Cytopathology最新文献_第10页

Clinical Performance of a Liquid Preservation Medium for Cervicovaginal Samples in DNA-HPV Testing and Liquid-Based Cytology for Cervical Cancer Screening 宫颈阴道样本液体保存介质在DNA-HPV检测和宫颈癌筛查中的临床表现。

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-23 DOI: 10.1111/cyt.13495

Larissa Dias Assunção, Michelle Garcia Discacciati, Andressa Germano da Silva, Adriana Yoshida, Diama Bhadra Vale, Julio Cesar Teixeira

Introduction

Cervical cancer remains a significant global health concern, primarily associated with persistent infections by high-risk human papillomavirus (hr-HPV). As screening programmes evolve from traditional cytology to DNA-HPV testing, the need for a liquid medium that maintains the integrity of cervical samples for biomolecular analysis and cytology becomes critical.

Methods

This study evaluated the performance of the candidate liquid preservation medium (PM) Cytoliq for cervical samples intended for DNA-HPV testing and liquid-based cytology (LBC), in comparison with the reference PM, PreservCyt-ThinPrep. A total of 112 women aged 18–64 years underwent routine gynaecological examinations, with paired cervical samples preserved in both PM for HPV testing and genotyping (Cobas HPV test), and LBC. The study aimed for a sensitivity greater than 90% in detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2+), moderate to high agreement in HPV testing results (Kappa index > 0.70) and adequate performance in LBC.

Results

The candidate PM exhibited non-inferior performance relative to the reference PM. DNA-HPV testing showed a 94.5% agreement rate (Kappa = 0.88) and a sensitivity of 92.9% for CIN2+ detection. Additionally, the candidate PM performed well in LBC smear production, with no significant differences in cytological diagnoses. The agreement in LBC diagnoses was 94.0% (Kappa = 0.79) with the ThinPrep processor and 91.8% (Kappa = 0.63) with the Cytoliq processor.

Conclusion

The Cytoliq PM demonstrated comparable efficacy to the reference for DNA-HPV testing and LBC, supporting its potential as an alternative preservation medium in cervical cancer screening programmes.

宫颈癌仍然是一个重要的全球健康问题，主要与高危人乳头瘤病毒（hr-HPV）的持续感染有关。随着筛查计划从传统细胞学发展到DNA-HPV检测，需要一种液体培养基来保持宫颈样本的完整性，以进行生物分子分析和细胞学分析变得至关重要。方法：本研究评估了用于DNA-HPV检测和液体细胞学（LBC）的宫颈样本的候选液体保存介质（PM） Cytoliq的性能，并与参考PM PreservCyt-ThinPrep进行比较。共有112名年龄在18-64岁的女性接受了常规妇科检查，并在PM中保存了配对的宫颈样本，用于HPV检测和基因分型（Cobas HPV检测）和LBC。该研究旨在检测宫颈上皮内瘤变2级或更糟（CIN2+）的灵敏度大于90%，HPV检测结果（Kappa指数> 0.70）的一致性中等至高度，并在LBC中有足够的表现。结果：候选PM的表现不逊于参考PM。DNA-HPV检测的符合率为94.5% (Kappa = 0.88)， CIN2+检测的敏感性为92.9%。此外，候选PM在LBC涂片生产中表现良好，细胞学诊断无显着差异。ThinPrep处理器诊断LBC的一致性为94.0% (Kappa = 0.79)， Cytoliq处理器诊断LBC的一致性为91.8% （Kappa = 0.63）。结论：Cytoliq PM在DNA-HPV检测和LBC检测中表现出与参比相当的功效，支持其作为宫颈癌筛查计划的替代保存介质的潜力。

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引用次数: 0

Cytomorphological and Immunohistochemical Features of Metastatic Malignant Phyllodes Tumour in Pleural Fluid: A Case Report With Literature Review 胸腔液中转移性叶状瘤的细胞形态学和免疫组织化学特征：1例报告并文献复习。

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-21 DOI: 10.1111/cyt.13501

Yi Xu, Xiao-Yu Nie, Qian Wang, Zhi-Hong Xian, Shu-Hui Zhang

The cytomorphology of malignant phyllodes tumour (MPT) in effusion specimens is rarely described. In this paper, the detailed cytopathological and immunohistochemical characteristics of metastatic MPT have been described in pleural effusion. Patients' medical history and the judicious utilisation of ancillary studies including cellblock and immunocytochemistry contribute to ensure precise cytological diagnoses.

The cytomorphology of malignant phyllodes tumour (MPT) in effusion specimens can be diagnostically challenging. The author presents detailed cytopathological and immunohistochemical characteristics of a case of metastatic MPT in pleural effusion.

恶性叶状瘤（MPT）的细胞形态学在积液标本中很少被描述。本文详细描述了转移性MPT在胸腔积液中的细胞病理学和免疫组织化学特征。患者的病史和辅助研究的明智利用，包括细胞阻滞和免疫细胞化学有助于确保精确的细胞学诊断。恶性叶状瘤（MPT）在积液标本中的细胞形态学诊断具有挑战性。本文报告一例胸腔积液转移性MPT的细胞病理学和免疫组织化学特征。

引用次数: 0

Villoglandular Carcinoma of the Uterine Cervix: Clinic-Cytopathological-Histological Features of a Rare Case With Brief Review of the Literature 子宫颈绒毛腺癌：一例罕见病例的临床-细胞病理-组织学特征并简要复习文献。

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-21 DOI: 10.1111/cyt.13492

Neelam Sood, Ruchika Gupta, Sanjay Gupta

Villoglandular adenocarcinoma (VGA) of the cervix is a rare tumour with very few reports of cytological diagnosis on a cervical smear. Since the usual prognosis of VGA is more favourable than that of a conventional cervical adenocarcinoma, a pre-operative diagnosis is essential for appropriate therapeutic decisions. We report the clinical, cytological, and histopathological features of a case of VGA in an elderly woman. A 60-year-old female presented with postmenopausal bleeding and was found to have a hypertrophied cervix. Conventional smears from the cervix demonstrated features of atypical glandular cells with a villoglandular configuration. Endocervical cell nuclei were enlarged with overlapping, hyperchromasia and mild to moderate anisonucleosis. The cytological impression of villoglandular carcinoma was confirmed on subsequent hysterectomy and histopathology. Hence, cytopathologists need to be aware of the entity of villoglandular carcinoma of the cervix, its relatively bland nuclear features, and the diagnostic clues to allow for an accurate diagnosis on cervical smear. An accurate pre-operative diagnosis can assist the gynaecologists in arriving at appropriate therapeutic and prognostic decisions.

子宫颈绒毛腺腺癌（VGA）是一种罕见的肿瘤，很少报告细胞学诊断宫颈涂片。由于VGA的预后通常比传统的子宫颈腺癌更有利，因此术前诊断对于适当的治疗决策至关重要。我们报告一位老年妇女的VGA病例的临床、细胞学和组织病理学特征。一个60岁的女性提出绝经后出血和发现有一个肥大的宫颈。子宫颈常规涂片显示非典型腺细胞的特征，具有绒毛腺状结构。宫颈内细胞核增大，有重叠、色素增多和轻至中度核异核增多。随后的子宫切除术和组织病理学证实了绒毛腺癌的细胞学印象。因此，细胞病理学家需要了解子宫颈腺绒毛癌的实质，其相对平淡的核特征，以及诊断线索，以便在子宫颈细胞涂片上准确诊断。准确的术前诊断可以帮助妇科医生做出适当的治疗和预后决定。

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引用次数: 0

Primary Hepatoid Adenocarcinoma of the Lung: Clues for Cytological Diagnosis 原发性肺肝样腺癌：细胞学诊断的线索。

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-14 DOI: 10.1111/cyt.13494

M. J. Fernández Aceñero, V. Acedo, P. Barreiro, M. Negueruela, L. Nielsen

Hepatoid carcinoma is an aggressive and infrequent type of tumour. Lung is a very infrequent location of this tumour. Hepatoid carcinoma can be suspected from cytological features. Immunocytochemistry can confirm suspicion in cytological samples.

肝样腺癌早在20世纪80年代就被发现，但直到20世纪90年代才有明确的诊断标准。诊断最初是基于与肿瘤相关的血清甲胎蛋白升高，但随着时间的推移，一些具有肝样腺癌形态学和免疫组织化学特征的病变与甲胎蛋白升高无关，不再需要诊断。最近的系统综述描述了不到70例原发性肺肝样腺癌，其中大多数病例是通过活检诊断的。病例介绍：我们在此报告一位67岁男性患者，通过细胞学检查诊断为原发性肺肝样腺癌，强调细胞学特征应该导致怀疑这种诊断。结论：很少有报道描述了这种肿瘤的细胞学特征，但重要的是要定义和认识它们，因为细胞学在许多情况下是诊断和治疗肺肿瘤患者的唯一可用样本。此外，肝样腺癌似乎对常规治疗有耐药性，必须认识到它，避免与其他类型的肿瘤混淆。

引用次数: 0

Liquid Biopsy in Solid Tumours: An Overview 固体肿瘤液体活检：综述。

IF 1.2 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-11 DOI: 10.1111/cyt.13485

Pasquale Pisapia, Antonino Iaccarino, Giancarlo Troncone, Umberto Malapelle

The advent of personalised and precision medicine has radically modified the management and the clinical outcome of cancer patients. However, the expanding number of predictive, prognostic, and diagnostic biomarkers has raised the need for simple, noninvasive, quicker, but equally efficient tests for molecular profiling. In this complex scenario, the adoption of liquid biopsy, particularly circulating tumour DNA (ctDNA), has been a real godsend for many cancer patients who would otherwise have been denied the benefits of targeted treatments. Undeniably, ctDNA analysis has several advantages over conventional tissue-based analysis. One advantage is that it can guide treatment decision making, especially when tissue samples are scarce or totally unavailable. Indeed, a simple blood test can inform clinicians on patients' response or resistance to targeted therapies, help them monitor minimal residual disease (MRD) after surgical resections, and facilitate them with early cancer detection and interception. Finally, an equally important advantage is that ctDNA analysis can help decipher temporal and spatial tumour heterogeneity, a mechanism highly responsible for therapeutic resistance. In this review, we gathered and analysed current evidence on the clinical usefulness of ctDNA analysis in solid tumours.

个性化和精准医疗的出现从根本上改变了癌症患者的管理和临床结果。然而，随着预测性、预后性和诊断性生物标志物数量的不断增加，对简单、无创、快速、但同样有效的分子谱分析测试的需求日益增加。在这种复杂的情况下，采用液体活检，特别是循环肿瘤DNA (ctDNA)，对许多癌症患者来说是一个真正的天赐之物，否则他们将被剥夺靶向治疗的好处。不可否认，ctDNA分析比传统的基于组织的分析有几个优势。一个优点是它可以指导治疗决策，特别是当组织样本稀缺或完全无法获得时。事实上，一项简单的血液检查可以让临床医生了解患者对靶向治疗的反应或耐药性，帮助他们监测手术切除后的最小残留疾病（MRD），并促进他们进行早期癌症检测和拦截。最后，一个同样重要的优势是，ctDNA分析可以帮助破译肿瘤的时间和空间异质性，这是一种高度负责治疗耐药性的机制。在这篇综述中，我们收集并分析了目前关于ctDNA分析在实体肿瘤中的临床应用的证据。

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引用次数: 0

Investigating Career Advancement in Academic Cytopathology Workforce: A Gender and Regional Comparison 研究学术细胞病理学工作人员的职业发展：性别和地区比较。

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-10 DOI: 10.1111/cyt.13490

Paul Z. Chiou, Yuane Jia, Rema Vinod

Objective

Using bibliometric analysis to inform factors impacting career mobility, focusing on gender and regional variations in academic cytopathology.

Methods

The Scopus database was used to determine the productivity and experience of cytopathologists who are faculty from the 92 fellowship programs. The dependent variable was promotion to the rank of Professor. A regression analysis was conducted with productivity (h-index), experience (years in publication) and one's geographic work location as covariates to investigate the role of gender in promotion.

Results

The gender distribution is skewed towards females at the Assistant (62.4% vs. 37.58%) and Associate Professors (70.51% vs. 29.49%) levels, but the gap narrows at the full-professor rank (52.98% vs. 47.02%). The regression model showed significant regional variation in promotion for the Southern (0.35; 95% CI, 0.16–0.79; p = 0.012) and Northeastern (0.24; 95% CI, 0.10–0.57; p = 0.001) regions compared to the West. Active years of publishing (1.18; 95% CI, 1.11–1.27; p < 0.001) and productivity (1.09; 95% CI, 1.06–1.12; p < 0.001) were factors in promotion. The mean HI productivity was comparable between the males and females in the first (4.41 for males and 4.40 for females) and the second decade (13.81 for males and 12.84 for females). However, the gap widened into the third decade (30.27 for males and 26.40 for females), suggesting potential areas for improvement.

Conclusion

While the academic cytopathology workforce is progressing towards gender equity, there is still room for improvement. Our findings suggest that focusing on mid-career professionals(between the second to third decades) by providing additional resources for research and facilitating work–life flexibility can help close the gender gap at the highest level.

目的：利用文献计量学分析了解影响职业流动性的因素，重点关注学术细胞病理学的性别和地区差异。方法：使用Scopus数据库来确定来自92个奖学金项目的细胞病理学家的生产力和经验。因变量是晋升到教授级别。以生产力（h-index）、经验（出版年数）和地理工作地点为协变量进行回归分析，探讨性别在晋升中的作用。结果：在助教（62.4% vs. 37.58%）和副教授（70.51% vs. 29.49%）级别，性别分布向女性倾斜，但在正教授级别（52.98% vs. 47.02%），性别差距缩小。回归模型显示，南方地区的晋升率存在显著的区域差异(0.35；95% ci, 0.16-0.79；p = 0.012)和东北(0.24；95% ci, 0.10-0.57；p = 0.001)。出版活跃年数(1.18；95% ci, 1.11-1.27；p结论：虽然学术细胞病理学工作人员正在朝着性别平等的方向发展，但仍有改进的空间。我们的研究结果表明，通过提供额外的研究资源和促进工作与生活的灵活性，关注职业生涯中期的专业人士（在第二到第三个十年之间），可以帮助在最高水平上缩小性别差距。

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引用次数: 0

Performance of the Monoclonal Antibody B72.3 in Diagnosis of Malignant Carcinomatous Serous Effusions—A Systematic Review and Meta-Analysis of Diagnostic Performance 单克隆抗体B72.3在恶性肿瘤浆液积液诊断中的作用——诊断效果的系统评价和荟萃分析

IF 1.2 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-10 DOI: 10.1111/cyt.13493

Alex H. Lin, Matthew Hsu, Joanna K. M. Ng, Sahar J. Farahani, Joshua J. X. Li, Jana Nano, Hamidreza Raeisi-Dehkordi, Wilson Tang, Philippe Vielh, Taulant Muka

Objectives

Immunocytochemistry is often required in the cytologic assessment of malignant serous effusion, particularly for differentiating metastatic carcinoma from mesothelioma. To summarise the diagnostic performance of the monoclonal antibody B72.3, a systematic review and meta-analysis was conducted.

Methods

Five databases were searched for relevant studies and reviewed for data extraction and risk of bias assessment. Pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under curve of summary receiver operating characteristics (AUC-SROC) were calculated for the diagnostic performance of B72.3. Heterogeneity and publication bias were assessed by the I² index and Deeks' funnel plot.

Results

In total, 19 studies (1159 cases) were included. Overall pooled sensitivity and specificity were 0.76 (0.72–0.79) and 0.90 (0.74–1.00), respectively. The NLR, PLR and DOR were 0.27 (0.21–0.34), 7.66 (< 0.001–20.46) and 28.26 (0–75.96), respectively. The AUC-SROC was 0.98, indicating a good overall diagnostic accuracy for B72.3. Subgroup analysis for adenocarcinoma (0.75, 0.71–0.79), mesothelioma (0.92, 0.85–0.98) and benign/reactive mesothelial cells (0.96, 0.93–1.00) showed similar sensitivity and specificity, while the sensitivity for adenocarcinomas of the gastrointestinal/hepatobiliary tract (0.56, 0.41–0.71) and breast (0.55, 0.38–0.71) was significantly lower. High heterogeneity was observed in the majority of our analyses, while no evidence of publication bias was identified.

Conclusions

B72.3 has an acceptable performance with low sensitivity. With a good specificity, B72.3 may find use in an immunocytochemical panel for excluding benign mesothelial processes.

目的：在恶性浆液积液的细胞学评估中经常需要免疫细胞化学，特别是在鉴别转移癌和间皮瘤时。为了总结单克隆抗体B72.3的诊断性能，我们进行了系统评价和荟萃分析。方法：检索5个数据库的相关研究，进行资料提取和偏倚风险评估。计算B72.3诊断效能的综合敏感性、特异性、阳性似然比（PLR）、阴性似然比（NLR）、诊断优势比（DOR）和综合受试者工作特征曲线下面积（AUC-SROC）。采用I2指数和Deeks漏斗图评估异质性和发表偏倚。结果：共纳入19项研究（1159例）。总体合并敏感性和特异性分别为0.76（0.72-0.79）和0.90（0.74-1.00）。NLR、PLR和DOR分别为0.27（0.21-0.34）、7.66(结论：B72.3在低灵敏度下性能尚可。B72.3具有良好的特异性，可用于免疫细胞化学检查，以排除良性间皮过程。

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引用次数: 0

Evaluation of Diagnostic Accuracy of Directly Sampled Endometrial Cytology Using ThinPrep for Endometrial Malignancies: Comparison With Existing Endometrial Liquid-Based Cytology 评估使用ThinPrep直接取样子宫内膜细胞学诊断子宫内膜恶性肿瘤的准确性：与现有子宫内膜液基细胞学的比较。

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-07 DOI: 10.1111/cyt.13488

Rie Ikemoto, Ken Tanikawa, Morio Ijichi, Miyoko Miyakawa, Kenji Yanoh, Jun Watanabe, Yutaka Nakamura, Ken Maruyama, Tadao K. Kobayashi, Hideaki Hirai, Noriyuki Furuta, Yasuo Hirai

Objective

The aim of this study was to evaluate the accuracy of detecting malignancies by directly sampled endometrial cytology using globally adopted ThinPrep with a novel preparation technique.

Methods

Medical records and reports of pathology and cytology from June 2019 to March 2022 were reviewed. We selected 112 endometrial cytology specimens using ThinPrep with the novel preparation technique, where the clinical course or pathological samples confirmed negative or positive results. Eight cytotechnologists or cytopathologists examined the cytology specimens and provided reports based on standardised criteria from the descriptive reporting system for endometrial cytology (the Yokohama System).

Results

The 112 specimens were evenly smeared and well prepared, featuring high quality, with no issues hindering microscopic examination. Examiners unfamiliar with endometrial cytology using ThinPrep showed high diagnostic accuracy, demonstrating that this modality of preparation for endometrial cytology is feasible for clinical use.

Conclusions

The novel preparation method using ThinPrep successfully provided high-quality, standardised specimens. Furthermore, employing the Yokohama System enabled high accuracy in detecting endometrial malignancies, even for examiners with minimal experience with this cytological technique. This suggests that ThinPrep-based endometrial cytology can be globally adopted with ease, potentially contributing significantly to the early detection of endometrial cancer.

目的：本研究的目的是评估使用全球通用的新型制备技术ThinPrep直接取样子宫内膜细胞学检测恶性肿瘤的准确性。方法：回顾2019年6月至2022年3月的病历及病理、细胞学报告。我们选择了112例子宫内膜细胞学标本，采用新型制备技术，临床过程或病理样本证实阴性或阳性。8名细胞技术专家或细胞病理学家检查细胞学标本，并根据子宫内膜细胞学描述性报告系统（横滨系统）的标准化标准提供报告。结果：112例标本涂片均匀，制备充分，质量高，无妨碍显微镜检查的问题。不熟悉子宫内膜细胞学的检查人员使用ThinPrep显示出较高的诊断准确性，表明这种子宫内膜细胞学准备方式在临床应用是可行的。结论：ThinPrep制备方法成功地提供了高质量、标准化的标本。此外，采用横滨系统可以高精度地检测子宫内膜恶性肿瘤，即使对于没有细胞学技术经验的检查人员也是如此。这表明，基于thinprep的子宫内膜细胞学可以在全球范围内轻松采用，可能对子宫内膜癌的早期发现做出重大贡献。

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引用次数: 0

The Diagnostic Role of TFF1, TFF3, FOXA1, CA XII and TRPS1 in Serous Effusions TFF1、TFF3、FOXA1、caxii和TRPS1在浆液积液中的诊断作用

IF 1.2 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-01 DOI: 10.1111/cyt.13489

Ben Davidson, Arild Holth, Jeremias Wohlschlaeger, Maurizio Pinamonti, Binnur Önal

Objective

To analyse the diagnostic role of trefoil factor-1 and -3 (TFF1, TFF3), forkhead box protein A1 (FOXA1), carbonic anhydrase XII (CA XII) and trichorhinophalangeal syndrome type 1 (TRPS1) in serous effusions. The prognostic role of these markers in breast carcinoma was additionally studied.

Methods

Protein expression by immunohistochemistry was analysed in 247 effusions, consisting of 60 breast carcinomas, 54 tubo-ovarian carcinomas, 47 mesotheliomas, 44 lung carcinomas, 20 uterine corpus and cervical carcinomas, 17 gastrointestinal carcinomas and 5 cancers of other origin.

Results

TFF1, TFF3, FOXA1, CA XII and TRPS1 expression was found in 67%, 70%, 88%, 82% and 83% of breast carcinomas, respectively. Expression of all markers was seen in some carcinomas of other origin, most commonly in GI metastases, but was least frequent for TRPS1. CA XII expression was additionally seen in mesotheliomas and reactive mesothelial cells. All 5 markers were significantly overexpressed in breast compared to tubo-ovarian carcinoma (all p < 0.001) and lung carcinoma (all p < 0.001 except for FOXA1, p = 0.023). TFF1 (p = 0.003), TFF3 (p = 0.008) and FOXA1 (p = 0.017) expression was significantly higher in receptor-positive compared to receptor-negative primary breast carcinomas. In survival analysis for 44 breast carcinoma patients with clinical data, TFF1 expression was associated with a trend for longer overall (p = 0.096) and disease-free (p = 0.06) survival.

Conclusion

TFF1, TFF3, FOXA1, CA XII and TRPS1 are sensitive breast carcinoma markers, with FOXA1 performing best in terms of sensitivity and TRPS1 being the most specific. Whether the expression of these markers in breast carcinoma effusions is informative of survival merits further research.

目的分析三叶因子-1和-3（TFF1和TFF3）、叉头盒蛋白A1（FOXA1）、碳酸酐酶XII（CA XII）和毛细血管畸形综合征1型（TRPS1）在浆液性渗出液中的诊断作用。此外，还研究了这些标记物在乳腺癌中的预后作用：方法：对 247 例渗出液进行免疫组织化学分析，其中包括 60 例乳腺癌、54 例输卵管卵巢癌、47 例间皮瘤、44 例肺癌、20 例子宫体癌和宫颈癌、17 例胃肠道癌和 5 例其他来源的癌症：结果发现，67%、70%、88%、82%和83%的乳腺癌中分别有TFF1、TFF3、FOXA1、CA XII和TRPS1的表达。所有标记物的表达均见于一些其他来源的癌症，最常见于消化道转移癌，但TRPS1的表达最少。CA XII还可见于间皮瘤和反应性间皮细胞。与输卵管卵巢癌相比，所有 5 个标记物在乳腺癌中均明显过表达（均为 p 结论：TFF1、TFF3、TFF4、TFF5、TFF6、TFF7、TFF8、TFF9、TFF10、TFF11、TFF12）：TFF1、TFF3、FOXA1、CA XII 和 TRPS1 是敏感的乳腺癌标记物，其中 FOXA1 的敏感性最好，TRPS1 的特异性最高。这些标记物在乳腺癌渗出液中的表达是否能反映患者的生存情况，值得进一步研究。

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引用次数: 0

Impact of Cold Ischemic Time on Nucleic Acid Quality in Cytology Samples for Cancer Gene Analysis 冷缺血时间对肿瘤基因分析细胞学样品核酸质量的影响。

IF 1.2 4区医学 Q4 CELL BIOLOGY

Cytopathology

Pub Date : 2025-04-01 DOI: 10.1111/cyt.13491

Hideyuki Abe, Akihiko Kawahara, Ryou Makino, Takato Kumagae, Jun Akiba, Hironori Kusano

Objective

Despite the growing attention on the use of cytology samples for genetic analysis, the impact of the time from sample collection to fixation, referred to as ‘cold’ ischemic time, has not been sufficiently studied. Therefore, we investigated the quality of nucleic acids prior to fixation in body cavity fluid samples, focusing on the benign/malignant status, differences in collection methods and tumour cell content.

Methods

We analysed 49 body cavity fluid samples collected using different methods: aspiration (n = 21), drainage (n = 5), and surgery (n = 15). The samples were collected from 26 malignant and 23 benign cases. DNA and RNA were extracted from all samples, and their yield and quality were assessed using Agilent TapeStation. Tumour cell content was calculated as the ratio of malignant to benign cells.

Results

DNA and RNA yields were significantly higher in malignant than in benign cases (p < 0.05). Regarding nucleic acid quality, there was a significant difference in RNA quality between malignant and benign cases, but no significant difference in DNA quality. Among the 26 malignant cases, there were significant differences in integrity number (RIN) and RNA percentage of nucleic acid fragments with > 200 nucleotides (DV200) between samples collected via aspiration and surgery (p < 0.05). Tumour cell content (median, 36%; range, 20%–72%) showed no correlation with nucleic acid yield or quality.

Conclusions

This study's findings suggest that DNA extracted from cytology samples is highly stable, while RNA is affected by cold ischemic time. Thus, prompt fixation after collection is necessary to maintain RNA quality.

目的：尽管人们越来越关注使用细胞学样本进行遗传分析，但从样本收集到固定的时间（称为“冷”缺血时间）的影响尚未得到充分研究。因此，我们对体腔液标本固定前的核酸质量进行了调查，重点关注其良/恶性状态、采集方法的差异和肿瘤细胞含量。方法：对49份体腔液标本进行分析，分别采用抽吸（n = 21）、引流（n = 5）和手术（n = 15）。恶性肿瘤26例，良性肿瘤23例。从所有样品中提取DNA和RNA，并使用Agilent tapstation评估其产量和质量。肿瘤细胞含量计算为恶性细胞与良性细胞的比值。结果：恶性肿瘤中DNA和RNA的产率明显高于良性肿瘤（p200核苷酸（DV200））。结论：本研究结果提示细胞学样本中提取的DNA具有高度稳定性，而RNA受冷缺血时间的影响。因此，收集后及时固定是保持RNA质量的必要条件。

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引用次数: 0