Neurophotonics最新文献

{"title":"Mapping multi-regional functional connectivity of astrocyte-neuronal networks during behaviors.","authors":"Haoyu Wang, Mingzhu Huang, Shaofan Yang, Jiameng Xu, Jin Li, Han Qin, Shanshan Liang, Teng Teng, Chuanyan Yang, Mingyue Gong, Yong He, Xingyi Li, Huiquan Wang, Xiang Liao, Xiaowei Chen, Zhiqi Yang, Kuan Zhang","doi":"10.1117/1.NPh.11.4.045010","DOIUrl":"https://doi.org/10.1117/1.NPh.11.4.045010","url":null,"abstract":"<p><strong>Significance: </strong>Diverse behaviors rely on coordinated activity and multi-regional functional connectivity within astrocyte-neuronal networks. However, current techniques for simultaneously measuring astrocytic and neuronal activities across multiple brain regions during behaviors remain limited.</p><p><strong>Aim: </strong>We propose a multi-fiber solution that can simultaneously record activities of astrocyte-neuronal networks across multiple regions during behaviors.</p><p><strong>Approach: </strong>We employed cell-specific dual-color genetically encoded calcium indicators (GECIs) and multi-fiber photometry to simultaneously measure astrocytic and neuronal Ca²⁺ transients across multiple brain regions in freely behaving animals.</p><p><strong>Results: </strong>Our findings demonstrate that both movements and sensory stimuli induce synchronized and highly correlated Ca²⁺ transients in astrocytes and neurons of freely behaving mice. In addition, we recorded astrocytic and neuronal Ca²⁺ transients from multiple brain regions during mouse behaviors. Our observations reveal heightened synchronization of astrocytic and neuronal Ca²⁺ transients across different brain regions during movements or sensory stimuli, indicating enhanced functional connectivity within brain-wide astrocyte-neuronal networks.</p><p><strong>Conclusions: </strong>Multi-fiber photometry, combined with cell-specific dual-color GECIs, represents a powerful approach for investigating astrocytic and neuronal activities across different brain regions during behaviors. This technique serves as a versatile tool for analyzing the multi-regional functional connectivity map of astrocyte-neuronal networks associated with specific behaviors.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerebral baseline optical and hemodynamic properties in pediatric population: a large cohort time-domain near-infrared spectroscopy study.","authors":"Valeria Calcaterra, Michele Lacerenza, Caterina Amendola, Mauro Buttafava, Davide Contini, Virginia Rossi, Lorenzo Spinelli, Sara Zanelli, Gianvincenzo Zuccotti, Alessandro Torricelli","doi":"10.1117/1.NPh.11.4.045009","DOIUrl":"https://doi.org/10.1117/1.NPh.11.4.045009","url":null,"abstract":"<p><strong>Significance: </strong>Reference cerebral near-infrared spectroscopy (NIRS) data on the pediatric population are scarce, and in most cases, only cerebral oxygen saturation ( <math> <mrow> <msub><mrow><mi>SO</mi></mrow> <mrow><mn>2</mn></mrow> </msub> </mrow> </math> ) measured by continuous wave spatially resolved spectroscopy NIRS is reported. Absolute data for baseline optical and hemodynamic parameters are missing.</p><p><strong>Aim: </strong>We aimed at collecting baseline cerebral optical parameters [absorption coefficient, <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> ; reduced scattering coefficient, <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> ; differential pathlength factor (DPF)] and hemodynamic parameters [oxy-hemoglobin content ( <math> <mrow> <msub><mrow><mi>HbO</mi></mrow> <mrow><mn>2</mn></mrow> </msub> </mrow> </math> ), deoxyhemoglobin content (HHb), total hemoglobin content (tHB), <math> <mrow> <msub><mrow><mi>SO</mi></mrow> <mrow><mn>2</mn></mrow> </msub> </mrow> </math> ] in a large cohort of pediatric patients. The objectives are to establish reference optical values in this population and evaluate the reproducibility of a commercial time domain (TD) NIRS tissue oximeter.</p><p><strong>Approach: </strong>TD NIRS measurements were performed in the prefrontal cortex at 686 and 830 nm with a 2.5-cm source-detector distance and 1-Hz acquisition rate. Five independent measurements (after probe replacement) were taken for every subject. TD NIRS data were fitted to a photon diffusion model to estimate the optical parameters. From the absorption coefficients, the hemodynamic parameters were derived by Beer's law. Auxological and physiological information was also collected to explore the potential correlations with NIRS data.</p><p><strong>Results: </strong>We measured 305 patients in the age range of 2 to 18 years. Absolute values for baseline optical and hemodynamic parameters were shown as a function of age and auxological variables. From the analysis of the repositioning after probe replacement, the time-domain near-infrared spectroscopy device exhibited an average precision (intended as coefficient of variation) of <math><mrow><mo><</mo> <mn>5</mn> <mo>%</mo></mrow> </math> for <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> , DPF, <math> <mrow><msub><mi>HbO</mi> <mn>2</mn></msub> </mrow> </math> , HHb, and tHb, whereas precision was <math><mrow><mo><</mo> <mn>2</mn> <mo>%</mo></mrow> </math> for <math> <mrow><msub><mi>SO</mi> <mn>2</mn></msub> </mrow> </math> .</p><p><strong>Conclusions: </strong>We provided baseline values for optical and hemodynamic parameters in a large cohort of healthy pediatric subjects with good precision, providing a foundation for future investigations into clinically relevant deviations in these parameters.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}