Insulin-like growth factors (IGFs) act as a critical signaling pathway in animals and play significant roles in body growth, development, and occurrence and progression of animal diseases. In the past, structural and functional studies of ligands, receptors and even specific binding proteins in the IGFs system have been extensively investigated. However, the functional study of insulin-like growth factor acid-labile subunit (IGFALS) mainly focused on the prolonging half-life of IGFs. Increasing number of studies indicated that mutations in the IGFALS DNA sequence and low expression level of IGFALS proteins can lead to growth and development retardation in animals. In this review, we summarize recent structural and functional studies of IGFALS in mammals, aiming to further identify detailed genetic mechanism of IGFALS.
{"title":"[Structure and function of insulin-like growth factor acid-labile subunits in mammalian homologues].","authors":"L. Guilin, N. Lili, L. Haifeng, G. Jia-zhong","doi":"10.16288/J.YCZZ.15-192","DOIUrl":"https://doi.org/10.16288/J.YCZZ.15-192","url":null,"abstract":"Insulin-like growth factors (IGFs) act as a critical signaling pathway in animals and play significant roles in body growth, development, and occurrence and progression of animal diseases. In the past, structural and functional studies of ligands, receptors and even specific binding proteins in the IGFs system have been extensively investigated. However, the functional study of insulin-like growth factor acid-labile subunit (IGFALS) mainly focused on the prolonging half-life of IGFs. Increasing number of studies indicated that mutations in the IGFALS DNA sequence and low expression level of IGFALS proteins can lead to growth and development retardation in animals. In this review, we summarize recent structural and functional studies of IGFALS in mammals, aiming to further identify detailed genetic mechanism of IGFALS.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"37 1","pages":"1185-1193"},"PeriodicalIF":2.7,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Jinwei, Luo Yi, W. Yuhao, H. Liujun, L. Mingzhou, Wang Xun
Adipose tissues play a critical role in the regulation of energy metabolism and homeostasis, and is also an important endocrine organ. Adipocyte differentiation is a complicated physiological process during which mesenchymal stem cells differentiate into adipocytes. This process is synergistically regulated by a large number of transcription factors, hormones and signaling pathway molecules. As a class of endogenous non-coding RNA (ncRNA), microRNAs (miRNAs) regulate gene expression mainly through post-transcriptional translational repression. In recent years, numerous studies have demonstrated that miRNA could have an impact on adipocyte differentiation and adipogenesis by modulating the expression levels of several adipogenic transcription factors and key signaling molecules. In this review, we summarize the mechanism of miRNA in regulating the differentiation of white/brown/beige adipocytes and the relevant signaling pathways and key factors, in the hope of providing theoretical guidance and new thoughts for treating obesity and other metabolic diseases.
{"title":"MicroRNA regulates animal adipocyte differentiation","authors":"Z. Jinwei, Luo Yi, W. Yuhao, H. Liujun, L. Mingzhou, Wang Xun","doi":"10.16288/J.YCZZ.15-233","DOIUrl":"https://doi.org/10.16288/J.YCZZ.15-233","url":null,"abstract":"Adipose tissues play a critical role in the regulation of energy metabolism and homeostasis, and is also an important endocrine organ. Adipocyte differentiation is a complicated physiological process during which mesenchymal stem cells differentiate into adipocytes. This process is synergistically regulated by a large number of transcription factors, hormones and signaling pathway molecules. As a class of endogenous non-coding RNA (ncRNA), microRNAs (miRNAs) regulate gene expression mainly through post-transcriptional translational repression. In recent years, numerous studies have demonstrated that miRNA could have an impact on adipocyte differentiation and adipogenesis by modulating the expression levels of several adipogenic transcription factors and key signaling molecules. In this review, we summarize the mechanism of miRNA in regulating the differentiation of white/brown/beige adipocytes and the relevant signaling pathways and key factors, in the hope of providing theoretical guidance and new thoughts for treating obesity and other metabolic diseases.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"37 1","pages":"1175-1184"},"PeriodicalIF":2.7,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-11eCollection Date: 2015-01-01DOI: 10.3897/zookeys.534.6169
Fábio F Roxo, Gabriel S C Silva, Luz E Ochoa, Claudio Oliveira
The genus Hisonotus was resurrected as a member of the tribe Otothyrini (actually subfamily Otothyrinae). However, phylogenetic studies based on morphological and molecular data showed that Hisonotus is not monophyletic and independent lineages can be identified, such as the group composed of the species Hisonotus insperatus, Hisonotus luteofrenatus, Hisonotus oliveirai, Hisonotus paresi and Hisonotus piracanjuba, a lineage unrelated to that containing the type species of the genus Hisonotus (Hisonotus notatus). Herein, based in molecular and morphological data, a new genus is described to accommodate the lineage mentioned above, into which are also added three new species. This new genus can be distinguished from other genera of Otothyrinae by the following combination of characters: (1) a pair of rostral plates at the tip of the snout; (2) two large pre-nasal plates just posterior to the rostral plates; (3) a supra-opercular plate that receives the laterosensory canal from the compound pterotic before the preopercle; (4) a well developed membrane at anal opening in females; and (5) a V-shaped spinelet. A key to species of Curculionichthys is provided.
{"title":"Description of a new genus and three new species of Otothyrinae (Siluriformes, Loricariidae).","authors":"Fábio F Roxo, Gabriel S C Silva, Luz E Ochoa, Claudio Oliveira","doi":"10.3897/zookeys.534.6169","DOIUrl":"10.3897/zookeys.534.6169","url":null,"abstract":"<p><p>The genus Hisonotus was resurrected as a member of the tribe Otothyrini (actually subfamily Otothyrinae). However, phylogenetic studies based on morphological and molecular data showed that Hisonotus is not monophyletic and independent lineages can be identified, such as the group composed of the species Hisonotus insperatus, Hisonotus luteofrenatus, Hisonotus oliveirai, Hisonotus paresi and Hisonotus piracanjuba, a lineage unrelated to that containing the type species of the genus Hisonotus (Hisonotus notatus). Herein, based in molecular and morphological data, a new genus is described to accommodate the lineage mentioned above, into which are also added three new species. This new genus can be distinguished from other genera of Otothyrinae by the following combination of characters: (1) a pair of rostral plates at the tip of the snout; (2) two large pre-nasal plates just posterior to the rostral plates; (3) a supra-opercular plate that receives the laterosensory canal from the compound pterotic before the preopercle; (4) a well developed membrane at anal opening in females; and (5) a V-shaped spinelet. A key to species of Curculionichthys is provided. </p>","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"40 1","pages":"103-34"},"PeriodicalIF":1.3,"publicationDate":"2015-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85416835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sheng-song Xie, Yi Zhang, Li-sheng Zhang, Guang-lei Li, Chang-zhi Zhao, Pan Ni, Shu-hong Zhao
The third generation of CRISPR/Cas9-mediated genome editing technology has been successfully applied to genome modification of various species including animals, plants and microorganisms. How to improve the efficiency of CRISPR/Cas9 genome editing and reduce its off-target effects has been extensively explored in this field. Using sgRNA (Small guide RNA) with high efficiency and specificity is one of the critical factors for successful genome editing. Several software have been developed for sgRNA design and/or off-target evaluation, which have advantages and disadvantages respectively. In this review, we summarize characters of 16 kinds online and standalone software for sgRNA design and/or off-target evaluation and conduct a comparative analysis of these different kinds of software through developing 38 evaluation indexes. We also summarize 11 experimental approaches for testing genome editing efficiency and off-target effects as well as how to screen highly efficient and specific sgRNA.
{"title":"[sgRNA design for the CRISPR/Cas9 system and evaluation of its off-target effects].","authors":"Sheng-song Xie, Yi Zhang, Li-sheng Zhang, Guang-lei Li, Chang-zhi Zhao, Pan Ni, Shu-hong Zhao","doi":"10.16288/j.yczz.15-093","DOIUrl":"10.16288/j.yczz.15-093","url":null,"abstract":"<p><p>The third generation of CRISPR/Cas9-mediated genome editing technology has been successfully applied to genome modification of various species including animals, plants and microorganisms. How to improve the efficiency of CRISPR/Cas9 genome editing and reduce its off-target effects has been extensively explored in this field. Using sgRNA (Small guide RNA) with high efficiency and specificity is one of the critical factors for successful genome editing. Several software have been developed for sgRNA design and/or off-target evaluation, which have advantages and disadvantages respectively. In this review, we summarize characters of 16 kinds online and standalone software for sgRNA design and/or off-target evaluation and conduct a comparative analysis of these different kinds of software through developing 38 evaluation indexes. We also summarize 11 experimental approaches for testing genome editing efficiency and off-target effects as well as how to screen highly efficient and specific sgRNA.</p>","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"37 11 1","pages":"1125-36"},"PeriodicalIF":2.7,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.16288/j.yczz.15-093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the rapid development of molecular biology technology, researchers have got much deeper understanding of the role of long non-coding RNA (lncRNA). It is not only indispensable for biological processes, but also plays an important role in human diseases especially in tumor. Previous studies have shown that a variety of lncRNAs are closely associated with hematologic malignancies. These lncRNAs are involved in diseases through diverse functions including affecting the expression of tumor suppressor gene p15, regulating p53 protein function, and interacting with miRNAs. In this review, we summarize the hematological tumor-associated lncRNAs and focus on p15, p53 and miRNA-related lncRNAs as well as the role of their interaction in hematological malignancies, which may provide a comprehensive understanding of the role of hematological tumor-associated lncRNAs and some insights for research, diagnosis and treatment of hematologic malignancies.
{"title":"[The role of long non-coding RNAs in hematologic malignancies].","authors":"H. Wanli, G. Ai","doi":"10.16288/J.YCZZ.15-261","DOIUrl":"https://doi.org/10.16288/J.YCZZ.15-261","url":null,"abstract":"With the rapid development of molecular biology technology, researchers have got much deeper understanding of the role of long non-coding RNA (lncRNA). It is not only indispensable for biological processes, but also plays an important role in human diseases especially in tumor. Previous studies have shown that a variety of lncRNAs are closely associated with hematologic malignancies. These lncRNAs are involved in diseases through diverse functions including affecting the expression of tumor suppressor gene p15, regulating p53 protein function, and interacting with miRNAs. In this review, we summarize the hematological tumor-associated lncRNAs and focus on p15, p53 and miRNA-related lncRNAs as well as the role of their interaction in hematological malignancies, which may provide a comprehensive understanding of the role of hematological tumor-associated lncRNAs and some insights for research, diagnosis and treatment of hematologic malignancies.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"37 1","pages":"1095-1104"},"PeriodicalIF":2.7,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xin Liu, Xueying Song, Xiaoping Zhang, Ying-lun Han, Ting Zhu, R. Xiao, Qing-wei Li
In recent years, the antigen recognition mechanism based on variable lymphocyte receptors (VLRs) was found in agnathan lamprey. To illuminate the genetic basis of immune response of lymphocyte-like cells in the mucosal immune system of lamprey and explore the evolutionary relationship of adaptive immune responses between the jawless and jawed vertebrates, we constructed cDNA libraries of lamprey (Lampetra japonica) gills before and after stimulation, and then performed high-throughput transcriptome sequencing and analysis. Through functional annotation of 88 525 assembled unigenes, 21 704 and 9769 unigenes were annotated in Gene Ontology (GO) and Kyto Encyclopedia of Genes and Genomes (KEGG) databases, respectively. Among 999 unigenes involved in multiple pathways of immune system, 184 unigenes were highly homologous to 51 TCR (T cell receptor) and BCR (B cell receptor) signalling molecules in higher vertebrates, indicating that molecules involved in adaptive immune signalling pathways in higher vertebrates also exist in lampreys. In addition, identification of five VLRA, seven VLRB and four VLRC molecules suggest that at least three types of lymphocyte subsets are distributed in lamprey gill mucosal immune tissues. The results of real-time fluorescence quantitative PCR showed that the expression levels of Lck, Fyn and Zap70 were up-regulated after immune stimulation while those of Syk, Btk and Blnk were not changed significantly, indicating the activation of TCR-like signal transduction pathway after antigen stimulation in lamprey gill tissues. Our studies preliminaryly proved that two parallel adaptive immune systems in jawless and jawed vertebrates have common genetic basis, and also provided valuable clues to the exploration of signalling processes of VLRA⁺, VLRB⁺, and VLRC⁺ lymphocyte-like cells in response to antigens.
近年来在七鳃鳗中发现了基于可变淋巴细胞受体(VLRs)的抗原识别机制。为了阐明七鳃鳗粘膜免疫系统中淋巴细胞样细胞免疫应答的遗传基础，探索无颌和有颌脊椎动物之间适应性免疫应答的进化关系，我们构建了刺激前后七鳃鳗(Lampetra japonica)鳃的cDNA文库，并进行了高通量转录组测序和分析。通过对88 525个组装的unigenes进行功能标注，分别在Gene Ontology (GO)和Kyto Encyclopedia of Genes and Genomes (KEGG)数据库中标注了21 704个和9769个unigenes。在参与免疫系统多通路的999个unique igenes中，184个unique igenes与51个高等脊椎动物的TCR (T细胞受体)和BCR (B细胞受体)信号分子高度同源，说明参与高等脊椎动物适应性免疫信号通路的分子在七鳃鳗中也存在。此外，5个VLRA、7个VLRB和4个VLRC分子的鉴定表明，在七鳃鳗鳃黏膜免疫组织中至少分布着3种类型的淋巴细胞亚群。实时荧光定量PCR结果显示，免疫刺激后Lck、Fyn和Zap70的表达水平上调，而Syk、Btk和Blnk的表达水平变化不明显，表明抗原刺激后七鳃鱼鳃组织中tcr样信号转导通路被激活。我们的研究初步证明了无颌和有颌脊椎动物两种平行的适应性免疫系统具有共同的遗传基础，也为探索VLRA +、VLRB +、VLRC +淋巴细胞样细胞对抗原反应的信号传导过程提供了有价值的线索。
{"title":"[Genetic basis of immune response of lymphocyte-like cells in the mucosal immune system of Lampetra japonica].","authors":"Xin Liu, Xueying Song, Xiaoping Zhang, Ying-lun Han, Ting Zhu, R. Xiao, Qing-wei Li","doi":"10.16288/j.yczz.15-108","DOIUrl":"https://doi.org/10.16288/j.yczz.15-108","url":null,"abstract":"In recent years, the antigen recognition mechanism based on variable lymphocyte receptors (VLRs) was found in agnathan lamprey. To illuminate the genetic basis of immune response of lymphocyte-like cells in the mucosal immune system of lamprey and explore the evolutionary relationship of adaptive immune responses between the jawless and jawed vertebrates, we constructed cDNA libraries of lamprey (Lampetra japonica) gills before and after stimulation, and then performed high-throughput transcriptome sequencing and analysis. Through functional annotation of 88 525 assembled unigenes, 21 704 and 9769 unigenes were annotated in Gene Ontology (GO) and Kyto Encyclopedia of Genes and Genomes (KEGG) databases, respectively. Among 999 unigenes involved in multiple pathways of immune system, 184 unigenes were highly homologous to 51 TCR (T cell receptor) and BCR (B cell receptor) signalling molecules in higher vertebrates, indicating that molecules involved in adaptive immune signalling pathways in higher vertebrates also exist in lampreys. In addition, identification of five VLRA, seven VLRB and four VLRC molecules suggest that at least three types of lymphocyte subsets are distributed in lamprey gill mucosal immune tissues. The results of real-time fluorescence quantitative PCR showed that the expression levels of Lck, Fyn and Zap70 were up-regulated after immune stimulation while those of Syk, Btk and Blnk were not changed significantly, indicating the activation of TCR-like signal transduction pathway after antigen stimulation in lamprey gill tissues. Our studies preliminaryly proved that two parallel adaptive immune systems in jawless and jawed vertebrates have common genetic basis, and also provided valuable clues to the exploration of signalling processes of VLRA⁺, VLRB⁺, and VLRC⁺ lymphocyte-like cells in response to antigens.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"37 11 1","pages":"1149-59"},"PeriodicalIF":2.7,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-22DOI: 10.1186/s41065-015-0003-8
S. Baumgartner
{"title":"Resurrection of Hereditas, a journal with almost 100 years of tradition","authors":"S. Baumgartner","doi":"10.1186/s41065-015-0003-8","DOIUrl":"https://doi.org/10.1186/s41065-015-0003-8","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"152 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2015-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-015-0003-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65773898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-22DOI: 10.1186/s41065-015-0002-9
Awatef Essid, F. Aljane, A. Ferchichi, J. Hormaza
{"title":"Analysis of genetic diversity of Tunisian caprifig (Ficus carica L.) accessions using simple sequence repeat (SSR) markers","authors":"Awatef Essid, F. Aljane, A. Ferchichi, J. Hormaza","doi":"10.1186/s41065-015-0002-9","DOIUrl":"https://doi.org/10.1186/s41065-015-0002-9","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"152 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2015-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-015-0002-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65773877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-22DOI: 10.1186/s41065-015-0004-7
David Ruiz-Ramos, Yazmín Hernández-Díaz, C. Tovilla-Zárate, I. Juárez-Rojop, M. López-Narváez, T. González-Castro, M. E. Torres-Hernández, M. Baños-González
{"title":"The Trp719Arg polymorphism of the KIF6 gene and coronary heart disease risk: systematic review and meta-analysis","authors":"David Ruiz-Ramos, Yazmín Hernández-Díaz, C. Tovilla-Zárate, I. Juárez-Rojop, M. López-Narváez, T. González-Castro, M. E. Torres-Hernández, M. Baños-González","doi":"10.1186/s41065-015-0004-7","DOIUrl":"https://doi.org/10.1186/s41065-015-0004-7","url":null,"abstract":"","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"152 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2015-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41065-015-0004-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65773916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Yue, L. Xiaodong, D. Yongmei, X. Zongming, Shou-yi Chen
Trihelix transcription factors are important proteins involved in response to abiotic stresses in plants. Understanding the molecular mechanisms of Trihelix in cottons will lay the foundation to improve stress tolerance by gene engineering. In this study, a gene encoding Trihelix transcription factor was isolated in upland cottons using reverse transcription PCR according to bioinformatic analysis. The gene was named as GhGT29 (GenBank accession No. JQ013097), which was 1 092 bp, contained a 1 089 bp open reading frame and encoded a protein of 363 amino acids with a predicted molecular weight of 40.9 kDa and a isoelectric point of 5.45. SMART analysis showed GhGT29 contained one typical SANT motif. Phylogenetic analysis showed that GhGT29 belonged to the SH4 subfamily of the Trihelix family and was most closely related to AtSH4-like1 and AtSH4-like2. Quantitative real-time PCR (qRT-PCR) analysis revealed that GhGT29 was induced by high salt, drought, cold and abscisic acid. The expression profile also revealed that GhGT29 was constitutively expressed in all tested tissues, such as roots, stems, leaves, flowers, ovules (0 DPA) and fibers (12 DPA). The expression level of GhGT29 was the highest in flowers and the lowest in stems. Using the Arabidopsis protoplasts assay system, we found that the GhGT29 protein was located in cell nuclei and had trans-activation activity. These results revealed that GhGT29 might be involved in the regulation of stress resistance-related genes in stress signaling pathways in upland cottons.
{"title":"Cloning and functional analysis of the cotton Trihelix transcription factor GhGT29","authors":"L. Yue, L. Xiaodong, D. Yongmei, X. Zongming, Shou-yi Chen","doi":"10.16288/J.YCZZ.15-320","DOIUrl":"https://doi.org/10.16288/J.YCZZ.15-320","url":null,"abstract":"Trihelix transcription factors are important proteins involved in response to abiotic stresses in plants. Understanding the molecular mechanisms of Trihelix in cottons will lay the foundation to improve stress tolerance by gene engineering. In this study, a gene encoding Trihelix transcription factor was isolated in upland cottons using reverse transcription PCR according to bioinformatic analysis. The gene was named as GhGT29 (GenBank accession No. JQ013097), which was 1 092 bp, contained a 1 089 bp open reading frame and encoded a protein of 363 amino acids with a predicted molecular weight of 40.9 kDa and a isoelectric point of 5.45. SMART analysis showed GhGT29 contained one typical SANT motif. Phylogenetic analysis showed that GhGT29 belonged to the SH4 subfamily of the Trihelix family and was most closely related to AtSH4-like1 and AtSH4-like2. Quantitative real-time PCR (qRT-PCR) analysis revealed that GhGT29 was induced by high salt, drought, cold and abscisic acid. The expression profile also revealed that GhGT29 was constitutively expressed in all tested tissues, such as roots, stems, leaves, flowers, ovules (0 DPA) and fibers (12 DPA). The expression level of GhGT29 was the highest in flowers and the lowest in stems. Using the Arabidopsis protoplasts assay system, we found that the GhGT29 protein was located in cell nuclei and had trans-activation activity. These results revealed that GhGT29 might be involved in the regulation of stress resistance-related genes in stress signaling pathways in upland cottons.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"37 1","pages":"1218-1227"},"PeriodicalIF":2.7,"publicationDate":"2015-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：