S. Picek, K. Kalchofner, S. Ringer, M. Kummer, A. Fürst, R. Bettschart‐Wolfensberger
Summary This study retrospectively describes the anaesthetic management before and during hydropool recovery in horses. Fifty horses (mean age 113.7 ± 64.2 months, mean weight 479.3 ± 115.3 kg) underwent surgery for repair of different long bone fractures and other severe orthopaedic diseases. Thirty-nine horses were starved for 8-12 hours and eleven horses were presented as emergency cases, which needed immediate intervention. Intravenous antibiotics, acepromazine (0.03 mg/kg IM) and phenylbutazone (4 mg/kg IV) were administered 30-60 minutes before anaesthesia induction. Sedation was achieved using medetomidine (7 mcg/kg IV) and anaesthesia induction with 2.2 mg/kg ketamine and 0.02 mg/kg diazepam IV. Anaesthesia was maintained with isoflurane (in oxygen and air) and medetomidine constant rate infusion (3.5 mcg/kg/h). Horses breathed spontaneously during the whole procedure. Mean arterial blood pressure was maintained between 70 and 90 mmHg with dobutamine (< 1.25 mcg/kg/min) and lactated ringer’s solution (5-10 ml/kg/h). All horses were administered 6-10 ml/kg hetastarch (10%). For recovery, horses were lifted with a rescue sling into a hydropool (37°C), the head was supported by an inflatable cushion. In order to increase the quality of recovery the horses were given 0.1 mg/kg morphine IM, 2 mcg/kg medetomidine IV and oxygen (15 l/min) intratracheally in the hydropool. Mean duration of anaesthesia was 251.4 ± 90.2 (97-540) minutes with no major anaesthetic complications. Mean duration of horses’ recovery to a standing position outside the pool was 63 ± 20 (40-140) minutes. For one horse (withers height 185 cm) the pool was not deep enough and the horse became excited during recovery in the hydropool. During assisted recovery in a conventional recovery box it refractured its radius and was euthanized. All other horses successfully recovered. Management as described provided excellent conditions.
{"title":"Anaesthetic management for hydropool recovery in 50 horses","authors":"S. Picek, K. Kalchofner, S. Ringer, M. Kummer, A. Fürst, R. Bettschart‐Wolfensberger","doi":"10.21836/PEM20100404","DOIUrl":"https://doi.org/10.21836/PEM20100404","url":null,"abstract":"Summary This study retrospectively describes the anaesthetic management before and during hydropool recovery in horses. Fifty horses (mean age 113.7 ± 64.2 months, mean weight 479.3 ± 115.3 kg) underwent surgery for repair of different long bone fractures and other severe orthopaedic diseases. Thirty-nine horses were starved for 8-12 hours and eleven horses were presented as emergency cases, which needed immediate intervention. Intravenous antibiotics, acepromazine (0.03 mg/kg IM) and phenylbutazone (4 mg/kg IV) were administered 30-60 minutes before anaesthesia induction. Sedation was achieved using medetomidine (7 mcg/kg IV) and anaesthesia induction with 2.2 mg/kg ketamine and 0.02 mg/kg diazepam IV. Anaesthesia was maintained with isoflurane (in oxygen and air) and medetomidine constant rate infusion (3.5 mcg/kg/h). Horses breathed spontaneously during the whole procedure. Mean arterial blood pressure was maintained between 70 and 90 mmHg with dobutamine (< 1.25 mcg/kg/min) and lactated ringer’s solution (5-10 ml/kg/h). All horses were administered 6-10 ml/kg hetastarch (10%). For recovery, horses were lifted with a rescue sling into a hydropool (37°C), the head was supported by an inflatable cushion. In order to increase the quality of recovery the horses were given 0.1 mg/kg morphine IM, 2 mcg/kg medetomidine IV and oxygen (15 l/min) intratracheally in the hydropool. Mean duration of anaesthesia was 251.4 ± 90.2 (97-540) minutes with no major anaesthetic complications. Mean duration of horses’ recovery to a standing position outside the pool was 63 ± 20 (40-140) minutes. For one horse (withers height 185 cm) the pool was not deep enough and the horse became excited during recovery in the hydropool. During assisted recovery in a conventional recovery box it refractured its radius and was euthanized. All other horses successfully recovered. Management as described provided excellent conditions.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"25 1","pages":"515-522"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68456931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Niemendal, L. Hoelzle, K. Rohn, K. Feige, T. Blaha
{"title":"Studies on the clinical relevance of microscopically demonstrable eperythrozoon-like structures on horse-erythrocytes.","authors":"R. Niemendal, L. Hoelzle, K. Rohn, K. Feige, T. Blaha","doi":"10.21836/PEM20100504","DOIUrl":"https://doi.org/10.21836/PEM20100504","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"26 1","pages":"691-696"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68457367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Niemendal, L. Hoelzle, K. Rahn, K. Feige, T. Blaha
Hamotrophe Mykoplasmen (Haemobartonella und Eperythrozoon Spezies) sind weitverbreitete Bakterien, die auf roten Blutzellen parasitieren. Sie sind Erreger der Haemobartonellose und der Eperythrozoonose. Infektionen mit hamotrophen Mykoplasmen sind beim Schwein, beim Schaf, bei der Ziege, beim Rind, bei der Katze, beim Hund und bei der Maus ausfuhrlich dokumentiert. In der aktuellen wissenschaftlichen Literatur sind diese Infektionen beim Pferd nicht beschrieben. Seit einigen Jahren kommt es aber immer haufiger zu Berichten aus der Praxis uber den mikroskopischen Nachweis einer vermuteten Eperythrozoonose bei Pferden. In der vorliegenden Studie wurden Blutproben von 108 Pferden, darunter 76 Tiere (Gruppe K) mit ungestortem Allgemeinbefinden und 32 Pferde mit unspezifischen klinischen Symptomen in Form von Asthenie, schlechtem Fellzustand, Abmagerung und Leistungsschwache (Gruppe S) zytologisch und hamatologisch untersucht. Drei Untersucher beurteilten Blutausstriche unabhangig voneinander hinsichtlich des Vorkommens von Eperythrozoon-ahnlichen Strukturen auf den Erythrozyten. Als Eperythrozoon-ahnliche Strukturen wurden punktformige, dunkelviolett gefarbte meist multipel und in der Peripherie der Erythrozyten auftretende Veranderungen definiert. Zudem wurde eine hamatologische Untersuchung mit der Bestimmung von Leukozyten, Erythrozyten, Hamoglobin, Hamatokrit, MCH, MCHC, MCV und Thrombozyten durchgefuhrt. Eperythrozoon-ahnliche Strukturen wurden sehr haufig bei Tieren mit Symptomen (27, 22 und 7 positive zytologische Beurteilungen), als auch bei allgemein gesunden Pferden (55, 50 und 14 positive zytologische Beurteilungen), festgestellt. Signifikante Unterschiede zwischen beiden Untersuchungsgruppen konnten weder bei den zytologischen noch bei den hamatologischen Untersuchungen festgestellt werden. Die Ubereinstimmung in der Beurteilung der drei Untersucher war sehr gering (κ-Werte 0,0225 und -0,0148). Es wurden keine Hinweise auf das vermehrte Vorkommen von Anamien bei symptomatischen Tieren (Gruppe S) gefunden. Auf Grund der Erkenntnisse uber hamotrophe Mykoplasmen bei verschiedenen Tierarten und der in der vorliegenden Arbeit erzielten Ergebnisse kann bei Pferden, bei denen in der zytologischen Untersuchung verdachtige Strukturen auf den Erythrozyten nachgewiesen wurden, nicht ohne Weiteres von einer Infektion mit hamotrophen Mykoplasmen ausgegangen werden. Erst wenn hamotrophe Mykoplasmen nach phylogenetischer Genanalyse beim Pferd beschrieben und ein spezifischer PCR-Test entwickelt ist, kann die Diagnose einer Infektion mit diesem Erreger beim Pferd mit Sicherheit gestellt werden.
{"title":"Untersuchungen zur klinischen Relevanz mikroskopisch nachweisbarer Eperythrozoon-ähnlicher Strukturen auf Pferde-Erythrozyten","authors":"R. Niemendal, L. Hoelzle, K. Rahn, K. Feige, T. Blaha","doi":"10.5167/UZH-35694","DOIUrl":"https://doi.org/10.5167/UZH-35694","url":null,"abstract":"Hamotrophe Mykoplasmen (Haemobartonella und Eperythrozoon Spezies) sind weitverbreitete Bakterien, die auf roten Blutzellen parasitieren. Sie sind Erreger der Haemobartonellose und der Eperythrozoonose. Infektionen mit hamotrophen Mykoplasmen sind beim Schwein, beim Schaf, bei der Ziege, beim Rind, bei der Katze, beim Hund und bei der Maus ausfuhrlich dokumentiert. In der aktuellen wissenschaftlichen Literatur sind diese Infektionen beim Pferd nicht beschrieben. Seit einigen Jahren kommt es aber immer haufiger zu Berichten aus der Praxis uber den mikroskopischen Nachweis einer vermuteten Eperythrozoonose bei Pferden. In der vorliegenden Studie wurden Blutproben von 108 Pferden, darunter 76 Tiere (Gruppe K) mit ungestortem Allgemeinbefinden und 32 Pferde mit unspezifischen klinischen Symptomen in Form von Asthenie, schlechtem Fellzustand, Abmagerung und Leistungsschwache (Gruppe S) zytologisch und hamatologisch untersucht. Drei Untersucher beurteilten Blutausstriche unabhangig voneinander hinsichtlich des Vorkommens von Eperythrozoon-ahnlichen Strukturen auf den Erythrozyten. Als Eperythrozoon-ahnliche Strukturen wurden punktformige, dunkelviolett gefarbte meist multipel und in der Peripherie der Erythrozyten auftretende Veranderungen definiert. Zudem wurde eine hamatologische Untersuchung mit der Bestimmung von Leukozyten, Erythrozyten, Hamoglobin, Hamatokrit, MCH, MCHC, MCV und Thrombozyten durchgefuhrt. Eperythrozoon-ahnliche Strukturen wurden sehr haufig bei Tieren mit Symptomen (27, 22 und 7 positive zytologische Beurteilungen), als auch bei allgemein gesunden Pferden (55, 50 und 14 positive zytologische Beurteilungen), festgestellt. Signifikante Unterschiede zwischen beiden Untersuchungsgruppen konnten weder bei den zytologischen noch bei den hamatologischen Untersuchungen festgestellt werden. Die Ubereinstimmung in der Beurteilung der drei Untersucher war sehr gering (κ-Werte 0,0225 und -0,0148). Es wurden keine Hinweise auf das vermehrte Vorkommen von Anamien bei symptomatischen Tieren (Gruppe S) gefunden. Auf Grund der Erkenntnisse uber hamotrophe Mykoplasmen bei verschiedenen Tierarten und der in der vorliegenden Arbeit erzielten Ergebnisse kann bei Pferden, bei denen in der zytologischen Untersuchung verdachtige Strukturen auf den Erythrozyten nachgewiesen wurden, nicht ohne Weiteres von einer Infektion mit hamotrophen Mykoplasmen ausgegangen werden. Erst wenn hamotrophe Mykoplasmen nach phylogenetischer Genanalyse beim Pferd beschrieben und ein spezifischer PCR-Test entwickelt ist, kann die Diagnose einer Infektion mit diesem Erreger beim Pferd mit Sicherheit gestellt werden.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"26 1","pages":"691-696"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70649963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anton Fürst, M. Jackson, Jan M Kümmerle, Regula Bettschart-Wolfensberger, M. Kummer
head on a hard surface during their fall. Injuries of the head range from small lesions, which may only be apparent as small indentations in the bone, to severe, multifragmented fractures with secondary involvement of vital structures such as teeth, sinuses, eyes, nerves or major blood vessels. The extent and type of these secondary lesions are usually the most important prognostic factors. Therefore, the description and understanding of head fractures requires a good working knowledge of the anatomy of the skull. The clinical signs of head fractures are manifold and depend on the location of the lesions. Computed tomography (CT) is a very useful diagnostic procedure that is often indicated for the assessment of complicated head injuries. It had the greatest impact in the management of diseases involving the head, particularly fractures, for horses. They are frequently more dramatic on CT images than on radiographs or as compared to what one would expect after external inspection. Nowadays most head fractures are treated surgically and generally the prognosis is very good. The majority of rostral fractures can be treated successfully with intraoral wiring. For fractures of the skull and sinuses a variety of special instruments for the repositioning and fixation of skull bone fragments have been developed in human medicine. Several of them, including extraction instruments and rosettes, are useful in equine surgery.
{"title":"Übersicht über die aktuellen Therapiemöglichkeiten der Frakturen am Kopf des Pferdes","authors":"Anton Fürst, M. Jackson, Jan M Kümmerle, Regula Bettschart-Wolfensberger, M. Kummer","doi":"10.5167/UZH-42245","DOIUrl":"https://doi.org/10.5167/UZH-42245","url":null,"abstract":"head on a hard surface during their fall. Injuries of the head range from small lesions, which may only be apparent as small indentations in the bone, to severe, multifragmented fractures with secondary involvement of vital structures such as teeth, sinuses, eyes, nerves or major blood vessels. The extent and type of these secondary lesions are usually the most important prognostic factors. Therefore, the description and understanding of head fractures requires a good working knowledge of the anatomy of the skull. The clinical signs of head fractures are manifold and depend on the location of the lesions. Computed tomography (CT) is a very useful diagnostic procedure that is often indicated for the assessment of complicated head injuries. It had the greatest impact in the management of diseases involving the head, particularly fractures, for horses. They are frequently more dramatic on CT images than on radiographs or as compared to what one would expect after external inspection. Nowadays most head fractures are treated surgically and generally the prognosis is very good. The majority of rostral fractures can be treated successfully with intraoral wiring. For fractures of the skull and sinuses a variety of special instruments for the repositioning and fixation of skull bone fragments have been developed in human medicine. Several of them, including extraction instruments and rosettes, are useful in equine surgery.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"26 1","pages":"503-514"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70652749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To evaluate the outcome of pars plana vitrectomy (PPV) in eyes with clinical signs of equine recurrent uveitis (ERU) testing positive or negative for intravitreal antibodies against various serovars of Leptospira interrogans. 47 eyes of horses with ERU in which intraoperative vitreous samples were tested by microagglutination (MAT) for various serovars of Leptospira interrogans between 2001 and 2009 were included in a retrospective study. The presence or absence of postoperative ERU was evaluated by repeated ocular examinations, as well as by interrogation of referring veterinarians and owners. The absence of recurrent episodes of active uveitis was considered a success irrespective of the visual performance of the operated eyes. 85% of eyes of horses with ERU had antibodies against L. interrogans in their vitreous. The most common serovar was L. grippothyphosa. The majority of horses testing positive for antibodies against L. interrogans (40/47; 82.5%) showed no further episodes of ERU, while 6/7 (85.7%) of horses testing negative continued to experience episodes of ERU. This difference is statistically significant. It appears that PPV is an effective treatment for Leptospira-associated ERU but not for eyes testing negative for antibodies against L. interrogans. Vitreal and aqueous humor samples of horses suffering from ERU should be tested by MAT before performing a PPV. Keywords: Equine recurrent uveitis, vitrectomy, Leptospira interrogans, antibodies, ophthalmology
{"title":"The outcome of pars plana vitrectomy in horses with equine recurrent uveitis with regard to the presence or absence of intravitreal antibodies against various serovars of Leptospira interrogans.","authors":"E. Tömördy, M. Hässig, B. Spiess","doi":"10.21836/PEM20100222","DOIUrl":"https://doi.org/10.21836/PEM20100222","url":null,"abstract":"Purpose: To evaluate the outcome of pars plana vitrectomy (PPV) in eyes with clinical signs of equine recurrent uveitis (ERU) testing positive or negative for intravitreal antibodies against various serovars of Leptospira interrogans. 47 eyes of horses with ERU in which intraoperative vitreous samples were tested by microagglutination (MAT) for various serovars of Leptospira interrogans between 2001 and 2009 were included in a retrospective study. The presence or absence of postoperative ERU was evaluated by repeated ocular examinations, as well as by interrogation of referring veterinarians and owners. The absence of recurrent episodes of active uveitis was considered a success irrespective of the visual performance of the operated eyes. 85% of eyes of horses with ERU had antibodies against L. interrogans in their vitreous. The most common serovar was L. grippothyphosa. The majority of horses testing positive for antibodies against L. interrogans (40/47; 82.5%) showed no further episodes of ERU, while 6/7 (85.7%) of horses testing negative continued to experience episodes of ERU. This difference is statistically significant. It appears that PPV is an effective treatment for Leptospira-associated ERU but not for eyes testing negative for antibodies against L. interrogans. Vitreal and aqueous humor samples of horses suffering from ERU should be tested by MAT before performing a PPV. Keywords: Equine recurrent uveitis, vitrectomy, Leptospira interrogans, antibodies, ophthalmology","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"26 1","pages":"251-254"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68456509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Pongratz, Patrick R. Kircher, J. Láng, M. Hilbe, M. Eser
{"title":"Diagnosestellung bei einem Fohlen mit zerebellärer Abiotrophie mittels Magnetresonanztomographie (MRT)","authors":"M. Pongratz, Patrick R. Kircher, J. Láng, M. Hilbe, M. Eser","doi":"10.21836/pem20100410","DOIUrl":"https://doi.org/10.21836/pem20100410","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68457252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. S. Bland, M. T. Bayliss, B. Rechenberg, D. E. Ashhurst
{"title":"Typ 5-Kollagen im Gelenkknorpel von Mensch und Pferd","authors":"Y. S. Bland, M. T. Bayliss, B. Rechenberg, D. E. Ashhurst","doi":"10.21836/pem20100414","DOIUrl":"https://doi.org/10.21836/pem20100414","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68457383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. M. Melo, G. A. Monteiro, B. R. Avanzi, P. N. Guasti, M. Alvarenga, J. A. Dell'aqua, F. S. Zahn, F. Papa
Unexpected death, catastrophic injury, castration or any other event that makes both semen collection and mating impossible can be costly in terms of the potential loss of genetically valuable stallion genes. In such circumstances, owners may request a final semen collection to allow propagation of valuable germplasm (Cary et. al. 2004). There are limited data regarding survival and fertility of frozen-thawed epididymal stallion sperm, although the first pregnancy using frozen-thawed stallion spermatozoa was reported in a mare inseminated with epididymal spermatozoa (Barker and Gandier 1957). Stallion sperm capable of fertilization can be harvested from the cauda epididymis (Barker and Gandier 1957, Johnson et al. 1980) and stored for future use, preserving valuable gene pool. The timing and, often, location for either castration or post-mortem tissue recovery are not always appropriate for sperm handling and preservation.
意外死亡、灾难性伤害、阉割或任何其他导致精液收集和交配无法进行的事件，都可能导致具有遗传价值的种马基因的潜在损失，代价高昂。在这种情况下，所有者可能要求最后收集精液，以便繁殖有价值的种质(Cary等人，2004年)。关于冷冻解冻的种马附睾精子的存活率和生育能力的数据有限，尽管第一次使用冷冻解冻的种马精子怀孕是在一匹用附睾精子受精的母马中报道的(Barker和Gandier 1957)。能够受精的种马精子可以从附睾尾部收集(Barker and Gandier 1957, Johnson et al. 1980)并储存以备将来使用，保存有价值的基因库。阉割或死后组织恢复的时间和地点往往不适合处理和保存精子。
{"title":"Advances in stallion's epididymal sperm technology","authors":"C. M. Melo, G. A. Monteiro, B. R. Avanzi, P. N. Guasti, M. Alvarenga, J. A. Dell'aqua, F. S. Zahn, F. Papa","doi":"10.21836/PEM20100110","DOIUrl":"https://doi.org/10.21836/PEM20100110","url":null,"abstract":"Unexpected death, catastrophic injury, castration or any other event that makes both semen collection and mating impossible can be costly in terms of the potential loss of genetically valuable stallion genes. In such circumstances, owners may request a final semen collection to allow propagation of valuable germplasm (Cary et. al. 2004). There are limited data regarding survival and fertility of frozen-thawed epididymal stallion sperm, although the first pregnancy using frozen-thawed stallion spermatozoa was reported in a mare inseminated with epididymal spermatozoa (Barker and Gandier 1957). Stallion sperm capable of fertilization can be harvested from the cauda epididymis (Barker and Gandier 1957, Johnson et al. 1980) and stored for future use, preserving valuable gene pool. The timing and, often, location for either castration or post-mortem tissue recovery are not always appropriate for sperm handling and preservation.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"26 1","pages":"48-52"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68456497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Liesegang, R. Giezendanner, S. Tanner, B. Rechenberg, J. Auer
In the present study the effects of „periosteal stripping“ on bone and cartilage metabolism of foals with angular limb deformities and healthy experimental lambs were examined. Five foals with angular limb deformities were treated surgically by means of „hemicircumferential transection of the periosteum and periosteal stripping“ (HCTP/HP). Before surgery as well as twice after surgery, 1,25(OH)2Vitamin D, bone and cartilage markers in the serum were determined and compared to 5 healthy foals of the same age. In addition 10 healthy lambs were treated by HCTP/HP, in which the same parameters and bone density were determined. At 22 days after surgery the lambs were sacrificed and treated and control bones were sampled and processed for immunohistochemistry. Neither in foals nor in lambs, significant differences could be observed between treated and untreated animals with regard to the parameters measured in serum. Nevertheless, both treated groups revealed a tendency for higher bone remodelling compared to control animals. This could be the result of an increased bone remodelling rate following surgery. No statistically significant difference in bone mineral density between treated and untreated animals could be detected. In contrast local changes of signal transduction related to parathyroid hormone related protein and indian hedgehog (PTHrP/Ihh) were observed using immunhistochemistry. It was concluded that HCTP/HP at the level of epiphysis and metaphysis does not lead to systemic effects, even though local events detected by immunohistochemistry point to an increased bone formation at the treated site.
{"title":"Systemic and local effects of disportional longitudinal growth of bones in foals and lambs and the impact on bone mineral density and content","authors":"A. Liesegang, R. Giezendanner, S. Tanner, B. Rechenberg, J. Auer","doi":"10.21836/PEM20100402","DOIUrl":"https://doi.org/10.21836/PEM20100402","url":null,"abstract":"In the present study the effects of „periosteal stripping“ on bone and cartilage metabolism of foals with angular limb deformities and healthy experimental lambs were examined. Five foals with angular limb deformities were treated surgically by means of „hemicircumferential transection of the periosteum and periosteal stripping“ (HCTP/HP). Before surgery as well as twice after surgery, 1,25(OH)2Vitamin D, bone and cartilage markers in the serum were determined and compared to 5 healthy foals of the same age. In addition 10 healthy lambs were treated by HCTP/HP, in which the same parameters and bone density were determined. At 22 days after surgery the lambs were sacrificed and treated and control bones were sampled and processed for immunohistochemistry. Neither in foals nor in lambs, significant differences could be observed between treated and untreated animals with regard to the parameters measured in serum. Nevertheless, both treated groups revealed a tendency for higher bone remodelling compared to control animals. This could be the result of an increased bone remodelling rate following surgery. No statistically significant difference in bone mineral density between treated and untreated animals could be detected. In contrast local changes of signal transduction related to parathyroid hormone related protein and indian hedgehog (PTHrP/Ihh) were observed using immunhistochemistry. It was concluded that HCTP/HP at the level of epiphysis and metaphysis does not lead to systemic effects, even though local events detected by immunohistochemistry point to an increased bone formation at the treated site.","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"26 1","pages":"495-502"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68456865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Liver diseases in horses.","authors":"H. Gehlen, A. May, M. Venner","doi":"10.21836/PEM20100501","DOIUrl":"https://doi.org/10.21836/PEM20100501","url":null,"abstract":"","PeriodicalId":54626,"journal":{"name":"Pferdeheilkunde","volume":"26 1","pages":"668-679"},"PeriodicalIF":0.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68457458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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