Resampling the same satellite image to conduct a multi-scale assessment of desertification can be accompanied by distortion of terrestrial objects and spectral information, which can lead to uncertainty in the generated information. To address this, this study assesses desertification severity in an area of arid and semi-arid climate in the Eastern Mediterranean (Jordan) that is characterised by cloudless scenes using multi-sensor data of the same scene at the same time. To this end, Sentinel-2 at 10 m and 60 m, Landsat-8 at 30 m and MODIS at 250 m and 500 m were collected to extract albedo and modified soil adjusted vegetation index (MSAVI), and subsequently to construct albedo-MSAVI feature space. Using the negative correlation between albedo and MSAVI, desertification degree index (DDI) was generated. The resulting multi-scale DDI maps bear a relative resemblance in terms of spatial distribution, patterns, and proportions. The DDI maps indicate that extremely serious and serious desertification are widespread, accounting for 50% of the study area, primarily in the eastern portions. However, finer DDI maps (10 m, 30 m and 60 m) are essential for detecting small-scale desertification characteristics due to their ability to capture local spatial variabilities, while coarser ones (250 m and 500 m) are better suited for capturing broad-scale desertification patterns driven by climatic factors, in which MODIS data exhibit a relatively higher positive correlation with seasonal average precipitation. Although finer DDI maps show higher accuracy compared to coarser ones, the accuracy of DDI maps of MODIS has shown an increase within a homogeneous landscape. Accordingly, synchronised multi-scale assessment of desertification severity is not only influenced by the spatial resolution but also by the landscape heterogeneity and the type of satellite sensor utilised. The multi-scale approach applied in this study can provide insights on scale-dependent desertification that help in devising overarching mitigation strategies.