C. Couillard, D. Maltais, Benoît Bruneau, N. Asselin, S. Boudreau
Simple nutritional condition indicators are needed to provide information on the influence of ecosystem changes on the populations of the American lobster Homarus americanus (H. Milne Edwards, 1837) on various timescales. This study validates the use of the water content (% wet mass) of the digestive gland as an indicator of nutritional condition in the lobster (carapace length 65–127 mm) by assessing its capacity to estimate digestive-gland lipid reserves under variable environmental and physiological conditions. The validation was completed using samples from wild lobsters dissected shortly after being captured during different seasons and in various locations in the St. Lawrence Estuary and Gulf of St. Lawrence (GSL), and data from an environmentally realistic laboratory study on post-ovigerous females from the southern and the northern GSL sampled at different stages of their molt cycle. In both wild and experimental lobsters, water content was the best predictor of lipid reserves compared to other condition indicators (i.e., condition factor, various digestive-gland indices, and hemolymph Brix index). A strong linear relationship was found between lipid and water contents. Lipid content and interrelated molting status were identified as two important factors leading to the variations in water-fat regression equations among groups of wild or experimental lobsters. As lipid content could vary spatiotemporally in unexpected ways in a changing environment, it is recommended to use a sampling event-specific regression line to estimate digestive-gland lipid content from measured water content in field monitoring programs. Combining water content with molt status indicators such as Brix index is recommended to support interpretation of the observed variations in condition.
{"title":"Validation of the water content of the digestive gland as an indicator of nutritional condition in the American lobster Homarus americanus (H. Milne Edwards, 1837) (Decapoda: Nephropidae)","authors":"C. Couillard, D. Maltais, Benoît Bruneau, N. Asselin, S. Boudreau","doi":"10.1093/jcbiol/ruad016","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad016","url":null,"abstract":"\u0000 Simple nutritional condition indicators are needed to provide information on the influence of ecosystem changes on the populations of the American lobster Homarus americanus (H. Milne Edwards, 1837) on various timescales. This study validates the use of the water content (% wet mass) of the digestive gland as an indicator of nutritional condition in the lobster (carapace length 65–127 mm) by assessing its capacity to estimate digestive-gland lipid reserves under variable environmental and physiological conditions. The validation was completed using samples from wild lobsters dissected shortly after being captured during different seasons and in various locations in the St. Lawrence Estuary and Gulf of St. Lawrence (GSL), and data from an environmentally realistic laboratory study on post-ovigerous females from the southern and the northern GSL sampled at different stages of their molt cycle. In both wild and experimental lobsters, water content was the best predictor of lipid reserves compared to other condition indicators (i.e., condition factor, various digestive-gland indices, and hemolymph Brix index). A strong linear relationship was found between lipid and water contents. Lipid content and interrelated molting status were identified as two important factors leading to the variations in water-fat regression equations among groups of wild or experimental lobsters. As lipid content could vary spatiotemporally in unexpected ways in a changing environment, it is recommended to use a sampling event-specific regression line to estimate digestive-gland lipid content from measured water content in field monitoring programs. Combining water content with molt status indicators such as Brix index is recommended to support interpretation of the observed variations in condition.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49124819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gayathri Ambatt Padmanabhan, R. Chakraborty, Sreesanth Lakshman
Diet composition and feeding strategies of deep-sea caridean shrimp Heterocarpus woodmasoniAlcock,1901 are described from gut contents of 4,116 specimens collected from the southwestern coast of India. Knowledge of the species diet plays a prime effect on its biological aspects and its role in the ecological energetics of the community. All the analyses were performed in accordance with ecological (season) and biological factors (sex, size, and gonadal stages). The species was found to prey on diversified components such as detritus, foraminiferans, other crustaceans, molluscs, echinoderms, and fishes in their declining order of preponderance. Multivariate analysis of variance showed a significant difference (P<0.05) in the diet components according to the combination of sex along with season and gonadal stages. Values of the gastro-somatic index (GSI) showed a positive correlation with feeding activity, and its intensity. GSI, feeding intensity, and activity reached their peak values during the post-monsoon season. High vacuity index was recorded in almost all conditions, which is a highlighted feature of this species. These observations provide fundamental information on the feeding pattern of H. woodmasoni, which aims to develop suitable fishing strategies in the future.
{"title":"Food and feeding strategies of the deep-sea shrimp Heterocarpus woodmasoni, Alcock 1901 (Decapoda: Pandalidae) from southwestern India","authors":"Gayathri Ambatt Padmanabhan, R. Chakraborty, Sreesanth Lakshman","doi":"10.1093/jcbiol/ruad005","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad005","url":null,"abstract":"\u0000 Diet composition and feeding strategies of deep-sea caridean shrimp Heterocarpus woodmasoniAlcock,1901 are described from gut contents of 4,116 specimens collected from the southwestern coast of India. Knowledge of the species diet plays a prime effect on its biological aspects and its role in the ecological energetics of the community. All the analyses were performed in accordance with ecological (season) and biological factors (sex, size, and gonadal stages). The species was found to prey on diversified components such as detritus, foraminiferans, other crustaceans, molluscs, echinoderms, and fishes in their declining order of preponderance. Multivariate analysis of variance showed a significant difference (P<0.05) in the diet components according to the combination of sex along with season and gonadal stages. Values of the gastro-somatic index (GSI) showed a positive correlation with feeding activity, and its intensity. GSI, feeding intensity, and activity reached their peak values during the post-monsoon season. High vacuity index was recorded in almost all conditions, which is a highlighted feature of this species. These observations provide fundamental information on the feeding pattern of H. woodmasoni, which aims to develop suitable fishing strategies in the future.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45989007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal Article News from The Crustacean Society for March 2023, Vol. 43, No. 1 Get access Journal of Crustacean Biology, Volume 43, Issue 1, March 2023, ruad015, https://doi.org/10.1093/jcbiol/ruad015 Published: 24 March 2023
{"title":"News from The Crustacean Society for March 2023, Vol. 43, No. 1","authors":"","doi":"10.1093/jcbiol/ruad015","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad015","url":null,"abstract":"Journal Article News from The Crustacean Society for March 2023, Vol. 43, No. 1 Get access Journal of Crustacean Biology, Volume 43, Issue 1, March 2023, ruad015, https://doi.org/10.1093/jcbiol/ruad015 Published: 24 March 2023","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":"159 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135185303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Asem, Chaojie Yang, A. Eimanifar, F. Hontoria, I. Varó, Farnaz Mahmoudi, Chun-Zheng Fu, Chun-Yang Shen, N. Rastegar-Pouyani, Peizheng Wang, Weidong Li, Liping Yao, Xinyu Meng, Y. Dan, D. Rogers, G. Gajardo
Species of Artemia are regionally endemic branchiopod crustaceans composed of sexual species and parthenogenetic lineages, and represent an excellent model for studying adaptation and speciation to extreme and heterogeneous hypersaline environments. We tested hypotheses of whether populations from the Tibetan Plateau belong to A. tibetianaAbatzopoulos, Zhang & Sorgeloos,1998 and whether a population from Kazakhstan is a new species, using other Asian species of Artemia as outgroups. We conducted a multitrait phylogenetic study based on the complete mitogenome, mitochondrial (COI, 12S, 16S) and nuclear (microsatellites, ITS1) markers, and a suit of uni- and multivariate morphological traits. Our results led to the discovery of two new species, one from the Tibetan Plateau (Haiyan Lake) in China (Artemia sorgeloosin. sp.) and a second from Kazakhstan (Artemia amatin. sp.). Our analysis demonstrate that A. tibetiana and A. amatin. sp. are monophyletic, whereas A. sorgeloosin. sp., and A. tibetiana are polyphyletic. Evolutionary relationships based on mitochondrial and nSSR markers suggest that A. tibetiana may have arisen from a past hybridization event of a maternal ancestor of A. tibetiana with A. sorgeloosin. sp. or its ancestor. We present the complete mitogenome of A. tibetiana, A. amatin. sp., and A. sorgeloosin. sp. We also provide a novel taxonomic identification key based on morphology, emphasizing the phenotype as a necessary component of the species concept.
{"title":"Phylogenetic analysis of problematic Asian species of Artemia Leach, 1819 (Crustacea, Anostraca), with the descriptions of two new species","authors":"A. Asem, Chaojie Yang, A. Eimanifar, F. Hontoria, I. Varó, Farnaz Mahmoudi, Chun-Zheng Fu, Chun-Yang Shen, N. Rastegar-Pouyani, Peizheng Wang, Weidong Li, Liping Yao, Xinyu Meng, Y. Dan, D. Rogers, G. Gajardo","doi":"10.1093/jcbiol/ruad002","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad002","url":null,"abstract":"\u0000 Species of Artemia are regionally endemic branchiopod crustaceans composed of sexual species and parthenogenetic lineages, and represent an excellent model for studying adaptation and speciation to extreme and heterogeneous hypersaline environments. We tested hypotheses of whether populations from the Tibetan Plateau belong to A. tibetianaAbatzopoulos, Zhang & Sorgeloos,1998 and whether a population from Kazakhstan is a new species, using other Asian species of Artemia as outgroups. We conducted a multitrait phylogenetic study based on the complete mitogenome, mitochondrial (COI, 12S, 16S) and nuclear (microsatellites, ITS1) markers, and a suit of uni- and multivariate morphological traits. Our results led to the discovery of two new species, one from the Tibetan Plateau (Haiyan Lake) in China (Artemia sorgeloosin. sp.) and a second from Kazakhstan (Artemia amatin. sp.). Our analysis demonstrate that A. tibetiana and A. amatin. sp. are monophyletic, whereas A. sorgeloosin. sp., and A. tibetiana are polyphyletic. Evolutionary relationships based on mitochondrial and nSSR markers suggest that A. tibetiana may have arisen from a past hybridization event of a maternal ancestor of A. tibetiana with A. sorgeloosin. sp. or its ancestor. We present the complete mitogenome of A. tibetiana, A. amatin. sp., and A. sorgeloosin. sp. We also provide a novel taxonomic identification key based on morphology, emphasizing the phenotype as a necessary component of the species concept.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43319074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Victor Tosin Okomoda, Jung Liew Hon, Mazelan Suhairi, Sabri Muda Mohd, Ibrahim Zaidi, Rezuan Rusdi Iffa, Othman Rohisyamuddin, Hayati Ismail Nurul, Afis Gusti Roslan Gusti, Dahlianis Ishak Sairatul, Najmi Ishak Ahmad, Azman Kasan Nor, Liang Liqun, Chang Yumei, Mhd Abdullah Ikhwanuddin
Abstract This study was conducted to evaluate the morphological variation of Macrobrachium rosenbergii (De Man, 1879) from the Nyatoh River, Terengganu state, Malaysia. Three hundred and thirty-five samples of M. rosenbergii comprising 41.5% males and 58.5% females were collected from August 2019 to October 2020. The bulk of the sample collection had small claws (69.1%), whereas those with orange and blue claws constituted 19.1% and 11.8%, respectively. The result obtained from the analysis of nine sets of morphometric data for both sexes showed univariate morphometric similarities and wide overlap in principal component analysis (PCA) without separation into unique clusters. Comparison by claw-type showed significant variation in the univariate analysis of the morphometric parameters measured in the following order: blue claw > orange claw > small claw. PCA did not cluster the prawns into different groups, hence multivariate analysis of morphometric parameters cannot be used to distinguish the prawn by claw type should the appendages be lost. Comparisons of the length-weight relationship data and condition factor suggest that the Terengganu River prawn population had a positive allometric growth pattern and were in good condition. The Pearson’s correlation analysis also showed a largely positive relationship between the morphometric data collected (with a few exceptions in the different morphotypes). The morphological measurements could not therefore be used to discriminate the sex and claw-type of freshwater prawn M. rosenbergii in the Nyatoh River.
{"title":"Morphometric and sexual characteristics of<i>Macrobrachium rosenbergii</i>(De Man, 1879) from the Nyatoh River, Malaysia","authors":"Victor Tosin Okomoda, Jung Liew Hon, Mazelan Suhairi, Sabri Muda Mohd, Ibrahim Zaidi, Rezuan Rusdi Iffa, Othman Rohisyamuddin, Hayati Ismail Nurul, Afis Gusti Roslan Gusti, Dahlianis Ishak Sairatul, Najmi Ishak Ahmad, Azman Kasan Nor, Liang Liqun, Chang Yumei, Mhd Abdullah Ikhwanuddin","doi":"10.1093/jcbiol/ruad009","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad009","url":null,"abstract":"Abstract This study was conducted to evaluate the morphological variation of Macrobrachium rosenbergii (De Man, 1879) from the Nyatoh River, Terengganu state, Malaysia. Three hundred and thirty-five samples of M. rosenbergii comprising 41.5% males and 58.5% females were collected from August 2019 to October 2020. The bulk of the sample collection had small claws (69.1%), whereas those with orange and blue claws constituted 19.1% and 11.8%, respectively. The result obtained from the analysis of nine sets of morphometric data for both sexes showed univariate morphometric similarities and wide overlap in principal component analysis (PCA) without separation into unique clusters. Comparison by claw-type showed significant variation in the univariate analysis of the morphometric parameters measured in the following order: blue claw &gt; orange claw &gt; small claw. PCA did not cluster the prawns into different groups, hence multivariate analysis of morphometric parameters cannot be used to distinguish the prawn by claw type should the appendages be lost. Comparisons of the length-weight relationship data and condition factor suggest that the Terengganu River prawn population had a positive allometric growth pattern and were in good condition. The Pearson’s correlation analysis also showed a largely positive relationship between the morphometric data collected (with a few exceptions in the different morphotypes). The morphological measurements could not therefore be used to discriminate the sex and claw-type of freshwater prawn M. rosenbergii in the Nyatoh River.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135185650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jason D Williams, Christopher B Boyko, Carolyn K Tepolt, April M H Blakeslee
Abstract Although intertidal crabs from the western Atlantic coast are relatively well studied for some parasite fauna, their parasitic isopods are poorly known. We document two endoparasitic entoniscids (Cancrion carolinusPearse & Walker, 1939 and Cryptocancrion brevibrachiumn. gen., n. sp.) from mud (Brachyura: Panopeidae) and stone (Brachyura: Menippidae) crabs based on molecular and morphological data, including descriptions of their epicaridium larvae. Although these species cannot be distinguished based on adult female characters, molecular data and larval and male morphologies show that two species are present, sometimes sympatrically. The only other species of CancrionGiard & Bonnier, 1887 from the Atlantic coast of North America is Cancrion needleriPearse & Walker, 1939, described from adult specimens parasitizing Dyspanopeus sayi (Smith, 1869) from Prince Edward Island, Canada. Larvae of this species were never described and we consider it a species inquirenda. Cryptocancrion brevibrachiumn. gen., n. sp. is documented to have epicaridium larvae with a “short-armed” morphology (sixth pereopod subequal in length to pereopods 1–5), and the adults parasitize at least three mud crabs: D. sayi, Panopeus herbstiiH. Milne Edwards, 1834, and Rhithropanopeus harrisii (Gould, 1841). By contrast, Cancrion carolinus (originally described from P. herbstii collected in Beaufort, NC, USA) has epicaridium larvae with a “long-armed” morphology (sixth pereopod approximately twice the length of pereopods 1–5) and is found in at least four host species: Eurypanopeus depressus (Smith, 1869), P. herbstii, and R. harrisii and the stone crab Menippe mercenaria (Say, 1818). We describe C. brevibrachiumn. gen., n. sp. and redescribe C. carolinus based on type material and fresh specimens. The cryptic nature of these entoniscids indicates that examination of epicaridium larvae and/or molecular data are needed to properly identify species.
{"title":"Cryptic diversity in endoparasitic isopods (Bopyroidea: Entoniscidae) from mud crabs (Panopeidae) along the Atlantic coast of North America, with the description of a new genus and new species as revealed by molecular and larval characters: the long and the short of it","authors":"Jason D Williams, Christopher B Boyko, Carolyn K Tepolt, April M H Blakeslee","doi":"10.1093/jcbiol/ruac065","DOIUrl":"https://doi.org/10.1093/jcbiol/ruac065","url":null,"abstract":"Abstract Although intertidal crabs from the western Atlantic coast are relatively well studied for some parasite fauna, their parasitic isopods are poorly known. We document two endoparasitic entoniscids (Cancrion carolinusPearse & Walker, 1939 and Cryptocancrion brevibrachiumn. gen., n. sp.) from mud (Brachyura: Panopeidae) and stone (Brachyura: Menippidae) crabs based on molecular and morphological data, including descriptions of their epicaridium larvae. Although these species cannot be distinguished based on adult female characters, molecular data and larval and male morphologies show that two species are present, sometimes sympatrically. The only other species of CancrionGiard & Bonnier, 1887 from the Atlantic coast of North America is Cancrion needleriPearse & Walker, 1939, described from adult specimens parasitizing Dyspanopeus sayi (Smith, 1869) from Prince Edward Island, Canada. Larvae of this species were never described and we consider it a species inquirenda. Cryptocancrion brevibrachiumn. gen., n. sp. is documented to have epicaridium larvae with a “short-armed” morphology (sixth pereopod subequal in length to pereopods 1–5), and the adults parasitize at least three mud crabs: D. sayi, Panopeus herbstiiH. Milne Edwards, 1834, and Rhithropanopeus harrisii (Gould, 1841). By contrast, Cancrion carolinus (originally described from P. herbstii collected in Beaufort, NC, USA) has epicaridium larvae with a “long-armed” morphology (sixth pereopod approximately twice the length of pereopods 1–5) and is found in at least four host species: Eurypanopeus depressus (Smith, 1869), P. herbstii, and R. harrisii and the stone crab Menippe mercenaria (Say, 1818). We describe C. brevibrachiumn. gen., n. sp. and redescribe C. carolinus based on type material and fresh specimens. The cryptic nature of these entoniscids indicates that examination of epicaridium larvae and/or molecular data are needed to properly identify species.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136095722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chelonibia testudinaria (Linnaeus, 1758) is a globally distributed epibiont barnacle using different hosts, including sea turtles and portunid crabs. Previous phylogenetic analyses have recovered three distinct clades for this species corresponding to the Atlantic, Eastern Pacific, and Indo-West Pacific regions. The present study of the genetic diversity of this species in the Persian Gulf (northwestern Indian Ocean) using the COX1 mitochondrial marker estimates the genetic connectivity of the barnacle between the Persian Gulf and two western Pacific regions. No significant genetic differentiation is evident between the Persian Gulf and the western Pacific, but the moderate level of genetic connectivity found between these areas across the Indian Ocean is likely mediated by migratory sea turtles. Based on the present analysis, the Persian Gulf displays a lower genetic diversity than the diversity of the western Pacific populations but shares some haplotypes with the latter.
{"title":"Gene flow with the help of sea turtles: phylogeography of the epibiont barnacle Chelonibia testudinaria (Linnaeus, 1758) (Crustacea: Cirripedia: Chelonibiidae) from the Persian Gulf","authors":"A. Shahdadi","doi":"10.1093/jcbiol/ruad014","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad014","url":null,"abstract":"\u0000 Chelonibia testudinaria (Linnaeus, 1758) is a globally distributed epibiont barnacle using different hosts, including sea turtles and portunid crabs. Previous phylogenetic analyses have recovered three distinct clades for this species corresponding to the Atlantic, Eastern Pacific, and Indo-West Pacific regions. The present study of the genetic diversity of this species in the Persian Gulf (northwestern Indian Ocean) using the COX1 mitochondrial marker estimates the genetic connectivity of the barnacle between the Persian Gulf and two western Pacific regions. No significant genetic differentiation is evident between the Persian Gulf and the western Pacific, but the moderate level of genetic connectivity found between these areas across the Indian Ocean is likely mediated by migratory sea turtles. Based on the present analysis, the Persian Gulf displays a lower genetic diversity than the diversity of the western Pacific populations but shares some haplotypes with the latter.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49579883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Crustaceans inhabiting freshwater are more vulnerable to increased loading with dissolved trace metals than those from more saline conditions for both physicochemical and physiological reasons. Potamonautes warreni (Calman, 1918) is a true freshwater crab inhabiting rivers in proximity to mining activities in South Africa. In order to assess whether the uptake of zinc (Zn2+) and cadmium (Cd2+) were associated with Na+- and Ca2+-uptake routes, individuals of P. warreni were acclimated to different Na+ or Ca2+ concentrations before measuring trace-metal uptake into the haemolymph and tissues in an intermediate freshwater. Cd2+ uptake into the haemolymph was elevated following acclimation to ‘Na+ replete’ conditions, when compared with ‘Na+ deplete’ conditions, indicating that a physiological response to the acclimation salinity had influenced Cd2+ uptake. Zn2+ uptake by the whole body was elevated in P. warreni individuals from the ‘Na+ replete’ group when compared with crabs acclimated to Na+ free water. A general change in permeability may have occurred and indeed, the apparent water permeability (water efflux) of P. warreni was increased in elevated Na+ and Ca2+ concentrations compared with that in softer water. A comparison of the osmoregulatory ability between P. warreni and the catadromous crab Eriocheir sinensisH. Milne Edwards, 1853 highlighted the greater permeability of the latter. Lower Zn2+ and Cd2+ uptake rates were measured in P. warreni in comparison to those measured in E. sinensis by other authors, indicating that the more well adapted a species is to life in freshwater, the less susceptible they will be to metal uptake. Future investigations to understand further the mechanisms influencing Zn2+ and Cd2+ uptake are required to understand the role that salinity can play in protecting a species from trace-metals released into the environment.
{"title":"Is salt the solution? Interactions between trace-metal uptake and salinity in the freshwater crab Potamonautes warreni Calman, 1918 (Decapoda: Brachyura: Potamonautidae)","authors":"Susan Glendinning, A. Vosloo","doi":"10.1093/jcbiol/ruad010","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad010","url":null,"abstract":"\u0000 Crustaceans inhabiting freshwater are more vulnerable to increased loading with dissolved trace metals than those from more saline conditions for both physicochemical and physiological reasons. Potamonautes warreni (Calman, 1918) is a true freshwater crab inhabiting rivers in proximity to mining activities in South Africa. In order to assess whether the uptake of zinc (Zn2+) and cadmium (Cd2+) were associated with Na+- and Ca2+-uptake routes, individuals of P. warreni were acclimated to different Na+ or Ca2+ concentrations before measuring trace-metal uptake into the haemolymph and tissues in an intermediate freshwater. Cd2+ uptake into the haemolymph was elevated following acclimation to ‘Na+ replete’ conditions, when compared with ‘Na+ deplete’ conditions, indicating that a physiological response to the acclimation salinity had influenced Cd2+ uptake. Zn2+ uptake by the whole body was elevated in P. warreni individuals from the ‘Na+ replete’ group when compared with crabs acclimated to Na+ free water. A general change in permeability may have occurred and indeed, the apparent water permeability (water efflux) of P. warreni was increased in elevated Na+ and Ca2+ concentrations compared with that in softer water. A comparison of the osmoregulatory ability between P. warreni and the catadromous crab Eriocheir sinensisH. Milne Edwards, 1853 highlighted the greater permeability of the latter. Lower Zn2+ and Cd2+ uptake rates were measured in P. warreni in comparison to those measured in E. sinensis by other authors, indicating that the more well adapted a species is to life in freshwater, the less susceptible they will be to metal uptake. Future investigations to understand further the mechanisms influencing Zn2+ and Cd2+ uptake are required to understand the role that salinity can play in protecting a species from trace-metals released into the environment.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43097009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qianqian Wang, C. Cai, Junbiao Zhang, Dianzhang Shui, Guangtong Cui, Zhi Wang, Shengjie Ren, Ping Wu, Yuantu Ye
Hormone-sensitive lipase (HSL) is an important regulator of cellular lipid homeostasis and catalyzes the hydrolysis of stored triacylglycerol. We identified and cloned for the first time the full-length cDNA sequence of HSL of the prawn Macrobrachium nipponense De Haan, 1849 [De Haan, 1833–1850] from a hepatopancreas cDNA library. The complete HSL sequence is 3,575 bp and encoded a 785 amino acid peptide with the catalytic core (GXSXG) containing a serine residue. The phylogenetic tree revealed that the gene of HSL of M. nipponense is closely related with that of Penaeus vanmameiBoone, 1931. The tissue distribution showed that the mRNA expression level of HSL in the hepatopancreas was significantly higher than that in other tissues (P < 0.05). Furthermore, the HSL expression in hepatopancreas was upregulated with the increase of dietary lipids but partially inhibited when the ratio of phospholipids was increased in the lipid mixture. These results demonstrate that HSL is involved in the lipid metabolism of M. nipponense and highlights the importance of phospholipids in lipid metabolism.
{"title":"Gene cloning, expression pattern, and response to dietary total lipids and phospholipids of hormone-sensitive lipase (HSL) in the Oriental river prawn Macrobrachium nipponense De Haan, 1849 (Decapoda: Caridea: Palaemonidae)","authors":"Qianqian Wang, C. Cai, Junbiao Zhang, Dianzhang Shui, Guangtong Cui, Zhi Wang, Shengjie Ren, Ping Wu, Yuantu Ye","doi":"10.1093/jcbiol/ruad011","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad011","url":null,"abstract":"\u0000 Hormone-sensitive lipase (HSL) is an important regulator of cellular lipid homeostasis and catalyzes the hydrolysis of stored triacylglycerol. We identified and cloned for the first time the full-length cDNA sequence of HSL of the prawn Macrobrachium nipponense De Haan, 1849 [De Haan, 1833–1850] from a hepatopancreas cDNA library. The complete HSL sequence is 3,575 bp and encoded a 785 amino acid peptide with the catalytic core (GXSXG) containing a serine residue. The phylogenetic tree revealed that the gene of HSL of M. nipponense is closely related with that of Penaeus vanmameiBoone, 1931. The tissue distribution showed that the mRNA expression level of HSL in the hepatopancreas was significantly higher than that in other tissues (P < 0.05). Furthermore, the HSL expression in hepatopancreas was upregulated with the increase of dietary lipids but partially inhibited when the ratio of phospholipids was increased in the lipid mixture. These results demonstrate that HSL is involved in the lipid metabolism of M. nipponense and highlights the importance of phospholipids in lipid metabolism.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47718915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yadian Israel La Rosa-Izquierdo, Sergio Hernández-Trujillo, Ulises Amador-Marrero
Abstract Although there are quantitative studies of the effect of formalin storage on copepod body size, no comparable data exist concerning storage in ethanol. We determined the degree of shrinkage of adult specimens of Parvocalanus crassirostris (Dahl, 1894) preserved in 96% ethanol for different periods of time. Measurements of the prosome of 50 females and 50 males were undertaken in fresh specimens and 7, 15, 30, and 60 days after initial preservation in ethanol. Despite variation among specimens, no significant shrinkage (P > 0.05) was detected on average males. For females, significant shrinkage (mean –4.2%; P < 0.05) was observed after seven days, with no further significant change thereafter.
{"title":"Effect of ethanol preservation on prosomal length of the calanoid copepod <i>Parvocalanus crassirostris</i> (Dahl, 1894)","authors":"Yadian Israel La Rosa-Izquierdo, Sergio Hernández-Trujillo, Ulises Amador-Marrero","doi":"10.1093/jcbiol/ruad017","DOIUrl":"https://doi.org/10.1093/jcbiol/ruad017","url":null,"abstract":"Abstract Although there are quantitative studies of the effect of formalin storage on copepod body size, no comparable data exist concerning storage in ethanol. We determined the degree of shrinkage of adult specimens of Parvocalanus crassirostris (Dahl, 1894) preserved in 96% ethanol for different periods of time. Measurements of the prosome of 50 females and 50 males were undertaken in fresh specimens and 7, 15, 30, and 60 days after initial preservation in ethanol. Despite variation among specimens, no significant shrinkage (P &gt; 0.05) was detected on average males. For females, significant shrinkage (mean –4.2%; P &lt; 0.05) was observed after seven days, with no further significant change thereafter.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135240368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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