Ocular Surface最新文献_第10页

Antibiotic resistance of Pseudomonas aeruginosa from ocular samples in Canada: a 25-year analysis 加拿大眼部样本中铜绿假单胞菌的抗生素耐药性：25年分析。

IF 5.6 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-30 DOI: 10.1016/j.jtos.2025.07.011

Alfonso Iovieno , Barbara Burgos-Blasco , Derek Chan , Sean Ling , Simon Holland , Sonia N. Yeung

Objective

To identify trends in antibiotic resistance patterns of Pseudomonas aeruginosa (PA) over a multi-decade period and analyse clinical outcomes.

Methods

PA isolates from ocular samples were collected over a 25-year time period in British Columbia, Canada. Source and antibiotic resistance were recorded. Demographic data, previous ocular history and clinical outcomes of patients with PA keratitis were analysed and compared in moxifloxacin-resistant versus moxifloxacin-sensitive PA keratitis cases.

Results

321 ocular isolates of Pseudomonas were identified, 297 (92.5 %) of which were PA (101, 34 % cornea samples). Resistance to chloramphenicol was 88.7 %, 23.7 % to moxifloxacin and 10.1 % to meropenem. An increase in moxifloxacin resistance and non-susceptibility was noted, with a decrease in tobramycin resistance. The same trend was not observed for other fluroquinolone antibiotics. 14 (4.7 %) PA isolates were multi drug-resistant. 53 PA corneal isolates were included in the clinical analysis: 21 (40 %) sensitive, 17 (32 %) intermediate and 15 (28 %) resistant to moxifloxacin. Non-susceptible PA patients were older, more frequently non-contact lens wearers and a had a higher prevalence of previous ocular surgeries and topical treatment. A lower clinical response to initial treatment was observed in resistant cases. Need for surgery and complications were higher among moxifloxacin-resistant cases.

Conclusions

This study reports for the first time in North America a progressive and significant increase in moxifloxacin resistance among PA isolates. Overuse of topical moxifloxacin may be underlying this finding. Given the worse clinical outcomes in resistant cases, we would caution against the use of moxifloxacin alone as empirical treatment of infectious keratitis.

目的：确定铜绿假单胞菌（PA）耐药性模式的趋势，并分析其临床结果。方法：从加拿大不列颠哥伦比亚省的眼部样本中收集了25年的PA分离物。记录来源及抗生素耐药性。分析并比较莫西沙星耐药和莫西沙星敏感的PA角膜炎患者的人口统计学资料、既往眼部病史和临床结果。结果：共检出321株假单胞菌，其中PA 297株（92.5%），占角膜标本的101.34%。氯霉素耐药率为88.7%，莫西沙星耐药率为23.7%，美罗培南耐药率为10.1%。莫西沙星耐药和非敏感性增加，妥布霉素耐药减少。其他氟喹诺酮类抗生素没有观察到同样的趋势。14株（4.7%）PA分离株多重耐药。临床分析53株PA角膜分离株：对莫西沙星敏感21株（40%），中间17株（32%），耐药15株（28%）。非易感PA患者年龄较大，经常佩戴非隐形眼镜，既往眼部手术和局部治疗的患病率较高。耐药病例对初始治疗的临床反应较低。莫西沙星耐药病例的手术需求和并发症较高。结论：本研究首次在北美报道了PA分离株中莫西沙星耐药性的进展性和显著性增加。过量使用局部莫西沙星可能是这一发现的基础。鉴于耐药病例的临床结果较差，我们建议不要单独使用莫西沙星作为感染性角膜炎的经验性治疗。

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引用次数: 0

Topical pharmacologic treatments for dry eye disease: A systematic review 干眼病的局部药物治疗：系统综述。

IF 5.6 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-30 DOI: 10.1016/j.jtos.2025.07.010

Louis Tong , Zuguo Liu , Afsun Şahin , Koray Gümüş , Elisabeth M. Messmer , José M. Benítez-del-Castillo , Marc Labetoulle , Clara C. Chan , Laura M. Periman

Background

Topical pharmacologic treatments for dry eye disease (DED) address different aspects of tear film deficiency by decreasing ocular surface inflammation, stimulating mucin secretion, increasing tear production, or reducing excessive evaporation. This systematic review evaluated randomized controlled trials (RCTs) and prospective observational studies of topical ophthalmic medications for DED.

Methods

PubMed and Embase were searched from 1980 to February 2024. For studies meeting inclusion criteria, efficacy outcomes (signs and symptoms of DED) and adverse event data were extracted.

Results

A total of 107 publications covering topical prescription medications (anti-inflammatory agents cyclosporine and lifitegrast; mucin secretagogues diquafosol and rebamipide; tear evaporation inhibitor perfluorohexyloctane; tear production stimulator nasal spray varenicline), other commercially available products, and novel agents in development were identified. In RCTs, significant improvements relative to a control group were demonstrated more often for sign endpoints (e.g., corneal staining, Schirmer score) than for symptom endpoints (e.g., eye dryness, ocular discomfort). The evaluated treatments were well tolerated; instillation site reactions were the most commonly reported adverse events. Year-long safety extension studies demonstrated maintenance of efficacy, with no new safety signals identified. Studies differed in design, methodology, control group, and outcomes assessment, making it difficult to compare across products, and head-to-head studies were rare. Several new products are in late-stage development, which will likely lead to additional treatment options.

Conclusions

Current topical pharmacologic eye products improved signs, and sometimes symptoms, of DED and were well tolerated. Treatment selection should use a shared decision-making approach that takes DED etiology and patient preferences into account.

背景：干眼病（DED）的局部药物治疗通过减少眼表炎症、刺激黏液分泌、增加泪液产生或减少过度蒸发来解决泪膜缺乏的不同方面。本系统综述评价了局部眼科药物治疗DED的随机对照试验（rct）和前瞻性观察性研究。方法：检索1980 ~ 2024年2月PubMed和Embase数据库。对于符合纳入标准的研究，提取疗效结局（DED的体征和症状）和不良事件数据。结果：共有107篇出版物涵盖了外用处方药(抗炎药环孢素和利替格司；粘蛋白分泌剂：双喹福醇和利巴米胺；撕裂蒸发抑制剂全氟己辛烷；确定了泪液产生刺激剂（varenicline鼻喷雾剂），其他市售产品和正在开发的新型药物。在随机对照试验中，与对照组相比，体征终点（如角膜染色、Schirmer评分）比症状终点（如眼睛干燥、眼部不适）更常表现出显著的改善。经评估的治疗方法耐受性良好；注射部位反应是最常见的不良事件。为期一年的安全性扩展研究表明，该药物维持了疗效，未发现新的安全信号。研究在设计、方法、对照组和结果评估方面存在差异，这使得很难对不同产品进行比较，而且针锋相对的研究很少。一些新产品正处于后期开发阶段，这可能会带来更多的治疗选择。结论：目前的局部药物眼科产品改善了DED的体征，有时是症状，并且耐受性良好。治疗选择应采用共同决策的方法，将DED病因和患者偏好考虑在内。

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引用次数: 0

New insight into the neuroimmune interplay in Pseudomonas aeruginosa keratitis 铜绿假单胞菌性角膜炎的神经免疫相互作用新认识

IF 5.6 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-24 DOI: 10.1016/j.jtos.2025.07.008

Naman Gupta, Giovanni LoGrasso, Linda D. Hazlett, Shunbin Xu

Purpose

This research aims to reveal the roles of the miR-183/96/182 cluster (miR-183C) in sensory neurons (SN) in the interplay of corneal sensory nerves (CSN) and myeloid cells (MC) during Pseudomonas aeruginosa (PA) keratitis.

Methods

The left corneas of SN-specific (SNS) conditional knockout (CKO) and their wild type (WT) littermates were infected with PA. CSN of these mice express RFP; MC EGFP. Confocal microscopy of corneal flatmount, myeloperoxidase (MPO) assay and plate count were performed.

Results

In WT mice, CSN began to degenerate at 3 h-post-infection (hpi), starting from epithelial/subepithelial layers in the central region. By 1 day-post-infection (dpi), epithelium/subepithelial CSN were nearly completely destroyed, while stromal nerves persisted. From 3 dpi, CSN were obliterated in both layers. In CKO vs WT mice, CSN density was decreased at 3 and 6 hpi; however, CNS degeneration followed a slower pace. At 3 dpi, residual large-diameter stromal CSN were better preserved.

MC were decreased in the central cornea at 3 and 6 hpi, but increased in the periphery, more prominent in CKO mice. At 12 hpi, densely packed MC formed a ring-shaped band circling a “dark” zone nearly devoid of MC, colocalizing with CSN most degenerated central area. At 1 dpi, the cornea was filled with MC; MC density was lower in CKO. CKO mice had decreased neutrophils at 1 dpi and reduced disease severity at 3 dpi.

Conclusions

Our results provide new insight into the neuroimmune interplay during PA keratitis. miR-183C in CSN modulates PA keratitis through regulation of neuroimmune interaction.

目的：本研究旨在揭示在铜绿假单胞菌（PA）性角膜炎中，感觉神经元中miR-183/96/182簇（miR-183C）在角膜感觉神经（CSN）和髓样细胞（MC）相互作用中的作用。方法：对sn特异性（SNS）条件敲除（CKO）的左眼角膜及其野生型（WT）幼崽进行PA感染。这些小鼠的CSN表达RFP；MC EGFP。角膜平片共聚焦显微镜，髓过氧化物酶（MPO）测定和平板计数。结果：在WT小鼠中，CSN在感染后3小时（hpi）开始变性，从中央区域的上皮/上皮下层开始。感染后1天（dpi），上皮/上皮下CSN几乎完全破坏，而间质神经持续存在。从3 dpi开始，两层的CSN均被抹去。在CKO和WT小鼠中，CSN密度在3和6 hpi时降低；然而，中枢神经系统退化的速度较慢。3 dpi时，残余大直径间质CSN保存较好。在3和6 hpi时，中央角膜MC减少，而周围角膜MC增加，在CKO小鼠中更为明显。在12 hpi时，密集的MC形成一个环状带，环绕着一个几乎没有MC的“黑暗”区，与CSN最退化的中心区共域。在1 dpi时，角膜充满MC；CKO组MC密度较低。CKO小鼠在1 dpi时中性粒细胞减少，在3 dpi时疾病严重程度降低。结论：我们的研究结果为PA角膜炎的神经免疫相互作用提供了新的见解。CSN中的miR-183C通过调节神经免疫相互作用调节PA角膜炎。

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引用次数: 0

Systemic immune checkpoint inhibitors: Successful treatment of conjunctival atypical melanocytic proliferation documented by anterior segment optical coherence tomography 系统性免疫检查点抑制剂：成功治疗结膜非典型黑素细胞增生的前段光学相干断层扫描记录

IF 5.6 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-19 DOI: 10.1016/j.jtos.2025.07.005

Gelila B. Yohannes , Nathan L. Scott , Wendy J. Li , Carol L. Karp

引用次数: 0

Corneal sensory nerve regulation of tear production through stimulation of transient receptor potential melastatin 8 (TRPM8) channel: A potential new approach for treating dry eye disease 角膜感觉神经通过刺激瞬时受体电位美拉他汀8 （TRPM8）通道调节泪液产生：一种治疗干眼病的潜在新方法

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-18 DOI: 10.1016/j.jtos.2025.07.003

Juana Gallar , Stephen Pflugfelder , Anat Galor , Preeya K. Gupta , Pedram Hamrah

The lacrimal functional unit (LFU) tightly controls the secretion of all tear components, thus playing a critical role in maintaining ocular surface homeostasis. Forming an exquisitely sensitive neural network across the ocular surface, corneal sensory nerves detect environmental stimuli (e.g., temperature, chemicals, and mechanical pressure) through transient receptor potential (TRP) ion channels. Among these, TRP melastatin 8 (TRPM8) is a key regulator of basal tear production. Stimulated by the small temperature reductions and tear film osmolarity increases that arise due to evaporative cooling, TRPM8 activates the LFU, leading to increased basal tear production.

Here, we focus on reviewing the topical ocular pathways within the LFU that regulate tear production. We describe the neural signaling underlying this regulation, with a focus on TRP channels and the central role of TRPM8 in basal tear production as elucidated through preclinical as well as limited clinical evidence. Lastly, we explore how augmenting the fundamental action of TRPM8 signaling through agonist stimulation may serve as a valuable new treatment option for dry eye disease.

泪功能单位（LFU）严格控制所有泪液成分的分泌，因此在维持眼表稳态中起关键作用。角膜感觉神经通过瞬时受体电位（TRP）离子通道，在眼表面形成一个非常敏感的神经网络，检测环境刺激（如温度、化学物质和机械压力）。其中，TRP美拉抑素8 （TRPM8）是基础泪液产生的关键调节因子。在蒸发冷却引起的温度降低和泪膜渗透压增加的刺激下，TRPM8激活LFU，导致基础泪液产量增加。

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引用次数: 0

Prostaglandin F2α exacerbated dry eye by promoting lacrimal gland fibrosis progression through the activation of the RhoA/ROCKs signaling pathway 前列腺素F2α通过激活RhoA/ROCKs信号通路，促进泪腺纤维化进展，从而加重干眼症

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-18 DOI: 10.1016/j.jtos.2025.07.004

Shujia Guo , Jiayu Kang , Ke Yan , Jiani Li , Ruochen Wang , Danyi Qin , Yuqian Wang , Yuwen Liu , Wenying Guan , Han Wu , Jiaoyue Hu , Wei Li , Yongxiong Chen , Caihong Huang , Zuguo Liu

Purpose

To investigate Prostaglandin F2α (PGF2α) expression in dry eye (DE) patients and its correlation with clinical manifestations, exploring potential mechanisms in DE.

Methods

Cross-sectional case-control study including 21 DE patients and 16 controls. PGF2α levels were detected by ELISA (Enzyme-linked immunosorbent assay). Correlation analyses were conducted between PGF2α in human tears and DE symptoms (The Ocular Surface Disease Index, OSDI) or signs including tear film breakup time using fluorescein sodium strips (FBUT), Schirmer Ⅰ test (ST) and corneal fluorescein staining (CFS). Dry eye models were induced using scopolamine and desiccating stress, with transcriptomic sequencing to analyze differential gene expression. DE mice were treated with the PGF2α receptor inhibitor AL8810. Various assays (Oregon green dextran staining, phenol red thread test, PCR, immunofluorescence, MASSON staining, ELISA, Western Blot) evaluated DE phenotypes, lacrimal gland inflammation, and fibrosis.

Results

DE patients had significantly elevated PGF2α levels, negatively correlating with ST and positively with CFS. DE mice showed increased PGF2α and FP receptor expression in lacrimal glands, decreased tear production, worsening ocular surface damage, and elevated inflammation and fibrosis. The TGFβ1/Smads and RhoA/ROCKs pathways were activated, with changes becoming more pronounced with extended molding time. AL8810 reduced fibrosis, partially restored tear secretion, and alleviated corneal damage while inhibiting RhoA/ROCKs pathway activation without affecting TGFβ1 expression.

Conclusions

PGF2α exacerbated DE by promoting lacrimal gland fibrosis progression via the RhoA/ROCKs signaling pathway. Inhibiting PGF2α receptors effectively suppressed the progression of dry eye and lacrimal gland fibrosis, which may offer a promising therapeutic strategy for DE, particularly in refractory cases associated with lacrimal gland fibrosis.

探讨干眼症（DE）患者前列腺素F2α (PGF2α)的表达及其与临床表现的相关性，探讨干眼症的发病机制。

{"title":"Prostaglandin F2α exacerbated dry eye by promoting lacrimal gland fibrosis progression through the activation of the RhoA/ROCKs signaling pathway","authors":"Shujia Guo , Jiayu Kang , Ke Yan , Jiani Li , Ruochen Wang , Danyi Qin , Yuqian Wang , Yuwen Liu , Wenying Guan , Han Wu , Jiaoyue Hu , Wei Li , Yongxiong Chen , Caihong Huang , Zuguo Liu","doi":"10.1016/j.jtos.2025.07.004","DOIUrl":"10.1016/j.jtos.2025.07.004","url":null,"abstract":"<div><h3>Purpose</h3><div>To investigate Prostaglandin F2α (PGF2α) expression in dry eye (DE) patients and its correlation with clinical manifestations, exploring potential mechanisms in DE.</div></div><div><h3>Methods</h3><div>Cross-sectional case-control study including 21 DE patients and 16 controls. PGF2α levels were detected by ELISA (Enzyme-linked immunosorbent assay). Correlation analyses were conducted between PGF2α in human tears and DE symptoms (The Ocular Surface Disease Index, OSDI) or signs including tear film breakup time using fluorescein sodium strips (FBUT), Schirmer Ⅰ test (ST) and corneal fluorescein staining (CFS). Dry eye models were induced using scopolamine and desiccating stress, with transcriptomic sequencing to analyze differential gene expression. DE mice were treated with the PGF2α receptor inhibitor AL8810. Various assays (Oregon green dextran staining, phenol red thread test, PCR, immunofluorescence, MASSON staining, ELISA, Western Blot) evaluated DE phenotypes, lacrimal gland inflammation, and fibrosis.</div></div><div><h3>Results</h3><div>DE patients had significantly elevated PGF2α levels, negatively correlating with ST and positively with CFS. DE mice showed increased PGF2α and FP receptor expression in lacrimal glands, decreased tear production, worsening ocular surface damage, and elevated inflammation and fibrosis. The TGFβ1/Smads and RhoA/ROCKs pathways were activated, with changes becoming more pronounced with extended molding time. AL8810 reduced fibrosis, partially restored tear secretion, and alleviated corneal damage while inhibiting RhoA/ROCKs pathway activation without affecting TGFβ1 expression.</div></div><div><h3>Conclusions</h3><div>PGF2α exacerbated DE by promoting lacrimal gland fibrosis progression via the RhoA/ROCKs signaling pathway. Inhibiting PGF2α receptors effectively suppressed the progression of dry eye and lacrimal gland fibrosis, which may offer a promising therapeutic strategy for DE, particularly in refractory cases associated with lacrimal gland fibrosis.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"38 ","pages":"Pages 155-169"},"PeriodicalIF":5.9,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144664961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early myeloid cell infiltration and subset-specific macrophage responses in murine corneal nerve injury 小鼠角膜神经损伤的早期髓样细胞浸润和亚群特异性巨噬细胞反应

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-10 DOI: 10.1016/j.jtos.2025.07.001

Seung Hyeun Lee , Soo Jin Lee , Ahra Koh , Yunjin Lee , Seonghan Kim , Suil Jeon , Noseong Park , Chang Ho Yoon , Ki Hean Kim , Kyoung Woo Kim

Purpose

Corneal nerve fibers and resident macrophages form a specialized microenvironment essential for tissue integrity and recovery after injury. This study aims to elucidate the early immune dynamics following corneal nerve injury, focusing on myeloid cell infiltration and resident macrophage subset shifts.

Methods

Using a murine circular nerve-cut model, we tracked immune responses for 21 days, with a focus on the first 12 h post-injury. Confocal imaging was used to assess corneal nerve density, while flow cytometry quantified infiltrating and resident immune cell populations. Transcriptomic profiling was performed at 3 and 6 h post-injury to analyze inflammatory gene expression, and in vitro experiments examined the effects of short-term nerve growth factor (NGF) exposure on macrophage polarization.

Results

Confocal imaging showed a rapid decrease in corneal nerve density, followed by progressive regeneration. Flow cytometry revealed a surge in Ly6C⁺ myeloid cells at 3–6 h post-injury, predominantly in the central cornea, with an early tendency toward M2-like polarization. Resident macrophages exhibited distinct responses: M2-like and undifferentiated subsets declined, while M1-like cells were proportionally maintained, indicating divergent but complementary roles during the initial inflammatory phase. Transcriptomic profiling showed significant upregulation of inflammatory genes along with a transient increase in Ngf and compensatory anti-inflammatory signaling. In vitro, short-term NGF exposure enhanced both M1-and M2-like polarization, mirroring in vivo activation patterns.

Conclusion

Early myeloid cell infiltration and macrophage subset dynamics contribute to the initial neuroinflammatory response and may influence subsequent repair processes, highlighting the potential for immune modulation in corneal nerve regeneration.

角膜神经纤维和常驻巨噬细胞形成了一个特殊的微环境，对损伤后组织的完整性和恢复至关重要。本研究旨在阐明角膜神经损伤后的早期免疫动力学，重点关注骨髓细胞浸润和常驻巨噬细胞亚群转移。

{"title":"Early myeloid cell infiltration and subset-specific macrophage responses in murine corneal nerve injury","authors":"Seung Hyeun Lee , Soo Jin Lee , Ahra Koh , Yunjin Lee , Seonghan Kim , Suil Jeon , Noseong Park , Chang Ho Yoon , Ki Hean Kim , Kyoung Woo Kim","doi":"10.1016/j.jtos.2025.07.001","DOIUrl":"10.1016/j.jtos.2025.07.001","url":null,"abstract":"<div><h3>Purpose</h3><div>Corneal nerve fibers and resident macrophages form a specialized microenvironment essential for tissue integrity and recovery after injury. This study aims to elucidate the early immune dynamics following corneal nerve injury, focusing on myeloid cell infiltration and resident macrophage subset shifts.</div></div><div><h3>Methods</h3><div>Using a murine circular nerve-cut model, we tracked immune responses for 21 days, with a focus on the first 12 h post-injury. Confocal imaging was used to assess corneal nerve density, while flow cytometry quantified infiltrating and resident immune cell populations. Transcriptomic profiling was performed at 3 and 6 h post-injury to analyze inflammatory gene expression, and <em>in vitro</em> experiments examined the effects of short-term nerve growth factor (NGF) exposure on macrophage polarization.</div></div><div><h3>Results</h3><div>Confocal imaging showed a rapid decrease in corneal nerve density, followed by progressive regeneration. Flow cytometry revealed a surge in Ly6C<sup>+</sup> myeloid cells at 3–6 h post-injury, predominantly in the central cornea, with an early tendency toward M2-like polarization. Resident macrophages exhibited distinct responses: M2-like and undifferentiated subsets declined, while M1-like cells were proportionally maintained, indicating divergent but complementary roles during the initial inflammatory phase. Transcriptomic profiling showed significant upregulation of inflammatory genes along with a transient increase in <em>Ngf</em> and compensatory anti-inflammatory signaling. <em>In vitro</em>, short-term NGF exposure enhanced both M1-and M2-like polarization, mirroring <em>in vivo</em> activation patterns.</div></div><div><h3>Conclusion</h3><div>Early myeloid cell infiltration and macrophage subset dynamics contribute to the initial neuroinflammatory response and may influence subsequent repair processes, highlighting the potential for immune modulation in corneal nerve regeneration.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"38 ","pages":"Pages 117-131"},"PeriodicalIF":5.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specificity of direct immunofluorescence in healthy conjunctival specimens 直接免疫荧光在健康结膜标本中的特异性。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-06-25 DOI: 10.1016/j.jtos.2025.06.003

Anahita Kate , Poornima Golthi , Swapna S. Shanbhag , Megana Peddi , Simmy Chaudhary , Saumya Jakati , Sayan Basu

引用次数: 0

Moxifloxacin-resistant and moxifloxacin-susceptible Staphylococcus aureus Keratitis: Outcomes from a 10-year retrospective study 莫西沙星耐药和莫西沙星敏感的金黄色葡萄球菌角膜炎：10年回顾性研究的结果

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-06-23 DOI: 10.1016/j.jtos.2025.06.008

Raven Diacou , Rohan Bir Singh , Eric G. Romanowski , Jonathan B. Mandell , Alex Mammen , Robert M.Q. Shanks , Vishal Jhanji

Purpose

Fluoroquinolones, specifically moxifloxacin are commonly used to treat bacterial keratitis. However, recent clinical evidence assessing the clinical outcomes in bacterial keratitis cases resistant to moxifloxacin remains sparse. This study evaluates the clinical characteristics and outcomes in cases of Staphylococcus aureus keratitis with in vitro resistance to moxifloxacin.

Design

Retrospective clinical cohort study.

Methods

Keratitis patients with cultures positive for Staphylococcus aureus at the University of Pittsburgh Medical Center, were identified between July 2012 and June 2022.

Results

A total of 104 patients with culture-confirmed Staphylococcus aureus keratitis were included in the study. Patients infected by moxifloxacin-resistant bacteria (n = 32) were significantly older (74.32 ± 17.41 years) than moxifloxacin-susceptible infections (55.56 ± 20.86 years, p < 0.0001). Moxifloxacin resistance was identified in 29.8 % of cases, including methicillin-susceptible (4.8 %) and methicillin-resistant Staphylococcus aureus (25.96 %) isolates. The most common risk factors for moxifloxacin resistance were ocular surface disease (35.5 %) and history of prior infection (32.2 %). Moxifloxacin-resistant Staphylococcus aureus was associated with larger epithelial defects (18.18 ± 4.93 mm² vs. 5.10 ± 0.76 mm², p < 0.0001), longer healing times (42.42 ± 6.75 days vs. 32.2 ± 3.56 days, p < 0.0001), and higher rates of corneal perforation (19.3 % vs. 4.2 %; p = 0.0317). Visual acuity outcomes were significantly worse in the resistant group, with minimal improvement from baseline (1.87 ± 0.14 LogMAR) to final follow-up (1.8 ± 0.18 LogMAR), compared to the moxifloxacin-susceptible group (from 1.46 ± 0.11 LogMAR to 1.15 ± 0.11 LogMAR, p < 0.0001). Enucleation was required in 9.6 % of resistant cases.

Conclusions

The current study determined significant differences in clinical characteristics and outcomes among corneal ulcers caused by moxifloxacin-resistant Staphylococcus aureus.

目的氟喹诺酮类药物，特别是莫西沙星常用于治疗细菌性角膜炎。然而，最近评估细菌性角膜炎对莫西沙星耐药的临床结果的临床证据仍然很少。本研究对莫西沙星体外耐药金黄色葡萄球菌角膜炎的临床特点和预后进行了评价。设计回顾性临床队列研究。方法选取2012年7月至2022年6月在匹兹堡大学医学中心发现的金黄色葡萄球菌培养阳性的角膜炎患者。结果共纳入104例经培养证实的金黄色葡萄球菌角膜炎患者。莫西沙星耐药菌感染患者（n = 32）年龄（74.32±17.41岁）明显大于莫西沙星敏感菌感染患者(55.56±20.86岁，p <；0.0001)。29.8%的病例耐莫西沙星，其中甲氧西林敏感（4.8%）和耐甲氧西林金黄色葡萄球菌（25.96%）。莫西沙星耐药最常见的危险因素是眼表疾病（35.5%）和既往感染史（32.2%）。耐莫西沙星金黄色葡萄球菌与较大的上皮缺损相关(18.18±4.93 mm2 vs. 5.10±0.76 mm2, p <；0.0001)，较长的愈合时间(42.42±6.75天vs. 32.2±3.56天，p <；0.0001)，角膜穿孔率较高(19.3% vs. 4.2%；p = 0.0317)。与莫西沙星敏感组（从1.46±0.11 LogMAR到1.15±0.11 LogMAR）相比，耐药组的视力结果明显更差，从基线（1.87±0.14 LogMAR）到最终随访（1.8±0.18 LogMAR）的改善极小(p <；0.0001)。9.6%的耐药病例需要去核。结论本研究确定了耐莫西沙星金黄色葡萄球菌引起的角膜溃疡的临床特征和结局存在显著差异。

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引用次数: 0

Corrigendum to “Models for Meibomian gland dysfunction: In vivo and in vitro”, Review article [Ocul. Surf. 32 (2024) 154–165] “睑板腺功能障碍模型：体内和体外”的勘误表。综述文章，眼表32(2024)154-165。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-06-23 DOI: 10.1016/j.jtos.2025.06.009

Jinghua Bu , Yuli Guo , Yang Wu , Rongrong Zhang , Jingbin Zhuang , Jiankai Zhao , Le Sun , Andrew J. Quantock , Zuguo Liu , Wei Li

引用次数: 0