Ocular Surface最新文献_第10页

Topical low-dose heparin in ocular Stevens Johnson Syndrome and associated molecular correlations: A randomized controlled pilot study 局部低剂量肝素治疗眼部史蒂文斯·约翰逊综合征及其相关分子相关性：一项随机对照先导研究。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-28 DOI: 10.1016/j.jtos.2025.02.013

Renu Venugopal , Shivam Sharma , Lata Singh , Seema Sen , Thirumurthy Velpandian , Prafulla Kumar Maharana , Seema Kashyap , Sandeep Jain , Namrata Sharma

Purpose

To elucidate the efficacy of topical low dose heparin (LDH) as an adjuvant therapy in of sub -chronic and chronic ocular Stevens-Johnson Syndrome (SJS) and its effect on Neutrophil extracellular traps (NETs) associated markers.

Design

A prospective randomized controlled pilot trial.

Methods

72 clinically diagnosed SJS cases were recruited into sub-chronic and chronic groups. Both groups were randomly given either LDH eye drops with conservative treatment (CT) or CT alone for one month. Visual acuity, Schirmer's test, fluorescein and lissamine staining, OSDI score and ocular surface severity were evaluated. Tear and conjunctival cells were collected to detect NET-MPO complexes and NET-gene markers (TNFSF14, TLR9, IL-6, MyD88, C3a gene).

Main outcome measures

Improvement in ocular surface severity scores.

Results

Sub-chronic group showed significant improvement with topical LDH in TBUT (p = 0.0001), Lissamine stain score (p = 0.0162), corneal vascularisation score (p = 0.0001), conjunctival hyperaemia score (p = 0.001) and total severity score (p = 0.001) when compared to the LDH treated chronic group. No significant improvement was seen in visual acuity in either group after one month of therapy. Qualitative ELISA confirmed higher presence of NET-MPO complex in sub-chronic cases. Both groups showed upregulation of TNFSF14, TLR9, and MyD88, which significantly decreased post topical LDH treatment (p = 0.0038,0.002 and 0.043 respectively).

Conclusion

The presence of NETs mediated upregulated immune markers on sub-chronic and chronic SJS cases sheds newer light on its unresolved ocular pathology. Subsequent administration of topical LDH revealed a notable mitigation of NET associated immune markers, highlighting its promising anti-inflammatory effects within the ocular microenvironment.

目的：探讨外用低剂量肝素（LDH）辅助治疗亚慢性和慢性眼史蒂文斯-约翰逊综合征（SJS）的疗效及其对中性粒细胞胞外陷阱（NETs）相关标志物的影响。设计：前瞻性随机对照试验方法：将72例临床诊断为SJS的患者分为亚慢性组和慢性组。两组随机给予LDH滴眼液加保守治疗（CT）或单独CT治疗一个月。评估视力、Schirmer试验、荧光素和丽丝胺染色、OSDI评分和眼表严重程度。收集泪液和结膜细胞检测NET-MPO复合物和net -基因标记物（TNFSF14、TLR9、IL-6、MyD88、C3a基因）。结果：与LDH治疗的慢性组相比，亚慢性组在TBUT （p=0.0001）、Lissamine染色评分（p= 0.0162）、角膜血管化评分（p=0.0001）、结膜充血评分（p= 0.001）和总严重性评分（p= 0.001）方面均有显著改善。治疗一个月后，两组患者的视力均未见明显改善。定性ELISA证实，亚慢性病例中NET-MPO复合物的存在较高。两组均表现出TNFSF14、TLR9和MyD88的上调，在局部LDH治疗后显著降低（p分别为0.0038、0.002和0.043）。结论：NETs介导的免疫标记上调在亚慢性和慢性SJS患者中的存在，为其未解决的眼部病理提供了新的思路。随后给予局部LDH显示出NET相关免疫标记物的显著缓解，突出了其在眼微环境中有希望的抗炎作用。

{"title":"Topical low-dose heparin in ocular Stevens Johnson Syndrome and associated molecular correlations: A randomized controlled pilot study","authors":"Renu Venugopal , Shivam Sharma , Lata Singh , Seema Sen , Thirumurthy Velpandian , Prafulla Kumar Maharana , Seema Kashyap , Sandeep Jain , Namrata Sharma","doi":"10.1016/j.jtos.2025.02.013","DOIUrl":"10.1016/j.jtos.2025.02.013","url":null,"abstract":"<div><h3>Purpose</h3><div>To elucidate the efficacy of topical low dose heparin (LDH) as an adjuvant therapy in of sub -chronic and chronic ocular Stevens-Johnson Syndrome (SJS) and its effect on Neutrophil extracellular traps (NETs) associated markers.</div></div><div><h3>Design</h3><div>A prospective randomized controlled pilot trial.</div></div><div><h3>Methods</h3><div>72 clinically diagnosed SJS cases were recruited into sub-chronic and chronic groups. Both groups were randomly given either LDH eye drops with conservative treatment (CT) or CT alone for one month. Visual acuity, Schirmer's test, fluorescein and lissamine staining, OSDI score and ocular surface severity were evaluated. Tear and conjunctival cells were collected to detect NET-MPO complexes and NET-gene markers (TNFSF14, TLR9, IL-6, MyD88, C3a gene).</div></div><div><h3>Main outcome measures</h3><div>Improvement in ocular surface severity scores.</div></div><div><h3>Results</h3><div>Sub-chronic group showed significant improvement with topical LDH in TBUT (p = 0.0001), Lissamine stain score (p = 0.0162), corneal vascularisation score (p = 0.0001), conjunctival hyperaemia score (p = 0.001) and total severity score (p = 0.001) when compared to the LDH treated chronic group. No significant improvement was seen in visual acuity in either group after one month of therapy. Qualitative ELISA confirmed higher presence of NET-MPO complex in sub-chronic cases. Both groups showed upregulation of TNFSF14, TLR9, and MyD88, which significantly decreased post topical LDH treatment (p = 0.0038,0.002 and 0.043 respectively).</div></div><div><h3>Conclusion</h3><div>The presence of NETs mediated upregulated immune markers on sub-chronic and chronic SJS cases sheds newer light on its unresolved ocular pathology. Subsequent administration of topical LDH revealed a notable mitigation of NET associated immune markers, highlighting its promising anti-inflammatory effects within the ocular microenvironment.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"37 ","pages":"Pages 47-56"},"PeriodicalIF":5.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the corneal immune microenvironment in healthy and diabetic mice during corneal wound healing 解码健康小鼠和糖尿病小鼠角膜伤口愈合过程中的角膜免疫微环境。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-27 DOI: 10.1016/j.jtos.2025.02.010

Yujing Lin , Xiaowen Zhang , Di Sun , Qun Wang , Shengqian Dou , Qingjun Zhou

Diabetic keratopathy (DK) is an underdiagnosed ocular complication of diabetes mellitus. The changes of ocular immune microenvironment contribute to the pathogenesis of DK, while precise mechanisms remain inadequately understood. Here, we employed single-cell RNA sequencing (scRNA-seq) to elucidate the transcriptional alterations of immune cells from diabetic and healthy control mouse corneas during homeostasis and wound healing. Unbiased clustering analysis unveiled 3 major cell subsets and 11 subdivided cell clusters, including T cells, monocyte lineages, and neutrophil subpopulations. The further sub-clustering analysis demonstrated that T cells exhibited cytotoxicity characteristics in both homeostasis and wound healing of diabetic cornea. Moreover, dendritic cells preferred the migratory and maturation phenotype and may recruit and maintain cytotoxic T cells. Macrophages in diabetic cornea preferred the pro-inflammatory M1 phenotype. Under injury conditions, diabetic corneal neutrophils exhibited a more mature and functional possession of neutrophil extracellular traps (NETs). Furthermore, cell-cell communication revealed that the immune cells exhibited hyperactivation and pro-inflammatory responses, while the monocyte lineages exhibited the activating effect on T cells in diabetic cornea. This study represents the inaugural effort to establish a comprehensive scRNA-Seq transcriptomic profile of corneal immune cells during wound healing in healthy and diabetic mice, which offers a valuable reference for subsequent investigations into the pathological roles of immune cells in DK.

糖尿病性角膜病变（DK）是糖尿病的一种未被诊断的眼部并发症。眼部免疫微环境的改变参与了DK的发病机制，但其具体机制尚不清楚。在这里，我们使用单细胞RNA测序（scRNA-seq）来阐明来自糖尿病小鼠和健康对照小鼠角膜的免疫细胞在体内平衡和伤口愈合过程中的转录改变。无偏聚类分析揭示了3个主要的细胞亚群和11个细分的细胞群，包括T细胞、单核细胞谱系和中性粒细胞亚群。进一步的亚聚类分析表明，T细胞在糖尿病角膜的稳态和伤口愈合中都表现出细胞毒性特征。此外，树突状细胞倾向于迁移和成熟表型，并可能招募和维持细胞毒性T细胞。糖尿病角膜中的巨噬细胞倾向于促炎性M1表型。在损伤条件下，糖尿病角膜中性粒细胞表现出更成熟和功能性的中性粒细胞细胞外陷阱（NETs）。此外，细胞间通讯显示，免疫细胞表现出过度活化和促炎反应，而单核细胞谱系则表现出对糖尿病角膜T细胞的激活作用。本研究首次建立了健康和糖尿病小鼠创面愈合过程中角膜免疫细胞的scRNA-Seq转录组图谱，为后续研究免疫细胞在DK中的病理作用提供了有价值的参考。

{"title":"Decoding the corneal immune microenvironment in healthy and diabetic mice during corneal wound healing","authors":"Yujing Lin , Xiaowen Zhang , Di Sun , Qun Wang , Shengqian Dou , Qingjun Zhou","doi":"10.1016/j.jtos.2025.02.010","DOIUrl":"10.1016/j.jtos.2025.02.010","url":null,"abstract":"<div><div>Diabetic keratopathy (DK) is an underdiagnosed ocular complication of diabetes mellitus. The changes of ocular immune microenvironment contribute to the pathogenesis of DK, while precise mechanisms remain inadequately understood. Here, we employed single-cell RNA sequencing (scRNA-seq) to elucidate the transcriptional alterations of immune cells from diabetic and healthy control mouse corneas during homeostasis and wound healing. Unbiased clustering analysis unveiled 3 major cell subsets and 11 subdivided cell clusters, including T cells, monocyte lineages, and neutrophil subpopulations. The further sub-clustering analysis demonstrated that T cells exhibited cytotoxicity characteristics in both homeostasis and wound healing of diabetic cornea. Moreover, dendritic cells preferred the migratory and maturation phenotype and may recruit and maintain cytotoxic T cells. Macrophages in diabetic cornea preferred the pro-inflammatory M1 phenotype. Under injury conditions, diabetic corneal neutrophils exhibited a more mature and functional possession of neutrophil extracellular traps (NETs). Furthermore, cell-cell communication revealed that the immune cells exhibited hyperactivation and pro-inflammatory responses, while the monocyte lineages exhibited the activating effect on T cells in diabetic cornea. This study represents the inaugural effort to establish a comprehensive scRNA-Seq transcriptomic profile of corneal immune cells during wound healing in healthy and diabetic mice, which offers a valuable reference for subsequent investigations into the pathological roles of immune cells in DK.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"37 ","pages":"Pages 68-79"},"PeriodicalIF":5.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A prospective, longitudinal study to assess progression of ocular surface signs, tear cytokines and protein profiles in young adults 一项前瞻性的纵向研究，以评估年轻人眼表体征，泪液细胞因子和蛋白质谱的进展。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-25 DOI: 10.1016/j.jtos.2025.02.011

Rachel K. Casemore , James S. Wolffsohn , Tor Paaske Utheim , Sjur Reppe , Hans Christian D. Aass , Debarun Dutta

Purpose

To compare ocular surface characteristics, tear protein profiles, and cytokines in young adults with and without evaporative dry eye disease (DED), exploring any associations with lifestyle factors, and determine any progression after one year.

Methods

Fifty participants, aged 18–25 years, were recruited. Detailed ocular surface parameters were assessed following administration of lifestyle and symptom questionnaires. Tear samples collected by microcapillary tubes were analysed using the Agilent Bioanalyzer (7 proteins between 14 and 230 kDa); tears collected with Schirmer strips were analysed for ten cytokines using Luminex Assay.

Results

56 % of participants fulfilled the TFOS DEWS II criteria for DED. 48 % had at least 25 % meibomian gland loss in either lid regardless of dry eye status, while over 90 % had at least one diagnostic sign. Progression was observed, characterised by significant increases (p < 0.05) in ocular redness, lid wiper epitheliopathy and blink rate. Albumin was upregulated (p = 0.003) in DED, while zinc-α2-glycoprotein, which showed significant correlations with several meibomian gland parameters, was downregulated. Upregulation of both pro- and anti-inflammatory cytokines was observed, with several significant clinical correlations, including IL-1β with meibomian gland parameters.

Conclusions

Evidence of inflammation and overlap of ocular signs in these young adults reinforces the need for early detection and differentiation of those likely to progress to DED. While upregulation of both pro- and anti-inflammatory cytokines has provided evidence of a mechanism to maintain homeostasis, the subtle progression of ocular surface disease observed suggests that counselling is required around the modifiable risk factors of DED identified, regardless of whether symptoms are present or not.

目的：比较患有和未患有蒸发性干眼症（DED）的年轻人的眼表特征、泪液蛋白图谱和细胞因子，探讨与生活方式因素的关联，并确定一年后的病情发展情况：方法：招募了 50 名 18-25 岁的参与者。方法：招募了 50 名 18-25 岁的参与者，在发放生活方式和症状调查问卷后，对他们的眼表参数进行了详细评估。使用微毛细管采集的泪液样本用安捷伦生物分析仪进行分析（7 种蛋白质的含量在 14-230 kDa 之间）；使用施尔默试纸采集的泪液样本用 Luminex 分析仪分析 10 种细胞因子：56% 的参与者符合 TFOS DEWS II 的 DED 标准。48%的受试者无论是否患有干眼症，两侧眼睑均有至少25%的睑板腺脱落，而90%以上的受试者至少有一种诊断征兆。研究发现，干眼症患者的眼睑睑板腺脱落程度在不断加深，其特征是眼睑睑板腺脱落程度显著增加（pConclusions）：在这些年轻人中，炎症和眼部体征重叠的证据加强了早期发现和区分可能发展为 DED 的人群的必要性。虽然促炎和抗炎细胞因子的上调提供了维持平衡机制的证据，但观察到的眼表疾病的微妙进展表明，无论是否出现症状，都需要围绕已确定的 DED 可改变风险因素进行咨询。

{"title":"A prospective, longitudinal study to assess progression of ocular surface signs, tear cytokines and protein profiles in young adults","authors":"Rachel K. Casemore , James S. Wolffsohn , Tor Paaske Utheim , Sjur Reppe , Hans Christian D. Aass , Debarun Dutta","doi":"10.1016/j.jtos.2025.02.011","DOIUrl":"10.1016/j.jtos.2025.02.011","url":null,"abstract":"<div><h3>Purpose</h3><div>To compare ocular surface characteristics, tear protein profiles, and cytokines in young adults with and without evaporative dry eye disease (DED), exploring any associations with lifestyle factors, and determine any progression after one year.</div></div><div><h3>Methods</h3><div>Fifty participants, aged 18–25 years, were recruited. Detailed ocular surface parameters were assessed following administration of lifestyle and symptom questionnaires. Tear samples collected by microcapillary tubes were analysed using the Agilent Bioanalyzer (7 proteins between 14 and 230 kDa); tears collected with Schirmer strips were analysed for ten cytokines using Luminex Assay.</div></div><div><h3>Results</h3><div>56 % of participants fulfilled the TFOS DEWS II criteria for DED. 48 % had at least 25 % meibomian gland loss in either lid regardless of dry eye status, while over 90 % had at least one diagnostic sign. Progression was observed, characterised by significant increases (p < 0.05) in ocular redness, lid wiper epitheliopathy and blink rate. Albumin was upregulated (p = 0.003) in DED, while zinc-α2-glycoprotein, which showed significant correlations with several meibomian gland parameters, was downregulated. Upregulation of both pro- and anti-inflammatory cytokines was observed, with several significant clinical correlations, including IL-1β with meibomian gland parameters.</div></div><div><h3>Conclusions</h3><div>Evidence of inflammation and overlap of ocular signs in these young adults reinforces the need for early detection and differentiation of those likely to progress to DED. While upregulation of both pro- and anti-inflammatory cytokines has provided evidence of a mechanism to maintain homeostasis, the subtle progression of ocular surface disease observed suggests that counselling is required around the modifiable risk factors of DED identified, regardless of whether symptoms are present or not.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"37 ","pages":"Pages 57-67"},"PeriodicalIF":5.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of eyelid warming devices as first-step treatment in meibomian gland dysfunction: A systematic review with meta-analysis 眼睑温热装置作为睑板腺功能障碍第一步治疗的疗效：一项系统综述和meta分析。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-21 DOI: 10.1016/j.jtos.2025.02.008

Antonio Ballesteros-Sánchez , Carlos Rocha-de-Lossada , José-María Sánchez-González

Purpose

To investigate the efficacy of eyelid warming devices as first-step treatment in patients with meibomian gland dysfunction (MGD).

Methods

A systematic review with meta-analysis of RCTs, reporting the effects eyelid warming devices in 3 databases, PubMed, Scopus and Web of Science, was performed according to the PRISMA statement.

Results

Seven studies including 367 patients, and 440 eyes were analysed. The overall efficacy did not significantly favor either group when comparing eyelid warming devices to the control groups or warm towel compresses, nor between moisture chamber devices and warm compress devices. However, the change in OSDI questionnaire (SMD 0.91; 95 % CI: 0.44 to 1.39; P = 0.0002) and NIBUT (SMD 1.10; 95 % CI: 0.61 to 1.59; P < 0.0001) were significantly favorable for eyelid warming devices compared to the control groups. Similar results were obtained for tear film stability (SMD 0.97; 95 % CI: 0.32 to 1.61; P = 0.003) when comparing eyelid warming devices to warm towel compresses. Specifically, the sensitivity analysis of these groups revealed that changes in OSDI questionnaire (MD 9.41; 95 % CI: 1.70 to 17.13; P = 0.02; I² = 49 %) and NIBUT (MD 2.24; 95 % CI: 1.20 to 3.28; P < 0.0001; I² = 71 %) were significantly favorable for eyelid warming devices. When comparing moisture chamber devices and warm compress devices, only the change in TBUT (SMD 0.75; 95 % CI: 0.23 to 1.28; P = 0.005; I² = 30 %) were significantly favorable for moisture chamber goggles.

Conclusions

Despite their limited overall efficacy, eyelid warming devices significantly reduce OSDI questionnaire and improve NIBUT compared to controls or warm towel compress groups. Evidence remains insufficient to confirm whether moisture chamber devices provide better outcomes than warm compress devices. Further well-designed RCTs are needed to confirm these findings.

目的：探讨眼睑温热装置作为睑板腺功能障碍（MGD）患者第一步治疗的疗效。方法：根据PRISMA声明对PubMed、Scopus和Web of Science 3个数据库中报道眼睑升温装置效果的随机对照试验进行系统评价和meta分析。结果：分析了7项研究，367例患者，440只眼。当将眼睑加热装置与对照组或热毛巾进行比较时，或将湿室装置与热敷装置进行比较时，两组的总体效果都不明显。然而，OSDI问卷的变化(SMD 0.91；95% CI: 0.44 ~ 1.39；P = 0.0002)和NIBUT (SMD 1.10；95% CI: 0.61 ~ 1.59；P < 0.0001)明显优于对照组。泪膜稳定性(SMD 0.97；95% CI: 0.32 ~ 1.61；P = 0.003)。具体而言，这些组的敏感性分析显示，OSDI问卷的变化(MD 9.41；95% CI: 1.70 ~ 17.13；P = 0.02；I2 = 49%)和NIBUT (MD 2.24；95% CI: 1.20 ~ 3.28；P < 0.0001；I2 = 71%)明显支持眼睑温热装置。对比湿室装置和热敷装置，只有TBUT的变化(SMD 0.75；95% CI: 0.23 ~ 1.28；P = 0.005；I2 = 30%)显著有利于湿室护目镜。结论：尽管整体疗效有限，但与对照组或温巾敷组相比，眼睑温热装置可显著降低OSDI问卷，改善NIBUT。证据仍然不足以证实湿室装置是否比热敷装置提供更好的结果。需要进一步精心设计的随机对照试验来证实这些发现。

{"title":"Efficacy of eyelid warming devices as first-step treatment in meibomian gland dysfunction: A systematic review with meta-analysis","authors":"Antonio Ballesteros-Sánchez , Carlos Rocha-de-Lossada , José-María Sánchez-González","doi":"10.1016/j.jtos.2025.02.008","DOIUrl":"10.1016/j.jtos.2025.02.008","url":null,"abstract":"<div><h3>Purpose</h3><div>To investigate the efficacy of eyelid warming devices as first-step treatment in patients with meibomian gland dysfunction (MGD).</div></div><div><h3>Methods</h3><div>A systematic review with meta-analysis of RCTs, reporting the effects eyelid warming devices in 3 databases, PubMed, Scopus and Web of Science, was performed according to the PRISMA statement.</div></div><div><h3>Results</h3><div>Seven studies including 367 patients, and 440 eyes were analysed. The overall efficacy did not significantly favor either group when comparing eyelid warming devices to the control groups or warm towel compresses, nor between moisture chamber devices and warm compress devices. However, the change in OSDI questionnaire (SMD 0.91; 95 % CI: 0.44 to 1.39; P = 0.0002) and NIBUT (SMD 1.10; 95 % CI: 0.61 to 1.59; P < 0.0001) were significantly favorable for eyelid warming devices compared to the control groups. Similar results were obtained for tear film stability (SMD 0.97; 95 % CI: 0.32 to 1.61; P = 0.003) when comparing eyelid warming devices to warm towel compresses. Specifically, the sensitivity analysis of these groups revealed that changes in OSDI questionnaire (MD 9.41; 95 % CI: 1.70 to 17.13; <em>P</em> = 0.02; I<sup>2</sup> = 49 %) and NIBUT (MD 2.24; 95 % CI: 1.20 to 3.28; <em>P</em> < 0.0001; I<sup>2</sup> = 71 %) were significantly favorable for eyelid warming devices. When comparing moisture chamber devices and warm compress devices, only the change in TBUT (SMD 0.75; 95 % CI: 0.23 to 1.28; <em>P</em> = 0.005; I<sup>2</sup> = 30 %) were significantly favorable for moisture chamber goggles.</div></div><div><h3>Conclusions</h3><div>Despite their limited overall efficacy, eyelid warming devices significantly reduce OSDI questionnaire and improve NIBUT compared to controls or warm towel compress groups. Evidence remains insufficient to confirm whether moisture chamber devices provide better outcomes than warm compress devices. Further well-designed RCTs are needed to confirm these findings.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"37 ","pages":"Pages 33-46"},"PeriodicalIF":5.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of dry eye disease on patient-reported quality of life: A Save Sight Dry Eye Registry study 干眼病对患者报告的生活质量的影响：一项拯救视力干眼症登记研究。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-13 DOI: 10.1016/j.jtos.2025.02.005

Himal Kandel , Fiona Stapleton AO , Laura E. Downie , Ngozi C Chidi‐Egboka , David MIngo-Botin , Francisco Arnalich-Montiel , Saaeha Rauz , Alberto Recchioni , Sanjeeta Sitaula , Maria Markoulli , Vincent Daien , Fanny Babeau , Gerd Geerling , Jennifer P. Craig , Stephanie L. Watson OAM

Purpose

The aim of this real-world registry study was to evaluate the quality-of-life (QoL) impact of dry eye disease (DED). The specific objectives were to determine factors affecting QoL in DED, and to evaluate the psychometric properties of the Ocular Surface Disease Index (OSDI), Ocular Comfort Index (OCI), and Patient Health Questionnaire (PHQ) in a real-world DED population using modern psychometric methods.

Methods

This descriptive cross-sectional study included 368 DED patients (mean 54.7 ± 16.6 years; 80.2 % female) who completed one or more of the three questionnaires (OSDI, OCI and PHQ). Psychometric analysis of the QoL data was carried out with Andrich's Rating Scale Model of Rasch analysis.

Results

The original OSDI and OCI had disordered categories. The Rasch-optimised final QoL scales (OSDI – Overall, OSDI – Symptoms [SY], OSDI – Activity limitation [AL], OSDI – Environmental trigger [EN], OCI Overall, OCI - Frequency [FR], OCI - Intensity [IN], and PHQ) had satisfactory psychometric properties. Patients diagnosed with a mixed aqueous/evaporative DED subtype had worse mean OSDI-Overall and OSDI-AL scores than individuals with evaporative DED (p = 0.012 and 0.001 respectively). Patients with corneal neuropathic pain had worse QoL scores (OSDI-Overall, OSDI-AL, OSDI-SY, OSDI-EN, OCI-Overall, OCI-FR, OCI-IN, and PHQ; all p < 0.05) than those without. Patients who reported undergoing prior treatment or procedure for DED had worse QoL scores than those who did not (all p < 0.05 except for PHQ). The associations between DED signs (tear breakup time and staining) and symptoms were weak or not significant.

Conclusions

In this real-world setting, diagnoses of mixed DED, corneal neuropathic pain, and history of DED treatment/procedures were associated with worse dry eye symptoms, activity limitation, and/or QoL.

目的：本研究的目的是评估干眼病（DED）对生活质量（QoL）的影响。具体目的是确定影响DED患者生活质量的因素，并使用现代心理测量方法评估现实世界DED人群的眼表疾病指数（OSDI）、眼舒适度指数（OCI）和患者健康问卷（PHQ）的心理测量特性。方法：本描述性横断面研究纳入368例DED患者(平均54.7±16.6岁；80.2%女性)，完成三项问卷（OSDI、OCI和PHQ）中的一项或多项。生活质量数据采用Rasch分析的Andrich评定量表模型进行心理测量分析。结果：原始OSDI和OCI有紊乱分类。rasch优化的最终生活质量量表（OSDI - Overall, OSDI - symptom [SY], OSDI - Activity limitation [AL], OSDI - Environmental trigger [EN], OCI Overall, OCI- frequency [FR], OCI- intensity [IN] and PHQ）具有令人满意的心理测量特性。诊断为水/蒸发性DED混合亚型的患者的平均OSDI-Overall评分和OSDI-AL评分低于蒸发性DED个体（p分别= 0.012和0.001）。角膜神经性疼痛患者的生活质量评分较差(OSDI-Overall、OSDI-AL、OSDI-SY、OSDI-EN、OCI-Overall、OCI-FR、OCI-IN和PHQ；p < 0.05)。先前接受过DED治疗或手术的患者的生活质量评分比未接受治疗的患者差（除PHQ外，所有p < 0.05）。DED体征（泪液破裂时间和染色）与症状之间的相关性较弱或不显著。结论：在现实世界中，混合性DED的诊断、角膜神经性疼痛和DED治疗/手术史与更严重的干眼症状、活动限制和/或生活质量相关。

{"title":"The impact of dry eye disease on patient-reported quality of life: A Save Sight Dry Eye Registry study","authors":"Himal Kandel , Fiona Stapleton AO , Laura E. Downie , Ngozi C Chidi‐Egboka , David MIngo-Botin , Francisco Arnalich-Montiel , Saaeha Rauz , Alberto Recchioni , Sanjeeta Sitaula , Maria Markoulli , Vincent Daien , Fanny Babeau , Gerd Geerling , Jennifer P. Craig , Stephanie L. Watson OAM","doi":"10.1016/j.jtos.2025.02.005","DOIUrl":"10.1016/j.jtos.2025.02.005","url":null,"abstract":"<div><h3>Purpose</h3><div>The aim of this real-world registry study was to evaluate the quality-of-life (QoL) impact of dry eye disease (DED). The specific objectives were to determine factors affecting QoL in DED, and to evaluate the psychometric properties of the Ocular Surface Disease Index (OSDI), Ocular Comfort Index (OCI), and Patient Health Questionnaire (PHQ) in a real-world DED population using modern psychometric methods.</div></div><div><h3>Methods</h3><div>This descriptive cross-sectional study included 368 DED patients (mean 54.7 ± 16.6 years; 80.2 % female) who completed one or more of the three questionnaires (OSDI, OCI and PHQ). Psychometric analysis of the QoL data was carried out with Andrich's Rating Scale Model of Rasch analysis.</div></div><div><h3>Results</h3><div>The original OSDI and OCI had disordered categories. The Rasch-optimised final QoL scales (OSDI – Overall, OSDI – Symptoms [SY], OSDI – Activity limitation [AL], OSDI – Environmental trigger [EN], OCI Overall, OCI - Frequency [FR], OCI - Intensity [IN], and PHQ) had satisfactory psychometric properties. Patients diagnosed with a mixed aqueous/evaporative DED subtype had worse mean OSDI-Overall and OSDI-AL scores than individuals with evaporative DED (p = 0.012 and 0.001 respectively). Patients with corneal neuropathic pain had worse QoL scores (OSDI-Overall, OSDI-AL, OSDI-SY, OSDI-EN, OCI-Overall, OCI-FR, OCI-IN, and PHQ; all p < 0.05) than those without. Patients who reported undergoing prior treatment or procedure for DED had worse QoL scores than those who did not (all p < 0.05 except for PHQ). The associations between DED signs (tear breakup time and staining) and symptoms were weak or not significant.</div></div><div><h3>Conclusions</h3><div>In this real-world setting, diagnoses of mixed DED, corneal neuropathic pain, and history of DED treatment/procedures were associated with worse dry eye symptoms, activity limitation, and/or QoL.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"37 ","pages":"Pages 11-23"},"PeriodicalIF":5.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of botulinum toxin type A on ocular pain with neuropathic features A型肉毒毒素对具有神经性特征的眼痛的影响。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-12 DOI: 10.1016/j.jtos.2025.02.007

Elyana V.T. Locatelli , Jaxon J. Huang , Jason D. Betz , Jordan J. Huang , Nicole B. Kantor , Nicholas Reyes , Elizabeth R. Felix , Wendy W. Lee , Anat Galor

Purpose

To investigate the impact of botulinum toxin type A (BoNT-A) on neuropathic/nociplastic ocular pain (NOP) and identify predictors of patient outcomes.

Methods

A retrospective study of individuals with NOP symptoms (light sensitivity, wind/air sensitivity, persistent pain despite dry eye treatment) who received ≥1 BoNT-A injection. Primary outcome measures included if (responders vs. non-responders) and to what degree (none vs. mild vs. moderate vs. marked) individuals experienced pain improvement 4–6 weeks post-injection. Demographics and clinical exam information was compared between the groups.

Results

27 individuals received BoNT-A for NOP symptoms. 74 % (n = 20) reported an improvement in pain and were classified as responders. Among responders, the degree of benefit varied, with 25 % reporting mild, 45 % moderate, and 30 % marked pain improvement. Improvements in light sensitivity (37 %), wind/air sensitivity (33 %), and quality of life (QoL) (59 %) were reported by fewer individuals. 80 % of responders and 0 % of non-responders reported QoL improvements afer BoNT-A. In a multivariable model that examined predictors of response (none to marked, 0–3), the presence of fibromyalgia (FM) (β = 0.50; p = 0.004) portended a better response, while shooting pain (β = −0.47; p = 0.007) portended a worse response to BoNT-A, (full model r² = 0.53; p < 0.001). Degree of pain improvement significantly correlated with improvements in light sensitivity, wind/air sensitivity, and QoL (ρ range: 0.42–0.63; p < 0.05).

Conclusion

After BoNT-A, most individuals reported improved ocular pain and QoL, while fewer noted improved light and wind/air sensitivity. Some systemic and ocular factors predicted treatment response and may thus guide treatment.

目的：探讨A型肉毒毒素（BoNT-A）对神经性/伤害性眼痛（NOP）的影响，并确定患者预后的预测因素。方法：回顾性研究接受≥1次BoNT-A注射的有NOP症状（光敏感、风/空气敏感、干眼治疗后持续疼痛）的个体。主要结果测量包括注射后4-6周个体是否（有反应者vs无反应者）以及疼痛改善程度（无反应者vs轻度反应者vs中度反应者vs明显反应者）。比较两组间的人口统计学和临床检查信息。结果：27例患者接受BoNT-A治疗NOP症状。74% （n=20）的患者报告疼痛有所改善，并被归类为应答者。在应答者中，获益程度各不相同，25%报告轻度，45%报告中度，30%报告显着疼痛改善。光敏感性（37%）、风/空气敏感性（33%）和生活质量（QoL）（59%）的改善报告个体较少。80%的应答者和0%的无应答者报告BoNT-A后生活质量有所改善。在检验反应预测因子的多变量模型中（无标记，0-3），纤维肌痛（FM）的存在(β=0.50；P =0.004)预示着更好的反应，而射击痛(β=-0.47；p=0.007)预示着BoNT-A的反应更差，(全模型r2=0.53；结论：在BoNT-A后，大多数人报告眼痛和生活质量得到改善，而较少的人报告光和风/空气敏感性得到改善。一些系统和眼部因素可以预测治疗反应，从而指导治疗。

{"title":"Impact of botulinum toxin type A on ocular pain with neuropathic features","authors":"Elyana V.T. Locatelli , Jaxon J. Huang , Jason D. Betz , Jordan J. Huang , Nicole B. Kantor , Nicholas Reyes , Elizabeth R. Felix , Wendy W. Lee , Anat Galor","doi":"10.1016/j.jtos.2025.02.007","DOIUrl":"10.1016/j.jtos.2025.02.007","url":null,"abstract":"<div><h3>Purpose</h3><div>To investigate the impact of botulinum toxin type A (BoNT-A) on neuropathic/nociplastic ocular pain (NOP) and identify predictors of patient outcomes.</div></div><div><h3>Methods</h3><div>A retrospective study of individuals with NOP symptoms (light sensitivity, wind/air sensitivity, persistent pain despite dry eye treatment) who received ≥1 BoNT-A injection. Primary outcome measures included if (responders vs. non-responders) and to what degree (none vs. mild vs. moderate vs. marked) individuals experienced pain improvement 4–6 weeks post-injection. Demographics and clinical exam information was compared between the groups.</div></div><div><h3>Results</h3><div>27 individuals received BoNT-A for NOP symptoms. 74 % (n = 20) reported an improvement in pain and were classified as responders. Among responders, the degree of benefit varied, with 25 % reporting mild, 45 % moderate, and 30 % marked pain improvement. Improvements in light sensitivity (37 %), wind/air sensitivity (33 %), and quality of life (QoL) (59 %) were reported by fewer individuals. 80 % of responders and 0 % of non-responders reported QoL improvements afer BoNT-A. In a multivariable model that examined predictors of response (none to marked, 0–3), the presence of fibromyalgia (FM) (β = 0.50; p = 0.004) portended a better response, while shooting pain (β = −0.47; p = 0.007) portended a worse response to BoNT-A, (full model r<sup>2</sup> = 0.53; p < 0.001). Degree of pain improvement significantly correlated with improvements in light sensitivity, wind/air sensitivity, and QoL (ρ range: 0.42–0.63; p < 0.05).</div></div><div><h3>Conclusion</h3><div>After BoNT-A, most individuals reported improved ocular pain and QoL, while fewer noted improved light and wind/air sensitivity. Some systemic and ocular factors predicted treatment response and may thus guide treatment.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"37 ","pages":"Pages 24-32"},"PeriodicalIF":5.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the clonal growth potential of meibomian gland stem/progenitor cells via clonal analysis 通过克隆分析评估睑板腺干/祖细胞的克隆生长潜力。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-12 DOI: 10.1016/j.jtos.2025.02.006

Yuli Guo , Rongrong Zhang , Minjie Zhang , Sai Luo , Wansui Li , Le Sun , Meiqin Zhong , Zuguo Liu , Yang Wu , Wei Li , Jinghua Bu

Purpose

Clonal analysis is a feasible method to evaluate the status of stem/progenitor cells in epidermal or limbus investigations. This study aimed to evaluate the clonal growth potential of meibomian gland (MG) epithelial cells using clonal analysis.

Methods

Mouse and human MG tissues were isolated and cocultured with 3T3 feeder cells. Immunofluorescent staining of K14, K6a, and PPARγ on MG clones was applied. Holoclones, meroclones and paraclones were categorized based on clonal area. Triple staining and tile scans provided a comprehensive view of MG clone formation. MG ductal and acinar clones were cultured separately to compare stem/progenitor cell characteristics. We further evaluated an age-related MGD (ARMGD) mouse model along with two human MG samples of different ages using clonal analysis. Crystal violet staining was employed to assess clone formation efficiency (CFE).

Results

Both mouse and human MG epithelial cells formed clones on the feeder layers, which enlarged over time. The expression of K14, K6a, and PPARγ was decreased in differentiated clones during development. The CFE of holoclones and meroclones was approximately 1 ‰ in mouse MG clones and approximately 2.5 ‰ in holoclones and 5.6 ‰ in meroclones in human MG clones. The CFE of holoclones generated by ductal epithelial cells was significantly higher than did acinar clones. In the ARMGD mouse model and human samples, smaller clones, reduced CFE, and decreased K14+, K6a+, and PPARγ+ cells in MG clones were identified.

Conclusions

Clonal analysis effectively evaluates stem and progenitor cells in MGs, revealing deterioration in these cells under MGD conditions.

目的：克隆分析是评价干细胞/祖细胞在表皮或角膜缘研究中的状态的一种可行方法。本研究旨在通过克隆分析评价睑板腺（MG）上皮细胞的克隆生长潜力。方法：分离小鼠和人MG组织，与3T3饲养细胞共培养。对MG克隆进行K14、K6a和PPARγ免疫荧光染色。根据克隆面积对全息克隆、分裂克隆和仿克隆进行分类。三重染色和瓦片扫描提供了MG克隆形成的全面视图。MG导管克隆和腺泡克隆分别培养，比较干细胞/祖细胞特性。我们使用克隆分析进一步评估了年龄相关性MGD （ARMGD）小鼠模型以及两个不同年龄的人MG样本。结晶紫染色法评价克隆形成效率（CFE）。结果：小鼠和人MG上皮细胞均在饲养层上形成克隆，且随时间增大。分化无性系中K14、K6a和PPARγ的表达在发育过程中降低。小鼠MG克隆中全息克隆和mero克隆的CFE约为1‰，人MG克隆中全息克隆和mero克隆的CFE约为2.5‰和5.6‰。由导管上皮细胞生成的全息克隆的CFE显著高于腺泡克隆。在ARMGD小鼠模型和人类样本中，MG克隆的克隆更小，CFE减少，K14+、K6a+和PPARγ+细胞减少。结论：克隆分析可以有效地评估MGD中干细胞和祖细胞，揭示这些细胞在MGD条件下的恶化。

{"title":"Assessment of the clonal growth potential of meibomian gland stem/progenitor cells via clonal analysis","authors":"Yuli Guo , Rongrong Zhang , Minjie Zhang , Sai Luo , Wansui Li , Le Sun , Meiqin Zhong , Zuguo Liu , Yang Wu , Wei Li , Jinghua Bu","doi":"10.1016/j.jtos.2025.02.006","DOIUrl":"10.1016/j.jtos.2025.02.006","url":null,"abstract":"<div><h3>Purpose</h3><div>Clonal analysis is a feasible method to evaluate the status of stem/progenitor cells in epidermal or limbus investigations. This study aimed to evaluate the clonal growth potential of meibomian gland (MG) epithelial cells using clonal analysis.</div></div><div><h3>Methods</h3><div>Mouse and human MG tissues were isolated and cocultured with 3T3 feeder cells. Immunofluorescent staining of K14, K6a, and PPARγ on MG clones was applied. Holoclones, meroclones and paraclones were categorized based on clonal area. Triple staining and tile scans provided a comprehensive view of MG clone formation. MG ductal and acinar clones were cultured separately to compare stem/progenitor cell characteristics. We further evaluated an age-related MGD (ARMGD) mouse model along with two human MG samples of different ages using clonal analysis. Crystal violet staining was employed to assess clone formation efficiency (CFE).</div></div><div><h3>Results</h3><div>Both mouse and human MG epithelial cells formed clones on the feeder layers, which enlarged over time. The expression of K14, K6a, and PPARγ was decreased in differentiated clones during development. The CFE of holoclones and meroclones was approximately 1 ‰ in mouse MG clones and approximately 2.5 ‰ in holoclones and 5.6 ‰ in meroclones in human MG clones. The CFE of holoclones generated by ductal epithelial cells was significantly higher than did acinar clones. In the ARMGD mouse model and human samples, smaller clones, reduced CFE, and decreased K14+, K6a+, and PPARγ+ cells in MG clones were identified.</div></div><div><h3>Conclusions</h3><div>Clonal analysis effectively evaluates stem and progenitor cells in MGs, revealing deterioration in these cells under MGD conditions.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"37 ","pages":"Pages 1-10"},"PeriodicalIF":5.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alleviative effect of probiotics and prebiotics on dry eye in type 2 diabetic mice through the gut-eye axis 益生菌和益生元通过肠眼轴对2型糖尿病小鼠干眼的缓解作用。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-06 DOI: 10.1016/j.jtos.2025.02.004

Shirui Dai , Jianfeng Long , Wentao Han , Liwei Zhang , Baihua Chen

Diabetes Mellitus (DM) is a metabolic disease that manifests as a state of “chronic low-grade inflammation”. Patients with DM have a disorder of intestinal flora. There is a discernible correlation between this disorder of intestinal flora and the onset and progression of eye diseases, which offers novel insights into treating eye diseases through the modulation of intestinal flora. Here, we demonstrated that a high-fat diet and streptozotocin injection-induced intestinal microbiota dysbiosis can lead to dry eye-like manifestations in T2DM mice. Probiotic and prebiotic treatments not only alleviated intestinal inflammation and barrier disruption, but also mitigated damage to the lacrimal barrier and suppressed immune cell infiltration and inflammatory responses. Additional mechanism investigation found that probiotics and prebiotics inhibited the TLR4/NF-κB signaling pathway and its downstream pro-inflammatory products both in the lacrimal gland and colon. 16S RNA sequencing identified a reduction in the bacterial genera Akkermansia and Lactobacillus in the fecal samples of DM mice. By contrast, treatment with probiotics and prebiotics led to a reshaping of the intestinal microbial community and a reduction in bile acid metabolites, such as taurocholic acid and deoxycholic acid. Our current study demonstrates that probiotic and prebiotic treatments can ameliorate dry eye-like symptoms and associated pathological changes in T2DM mice. Moreover, we proved that a high-fat diet and STZ-induced microbiota dysbiosis were involved in diabetic dry eye through the gut-eye axis.

糖尿病是一种代谢性疾病，表现为“慢性低度炎症”状态。糖尿病患者有肠道菌群紊乱。这种肠道菌群紊乱与眼部疾病的发生和发展之间存在明显的相关性，这为通过调节肠道菌群治疗眼部疾病提供了新的见解。在这里，我们证明了高脂肪饮食和注射链脲佐菌素诱导的肠道微生物群失调可导致T2DM小鼠的干眼样表现。益生菌和益生元治疗不仅可以减轻肠道炎症和屏障破坏，还可以减轻泪道屏障的损伤，抑制免疫细胞浸润和炎症反应。进一步的机制研究发现，益生菌和益生元可抑制泪腺和结肠中TLR4/NF-κB信号通路及其下游促炎产物。16S RNA测序鉴定出DM小鼠粪便样本中Akkermansia和乳杆菌属细菌的减少。相比之下，使用益生菌和益生元治疗导致肠道微生物群落的重塑和胆汁酸代谢物的减少，如牛磺胆酸和脱氧胆酸。我们目前的研究表明，益生菌和益生元治疗可以改善2型糖尿病小鼠的干眼样症状和相关病理改变。此外，我们证明了高脂肪饮食和stz诱导的微生物群失调通过肠眼轴参与糖尿病干眼症。

{"title":"Alleviative effect of probiotics and prebiotics on dry eye in type 2 diabetic mice through the gut-eye axis","authors":"Shirui Dai , Jianfeng Long , Wentao Han , Liwei Zhang , Baihua Chen","doi":"10.1016/j.jtos.2025.02.004","DOIUrl":"10.1016/j.jtos.2025.02.004","url":null,"abstract":"<div><div>Diabetes Mellitus (DM) is a metabolic disease that manifests as a state of “chronic low-grade inflammation”. Patients with DM have a disorder of intestinal flora. There is a discernible correlation between this disorder of intestinal flora and the onset and progression of eye diseases, which offers novel insights into treating eye diseases through the modulation of intestinal flora. Here, we demonstrated that a high-fat diet and streptozotocin injection-induced intestinal microbiota dysbiosis can lead to dry eye-like manifestations in T2DM mice. Probiotic and prebiotic treatments not only alleviated intestinal inflammation and barrier disruption, but also mitigated damage to the lacrimal barrier and suppressed immune cell infiltration and inflammatory responses. Additional mechanism investigation found that probiotics and prebiotics inhibited the TLR4/NF-κB signaling pathway and its downstream pro-inflammatory products both in the lacrimal gland and colon. 16S RNA sequencing identified a reduction in the bacterial genera <em>Akkermansia</em> and <em>Lactobacillus</em> in the fecal samples of DM mice. By contrast, treatment with probiotics and prebiotics led to a reshaping of the intestinal microbial community and a reduction in bile acid metabolites, such as taurocholic acid and deoxycholic acid. Our current study demonstrates that probiotic and prebiotic treatments can ameliorate dry eye-like symptoms and associated pathological changes in T2DM mice. Moreover, we proved that a high-fat diet and STZ-induced microbiota dysbiosis were involved in diabetic dry eye through the gut-eye axis.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"36 ","pages":"Pages 244-260"},"PeriodicalIF":5.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of blinking exercises on palpebral fissure height and tear film parameters 眨眼运动对睑裂高度和泪膜参数的影响。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-05 DOI: 10.1016/j.jtos.2025.02.003

Reiko Arita , Shima Fukuoka , Ray Matsumoto , Minako Kaido

Purpose

Blinking is an involuntary movement essential for ocular surface health and visual comfort. While blinking exercises in patients with dry eye have been shown to improve symptoms, increase non-invasive tear film breakup time (NIBUT), and decrease incomplete blink rate (IBR), no studies have quantified improvements in eyelid opening. This study evaluated the effects of blinking exercises on palpebral fissure height (PFH), subjective symptoms, and tear film-related parameters.

Methods

Participants were randomly assigned to a “blinking exercise group” that performed blinking exercises after instilling artificial tear drops five times daily for three days or control group that only used artificial tear drops. Standard Patient Evaluation of Eye Dryness (SPEED) and Visual Analog Scale (VAS) scores were recorded for dryness, eye strain, ocular discomfort, blurred vision, foreign body sensation, dullness, and difficulty in opening the eyelids. The pre- and post-study measurements included lipid layer thickness, PFH, blink interval, IBR, tear meniscus height, NIBUT, fluorescein staining, and fluorescein breakup time (FBUT).

Results

Among 100 participants (28 males, 72 females, mean age 38.4 ± 7.4 years), 52 were in the blinking exercise group and 48 were in the control group. The blinking exercise group showed significant improvements in SPEED (P < 0.001), VAS scores for eye strain and discomfort (P = 0.003, 0.007), enlarged PFH (P < 0.001), prolonged NIBUT and FBUT (P < 0.001), and reduced IBR (P < 0.001) compared to the controls.

Conclusions

Blinking exercises improved PFH, incomplete blinking, tear film stability, and subjective symptoms in patients with dry eye.

目的：眨眼是一种无意识的运动，对眼表健康和视觉舒适至关重要。虽然干眼症患者的眨眼运动已被证明可以改善症状，增加非侵入性泪膜破裂时间（NIBUT），并降低不完全眨眼率（IBR），但没有研究量化眼皮打开的改善。本研究评估眨眼运动对睑裂高度（PFH）、主观症状和泪膜相关参数的影响。方法：参与者被随机分为“眨眼运动组”，每天5次滴入人工泪液后进行眨眼运动，持续3天；对照组只使用人工泪液。记录干燥、眼疲劳、眼不适、视力模糊、异物感、暗沉、睁眼困难的标准患者眼干涩评价（SPEED）和视觉模拟量表（VAS）评分。研究前后的测量包括脂质层厚度、PFH、眨眼间隔、IBR、撕裂半月板高度、NIBUT、荧光素染色和荧光素分解时间（FBUT）。结果：100例受试者中，男性28例，女性72例，平均年龄38.4±7.4岁，眨眼运动组52例，对照组48例。与对照组相比，眨眼运动组在SPEED （P < 0.001）、眼疲劳和不适VAS评分（P = 0.003、0.007）、PFH增大（P < 0.001）、NIBUT和FBUT延长（P < 0.001）和IBR降低（P < 0.001）方面均有显著改善。结论：眨眼运动可改善干眼症患者的PFH、不完全眨眼、泪膜稳定性和主观症状。

{"title":"Effects of blinking exercises on palpebral fissure height and tear film parameters","authors":"Reiko Arita , Shima Fukuoka , Ray Matsumoto , Minako Kaido","doi":"10.1016/j.jtos.2025.02.003","DOIUrl":"10.1016/j.jtos.2025.02.003","url":null,"abstract":"<div><h3>Purpose</h3><div>Blinking is an involuntary movement essential for ocular surface health and visual comfort. While blinking exercises in patients with dry eye have been shown to improve symptoms, increase non-invasive tear film breakup time (NIBUT), and decrease incomplete blink rate (IBR), no studies have quantified improvements in eyelid opening. This study evaluated the effects of blinking exercises on palpebral fissure height (PFH), subjective symptoms, and tear film-related parameters.</div></div><div><h3>Methods</h3><div>Participants were randomly assigned to a “blinking exercise group” that performed blinking exercises after instilling artificial tear drops five times daily for three days or control group that only used artificial tear drops. Standard Patient Evaluation of Eye Dryness (SPEED) and Visual Analog Scale (VAS) scores were recorded for dryness, eye strain, ocular discomfort, blurred vision, foreign body sensation, dullness, and difficulty in opening the eyelids. The pre- and post-study measurements included lipid layer thickness, PFH, blink interval, IBR, tear meniscus height, NIBUT, fluorescein staining, and fluorescein breakup time (FBUT).</div></div><div><h3>Results</h3><div>Among 100 participants (28 males, 72 females, mean age 38.4 ± 7.4 years), 52 were in the blinking exercise group and 48 were in the control group. The blinking exercise group showed significant improvements in SPEED (<em>P</em> < 0.001), VAS scores for eye strain and discomfort (<em>P</em> = 0.003, 0.007), enlarged PFH (<em>P</em> < 0.001), prolonged NIBUT and FBUT (<em>P</em> < 0.001), and reduced IBR (<em>P</em> < 0.001) compared to the controls.</div></div><div><h3>Conclusions</h3><div>Blinking exercises improved PFH, incomplete blinking, tear film stability, and subjective symptoms in patients with dry eye.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"36 ","pages":"Pages 237-243"},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and optimization of an ex vivo model of corneal epithelium damage with 1-heptanol: Investigating the influence of donor clinical parameters and MSC-sEV treatment on healing capacity 1-庚醇角膜上皮损伤离体模型的建立与优化：研究供体临床参数和MSC-sEV治疗对愈合能力的影响

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-02-04 DOI: 10.1016/j.jtos.2025.02.002

Filippo Bonelli , Seyedmohammad Moosavizadeh , Elisa Fasolo , Alessia Di Nella , Vanessa Barbaro , Ilaria Zorzi , Mauro Krampera , Jana D'Amato Tóthová , Diego Ponzin , Thomas Ritter , Stefano Ferrari , Umberto Rodella

Purpose

To develop and characterize a reproducible human corneal epithelial wound-healing model using 1-heptanol, and to investigate the healing potential of Bone Marrow-derived Mesenchymal Stromal Cell small Extracellular Vesicles (MSC-sEV) and the influence of donor characteristics on epithelial healing.

Methods

Eighty-eight (n = 88) human corneoscleral tissues unsuitable for transplantation were employed. Corneal epithelial damage was induced with 1-heptanol and monitored every 24 h up to 96 h using fluorescein and trypan blue staining. Histological assessment was performed on untreated and damaged tissues. Damaged areas were measured with FIJI software, and healing rates were calculated. MSC-sEV were isolated with size exclusion chromatography and characterized for their size, morphology and biomarkers. Their impact on healing was assessed in both in vitro scratch assays on cultured human corneal epithelial cells and on ex vivo 1-heptanol-damaged corneas.

Results

Histological analysis revealed detached corneal epithelium in the central area, while other layers remained unaffected. Healing rate peaked at 48 h post-damage. Trypan blue and Fluorescein staining correlated and the former highlighted a higher initial healing rate than the latter. Diabetic and heart-beating brain-deceased donors showed impaired healing rates. MSC-sEV (79.8 nm, spherical bilayer, positive for TSG101, CD9, CD63, and CD81) significantly improved epithelial wound healing in both in vitro and ex vivo models.

Conclusion

1-heptanol effectively induces reproducible corneal epithelial damage, and the ex vivo organ-cultured human cornea heals the epithelium within 96 h. Diabetes and donation from heart-beating brain-deceased donors reduce healing capacity. MSC-sEV boost epithelial repair in damaged corneas.

目的：利用1-庚醇建立可复制的人角膜上皮细胞创面愈合模型并对其进行表征，探讨骨髓间充质基质细胞小胞外囊泡（MSC-sEV）的愈合潜力以及供体特征对上皮愈合的影响。方法：采用88例不适合移植的人角膜巩膜组织。用1-庚醇诱导角膜上皮损伤，每24小时监测一次，直至96小时用荧光素和台盼蓝染色。对未处理和受损组织进行组织学评估。用斐济软件测量损伤面积，计算愈合率。采用大小排斥色谱法分离MSC-sEV，并对其大小、形态和生物标志物进行了表征。在培养的人角膜上皮细胞和离体1-庚醇损伤的角膜的体外划伤实验中，评估了它们对愈合的影响。结果：组织学分析显示角膜中央区角膜上皮脱落，而其他层未受影响。愈合率在伤害后48小时达到峰值。台盼蓝染色与荧光素染色相关，前者的初始愈合率高于后者。糖尿病患者和心脏跳动的脑死亡捐赠者的治愈率受损。mscs - sev （79.8 nm，球形双层，TSG101, CD9， CD63和CD81阳性）在体外和离体模型中均显著改善上皮伤口愈合。结论：1-庚醇能有效诱导可复制性角膜上皮损伤，体外器官培养的人角膜可在96小时内愈合。糖尿病和心脏跳动的脑死亡捐赠者的捐赠会降低治疗能力。MSC-sEV促进受损角膜上皮修复。

{"title":"Development and optimization of an ex vivo model of corneal epithelium damage with 1-heptanol: Investigating the influence of donor clinical parameters and MSC-sEV treatment on healing capacity","authors":"Filippo Bonelli , Seyedmohammad Moosavizadeh , Elisa Fasolo , Alessia Di Nella , Vanessa Barbaro , Ilaria Zorzi , Mauro Krampera , Jana D'Amato Tóthová , Diego Ponzin , Thomas Ritter , Stefano Ferrari , Umberto Rodella","doi":"10.1016/j.jtos.2025.02.002","DOIUrl":"10.1016/j.jtos.2025.02.002","url":null,"abstract":"<div><h3>Purpose</h3><div>To develop and characterize a reproducible human corneal epithelial wound-healing model using 1-heptanol, and to investigate the healing potential of Bone Marrow-derived Mesenchymal Stromal Cell small Extracellular Vesicles (MSC-sEV) and the influence of donor characteristics on epithelial healing.</div></div><div><h3>Methods</h3><div>Eighty-eight (n = 88) human corneoscleral tissues unsuitable for transplantation were employed. Corneal epithelial damage was induced with 1-heptanol and monitored every 24 h up to 96 h using fluorescein and trypan blue staining. Histological assessment was performed on untreated and damaged tissues. Damaged areas were measured with FIJI software, and healing rates were calculated. MSC-sEV were isolated with size exclusion chromatography and characterized for their size, morphology and biomarkers. Their impact on healing was assessed in both <em>in vitro</em> scratch assays on cultured human corneal epithelial cells and on <em>ex vivo</em> 1-heptanol-damaged corneas.</div></div><div><h3>Results</h3><div>Histological analysis revealed detached corneal epithelium in the central area, while other layers remained unaffected. Healing rate peaked at 48 h post-damage. Trypan blue and Fluorescein staining correlated and the former highlighted a higher initial healing rate than the latter. Diabetic and heart-beating brain-deceased donors showed impaired healing rates. MSC-sEV (79.8 nm, spherical bilayer, positive for TSG101, CD9, CD63, and CD81) significantly improved epithelial wound healing in both <em>in vitro</em> and <em>ex vivo</em> models.</div></div><div><h3>Conclusion</h3><div>1-heptanol effectively induces reproducible corneal epithelial damage, and the <em>ex vivo</em> organ-cultured human cornea heals the epithelium within 96 h. Diabetes and donation from heart-beating brain-deceased donors reduce healing capacity. MSC-sEV boost epithelial repair in damaged corneas.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"36 ","pages":"Pages 224-236"},"PeriodicalIF":5.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0