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Mitigation of acrylamide in fried potato chips using immersion in green tea extract 用绿茶提取物浸泡油炸薯片降低丙烯酰胺含量
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-11-06 DOI: 10.1007/s00217-025-04918-4
Laura Becker, Anika Haas, Zeynep Kaplan, Eva Hölzle, Panagiotis Steliopoulos, Thomas M. Amrein, Maurus Biedermann, Katharina A. Scherf, Claudia Oellig

This study focused on using natural plant extracts to mitigate the process contaminant acrylamide in fried potato chips, with an emphasis on industrial applicability. Extracts from various plants were tested in a model system for screening purposes. Green tea extract was found to be the most suitable extract for pre-frying treatments, because of its potential to mitigate acrylamide and its good water solubility. For two different green tea extracts, immersion at room temperature (25 °C) for a short time (1.5 min) and at a low concentration (0.1 g L–1) resulted in a decrease of acrylamide by 40% under laboratory-scale and by 28% under scaled-up conditions, while higher and lower temperatures were ineffective for acrylamide mitigation. A sensory evaluation with 26 participants confirmed the suitability of the treatment. Interestingly, using catechins present in green tea (epigallocatechin gallate and epicatechin) instead of green tea extract increased acrylamide levels, which may be due to the oxidation of these phenols. Immersion in green tea extract prior to frying is a promising approach to lower acrylamide contents in fried chips.

本研究的重点是使用天然植物提取物来减轻油炸薯片中的工艺污染物丙烯酰胺,重点是工业适用性。在模型系统中测试了各种植物的提取物以进行筛选。绿茶提取物被发现是最适合用于煎炸前处理的提取物,因为它有可能减轻丙烯酰胺的作用,而且它的水溶性很好。对于两种不同的绿茶提取物,在室温(25°C)下短时间(1.5分钟)和低浓度(0.1 g L-1)浸泡导致丙烯酰胺在实验室规模下减少40%,在放大条件下减少28%,而更高和更低的温度对丙烯酰胺的缓解无效。对26名参与者的感官评估证实了治疗的适宜性。有趣的是,使用绿茶中的儿茶素(表没食子儿茶素没食子酸酯和表儿茶素)代替绿茶提取物会增加丙烯酰胺的含量,这可能是由于这些酚类物质的氧化。在油炸之前浸泡绿茶提取物是降低油炸薯条中丙烯酰胺含量的一种很有前途的方法。
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引用次数: 0
NMR fingerprinting in apricot (Prunus Armeniaca L.) kernels and fruits concerning its different cultivars and soil type as analyzed using chemometrics 用化学计量学方法分析了不同品种和土壤类型杏(Prunus Armeniaca L.)核磁共振指纹图谱
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-31 DOI: 10.1007/s00217-025-04907-7
Shaimaa Fayez, Abdelaziz F. S. Farrag, Mohamed A. Farag

Prunus armeniaca L. (apricot) is an economical tree widely cultivated for its nutritive and health benefits. Herein, we standardized apricot fruits and kernels for the first time in context to different cultivars and soil type using 1D and 2D NMR metabolomics leading to the identification of 23 metabolites belonging to sugars, amino acids, organic, phenolic, and fatty acids, flavonoids, sterols, vitamins, cyanogenic glycosides, and carotenoids accounting for apricot nutritive and sensory attributes. Multivariate data analysis revealed distinct metabolic differences between the fruits and kernels, with the fruits being higher in sugars, organic acids, and flavonoids, whereas kernels were richer in fatty acids, phytosterols, and amygdalin. OPLS-DA plot revealed apricots from sandy soil to be high in organic acids however those from muddy soil were rich in sugars. This study underscores the influence of soil type on the chemical composition of apricot fruits and kernels, of value for standardization and quality control purposes.

杏(Prunus armeniaca L.)因其营养和保健价值而被广泛种植。在此,我们首次使用1D和2D NMR代谢组学对杏果实和果仁进行标准化,根据不同的品种和土壤类型,鉴定出23种代谢物,分别属于糖、氨基酸、有机、酚类和脂肪酸、黄酮类、甾醇、维生素、氰基苷和类胡萝卜素,这些代谢物代表了杏的营养和感官属性。多变量数据分析显示,果实和果仁之间的代谢差异明显,果实的糖、有机酸和类黄酮含量较高,而果仁的脂肪酸、植物甾醇和苦杏仁苷含量较高。OPLS-DA图显示,沙质土壤的杏子有机酸含量较高,而泥质土壤的杏子有机酸含量较高。该研究强调了土壤类型对杏果实和果仁化学成分的影响,为标准化和质量控制提供了价值。
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引用次数: 0
Sainfoin (Onobrychis viciifolia L.) protein isolate as a new source of alternative plant-based protein: cytotoxicity, immunoreactivity, nutritional and functional properties 杉木蛋白分离物作为替代植物蛋白的新来源:细胞毒性、免疫反应性、营养和功能特性
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-28 DOI: 10.1007/s00217-025-04910-y
Fatma Korkmaz, Sevde Nur Güngör, Şükrü Güleç, Fatma Betül Sakarya, Ali Emre Andaç, Neşe Yılmaz Tuncel, Gülay Özkan, Esra Capanoglu, Mehmet Çağlar Tülbek, Necati Barış Tuncel

The objective of this research was to develop an alternative plant-based protein isolate using sainfoin (Onobrychis viciifolia L.) seeds. The extraction process was optimized using response surface methodology (RSM) based on the Box-Behnken Design, which examined the effects of key parameters: solvent/solid ratio (10–50 mL/g), pH (8–11), temperature (20–50 °C), and extraction time (30–120 min), aiming to maximize protein yield. The optimal extraction conditions identified were a solvent/solid ratio of 49.96 mL/g, pH of 10.99, temperature of 20 °C, and a duration of 38.55 min, achieving a protein yield of 56.36%. Additionally, the amino acid composition, cytotoxicity, immunoreactivity, and functional properties of the sainfoin seed protein isolate (SPI) were evaluated. SPI exhibited a high crude protein content of 91.44%, with arginine being the most abundant amino acid at 158.20 mg/g. The protein isolate comprised a remarkable value of 50.26% essential amino acids. Additionally, SPI demonstrated desirable functional properties, including solubility of 53.95% at neutral pH, water holding capacity of 2.36 g/g, and oil binding capacity of 4.68 g/g. Its emulsifying performance was notable, with emulsion activity and stability values of 66.67% and 77.50%, respectively. Moreover, in vitro cell culture studies demonstrated that sainfoin seed protein exhibited no adverse effects on cellular toxicity or immunoreactivity. This study highlights the potential of SPI as a novel, high-quality plant protein source with promising nutritional and functional properties and demonstrates its potential as a functional ingredient in the formulation of plant-based foods, meat analogs, and dietary supplements.

本研究的目的是利用红豆素(Onobrychis viciifolia L.)种子开发一种可替代的植物性分离蛋白。基于Box-Behnken设计,采用响应面法(RSM)优化提取工艺,考察了液料比(10-50 mL/g)、pH(8-11)、温度(20-50℃)、提取时间(30-120 min)等关键参数对提取工艺的影响。最佳提取条件为液料比49.96 mL/g, pH为10.99,温度为20℃,提取时间为38.55 min,提取率为56.36%。此外,还对红豆素种子分离蛋白(SPI)的氨基酸组成、细胞毒性、免疫反应性和功能特性进行了评价。SPI粗蛋白质含量高达91.44%,其中精氨酸含量最高,为158.20 mg/g。分离蛋白中必需氨基酸含量高达50.26%。此外,SPI显示出理想的功能特性,包括在中性pH下的溶解度为53.95%,持水能力为2.36 g/g,油结合能力为4.68 g/g。其乳化性能显著,乳化活性和稳定性值分别为66.67%和77.50%。此外,体外细胞培养研究表明,红豆素种子蛋白对细胞毒性或免疫反应性没有不良影响。这项研究强调了SPI作为一种具有良好营养和功能特性的新型、高质量植物蛋白来源的潜力,并展示了其作为植物性食品、肉类类似物和膳食补充剂配方中的功能成分的潜力。
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引用次数: 0
Research progress on milk and egg allergens 牛奶和鸡蛋过敏原的研究进展
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-28 DOI: 10.1007/s00217-025-04923-7
Fengguang Pan, Riyi Wang, Jing Liu, Siming Tan, Xiangdan Luo

Food allergies affect approximately 220 million people worldwide, with a prevalence of 3% among adults and 10% among children, and have been on the rise over the past few decades. Among infants and young children, cow’s milk protein allergy (CMPA) and egg allergy are particularly prevalent. This review aims to synthesize current knowledge on the characterization of major milk and egg allergens and critically evaluate methods for reducing their allergenicity. Through a systematic analysis of the literature, we delineate the primary allergenic proteins in these foods and elucidate their immune recognition patterns via epitope mapping, thereby offering novel insights into the molecular mechanisms of allergic reactions. Furthermore, we assess the efficacy of diverse allergen-reduction strategies, including thermal processing, high-pressure treatment, irradiation, glycosylation, ultrasonication, enzymatic hydrolysis, and fermentation. Evidence indicates that these techniques can effectively modulate protein structure and diminish sensitizing capacity. Notably, fermentation emerges as a dual-purpose technology, not only mitigating allergenicity but also enhancing nutritional value and sensory attributes. By consolidating these findings, this review underscores their significance for the food industry and allergic consumers and proposes strategic directions for future research to refine processing technologies and alleviate the risks associated with food allergies.

食物过敏影响全球约2.2亿人,其中成人患病率为3%,儿童患病率为10%,并且在过去几十年中呈上升趋势。在婴幼儿中,牛奶蛋白过敏(CMPA)和鸡蛋过敏尤为普遍。这篇综述的目的是综合目前的知识对主要的牛奶和鸡蛋过敏原的特性和批判性评价方法,以减少其致敏性。通过对文献的系统分析,我们描述了这些食物中的主要过敏原蛋白,并通过表位定位阐明了它们的免疫识别模式,从而为过敏反应的分子机制提供了新的见解。此外,我们评估了各种过敏原减少策略的效果,包括热处理、高压处理、辐照、糖基化、超声、酶水解和发酵。有证据表明,这些技术可以有效地调节蛋白质结构,降低致敏能力。值得注意的是,发酵作为一种双重用途的技术出现,不仅可以减轻过敏原,还可以提高营养价值和感官属性。通过巩固这些发现,本综述强调了它们对食品工业和过敏消费者的重要性,并提出了未来研究的战略方向,以改进加工技术和减轻与食物过敏相关的风险。
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引用次数: 0
Heat matters: role of thermal processing on millet composition, digestibility, bioaccessibility and bioavailability 热物质:热加工对谷子组成、消化率、生物可及性和生物利用度的影响
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-25 DOI: 10.1007/s00217-025-04911-x
V. M. Malathi, Rachna Sehrawat, Dibyakanta Seth, M. Pravitha, R. Venkateswarlu, A. Subeesh, Veda Krishnan, C. Tara Satyavathi

Millets are small-grained, climate-resilient cereals that assume great significance in imparting sustainable food and nutritional security. Besides their agronomic advantages, the millets are highly nutritious and contain a wide range of phytochemicals with human health beneficial properties. There has been a recent surge in the consumption of these heritage grains and food technologists across the globe are exploring several heat-imparted processing methods for developing improved products out of these gluten-free grains. Therefore, this work aims to serve as a resource covering the state-of-the-art known information about the conventional and emerging thermal processing techniques employed in millets. However, identifying a specific conventional or emerging technique for precise improvement in nutritional or overall quality remains a distant goal, as the effects of these techniques depend on factors like dosage, duration, and other process-related variables, along with the dynamics of the food matrix. This review not only analysed the common thermal processing methods used for millets but also their influence on nutritional quality, digestibility, bio accessibility, and bioavailability. Understanding these changes can aid in developing new millet-based food products that meet consumer demands, enhance market potential, and support health claims. Overall, this area of research is still under-explored, and future research on emerging technologies and value-added millet products should take into account the relationship between composition and nutrient bioavailability.

小米是一种小粒、适应气候变化的谷物,在促进可持续粮食和营养安全方面具有重要意义。除了它们的农艺优势,小米营养丰富,含有广泛的植物化学物质,对人体健康有益。最近,这些传统谷物的消费量激增,全球的食品技术人员正在探索几种热传递加工方法,以开发出这些无麸质谷物的改良产品。因此,这项工作的目的是作为一种资源,涵盖最先进的已知信息,关于小米采用的传统和新兴的热加工技术。然而,确定一种特定的传统或新兴技术来精确改善营养或整体质量仍然是一个遥远的目标,因为这些技术的效果取决于诸如剂量、持续时间和其他与工艺相关的变量等因素,以及食品基质的动态。本文综述了常用的小米热加工方法及其对小米营养品质、消化率、生物可及性和生物利用度的影响。了解这些变化有助于开发新的以小米为基础的食品,以满足消费者的需求,提高市场潜力,并支持健康声明。总体而言,这一领域的研究仍处于探索阶段,未来新兴技术和增值小米产品的研究应考虑到成分与营养物质生物利用度之间的关系。
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引用次数: 0
Unveiling the chemical composition and biological bioactivity of two Amazonian Oenocarpus species 揭示两种亚马逊河栎属植物的化学成分和生物活性
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-16 DOI: 10.1007/s00217-025-04901-z
Stephanie Dias Soares, Adriana de Souza Lima, Cynthia Tereza Correa da Silva Miranda, Geovana Manzan Sales, Ana Lúcia Tasca Gois Ruiz, Robson da Silva Pontes, Jaime Paiva Lopes Aguiar, Henrique Silvano Arruda, Rodrigo Ramos Catharino, Iramaia Angélica Neri Numa, Gláucia Maria Pastore

This study evaluated the nutritional and chemical profiles and the biological potential of Amazonian berries, including one fruit studied comprehensively for the first time. Oenocarpus bacaba (OB) and Oenocarpus minor (OM) showed considerable levels of proteins, lipids, insoluble fibers, and potassium. The phenolic profile mainly consisted of catechin and apigenin in both, with total phenolic content ranging from 1317.78 to 1387.22 mg GAE/100 g and anthocyanins between 1.04 and 5.59 mg C3G/100 g, in OB and OM, respectively. Antioxidant assays indicated high FRAP, followed by ORAC, mainly for OB. In antiproliferative activity evaluation, OB fluid extract was inactive (GI50 > 150 µg/mL) against a panel of tumor and non-tumor human cell lines. In the scratch assay, OB fluid extract significantly inhibited keratinocyte migration at 100 µg/mL. These findings support the functional food potential of OB and OM, particularly for antioxidant and wound-healing applications.

Graphical abstract

本研究评估了亚马逊浆果的营养、化学特征和生物学潜力,其中包括首次全面研究的一种水果。bacaba (OB)和Oenocarpus minor (OM)的蛋白质、脂质、不溶性纤维和钾含量相当高。黄芪和黄芪的酚类成分主要为儿茶素和芹菜素,总酚含量分别为1317.78 ~ 1387.22 mg GAE/100 g,花青素含量分别为1.04 ~ 5.59 mg C3G/100 g。抗氧化实验表明,OB液提取物具有较高的FRAP,其次是ORAC,主要针对OB。在抗增殖活性评估中,OB液提取物对一组肿瘤和非肿瘤人细胞系无活性(GI50 > 150µg/mL)。在划痕实验中,100µg/mL的OB液提取物显著抑制角质形成细胞的迁移。这些发现支持了OB和OM的功能性食品潜力,特别是在抗氧化和伤口愈合方面的应用。图形抽象
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引用次数: 0
Unlocking the potential of chia seeds: from nutritional powerhouse to food innovation 释放奇亚籽的潜力:从营养发电站到食品创新
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-16 DOI: 10.1007/s00217-025-04919-3
Faiyaz Ahmed, Muhammad Asif, Sammra Maqsood, Sadaf Shakoor, Ayesha Saddiqa

Chia seeds are a valuable component of functional foods due to their rich content of bioactive compounds such as phenolics, omega-3 fatty acids, proteins, dietary fiber, and essential minerals. Their rich phytochemical profile offers several health benefits, including antidiabetic, cardioprotective, anti-inflammatory, and antioxidant effects. This review offers an in-depth review of the phytochemical composition of chia seeds, their medicinal applications, and their roles in food science and technology. Particular emphasis is placed on their use in natural food preservatives, functional foods, and the development of applications such as bioplastics and edible films. Despite their potential, research is required to address challenges associated with sustainability, consistency, and bioavailability. The review also outlines future research priorities for sustainable food development and optimization of chia-derived phytochemicals optimization.

Graphical abstract

奇亚籽是一种有价值的功能性食品,因为它们含有丰富的生物活性化合物,如酚类物质、omega-3脂肪酸、蛋白质、膳食纤维和必需矿物质。它们丰富的植物化学成分提供了几种健康益处,包括抗糖尿病、心脏保护、抗炎和抗氧化作用。本文就奇亚籽的植物化学成分、药用价值及其在食品科学技术中的作用进行了综述。特别强调的是它们在天然食品防腐剂、功能性食品中的应用,以及生物塑料和可食用薄膜等应用的开发。尽管它们具有潜力,但仍需要研究解决与可持续性、一致性和生物利用度相关的挑战。综述还概述了可持续食品开发和中国植物化学物质优化的未来研究重点。图形抽象
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引用次数: 0
Advances in the use of Zein in food formulations: a comprehensive review with bibliometric analysis 玉米蛋白在食品配方中的应用进展:文献计量分析的综合综述
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-10 DOI: 10.1007/s00217-025-04922-8
Larissa Resende Matheus, Luiz Eduardo Nochi Castro, Vanessa Cosme Ferreira, Tânia Forster-Carneiro, Leda Maria Saragiotto Colpini

Zein, a prolamin protein derived from corn, has attracted increasing attention as a versatile and sustainable biomaterial for applications in the food industry. This review provides a comprehensive analysis of the origin of zein, extraction and purification methodologies, and eco-friendly approaches to extraction and modification, such as ultrasound- and enzyme-assisted techniques. Advances in physical, chemical, and biological modification strategies have improved the functional properties of zein, enabling its use in smart packaging, nanoencapsulation systems, and controlled-release platforms. Zein-based materials have shown significant potential in exhibiting antioxidant, antimicrobial, and barrier properties, particularly when combined with natural compounds or other biopolymers. The wide range of potential applications highlights the versatility of zein and reinforces its relevance to environmental sustainability. Nevertheless, challenges remain regarding scalability, regulatory approval, and the standardized evaluation of biodegradability and long-term food safety. Future research should prioritize green extraction protocols, the valorization of agricultural residues within biorefinery frameworks, and techno-economic assessments to support commercial viability. This review identifies current limitations and knowledge gaps while highlighting future directions that could drive the development of high-performance zein-based materials for food systems.

Graphical abstract

玉米蛋白是一种从玉米中提取的蛋白,作为一种多用途和可持续的生物材料在食品工业中的应用越来越受到人们的关注。本文综述了玉米蛋白的来源,提取和纯化方法,以及超声波和酶辅助技术等生态友好的提取和修饰方法。物理、化学和生物修饰策略的进步改善了玉米蛋白的功能特性,使其能够用于智能包装、纳米封装系统和控释平台。玉米蛋白基材料在抗氧化、抗菌和屏障性能方面显示出巨大的潜力,特别是当与天然化合物或其他生物聚合物结合时。广泛的潜在应用突出了玉米蛋白的多功能性,并加强了其与环境可持续性的相关性。然而,在可扩展性、监管批准以及生物降解性和长期食品安全的标准化评估方面,挑战仍然存在。未来的研究应优先考虑绿色提取方案、生物炼制框架内农业残留物的价值评估以及支持商业可行性的技术经济评估。这篇综述指出了目前的局限性和知识差距,同时强调了未来的方向,可以推动高性能玉米蛋白基材料的发展,用于食品系统。图形抽象
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引用次数: 0
A review of various protein separation techniques and valorization of cheese whey and buttermilk 评述了各种蛋白质分离技术及其对奶酪乳清和酪乳的增值作用
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-09 DOI: 10.1007/s00217-025-04877-w
Hadid Sukmana, Izza Rizwana, Réka Dobozi, Aws N. Al-Tayawi, Balázs P. Szabó, Barbaros Özer, Szabolcs Kertész

The dairy industry generates large quantities of nutrient-rich by-products, particularly cheese whey, and buttermilk, which are valuable sources of bioactive proteins and phospholipids. Whey proteins exhibit notable therapeutic potential, offering various health benefits such as antihypertensive effects, immune modulation, and opioid antagonism. Similarly, buttermilk-derived phospholipids demonstrate bioactive properties, including cholesterol reduction, anti-inflammatory activity, and neuroprotective effects. This review critically assesses recent advances in protein separation techniques, comparing conventional thermal methods, known to potentially compromise protein functionality, with emerging non-thermal approaches such as high-pressure processing and membrane filtration, which enable efficient recovery while preserving bioactivity. Additionally, advanced methodologies such as multi-level processing and enzymatic hydrolysis further expand the protein separation techniques. Key technical challenges, including high mineral content in whey and the low solids concentration in buttermilk, are discussed alongside potential innovative solutions. Whey’s elevated mineral levels can reduce protein isolation efficiency and diminish the emulsifying properties of whey proteins, potentially restricting their functional applications. Meanwhile, the low total solids content in buttermilk poses challenges in achieving high protein concentration yields, requiring advanced separation techniques. Further, this review examines contemporary trends in cheese whey and buttermilk valorization, highlighting their opportunities for enhanced food applications. It also explores cross-sectoral utilization in biofuels, biodegradable materials, pharmaceuticals, and agriculture, supporting economic and sustainability goals in the dairy sector.

乳制品工业产生大量营养丰富的副产品,特别是奶酪乳清和酪乳,它们是生物活性蛋白质和磷脂的宝贵来源。乳清蛋白表现出显著的治疗潜力,提供各种健康益处,如抗高血压作用,免疫调节和阿片类拮抗剂。同样,酪乳衍生的磷脂显示出生物活性特性,包括降低胆固醇、抗炎活性和神经保护作用。这篇综述批判性地评估了蛋白质分离技术的最新进展,将传统的热方法(已知可能损害蛋白质功能)与新兴的非热方法(如高压处理和膜过滤)进行了比较,这些方法在保持生物活性的同时能够有效地回收。此外,多层次加工和酶解等先进方法进一步扩展了蛋白质分离技术。关键的技术挑战,包括高矿物质含量的乳清和低固体浓度的酪乳,讨论了潜在的创新解决方案。乳清中矿物质含量的升高会降低蛋白质的分离效率,降低乳清蛋白的乳化特性,潜在地限制了它们的功能应用。同时,酪乳中总固体含量低,对实现高蛋白质浓度产率提出了挑战,需要先进的分离技术。此外,本综述探讨了奶酪乳清和酪乳增值的当代趋势,强调了它们在增强食品应用方面的机会。它还探讨了生物燃料、可生物降解材料、药品和农业的跨部门利用,支持乳制品行业的经济和可持续发展目标。
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引用次数: 0
Exopolysaccharides produced by lactic acid bacteria from kefir: production and optimization of cultivation conditions 开菲尔乳酸菌产胞外多糖的生产及培养条件优化
IF 3.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-10-06 DOI: 10.1007/s00217-025-04920-w
Caroline Krause Bierhals, Maria Fernanda Fernandes Siqueira, Silvana de Souza Sigali, Isabela Schneid Kroning, Igor Henrique de Lima Costa, Khadija Bezerra Massaut, Graciela Volz Lopes, Wladimir Padilha da Silva, Ângela Maria Fiorentini

The aim of this study was to isolate and characterize lactic acid bacteria (LAB) producing exopolysaccharides (EPS) from kefir and to optimize the cultivation conditions using a Rotatable Central Composite Design (RCCD) to increase biopolymer production. From kefir grains (five samples) and the fermented milk obtained, 44 LAB isolates were selected, of which eight were considered microbiologically safe and exhibited desirable technological characteristics. Isolate KLM6, molecularly identified as Leuconostoc mesenteroides, was selected for cultivation condition optimization, resulting in a 105.1% increase in EPS yield compared to previous results, using standard fermentation parameters (pH 6.2 and incubation at 37 °C for 24 h). This increase was due to the interplay of pH and temperature variables (pH 5.0 and incubation at 50.5 °C for 24 h), achieving a maximum EPS concentration of 0.841 g/L. It was concluded that alterations in cultivation conditions were determinative for the synthesis of this biopolymer, enabling the efficient future application of L. mesenteroides KLM6 in the food industry, since EPS-producing LAB are valued for improving the texture and viscosity of fermented products.

本研究旨在分离和鉴定产胞外多糖(EPS)的乳酸菌(LAB),并采用可旋转中心复合设计(RCCD)优化培养条件,以提高生物聚合物的产量。从所获得的开菲尔颗粒(5个样品)和发酵乳中筛选出44株乳酸菌,其中8株被认为微生物安全且具有理想的工艺特性。选择分子鉴定为Leuconostoc mesenteroides的分离株KLM6进行培养条件优化,在标准发酵参数(pH 6.2, 37℃孵育24 h)下,EPS产量比之前的结果提高了105.1%。这种增加是由于pH和温度变量(pH 5.0和50.5°C孵育24小时)的相互作用,EPS的最大浓度为0.841 g/L。综上所述,培养条件的改变对这种生物聚合物的合成起决定性作用,这使得产eps的乳酸菌KLM6在未来的食品工业中得到了有效的应用,因为它可以改善发酵产品的质地和粘度。
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引用次数: 0
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European Food Research and Technology
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