首页 > 最新文献

Folia Parasitologica最新文献

英文 中文
Trichinellosis in Serbia has become a rare event - one outbreak with pulmonary complications. 在塞尔维亚,旋毛虫已经成为一种罕见的事件——一次爆发时伴有肺部并发症。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-04-06 DOI: 10.14411/fp.2022.009
I. Mitic, S. Vasilev, M. Korac, N. Ilić, Biljana Bojić, A. Gruden-Movsesijan, Ljiljana Sofronić-Milosavljević
An outbreak of trichinellosis occurred in Stari Banovci, a settlement in the municipality of Stara Pazova, Srem, Republic of Serbia, in March-April 2019. A total of 28 persons were exposed and trichinellosis was confirmed in 24 of them. This outbreak involved members of eight families, their relatives and friends. The infection, due to Trichinella spiralis (Owen, 1835), was associated with consumption of pork sausages procured in a local butcher's shop. The trace-back study revealed that the meat originated from swine that was raised on a small farm belonging to the owner of the shop, who did not have permission from the Veterinary Directorate for slaughtering animals and who put on the market sausages prepared from uninspected meat. Trichinellosis was accompanied by typical clinical symptoms. However, the unusual occurrence of high percentage of pulmonary complications was noticed. The description of this outbreak indicates that medical practitioners should initiate treatment immediately in cases of high suspicion of trichinellosis, even if the serology is negative, to prevent the complications of the disease. In spite of significant achievements in the control of Trichinella infection among domestic pigs and humans in the last 10 years, it is obvious that such cases of breeding animals under inappropriate conditions, slaughtering them without approval and placing uninspected pork on the market represent a source of sporadic outbreaks in Serbia.
2019年3月至4月,塞尔维亚共和国斯雷姆市斯塔拉帕佐瓦市的斯塔里巴诺维奇村暴发了旋毛虫病。共有28人接触,其中24人确诊旋毛虫病。这次疫情涉及八个家庭成员及其亲戚和朋友。由旋毛虫引起的感染(Owen, 1835年)与食用在当地肉店采购的猪肉香肠有关。追溯研究显示,这些肉来自该商店老板的一个小农场饲养的猪,该店主没有获得兽医局屠宰动物的许可,并将未经检查的肉制成的香肠投放市场。旋毛虫病伴有典型的临床症状。然而,注意到肺部并发症的异常发生率很高。对这次暴发的描述表明,即使血清学结果为阴性,医生也应在高度怀疑旋毛虫病的病例中立即开始治疗,以防止该病的并发症。尽管在过去10年中在控制家猪和人之间的旋毛虫感染方面取得了重大成就,但很明显,在不适当的条件下饲养动物、未经批准屠宰和将未经检查的猪肉投放市场的情况是塞尔维亚零星暴发的一个来源。
{"title":"Trichinellosis in Serbia has become a rare event - one outbreak with pulmonary complications.","authors":"I. Mitic, S. Vasilev, M. Korac, N. Ilić, Biljana Bojić, A. Gruden-Movsesijan, Ljiljana Sofronić-Milosavljević","doi":"10.14411/fp.2022.009","DOIUrl":"https://doi.org/10.14411/fp.2022.009","url":null,"abstract":"An outbreak of trichinellosis occurred in Stari Banovci, a settlement in the municipality of Stara Pazova, Srem, Republic of Serbia, in March-April 2019. A total of 28 persons were exposed and trichinellosis was confirmed in 24 of them. This outbreak involved members of eight families, their relatives and friends. The infection, due to Trichinella spiralis (Owen, 1835), was associated with consumption of pork sausages procured in a local butcher's shop. The trace-back study revealed that the meat originated from swine that was raised on a small farm belonging to the owner of the shop, who did not have permission from the Veterinary Directorate for slaughtering animals and who put on the market sausages prepared from uninspected meat. Trichinellosis was accompanied by typical clinical symptoms. However, the unusual occurrence of high percentage of pulmonary complications was noticed. The description of this outbreak indicates that medical practitioners should initiate treatment immediately in cases of high suspicion of trichinellosis, even if the serology is negative, to prevent the complications of the disease. In spite of significant achievements in the control of Trichinella infection among domestic pigs and humans in the last 10 years, it is obvious that such cases of breeding animals under inappropriate conditions, slaughtering them without approval and placing uninspected pork on the market represent a source of sporadic outbreaks in Serbia.","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42070919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Association between infection with Toxoplasma gondii and psychiatric disorders. 刚地弓形虫感染与精神疾病的关系。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-04-04 DOI: 10.14411/fp.2022.008
A. Maisarah, Suharni Mohamad, Maruzairi Husain, Sarimah Abdullah, R. Noordin
Toxoplasmosis is one of the world's most prevalent zoonoses. The causative agent, Toxoplasma gondii (Nicolle et Manceaux, 1908) is a facultative heteroxenic, polyxenic apicomplexan protist. There are several potential pathways of transmission within and between host species. Most infections with T. gondii result from close contact with pets/cats, ingestion of tissue cysts in undercooked meat of infected animals, and oocysts from food or water contaminated by feline faeces. Recently, epidemiological studies have shown that T. gondii infection plays a prominent role in the pathogenesis of several psychiatric disorders. This report reviews the association between T. gondii infection and patients with psychiatric disorders, particularly schizophrenia, depressive disorders and bipolar disorders.
弓形虫病是世界上最流行的人畜共患病之一。病原体,刚地弓形虫(Nicolle et Manceaux, 1908)是一种兼性异源、多源的顶复合体原生生物。在宿主物种内部和物种之间有几种潜在的传播途径。大多数弓形虫感染是由于与宠物/猫密切接触、食用受感染动物未煮熟的肉中的组织囊肿,以及从被猫粪便污染的食物或水中获得卵囊造成的。近年来,流行病学研究表明,弓形虫感染在多种精神疾病的发病机制中起着突出作用。本报告综述了弓形虫感染与精神疾病,特别是精神分裂症、抑郁症和双相情感障碍患者之间的关系。
{"title":"Association between infection with Toxoplasma gondii and psychiatric disorders.","authors":"A. Maisarah, Suharni Mohamad, Maruzairi Husain, Sarimah Abdullah, R. Noordin","doi":"10.14411/fp.2022.008","DOIUrl":"https://doi.org/10.14411/fp.2022.008","url":null,"abstract":"Toxoplasmosis is one of the world's most prevalent zoonoses. The causative agent, Toxoplasma gondii (Nicolle et Manceaux, 1908) is a facultative heteroxenic, polyxenic apicomplexan protist. There are several potential pathways of transmission within and between host species. Most infections with T. gondii result from close contact with pets/cats, ingestion of tissue cysts in undercooked meat of infected animals, and oocysts from food or water contaminated by feline faeces. Recently, epidemiological studies have shown that T. gondii infection plays a prominent role in the pathogenesis of several psychiatric disorders. This report reviews the association between T. gondii infection and patients with psychiatric disorders, particularly schizophrenia, depressive disorders and bipolar disorders.","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42046576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs. 关于Dirofilaria spp.在捷克共和国狗中分布的进一步数据。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-03-31 DOI: 10.14411/fp.2022.007
J. Juránková, Barbora Šenkyříková Mitková, Marcela Novotná, L. Hofmannová, B. Červená, D. Bowman, D. Modrý
Based on previously published data, the Czech Republic is regarded an endemic country of the onchocercid nematodes Dirofilaria immitis (Leidy, 1856) and Dirofilaria repens Railliet et Henry, 1911. Nevertheless, while cases of D. repens are commonly reported from dogs in South Moravia, no recent records of D. immitis are available. Therefore, the present study was performed to clarify the occurrence of both species of Dirofilaria Railliet et Henry, 1910. Blood samples of 551 dogs sampled during 2015 and 2016 were analysed microscopically for presence of microfilariae and blood sera were examined by IDEXX SNAP® 4Dx® test (IDEXX, USA). DNA from blood of microscopically positive dogs was extracted and PCR protocol amplifying fragment of cytochrome c oxidase I (COI) gene was performed; PCR products were then sequenced. All dogs from the Bohemian part of the Czech Republic were negative. The prevalence of D. repens in the Moravian region was 5.7 % (27/476). BLAST analyses of obtained sequences confirmed the presence of D. repens (99-100% identical to KX265049). All sampled animals showed a negative result for D. immitis antigen in IDEXX SNAP® 4Dx® test. Our study confirmed the previously reported occurrence of D. repens in South Moravia and revealed its spreading from the epicentre to the north and west. PCR with subsequent sequencing together with negative results for D. immitis antigen in IDEXX SNAP® 4Dx® test revealed only D. repens infection. A previously published autochthonous infection of dogs with D. immitis in South Moravia was not confirmed.
根据先前公布的数据,捷克共和国被认为是盘尾丝虫病的流行国家(Leidy, 1856)和repens Dirofilaria Railliet et Henry, 1911)。然而,虽然在南摩拉维亚经常报告狗感染repens的病例,但没有最近的immitis记录。因此,本研究旨在阐明两种Dirofilaria Railliet et Henry, 1910的发生情况。对2015年和2016年采集的551只狗的血样进行显微分析,检测微丝蚴的存在,并采用IDEXX SNAP®4Dx®检测(IDEXX, USA)检测血清。从镜检阳性犬血液中提取DNA,进行细胞色素c氧化酶I (COI)基因PCR扩增;然后对PCR产物进行测序。所有来自捷克共和国波西米亚地区的狗都是阴性的。在摩拉维亚地区流行率为5.7%(27/476)。BLAST分析获得的序列证实了D. repens的存在(与KX265049相同99-100%)。所有动物在IDEXX SNAP®4Dx®检测中均显示D. imimtis抗原阴性。我们的研究证实了先前报道的在南摩拉维亚发生的D. repens,并揭示了其从震中向北部和西部蔓延。随后的PCR测序以及IDEXX SNAP®4Dx®检测中D. immitis抗原阴性结果显示只有D. repens感染。先前在南摩拉维亚发表的犬类原生感染阿米特弓形虫病未得到证实。
{"title":"Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs.","authors":"J. Juránková, Barbora Šenkyříková Mitková, Marcela Novotná, L. Hofmannová, B. Červená, D. Bowman, D. Modrý","doi":"10.14411/fp.2022.007","DOIUrl":"https://doi.org/10.14411/fp.2022.007","url":null,"abstract":"Based on previously published data, the Czech Republic is regarded an endemic country of the onchocercid nematodes Dirofilaria immitis (Leidy, 1856) and Dirofilaria repens Railliet et Henry, 1911. Nevertheless, while cases of D. repens are commonly reported from dogs in South Moravia, no recent records of D. immitis are available. Therefore, the present study was performed to clarify the occurrence of both species of Dirofilaria Railliet et Henry, 1910. Blood samples of 551 dogs sampled during 2015 and 2016 were analysed microscopically for presence of microfilariae and blood sera were examined by IDEXX SNAP® 4Dx® test (IDEXX, USA). DNA from blood of microscopically positive dogs was extracted and PCR protocol amplifying fragment of cytochrome c oxidase I (COI) gene was performed; PCR products were then sequenced. All dogs from the Bohemian part of the Czech Republic were negative. The prevalence of D. repens in the Moravian region was 5.7 % (27/476). BLAST analyses of obtained sequences confirmed the presence of D. repens (99-100% identical to KX265049). All sampled animals showed a negative result for D. immitis antigen in IDEXX SNAP® 4Dx® test. Our study confirmed the previously reported occurrence of D. repens in South Moravia and revealed its spreading from the epicentre to the north and west. PCR with subsequent sequencing together with negative results for D. immitis antigen in IDEXX SNAP® 4Dx® test revealed only D. repens infection. A previously published autochthonous infection of dogs with D. immitis in South Moravia was not confirmed.","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48988447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Intraspecific morphological variation in myxosporeans: high pleomorphic myxospores in the same plasmodium of Myxobolus drjagini (Akhmerov, 1954). 黏液孢子属的种内形态变异:黏液孢子在黏液孢子属同一疟原虫中的高度多形性(Akhmerov, 1954)。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-02-16 DOI: 10.14411/fp.2022.006
Yang Liu, Jiayun Yao, X. Yao, Kaijie Zhang, Qing-peng Wang, Xiaoyi Pan, Jin-Yuan Zhang
The taxonomy of myxosporeans was traditionally dependent solely upon the spore morphological and morphometric data. Intensive reports of intraspecific morphological variation, however, are increasingly challenging the taxonomic approaches for myxosporeans. In the present work, the morphological pleomorphism of myxospores of Myxobolus drjagini (Akhmerov, 1954) was observed. More interestingly, all of these pleomorphic myxospores occurred in the same plasmodium of M. drjagini, which refutes the previous hypothesis that morphological variation of M. drjagini was derived from its responses to differences in nutrition and immunological responses associated with different host tissues. Bearing the intraspecific morphometric and morphotype variation in mind, the combination of morphological, ecological and molecular data should be applied to the species identification and delimitation for myxosporeans. This is the first reported myxobolid species with high pleomorphic myxospores which are present in the same plasmodium.
粘孢子菌的分类传统上仅依赖于孢子形态学和形态计量学数据。然而,关于种内形态变异的大量报道对粘孢子虫的分类方法提出了越来越大的挑战。本研究对Myxobolus drjagini (Akhmerov, 1954)粘液孢子的形态多形性进行了观察。更有趣的是,所有这些多面性黏液孢子都出现在m.d rjagini的同一疟原虫中,这驳斥了先前的假设,即m.d rjagini的形态变化源于其对不同宿主组织的营养差异和免疫反应的反应。考虑到种内形态学和形态型的差异,应结合形态学、生态学和分子学数据进行粘孢子虫的物种鉴定和划界。这是首次报道在同一疟原虫中具有高度多形性黏液孢子的黏液固体种。
{"title":"Intraspecific morphological variation in myxosporeans: high pleomorphic myxospores in the same plasmodium of Myxobolus drjagini (Akhmerov, 1954).","authors":"Yang Liu, Jiayun Yao, X. Yao, Kaijie Zhang, Qing-peng Wang, Xiaoyi Pan, Jin-Yuan Zhang","doi":"10.14411/fp.2022.006","DOIUrl":"https://doi.org/10.14411/fp.2022.006","url":null,"abstract":"The taxonomy of myxosporeans was traditionally dependent solely upon the spore morphological and morphometric data. Intensive reports of intraspecific morphological variation, however, are increasingly challenging the taxonomic approaches for myxosporeans. In the present work, the morphological pleomorphism of myxospores of Myxobolus drjagini (Akhmerov, 1954) was observed. More interestingly, all of these pleomorphic myxospores occurred in the same plasmodium of M. drjagini, which refutes the previous hypothesis that morphological variation of M. drjagini was derived from its responses to differences in nutrition and immunological responses associated with different host tissues. Bearing the intraspecific morphometric and morphotype variation in mind, the combination of morphological, ecological and molecular data should be applied to the species identification and delimitation for myxosporeans. This is the first reported myxobolid species with high pleomorphic myxospores which are present in the same plasmodium.","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47363869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Mitochondrial genome of Rhipicephalus cf. camicasi Morel, Mouchet et Rodhain, 1976 from a camel (Camelus dromedarius Linnaeus) in Riyadh, Saudi Arabia. Morel, Mouchet et Rodhain, 1976年从沙特阿拉伯利雅得一头骆驼(Camelus dromedarius Linnaeus)中提取的棘头骆驼线粒体基因组。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-02-08 DOI: 10.14411/fp.2022.005
Shona Chandra, Abdullah D Alanazi, Jan Slapeta

Rhipicephalus camicasi Morel, Mouchet et Rodhain, 1976 is thought to be distributed across Africa, Arabian Peninsula and the Mediterranean region. It belongs to the Rhipicephalus sanguineus (Latreille, 1806) species complex. Mitochondrial genome sequences are becoming frequently used for the identification and differentiation of tick species. In the present study, the entire mitochondrial genome of R. cf. camicasi (~15 kb) collected from a camel in Saudi Arabia was sequenced and compared with mitogenomes of two species of Rhipicephalus Koch, 1844. The mitochondrial genome is 87.8% and 91.7% identical to the reference genome of R. sanguineus (sensu stricto, former "temperate lineage") and Rhipicephalus linnaei (Audouin, 1826) (former "tropical lineage"). The current study delivers a molecular reference for material that resembles R. camicasi. We propose to consider the current material, including the complete mitogenome, as the reference for R. camicasi, until a revision using topotypical material is available.

Rhipicephalus camicasi Morel, Mouchet et Rodhain, 1976被认为分布在非洲、阿拉伯半岛和地中海地区。它属于Rhipicephalus sanguineus (Latreille, 1806)种群。线粒体基因组序列被频繁地用于蜱类的鉴定和区分。本研究对沙特阿拉伯骆驼R. cf. camicasi全线粒体基因组(~ 15kb)进行了测序,并与两种Rhipicephalus Koch, 1844的线粒体基因组进行了比较。线粒体基因组与R. sanguineus(严格意义,前“温带谱系”)和Rhipicephalus linnaei (Audouin, 1826)(前“热带谱系”)的参考基因组相同度分别为87.8%和91.7%。目前的研究提供了一种分子参考材料,类似于camicasi。我们建议考虑现有的材料,包括完整的有丝分裂基因组,作为camicasi的参考,直到使用拓扑材料进行修订。
{"title":"Mitochondrial genome of Rhipicephalus cf. camicasi Morel, Mouchet et Rodhain, 1976 from a camel (Camelus dromedarius Linnaeus) in Riyadh, Saudi Arabia.","authors":"Shona Chandra,&nbsp;Abdullah D Alanazi,&nbsp;Jan Slapeta","doi":"10.14411/fp.2022.005","DOIUrl":"https://doi.org/10.14411/fp.2022.005","url":null,"abstract":"<p><p>Rhipicephalus camicasi Morel, Mouchet et Rodhain, 1976 is thought to be distributed across Africa, Arabian Peninsula and the Mediterranean region. It belongs to the Rhipicephalus sanguineus (Latreille, 1806) species complex. Mitochondrial genome sequences are becoming frequently used for the identification and differentiation of tick species. In the present study, the entire mitochondrial genome of R. cf. camicasi (~15 kb) collected from a camel in Saudi Arabia was sequenced and compared with mitogenomes of two species of Rhipicephalus Koch, 1844. The mitochondrial genome is 87.8% and 91.7% identical to the reference genome of R. sanguineus (sensu stricto, former \"temperate lineage\") and Rhipicephalus linnaei (Audouin, 1826) (former \"tropical lineage\"). The current study delivers a molecular reference for material that resembles R. camicasi. We propose to consider the current material, including the complete mitogenome, as the reference for R. camicasi, until a revision using topotypical material is available.</p>","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39821523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Impact of chemoprophylaxis immunisation under halofantrine (CPS-HF) drug cover in Plasmodium yoelii Swiss mice malaria model. 氟茴香碱(CPS-HF)药物覆盖下化学预防免疫对约利疟原虫瑞士小鼠疟疾模型的影响
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-02-03 DOI: 10.14411/fp.2022.003
Arif Jamal Siddiqui, Jyoti Bhardwaj, Walid Sabri Hamadou, Manish Goyal, Sadaf Jahan, Syed Amir Ashraf, Arshad Jamal, Pankaj Sharma, Manojkumar Sachidanandan, Riadh Badraoui, Mejdi Snoussi, Mohd Adnan

In the present study, we have investigated the role of antimalarial drug halofantrine (HF) in inducing the sterile protection against challenges with sporozoites of the live infectious Plasmodium yoelii (Killick-Kendrick, 1967) in Swiss mice malaria model. We observed that during the first to third sequential sporozoite inoculation cycles, blood-stage patency remains the same in the control and chemoprophylaxis under HF drug cover (CPS-HF) groups. However, a delayed blood-stage infection was observed during the fourth and fifth sporozoite challenges and complete sterile protection was produced following the sixth sporozoite challenge in CPS-HF mice. We also noticed a steady decline in liver stage parasite load after 3th to 6th sporozoite challenge cycle in CPS-HF mice. CPS-HF immunisation results in a significant up-regulation of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-12 and iNOS) and down-regulation of anti-inflammatory cytokines (IL-10 and TGF-β) mRNA expression in hepatic mononuclear cells (HMNC) and spleen cells in the immunised CPS-HF mice (after 6th sporozoite challenge) compared to control. Overall, our study suggests that the repetitive sporozoite inoculation under HF drug treatment develops a strong immune response that confers protection against subsequent challenges with sporozoites of P. yoelii.

在本研究中,我们研究了抗疟药氟化茴香碱(HF)在瑞士小鼠疟疾模型中诱导抵抗活感染性约氏疟原虫孢子子攻击的无菌保护作用。我们观察到,在第一个到第三个连续的孢子子接种周期中,在HF药物覆盖(CPS-HF)组的对照和化学预防中,血期通畅保持不变。然而,在CPS-HF小鼠中,在第四和第五次孢子虫攻击期间观察到延迟的血期感染,并且在第六次孢子虫攻击后产生完全的无菌保护。我们还注意到,在CPS-HF小鼠的第3至6个孢子虫攻击周期后,肝脏阶段寄生虫负荷稳步下降。与对照组相比,CPS-HF免疫后,免疫后的小鼠肝单核细胞(HMNC)和脾脏细胞中促炎因子(IFN-γ、TNF-α、IL-12和iNOS)表达显著上调,抗炎因子(IL-10和TGF-β) mRNA表达显著下调。总的来说,我们的研究表明,在HF药物治疗下,反复接种孢子虫可产生强烈的免疫反应,从而保护患者免受约氏假单胞菌孢子虫的侵袭。
{"title":"Impact of chemoprophylaxis immunisation under halofantrine (CPS-HF) drug cover in Plasmodium yoelii Swiss mice malaria model.","authors":"Arif Jamal Siddiqui,&nbsp;Jyoti Bhardwaj,&nbsp;Walid Sabri Hamadou,&nbsp;Manish Goyal,&nbsp;Sadaf Jahan,&nbsp;Syed Amir Ashraf,&nbsp;Arshad Jamal,&nbsp;Pankaj Sharma,&nbsp;Manojkumar Sachidanandan,&nbsp;Riadh Badraoui,&nbsp;Mejdi Snoussi,&nbsp;Mohd Adnan","doi":"10.14411/fp.2022.003","DOIUrl":"https://doi.org/10.14411/fp.2022.003","url":null,"abstract":"<p><p>In the present study, we have investigated the role of antimalarial drug halofantrine (HF) in inducing the sterile protection against challenges with sporozoites of the live infectious Plasmodium yoelii (Killick-Kendrick, 1967) in Swiss mice malaria model. We observed that during the first to third sequential sporozoite inoculation cycles, blood-stage patency remains the same in the control and chemoprophylaxis under HF drug cover (CPS-HF) groups. However, a delayed blood-stage infection was observed during the fourth and fifth sporozoite challenges and complete sterile protection was produced following the sixth sporozoite challenge in CPS-HF mice. We also noticed a steady decline in liver stage parasite load after 3th to 6th sporozoite challenge cycle in CPS-HF mice. CPS-HF immunisation results in a significant up-regulation of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-12 and iNOS) and down-regulation of anti-inflammatory cytokines (IL-10 and TGF-β) mRNA expression in hepatic mononuclear cells (HMNC) and spleen cells in the immunised CPS-HF mice (after 6th sporozoite challenge) compared to control. Overall, our study suggests that the repetitive sporozoite inoculation under HF drug treatment develops a strong immune response that confers protection against subsequent challenges with sporozoites of P. yoelii.</p>","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39612907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
An unexpected tenant: contamination in a Physeter catodon (Physeteridae, Artiodactyla) genome indicates undescribed species of Sarcocystis Lankester, 1882 (Sarcocystidae, Eucoccidiorida) in the marine environment. 一个意想不到的房客:在一个catodon(腕足动物科,偶蹄动物)基因组的污染表明,在海洋环境中,未描述的物种是Lankester, 1882(腕足动物科,Eucoccidiorida)。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-02-03 DOI: 10.14411/fp.2022.004
Joaquim Filipe Faria, David James Harris

Data mining animal of genomes has been used before to identify endoparasites, and may be a particularly useful tool to surpass some difficulties faced by studies in the marine environment. We detected a species of Sarcocystis Lankester, 1882, contamination in the sperm whale (Physeter catodon Linnaeus) reference genome available in the GenBank database. We identified and extracted multiple gene fragments and placed the sequences in a phylogenetic framework. Our results indicate that the sequences of Sarcocystis sp. found in the genome do not correspond to any currently described species, despite a few other similar sequences having been identified in fur seals (Pinnipedia) and another sperm whale. Including data from previous studies, we suggest there is enough evidence to support the occurrence of at least four species of Sarcocystis in marine mammals. We also demonstrate that the term "S. canis-like" has been used for samples not closely related to Sarcocystis canis Dubey et Speer, 1991.

数据挖掘动物基因组之前已被用于鉴定体内寄生虫,并且可能是克服海洋环境研究面临的一些困难的特别有用的工具。我们在GenBank数据库中的抹香鲸(Physeter catodon Linnaeus)参考基因组中检测到一种1882年的Lankester Sarcocystis污染。我们鉴定并提取了多个基因片段,并将序列置于系统发育框架中。我们的研究结果表明,在基因组中发现的Sarcocystis sp.的序列与目前描述的任何物种都不对应,尽管在海狗(Pinnipedia)和另一种抹香鲸中发现了其他一些类似的序列。包括以前的研究数据,我们认为有足够的证据支持海洋哺乳动物中至少有四种肉囊菌的存在。我们还证明,“类犬链球菌”一词已被用于与犬肉囊菌(Sarcocystis canis)不密切相关的样本(Dubey et Speer, 1991)。
{"title":"An unexpected tenant: contamination in a Physeter catodon (Physeteridae, Artiodactyla) genome indicates undescribed species of Sarcocystis Lankester, 1882 (Sarcocystidae, Eucoccidiorida) in the marine environment.","authors":"Joaquim Filipe Faria,&nbsp;David James Harris","doi":"10.14411/fp.2022.004","DOIUrl":"https://doi.org/10.14411/fp.2022.004","url":null,"abstract":"<p><p>Data mining animal of genomes has been used before to identify endoparasites, and may be a particularly useful tool to surpass some difficulties faced by studies in the marine environment. We detected a species of Sarcocystis Lankester, 1882, contamination in the sperm whale (Physeter catodon Linnaeus) reference genome available in the GenBank database. We identified and extracted multiple gene fragments and placed the sequences in a phylogenetic framework. Our results indicate that the sequences of Sarcocystis sp. found in the genome do not correspond to any currently described species, despite a few other similar sequences having been identified in fur seals (Pinnipedia) and another sperm whale. Including data from previous studies, we suggest there is enough evidence to support the occurrence of at least four species of Sarcocystis in marine mammals. We also demonstrate that the term \"S. canis-like\" has been used for samples not closely related to Sarcocystis canis Dubey et Speer, 1991.</p>","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39612906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
New data on the morphology and taxonomy of some spiruridan nematodes (Spirurida) parasitising fishes in Jammu and Kashmir, India. 印度查谟和克什米尔一些寄生鱼类的螺旋体线虫(Spirurida)的形态和分类新资料。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-01-28 DOI: 10.14411/fp.2022.002
Frantisek Moravec, Anshu Chaudhary, Mumtaz Ahmed, Hridaya Shanker Singh
Adult specimens of five little-known species of spiruridan nematodes (Spirurida) were collected during occasional examinations of some fishes from the Poonch River in Jammu and Kashmir, India: Procamallanus (Spirocamallanus) bilaspurensis Gupta et Duggal, 1973 in Mastacembelus armatus (Lacépède) (Mastacembelidae, Synbranchiformes), Rhabdochona (Rhabdochona) indica Moravec, Scholz, Ash et Kar, 2010 in Crossocheilus latius (Hamilton) (Cyprinidae, Cypriniformes), Rhabdochona (Rhabdochona) turkestanica (Skryabin, 1917) in Schizothorax richardsonii (Gray) (Cyprinidae, Cypriniformes) and both Rhabdochona (Rhabdochona) hospeti Thapar, 1950 and Rhabdochona (Globochona) cf. chodukini Osmanov, 1957 in Tor putitora (Hamilton) (Cyprinidae, Cypriniformes); besides these, unidentifiable physalopterid larvae were recorded from M. armatus and T. putitora. Their detailed light and electron microscopical studies revealed some taxonomically important, previously not observed features and made possible their redescription. Fourth-stage larvae of P. (S.) bilaspurensis and R. (R.) turkestanica are described for the first time. The latter is resurrected as an independent species. The following are considered to be species inquirendae: a total of nine poorly described species of Rhabdochona Railliet, 1916 from T. putitora in Balochistan, Pakistan, all listed in the monograph of Kakar et al. (2011), plus an additional 15 species of the genus reported from cyprinids and a catfish in the same region (Balochistan) by the same authors. Also three congeneric species from India (R. nemacheili Rautela et Malhotra, 1982, R. putitora Kaur et Khera, 1991 and R. tori Gupta et Srivastava, 1982) and one from Pakistan (R. charsaddiensis Siddiqi et Khattak, 1984) should be considered as species inquirendae due to their poor descriptions. The names of Rhabdochona putitori Anjum, 2013, R. indusi Soofi, Birmani et Dharejo, 2017 and R. sindhicus Soofi, Birmani, Dharejo, Abbasi et Ghachal, 2020 are invalid according to the International Code of Zoological Nomenclature.
在对印度查谟和克什米尔的Poonch河的一些鱼类进行偶然检查时,收集了五种鲜为人知的螺旋体线虫(Spirurida)的成虫标本:古普塔和杜格尔,1973年,在马齿猴(lacacembelus armatus)(马齿猴科,合branchiformes), Rhabdochona (Rhabdochona) indica Moravec, Scholz, Ash et Kar, 2010年,在Crossocheilus latius (Hamilton)(鲤科,鲤形目),Rhabdochona (Rhabdochona) turkestanica (Skryabin, 1917)在Schizothorax richardsonii (Gray)(鲤科,鲤形目)和Rhabdochona (Rhabdochona) hospeti Thapar, 1950年和Rhabdochona (Globochona) cfchodukini Osmanov,1957年生于托尔普特拉(汉密尔顿)(鲤科,鲤形目);此外,还发现了未被识别的绒纹田鼠和绒纹田鼠的幼虫。他们详细的光学和电子显微镜研究揭示了一些分类学上重要的、以前没有观察到的特征,并使它们的重新描述成为可能。本文首次报道了bilaspurensis和turkestanica的第4期幼虫。后者作为一个独立的物种复活了。以下被认为是调查科的物种:1916年来自巴基斯坦俾路支省T. putitora的Rhabdochona Railliet共有9种描述不清的物种,全部列在Kakar et al.(2011)的专著中,加上同一作者在同一地区(俾路支省)报告的鲤科鱼和鲶鱼的另外15种。此外,来自印度的3个同属种(R. nemacheili Rautela et Malhotra, 1982, R. putitora Kaur et Khera, 1991和R. tori Gupta et Srivastava, 1982)和来自巴基斯坦的1个同属种(R. charsaddiensis Siddiqi et Khattak, 1984)由于描述不佳,应被视为待查种。根据《国际动物命名法》,Rhabdochona putitori Anjum(2013)、R. industrii Soofi, Birmani et Dharejo(2017)和R. sindhicus Soofi, Birmani, Dharejo, Abbasi et Ghachal(2020)的名称无效。
{"title":"New data on the morphology and taxonomy of some spiruridan nematodes (Spirurida) parasitising fishes in Jammu and Kashmir, India.","authors":"Frantisek Moravec,&nbsp;Anshu Chaudhary,&nbsp;Mumtaz Ahmed,&nbsp;Hridaya Shanker Singh","doi":"10.14411/fp.2022.002","DOIUrl":"https://doi.org/10.14411/fp.2022.002","url":null,"abstract":"Adult specimens of five little-known species of spiruridan nematodes (Spirurida) were collected during occasional examinations of some fishes from the Poonch River in Jammu and Kashmir, India: Procamallanus (Spirocamallanus) bilaspurensis Gupta et Duggal, 1973 in Mastacembelus armatus (Lacépède) (Mastacembelidae, Synbranchiformes), Rhabdochona (Rhabdochona) indica Moravec, Scholz, Ash et Kar, 2010 in Crossocheilus latius (Hamilton) (Cyprinidae, Cypriniformes), Rhabdochona (Rhabdochona) turkestanica (Skryabin, 1917) in Schizothorax richardsonii (Gray) (Cyprinidae, Cypriniformes) and both Rhabdochona (Rhabdochona) hospeti Thapar, 1950 and Rhabdochona (Globochona) cf. chodukini Osmanov, 1957 in Tor putitora (Hamilton) (Cyprinidae, Cypriniformes); besides these, unidentifiable physalopterid larvae were recorded from M. armatus and T. putitora. Their detailed light and electron microscopical studies revealed some taxonomically important, previously not observed features and made possible their redescription. Fourth-stage larvae of P. (S.) bilaspurensis and R. (R.) turkestanica are described for the first time. The latter is resurrected as an independent species. The following are considered to be species inquirendae: a total of nine poorly described species of Rhabdochona Railliet, 1916 from T. putitora in Balochistan, Pakistan, all listed in the monograph of Kakar et al. (2011), plus an additional 15 species of the genus reported from cyprinids and a catfish in the same region (Balochistan) by the same authors. Also three congeneric species from India (R. nemacheili Rautela et Malhotra, 1982, R. putitora Kaur et Khera, 1991 and R. tori Gupta et Srivastava, 1982) and one from Pakistan (R. charsaddiensis Siddiqi et Khattak, 1984) should be considered as species inquirendae due to their poor descriptions. The names of Rhabdochona putitori Anjum, 2013, R. indusi Soofi, Birmani et Dharejo, 2017 and R. sindhicus Soofi, Birmani, Dharejo, Abbasi et Ghachal, 2020 are invalid according to the International Code of Zoological Nomenclature.","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39898191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The morphological and molecular description of Neoechinorhynchus (Neoechinorhynchus) poonchensis sp. n. from Schizothorax richardsonii (Gray) in Poonch, Jammu and Kashmir, India. 印度查谟和克什米尔邦Poonch地区Schizothorax richardsonii (Gray)的Neoechinorhynchus (Neoechinorhynchus) poonchensis sp. n.的形态和分子描述。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2022-01-05 DOI: 10.14411/fp.2022.001
Omar Mohamed Amin, Mumtaz Ahmed, Anshu Chaudhary, Richard Anderson Heckmann, Hridaya Shanker Singh

Specimens of Neoechinorhynchus (Neoechinorhynchus) poonchensis sp. n. are described from Schizothorax richardsonii (Gray) in the Poonch River, Jammu and Kashmir. Specimens are thick-walled with dissimilar dorsal and ventral para-receptacle structures, anteriorly manubriated hooks, two giant nuclei in each lemniscus and many subcutaneousy. The lemnisci barely overlap the larger anterior testis, the cement gland has eight giant nuclei, and the seminal vesicle is large with thin walls. The vagina is unremarkable but the long uterus is made up of four specialised regions. Neoechinorhynchus rigidus (Van Cleave, 1928), resembles N. poonchensis sp. n. It is distinguished from N. poonchensis sp. n. by having smaller trunk, proboscis, and male reproductive structures, equal testes, unequal lemnisci with three giant nuclei each, and much larger anterior proboscis hook (130 μm in males) than that originally described by Van Cleave (1928) (70 μm in a female). Anterior hook length alone is sufficient to conclude that the N. rigidus of Datta (1937) is not the same species as the N. rigidus of Van Cleave (1928). Van Cleave's (1928) species remains valid and that of Datta (1937) is considered a different species named Neoechinorhynchus pseudorigidus sp. n., herein. Micropores of N. poonchensis sp. n. have variable distribution in different trunk regions and the Energy Dispersive X-ray analysis demonstrated higher levels of sulfur and lower levels of calcium and phosphorus. Sequences of the 18S rDNA gene from nuclear DNA, and cytochrome c oxidase subunit I (cox1) from mitochondrial DNA of N. poonchensis sp. n. were amplified and aligned with other sequences available on GenBank. Maximum likelihood (ML) and Bayesian inference (BI) analyses inferred for 18S rDNA and cox1 showed that N. poonchensis sp. n. was nested in a separate clade.

在查谟和克什米尔的Poonch河中描述了新echinorhynchus (Neoechinorhynchus) poonchensis sp. n.的标本。标本壁厚,具有不同的背侧和腹侧托旁结构,前侧有钩,每个小网膜内有两个巨大核,皮下有许多。肾盂仅与较大的前睾丸重叠,水泥腺有8个巨大核,精囊大而壁薄。阴道并不引人注目,但长长的子宫由四个专门的区域组成。neechinorhynchus rigidus (Van Cleave, 1928),与n. poonchensis sp. n.相似。它与n. poonchensis sp. n.的区别在于,它有更小的躯干、长鼻和雄性生殖结构,相等的睾丸,不等长的喙,每个都有三个巨大的核,以及比Van Cleave(1928)最初描述的大得多的前喙钩(雄性130 μm)(雌性70 μm)。单凭前钩长度就足以断定Datta(1937)的N. rigidus与Van Cleave(1928)的N. rigidus不是同一种。Van Cleave(1928)的物种仍然有效,Datta(1937)的物种被认为是一个不同的物种,命名为neechinorhynchus pseudorigidus sp. n.。不同树干区域的微孔分布不同,能量色散x射线分析表明,微孔中硫含量较高,钙、磷含量较低。扩增了N. poonchensis sp. N.核DNA中的18S rDNA基因序列和线粒体DNA中的细胞色素c氧化酶亚基I (cox1)序列,并与GenBank上的其他序列比对。对18S rDNA和cox1的最大似然(ML)和贝叶斯推断(BI)分析表明,N. poonchensis sp. N.嵌套在一个单独的分支中。
{"title":"The morphological and molecular description of Neoechinorhynchus (Neoechinorhynchus) poonchensis sp. n. from Schizothorax richardsonii (Gray) in Poonch, Jammu and Kashmir, India.","authors":"Omar Mohamed Amin,&nbsp;Mumtaz Ahmed,&nbsp;Anshu Chaudhary,&nbsp;Richard Anderson Heckmann,&nbsp;Hridaya Shanker Singh","doi":"10.14411/fp.2022.001","DOIUrl":"https://doi.org/10.14411/fp.2022.001","url":null,"abstract":"<p><p>Specimens of Neoechinorhynchus (Neoechinorhynchus) poonchensis sp. n. are described from Schizothorax richardsonii (Gray) in the Poonch River, Jammu and Kashmir. Specimens are thick-walled with dissimilar dorsal and ventral para-receptacle structures, anteriorly manubriated hooks, two giant nuclei in each lemniscus and many subcutaneousy. The lemnisci barely overlap the larger anterior testis, the cement gland has eight giant nuclei, and the seminal vesicle is large with thin walls. The vagina is unremarkable but the long uterus is made up of four specialised regions. Neoechinorhynchus rigidus (Van Cleave, 1928), resembles N. poonchensis sp. n. It is distinguished from N. poonchensis sp. n. by having smaller trunk, proboscis, and male reproductive structures, equal testes, unequal lemnisci with three giant nuclei each, and much larger anterior proboscis hook (130 μm in males) than that originally described by Van Cleave (1928) (70 μm in a female). Anterior hook length alone is sufficient to conclude that the N. rigidus of Datta (1937) is not the same species as the N. rigidus of Van Cleave (1928). Van Cleave's (1928) species remains valid and that of Datta (1937) is considered a different species named Neoechinorhynchus pseudorigidus sp. n., herein. Micropores of N. poonchensis sp. n. have variable distribution in different trunk regions and the Energy Dispersive X-ray analysis demonstrated higher levels of sulfur and lower levels of calcium and phosphorus. Sequences of the 18S rDNA gene from nuclear DNA, and cytochrome c oxidase subunit I (cox1) from mitochondrial DNA of N. poonchensis sp. n. were amplified and aligned with other sequences available on GenBank. Maximum likelihood (ML) and Bayesian inference (BI) analyses inferred for 18S rDNA and cox1 showed that N. poonchensis sp. n. was nested in a separate clade.</p>","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39874800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Detection and molecular characterisation of intestinal parasites in the South China tiger Panthera tigris amoyensis (Hilzheimer). 华南虎(Panthera tigris amoyensis, Hilzheimer)肠道寄生虫的检测与分子特征分析。
IF 1.6 4区 医学 Q2 Medicine Pub Date : 2021-12-31 DOI: 10.14411/fp.2021.029
Hung-Chuan Chiu, Kewei Fan, Xiaoshuang Sun, Kaixiong Lin, Tengteng Chen, Fei Yang, Yunfei Qiu, Dongxia Wei, Cuiqin Huang

Parasitic infections of the South China tigers in the Meihua Mountains have not been explored previously. Faeces of 22 South China tigers from the China Tiger Park in the Meihua Mountains were examined. Eggs of ascaridoid nematodes and oocysts of coccidia were detected by Mini-FLOTAC assay. Morphological observation and molecular characterisation of the oocysts were carried out. The prevalence of Toxascaris leonina (von Linstow, 1902) was 18% (4/22), and the highest egg per gram (EPG) count in the faeces was 27,150. The prevalence of Cystoisospora sp. was 45% (1 0/22) and the highest oocysts per gram (OPG) in the faeces was 6,000. In addition, we found one ascaridoid nematode in the South China tiger's faeces and was molecularly and morphologically identified as T. leonina. The oocysts in the faeces were sporulated in vitro and identified as Cystoisospora sp. Amplification of full-length internal transcribed spacers (ITS) resulted in sequences 1,622 bp long. Using the sequences, Cystoisospora sp. of the South China tiger was closest to Isospora belli (Wenyon, 1923) and Cystoisospora suis (Biester, 1934).

梅花山华南虎寄生虫感染的研究尚未见报道。对梅花山中国虎园22只华南虎的粪便进行了检测。采用Mini-FLOTAC法对蛔虫卵和球虫卵囊进行检测。对卵囊进行了形态学观察和分子特征分析。里昂弓形虫(von Linstow, 1902)的流行率为18%(4/22),粪便中最高每克卵数为27,150个。囊异孢子虫感染率为45%(1 /22),粪便中最高卵囊数为6000个/克。此外,我们还在华南虎粪便中发现了一种蛔虫,经分子和形态学鉴定为T. leonina。对粪卵囊进行体外产孢,鉴定为Cystoisospora sp.全长内转录间隔段(ITS)扩增,序列长1,622 bp。利用该序列,华南虎的囊异孢子虫属与belli Isospora (Wenyon, 1923)和suis Cystoisospora (Biester, 1934)最接近。
{"title":"Detection and molecular characterisation of intestinal parasites in the South China tiger Panthera tigris amoyensis (Hilzheimer).","authors":"Hung-Chuan Chiu,&nbsp;Kewei Fan,&nbsp;Xiaoshuang Sun,&nbsp;Kaixiong Lin,&nbsp;Tengteng Chen,&nbsp;Fei Yang,&nbsp;Yunfei Qiu,&nbsp;Dongxia Wei,&nbsp;Cuiqin Huang","doi":"10.14411/fp.2021.029","DOIUrl":"https://doi.org/10.14411/fp.2021.029","url":null,"abstract":"<p><p>Parasitic infections of the South China tigers in the Meihua Mountains have not been explored previously. Faeces of 22 South China tigers from the China Tiger Park in the Meihua Mountains were examined. Eggs of ascaridoid nematodes and oocysts of coccidia were detected by Mini-FLOTAC assay. Morphological observation and molecular characterisation of the oocysts were carried out. The prevalence of Toxascaris leonina (von Linstow, 1902) was 18% (4/22), and the highest egg per gram (EPG) count in the faeces was 27,150. The prevalence of Cystoisospora sp. was 45% (1 0/22) and the highest oocysts per gram (OPG) in the faeces was 6,000. In addition, we found one ascaridoid nematode in the South China tiger's faeces and was molecularly and morphologically identified as T. leonina. The oocysts in the faeces were sporulated in vitro and identified as Cystoisospora sp. Amplification of full-length internal transcribed spacers (ITS) resulted in sequences 1,622 bp long. Using the sequences, Cystoisospora sp. of the South China tiger was closest to Isospora belli (Wenyon, 1923) and Cystoisospora suis (Biester, 1934).</p>","PeriodicalId":55154,"journal":{"name":"Folia Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
期刊
Folia Parasitologica
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1