N. Beusker, J. Müller, F. Seehusen, A. Cehak, W. Baumgärtner, K. Feige
{"title":"Histiocytic sarcoma in a 16-year-old pony gelding.","authors":"N. Beusker, J. Müller, F. Seehusen, A. Cehak, W. Baumgärtner, K. Feige","doi":"10.21836/PEM20100502","DOIUrl":"https://doi.org/10.21836/PEM20100502","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"34 1","pages":"680-684"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68457300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Rechenberg, S. Tanner, M. Hilbe, K. Zlinszky, Käthi Kämpf, F. Wedel, J. Kümmerle, S. Picek, R. Vogt, J. Auer
Periosteal transection and elevation is a standard treatment for angular limb deformities in foals. It is used to correct axis deviations in the limbs at an early age to assure that the foals grow up with straight limbs to improve their chances to reach their full potential as future athletes. Although clinically proven, its mechanisms of action were never elucidated on a more basic scientific level. In this experimental study the molecular response to periosteal stripping was investigated within the growth plate and adjacent perichondrium. The study was based on the hypothesis that a growth restraining feedback loop related to Indian hedgehog (Ihh), parathyroid hormone related protein (PTHrP) and parathyroid hormone receptors (PTHR) was responsible for the corrective effect of periosteal stripping. Twelve 3 months old lambs underwent periosteal stripping of the distal lateral radius and tibia on one side. The contralateral side served as non-operated controls. Two animals each group were sacrificed at 2, 6, 10, 14, 18 and 21 days after surgery and the growth plates with minimal adjacent bone tissue were harvested for histological investigations. After decalcification, paraffin-embedded sections with routine hematoxylin-eosin stains were prepared to assess morphology and length of growth plates, whereas immunohistochemistry of Ihh, PTHrP, PTHR and the two cytokines fibroblast(FGF) and transforming growth factor (TGF) was performed to study different protein expression between operated limbs and controls. The results indicate that periosteal stripping caused an up-regulation of Ihh in the early preand hypertrophic zone of the growth plate, followed by an increase of PTHrP mainly in the perichondrium, while an increase of PTHR was noticed in all zones, although highest in the perichondrium and hypertrophic zones. The growth factors FGF and TGF were upregulated in all zones, but FGF in response to periosteal stripping was more intensely expressed in the proliferative zone and the highest peak of TGF was found in the perichondrium. Length measurements of the various growth zones revealed significant negative correlations between the proliferative and pre-and hypertrophic zones, indicating that indeed a negative feed back loop after periosteal stripping exists coupled by the Ihh/PTHrP/PTHR cascade.The hypothesis that periosteal stripping had an effect on the Ihh/PTHrP/PTHR related feedback loop in epiphyseal growth was confirmed in this experimental study in lambs. Since these mechanisms are very basic and similar in most species, it can be safely assumed that the effects in foals are similar. In fact, the asymmetric mechanical load in animals suffering from axis deviation may even increase the enhancing effect of length correction.
{"title":"Molecular signaling within growth plates of the radius and tibia after periosteal stripping: an experimental study in lambs","authors":"B. Rechenberg, S. Tanner, M. Hilbe, K. Zlinszky, Käthi Kämpf, F. Wedel, J. Kümmerle, S. Picek, R. Vogt, J. Auer","doi":"10.21836/PEM20100401","DOIUrl":"https://doi.org/10.21836/PEM20100401","url":null,"abstract":"Periosteal transection and elevation is a standard treatment for angular limb deformities in foals. It is used to correct axis deviations in the limbs at an early age to assure that the foals grow up with straight limbs to improve their chances to reach their full potential as future athletes. Although clinically proven, its mechanisms of action were never elucidated on a more basic scientific level. In this experimental study the molecular response to periosteal stripping was investigated within the growth plate and adjacent perichondrium. The study was based on the hypothesis that a growth restraining feedback loop related to Indian hedgehog (Ihh), parathyroid hormone related protein (PTHrP) and parathyroid hormone receptors (PTHR) was responsible for the corrective effect of periosteal stripping. Twelve 3 months old lambs underwent periosteal stripping of the distal lateral radius and tibia on one side. The contralateral side served as non-operated controls. Two animals each group were sacrificed at 2, 6, 10, 14, 18 and 21 days after surgery and the growth plates with minimal adjacent bone tissue were harvested for histological investigations. After decalcification, paraffin-embedded sections with routine hematoxylin-eosin stains were prepared to assess morphology and length of growth plates, whereas immunohistochemistry of Ihh, PTHrP, PTHR and the two cytokines fibroblast(FGF) and transforming growth factor (TGF) was performed to study different protein expression between operated limbs and controls. The results indicate that periosteal stripping caused an up-regulation of Ihh in the early preand hypertrophic zone of the growth plate, followed by an increase of PTHrP mainly in the perichondrium, while an increase of PTHR was noticed in all zones, although highest in the perichondrium and hypertrophic zones. The growth factors FGF and TGF were upregulated in all zones, but FGF in response to periosteal stripping was more intensely expressed in the proliferative zone and the highest peak of TGF was found in the perichondrium. Length measurements of the various growth zones revealed significant negative correlations between the proliferative and pre-and hypertrophic zones, indicating that indeed a negative feed back loop after periosteal stripping exists coupled by the Ihh/PTHrP/PTHR cascade.The hypothesis that periosteal stripping had an effect on the Ihh/PTHrP/PTHR related feedback loop in epiphyseal growth was confirmed in this experimental study in lambs. Since these mechanisms are very basic and similar in most species, it can be safely assumed that the effects in foals are similar. In fact, the asymmetric mechanical load in animals suffering from axis deviation may even increase the enhancing effect of length correction.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"26 1","pages":"484-494"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68456570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A retrospective study was carried out to investigate the prevalence and causes of laminitis affecting limbs other than the contralateral healthy limb after orthopedic surgery in horses. Of 3,500 horses referred for orthopedic surgery at the Equine Clinic, Vetsuisse-Faculty, University of Zurich, between 1992 and 2007, eight horses that developed laminitis in one or more limbs, but not primarily the contralateral limb, were identified. The reasons for referral were synovial space infection (n = 4), pastern joint arthrodesis (n = 1), fracture of the central tarsal bone (n = 1), bilateral stifle osteochondrosis (n = 1) and luxation of the superficial digital flexor tendon (n = 1). Postoperative complications included infection of the surgery site (n = 1), severe postoperative pain (n = 2), postoperative myopathy (n = 1), urticaria (n = 1) and cecal impaction (n = 1). The administration of analgesics was discontinued eight days after surgery because of urticaria in one horse and one day after surgery because of cecal impaction in another horse. Seven of the eight horses were euthanized because of severe laminitis. Elimination of infection and effective postoperative analgesia appear important in the prevention of laminitis. Severe postoperative pain should be prevented with adequate, continuous and multimodal pain management. It should also be noted that laminitis may occur in response to excessive weight bearing in limbs other than the contralateral limb after orthopedic surgery.
{"title":"Atypical laminitis after orthopedic surgery in eight horses","authors":"A. Widmer, M. Kummer, R. Bettschart, A. Fürst","doi":"10.21836/PEM20090606","DOIUrl":"https://doi.org/10.21836/PEM20090606","url":null,"abstract":"A retrospective study was carried out to investigate the prevalence and causes of laminitis affecting limbs other than the contralateral healthy \u0000limb after orthopedic surgery in horses. Of 3,500 horses referred for orthopedic surgery at the Equine Clinic, Vetsuisse-Faculty, University \u0000of Zurich, between 1992 and 2007, eight horses that developed laminitis in one or more limbs, but not primarily the contralateral \u0000limb, were identified. The reasons for referral were synovial space infection (n = 4), pastern joint arthrodesis (n = 1), fracture of the central \u0000tarsal bone (n = 1), bilateral stifle osteochondrosis (n = 1) and luxation of the superficial digital flexor tendon (n = 1). Postoperative \u0000complications included infection of the surgery site (n = 1), severe postoperative pain (n = 2), postoperative myopathy (n = 1), urticaria \u0000(n = 1) and cecal impaction (n = 1). The administration of analgesics was discontinued eight days after surgery because of urticaria in \u0000one horse and one day after surgery because of cecal impaction in another horse. Seven of the eight horses were euthanized because of \u0000severe laminitis. Elimination of infection and effective postoperative analgesia appear important in the prevention of laminitis. Severe postoperative \u0000pain should be prevented with adequate, continuous and multimodal pain management. It should also be noted that laminitis \u0000may occur in response to excessive weight bearing in limbs other than the contralateral limb after orthopedic surgery.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"576-580"},"PeriodicalIF":0.3,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68456294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
auch von langen Röhrenknochen erfolgreich operiert werden. The most important first aid measure in an orthopaedic emergency is the stabilisation of the fractured limb in the correct position so that it bears some weight but not enough to damage soft tissue structures. Manipulation of the limb is usually painful and necessitates sedation and the use of analgesics. Reducing or eliminating fear and pain in the patient prevents complications. In addition to analgesics, antibio-tics should be given and the horse transported to an appropriate clinic. Recent advances in equine orthopaedic surgery have allowed com-plicated fractures to be successfully repaired.
auch von langen Röhrenknochen erfolgreich operiert werden。在骨科紧急情况下，最重要的急救措施是将骨折肢体稳定在正确的位置，使其承受一定的重量，但不至于损伤软组织结构。肢体的操作通常是痛苦的，需要镇静和使用镇痛剂。减少或消除病人的恐惧和痛苦可以预防并发症。除了止痛剂外，还应给予抗生素，并将马送到适当的诊所。马骨科手术的最新进展使复杂的骨折得以成功修复。
{"title":"Notfallversorgung und Transport des Frakturpferdes","authors":"Anton Fürst, M. Jackson, R. Keller, B. V. Salis","doi":"10.5167/UZH-31026","DOIUrl":"https://doi.org/10.5167/UZH-31026","url":null,"abstract":"auch von langen Röhrenknochen erfolgreich operiert werden. The most important first aid measure in an orthopaedic emergency is the stabilisation of the fractured limb in the correct position so that it bears some weight but not enough to damage soft tissue structures. Manipulation of the limb is usually painful and necessitates sedation and the use of analgesics. Reducing or eliminating fear and pain in the patient prevents complications. In addition to analgesics, antibio-tics should be given and the horse transported to an appropriate clinic. Recent advances in equine orthopaedic surgery have allowed com-plicated fractures to be successfully repaired.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"1-7"},"PeriodicalIF":0.3,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70648366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Laus, F. Mariotti, G. Magi, D. Spaziante, M. Fugazzola, B. Tesei
Summary The aim of this paper is to describe the clinical, histopathological and immunohistochemical findings of a mammary carcinoma in a 22-year-old, 10 month pregnant, thoroughbred mare. The clinical examination of the right mammary gland showed a firm enlargement and purulent discharge from the ulcerated skin surface and the teat. During necropsy visceral metastases were not found but inguinal lymph nodes presented metastatic infiltrations when observed histologically. None of the previous reports found positive immunohisthochemical reactions for estrogen and progesteron receptors in an equine mammary adenocarcinoma. However, the findings of positive immunoreactivity to steroid receptors could suggest a hormonal influence on the genesis of the mammary neoplasm, as demonstrated in human breast cancer. der neoplastischen Epithelzellen festgestellt. In dem noch gesundem Gesäugegewebe gab es eine positive Reaktion auf Östrogenrezeptoren und auf Progesteronrezeptoren. Dieser erste Fall eines positiven immunhistochemischen Steroid-hormonrezeptornachweises beim Pferd könnte für einen hormonellen Einfluss bei der Genese der Mammatumoren sprechen, wie es bei Mammatumoren des Menschen bekannt ist.
本文的目的是描述22岁，怀孕10个月的纯种母马乳腺癌的临床，组织病理学和免疫组织化学表现。右乳腺的临床检查显示坚实的扩大和脓性分泌物从溃疡的皮肤表面和乳头。尸检未发现内脏转移，但组织学观察发现腹股沟淋巴结有转移性浸润。以前的报道没有发现马乳腺腺癌中雌激素和孕激素受体的阳性免疫组化反应。然而，类固醇受体阳性免疫反应的发现可能表明激素对乳腺肿瘤发生的影响，正如在人类乳腺癌中所证明的那样。der瘤变上皮细胞。在他们的研究中，gesundem Gesäugegewebe和孕激素受体(progesteronreeptoren)的反应均为阳性。dieer erste Fall eines阳性免疫组织化学(immunohistochemistry)甾体激素(steroids - hormone - rezeptornachweises beem Pferd) könnte freinen hormone Einfluss bei der Genese der Mammatumoren prerechen, es bei Mammatumoren des Menschen bekant ist。
{"title":"Mammary carcinoma in a mare: clinical, histopathological and steroid hormone receptor status.","authors":"F. Laus, F. Mariotti, G. Magi, D. Spaziante, M. Fugazzola, B. Tesei","doi":"10.21836/PEM20090103","DOIUrl":"https://doi.org/10.21836/PEM20090103","url":null,"abstract":"Summary The aim of this paper is to describe the clinical, histopathological and immunohistochemical findings of a mammary carcinoma in a 22-year-old, 10 month pregnant, thoroughbred mare. The clinical examination of the right mammary gland showed a firm enlargement and purulent discharge from the ulcerated skin surface and the teat. During necropsy visceral metastases were not found but inguinal lymph nodes presented metastatic infiltrations when observed histologically. None of the previous reports found positive immunohisthochemical reactions for estrogen and progesteron receptors in an equine mammary adenocarcinoma. However, the findings of positive immunoreactivity to steroid receptors could suggest a hormonal influence on the genesis of the mammary neoplasm, as demonstrated in human breast cancer. der neoplastischen Epithelzellen festgestellt. In dem noch gesundem Gesäugegewebe gab es eine positive Reaktion auf Östrogenrezeptoren und auf Progesteronrezeptoren. Dieser erste Fall eines positiven immunhistochemischen Steroid-hormonrezeptornachweises beim Pferd könnte für einen hormonellen Einfluss bei der Genese der Mammatumoren sprechen, wie es bei Mammatumoren des Menschen bekannt ist.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"18-21"},"PeriodicalIF":0.3,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68454682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clinical anatomy of the splanchnocranium region of the equine using magnetic resonance imaging.","authors":"K. Gerlach, K. Flatz, W. Brehm, J. Seeger","doi":"10.21836/PEM20090107","DOIUrl":"https://doi.org/10.21836/PEM20090107","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"45-53"},"PeriodicalIF":0.3,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68455411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Hoffmann, F. Bazer, J. Klug, H. Aupperle, C. Ellenberger, S. Özgen, D. Schoon, H. Schoon
Little is known about the stage of estrus cycle-dependent variations of uterine secretions of the more and most published results were based on evaluation of uterine flushings. Hence, the aim of this study was to define patterns of expression of different endometrial proteins throughout the estrus cycle using newly established immunohistochemical methods and endometrial biopsies. Therefore, endometrial biopsies were collected from three mares on defined days of the estrus cycle with known concentrations of estradiol and progesterone in serum. The proteins uteroglobin, uteroferrin, calbindin(D9k) and uterocalin were examined using polyclonal antibodies. Furthermore glycogen was identified using the PAS-reaction prior to and after a-amylase digestion. During the estrus cycle expression of the examined proteins, as well as glycogen, revealed a typical, progesterone-dependent pattern. In the early- and mid-interestrus there is a simultaneous increase in expression of uterocalin and glycogen staining intensity associated with increasing concentrations of progesterone in peripheral blood. The maximal expression of calbindin(D9k) was also in association with high levels of circulating progesterone in mid-interestrus. In contrast, secretion of both uteroglobin and uteroferrin was simultaneously associated with the decrease in circulating concentrations of progesterone in mid-to late-interestrus. However, it has to be emphasized that distinct among mare differences were obvious. This new method enables pathologists to interpret changes in the uterine secretion pattern associated with fertility reducing diseases by using a single endometrial biopsy
{"title":"Immunohistochemical and histochemical identification of proteins and carbohydrates in the equine endometrium: reaction patterns in the cycling mare.","authors":"C. Hoffmann, F. Bazer, J. Klug, H. Aupperle, C. Ellenberger, S. Özgen, D. Schoon, H. Schoon","doi":"10.21836/PEM20090303","DOIUrl":"https://doi.org/10.21836/PEM20090303","url":null,"abstract":"Little is known about the stage of estrus cycle-dependent variations of uterine secretions of the more and most published results were based on evaluation of uterine flushings. Hence, the aim of this study was to define patterns of expression of different endometrial proteins throughout the estrus cycle using newly established immunohistochemical methods and endometrial biopsies. Therefore, endometrial biopsies were collected from three mares on defined days of the estrus cycle with known concentrations of estradiol and progesterone in serum. The proteins uteroglobin, uteroferrin, calbindin(D9k) and uterocalin were examined using polyclonal antibodies. Furthermore glycogen was identified using the PAS-reaction prior to and after a-amylase digestion. During the estrus cycle expression of the examined proteins, as well as glycogen, revealed a typical, progesterone-dependent pattern. In the early- and mid-interestrus there is a simultaneous increase in expression of uterocalin and glycogen staining intensity associated with increasing concentrations of progesterone in peripheral blood. The maximal expression of calbindin(D9k) was also in association with high levels of circulating progesterone in mid-interestrus. In contrast, secretion of both uteroglobin and uteroferrin was simultaneously associated with the decrease in circulating concentrations of progesterone in mid-to late-interestrus. However, it has to be emphasized that distinct among mare differences were obvious. This new method enables pathologists to interpret changes in the uterine secretion pattern associated with fertility reducing diseases by using a single endometrial biopsy","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"212-219"},"PeriodicalIF":0.3,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68456155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tissue Doppler echocardiographic examinations at rest and after exercise in horses with atrial fibrillation.","authors":"H. Gehlen, C. Iversen, P. Stadler","doi":"10.21836/PEM20090102","DOIUrl":"https://doi.org/10.21836/PEM20090102","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"11-16"},"PeriodicalIF":0.3,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68454560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tissue Doppler Imaging in the horse.","authors":"H. Gehlen, C. Iversen, P. Stadler","doi":"10.21836/pem20090101","DOIUrl":"https://doi.org/10.21836/pem20090101","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"4-10"},"PeriodicalIF":0.3,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68454547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence of EHV-2 and EHV-5 DNA in ocular and nasal swabs as well as peripheral blood mononuclear cells.","authors":"N. Richter, M. Ebert, K. Borchers","doi":"10.21836/PEM20090106","DOIUrl":"https://doi.org/10.21836/PEM20090106","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"38-44"},"PeriodicalIF":0.3,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68455323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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