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Effect of different stand densities on xylem and phloem formation in Norway spruce plantations 不同林分密度对挪威云杉人工林木质部和韧皮部形成的影响
3区 农林科学 Q2 FORESTRY Pub Date : 2023-10-16 DOI: 10.1163/22941932-bja10140
Kyriaki Giagli, Hanuš Vavrčík, Dimitrios Tsalagkas, Jakub Černý, Jan Leugner, Jana Hacurová, Vladimír Gryc
Summary Preliminary results of an investigation of the thinning effect on Norway spruce tree growth in terms of xylogenesis and phloemogenesis are presented. Three plots were selected where the stand densities were reduced by pre-commercial thinning to 1800 trees/ha (plot A; mild thinning) and 1300 trees/ha (plot C; heavy thinning) in February 2020. Plot B had no silvicultural intervention and represented a control variant (4500 trees/ha). Three dominant 14-year-old Norway spruce trees were sampled (micro-cored) for studying the xylem and phloem formation in the 2020 growing season. The total differentiation duration was determined to be around 169.7 ± 12.7–179.3 ± 4.0 days. The average number of xylem cells formed in control plot B was only 140 mature cells, plots C and A were determined to have 175 and 200 mature cells, respectively. Plot A had the fastest growth rate of all the plots studied. In all three plots (A, B, C), the beginning of the early phloem, late phloem sieve cells, and axial parenchyma cells coincided. Nonetheless, in terms of total phloem cell increase, plot C displayed the fastest growth rate among the three plots studied. The first results showed that the rate of total increment in both xylem and phloem cells, as well as the total number of produced tracheids and sieve cells, seem to be positively affected after the thinning application.
本文从木质发生和韧皮形成两个方面对挪威云杉生长的间伐效应进行了初步研究。选择了三个样地,通过商业前间伐将林分密度降低到1800棵/公顷(样地A;轻度间伐)和1300棵/公顷(地块C;严重减薄)。B区未进行造林干预,为对照变异(4500棵/公顷)。为了研究2020年生长季节的木质部和韧皮部形成,对3棵优势14岁挪威云杉进行了取样(微芯)。总分化时间为169.7±12.7 ~ 179.3±4.0 d。对照区B平均形成的木质部细胞数仅为140个,C区和A区分别有175个和200个成熟细胞。A样地生长速度最快。在A、B、C三幅图中,早期韧皮部、晚期韧皮部筛子细胞和轴向薄壁细胞的开始时间重合。但是,就韧皮部细胞的总增长量而言,图C的生长速度是3个样地中最快的。结果表明:疏伐处理对木质部和韧皮部细胞的总生长率、管胞和筛细胞的总生长率均有显著的正向影响;
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引用次数: 1
Properties of Catalpa bungei (Bignoniaceae) earlywood and latewood in the same growth ring during moisture adsorption/desorption: swelling and shrinkage at the cell level 黄叶梓(biignoniaceae)早木和晚木在同一生长期吸湿/解吸过程中的特性:细胞水平上的肿胀和收缩
3区 农林科学 Q2 FORESTRY Pub Date : 2023-10-16 DOI: 10.1163/22941932-bja10138
Fangyu Yin, Jiali Jiang, Shixiong Sheng, Ping Wang, Yong Yang, Yafang Yin
Summary In this study, Dynamic Vapor Sorption Resolution combined with a Dino-Lite Edge Digital Microscope was used to document in real-time the swelling/shrinkage behavior of earlywood and latewood cells (wood fiber and vessel) on homogeneous samples of Catalpa bungei C.A.Mey. at the microscopic scale. The results showed that the lumen and cell wall of wood fibers swelled with the increase of relative humidity and shrank with the decrease of relative humidity in the sorption cycle of 0∼95% relative humidity condition. However, vessels showed an opposite trend to the behaviors mentioned above. In any region of both types of cells, latewood cell size (area and diameter) changed more significantly. For diameter change, the change of tangential diameter was larger than that of radial diameter. In addition, in the sorption cycle, the size (area and diameter) change of any region of wood fiber and vessel showed swelling hysteresis. Compared with earlywood cells, latewood cells had a more pronounced swelling hysteretic value, and the swelling hysteretic value in the tangential direction was higher than that in the radial direction in terms of diameter. Sorption hysteresis might be one of the contributing factors to swelling hysteresis. Finally, it was found that under any relative humidity, the dimensional changes of the wood fibers and the vessels were immediately stabilized when the moisture content reached its equilibrium.
在本研究中,采用动态蒸汽吸附分辨率结合Dino-Lite边缘数码显微镜,实时记录了紫杉树(Catalpa bungei C.A.Mey)均匀样品上早、晚木细胞(木纤维和血管)的膨胀/收缩行为。在微观尺度上。结果表明,在相对湿度为0 ~ 95%的条件下,木材纤维的管腔和细胞壁随相对湿度的增加而膨胀,随相对湿度的降低而收缩。然而,血管表现出与上述行为相反的趋势。在两种细胞的任何区域,红木细胞的大小(面积和直径)变化更显著。对于直径的变化,切向直径的变化大于径向直径的变化。此外,在吸附循环中,木纤维和容器的任何区域的尺寸(面积和直径)变化都表现出膨胀滞后。与早木细胞相比,晚木细胞肿胀滞回值更明显,且直径上切向肿胀滞回值大于径向肿胀滞回值。吸附滞后可能是造成溶胀滞后的原因之一。最后发现,在任何相对湿度下,当含水率达到平衡时,木材纤维和容器的尺寸变化立即稳定下来。
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引用次数: 0
Advanced imaging and quantification of the cambium and developing xylem in eucalypts using X-ray micro- and nano-computed tomography 利用X射线微纳计算机断层扫描技术对桉树形成层和发育中的木质部进行高级成像和定量
IF 1.9 3区 农林科学 Q2 FORESTRY Pub Date : 2023-08-18 DOI: 10.1163/22941932-bja10135
L. Schoeman, D. Drew
In recent years, the popularity of X-ray computed tomography (CT), as a non-destructive imaging technique, has continued to expand in various research domains. In wood research, X-ray CT has proven to be useful for three-dimensional (3D) structural studies investigating the complex tissues of trees. Wood formation (i.e., xylogenesis) initiates in the cambium and a narrow zone of subsequent differentiation, both of which play key roles in plant growth and development. However, the dynamics of xylogenesis in eucalypts remain relatively poorly understood, in large part due to challenges in sampling, imaging, and characterizing the cambium. Therefore, the aim of this study was to present a workflow to evaluate the feasibility of using X-ray CT to characterize and quantify the structural properties of the cambium in eucalypts. The growth responses of Corymbia hybrid seedlings, exposed to either irrigated or droughted conditions, was investigated by monitoring the structural development of the cambium. To track microstructural changes in the cambium, the same seedlings were imaged with X-ray micro-CT (μCT) one day before the treatments and again six days after the respective treatments. After the last X-ray μCT scan, X-ray nano-CT was also applied. Using image analysis techniques, the morphological characteristics of the cambium could be determined. X-ray μCT displayed a larger, thicker cambial zone in irrigated plants, while a much thinner cambium was visible in droughted seedlings. X-ray nano-CT revealed that droughted plants were associated with a significantly () smaller cambium volume containing smaller cells, compared to the cambium of irrigated plants. Light microscopy was used to validate the CT results and demonstrated no significant () difference in the cambium width and cell diameter obtained from the two respective CT techniques. The findings of this study proved X-ray CT to be a valuable tool for examining the effect of changing environmental conditions on the complex cambium structure of Corymbia hybrid seedlings.
近年来,X射线计算机断层扫描(CT)作为一种非破坏性成像技术,在各个研究领域不断扩大。在木材研究中,X射线CT已被证明可用于研究树木复杂组织的三维结构研究。木材形成(即木材发生)始于形成层和随后分化的狭窄区域,这两者在植物生长和发育中都起着关键作用。然而,对桉树木材形成的动力学仍知之甚少,这在很大程度上是由于形成层的采样、成像和表征方面的挑战。因此,本研究的目的是提出一个工作流程,以评估使用X射线CT来表征和量化桉树形成层结构特性的可行性。通过监测形成层的结构发育,研究了Corymbia杂交苗在灌溉或干旱条件下的生长反应。为了跟踪形成层的微观结构变化,在处理前一天和处理后六天用X射线显微CT(μCT)对相同的幼苗进行成像。在最后一次X射线μCT扫描后,还应用了X射线纳米CT。利用图像分析技术,可以确定形成层的形态特征。X射线μCT显示,灌溉植物的形成层更大、更厚,而干旱的幼苗的形成层则薄得多。X射线纳米CT显示,与灌溉植物的形成层相比,干旱植物的形成膜体积明显较小,含有较小的细胞。使用光学显微镜验证CT结果,并证明从两种不同的CT技术获得的形成层宽度和细胞直径没有显著差异。这项研究的结果证明,X射线CT是一种有价值的工具,可以用来研究环境条件的变化对伞菌杂交幼苗复杂形成层结构的影响。
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引用次数: 0
Study of antioxidant enzymes’ activity in reaction wood of poplar tree (Populus alba L.) 白杨反应材抗氧化酶活性的研究
IF 1.9 3区 农林科学 Q2 FORESTRY Pub Date : 2023-08-17 DOI: 10.1163/22941932-bja10136
Abasali Masoumi, K. Pourtahmasi, Reza Maali Amiri
Studying biochemical variation in reaction wood is needed to understand the nature of reaction wood formation and the physiology of trees. The activity of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX)) was studied in normal wood (NW) and reaction wood (RW) (opposite wood (OW) and tension wood (TW)) in the bole of poplar trees (Populus alba L.). Four-year-old trees were induced to produce reaction wood by forced bending. The activity of antioxidant enzymes was studied with repeated sampling during a growing season. The results showed that total protein content and antioxidant enzymes are sensitive to mechanically induced stress. TW and OW showed higher activity of enzymes than NW as well as higher amounts of total protein. OW showed a higher concentration of CAT and SOD than TW. APX was more active than GPX in both TW and OW. In addition to mechanical stress, the effect of other climate factors was observed in the reactive oxygen species accumulation.
研究反应材的生化变化是了解反应材形成的本质和树木生理的必要条件。研究了杨树(Populus alba L.)正常木材(NW)和反应木材(RW)(对生木(OW)和张紧木(TW))孔洞中抗氧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和愈创木酚过氧化物酶(GPX))的活性。通过强制弯曲,诱导4年树龄的树木产生反应木材。在一个生长季节反复取样,研究了抗氧化酶的活性。结果表明,总蛋白含量和抗氧化酶对机械胁迫敏感。TW和OW的酶活性高于NW,总蛋白含量也高于NW。OW的CAT和SOD浓度高于TW。在TW和OW中,APX的活性均高于GPX。除机械应力外,其他气候因素对活性氧积累也有影响。
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引用次数: 0
Evaluating Carlquist’s Law from a physiological perspective 从生理学角度评价卡尔奎斯特定律
IF 1.9 3区 农林科学 Q2 FORESTRY Pub Date : 2023-08-15 DOI: 10.1163/22941932-bja10134
Kate M. Johnson, Sophie R. Everbach, N. Holbrook, M. Olson
“Carlquist’s Law” is a striking pattern of association between anatomical features in the wood of vessel-bearing plants. It derives from Sherwin Carlquist’s observation that xylem vessels tend to be solitary when embedded in a matrix of imperforate tracheary elements that appear to be conductive, whereas xylem vessels tend to be grouped when surrounded by seemingly non-conductive cells. Vessel-vessel contacts (vessel grouping) allow water to travel between conduits, but also provide pathways for air to propagate from embolized (air-filled) vessels into functional vessels. If the background matrix is conductive, it is conceivable that water could bypass embolized vessels, providing an alternative transport route in species with conductive backgrounds and solitary vessels. Much remains to be tested in this hypothesis, including the topology of the vessel networks in species with solitary versus grouped vessels and how conductive the different imperforate tracheary element types are. Exploring Carlquist’s Law promises to provide key insight into the causes of embolism in plant conduits, the modes of embolism passage between conduits, and how vessels and the cells in which they are imbedded may interact to govern the pathways of water flow through plants.
“卡尔奎斯特定律”是一种引人注目的血管植物木材解剖特征之间的联系模式。这源于Sherwin Carlquist的观察,即当木质部导管嵌入似乎具有导电性的无孔管状元体基质中时,木质部导管往往是孤立的,而当木质部血管被看似不导电的细胞包围时,它们往往是成组的。血管-血管接触(血管分组)允许水在导管之间流动,但也为空气从栓塞(充气)血管传播到功能性血管提供了途径。如果背景基质是导电的,可以想象水可以绕过栓塞的血管,为具有导电背景和孤立血管的物种提供一种替代的运输途径。在这一假设中还有很多有待检验的地方,包括具有单独血管和成组血管的物种的血管网络拓扑结构,以及不同的无孔气管元件类型的导电性。探索Carlquist定律有望为深入了解植物导管中栓塞的原因、导管之间栓塞的通道模式,以及血管和它们所嵌入的细胞如何相互作用以控制植物中的水流路径提供关键见解。
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引用次数: 0
Exploring monsoon precipitation signal in the Peruvian tropical montane cloud forest: Andean Walnut wood anatomy plasticity 探索秘鲁热带山地云雾林季风降水信号:安第斯胡桃木解剖可塑性
IF 1.9 3区 农林科学 Q2 FORESTRY Pub Date : 2023-07-28 DOI: 10.1163/22941932-bja10132
Ernesto C. Rodríguez-Ramírez, Doris B. Crispin‐DelaCruz, M. Morales, G. Ticse-Otarola, F. N. Ames-Martínez, Anthony Guerra, Cassiana Alves-Ferreira, Edilson J. Requena-Rojas
Andean Walnut (Juglans neotropica Diels-Juglandaceae) is a long-lived, deciduous broadleaf Tropical Montane Cloud Forest (TMCF) tree species native to the Andes Cordillera; nevertheless, it has received limited attention for dendro-quantitative wood anatomical studies. Based on 70 increment cores from 50 Andean Walnut trees at two Peruvian TMCFs, two chronologies (from 1969 to 2020 and from 1964 to 2020) were developed. The xylem vessel parameters assessment allowed us to detect South American Monsoon System (SAMS) precipitation signals in the Andean Walnuts’ wood. Dendro-wood anatomical features can be assessed within an annual growth ring, which allows for assessing intra-annual past and present wood anatomy-function relationships and its climate vulnerability.
安第斯核桃(核桃科)是一种长寿命,落叶阔叶热带山地云雾林(TMCF)树种,原产于安第斯山脉;然而,在树突定量木材解剖研究中受到的关注有限。基于两个秘鲁tmcf的50棵安第斯核桃树的70个增量岩心,开发了两个年表(1969 - 2020年和1964 - 2020年)。木质部导管参数评估使我们能够在安第斯核桃木中探测到南美季风系统(SAMS)降水信号。树木解剖特征可以在年轮内进行评估,从而可以评估过去和现在的年度木材解剖功能关系及其气候脆弱性。
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引用次数: 0
Anatomical and isotopic traits in grapevine wood rings record environmental variability 葡萄藤木环的解剖和同位素特征记录了环境变异
IF 1.9 3区 农林科学 Q2 FORESTRY Pub Date : 2023-07-11 DOI: 10.1163/22941932-bja10131
N. Damiano, G. Battipaglia, P. Cherubini, C. Amitrano, S. Altieri, L. Schneider, A. Balzano, C. Cirillo, Veronica De Micco
In the Mediterranean region, prolonged droughts affect the growth and reproductive cycles of grapevine. Changes in the physiological processes of grapevine, consequent to variations in environmental factors or cultivation management, are recorded in wood anatomical and isotopic traits in grapevine stems. In this study, we measured the anatomical traits and stable carbon isotope content in the annual rings of Vitis vinifera L. subsp. vinifera ‘Falanghina’ in four vineyards located in southern Italy, characterised by different water availability. The aim was to investigate how wood anatomical traits respond to interannual climatic variations according to local conditions. Wood cores were taken from the stem of the grapevines and subjected to both microscopy and carbon stable isotope analyses to quantify functional wood anatomical traits, such as vessel size and frequency, and the intrinsic water-use efficiency of the grapevine. Wood traits were correlated with data on precipitation and temperature. The results showed that the plants at the four vineyards were characterised by differences in wood structure influencing the grapevine’s physiology under different conditions of water availability. Overall, the analyses showed that the grapevines at the wetter sites developed wood traits, e.g., wide vessels, which favour the efficiency of water flow, while at the drier sites, they developed plant traits, e.g., small vessels, which favour safety against embolism. However, the robustness of such main trends is trait-specific and is influenced by interannual climatic variability.
在地中海地区,长期干旱影响葡萄藤的生长和繁殖周期。由于环境因素或栽培管理的变化,葡萄藤生理过程的变化记录在葡萄藤茎的木材解剖和同位素特征中。本研究对葡萄年轮的解剖特征和稳定碳同位素含量进行了测定。Falanghina葡萄园位于意大利南部的四个葡萄园,以不同的水资源供应为特征。目的是根据当地条件调查木材解剖特征如何响应年际气候变化。从葡萄藤茎中提取木芯,并进行显微镜和碳稳定同位素分析,以量化木材的功能解剖特征,如血管大小和频率,以及葡萄藤的内在水分利用效率。木材性状与降水和温度相关。结果表明,在不同的水分条件下,四个葡萄园的植物在木材结构上的差异影响了葡萄的生理机能。总体而言,分析表明,湿润地点的葡萄藤发展出木材特征,例如,宽血管,有利于水流效率,而在干燥地点,葡萄藤发展出植物特征,例如,小血管,有利于安全防止栓塞。然而,这些主要趋势的稳健性是性状特有的,并受到年际气候变率的影响。
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引用次数: 0
A technique for high-density wood softening in the micro-sectioning process for wood anatomy studies 用于木材解剖研究的微切片过程中的高密度木材软化技术
IF 1.9 3区 农林科学 Q2 FORESTRY Pub Date : 2023-06-27 DOI: 10.1163/22941932-bja10130
Riya Tudu Solanki
Wood identification for commercial applications is important and has a significant demand worldwide. Microscopic wood identification involves many processes such as softening, sectioning, and data analysis. Softening of wood is a crucial method for obtaining good sections for microscopic analysis but softening high-density wood is difficult. An alternate method of softening high-density wood for wood identification is presented. Different concentrations of alkali, NaOH, were tried, and optimized for different treatment times to obtain adequate softening of high-density wood. 20% NaOH treatment for 5 days at 30°C was used to soften high-density wood of variable density between 1 and 1.118 g/cm3. The species studied were Calophyllum spp., Shorea robusta, and Handroanthus spp., in which softening was satisfactory.
木材识别的商业应用是重要的,并有显著的世界范围内的需求。木材显微鉴定涉及许多过程,如软化、切片和数据分析。木材的软化是获得良好的微观分析截面的关键方法,但软化高密度木材是困难的。提出了一种用于木材识别的软化高密度木材的替代方法。试验了不同浓度的NaOH碱,并优化了不同的处理时间,以获得足够的软化高密度木材。采用20% NaOH在30℃下处理5天,软化密度在1 ~ 1.118 g/cm3之间的高密度木材。研究的种类有Calophyllum spp、Shorea robusta和Handroanthus spp,软化程度令人满意。
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引用次数: 1
Walter Liese (1926–2023) 沃尔特-利斯(1926-2023)
3区 农林科学 Q2 FORESTRY Pub Date : 2023-06-21 DOI: 10.1163/22941932-00002249
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引用次数: 0
Paleocene fossil wood from Patagonia with storied rays and comments on the fossil record of this character 巴塔哥尼亚古新世木化石及其化石记录评述
IF 1.9 3区 农林科学 Q2 FORESTRY Pub Date : 2023-06-01 DOI: 10.1163/22941932-bja10129
Daniela P. Ruiz, Roberto R. Pujana, M. Brea
The storied structure in wood anatomy is considered derived and highly specialized, and is present in some related families. The first records of this character are from the Cretaceous, but it is scarce in the Cretaceous and Paleocene, and apparently absent until the Eocene in the temperate Southern Hemisphere. Using standard methodology, we describe the anatomy of a fossil wood from the early Paleocene of Patagonia (Salamanca Formation). The fossil wood, assigned to a new genus and species Elizabethiaxylon patagonicum related to the Malvaceae, is characterized by its diffuse-porous wood, solitary vessels, simple perforation plates, apotracheal banded axial parenchyma, and mostly biseriate storied rays. The storied structure in this wood is one of the oldest records from Gondwana.
木材解剖学中的分层结构被认为是衍生的和高度专业化的,并且存在于一些相关的家族中。这种特征的第一次记录来自白垩纪,但在白垩纪和古新世时很少,在温带南半球,直到始新世才明显存在。使用标准方法,我们描述了巴塔哥尼亚古新世早期(萨拉曼卡组)的木材化石的解剖结构。该化石木材属于锦葵科的一个新属和一个新种伊丽莎白木巴塔哥尼亚木,其特征是其木材具有弥漫的多孔性、孤立的血管、简单的穿孔板、离气管的带状轴向薄壁组织和多为双列层射线。这块木头上的分层结构是冈瓦纳大陆最古老的记录之一。
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引用次数: 0
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