Ocular Surface最新文献

英文中文

New insight into the neuroimmune interplay in Pseudomonas aeruginosa keratitis 铜绿假单胞菌性角膜炎的神经免疫相互作用新认识

IF 5.6 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-24 DOI: 10.1016/j.jtos.2025.07.008

Naman Gupta, Giovanni LoGrasso, Linda D. Hazlett, Shunbin Xu

Purpose

This research aims to reveal the roles of the miR-183/96/182 cluster (miR-183C) in sensory neurons (SN) in the interplay of corneal sensory nerves (CSN) and myeloid cells (MC) during Pseudomonas aeruginosa (PA) keratitis.

Methods

The left corneas of SN-specific (SNS) conditional knockout (CKO) and their wild type (WT) littermates were infected with PA. CSN of these mice express RFP; MC EGFP. Confocal microscopy of corneal flatmount, myeloperoxidase (MPO) assay and plate count were performed.

Results

In WT mice, CSN began to degenerate at 3 h-post-infection (hpi), starting from epithelial/subepithelial layers in the central region. By 1 day-post-infection (dpi), epithelium/subepithelial CSN were nearly completely destroyed, while stromal nerves persisted. From 3 dpi, CSN were obliterated in both layers. In CKO vs WT mice, CSN density was decreased at 3 and 6 hpi; however, CNS degeneration followed a slower pace. At 3 dpi, residual large-diameter stromal CSN were better preserved.

MC were decreased in the central cornea at 3 and 6 hpi, but increased in the periphery, more prominent in CKO mice. At 12 hpi, densely packed MC formed a ring-shaped band circling a “dark” zone nearly devoid of MC, colocalizing with CSN most degenerated central area. At 1 dpi, the cornea was filled with MC; MC density was lower in CKO. CKO mice had decreased neutrophils at 1 dpi and reduced disease severity at 3 dpi.

Conclusions

Our results provide new insight into the neuroimmune interplay during PA keratitis. miR-183C in CSN modulates PA keratitis through regulation of neuroimmune interaction.

目的：本研究旨在揭示在铜绿假单胞菌（PA）性角膜炎中，感觉神经元中miR-183/96/182簇（miR-183C）在角膜感觉神经（CSN）和髓样细胞（MC）相互作用中的作用。方法：对sn特异性（SNS）条件敲除（CKO）的左眼角膜及其野生型（WT）幼崽进行PA感染。这些小鼠的CSN表达RFP；MC EGFP。角膜平片共聚焦显微镜，髓过氧化物酶（MPO）测定和平板计数。结果：在WT小鼠中，CSN在感染后3小时（hpi）开始变性，从中央区域的上皮/上皮下层开始。感染后1天（dpi），上皮/上皮下CSN几乎完全破坏，而间质神经持续存在。从3 dpi开始，两层的CSN均被抹去。在CKO和WT小鼠中，CSN密度在3和6 hpi时降低；然而，中枢神经系统退化的速度较慢。3 dpi时，残余大直径间质CSN保存较好。在3和6 hpi时，中央角膜MC减少，而周围角膜MC增加，在CKO小鼠中更为明显。在12 hpi时，密集的MC形成一个环状带，环绕着一个几乎没有MC的“黑暗”区，与CSN最退化的中心区共域。在1 dpi时，角膜充满MC；CKO组MC密度较低。CKO小鼠在1 dpi时中性粒细胞减少，在3 dpi时疾病严重程度降低。结论：我们的研究结果为PA角膜炎的神经免疫相互作用提供了新的见解。CSN中的miR-183C通过调节神经免疫相互作用调节PA角膜炎。

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引用次数: 0

Systemic immune checkpoint inhibitors: Successful treatment of conjunctival atypical melanocytic proliferation documented by anterior segment optical coherence tomography 系统性免疫检查点抑制剂：成功治疗结膜非典型黑素细胞增生的前段光学相干断层扫描记录

IF 5.6 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-19 DOI: 10.1016/j.jtos.2025.07.005

Gelila B. Yohannes , Nathan L. Scott , Wendy J. Li , Carol L. Karp

引用次数: 0

Corneal sensory nerve regulation of tear production through stimulation of transient receptor potential melastatin 8 (TRPM8) channel: A potential new approach for treating dry eye disease 角膜感觉神经通过刺激瞬时受体电位美拉他汀8 （TRPM8）通道调节泪液产生：一种治疗干眼病的潜在新方法

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-18 DOI: 10.1016/j.jtos.2025.07.003

Juana Gallar , Stephen Pflugfelder , Anat Galor , Preeya K. Gupta , Pedram Hamrah

The lacrimal functional unit (LFU) tightly controls the secretion of all tear components, thus playing a critical role in maintaining ocular surface homeostasis. Forming an exquisitely sensitive neural network across the ocular surface, corneal sensory nerves detect environmental stimuli (e.g., temperature, chemicals, and mechanical pressure) through transient receptor potential (TRP) ion channels. Among these, TRP melastatin 8 (TRPM8) is a key regulator of basal tear production. Stimulated by the small temperature reductions and tear film osmolarity increases that arise due to evaporative cooling, TRPM8 activates the LFU, leading to increased basal tear production.

Here, we focus on reviewing the topical ocular pathways within the LFU that regulate tear production. We describe the neural signaling underlying this regulation, with a focus on TRP channels and the central role of TRPM8 in basal tear production as elucidated through preclinical as well as limited clinical evidence. Lastly, we explore how augmenting the fundamental action of TRPM8 signaling through agonist stimulation may serve as a valuable new treatment option for dry eye disease.

泪功能单位（LFU）严格控制所有泪液成分的分泌，因此在维持眼表稳态中起关键作用。角膜感觉神经通过瞬时受体电位（TRP）离子通道，在眼表面形成一个非常敏感的神经网络，检测环境刺激（如温度、化学物质和机械压力）。其中，TRP美拉抑素8 （TRPM8）是基础泪液产生的关键调节因子。在蒸发冷却引起的温度降低和泪膜渗透压增加的刺激下，TRPM8激活LFU，导致基础泪液产量增加。

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引用次数: 0

Prostaglandin F2α exacerbated dry eye by promoting lacrimal gland fibrosis progression through the activation of the RhoA/ROCKs signaling pathway 前列腺素F2α通过激活RhoA/ROCKs信号通路，促进泪腺纤维化进展，从而加重干眼症

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-18 DOI: 10.1016/j.jtos.2025.07.004

Shujia Guo , Jiayu Kang , Ke Yan , Jiani Li , Ruochen Wang , Danyi Qin , Yuqian Wang , Yuwen Liu , Wenying Guan , Han Wu , Jiaoyue Hu , Wei Li , Yongxiong Chen , Caihong Huang , Zuguo Liu

Purpose

To investigate Prostaglandin F2α (PGF2α) expression in dry eye (DE) patients and its correlation with clinical manifestations, exploring potential mechanisms in DE.

Methods

Cross-sectional case-control study including 21 DE patients and 16 controls. PGF2α levels were detected by ELISA (Enzyme-linked immunosorbent assay). Correlation analyses were conducted between PGF2α in human tears and DE symptoms (The Ocular Surface Disease Index, OSDI) or signs including tear film breakup time using fluorescein sodium strips (FBUT), Schirmer Ⅰ test (ST) and corneal fluorescein staining (CFS). Dry eye models were induced using scopolamine and desiccating stress, with transcriptomic sequencing to analyze differential gene expression. DE mice were treated with the PGF2α receptor inhibitor AL8810. Various assays (Oregon green dextran staining, phenol red thread test, PCR, immunofluorescence, MASSON staining, ELISA, Western Blot) evaluated DE phenotypes, lacrimal gland inflammation, and fibrosis.

Results

DE patients had significantly elevated PGF2α levels, negatively correlating with ST and positively with CFS. DE mice showed increased PGF2α and FP receptor expression in lacrimal glands, decreased tear production, worsening ocular surface damage, and elevated inflammation and fibrosis. The TGFβ1/Smads and RhoA/ROCKs pathways were activated, with changes becoming more pronounced with extended molding time. AL8810 reduced fibrosis, partially restored tear secretion, and alleviated corneal damage while inhibiting RhoA/ROCKs pathway activation without affecting TGFβ1 expression.

Conclusions

PGF2α exacerbated DE by promoting lacrimal gland fibrosis progression via the RhoA/ROCKs signaling pathway. Inhibiting PGF2α receptors effectively suppressed the progression of dry eye and lacrimal gland fibrosis, which may offer a promising therapeutic strategy for DE, particularly in refractory cases associated with lacrimal gland fibrosis.

探讨干眼症（DE）患者前列腺素F2α (PGF2α)的表达及其与临床表现的相关性，探讨干眼症的发病机制。

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引用次数: 0

Early myeloid cell infiltration and subset-specific macrophage responses in murine corneal nerve injury 小鼠角膜神经损伤的早期髓样细胞浸润和亚群特异性巨噬细胞反应

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-07-10 DOI: 10.1016/j.jtos.2025.07.001

Seung Hyeun Lee , Soo Jin Lee , Ahra Koh , Yunjin Lee , Seonghan Kim , Suil Jeon , Noseong Park , Chang Ho Yoon , Ki Hean Kim , Kyoung Woo Kim

Purpose

Corneal nerve fibers and resident macrophages form a specialized microenvironment essential for tissue integrity and recovery after injury. This study aims to elucidate the early immune dynamics following corneal nerve injury, focusing on myeloid cell infiltration and resident macrophage subset shifts.

Methods

Using a murine circular nerve-cut model, we tracked immune responses for 21 days, with a focus on the first 12 h post-injury. Confocal imaging was used to assess corneal nerve density, while flow cytometry quantified infiltrating and resident immune cell populations. Transcriptomic profiling was performed at 3 and 6 h post-injury to analyze inflammatory gene expression, and in vitro experiments examined the effects of short-term nerve growth factor (NGF) exposure on macrophage polarization.

Results

Confocal imaging showed a rapid decrease in corneal nerve density, followed by progressive regeneration. Flow cytometry revealed a surge in Ly6C⁺ myeloid cells at 3–6 h post-injury, predominantly in the central cornea, with an early tendency toward M2-like polarization. Resident macrophages exhibited distinct responses: M2-like and undifferentiated subsets declined, while M1-like cells were proportionally maintained, indicating divergent but complementary roles during the initial inflammatory phase. Transcriptomic profiling showed significant upregulation of inflammatory genes along with a transient increase in Ngf and compensatory anti-inflammatory signaling. In vitro, short-term NGF exposure enhanced both M1-and M2-like polarization, mirroring in vivo activation patterns.

Conclusion

Early myeloid cell infiltration and macrophage subset dynamics contribute to the initial neuroinflammatory response and may influence subsequent repair processes, highlighting the potential for immune modulation in corneal nerve regeneration.

角膜神经纤维和常驻巨噬细胞形成了一个特殊的微环境，对损伤后组织的完整性和恢复至关重要。本研究旨在阐明角膜神经损伤后的早期免疫动力学，重点关注骨髓细胞浸润和常驻巨噬细胞亚群转移。

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引用次数: 0

Specificity of direct immunofluorescence in healthy conjunctival specimens 直接免疫荧光在健康结膜标本中的特异性。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-06-25 DOI: 10.1016/j.jtos.2025.06.003

Anahita Kate , Poornima Golthi , Swapna S. Shanbhag , Megana Peddi , Simmy Chaudhary , Saumya Jakati , Sayan Basu

引用次数: 0

Moxifloxacin-resistant and moxifloxacin-susceptible Staphylococcus aureus Keratitis: Outcomes from a 10-year retrospective study 莫西沙星耐药和莫西沙星敏感的金黄色葡萄球菌角膜炎：10年回顾性研究的结果

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-06-23 DOI: 10.1016/j.jtos.2025.06.008

Raven Diacou , Rohan Bir Singh , Eric G. Romanowski , Jonathan B. Mandell , Alex Mammen , Robert M.Q. Shanks , Vishal Jhanji

Purpose

Fluoroquinolones, specifically moxifloxacin are commonly used to treat bacterial keratitis. However, recent clinical evidence assessing the clinical outcomes in bacterial keratitis cases resistant to moxifloxacin remains sparse. This study evaluates the clinical characteristics and outcomes in cases of Staphylococcus aureus keratitis with in vitro resistance to moxifloxacin.

Design

Retrospective clinical cohort study.

Methods

Keratitis patients with cultures positive for Staphylococcus aureus at the University of Pittsburgh Medical Center, were identified between July 2012 and June 2022.

Results

A total of 104 patients with culture-confirmed Staphylococcus aureus keratitis were included in the study. Patients infected by moxifloxacin-resistant bacteria (n = 32) were significantly older (74.32 ± 17.41 years) than moxifloxacin-susceptible infections (55.56 ± 20.86 years, p < 0.0001). Moxifloxacin resistance was identified in 29.8 % of cases, including methicillin-susceptible (4.8 %) and methicillin-resistant Staphylococcus aureus (25.96 %) isolates. The most common risk factors for moxifloxacin resistance were ocular surface disease (35.5 %) and history of prior infection (32.2 %). Moxifloxacin-resistant Staphylococcus aureus was associated with larger epithelial defects (18.18 ± 4.93 mm² vs. 5.10 ± 0.76 mm², p < 0.0001), longer healing times (42.42 ± 6.75 days vs. 32.2 ± 3.56 days, p < 0.0001), and higher rates of corneal perforation (19.3 % vs. 4.2 %; p = 0.0317). Visual acuity outcomes were significantly worse in the resistant group, with minimal improvement from baseline (1.87 ± 0.14 LogMAR) to final follow-up (1.8 ± 0.18 LogMAR), compared to the moxifloxacin-susceptible group (from 1.46 ± 0.11 LogMAR to 1.15 ± 0.11 LogMAR, p < 0.0001). Enucleation was required in 9.6 % of resistant cases.

Conclusions

The current study determined significant differences in clinical characteristics and outcomes among corneal ulcers caused by moxifloxacin-resistant Staphylococcus aureus.

目的氟喹诺酮类药物，特别是莫西沙星常用于治疗细菌性角膜炎。然而，最近评估细菌性角膜炎对莫西沙星耐药的临床结果的临床证据仍然很少。本研究对莫西沙星体外耐药金黄色葡萄球菌角膜炎的临床特点和预后进行了评价。设计回顾性临床队列研究。方法选取2012年7月至2022年6月在匹兹堡大学医学中心发现的金黄色葡萄球菌培养阳性的角膜炎患者。结果共纳入104例经培养证实的金黄色葡萄球菌角膜炎患者。莫西沙星耐药菌感染患者（n = 32）年龄（74.32±17.41岁）明显大于莫西沙星敏感菌感染患者(55.56±20.86岁，p <；0.0001)。29.8%的病例耐莫西沙星，其中甲氧西林敏感（4.8%）和耐甲氧西林金黄色葡萄球菌（25.96%）。莫西沙星耐药最常见的危险因素是眼表疾病（35.5%）和既往感染史（32.2%）。耐莫西沙星金黄色葡萄球菌与较大的上皮缺损相关(18.18±4.93 mm2 vs. 5.10±0.76 mm2, p <；0.0001)，较长的愈合时间(42.42±6.75天vs. 32.2±3.56天，p <；0.0001)，角膜穿孔率较高(19.3% vs. 4.2%；p = 0.0317)。与莫西沙星敏感组（从1.46±0.11 LogMAR到1.15±0.11 LogMAR）相比，耐药组的视力结果明显更差，从基线（1.87±0.14 LogMAR）到最终随访（1.8±0.18 LogMAR）的改善极小(p <；0.0001)。9.6%的耐药病例需要去核。结论本研究确定了耐莫西沙星金黄色葡萄球菌引起的角膜溃疡的临床特征和结局存在显著差异。

{"title":"Moxifloxacin-resistant and moxifloxacin-susceptible Staphylococcus aureus Keratitis: Outcomes from a 10-year retrospective study","authors":"Raven Diacou , Rohan Bir Singh , Eric G. Romanowski , Jonathan B. Mandell , Alex Mammen , Robert M.Q. Shanks , Vishal Jhanji","doi":"10.1016/j.jtos.2025.06.008","DOIUrl":"10.1016/j.jtos.2025.06.008","url":null,"abstract":"<div><h3>Purpose</h3><div>Fluoroquinolones, specifically moxifloxacin are commonly used to treat bacterial keratitis. However, recent clinical evidence assessing the clinical outcomes in bacterial keratitis cases resistant to moxifloxacin remains sparse. This study evaluates the clinical characteristics and outcomes in cases of <em>Staphylococcus aureus</em> keratitis with <em>in vitro</em> resistance to moxifloxacin.</div></div><div><h3>Design</h3><div>Retrospective clinical cohort study.</div></div><div><h3>Methods</h3><div>Keratitis patients with cultures positive for <em>Staphylococcus aureus</em> at the University of Pittsburgh Medical Center, were identified between July 2012 and June 2022.</div></div><div><h3>Results</h3><div>A total of 104 patients with culture-confirmed <em>Staphylococcus aureus</em> keratitis were included in the study. Patients infected by moxifloxacin-resistant bacteria (n = 32) were significantly older (74.32 ± 17.41 years) than moxifloxacin-susceptible infections (55.56 ± 20.86 years, p < 0.0001). Moxifloxacin resistance was identified in 29.8 % of cases, including methicillin-susceptible (4.8 %) and methicillin-resistant <em>Staphylococcus aureus</em> (25.96 %) isolates. The most common risk factors for moxifloxacin resistance were ocular surface disease (35.5 %) and history of prior infection (32.2 %). Moxifloxacin-resistant <em>Staphylococcus aureus</em> was associated with larger epithelial defects (18.18 ± 4.93 mm<sup>2</sup> vs. 5.10 ± 0.76 mm<sup>2</sup>, p < 0.0001), longer healing times (42.42 ± 6.75 days vs. 32.2 ± 3.56 days, p < 0.0001), and higher rates of corneal perforation (19.3 % vs. 4.2 %; p = 0.0317). Visual acuity outcomes were significantly worse in the resistant group, with minimal improvement from baseline (1.87 ± 0.14 LogMAR) to final follow-up (1.8 ± 0.18 LogMAR), compared to the moxifloxacin-susceptible group (from 1.46 ± 0.11 LogMAR to 1.15 ± 0.11 LogMAR, p < 0.0001). Enucleation was required in 9.6 % of resistant cases.</div></div><div><h3>Conclusions</h3><div>The current study determined significant differences in clinical characteristics and outcomes among corneal ulcers caused by moxifloxacin-resistant <em>Staphylococcus aureus</em>.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"38 ","pages":"Pages 97-103"},"PeriodicalIF":5.9,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Models for Meibomian gland dysfunction: In vivo and in vitro”, Review article [Ocul. Surf. 32 (2024) 154–165] “睑板腺功能障碍模型：体内和体外”的勘误表。综述文章，眼表32(2024)154-165。

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-06-23 DOI: 10.1016/j.jtos.2025.06.009

Jinghua Bu , Yuli Guo , Yang Wu , Rongrong Zhang , Jingbin Zhuang , Jiankai Zhao , Le Sun , Andrew J. Quantock , Zuguo Liu , Wei Li

引用次数: 0

Ultrasonography assessments of meibomian gland as a noninvasive method of detecting changes in morphology 超声评估睑板腺作为一种无创检测形态学变化的方法

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-06-20 DOI: 10.1016/j.jtos.2025.06.006

Liyun Wang , Hongbo Yin , Yujia Yang , Li Qiu

Purpose

We employed 24 MHz ultrasonography to evaluate the morphological features of meibomian glands (MGs) and compared the ultrasonic differences in morphology between healthy volunteers and meibomian gland dysfunction (MGD) patients.

Methods

24 MHz ultrasound examinations on both the upper and lower eyelids of healthy volunteers and patients with MGD, using transverse and longitudinal sections. The reproducibility of ultrasonographic findings for the MG was evaluated, and a comparison of morphological characteristics at different examination sites between the two groups was made.

Results

Among 89 participants, 24 were healthy volunteers and 65 had MGD. The cross-sectional ultrasound findings showed well-organized punctate hyperechoic ducts within the tarsal plate, surrounded by hypoechoic acinar formations, which were further classified based on the extent of MG involvement into three categories: normal, ≤50 % involvement, and >50 % involvement. Additionally, a typical longitudinal ultrasound appearance of a MG consisted of a thin band-like hypoechoic structure (acinar) with uniform thickness accompanied by an arc-shaped linear hyperechoic duct in its middle region. Furthermore, seven abnormal longitudinal ultrasound manifestations were categorized as distortion, ectasia, central duct truncation, cyst formation, structureless appearance, complete disappearance or other findings. The intra- and inter-observer agreement for those categories was found to be high (ICC>0.6). MGD patients exhibited a higher likelihood of overall MG involvement or abnormal ultrasound findings, particularly in relation to the middle part of the upper eyelid.

Conclusion

The present study has developed an initial classification method to identify abnormalities in MG morphology using 24 MHz ultrasound in MGD patients.

采用24 MHz超声对睑板腺（mg）的形态学特征进行了评价，并比较了健康志愿者与睑板腺功能障碍（MGD）患者的超声形态学差异。

{"title":"Ultrasonography assessments of meibomian gland as a noninvasive method of detecting changes in morphology","authors":"Liyun Wang , Hongbo Yin , Yujia Yang , Li Qiu","doi":"10.1016/j.jtos.2025.06.006","DOIUrl":"10.1016/j.jtos.2025.06.006","url":null,"abstract":"<div><h3>Purpose</h3><div>We employed 24 MHz ultrasonography to evaluate the morphological features of meibomian glands (MGs) and compared the ultrasonic differences in morphology between healthy volunteers and meibomian gland dysfunction (MGD) patients.</div></div><div><h3>Methods</h3><div>24 MHz ultrasound examinations on both the upper and lower eyelids of healthy volunteers and patients with MGD, using transverse and longitudinal sections. The reproducibility of ultrasonographic findings for the MG was evaluated, and a comparison of morphological characteristics at different examination sites between the two groups was made.</div></div><div><h3>Results</h3><div>Among 89 participants, 24 were healthy volunteers and 65 had MGD. The cross-sectional ultrasound findings showed well-organized punctate hyperechoic ducts within the tarsal plate, surrounded by hypoechoic acinar formations, which were further classified based on the extent of MG involvement into three categories: normal, ≤50 % involvement, and >50 % involvement. Additionally, a typical longitudinal ultrasound appearance of a MG consisted of a thin band-like hypoechoic structure (acinar) with uniform thickness accompanied by an arc-shaped linear hyperechoic duct in its middle region. Furthermore, seven abnormal longitudinal ultrasound manifestations were categorized as distortion, ectasia, central duct truncation, cyst formation, structureless appearance, complete disappearance or other findings. The intra- and inter-observer agreement for those categories was found to be high (ICC>0.6). MGD patients exhibited a higher likelihood of overall MG involvement or abnormal ultrasound findings, particularly in relation to the middle part of the upper eyelid.</div></div><div><h3>Conclusion</h3><div>The present study has developed an initial classification method to identify abnormalities in MG morphology using 24 MHz ultrasound in MGD patients.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"38 ","pages":"Pages 72-79"},"PeriodicalIF":5.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ocular surface epithelial immune cells and corneal nerves in dry eye disease - A systematic review and meta-analysis of in vivo confocal microscopy data 干眼病的眼表上皮免疫细胞和角膜神经-体内共聚焦显微镜数据的系统回顾和荟萃分析

IF 5.9 1区医学 Q1 OPHTHALMOLOGY

Ocular Surface

Pub Date : 2025-06-20 DOI: 10.1016/j.jtos.2025.06.005

Soumen Sadhu , Isabelle Jalbert , Luz Palacios-Derflingher , Ali Alghamdi , Blanka Golebiowski , Fiona Stapleton

Purpose

To examine ocular surface epithelial immune cell (EIC) density and corneal nerve parameters between patients with dry eye disease (DED) compared to healthy controls with further comparisons between auto-immune - Sjӧgren Syndrome DED (SS-DED) and non-autoimmune – non-Sjӧgren Syndrome DED (NSS-DED) subtypes.

Methods

A systematic review and meta-analysis following PRISMA guidelines (Prospero-CRD42023446763) were conducted including studies reporting corneal and conjunctival EIC density and/or corneal nerve parameters in DED and healthy controls using in vivo confocal microscopy. Electronic databases – PubMed, Embase, Scopus, Web of Science were searched from inception to March 2025.

Results

The meta-analysis included 24 studies (n = 1060 DED, n = 521 controls) reporting EIC density, 30 studies reporting nerve length (CNL; n = 1450 DED, n = 643 controls) and 20 studies reporting nerve density (CND; n = 919 DED, n = 462 controls) in the central cornea. Studies of other ocular surface locations were insufficient for meta-analysis. Higher central corneal EIC density was evident in DED compared to controls (MD: 57.9 cells/mm²; 95 % CI: 43.3, 72.5; p < 0.001), and in, SS-DED compared to NSS-DED (51.0 cells/mm²; 95 % CI: 12.0, 90.0; p = 0.01). DED had lower CNL (−4.0 mm/mm²; 95 % CI: 5.2, −2.7; p < 0.001) and CND (−7.2 nerves/mm²; 95 % CI: 10.3, −4.1; p < 0.001) compared to controls. No significant differences in nerve parameters were found between SS-DED and NSS-DED subtypes (CNL: 95 % CI: 3.0, 1.4, CND: 6.8, 4.8; p ≥ 0.46).

Conclusion

DED involves substantial EIC infiltration and reduced corneal nerve parameters. SS-DED is distinguished by higher EIC density than NSS-DED, while no differences were observed in corneal nerve parameters.

研究干眼病（DED）患者与健康对照组相比眼表面上皮免疫细胞（EIC）密度和角膜神经参数，并进一步比较自身免疫-Sjӧgren综合征DED （SS-DED）和非自身免疫- non-Sjӧgren综合征DED （NSS-DED）亚型之间的差异。

{"title":"Ocular surface epithelial immune cells and corneal nerves in dry eye disease - A systematic review and meta-analysis of in vivo confocal microscopy data","authors":"Soumen Sadhu , Isabelle Jalbert , Luz Palacios-Derflingher , Ali Alghamdi , Blanka Golebiowski , Fiona Stapleton","doi":"10.1016/j.jtos.2025.06.005","DOIUrl":"10.1016/j.jtos.2025.06.005","url":null,"abstract":"<div><h3>Purpose</h3><div>To examine ocular surface epithelial immune cell (EIC) density and corneal nerve parameters between patients with dry eye disease (DED) compared to healthy controls with further comparisons between auto-immune - Sjӧgren Syndrome DED (SS-DED) and non-autoimmune – non-Sjӧgren Syndrome DED (NSS-DED) subtypes.</div></div><div><h3>Methods</h3><div>A systematic review and meta-analysis following PRISMA guidelines (Prospero-CRD42023446763) were conducted including studies reporting corneal and conjunctival EIC density and/or corneal nerve parameters in DED and healthy controls using <em>in vivo</em> confocal microscopy. Electronic databases – PubMed, Embase, Scopus, Web of Science were searched from inception to March 2025.</div></div><div><h3>Results</h3><div>The meta-analysis included 24 studies (n = 1060 DED, n = 521 controls) reporting EIC density, 30 studies reporting nerve length (CNL; n = 1450 DED, n = 643 controls) and 20 studies reporting nerve density (CND; n = 919 DED, n = 462 controls) in the central cornea. Studies of other ocular surface locations were insufficient for meta-analysis. Higher central corneal EIC density was evident in DED compared to controls (MD: 57.9 cells/mm<sup>2</sup>; 95 % CI: 43.3, 72.5; p < 0.001), and in, SS-DED compared to NSS-DED (51.0 cells/mm<sup>2</sup>; 95 % CI: 12.0, 90.0; p = 0.01). DED had lower CNL (−4.0 mm/mm<sup>2</sup>; 95 % CI: 5.2, −2.7; p < 0.001) and CND (−7.2 nerves/mm<sup>2</sup>; 95 % CI: 10.3, −4.1; p < 0.001) compared to controls. No significant differences in nerve parameters were found between SS-DED and NSS-DED subtypes (CNL: 95 % CI: 3.0, 1.4, CND: 6.8, 4.8; p ≥ 0.46).</div></div><div><h3>Conclusion</h3><div>DED involves substantial EIC infiltration and reduced corneal nerve parameters. SS-DED is distinguished by higher EIC density than NSS-DED, while no differences were observed in corneal nerve parameters.</div></div>","PeriodicalId":54691,"journal":{"name":"Ocular Surface","volume":"38 ","pages":"Pages 80-96"},"PeriodicalIF":5.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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