首页 > 最新文献

Neurophotonics最新文献

英文 中文
Two-dimensional electro-optical multiphoton microscopy. 二维电子光学多光子显微镜。
IF 4.8 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-06-05 DOI: 10.1117/1.NPh.11.2.025005
Deano M Farinella, Samuel Stanek, Harishankar Jayakumar, Zachary L Newman, Jacob Gable, James Leger, Aaron Kerlin

Significance: The development of genetically encoded fluorescent indicators of neural activity with millisecond dynamics has generated demand for ever faster two-photon (2P) imaging systems, but acoustic and mechanical beam scanning technologies are approaching fundamental limits. We demonstrate that potassium tantalate niobate (KTN) electro-optical deflectors (EODs), which are not subject to the same fundamental limits, are capable of ultrafast two-dimensional (2D) 2P imaging in vivo.

Aim: To determine if KTN-EODs are suitable for 2P imaging, compatible with 2D scanning, and capable of ultrafast in vivo imaging of genetically encoded indicators with millisecond dynamics.

Approach: The performance of a commercially available KTN-EOD was characterized across a range of drive frequencies and laser parameters relevant to in vivo 2P microscopy. A second KTN-EOD was incorporated into a dual-axis scan module, and the system was validated by imaging signals in vivo from ASAP3, a genetically encoded voltage indicator.

Results: Optimal KTN-EOD deflection of laser light with a central wavelength of 960 nm was obtained up to the highest average powers and pulse intensities tested (power: 350 mW; pulse duration: 118 fs). Up to 32 resolvable spots per line at a 560 kHz line scan rate could be obtained with single-axis deflection. The complete dual-axis EO 2P microscope was capable of imaging a 13  μm by 13  μm field-of-view at over 10 kHz frame rate with 0.5  μm lateral resolution. We demonstrate in vivo imaging of neurons expressing ASAP3 with high temporal resolution.

Conclusions: We demonstrate the suitability of KTN-EODs for ultrafast 2P cellular imaging in vivo, providing a foundation for future high-performance microscopes to incorporate emerging advances in KTN-based scanning technology.

意义重大:具有毫秒级动态的神经活动基因编码荧光指示器的开发,催生了对更快的双光子(2P)成像系统的需求,但声学和机械光束扫描技术已接近基本极限。我们证明,钽酸铌酸钾(KTN)电子光学偏转器(EOD)不受同样的基本限制,能够在体内进行超快二维(2D)双光子成像。目的:确定 KTN-EOD 是否适用于双光子成像,是否与二维扫描兼容,是否能够在体内对具有毫秒级动态的基因编码指标进行超快成像:方法:在与活体 2P 显微镜相关的驱动频率和激光参数范围内,对商用 KTN-EOD 的性能进行了鉴定。在双轴扫描模块中加入了第二个 KTN-EOD,并通过对基因编码电压指示器 ASAP3 的体内信号成像对系统进行了验证:结果:在测试的最高平均功率和脉冲强度(功率:350 mW;脉冲持续时间:118 fs)条件下,中心波长为 960 nm 的 KTN-EOD 可获得最佳激光偏转。在 560 kHz 的行扫描速率下,单轴偏转最多可获得每行 32 个可分辨光斑。完整的双轴 EO 2P 显微镜能够以超过 10 kHz 的帧速率对 13 μm x 13 μm 的视场进行成像,横向分辨率为 0.5 μm。我们展示了表达 ASAP3 的神经元在体内的高时间分辨率成像:我们证明了 KTN-EODs 适用于体内超快 2P 细胞成像,为未来高性能显微镜采用基于 KTN 的新兴扫描技术奠定了基础。
{"title":"Two-dimensional electro-optical multiphoton microscopy.","authors":"Deano M Farinella, Samuel Stanek, Harishankar Jayakumar, Zachary L Newman, Jacob Gable, James Leger, Aaron Kerlin","doi":"10.1117/1.NPh.11.2.025005","DOIUrl":"10.1117/1.NPh.11.2.025005","url":null,"abstract":"<p><strong>Significance: </strong>The development of genetically encoded fluorescent indicators of neural activity with millisecond dynamics has generated demand for ever faster two-photon (2P) imaging systems, but acoustic and mechanical beam scanning technologies are approaching fundamental limits. We demonstrate that potassium tantalate niobate (KTN) electro-optical deflectors (EODs), which are not subject to the same fundamental limits, are capable of ultrafast two-dimensional (2D) 2P imaging <i>in vivo</i>.</p><p><strong>Aim: </strong>To determine if KTN-EODs are suitable for 2P imaging, compatible with 2D scanning, and capable of ultrafast <i>in vivo</i> imaging of genetically encoded indicators with millisecond dynamics.</p><p><strong>Approach: </strong>The performance of a commercially available KTN-EOD was characterized across a range of drive frequencies and laser parameters relevant to <i>in vivo</i> 2P microscopy. A second KTN-EOD was incorporated into a dual-axis scan module, and the system was validated by imaging signals <i>in vivo</i> from ASAP3, a genetically encoded voltage indicator.</p><p><strong>Results: </strong>Optimal KTN-EOD deflection of laser light with a central wavelength of 960 nm was obtained up to the highest average powers and pulse intensities tested (power: 350 mW; pulse duration: 118 fs). Up to 32 resolvable spots per line at a 560 kHz line scan rate could be obtained with single-axis deflection. The complete dual-axis EO 2P microscope was capable of imaging a <math><mrow><mn>13</mn><mtext>  </mtext><mi>μ</mi><mi>m</mi></mrow></math> by <math><mrow><mn>13</mn><mtext>  </mtext><mi>μ</mi><mi>m</mi></mrow></math> field-of-view at over 10 kHz frame rate with <math><mrow><mo>∼</mo><mn>0.5</mn><mtext>  </mtext><mi>μ</mi><mi>m</mi></mrow></math> lateral resolution. We demonstrate <i>in vivo</i> imaging of neurons expressing ASAP3 with high temporal resolution.</p><p><strong>Conclusions: </strong>We demonstrate the suitability of KTN-EODs for ultrafast 2P cellular imaging <i>in vivo</i>, providing a foundation for future high-performance microscopes to incorporate emerging advances in KTN-based scanning technology.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"025005"},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11151658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141263228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using spectral derivatives to remove the influence of hair on optical images of the static absorbing properties of tissue-like turbid media. 利用光谱衍生物消除毛发对组织样浑浊介质静态吸收特性光学图像的影响。
IF 5.3 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-04-26 DOI: 10.1117/1.NPh.11.2.025002
Jeremy C Hebden, Gianna Forrester, Haoyang Zhang, Danica M Pacis

Significance: Although measurements of near-infrared light diffusely reflected from the head and other biological tissues are commonly used to generate images revealing changes in the concentrations of oxy- and deoxy-hemoglobin, static imaging of absolute concentrations has been inhibited by the unknown and variable coupling between the optical probe and the skin, to which hair is often a significant contributor. Measurements of spectral derivatives provide a means of overcoming this shortcoming.

Aim: The aim is to demonstrate experimentally that measurements of the derivative of the attenuation of the detected signal with respect to wavelength can be used to achieve images that are immune to the spatial variation of hair on the surface. The objective is to generate topographic images representative of static absorbing properties rather than retrieving absolute optical coefficients, which requires a tomographic approach.

Approach: The surface of a tissue-equivalent phantom, containing targets with different concentrations of absorbing dye, was coated with a layer of dark hair. The phantom was then imaged using a broadband source and spectrometer, and prior knowledge of the absorbing characteristics of the dye and of melanin was used to acquire separate images of each.

Results: The targets within the phantom are revealed with remarkable clarity, although a nonlinear relationship between the target contrast and absorption was observed. This nonlinear behavior was confirmed and explained using a Monte Carlo model of light propagation in a slab of similar absorbing properties.

Conclusions: A spectral derivative approach could be an effective tool for in vivo topographic imaging of the static optical properties of the brain and other tissues, avoiding the deleterious effects of hair.

意义重大:尽管测量头部和其他生物组织漫反射的近红外光通常用于生成显示氧合血红蛋白和脱氧血红蛋白浓度变化的图像,但由于光学探头与皮肤之间存在未知和可变的耦合,而头发通常是造成耦合的重要因素,因此绝对浓度的静态成像一直受到抑制。目的:目的是通过实验证明,通过测量检测信号衰减与波长的导数,可以获得不受表面毛发空间变化影响的图像。目的是生成代表静态吸收特性的地形图像,而不是检索绝对光学系数,后者需要采用层析方法:方法:在含有不同吸收染料浓度目标的组织等效模型表面涂上一层深色毛发。然后使用宽带光源和光谱仪对该模型进行成像,并利用事先了解的染料和黑色素的吸收特性,分别获取各自的图像:结果:虽然观察到目标对比度和吸收之间存在非线性关系,但模型中的目标显示得非常清晰。使用具有类似吸收特性的平板中光传播的蒙特卡洛模型证实并解释了这种非线性行为:光谱导数法是对大脑和其他组织的静态光学特性进行活体拓扑成像的有效工具,可避免毛发的有害影响。
{"title":"Using spectral derivatives to remove the influence of hair on optical images of the static absorbing properties of tissue-like turbid media.","authors":"Jeremy C Hebden, Gianna Forrester, Haoyang Zhang, Danica M Pacis","doi":"10.1117/1.NPh.11.2.025002","DOIUrl":"https://doi.org/10.1117/1.NPh.11.2.025002","url":null,"abstract":"<p><strong>Significance: </strong>Although measurements of near-infrared light diffusely reflected from the head and other biological tissues are commonly used to generate images revealing changes in the concentrations of oxy- and deoxy-hemoglobin, static imaging of absolute concentrations has been inhibited by the unknown and variable coupling between the optical probe and the skin, to which hair is often a significant contributor. Measurements of spectral derivatives provide a means of overcoming this shortcoming.</p><p><strong>Aim: </strong>The aim is to demonstrate experimentally that measurements of the derivative of the attenuation of the detected signal with respect to wavelength can be used to achieve images that are immune to the spatial variation of hair on the surface. The objective is to generate topographic images representative of static absorbing properties rather than retrieving absolute optical coefficients, which requires a tomographic approach.</p><p><strong>Approach: </strong>The surface of a tissue-equivalent phantom, containing targets with different concentrations of absorbing dye, was coated with a layer of dark hair. The phantom was then imaged using a broadband source and spectrometer, and prior knowledge of the absorbing characteristics of the dye and of melanin was used to acquire separate images of each.</p><p><strong>Results: </strong>The targets within the phantom are revealed with remarkable clarity, although a nonlinear relationship between the target contrast and absorption was observed. This nonlinear behavior was confirmed and explained using a Monte Carlo model of light propagation in a slab of similar absorbing properties.</p><p><strong>Conclusions: </strong>A spectral derivative approach could be an effective tool for <i>in vivo</i> topographic imaging of the static optical properties of the brain and other tissues, avoiding the deleterious effects of hair.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"025002"},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11046095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
BioLuminescent OptoGenetics in the choroid plexus: integrated opto- and chemogenetic control in vivo. 脉络丛中的生物发光光遗传学:体内光遗传学和化学遗传学的综合控制。
IF 4.8 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-06-28 DOI: 10.1117/1.NPh.11.2.024210
Eric Klein, Sophie Marsh, Jordan Becker, Mark Andermann, Maria Lehtinen, Christopher I Moore

Significance: The choroid plexus (ChP) epithelial network displays diverse dynamics, including propagating calcium waves and individuated fluctuations in single cells. These rapid events underscore the possibility that ChP dynamics may reflect behaviorally relevant and clinically important changes in information processing and signaling. Optogenetic and chemogenetic tools provide spatiotemporally precise and sustained approaches for testing such dynamics in vivo. Here, we describe the feasibility of a novel combined opto- and chemogenetic tool, BioLuminescent-OptoGenetics (BL-OG), for the ChP in vivo. In the "LuMinOpsin" (LMO) BL-OG strategy, a luciferase is tethered to an adjacent optogenetic element. This molecule allows chemogenetic activation when the opsin is driven by light produced through luciferase binding a small molecule (luciferin) or by conventional optogenetic light sources and BL-OG report of activation through light production.

Aim: To test the viability of BL-OG/LMO for ChP control.

Approach: Using transgenic and Cre-directed targeting to the ChP, we expressed LMO3 (a Gaussia luciferase-VChR1 fusion), a highly effective construct in neural systems. In mice expressing LMO3 in ChP, we directly imaged BL light production following multiple routes of coelenterazine (CTZ: luciferin) administration using an implanted cannula system. We also used home-cage videography with Deep LabCut analysis to test for any impact of repeated CTZ administration on basic health and behavioral indices.

Results: Multiple routes of CTZ administration drove BL photon production, including intracerebroventricular, intravenous, and intraperitoneal injection. Intravenous administration resulted in fast "flash" kinetics that diminished in seconds to minutes, and intraperitoneal administration resulted in slow rising activity that sustained hours. Mice showed no consistent impact of 1 week of intraperitoneal CTZ administration on weight, drinking, motor behavior, or sleep/wake cycles.

Conclusions: BL-OG/LMO provides unique advantages for testing the role of ChP dynamics in biological processes.

意义重大:脉络丛(ChP)上皮细胞网络显示出多种多样的动态,包括传播的钙波和单细胞中的个别波动。这些快速事件突出表明,脉络丛上皮细胞的动态可能反映了信息处理和信号传导中与行为相关且具有临床重要性的变化。光遗传学和化学遗传学工具提供了时空精确和持续的方法来测试体内的这种动态变化。在这里,我们描述了一种新型光遗传学和化学遗传学组合工具--生物发光-光遗传学(BioLuminescent-OptoGenetics,BL-OG)--在体内用于 ChP 的可行性。在 "LuMinOpsin"(LMO)BL-OG 策略中,荧光素酶与相邻的光遗传元件相连。当通过荧光素酶结合小分子(荧光素)产生的光或通过传统光遗传光源和 BL-OG 报告通过产生的光激活时,该分子可实现化学激活:我们利用转基因和Cre定向靶向ChP,表达了LMO3(一种高斯荧光素酶-VChR1融合物),这是一种在神经系统中非常有效的构建物。在 ChP 中表达 LMO3 的小鼠中,我们使用植入插管系统直接成像了多种途径给予腔肠素(CTZ:荧光素)后产生的 BL 光。我们还利用家笼录像和Deep LabCut分析检测了重复给药CTZ对基本健康和行为指数的影响:结果:CTZ的多种给药途径都能促进BL光子的产生,包括脑静脉注射、静脉注射和腹腔注射。静脉注射会产生快速的 "闪光 "动力学,并在数秒至数分钟内减弱;腹腔注射会产生持续数小时的缓慢上升活动。小鼠腹腔注射 CTZ 1 周后,体重、饮水、运动行为或睡眠/觉醒周期均无明显变化:结论:BL-OG/LMO 为测试 ChP 动态在生物过程中的作用提供了独特的优势。
{"title":"BioLuminescent OptoGenetics in the choroid plexus: integrated opto- and chemogenetic control <i>in vivo</i>.","authors":"Eric Klein, Sophie Marsh, Jordan Becker, Mark Andermann, Maria Lehtinen, Christopher I Moore","doi":"10.1117/1.NPh.11.2.024210","DOIUrl":"10.1117/1.NPh.11.2.024210","url":null,"abstract":"<p><strong>Significance: </strong>The choroid plexus (ChP) epithelial network displays diverse dynamics, including propagating calcium waves and individuated fluctuations in single cells. These rapid events underscore the possibility that ChP dynamics may reflect behaviorally relevant and clinically important changes in information processing and signaling. Optogenetic and chemogenetic tools provide spatiotemporally precise and sustained approaches for testing such dynamics <i>in vivo</i>. Here, we describe the feasibility of a novel combined opto- and chemogenetic tool, BioLuminescent-OptoGenetics (BL-OG), for the ChP <i>in vivo</i>. In the \"LuMinOpsin\" (LMO) BL-OG strategy, a luciferase is tethered to an adjacent optogenetic element. This molecule allows chemogenetic activation when the opsin is driven by light produced through luciferase binding a small molecule (luciferin) or by conventional optogenetic light sources and BL-OG report of activation through light production.</p><p><strong>Aim: </strong>To test the viability of BL-OG/LMO for ChP control.</p><p><strong>Approach: </strong>Using transgenic and Cre-directed targeting to the ChP, we expressed LMO3 (a <i>Gaussia</i> luciferase-VChR1 fusion), a highly effective construct in neural systems. In mice expressing LMO3 in ChP, we directly imaged BL light production following multiple routes of coelenterazine (CTZ: luciferin) administration using an implanted cannula system. We also used home-cage videography with Deep LabCut analysis to test for any impact of repeated CTZ administration on basic health and behavioral indices.</p><p><strong>Results: </strong>Multiple routes of CTZ administration drove BL photon production, including intracerebroventricular, intravenous, and intraperitoneal injection. Intravenous administration resulted in fast \"flash\" kinetics that diminished in seconds to minutes, and intraperitoneal administration resulted in slow rising activity that sustained hours. Mice showed no consistent impact of 1 week of intraperitoneal CTZ administration on weight, drinking, motor behavior, or sleep/wake cycles.</p><p><strong>Conclusions: </strong>BL-OG/LMO provides unique advantages for testing the role of ChP dynamics in biological processes.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024210"},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11213259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seizure event detection using intravital two-photon calcium imaging data. 利用眼内双光子钙成像数据检测癫痫发作事件。
IF 4.8 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-01-25 DOI: 10.1117/1.NPh.11.2.024202
Matthew A Stern, Eric R Cole, Robert E Gross, Ken Berglund

Significance: Intravital cellular calcium imaging has emerged as a powerful tool to investigate how different types of neurons interact at the microcircuit level to produce seizure activity, with newfound potential to understand epilepsy. Although many methods exist to measure seizure-related activity in traditional electrophysiology, few yet exist for calcium imaging.

Aim: To demonstrate an automated algorithmic framework to detect seizure-related events using calcium imaging-including the detection of pre-ictal spike events, propagation of the seizure wavefront, and terminal spreading waves for both population-level activity and that of individual cells.

Approach: We developed an algorithm for precise recruitment detection of population and individual cells during seizure-associated events, which broadly leverages averaged population activity and high-magnitude slope features to detect single-cell pre-ictal spike and seizure recruitment. We applied this method to data recorded using awake in vivo two-photon calcium imaging during pentylenetetrazol-induced seizures in mice.

Results: We demonstrate that our detected recruitment times are concordant with visually identified labels provided by an expert reviewer and are sufficiently accurate to model the spatiotemporal progression of seizure-associated traveling waves.

Conclusions: Our algorithm enables accurate cell recruitment detection and will serve as a useful tool for researchers investigating seizure dynamics using calcium imaging.

意义重大:显微镜下细胞钙成像已成为研究不同类型神经元如何在微电路水平相互作用以产生癫痫发作活动的有力工具,具有了解癫痫的新潜力。目的:展示利用钙成像检测癫痫发作相关事件的自动化算法框架--包括检测发作前尖峰事件、发作波阵面的传播以及群体级活动和单个细胞活动的终末扩散波:我们开发了一种在癫痫发作相关事件中精确检测群体和单个细胞招募的算法,该算法广泛利用平均群体活动和高幅度斜率特征来检测单细胞发作前尖峰和癫痫招募。我们将这种方法应用于戊四唑诱导的小鼠癫痫发作期间的清醒体内双光子钙成像记录数据:结果:我们证明了我们检测到的招募时间与专家评审员提供的视觉识别标签一致,并且足够准确,可以模拟癫痫发作相关行波的时空进展:我们的算法能够准确检测细胞招募,将成为研究人员利用钙成像研究癫痫发作动态的有用工具。
{"title":"Seizure event detection using intravital two-photon calcium imaging data.","authors":"Matthew A Stern, Eric R Cole, Robert E Gross, Ken Berglund","doi":"10.1117/1.NPh.11.2.024202","DOIUrl":"10.1117/1.NPh.11.2.024202","url":null,"abstract":"<p><strong>Significance: </strong>Intravital cellular calcium imaging has emerged as a powerful tool to investigate how different types of neurons interact at the microcircuit level to produce seizure activity, with newfound potential to understand epilepsy. Although many methods exist to measure seizure-related activity in traditional electrophysiology, few yet exist for calcium imaging.</p><p><strong>Aim: </strong>To demonstrate an automated algorithmic framework to detect seizure-related events using calcium imaging-including the detection of pre-ictal spike events, propagation of the seizure wavefront, and terminal spreading waves for both population-level activity and that of individual cells.</p><p><strong>Approach: </strong>We developed an algorithm for precise recruitment detection of population and individual cells during seizure-associated events, which broadly leverages averaged population activity and high-magnitude slope features to detect single-cell pre-ictal spike and seizure recruitment. We applied this method to data recorded using awake <i>in vivo</i> two-photon calcium imaging during pentylenetetrazol-induced seizures in mice.</p><p><strong>Results: </strong>We demonstrate that our detected recruitment times are concordant with visually identified labels provided by an expert reviewer and are sufficiently accurate to model the spatiotemporal progression of seizure-associated traveling waves.</p><p><strong>Conclusions: </strong>Our algorithm enables accurate cell recruitment detection and will serve as a useful tool for researchers investigating seizure dynamics using calcium imaging.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024202"},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10809036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139565435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering luminopsins with improved coupling efficiencies. 改进耦合效率的发光蛋白工程。
IF 5.3 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-03-29 DOI: 10.1117/1.NPh.11.2.024208
Ashley N Slaviero, Nipun Gorantla, Jacob Simkins, Emmanuel L Crespo, Ebenezer C Ikefuama, Maya O Tree, Mansi Prakash, Andreas Björefeldt, Lauren M Barnett, Gerard G Lambert, Diane Lipscombe, Christopher I Moore, Nathan C Shaner, Ute Hochgeschwender

Significance: Luminopsins (LMOs) are bioluminescent-optogenetic tools with a luciferase fused to an opsin that allow bimodal control of neurons by providing both optogenetic and chemogenetic access. Determining which design features contribute to the efficacy of LMOs will be beneficial for further improving LMOs for use in research.

Aim: We investigated the relative impact of luciferase brightness, opsin sensitivity, pairing of emission and absorption wavelength, and arrangement of moieties on the function of LMOs.

Approach: We quantified efficacy of LMOs through whole cell patch clamp recordings in HEK293 cells by determining coupling efficiency, the percentage of maximum LED induced photocurrent achieved with bioluminescent activation of an opsin. We confirmed key results by multielectrode array recordings in primary neurons.

Results: Luciferase brightness and opsin sensitivity had the most impact on the efficacy of LMOs, and N-terminal fusions of luciferases to opsins performed better than C-terminal and multi-terminal fusions. Precise paring of luciferase emission and opsin absorption spectra appeared to be less critical.

Conclusions: Whole cell patch clamp recordings allowed us to quantify the impact of different characteristics of LMOs on their function. Our results suggest that coupling brighter bioluminescent sources to more sensitive opsins will improve LMO function. As bioluminescent activation of opsins is most likely based on Förster resonance energy transfer, the most effective strategy for improving LMOs further will be molecular evolution of luciferase-fluorescent protein-opsin fusions.

意义重大:发光体蛋白(LMOs)是一种生物发光-光遗传工具,其荧光素酶与眼动蛋白相融合,可通过光遗传和化学遗传两种途径对神经元进行双模控制。目的:我们研究了荧光素酶亮度、光学视蛋白灵敏度、发射和吸收波长配对以及分子排列对 LMO 功能的相对影响:方法:我们通过在 HEK293 细胞中进行全细胞膜片钳记录来量化 LMOs 的功效,方法是确定耦合效率,即生物发光激活蛋白时 LED 诱导的最大光电流的百分比。我们通过在原代神经元中进行多电极阵列记录确认了主要结果:结果:荧光素酶亮度和光蛋白敏感性对 LMO 的功效影响最大,荧光素酶与光蛋白的 N 端融合优于 C 端和多端融合。荧光素酶发射光谱和蛋白吸收光谱的精确匹配似乎并不那么关键:全细胞膜片钳记录使我们能够量化 LMOs 不同特性对其功能的影响。我们的研究结果表明,将更亮的生物发光源与更灵敏的眼动蛋白耦合将改善 LMO 的功能。由于荧光蛋白的生物发光激活很可能是基于福斯特共振能量转移,因此进一步改进 LMO 的最有效策略将是荧光素酶-荧光蛋白-荧光蛋白融合的分子进化。
{"title":"Engineering luminopsins with improved coupling efficiencies.","authors":"Ashley N Slaviero, Nipun Gorantla, Jacob Simkins, Emmanuel L Crespo, Ebenezer C Ikefuama, Maya O Tree, Mansi Prakash, Andreas Björefeldt, Lauren M Barnett, Gerard G Lambert, Diane Lipscombe, Christopher I Moore, Nathan C Shaner, Ute Hochgeschwender","doi":"10.1117/1.NPh.11.2.024208","DOIUrl":"10.1117/1.NPh.11.2.024208","url":null,"abstract":"<p><strong>Significance: </strong>Luminopsins (LMOs) are bioluminescent-optogenetic tools with a luciferase fused to an opsin that allow bimodal control of neurons by providing both optogenetic and chemogenetic access. Determining which design features contribute to the efficacy of LMOs will be beneficial for further improving LMOs for use in research.</p><p><strong>Aim: </strong>We investigated the relative impact of luciferase brightness, opsin sensitivity, pairing of emission and absorption wavelength, and arrangement of moieties on the function of LMOs.</p><p><strong>Approach: </strong>We quantified efficacy of LMOs through whole cell patch clamp recordings in HEK293 cells by determining coupling efficiency, the percentage of maximum LED induced photocurrent achieved with bioluminescent activation of an opsin. We confirmed key results by multielectrode array recordings in primary neurons.</p><p><strong>Results: </strong>Luciferase brightness and opsin sensitivity had the most impact on the efficacy of LMOs, and N-terminal fusions of luciferases to opsins performed better than C-terminal and multi-terminal fusions. Precise paring of luciferase emission and opsin absorption spectra appeared to be less critical.</p><p><strong>Conclusions: </strong>Whole cell patch clamp recordings allowed us to quantify the impact of different characteristics of LMOs on their function. Our results suggest that coupling brighter bioluminescent sources to more sensitive opsins will improve LMO function. As bioluminescent activation of opsins is most likely based on Förster resonance energy transfer, the most effective strategy for improving LMOs further will be molecular evolution of luciferase-fluorescent protein-opsin fusions.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024208"},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10980360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140337688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human brain tissue identification using coherent anti-Stokes Raman scattering spectroscopy and diffuse reflectance spectroscopy for deep brain stimulation surgery. 利用相干反斯托克斯拉曼散射光谱学和漫反射光谱学识别人脑组织,用于脑深部刺激手术。
IF 5.3 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-06-12 DOI: 10.1117/1.NPh.11.2.025006
Sébastien Jerczynski, Mireille Quémener, Valérie Pineau Noël, Antoine Rousseau, Elahe Parham, Alexandre Bédard, Shadi Masoumi, Thomas Charland, Anthony Drouin, Jonathan Roussel, Valérie Dionne, Thomas Shooner, Anaïs Parrot, Mohamad A Takech, Éric Philippe, Damon DePaoli, Léo Cantin, Martin Parent, Daniel C Côté

Significance: We assess the feasibility of using diffuse reflectance spectroscopy (DRS) and coherent anti-Stokes Raman scattering spectroscopy (CARS) as optical tools for human brain tissue identification during deep brain stimulation (DBS) lead insertion, thereby providing a promising avenue for additional real-time neurosurgical guidance.

Aim: We developed a system that can acquire CARS and DRS spectra during the DBS surgery procedure to identify the tissue composition along the lead trajectory.

Approach: DRS and CARS spectra were acquired using a custom-built optical probe integrated in a commercial DBS lead. The lead was inserted to target three specific regions in each of the brain hemispheres of a human cadaver. Spectra were acquired during the lead insertion at constant position increments. Spectra were analyzed to classify each spectrum as being from white matter (WM) or gray matter (GM). The results were compared with tissue classification performed on histological brain sections.

Results: DRS and CARS spectra obtained using the optical probe can identify WM and GM during DBS lead insertion. The tissue composition along the trajectory toward a specific target is unique and can be differentiated by the optical probe. Moreover, the results obtained with principal component analysis suggest that DRS might be able to detect the presence of blood due to the strong optical absorption of hemoglobin.

Conclusions: It is possible to use optical measurements from the DBS lead during surgery to identify WM and GM and possibly the presence of blood in human brain tissue. The proposed optical tool could inform the surgeon during the lead placement if the lead has reached the target as planned. Our tool could eventually replace microelectrode recordings, which would streamline the process and reduce surgery time. Further developments are required to fully integrate these tools into standard clinical procedures.

意义重大:我们评估了在脑深部刺激(DBS)导联插入过程中使用漫反射光谱(DRS)和相干反斯托克斯拉曼散射光谱(CARS)作为光学工具识别人脑组织的可行性,从而为更多的实时神经外科指导提供了一条有希望的途径。目的:我们开发了一种系统,可以在 DBS 手术过程中获取 CARS 和 DRS 光谱,以识别导联轨迹上的组织成分:方法:使用集成在商用 DBS 导联中的定制光学探针获取 DRS 和 CARS 光谱。将导联线插入人体尸体大脑半球的三个特定区域。在导联插入过程中以恒定的位置增量采集光谱。对频谱进行分析,将每个频谱分为白质(WM)和灰质(GM)。结果与脑组织切片上进行的组织分类进行了比较:结果:使用光学探针获得的 DRS 和 CARS 光谱可以在 DBS 导联插入过程中识别 WM 和 GM。沿特定目标轨迹的组织成分是独一无二的,可通过光学探针加以区分。此外,通过主成分分析获得的结果表明,由于血红蛋白的强光学吸收,DRS 可能能够检测到血液的存在:结论:在手术过程中,利用 DBS 导联的光学测量结果来识别 WM 和 GM 以及人体脑组织中可能存在的血液是可行的。建议的光学工具可在导联放置过程中告知外科医生导联是否已按计划到达目标。我们的工具最终可以取代微电极记录,从而简化手术过程并缩短手术时间。要将这些工具完全整合到标准临床程序中,还需要进一步的开发。
{"title":"Human brain tissue identification using coherent anti-Stokes Raman scattering spectroscopy and diffuse reflectance spectroscopy for deep brain stimulation surgery.","authors":"Sébastien Jerczynski, Mireille Quémener, Valérie Pineau Noël, Antoine Rousseau, Elahe Parham, Alexandre Bédard, Shadi Masoumi, Thomas Charland, Anthony Drouin, Jonathan Roussel, Valérie Dionne, Thomas Shooner, Anaïs Parrot, Mohamad A Takech, Éric Philippe, Damon DePaoli, Léo Cantin, Martin Parent, Daniel C Côté","doi":"10.1117/1.NPh.11.2.025006","DOIUrl":"10.1117/1.NPh.11.2.025006","url":null,"abstract":"<p><strong>Significance: </strong>We assess the feasibility of using diffuse reflectance spectroscopy (DRS) and coherent anti-Stokes Raman scattering spectroscopy (CARS) as optical tools for human brain tissue identification during deep brain stimulation (DBS) lead insertion, thereby providing a promising avenue for additional real-time neurosurgical guidance.</p><p><strong>Aim: </strong>We developed a system that can acquire CARS and DRS spectra during the DBS surgery procedure to identify the tissue composition along the lead trajectory.</p><p><strong>Approach: </strong>DRS and CARS spectra were acquired using a custom-built optical probe integrated in a commercial DBS lead. The lead was inserted to target three specific regions in each of the brain hemispheres of a human cadaver. Spectra were acquired during the lead insertion at constant position increments. Spectra were analyzed to classify each spectrum as being from white matter (WM) or gray matter (GM). The results were compared with tissue classification performed on histological brain sections.</p><p><strong>Results: </strong>DRS and CARS spectra obtained using the optical probe can identify WM and GM during DBS lead insertion. The tissue composition along the trajectory toward a specific target is unique and can be differentiated by the optical probe. Moreover, the results obtained with principal component analysis suggest that DRS might be able to detect the presence of blood due to the strong optical absorption of hemoglobin.</p><p><strong>Conclusions: </strong>It is possible to use optical measurements from the DBS lead during surgery to identify WM and GM and possibly the presence of blood in human brain tissue. The proposed optical tool could inform the surgeon during the lead placement if the lead has reached the target as planned. Our tool could eventually replace microelectrode recordings, which would streamline the process and reduce surgery time. Further developments are required to fully integrate these tools into standard clinical procedures.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"025006"},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11167480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141312281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Parkinson's disease patients show delayed hemodynamic changes in primary motor cortex in fine motor tasks and decreased resting-state interhemispheric functional connectivity: a functional near-infrared spectroscopy study. 帕金森病患者在完成精细运动任务时初级运动皮层的血流动力学变化延迟,静息态半球间功能连通性降低:功能性近红外光谱研究。
IF 5.3 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-05-29 DOI: 10.1117/1.NPh.11.2.025004
Edgar Guevara, Francisco Javier Rivas-Ruvalcaba, Eleazar Samuel Kolosovas-Machuca, Miguel Ramírez-Elías, Ramón Díaz de León Zapata, Jose Luis Ramirez-GarciaLuna, Ildefonso Rodríguez-Leyva

Significance: People with Parkinson's disease (PD) experience changes in fine motor skills, which is viewed as one of the hallmark signs of this disease. Due to its non-invasive nature and portability, functional near-infrared spectroscopy (fNIRS) is a promising tool for assessing changes related to fine motor skills.

Aim: We aim to compare activation patterns in the primary motor cortex using fNIRS, comparing volunteers with PD and sex- and age-matched control participants during a fine motor task and walking. Moreover, inter and intrahemispheric functional connectivity (FC) was investigated during the resting state.

Approach: We used fNIRS to measure the hemodynamic changes in the primary motor cortex elicited by a finger-tapping task in 20 PD patients and 20 controls matched for age, sex, education, and body mass index. In addition, a two-minute walking task was carried out. Resting-state FC was also assessed.

Results: Patients with PD showed delayed hypoactivation in the motor cortex during the fine motor task with the dominant hand and delayed hyperactivation with the non-dominant hand. The findings also revealed significant correlations among various measures of hemodynamic activity in the motor cortex using fNIRS and different cognitive and clinical variables. There were no significant differences between patients with PD and controls during the walking task. However, there were significant differences in interhemispheric connectivity between PD patients and control participants, with a statistically significant decrease in PD patients compared with control participants.

Conclusions: Decreased interhemispheric FC and delayed activity in the primary motor cortex elicited by a fine motor task may one day serve as one of the many potential neuroimaging biomarkers for diagnosing PD.

意义重大:帕金森病(PD)患者的精细运动技能会发生变化,这被视为该疾病的标志性症状之一。由于其非侵入性和便携性,功能性近红外光谱(fNIRS)是评估精细运动技能相关变化的一种很有前途的工具。目的:我们旨在使用 fNIRS 比较初级运动皮层的激活模式,比较帕金森病志愿者与性别和年龄匹配的对照组参与者在精细运动任务和行走过程中的激活模式。此外,我们还研究了静息状态下大脑半球间和半球内的功能连接(FC):方法:我们使用 fNIRS 测量了 20 名帕金森病患者和 20 名年龄、性别、教育程度和体重指数相匹配的对照组患者在完成手指敲击任务时初级运动皮层的血流动力学变化。此外,还进行了两分钟步行任务。此外,还对静息态 FC 进行了评估:结果表明:在使用优势手完成精细运动任务时,帕金森病患者的运动皮层表现出延迟性激活不足,而使用非优势手时则表现出延迟性激活过度。研究结果还显示,使用 fNIRS 测量运动皮层的各种血流动力学活动与不同的认知和临床变量之间存在明显的相关性。在行走任务中,帕金森病患者与对照组之间没有明显差异。然而,帕金森氏症患者与对照组参与者的大脑半球间连通性存在明显差异,帕金森氏症患者的大脑半球间连通性与对照组参与者相比出现了统计学意义上的显著下降:由精细运动任务引起的半球间连通性降低和初级运动皮层活动延迟有朝一日可能会成为诊断帕金森病的众多潜在神经影像生物标志物之一。
{"title":"Parkinson's disease patients show delayed hemodynamic changes in primary motor cortex in fine motor tasks and decreased resting-state interhemispheric functional connectivity: a functional near-infrared spectroscopy study.","authors":"Edgar Guevara, Francisco Javier Rivas-Ruvalcaba, Eleazar Samuel Kolosovas-Machuca, Miguel Ramírez-Elías, Ramón Díaz de León Zapata, Jose Luis Ramirez-GarciaLuna, Ildefonso Rodríguez-Leyva","doi":"10.1117/1.NPh.11.2.025004","DOIUrl":"10.1117/1.NPh.11.2.025004","url":null,"abstract":"<p><strong>Significance: </strong>People with Parkinson's disease (PD) experience changes in fine motor skills, which is viewed as one of the hallmark signs of this disease. Due to its non-invasive nature and portability, functional near-infrared spectroscopy (fNIRS) is a promising tool for assessing changes related to fine motor skills.</p><p><strong>Aim: </strong>We aim to compare activation patterns in the primary motor cortex using fNIRS, comparing volunteers with PD and sex- and age-matched control participants during a fine motor task and walking. Moreover, inter and intrahemispheric functional connectivity (FC) was investigated during the resting state.</p><p><strong>Approach: </strong>We used fNIRS to measure the hemodynamic changes in the primary motor cortex elicited by a finger-tapping task in 20 PD patients and 20 controls matched for age, sex, education, and body mass index. In addition, a two-minute walking task was carried out. Resting-state FC was also assessed.</p><p><strong>Results: </strong>Patients with PD showed delayed hypoactivation in the motor cortex during the fine motor task with the dominant hand and delayed hyperactivation with the non-dominant hand. The findings also revealed significant correlations among various measures of hemodynamic activity in the motor cortex using fNIRS and different cognitive and clinical variables. There were no significant differences between patients with PD and controls during the walking task. However, there were significant differences in interhemispheric connectivity between PD patients and control participants, with a statistically significant decrease in PD patients compared with control participants.</p><p><strong>Conclusions: </strong>Decreased interhemispheric FC and delayed activity in the primary motor cortex elicited by a fine motor task may one day serve as one of the many potential neuroimaging biomarkers for diagnosing PD.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"025004"},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11135928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wavelength-swept spontaneous Raman spectroscopy system improves fiber-based collection efficiency for whole brain tissue classification. 波长扫描自发拉曼光谱系统提高了基于光纤的全脑组织分类收集效率。
IF 4.8 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-06-19 DOI: 10.1117/1.NPh.11.2.025007
Elahe Parham, Antoine Rousseau, Mireille Quémener, Martin Parent, Daniel C Côté

Significance: Raman spectroscopy is a valuable technique for tissue identification, but its conventional implementation is hindered by low efficiency due to scattering. Addressing this limitation, we are further developing the wavelength-swept Raman spectroscopy approach.

Aim: We aim to enhance Raman signal detection by employing a laser capable of sweeping over a wide wavelength range to sequentially excite tissue with different wavelengths, paired with a photodetector featuring a fixed narrow-bandpass filter for collecting the Raman signal at a specific wavelength.

Approach: We experimentally validate our technique using a fiber-based swept-source Raman spectroscopy setup. In addition, simulations are conducted to assess the efficacy of our approach in comparison with conventional spectrometer-based Raman spectroscopy.

Results: Our simulations reveal that the wavelength-swept configuration leads to a significantly stronger signal compared with conventional spectrometer-based Raman spectroscopy. Experimentally, our setup demonstrates an improvement of at least 200× in photon detection compared with the spectrometer-based setup. Furthermore, data acquired from different regions of a fixed monkey brain using our technique achieves 99% accuracy in classification via k -nearest neighbor analysis.

Conclusions: Our study showcases the potential of wavelength-swept Raman spectroscopy for tissue identification, particularly in highly scattering media, such as the brain. The developed technique offers enhanced signal detection capabilities, paving the way for future in vivo applications in tissue characterization.

意义重大:拉曼光谱是一种用于组织鉴定的重要技术,但由于散射导致的低效率,阻碍了拉曼光谱的传统应用。针对这一局限,我们正在进一步开发波长扫描拉曼光谱方法。目的:我们旨在通过使用能够在宽波长范围内扫描的激光器,以不同波长依次激发组织,并搭配具有固定窄带通滤波器的光电探测器,以收集特定波长的拉曼信号,从而增强拉曼信号检测:方法:我们使用基于光纤的扫源拉曼光谱装置对我们的技术进行了实验验证。此外,我们还进行了模拟,以评估我们的方法与传统光谱仪拉曼光谱法的功效:我们的模拟结果表明,与传统的基于光谱仪的拉曼光谱相比,波长扫频配置能产生更强的信号。实验证明,与基于光谱仪的装置相比,我们的装置在光子探测方面至少提高了 200 倍。此外,利用我们的技术从固定猴脑的不同区域获取的数据,通过 k 近邻分析进行分类的准确率达到 99%:我们的研究展示了波长扫描拉曼光谱在组织识别方面的潜力,尤其是在大脑等高散射介质中。所开发的技术具有更强的信号检测能力,为未来组织表征的体内应用铺平了道路。
{"title":"Wavelength-swept spontaneous Raman spectroscopy system improves fiber-based collection efficiency for whole brain tissue classification.","authors":"Elahe Parham, Antoine Rousseau, Mireille Quémener, Martin Parent, Daniel C Côté","doi":"10.1117/1.NPh.11.2.025007","DOIUrl":"10.1117/1.NPh.11.2.025007","url":null,"abstract":"<p><strong>Significance: </strong>Raman spectroscopy is a valuable technique for tissue identification, but its conventional implementation is hindered by low efficiency due to scattering. Addressing this limitation, we are further developing the wavelength-swept Raman spectroscopy approach.</p><p><strong>Aim: </strong>We aim to enhance Raman signal detection by employing a laser capable of sweeping over a wide wavelength range to sequentially excite tissue with different wavelengths, paired with a photodetector featuring a fixed narrow-bandpass filter for collecting the Raman signal at a specific wavelength.</p><p><strong>Approach: </strong>We experimentally validate our technique using a fiber-based swept-source Raman spectroscopy setup. In addition, simulations are conducted to assess the efficacy of our approach in comparison with conventional spectrometer-based Raman spectroscopy.</p><p><strong>Results: </strong>Our simulations reveal that the wavelength-swept configuration leads to a significantly stronger signal compared with conventional spectrometer-based Raman spectroscopy. Experimentally, our setup demonstrates an improvement of at least 200× in photon detection compared with the spectrometer-based setup. Furthermore, data acquired from different regions of a fixed monkey brain using our technique achieves 99% accuracy in classification via <math><mrow><mi>k</mi></mrow> </math> -nearest neighbor analysis.</p><p><strong>Conclusions: </strong>Our study showcases the potential of wavelength-swept Raman spectroscopy for tissue identification, particularly in highly scattering media, such as the brain. The developed technique offers enhanced signal detection capabilities, paving the way for future <i>in vivo</i> applications in tissue characterization.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"025007"},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141428242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Special Section Guest Editorial: Molecular Neurophotonics. 特约编辑:分子神经光子学。
IF 4.8 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-06-28 DOI: 10.1117/1.NPh.11.2.024200
Ute Hochgeschwender, Robert E Campbell, Hideaki Mizuno

The editorial introduces the Special Section on Molecular Neurophotonics.

社论介绍了 "分子神经光子学 "专栏。
{"title":"Special Section Guest Editorial: Molecular Neurophotonics.","authors":"Ute Hochgeschwender, Robert E Campbell, Hideaki Mizuno","doi":"10.1117/1.NPh.11.2.024200","DOIUrl":"https://doi.org/10.1117/1.NPh.11.2.024200","url":null,"abstract":"<p><p>The editorial introduces the Special Section on Molecular Neurophotonics.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024200"},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11213545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Three-dimensional infrared scanning: an enhanced approach for spatial registration of probes for neuroimaging. 三维红外扫描:神经成像探针空间配准的增强方法。
IF 4.8 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-04-01 Epub Date: 2024-05-26 DOI: 10.1117/1.NPh.11.2.024309
András Bálint, Christian Rummel, Marco Caversaccio, Stefan Weder

Significance: Accurate spatial registration of probes (e.g., optodes and electrodes) for measurement of brain activity is a crucial aspect in many neuroimaging modalities. It may increase measurement precision and enable the transition from channel-based calculations to volumetric representations.

Aim: This technical note evaluates the efficacy of a commercially available infrared three-dimensional (3D) scanner under actual experimental (or clinical) conditions and provides guidelines for its use.

Method: We registered probe positions using an infrared 3D scanner and validated them against magnetic resonance imaging (MRI) scans on five volunteer participants.

Results: Our analysis showed that with standard cap fixation, the average Euclidean distance of probe position among subjects could reach up to 43 mm, with an average distance of 15.25 mm [standard deviation (SD) = 8.0]. By contrast, the average distance between the infrared 3D scanner and the MRI-acquired positions was 5.69 mm (SD = 1.73), while the average difference between consecutive infrared 3D scans was 3.43 mm (SD = 1.62). The inter-optode distance, which was fixed at 30 mm, was measured as 29.28 mm (SD = 1.12) on the MRI and 29.43 mm (SD = 1.96) on infrared 3D scans. Our results demonstrate the high accuracy and reproducibility of the proposed spatial registration method, making it suitable for both functional near-infrared spectroscopy and electroencephalogram studies.

Conclusions: The 3D infrared scanning technique for spatial registration of probes provides economic efficiency, simplicity, practicality, repeatability, and high accuracy, with potential benefits for a range of neuroimaging applications. We provide practical guidance on anonymization, labeling, and post-processing of acquired scans.

意义重大:在许多神经成像模式中,对用于测量大脑活动的探头(如光电二极管和电极)进行准确的空间配准是一个至关重要的方面。目的:本技术说明评估了市售红外三维(3D)扫描仪在实际实验(或临床)条件下的功效,并提供了使用指南:我们使用红外三维扫描仪记录探头位置,并与五名志愿参与者的磁共振成像(MRI)扫描结果进行对比验证:我们的分析表明,在标准帽固定的情况下,受试者探头位置的平均欧氏距离可达 43 毫米,平均距离为 15.25 毫米[标准差 (SD) = 8.0]。相比之下,红外三维扫描仪与核磁共振成像获取位置之间的平均距离为 5.69 毫米(标准差 = 1.73),而连续红外三维扫描之间的平均差异为 3.43 毫米(标准差 = 1.62)。光栅间距固定为 30 毫米,核磁共振成像测量结果为 29.28 毫米(标准差 = 1.12),红外三维扫描结果为 29.43 毫米(标准差 = 1.96)。我们的研究结果表明,所提出的空间配准方法具有很高的准确性和可重复性,因此适用于功能性近红外光谱仪和脑电图研究:用于探针空间配准的三维红外扫描技术具有经济高效、简单实用、可重复性强和准确度高等优点,可用于一系列神经成像应用。我们为匿名化、标记和后处理获取的扫描结果提供了实用指导。
{"title":"Three-dimensional infrared scanning: an enhanced approach for spatial registration of probes for neuroimaging.","authors":"András Bálint, Christian Rummel, Marco Caversaccio, Stefan Weder","doi":"10.1117/1.NPh.11.2.024309","DOIUrl":"10.1117/1.NPh.11.2.024309","url":null,"abstract":"<p><strong>Significance: </strong>Accurate spatial registration of probes (e.g., optodes and electrodes) for measurement of brain activity is a crucial aspect in many neuroimaging modalities. It may increase measurement precision and enable the transition from channel-based calculations to volumetric representations.</p><p><strong>Aim: </strong>This technical note evaluates the efficacy of a commercially available infrared three-dimensional (3D) scanner under actual experimental (or clinical) conditions and provides guidelines for its use.</p><p><strong>Method: </strong>We registered probe positions using an infrared 3D scanner and validated them against magnetic resonance imaging (MRI) scans on five volunteer participants.</p><p><strong>Results: </strong>Our analysis showed that with standard cap fixation, the average Euclidean distance of probe position among subjects could reach up to 43 mm, with an average distance of 15.25 mm [standard deviation (SD) = 8.0]. By contrast, the average distance between the infrared 3D scanner and the MRI-acquired positions was 5.69 mm (SD = 1.73), while the average difference between consecutive infrared 3D scans was 3.43 mm (SD = 1.62). The inter-optode distance, which was fixed at 30 mm, was measured as 29.28 mm (SD = 1.12) on the MRI and 29.43 mm (SD = 1.96) on infrared 3D scans. Our results demonstrate the high accuracy and reproducibility of the proposed spatial registration method, making it suitable for both functional near-infrared spectroscopy and electroencephalogram studies.</p><p><strong>Conclusions: </strong>The 3D infrared scanning technique for spatial registration of probes provides economic efficiency, simplicity, practicality, repeatability, and high accuracy, with potential benefits for a range of neuroimaging applications. We provide practical guidance on anonymization, labeling, and post-processing of acquired scans.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024309"},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11134420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Neurophotonics
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1