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Fisheye freshness detection using common deep learning algorithms and machine learning methods with a developed mobile application 使用常见的深度学习算法和机器学习方法,利用开发的移动应用程序进行鱼眼保鲜检测
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-18 DOI: 10.1007/s00217-024-04493-0
Muslume Beyza Yildiz, Elham Tahsin Yasin, Murat Koklu

Fish is commonly ingested as a source of protein and essential nutrients for humans. To fully benefit from the proteins and substances in fish it is crucial to ensure its freshness. If fish is stored for an extended period, its freshness deteriorates. Determining the freshness of fish can be done by examining its eyes, smell, skin, and gills. In this study, artificial intelligence techniques are employed to assess fish freshness. The author’s objective is to evaluate the freshness of fish by analyzing its eye characteristics. To achieve this, we have developed a combination of deep and machine learning models that accurately classify the freshness of fish. Furthermore, an application that utilizes both deep learning and machine learning, to instantly detect the freshness of any given fish sample was created. Two deep learning algorithms (SqueezeNet, and VGG19) were implemented to extract features from image data. Additionally, five machine learning models to classify the freshness levels of fish samples were applied. Machine learning models include (k-NN, RF, SVM, LR, and ANN). Based on the results, it can be inferred that employing the VGG19 model for feature selection in conjunction with an Artificial Neural Network (ANN) for classification yields the most favorable success rate of 77.3% for the FFE dataset.

鱼通常作为人类蛋白质和必需营养素的来源而被摄入。要充分享受鱼肉中的蛋白质和物质,确保鱼肉新鲜至关重要。如果鱼存放时间过长,其新鲜度就会下降。判断鱼的新鲜度可以通过检查鱼的眼睛、气味、鱼皮和鱼鳃来实现。本研究采用人工智能技术来评估鱼的新鲜度。作者的目标是通过分析鱼的眼睛特征来评估鱼的新鲜度。为此,我们开发了一种深度学习和机器学习相结合的模型,可以准确地对鱼的新鲜度进行分类。此外,我们还创建了一个同时利用深度学习和机器学习的应用程序,以即时检测任何给定鱼类样本的新鲜度。我们采用了两种深度学习算法(SqueezeNet 和 VGG19)从图像数据中提取特征。此外,还应用了五种机器学习模型来对鱼类样本的新鲜程度进行分类。机器学习模型包括(k-NN、RF、SVM、LR 和 ANN)。根据结果可以推断,采用 VGG19 模型进行特征选择,并结合人工神经网络(ANN)进行分类,对 FFE 数据集的成功率最高,达到 77.3%。
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引用次数: 0
Exploring the effect of sucrose and d-allulose addition on the gelling ability and physical properties of agar–agar vegan gels 探索蔗糖和 d-阿洛糖添加量对琼脂-琼脂素凝胶胶凝能力和物理性质的影响
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-16 DOI: 10.1007/s00217-024-04534-8
Pelin Poçan, Sevil Çıkrıkcı Erünsal

Plant-based hydrocolloid systems which are mixed with different carbohydrate types are becoming popular due to vegan concerns for both non-food and food industry. In this study, different sugar types (d-allulose and sucrose) at different concentrations (20% and 40%) were used to explore their effects on the gelation and physical properties of agar–agar. For characterization of these agar–agar-based gel systems, in addition to common physical methods (moisture content, water activity, color, hardness, viscosity), novel methods such as TD-NMR relaxometry, and FTIR were also used to get deep insight of the microstructures and water dynamics of the gel systems. Results clearly indicated that sugar type and concentration were vital factors affecting the agar–agar gel properties and dynamics of the system. Utilization of allulose in the gel systems led to formation of less moist and softer agar–agar matrix characterized with longer T2 spin–spin relaxation times. This study will lead to designing new types of vegan agar–agar-based confectionery products such as soft candies that are considered as perfect composite gels.

由于非食品和食品行业对素食的关注,混合了不同碳水化合物类型的植物基水胶体系统正变得越来越受欢迎。本研究使用了不同浓度(20% 和 40%)的不同糖类(d-阿洛糖和蔗糖)来探讨它们对琼脂-琼脂凝胶化和物理性质的影响。在表征这些琼脂-琼脂凝胶体系时,除了使用常见的物理方法(含水量、水活性、颜色、硬度、粘度)外,还使用了 TD-NMR 驰豫测定法和傅立叶变换红外光谱法等新方法,以深入了解凝胶体系的微观结构和水动力学。结果清楚地表明,糖的种类和浓度是影响琼脂-琼脂凝胶特性和系统动态的重要因素。在凝胶体系中使用阿尿糖会使琼脂-琼脂基质更湿润、更柔软,并具有更长的 T2 自旋-自旋弛豫时间。这项研究将有助于设计新型素食琼脂-琼脂基糖果产品,如被视为完美复合凝胶的软糖。
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引用次数: 0
Enhancing olive oil quality through an advanced enrichment process utilizing ripe and fallen fruits 通过利用成熟落果的先进浓缩工艺提高橄榄油质量
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-16 DOI: 10.1007/s00217-024-04515-x
Mariem Habibi, Marwa Abdallah, Wael Taamali, Alessandro Genovese, Andrea Balivo, Jose Manuel Lorenzo, Najla Trabelsi

Enriched olive oil combines olive oil with plants for enhanced flavor and potential health benefits, offering a versatile culinary ingredient. This process combines the natural goodness of olive oil with the unique characteristics of plants. The aim of this work was to study the effect of rosemary (Rosmarinus officinalis) as a food additive (E 392) in the enrichment of ripe and fallen olives on the quality of the resulting flavored olive oil. Olive fruits at different stages of maturation green (ST1), mixed (ST2), black (ST3), and fallen in the ground (FO) were crushed with rosemary at two different percentages (2 and 5% w/w). Quality parameters, fatty acid profile, antioxidant profile, and volatile composition were evaluated. For ST3 and FO, rosemary addition decreased free acidity values, peroxide index, K232, and pigment content. Total phenols increased by 34 and 55% for ST3 and FO, respectively with 2% rosemary. As for bio-phenols, this process influenced antioxidant activity. In fact, 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity is improved by 59 and 51%, respectively, for ST3 and FO oils flavored with 5% rosemary. As anticipated, the volatile profile changed significantly due to the rosemary addition, inducing the presence of terpene compounds, such as α-pinene, α-cymene, camphene, β-pinene, and α-terpineol. These findings prove that aromatization process improves the quality and the antioxidant profile of olive oils obtained from mature and fallen olives, and could help in solving the economic problems of oil mills. Other plants such as garlic, lemon, thyme, and others can be studied to find out if better results can be achieved.

浓缩橄榄油将橄榄油与植物相结合,使其风味更佳,并具有潜在的保健功效,是一种多功能的烹饪配料。这一工艺将橄榄油的天然美味与植物的独特特性相结合。这项工作的目的是研究迷迭香(Rosmarinus officinalis)作为一种食品添加剂(E 392),在富集成熟橄榄果和落叶橄榄果的过程中对所得风味橄榄油质量的影响。将处于不同成熟阶段的绿色(ST1)、混合(ST2)、黑色(ST3)和落地(FO)橄榄果与迷迭香以两种不同的比例(2%和 5%w/w)混合压碎。对质量参数、脂肪酸含量、抗氧化剂含量和挥发性成分进行了评估。对于 ST3 和 FO,迷迭香的添加降低了游离酸值、过氧化物指数、K232 和色素含量。在添加 2% 迷迭香的 ST3 和 FO 中,总酚分别增加了 34% 和 55%。至于生物酚,这一过程影响了抗氧化活性。事实上,添加了 5%迷迭香的 ST3 和 FO 油的 2,2-二苯基-1-苦基肼(DPPH)活性分别提高了 59% 和 51%。正如预期的那样,由于添加了迷迭香,挥发性特征发生了显著变化,诱发了萜烯化合物的出现,如 α-蒎烯、α-栀子烯、莰烯、β-蒎烯和α-松油醇。这些研究结果证明,芳香化过程可以提高从成熟橄榄果和落果中提取的橄榄油的质量和抗氧化性,有助于解决油厂的经济问题。还可以对大蒜、柠檬、百里香等其他植物进行研究,看看能否取得更好的效果。
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引用次数: 0
Chemical, microbial, and volatile compounds of water kefir beverages made from chickpea, almond, and rice extracts 用鹰嘴豆、杏仁和大米提取物制成的开菲尔水饮料中的化学、微生物和挥发性化合物
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-16 DOI: 10.1007/s00217-024-04533-9
Mutlu Ustaoğlu-Gençgönül, Çağlar Gökırmaklı, Bilgenur Üçgül, Yonca Karagül-Yüceer, Zeynep B. Guzel-Seydim

This study aims to assess the microbial, nutritional, volatile, and sensory characteristics of rice, almond, and chickpea water kefir beverages during refrigerated storage. Plant-based kefirs contained significant amounts of lactic acid bacteria and yeasts. The microbial content of kefirs was stable during 14-day refrigerated storage. Lactic acid, acetic acid, and tartaric acid are commonly detected organic acids in kefir samples. Almond and chickpea kefirs were rich in potassium mineral. Almond kefir had the highest ethanol content among plant-based kefirs, followed by chickpea and rice-based kefirs. Ethyl acetate, acetic acid, propionic acid, hexanoic acid, and benzenemethanol were identified as key volatile compounds in almond kefir and chickpea kefir samples using a GC–MS detector during water kefir fermentation. According to sensory analysis results, significant differences are present for all test parameters except odor. Almond kefir was the most accepted, while the other two kefir samples were below the general acceptance level (P < 0.05).

本研究旨在评估大米、杏仁和鹰嘴豆水克菲尔饮料在冷藏储存期间的微生物、营养、挥发性和感官特性。植物基酸乳酒含有大量乳酸菌和酵母。在 14 天的冷藏储存期间,酸乳酒中的微生物含量保持稳定。乳酸、乙酸和酒石酸是在酸乳酒样品中普遍检测到的有机酸。杏仁和鹰嘴豆酸乳含有丰富的钾矿物质。在以植物为原料的酸乳中,杏仁酸乳的乙醇含量最高,其次是鹰嘴豆和大米酸乳。利用气相色谱-质谱检测器确定了杏仁酸乳酒和鹰嘴豆酸乳酒样品中的乙酸乙酯、乙酸、丙酸、己酸和苯甲醇是水酸乳酒发酵过程中的主要挥发性化合物。根据感官分析结果,除气味外,所有测试参数都存在显著差异。杏仁酸乳的接受度最高,而其他两种酸乳样品的接受度低于一般接受水平(P < 0.05)。
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引用次数: 0
Determination of phenolic content and bioactive characterization of Anatolian propolis 测定安纳托利亚蜂胶的酚含量和生物活性特征
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-15 DOI: 10.1007/s00217-024-04522-y
Zehra Can, Ceren Birinci, Yakup Kara, Ülkü Zeynep Üreyen Esertaş, Sevgi Kolaylı

In this study, propolis referred to as of Türkiye Anatolian propolis was utilized. This work was aim to prepare a 70% ethanolic extract of propolis and subsequently determine certain biochemical parameters, total phenolic content (TPC), total flavonoid content (TFC), phenolic and flavonoid composition, inhibitory effects on certain enzymes [acetylcholinesterase (AChE), urease, and alpha-amylase], along with assessments of antiviral activity against Vero cells, lung cancer cells (A549), and breast cancer cells (MDA-MB-231). The richness of phenolic content in Anatolian propolis clearly indicates a high inhibitory effect on these enzymes. Additionally, it was observed to exert significant effects on cancer cells and demonstrated effective antiviral activity. It can be contemplated that the Anatolian propolis might serve as a potential source for novel drugs, and the isolation of its compounds could offer opportunities for utilization in traditional medicine.

在这项研究中,使用的是土耳其安纳托利亚蜂胶。这项工作旨在制备 70% 的蜂胶乙醇提取物,然后测定某些生化参数、总酚含量(TPC)、总黄酮含量(TFC)、酚类和黄酮类成分、对某些酶(乙酰胆碱酯酶(AChE)、脲酶和α-淀粉酶)的抑制作用,以及对 Vero 细胞、肺癌细胞(A549)和乳腺癌细胞(MDA-MB-231)的抗病毒活性评估。安纳托利亚蜂胶中丰富的酚类成分清楚地表明它对这些酶具有很强的抑制作用。此外,还观察到蜂胶对癌细胞有显著效果,并显示出有效的抗病毒活性。可以认为,安纳托利亚蜂胶可作为新型药物的潜在来源,其化合物的分离可为传统医药的利用提供机会。
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引用次数: 0
Pitahaya (Hylocereus ocamponis) peel flour as new ingredient in the development of beef burgers: impact on the quality parameters 皮塔哈雅(Hylocereus ocamponis)果皮粉作为开发牛肉汉堡的新配料:对质量参数的影响
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-15 DOI: 10.1007/s00217-024-04545-5
Verónica Reyes-García, Carmen Botella-Martínez, Naida Juárez-Trujillo, Manuel Viuda-Martos

The aim of this work was to determine the impact of pitahaya peel flour (PPF) addition, at different concentrations (1.5 and 3%) on the chemical, physico-chemical and sensorial properties of beef burgers. This assessment was based on: proximal analysis, physico-chemical and cooking properties, lipid oxidation and sensory assay of beef burgers. The burger reformulation with pitahaya peel flour had no effect (p > 0.05) on the chemical composition of raw burgers, whilst the cooked burger increased (p < 0.05) the protein and fat content and in the same way increased the calcium, potassium and phosphorus content (p < 0.05) with respect to the control sample. The colour coordinates redness (increased) and yellowness (decreased) were affected (p < 0.05) by the pitahaya peel flour addition. From a textural point of view, all parameters, except springiness, were affected (p < 0.05) by the incorporation of pitahaya peel flour. In addition, the use of pitahaya peel flour reduces (p < 0.05) the cooking loss and the shrinkage of burgers. The raw and cooked beef burger added with pitahaya peel flour showed lower lipid oxidation values (p < 0.05) than those of the control sample. The hedonic scores for all burgers analysed ranged from 6 to 7, which indicates a high degree of acceptance by the panellists. The results obtained indicated that pitahaya peel flour could be used as a novel ingredient in the development of beef burgers.

这项研究旨在确定添加不同浓度(1.5% 和 3%)的番荔枝皮粉(PPF)对牛肉汉堡的化学、理化和感官特性的影响。评估基于牛肉汉堡的近端分析、理化和烹饪特性、脂质氧化和感官检测。与对照样品相比,用番木瓜皮粉重新配制的汉堡对生汉堡的化学成分没有影响(p >0.05),而煮熟的汉堡增加了蛋白质和脂肪含量(p <0.05),同样也增加了钙、钾和磷含量(p <0.05)。颜色坐标红度(增加)和黄度(减少)受添加的番木瓜皮粉的影响(p <0.05)。从质地的角度来看,除回弹性外,所有参数都会受到加入番木瓜皮粉的影响(p < 0.05)。此外,番木瓜皮粉还能减少汉堡的烹饪损失和收缩(p < 0.05)。与对照样品相比,添加了番木瓜皮粉的生牛肉汉堡和熟牛肉汉堡的脂质氧化值更低(p < 0.05)。所分析的所有汉堡的享乐性得分都在 6 到 7 分之间,这表明小组成员的接受程度很高。结果表明,番木瓜皮粉可作为一种新型配料用于牛肉汉堡的开发。
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引用次数: 0
Performance of vision transformer and swin transformer models for lemon quality classification in fruit juice factories 用于果汁厂柠檬质量分类的视觉变换器和斯温变换器模型的性能
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-15 DOI: 10.1007/s00217-024-04537-5
Sezer Dümen, Esra Kavalcı Yılmaz, Kemal Adem, Erdinç Avaroglu

Assessing the quality of agricultural products holds vital significance in enhancing production efficiency and market viability. The adoption of artificial intelligence (AI) has notably surged for this purpose, employing deep learning and machine learning techniques to process and classify agricultural product images, adhering to defined standards. This study focuses on the lemon dataset, encompassing ‘good’ and ‘bad’ quality classes, initiate by augmenting data through rescaling, random zoom, flip, and rotation methods. Subsequently, employing eight diverse deep learning approaches and two transformer methods for classification, the study culminated in the ViT method achieving an unprecedented 99.84% accuracy, 99.95% recall, and 99.66% precision, marking the highest accuracy documented. These findings strongly advocate for the efficacy of the ViT method in successfully classifying lemon quality, spotlighting its potential impact on agricultural quality assessment.

评估农产品质量对提高生产效率和市场生存能力至关重要。为此,人工智能(AI)的应用显著增加,它采用深度学习和机器学习技术,按照规定的标准对农产品图像进行处理和分类。本研究的重点是柠檬数据集,包括 "好 "和 "坏 "两个质量等级,通过重新缩放、随机缩放、翻转和旋转等方法增强数据。随后,研究采用了八种不同的深度学习方法和两种转换器方法进行分类,最终 ViT 方法获得了前所未有的 99.84% 的准确率、99.95% 的召回率和 99.66% 的精确率,创下了有记录以来的最高准确率。这些发现有力地证明了 ViT 方法在成功对柠檬质量进行分类方面的功效,凸显了其对农业质量评估的潜在影响。
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引用次数: 0
Vegan and sugar-substituted chocolates: assessing fatty acids, methylxanthines, minerals, volatiles and sensory profiles 素食巧克力和代糖巧克力:评估脂肪酸、甲基黄嘌呤、矿物质、挥发性物质和感官特征
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-15 DOI: 10.1007/s00217-024-04547-3
M. Pollon, F. Torregrossa, E. Marconi, C. Cinquanta, C. Messia, F. Malvano, F. Mazza, D. Planeta, O. Corona

To satisfy the ever-increasing share of consumers who are lactose intolerant, vegan or require foods with less simple sugars, chocolate companies are constantly looking for new formulations. In the present research, the nutritional and sensory profile of two novel chocolates was evaluated. In the first, milk powder was replaced by coconut copra, almond and isolated soy protein, and in the second dark chocolate-based, sucrose was replaced by coconut sugars, stevia and erythritol, resulting in a reduction in calorific value of about 8%. The substitution of milk powder with vegetal ingredients affected the chocolate mineral profile, with high iron and copper concentrations and less potassium, calcium and phosphorus. The vegan samples showed the presence of medium-chain fatty acids from coconut oil, mainly lauric acid. Esters, the group responsible for the fruity aroma, represented mainly by benzaldehyde, furfural and vanillin, were prevalent in the vegan sample. The novel chocolate without added sucrose provides approximately 2182.6 kJ/hg registering a reduction in caloric value of more than 8% compared to the standard sample. In addition, the presence of coconut sugar, with its low glycaemic index (35), made the novel chocolate healthier than plain sucrose-containing dark chocolate.

为了满足越来越多的乳糖不耐受者、素食者或需要少单糖食品的消费者的需求,巧克力公司一直在寻找新的配方。本研究对两种新型巧克力的营养和感官特性进行了评估。在第一种巧克力中,奶粉被椰干、杏仁和分离大豆蛋白取代;在第二种黑巧克力中,蔗糖被椰子糖、甜叶菊和赤藓糖醇取代,热量值降低了约 8%。用植物成分替代奶粉影响了巧克力的矿物质含量,铁和铜的含量较高,而钾、钙和磷的含量较低。纯素样品中含有椰子油中的中链脂肪酸,主要是月桂酸。酯类物质是水果香味的主要成分,在纯素样品中普遍存在,主要表现为苯甲醛、糠醛和香兰素。不添加蔗糖的新型巧克力可提供约 2182.6 千焦/小时克的热量,与标准样品相比,热量降低了 8%以上。此外,椰子糖的低血糖指数(35)使新型巧克力比普通含蔗糖黑巧克力更健康。
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引用次数: 0
Characterizing the non-starch polysaccharides of hempseed cell walls 大麻籽细胞壁非淀粉多糖的特征
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-15 DOI: 10.1007/s00217-024-04548-2
Miranda R. Agbana, Brynn S. Angeletti, Hanna C. Buecker, Yen-Chang Tseng, Brittany E. Davis, Rachel R. Schendel

Hempseed (Cannabis sativa L.) is a valuable source of oil and a quality vegetable protein source, but little is known about its fiber composition. This study provides the most extensive characterization of hempseed cell wall polysaccharides to date. Quantification of total dietary fiber (TDF) and acetyl bromide soluble lignin (ABSL), monosaccharide composition through two complimentary hydrolysis methods (Saeman hydrolysis and methanolysis), and linkage analysis via derivatization of monosaccharides to partially methylated alditol acetates (PMAAs) were done. More detailed profiling of hempseed xyloglucans and pectic arabinans and galactans was performed via targeted enzymatic generation and quantification of characteristic oligosaccharides. Hempseed’s cell wall polysaccharide profile is consistent with the primary cell walls of dicots, but it is particularly abundant in linear xylans, presumably from secondary cell walls. The hempseed water-insoluble cell wall material was mostly composed of cellulose and xylans, but pectins (including arabinans with a low degree of branching and galactans), xyloglucans with L- and F-motifs and a relatively low substitution rate, and mannans were also present. These data show that hempseed is a good source of complex dietary fiber for human diets and a potential fiber additive for herbivorous livestock diets. Potential consequences of these carbohydrate structural details on hempseed’s microbial fermentability are discussed.

大麻籽(Cannabis sativa L.)是一种宝贵的油脂来源和优质植物蛋白来源,但人们对其纤维成分却知之甚少。这项研究提供了迄今为止最广泛的大麻籽细胞壁多糖特征。研究人员对总膳食纤维(TDF)和乙酰溴可溶性木质素(ABSL)进行了定量,通过两种互补的水解方法(塞曼水解法和甲醇分解法)对单糖成分进行了分析,并通过将单糖衍生为部分甲基化醛醇醋酸酯(PMAAs)进行了连接分析。通过有针对性的酶法生成和定量特征低聚糖,对大麻籽木糖、果胶阿拉伯聚糖和半乳聚糖进行了更详细的分析。大麻籽的细胞壁多糖特征与双子叶植物的初级细胞壁一致,但它的线性木聚糖特别丰富,可能来自次级细胞壁。大麻籽的水不溶性细胞壁物质主要由纤维素和木聚糖组成,但也存在果胶(包括分支程度较低的阿拉伯聚糖和半乳聚糖)、具有 L-和 F-位点且取代率相对较低的木聚糖以及甘露聚糖。这些数据表明,大麻籽是人类膳食中复杂膳食纤维的良好来源,也是草食牲畜膳食中潜在的纤维添加剂。本文讨论了这些碳水化合物结构细节对大麻籽微生物发酵性的潜在影响。
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引用次数: 0
Potential hypoglycemic activity of anthocyanidins monoglucoside and diglucoside from Yan 73 (Vitis vinifera L.) and spine grape (Vitis davidii Foex)—effect on α-glucosidases of gut microbiota and human Caco-2 cells Yan 73(葡萄属)和刺葡萄(Vitis davidii Foex)花青素单葡萄糖苷和双葡萄糖苷的潜在降血糖活性--对肠道微生物群和人 Caco-2 细胞中 α-葡萄糖苷酶的影响
IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-14 DOI: 10.1007/s00217-024-04524-w
Runyu Liu, Wenxiu Yue, Luye Yang, Yifan Wang, Jin Liu, Fuliang Han

Malvidin-3-O-glucoside (mv-3-O-glc), malvidin-3,5-O-diglucoside (mv-3,5-O-diglc), and malvidin-3-O-(6-O-coumaroyl) -glucoside-5-O-glucoside (mv-3,5-O-diglcCoum) extracted from Yan 73 (Vitis vinifera L.) and spine grape (Vitis davidii Foex) were used to investigate the effect on maltase and sucrase in the gut microbiota and Caco-2 cells. The results showed that three anthocyanins exhibited the inhibition of both maltase and sucrase in Caco-2 cells, and mv-3,5-O-diglc was the most effective one with an IC50 of 258.96 μM for maltase and 189 μM for sucrase, respectively, through a mixed-type inhibitory mechanism. The strong inhibition was also supported by the significant decrease in sucrase mRNA and protein levels in Caco-2 cells after treatment with mv-3,5-O-diglc for 4 h. Moreover, the inhibition effect of anthocyanins was observed only on maltase, which originated from the gut microbiota. The inhibition potential of mv-3-O-glc against maltase was higher than that of the other two anthocyanins in a mixed-type inhibition manner (IC50 = 9.66 ± 0.33 μM). Molecular docking revealed that mv-3-O-glc and mv-3,5-O-diglc formed hydrogen bonds with the aspartic acid (ASP) 443 catalytic residue of maltase, respectively, and then inhibited the enzyme activity by occupying the catalytic center. In particular, mv-3,5-O-diglc could easily fit into the active center of sucrase and form hydrogen bonds with the ASP1394 residue, which could be another reason for the inhibition of sucrase. Thus, anthocyanins, especially the diglucoside anthocyanins derived from grapes, are good potential α-glucosidase inhibitors for regulating postprandial blood glucose levels as dietary supplements.

研究人员利用从炎73(Vitis vinifera L.)和刺葡萄(Vitis davidii Foex)中提取的麦芽糖苷-3-O-葡萄糖苷(mv-3-O-glc)、麦芽糖苷-3,5-O-二葡萄糖苷(mv-3,5-O-diglc)和麦芽糖苷-3-O-(6-O-香豆酰)-葡萄糖苷-5-O-葡萄糖苷(mv-3,5-O-diglcoum)对肠道微生物群和 Caco-2 细胞中麦芽糖酶和蔗糖酶的影响。和刺葡萄(Vitis davidii Foex)中提取的花青素苷-5-O-葡萄糖苷(mv-3,5-O-diglcoum),研究其对肠道微生物群和 Caco-2 细胞中麦芽糖酶和蔗糖酶的影响。结果表明,3种花青素对Caco-2细胞中的麦芽糖酶和蔗糖酶均有抑制作用,其中mv-3,5-O-diglc的抑制作用最强,对麦芽糖酶的IC50为258.96 μM,对蔗糖酶的IC50为189 μM。此外,花青素只对麦芽糖酶有抑制作用,而麦芽糖酶来源于肠道微生物群。在混合型抑制作用下,mv-3-O-glc 对麦芽糖酶的抑制潜力高于其他两种花青素(IC50 = 9.66 ± 0.33 μM)。分子对接发现,mv-3-O-glc 和 mv-3,5-O-diglc 分别与麦芽糖酶的天冬氨酸(ASP)443 催化残基形成氢键,然后通过占据催化中心来抑制酶的活性。特别是,mv-3,5-O-diglc 很容易进入蔗糖酶的活性中心,并与 ASP1394 残基形成氢键,这可能是抑制蔗糖酶的另一个原因。因此,花青素,尤其是从葡萄中提取的二葡萄糖苷花青素,是一种很好的潜在α-葡萄糖苷酶抑制剂,可作为膳食补充剂调节餐后血糖水平。
{"title":"Potential hypoglycemic activity of anthocyanidins monoglucoside and diglucoside from Yan 73 (Vitis vinifera L.) and spine grape (Vitis davidii Foex)—effect on α-glucosidases of gut microbiota and human Caco-2 cells","authors":"Runyu Liu,&nbsp;Wenxiu Yue,&nbsp;Luye Yang,&nbsp;Yifan Wang,&nbsp;Jin Liu,&nbsp;Fuliang Han","doi":"10.1007/s00217-024-04524-w","DOIUrl":"10.1007/s00217-024-04524-w","url":null,"abstract":"<div><p>Malvidin-3-<i>O</i>-glucoside (mv-3-<i>O</i>-glc), malvidin-3,5-<i>O</i>-diglucoside (mv-3,5-<i>O</i>-diglc), and malvidin-3-<i>O</i>-(6-<i>O</i>-coumaroyl) -glucoside-5-<i>O</i>-glucoside (mv-3,5-<i>O</i>-diglcCoum) extracted from Yan 73 (<i>Vitis vinifera</i> L.) and spine grape (<i>Vitis davidii</i> Foex) were used to investigate the effect on maltase and sucrase in the gut microbiota and Caco-2 cells. The results showed that three anthocyanins exhibited the inhibition of both maltase and sucrase in Caco-2 cells, and mv-3,5-<i>O</i>-diglc was the most effective one with an IC<sub>50</sub> of 258.96 μM for maltase and 189 μM for sucrase, respectively, through a mixed-type inhibitory mechanism. The strong inhibition was also supported by the significant decrease in sucrase mRNA and protein levels in Caco-2 cells after treatment with mv-3,5-<i>O</i>-diglc for 4 h. Moreover, the inhibition effect of anthocyanins was observed only on maltase, which originated from the gut microbiota. The inhibition potential of mv-3-<i>O</i>-glc against maltase was higher than that of the other two anthocyanins in a mixed-type inhibition manner (IC<sub>50</sub> = 9.66 ± 0.33 μM). Molecular docking revealed that mv-3-<i>O</i>-glc and mv-3,5-<i>O</i>-diglc formed hydrogen bonds with the aspartic acid (ASP) 443 catalytic residue of maltase, respectively, and then inhibited the enzyme activity by occupying the catalytic center. In particular, mv-3,5-<i>O</i>-diglc could easily fit into the active center of sucrase and form hydrogen bonds with the ASP1394 residue, which could be another reason for the inhibition of sucrase. Thus, anthocyanins, especially the diglucoside anthocyanins derived from grapes, are good potential α-glucosidase inhibitors for regulating postprandial blood glucose levels as dietary supplements.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"250 8","pages":"2111 - 2122"},"PeriodicalIF":3.0,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140704511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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European Food Research and Technology
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