缺陷。羧甲基纤维素(CMC)是一种酒石酸盐结晶抑制剂,被OIV批准为酿酒添加剂,目前仅用于白葡萄酒和起泡葡萄酒。本探索性研究的目的是研究不同CMC对酒石酸盐稳定性、着色剂稳定性、浊度、综合酚类成分和色度特性对酒石酸不稳定红葡萄酒的影响。所测CMC均能抑制酒石酸钾在红酒中的结晶,提高其酒石酸稳定性。观察到葡萄酒的颜色强度、有色花青素浓度和浊度略有增加。证实无着色剂沉淀。该研究似乎表明,CMC可以成为其他酒石质稳定处理的有效替代方法,也适用于红葡萄酒,为CMC在这类葡萄酒中的应用开辟了新的前景和场景。
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引用次数: 4
Alterations on phenolic compounds and antioxidant activity during sour grape juice concentrate processing 酸葡萄汁浓缩过程中酚类化合物及抗氧化活性的变化
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183302136
A. Guler, Ö. Tokuşoğlu, N. Artık
The alterations of phenolic compounds and antioxidant capacity of sour grape juice were investigated during the concentration process stages. Phenolics and antioxidant properties of the samples changed more at the vacuum evaporation stage than that of the other stages. After evaporation, the antioxidant capacity of the samples decreased approximately between 14.2 and 17.0 % for DPPH and ABTS methods, respectively. Besides, phenolic contents also decreased approximately as 14.8%. HPLC data on phenolics of sour grape juice during concentrate processing gave 12 polyphenols, including gallic acid, (+)-catechin, (-)-epigallocatechin, vanillic acid, (-)-epigallocatechin gallate, (-)-epicatechin, caftaric acid, caffeic acid and p -coumaric acid, which were determined as 1.05-1.83 mg/100 g, 5.40-7.83 mg/100 g, 6.35-9.21 mg/100 g, 3.01-5.18 mg/100 g, 0- 1.95 mg/100 g, 2.33-3.54 mg/100 g, 12.40-37.60 mg/100 g, 1.44-2.26 mg/100 g and 0.27-0.44 mg/100 g, respectively. Ferulic, sinapic acids and quercetin were only detected at concentrate step of the sour grape juice processing and were found as 0.18 mg/100 g, 0.28 mg/100 g and 0.76 mg/100 g, respectively.
研究了酸葡萄汁在浓缩过程中酚类化合物和抗氧化能力的变化。样品的酚类物质和抗氧化性能在真空蒸发阶段的变化大于其他阶段。蒸发后,DPPH法和ABTS法样品的抗氧化能力分别下降约14.2% ~ 17.0%。酚类物质含量也下降了约14.8%。酸葡萄汁浓缩液中酚类物质的HPLC测定结果为:没食子酸、(+)-儿茶素、(-)-表没食子儿茶素、香草酸、(-)-表没食子儿茶素没食子酸酯、(-)-表儿茶素、果酸、咖啡酸、对香豆酸等12种多酚,测定值分别为1.05 ~ 1.83 mg/100 g、5.40 ~ 7.83 mg/100 g、6.35 ~ 9.21 mg/100 g、3.01 ~ 5.18 mg/100 g、0 ~ 1.95 mg/100 g、2.33 ~ 3.54 mg/100 g、12.40 ~ 37.60 mg/100 g、1.44 ~ 2.26 mg/100 g和0.27 ~ 0.44 mg/100 g。阿魏酸、辛酸和槲皮素仅在酸葡萄汁加工浓缩阶段检测到,含量分别为0.18 mg/100 g、0.28 mg/100 g和0.76 mg/100 g。
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引用次数: 6
Physiological and agronomical responses to environmental fluctuations of two Portuguese grapevine varieties during three field seasons 两个葡萄牙葡萄品种在三个田间季节对环境波动的生理和农艺反应
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183301001
Luísa C. Carvalho, J. Coito, Elsa Gonçalves, Carlos Lopes, S. Amâncio
Extensive agricultural losses are attributed to heat, often combined with drought. These abiotic stresses occur in the field simultaneously, namely in areas with Mediterranean climate, where grapevine traditionally grows. The available scenarios for climate change suggest an increase in the frequency of heat waves and severe drought events in summer, also affecting the South of Portugal. In this work we monitored several production-related parameters and evaluated the state of the oxidative stress response apparatus of two grapevine varieties, Touriga Nacional (TN) and Trincadeira (TR), with and without irrigation, during three field seasons (2010 to 2012). Overall, results point to a high correlation of most yield and stress-associated parameters with the specific characteristics of each variety and to each season rather than the irrigation treatments. In the season with the driest winter, 2012, the lack of irrigation significantly affected yield in TR, while in the two other seasons the impact of the irrigation regime was much lower. In 2012, the yield of TN was affected by environmental conditions of the previous season. The irrigation treatments significantly affected berry size rather than quality.
广泛的农业损失归因于高温,通常与干旱相结合。这些非生物胁迫同时发生在田间,即在葡萄传统生长的地中海气候地区。现有的气候变化情景表明,夏季热浪和严重干旱事件的频率会增加,也会影响到葡萄牙南部。在本研究中,我们监测了两个葡萄品种Touriga Nacional (TN)和Trincadeira (TR)的几个生产相关参数,并评估了两个葡萄品种在灌溉和不灌溉的情况下氧化应激反应装置的状态(2010 - 2012)。总体而言,结果表明,大多数产量和与胁迫相关的参数与每个品种和每个季节的具体特征高度相关,而不是灌溉处理。在2012年冬季最干旱的季节,缺乏灌溉对TR产量的影响显著,而在其他两个季节,灌溉制度的影响要小得多。2012年全氮产量受前一季环境条件的影响。灌水处理显著影响果实大小而非品质。
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引用次数: 3
Portuguese vines and wines: heritage, quality symbol, tourism asset. 葡萄牙葡萄和葡萄酒:遗产,品质象征,旅游资产。
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183301031
Lavrador da Silva, M. J. Fernão-Pires, F. Bianchi-de-Aguiar
This paper aims to showcase the value and uniqueness of Portuguese wines and wine regions. It has three independent but related parts. The first part focuses the millenary history of vines in Portugal and stresses their value for the regional and national economy. The second part focuses on the role of brands in the wine regions and wine marketing, highlighting the importance of the landscape in promotion. It is claimed that the use of landmarks can help create an identity image, useful for attracting tourism and wine advertising, being also a major asset for the Portuguese world trade of wines. Landmarks identification and promotional suggestions with landmarks related to Portuguese wine regions are presented. The third part presents a short revision of the panorama of wine tourism in Portugal and stresses the role of landscapes in its promotion. A literary route in the Douro Demarcated Region is presented by way of example.
本文旨在展示葡萄牙葡萄酒和葡萄酒产区的价值和独特性。它有三个独立但相关的部分。第一部分重点介绍了葡萄牙葡萄树的千年历史,并强调了它们对地区和国家经济的价值。第二部分侧重于品牌在葡萄酒产区和葡萄酒营销中的作用,突出了景观在推广中的重要性。据称,使用地标可以帮助创造一个身份形象,有助于吸引游客和葡萄酒广告,也是葡萄牙世界葡萄酒贸易的主要资产。提出了与葡萄牙葡萄酒产区相关的地标识别和推广建议。第三部分介绍了葡萄牙葡萄酒旅游全景的简短修订,并强调了景观在其推广中的作用。通过实例介绍了斗罗界域的文学路线。
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引用次数: 16
Differential genetic stability in vineyards of the cultivar ‘Italy’ (Vitis vinifera L.) cultivated in different regions of Southern and Southwestern Brazil 栽培于巴西南部和西南部不同地区的“意大利”(Vitis vinifera L.)葡萄品种的差异遗传稳定性
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183301066
S. H. Z. Maia, S. A. Oliveira-Collet, C. A. Mangolin, M. F. Machado
During more than 50 years the vegetative propagation has been the form of maintaining and multiplication of the cv. ’Italy’ vineyards, a ’Bicane’ x ’Muscat Hamburg’ hybrid. In the current study, polymorphism in 17 microsatellite loci was used to evaluate the genetic stability at DNA level in vineyards of cv. ’Italy’ planted in different regions of the states of Parana and Sao Paulo, Brazil. Unchanged and equal allele frequency indicating genetic stability was reported in 47% of the microsatellite loci in vineyards of six localities, while allele frequency variation has been observed in Scu15vv, Udv44, Udv74, Udv96, Udv107, Udv108, Vvmd5, Vvmd6 and Vvs3 microsatellite loci. Alleles Udv96 140 and Vvs3 448 , detected in vines in only one of the vineyards, evidenced somatic mutations at molecular level in cv. ’Italy’. Genetic diversity, as result of changes in the allele frequencies in 53% of microsatellite loci, was detected more frequently than somatic mutations due to new alleles. Polymorphism in microsatellite loci revealed different genetic stability in vineyards of cv. ’Italy’ cultivated in six different Brazilian regions and indicated vineyards with less genetic stability as a possible source of somatic mutants, showing traits of agronomic interest with a potential to generate new cultivars.
在过去的50多年里,无性繁殖一直是维持和繁殖变异的一种方式。“意大利”葡萄园,“比堪”和“马斯喀特汉堡”的杂交品种。本研究利用17个微卫星位点的多态性,在DNA水平上对葡萄的遗传稳定性进行了评价。“意大利”种植在巴西巴拉那州和圣保罗州的不同地区。在6个地区的葡萄园中,47%的微卫星位点的等位基因频率不变且相同,表明遗传稳定性,而在Scu15vv、Udv44、Udv74、Udv96、Udv107、Udv108、Vvmd5、Vvmd6和Vvs3微卫星位点的等位基因频率发生了变化。等位基因Udv96 140和Vvs3 448仅在一个葡萄园中检测到,在分子水平上证明了体细胞突变。“意大利”。遗传多样性是53%微卫星位点等位基因频率变化的结果,比新等位基因引起的体细胞突变更频繁地被检测到。微卫星位点的多态性揭示了葡萄品种遗传稳定性的差异。“意大利”在巴西六个不同的地区种植,并指出遗传稳定性较差的葡萄园可能是体细胞突变的来源,显示出具有产生新品种潜力的农艺性状。
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引用次数: 4
Estimation of daily mean temperatures: an accurate method for the Douro Valley 每日平均温度的估计:杜罗河河谷的一种精确方法
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183302167
António C. Real, J. Borges, Carlos A. S. Oliveira
Air temperature data from many locations worldwide are only available as series of daily minima and maxima temperatures. Historically, several different approaches have been used to estimate the actual daily mean temperature, as only in the last two or three decades automatic thermometers are able to compute its actual value. The most common approach is to estimate it by averaging the daily minima and maxima. When only daily minima and maxima are available, an alternative approach, proposed by Dall’Amico and Hornsteiner in 2006, uses the two daily extremes together with next day minima temperature and a coefficient related to the local daily astronomical sunset time. Additionally, the method uses two optimizable coefficients related to the region’s temperature profile. In order to use this approach it is necessary to optimize the region’s unknown parameters. For this optimization, it is necessary a dataset containing the maxima, minima, and the actual daily mean temperatures for at least one year. In this research, for the period 2007-2014, we used three datasets of minima, maxima and actual mean temperatures obtained at three automatic meteorological stations located in the Douro Valley to optimize the two unknown parameters in the Dall’Amico and Hornsteiner approach. Moreover, we compared the actual mean daily temperatures available from the three datasets with the correspondent values estimated by using i) the usual approach of averaging the daily maxima and minima temperatures and ii) the Dall’Amico and Hornsteiner approach. Results show that the former approach overestimates, on average, the daily mean temperatures by 0.5oC. The Dall’Amico and Hornsteiner approach showed to be a better approximation of mean temperatures for the three meteorological stations used in this research, being unbiased relative to the actual mean values of daily temperatures. In conclusion, this research confirms that the Dall’Amico and Hornsteiner is a better approach to estimate the mean daily temperatures and provides the optimized parameters for three sites located at each of the three sub-regions of the Douro Valley (Baixo Corgo, Cima Corgo and Douro Superior ).
世界上许多地方的气温数据只有每日最低和最高气温的系列。历史上,有几种不同的方法被用来估计实际的日平均温度,因为只有在过去的二三十年里,自动温度计才能计算出它的实际值。最常见的方法是通过每日最小值和最大值的平均值来估计它。当只有日极小值和日极大值可用时,Dall 'Amico和Hornsteiner在2006年提出了另一种方法,将两个日极值与第二天的最低温度和与当地日天文日落时间相关的系数一起使用。此外,该方法使用两个与该地区的温度分布相关的可优化系数。为了使用这种方法,有必要对区域的未知参数进行优化。对于这种优化,需要一个包含至少一年的最大值、最小值和实际日平均温度的数据集。本研究利用2007-2014年Douro流域3个自动气象站的最小、最高和实际平均气温数据集,对Dall 'Amico和Hornsteiner方法中的两个未知参数进行了优化。此外,我们将三个数据集的实际日平均温度与常用的日最高和最低温度平均方法和Dall 'Amico和Hornsteiner方法估计的相应值进行了比较。结果表明,前一种方法平均高估日平均气温0.5℃。Dall 'Amico和Hornsteiner方法表明,对于本研究中使用的三个气象站的平均温度,Dall 'Amico和Hornsteiner方法是一个更好的近似值,相对于每日温度的实际平均值是无偏的。综上所述,本研究证实了Dall 'Amico和Hornsteiner是较好的日平均温度估算方法,并为Douro Valley三个子区域(Baixo Corgo, Cima Corgo和Douro Superior)的三个站点提供了优化参数。
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引用次数: 0
Use of hydrogen peroxide, citric acid and sodium hypochlorite as sanitizer for minimally processed table grapes 使用过氧化氢、柠檬酸和次氯酸钠作为最低限度加工的鲜食葡萄的消毒剂
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183301058
M. Ergun, Ezgi Dogan
The objective of this study was to explore two local table grape cultivars, one is white and the other one red colored, as a minimally processed produce, and to identify the most effective sanitizers (hydrogen peroxide, citric acid or sodium hypochlorite) to control microbial growth. The table grape cultivars indigenous to Elazig province of Turkey ‘Agin Beyazi’ and ‘Agin Kirmizisi’ were tested in the present experiment. Grape clusters were washed by dipping in tap water as control, in citric acid (20 g/L), with sodium hypochlorite (50 mg/L), or with hydrogen peroxide (20 g/L), solutions for 1 minute. Grape berries were then placed into PET clamshells and kept at 4 °C for 10 days. The berries were subject to quality assessments during the storage, and to total aerobic microbial and fungal count at the end. Washing with hydrogen peroxide- or sodium hypochlorite-diluted water was very effective controlling both aerobic microbial and fungal growth. Citric acid treatment however represented no significant effect on microbial growth. It is concluded that both hydrogen peroxide and sodium hypochlorite are strong sanitizers for table grapes tested, with no detection of undesirable effects.
本研究的目的是探索两种当地的食用葡萄品种,一种是白色的,另一种是红色的,作为最低限度的加工产品,并确定最有效的杀菌剂(过氧化氢、柠檬酸或次氯酸钠)来控制微生物的生长。本试验以土耳其Elazig省的食用葡萄品种“Agin Beyazi”和“Agin Kirmizisi”为试验材料。葡萄串以自来水为对照,在柠檬酸(20 g/L)、次氯酸钠(50 mg/L)或过氧化氢(20 g/L)溶液中浸泡1分钟。然后将葡萄浆果放入PET蛤壳中,在4°C下保存10天。在贮藏期间对这些浆果进行质量评估,并在贮藏结束时对好氧微生物和真菌总数进行评估。用过氧化氢或次氯酸钠稀释的水清洗,对控制好氧微生物和真菌的生长都非常有效。柠檬酸处理对微生物生长无显著影响。结果表明,双氧水和次氯酸钠对鲜食葡萄均为强效杀菌剂,未发现不良反应。
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引用次数: 2
Phenolic composition and total antioxidant capacity analysis of red wine vinegars commercialized in Portuguese market 葡萄牙市售红酒醋酚类成分及总抗氧化能力分析
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183302102
M. Cruz, A. C. Correia, F. Gonçalves, A. Jordão
In the last years, there has been an increase in consumption of wine vinegars in Portugal. Thus, the aim of this work was to evaluate the phenolic composition and total antioxidant capacity from several commercial red wine vinegars commercialized in Portuguese market. Several parameters were evaluated: general phenolic composition, chromatic characteristics, individual anthocyanins and phenolic acids by HPLC, and total antioxidant capacity by two methodologies (DPPH and ABTS). For the different parameters analyzed, the red wine vinegars samples studied differed significantly. Vinegars with higher phenolic content tend to have lower lightness, but higher values of the red component color. High diversity of anthocyanins was detected, with some of the vinegars being distinguished by having significantly higher values of anthocyanins compared to the others, as was detected for the generality of the other phenolic parameters. The total antioxidant capacity was positively correlated with the different phenolic parameters. Finally, higher total antioxidant capacity was detected for the phenolic fraction containing anthocyanins and polymeric proanthocyanidins. The results obtained confirm that red wine vinegars are good sources of phenolic compounds and antioxidants. However, there is a great diversity of values for the various red wine vinegars commercialized in the Portuguese market.
在过去的几年里,葡萄牙葡萄酒醋的消费量有所增加。因此,本研究的目的是评价几种在葡萄牙市场上销售的商业红酒醋的酚类成分和总抗氧化能力。用高效液相色谱法评价了总酚类成分、颜色特征、单个花青素和酚酸,用DPPH和ABTS两种方法评价了总抗氧化能力。对于不同的分析参数,所研究的红酒醋样品差异显著。酚醛含量高的醋往往亮度较低,但红色成分颜色值较高。检测到花青素的高度多样性,与其他醋相比,一些醋的花青素含量明显更高,因为检测到其他酚类参数的普遍性。总抗氧化能力与不同的酚类参数呈正相关。最后,发现含有花青素和聚合原花青素的酚类组分具有较高的总抗氧化能力。结果证实,红酒醋是酚类化合物和抗氧化剂的良好来源。然而,在葡萄牙市场上商业化的各种红酒醋的价值差异很大。
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引用次数: 8
Molecular characterization of berry color locus on the portuguese cv. ‘Fernão Pires’ and cv. ‘Verdelho’ and their red-berried somatic variant cultivars 葡萄牙葡萄果实颜色位点的分子特征。“fern<s:1> o皮雷斯”和简历。‘Verdelho’及其红莓体变品种
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183302184
V. Ferreira, I. Castro, D. Carrasco, O. Pinto-Carnide, R. Arroyo-García
Genotyping studies are increasing the knowledge on grapevine biodiversity, particularly regarding grape berry skin color somatic variants, supporting the research on the color trait. This study aimed to evaluate the effect of the berry color locus, and its surrounding genomic region, on the color variation of the Portuguese white-skinned cultivars ‘Fernao Pires’ and ‘Verdelho’ and its derived red-berried somatic variants cv. ‘Fernao Pires Rosado’ and cv. ‘Verdelho Roxo’, respectively.The analysis of Gret1 insertion within the VvMYBA1 gene revealed no polymorphism responsible for white-to-red shift of the red-skinned cv. ‘Fernao Pires Rosado’ and cv. ‘Verdelho Roxo’. Moreover, VvMYBA2 showed an important role regarding the phenotypic variation of cv. ‘Fernao Pires’, through the recovery of the functional allele G on cv. ‘Fernao Pires Rosado’. Regarding the data obtained for cv. ‘Verdelho’ and cv. ‘Verdelho Roxo’, both cultivars showed Gret1 insertion on VvMYBA1 and non-functional T allele on VvMYBA2 in homozygosity for both cell layers of shoot apical meristem, suggesting the occurrence of other mutational events responsible for the color gain.
基因分型研究增加了人们对葡萄生物多样性的认识,特别是对葡萄果实表皮颜色体细胞变异的认识,为葡萄颜色性状的研究提供了支持。本研究旨在评估浆果颜色位点及其周围基因组区域对葡萄牙白皮品种费尔诺皮雷(Fernao Pires)和Verdelho (Verdelho)及其衍生红莓体细胞变体cv的颜色变异的影响。“费尔诺·皮雷·罗萨多”和简历。分别是Verdelho Roxo。对VvMYBA1基因中Gret1插入的分析显示,没有导致红皮肤cv白向红转移的多态性。“费尔诺·皮雷·罗萨多”和简历。“Verdelho Roxo”。此外,VvMYBA2在cv表型变异中发挥了重要作用。“费尔诺皮雷”,通过恢复cv上的功能等位基因G。“费尔诺·皮雷·罗萨多”。关于cv的数据。“Verdelho”和cv。‘Verdelho Roxo’和‘Verdelho Roxo’的茎尖分生组织两层细胞的纯合性都显示了VvMYBA1上的Gret1插入和VvMYBA2上的无功能T等位基因,表明发生了其他导致颜色增加的突变事件。
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引用次数: 0
Leaf morphoanatomy traits in white grapevine cultivars with distinct geographical origin 地理来源不同的白葡萄品种叶片形态解剖学特征
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2018-01-01 DOI: 10.1051/CTV/20183301090
G. Teixeira, A. Monteiro, C. Santos, C. Lopes
This study aims to compare the leaf morphoanatomical characteristics of seven Vitis vinifera subsp. vinifera white cultivars with different geographical origin, grown side by side at the same “terroir”. The leaf morphoanatomical traits analyzed under light and scanning electron microscopy (SEM) showed large variability among genotypes. Significant differences between cultivars were observed in single leaf area, specific leaf area, leaf density and in the thickness of cuticle, upper and lower epidermal cells and mesophyll. Leaf dry mass per area presented a significant positive correlation with leaf density but showed no correlation with leaf thickness, results that can be explained by the quite different mesophyll structure, epidermal cells and cuticle thickness. ‘Alvarinho’, ‘Encruzado’, ‘Macabeu’ and ‘Viosinho’ were the cultivars with the highest thickness of cuticle and mesophyll tissues. Under SEM magnification three types of stomata were identified: sunken, at the same level and raised above, with the same level type presenting the higher percentage in all cultivars. Stomata density presented significant differences between cultivars, with ‘Macabeu’ showing the highest value and ‘Alvarinho’ and ‘Arinto’ the lowest ones. The hairs on the lower surface presented a similar woolly aspect in all cultivars. Calcium oxalate crystals, raphids and druses were visible and widely distributed in the parenchyma tissues. The observed differences in leaf traits among genotypes suggest a major role of leaf anatomy in determining grapevine capacity for coping with different environmental conditions.
本研究旨在比较7种葡萄亚种的叶片形态解剖特征。不同地理来源的白葡萄品种,并排生长在同一“风土”。在光镜和扫描电镜下分析的叶片形态解剖性状显示,基因型间存在较大差异。品种间单叶面积、比叶面积、叶密度以及角质层、上下表皮细胞和叶肉的厚度均存在显著差异。每面积叶干质量与叶密度呈显著正相关,而与叶厚度无显著相关,这与叶肉结构、表皮细胞和角质层厚度差异较大有关。“Alvarinho”、“Encruzado”、“Macabeu”和“Viosinho”是角质层和叶肉组织厚度最大的品种。在扫描电镜下,发现了三种类型的气孔:凹陷型、同水平型和高于水平型,其中同水平型在所有品种中所占比例都较高。不同品种间气孔密度差异显著,‘Macabeu’气孔密度最高,‘Alvarinho’和‘Arinto’气孔密度最低。在所有品种中,下表面的毛呈现相似的毛状外形。实质组织中可见草酸钙晶体、结晶和结节,分布广泛。观察到的不同基因型叶片性状的差异表明,叶片解剖结构在决定葡萄应对不同环境条件的能力方面起着重要作用。
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引用次数: 7
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Ciencia E Tecnica Vitivinicola
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