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Application of activated charcoal and nanocarbon to callus induction and plant regeneration in aromatic rice (Oryza sativa L.) 活性炭和纳米炭在芳香水稻愈伤组织诱导和植株再生中的应用
Q3 Chemical Engineering Pub Date : 2018-01-01 DOI: 10.1080/09542299.2017.1418184
S. Chutipaijit, T. Sutjaritvorakul
Abstract The investigations of nanotechnology with the application on agricultural products also have been few reported, especially the plant regeneration. The effects of activated charcoal and nanocarbon on the callus induction and plant regeneration of aromatic rice were studied. Activated charcoal was added into the callus induction and regeneration medium. The presence of activated charcoal in the callus induction medium (100–500 mg L−1), activated charcoal significantly reduced the percentage of the callus induction and biomass accumulation (fresh weight, dry weight and size). Whereas, the regeneration medium supplemented with 100 mg L−1 of activated charcoal showed the highest percentage of plant regeneration (61.90%) and the ratio of the number of seedlings to the number of regenerated calli (RSR; 3.06) that derived from the callus induction medium (without activated charcoal). Moreover, the induced calli derived from the callus induction medium supplemented with nanocarbon at 5 mg L−1 showed the highest percentage of callus induction (94.70%), the percentage of green spots (95.83%), the percentage of plant regeneration (60.42%) and the RSR (3.12) when transferred the calli into the regeneration medium (without nanocarbon). After that, nanocarbon was also added into the regeneration medium. The percentage of green spots (96.08%), the percentage of plant regeneration (62.75%) and the RSR (3.16) obtained from the regeneration medium supplemented with 20 mg L−1 of nanocarbon showed the highest values. This experiment showed that the optimum concentration of activated charcoal and nanocarbon had potential to enhance the callus induction and plant regeneration frequencies in tissue culture medium of aromatic rice.
摘要纳米技术在农产品上的应用研究也很少,尤其是在植物再生方面。研究了活性炭和纳米炭对香稻愈伤组织诱导和植株再生的影响。在愈伤组织诱导再生培养基中加入活性炭。在愈伤组织诱导培养基中添加活性炭(100-500 mg L−1),活性炭显著降低了愈伤组织诱导率和生物量积累(鲜重、干重和大小)。而在添加100 mg L−1活性炭的再生培养基中,植株再生率最高(61.90%),幼苗数与再生愈伤组织数(RSR)之比最高;3.06),来源于愈伤组织诱导培养基(不含活性炭)。在不添加纳米碳的愈伤组织诱导培养基中,添加5 mg L−1纳米碳的愈伤组织诱导率最高(94.70%),绿斑率最高(95.83%),植株再生率最高(60.42%),RSR为3.12。然后,在再生培养基中加入纳米碳。在添加20 mg L−1纳米碳的再生培养基中,绿斑率(96.08%)、植株再生率(62.75%)和RSR(3.16)最高。本试验表明,活性炭和纳米炭的最佳浓度对芳香稻愈伤组织诱导和植株再生频率有促进作用。
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引用次数: 21
Spatial and temporal trends in total organic carbon (TOC), black carbon (BC), and total nitrogen (TN) and their relationships under different planting patterns in a restored coastal mangrove wetland: case study in Fujian, China 不同种植模式下滨海红树林湿地总有机碳(TOC)、黑碳(BC)和总氮(TN)的时空变化趋势及其关系——以福建为例
Q3 Chemical Engineering Pub Date : 2018-01-01 DOI: 10.1080/09542299.2018.1484673
P. Guo, Yinshi Sun, H. Su, Meixian Wang, Yuxuan Zhang
ABSTRACT This study aims to determine the sediment changes and the trends in TOC, BC and TN before and after restoration of the mangrove wetland in the Jinjiang Estuary and to determine the effect of the wetland restoration process on the biogeochemical cycle of carbon and nitrogen. The results suggest that the sediments were mainly silt-sized. Among different sites with different types of plants and vegetation densities, the adsorptive ability of N in the plots in plantations of Kandelia obovata, Avicennia marina and Acanthus ilicifolius was the highest. The TOC content differed (p < 0.05) with the density of the plot and significantly differed (p < 0.01) with the mangrove species at the densities of 0.5 × 1 m and 0.5 × 0.5 m. There was a positive relationship between the TOC and TN and the TOC and carbon-nitrogen ratio (C/N) (P < 0.05).
摘要本研究旨在研究晋江口红树林湿地恢复前后沉积物的变化及TOC、BC、TN的变化趋势,并探讨湿地恢复过程对碳氮生物地球化学循环的影响。结果表明,沉积物以粉砂质为主。在不同植物类型和植被密度的不同立地中,倒树花、牛油果和刺槐人工林样地对氮的吸附能力最高。TOC含量随样地密度差异显著(p < 0.05),在0.5 × 1 m和0.5 × 0.5 m密度下与红树林种间差异显著(p < 0.01)。TOC与TN、TOC与碳氮比(C/N)呈显著正相关(P < 0.05)。
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引用次数: 9
Trace metal speciation in a wastewater wetland and its bioaccumulation in tilapia Oreochromis niloticus 污水湿地中微量金属形态及其在罗非鱼体内的生物积累
Q3 Chemical Engineering Pub Date : 2018-01-01 DOI: 10.1080/09542299.2018.1452635
G. Aldana, M. Hernández, S. Cram, O. Arellano, O. Morton, C. A. Ponce de León
Abstract Trace metal species in the water column of a canal system forming a wetland filled with wastewater were analyzed to determine their correlation with metal accumulation in the gills of locally fished Oreochromis niloticus. The metal concentrations in the suspended particles and water dissolved were analyzed. The metal species were calculated using Windermere Humic Aqueous Model version VII showing that the high organic matter and major cation contents were important parameters. Also, the free ion metal concentration was expected to correlate with the organic matter aromaticity; however, organic compounds other than humic susbtances seem to be complexing the metals in the system. Additionally, no clear correlation could be found between metal accumulation in gills and any of the dissolved metal species. Nonetheless, certain trends could be seen between the calculated metal species and metal accumulation in the tilapia from the suspended particles.
摘要分析了形成废水湿地的运河系统水柱中的微量金属物种,以确定它们与当地捕捞的尼罗罗非鱼鳃中金属积累的相关性。分析了悬浮颗粒和溶解水中的金属浓度。使用Windermere Humic Water Model version VII计算金属物种,表明高有机物和主要阳离子含量是重要参数。此外,预计游离离子金属浓度与有机物的芳香性相关;然而,腐殖酸以外的有机化合物似乎与系统中的金属络合。此外,在鳃中的金属积累和任何溶解的金属物种之间都没有发现明确的相关性。尽管如此,在计算的金属物种和悬浮颗粒在罗非鱼中的金属积累之间可以看到某些趋势。
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引用次数: 5
Thallium pollution and potential ecological risk in the vicinity of coal mines in Henan Province, China 河南煤矿附近铊污染及其潜在生态风险
Q3 Chemical Engineering Pub Date : 2018-01-01 DOI: 10.1080/09542299.2018.1513820
Chengli Zhang, S. Ren, Hongdan Cheng, Weiping Zhang, Jianhua Ma, Chaosheng Zhang, Zhiyong Guo
ABSTRACT This study was aimed to analyze the thallium pollution and assess the potential ecological risks in the vicinity of coal mines in Henan province, China. We studied 90 surface farmland soil samples from 9 representative coal mines. The Tl concentrations were determined and the potential ecological risks were evaluated. Investigations revealed the farmland soils were modestly contaminated and the trace elements in coal mining areas transferred to the surface soils. Soil Tl contents and potential ecological risks in coal mining areas were significantly increased compared with the original soils which came from the villagers’ mud houses built 40 years ago. The soil Tl concentrations ranged from 0.25 to 0.77(mean = 0.46) mg∙kg−1, which were higher than the original level (0.42 mg∙kg−1). The potential ecological risk index of Tl ranged from 24.00 to 73.2 (mean 44.08), representing a moderate pollution level as a whole of the soils in Henan. In general, high Tl concentrations and high potential ecological risk were found around SHQ and DTG. The soil Tl concentrations exceed the original level and pose noticeable ecological risks.
摘要本研究旨在分析河南省煤矿周边地区铊污染状况,并对其潜在生态风险进行评价。研究了9个代表性煤矿90个表层农田土壤样品。测定了Tl浓度,并对潜在生态风险进行了评价。调查结果表明,矿区农田土壤受到轻度污染,矿区微量元素向表层土壤转移。矿区土壤Tl含量和潜在生态风险较40年前村民泥屋原土显著增加。土壤Tl浓度在0.25 ~ 0.77(平均0.46)mg∙kg - 1之间,高于原始水平(0.42 mg∙kg - 1)。土壤的潜在生态风险指数在24.00 ~ 73.2之间(平均44.08),总体处于中等污染水平。总体而言,SHQ和DTG附近存在高Tl浓度和高潜在生态风险。土壤Tl浓度超过原值,具有明显的生态风险。
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引用次数: 11
Combined effects of straw-derived biochar and bio-based polymer-coated urea on nitrogen use efficiency and cotton yield 秸秆生物炭和生物基聚合物包膜尿素对氮素利用效率和棉花产量的联合效应
Q3 Chemical Engineering Pub Date : 2018-01-01 DOI: 10.1080/09542299.2018.1518730
Xiaojing Yu, Xiaofei Tian, Yanyan Lu, Zhiguang Liu, Yanle Guo, Jianqiu Chen, Chengliang Li, Min Zhang, Y. Wan
ABSTRACT The interactive effects of straw-derived biochar and bio-based polymer-coated urea (BPCU) was examined with a pot experiment conducted in 2014 and 2015. Using a split-plot design, the main plot factor was the form of straw use and the sub-plot factor was the type of N fertilizer. The soil inorganic nitrogen (N), organic carbon and lint yield of biochar treatments were significantly higher than for straw treatments. Meanwhile, the BPCU treatments enhanced nitrogen use efficiency (NUE) and yield over urea treatments. Biochar combined with BPCU resulted in the highest lint yield, 14.3–108.2% increasing over the other treatments, with NUE 27.1–63.5% increased. We attributed this superior performance to the interactive effects between BPCU’s controlled supply of N according to cotton’s N requirements and biochar’s functionalities in enhancing soil quality. Thus, the application of biochar and BPCU is a sustainable strategy to improve soil quality and increase cotton yield.
摘要通过2014年和2015年的盆栽试验,研究了秸秆生物炭和生物基聚合物包膜尿素(BPCU)的相互作用。采用分块设计,主要小区因子为秸秆利用形式,次要小区因子为氮肥类型。生物炭处理的土壤无机氮、有机碳和皮棉产量显著高于秸秆处理。同时,与尿素处理相比,BPCU处理提高了氮利用效率和产量。生物炭与BPCU组合的皮棉产量最高,比其他处理增加14.3-108.2%,NUE增加27.1-63.5%。我们将这种优异的性能归因于BPCU根据棉花的氮需求控制的氮供应与生物炭在提高土壤质量方面的功能之间的相互作用。因此,应用生物炭和BPCU是改善土壤质量和提高棉花产量的可持续策略。
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引用次数: 10
Characteristics of nutrient release from sediments under different flow conditions 不同流动条件下沉积物养分释放特征
Q3 Chemical Engineering Pub Date : 2017-01-01 DOI: 10.1080/09542299.2017.1320950
Zhongbo Yu, Xiaoyang Zhong, Cong-rong Yu, Chuanhai Wang, Peiyi Duan, Lei Wen, Yang You
Abstract Contaminated sediments, as a secondary pollution source in rivers and lakes, are of critical importance to water quality. More and more attention thus has been paid to understand the release mechanisms of nutrients from river sediments, especially in estuary and water transfer areas. In this work, flume experiments were conducted to measure the release characteristics of total dissolved phosphorus (TDP) and nitrogen (TDN) from sediments collected from a river bed near Lake Tai under various flow conditions. The release of TDP and TDN was the most dramatic in the initial 30 min, then slowed down from 30 to 60 min, and finally achieved equilibria. Total amount of TDP and TDN released and their equilibrium concentrations were all significantly increased with the increase of flow rate, but slow down after a critical velocity was reached, which could be described as a Logarithmic relationship. A process-based mathematical model was established to describe the distribution of nutrients in the water columns and model simulations matched experimental data well. The re-suspension of sediments induced by flow rate higher than the threshold, is the dominant process affecting nutrient release from sediments.
污染沉积物作为河流和湖泊的二次污染源,对水质有着至关重要的影响。因此,河流沉积物中营养物质的释放机制越来越受到人们的关注,特别是在河口和调水区。通过水槽实验,研究了太湖附近河床沉积物在不同流量条件下的总溶解磷(TDP)和总溶解氮(TDN)的释放特征。TDP和TDN的释放在前30min最为剧烈,在30 ~ 60min逐渐减缓,最终达到平衡。TDP和TDN释放总量及其平衡浓度均随流量的增加而显著增加,但在达到临界流速后下降,呈对数关系。建立了一个基于过程的数学模型来描述水体中营养物的分布,模型模拟结果与实验数据吻合较好。流速大于阈值引起的沉积物再悬浮是影响沉积物养分释放的主要过程。
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引用次数: 4
Solid phase speciation of Zn and Cd in zinc smelter effluent-irrigated soils 锌冶炼废水灌溉土壤中锌和镉的固相形态
Q3 Chemical Engineering Pub Date : 2017-01-01 DOI: 10.1080/09542299.2016.1247656
P. Ray, S. Datta
Abstract Solubility of metal in contaminated soils is a key factor which controls the phytoavailability and toxic effects of metals on soil environment. The chemical equilibria of metal ions between soil solution and solid phases govern the solubility of metals in soil. Hence, an attempt was made to identify the probable solid phases (minerals), which govern the solubility of Zn2+ and Cd2+ in zinc smelter effluent-irrigated soils. Estimation of free ion activities of Zn2+ (pZn2+) and Cd2+ (pCd2+) by Baker soil test indicated that metal ion activities were higher in smelter effluent-irrigated soils as compared to that in tubewell water-irrigated soils. Identification of solid phases further reveals that free ion activity of Zn2+ and Cd2+ in soil highly contaminated with Zn and Cd due to long-term irrigation with zinc smelter effluent is limited by the solubility of willemite (Zn2SiO4) in equilibrium with quartz and octavite (CdCO3), respectively. However, in case of tubewell water-irrigated soil, franklinite (ZnFe2O4) in equilibrium with soil-Fe and exchangeable Cd are likely to govern the activity of Zn2+ and Cd2+ in soil solution, respectively. Formation of highly soluble minerals namely, willemite and octavite indicates the potential ecological risk of Zn and Cd, respectively in smelter effluent irrigated soil.
摘要金属在污染土壤中的溶解度是控制金属对土壤环境的植物有效性和毒性作用的关键因素。金属离子在土壤溶液和固相之间的化学平衡决定了金属在土壤中的溶解度。因此,试图确定可能的固相(矿物),其控制锌冶炼厂废水灌溉土壤中Zn2+和Cd2+的溶解度。通过Baker土壤试验估算Zn2+(pZn2+)和Cd2+(pCd2+)的自由离子活性表明,与管井灌溉土壤相比,冶炼厂废水灌溉土壤中的金属离子活性更高。固相鉴定进一步表明,由于锌冶炼厂废水的长期灌溉,在被锌和镉高度污染的土壤中,Zn2+和Cd2+的自由离子活性分别受到Willimite(Zn2SiO4)与石英和八方辉石(CdCO3)平衡的溶解度的限制。然而,在管井灌溉土壤的情况下,与土壤Fe和可交换Cd平衡的franklinite(ZnFe2O4)可能分别控制土壤溶液中Zn2+和Cd2+的活性。高可溶性矿物willite和octavite的形成分别表明了锌和镉在冶炼厂废水灌溉土壤中的潜在生态风险。
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引用次数: 9
Application of stable isotopes to examine N proportions within a simulated Aegiceras corniculatum wetland 应用稳定同位素检测模拟飞花Aegiceras湿地中氮的比例
Q3 Chemical Engineering Pub Date : 2017-01-01 DOI: 10.1080/09542299.2017.1339573
S. Miao, Weilin Chen, W. Tao, Wen-Hong Dai, Liandi Long, Jinling Huang
Abstract Salinity levels and drought status of coastal wetlands may be strongly affected by climate change, and changes in the nitrogen cycle of mangrove wetlands may also be affected. We established combinations of three salinity and water levels with applied stable isotope 15N to study the δ15N distributions in the sediment and plants of a greenhouse-based simulated mangrove Aegiceras corniculatum wetland system. The stable isotope 13C and 15N, C and N contents and the C:N ratio were determined. Results showed that increasing in salinity significantly increased the δ13C value in plant organs. The δ15N value of plant organs increased with increasing water level in low salinity (10‰) and medium salinity (20‰) treatment groups but not in the high salinity (30‰) treatment group. This may attributed to A. corniculatum adjusting the δ15N distribution in different organs in response to high salinity stress. Compared to the δ13C, the δ15N values of plant were strongly affected by salinity and water level treatments, indicating that the behavior of N cycle was somewhat different than the C cycle, and affected by the combined effects of both salinity and water level. Most of 15N absorbed by plant tissues were in leaves except for the highest salinity and high water level treatment, showing at increasing water level, the proportion of 15N increased in root. Overall, the measured indicators exhibited different responses to salinity level and water level, suggesting that the changes in salinity and water levels have an impact on N cycling processes of wetland systems.
摘要沿海湿地的盐度和干旱状况可能受到气候变化的强烈影响,红树林湿地氮循环的变化也可能受到影响。我们建立了三种盐度和水位与应用稳定同位素15N的组合,以研究δ15N在基于温室的模拟红树林Aegiceras corniculatum湿地系统沉积物和植物中的分布。测定了稳定同位素13C和15N、C和N的含量以及C:N比。结果表明,盐度的增加显著提高了植物器官中δ13C的值。低盐度(10‰)和中盐度(20‰)处理组植物器官的δ15N值随水位的升高而增加,而高盐度(30‰)处理则不增加。这可能归因于A.corniculatum在高盐度胁迫下调节δ15N在不同器官中的分布。与δ13C相比,植物的δ15N值受到盐度和水位处理的强烈影响,表明N循环的行为与C循环有所不同,并受到盐度和水平的综合影响。除最高盐度和高水位处理外,植物组织吸收的15N大部分在叶片中,表明随着水位的升高,15N在根部的比例增加。总体而言,测量的指标对盐度和水位表现出不同的响应,表明盐度和水位的变化对湿地系统的氮循环过程有影响。
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引用次数: 4
Spatial distribution of total halogenated organic compounds (TX), adsorbable organic halogens (AOX), and heavy metals in wetland soil irrigated with pulp and paper wastewater 纸浆造纸废水灌溉湿地土壤中总卤化有机化合物(TX)、可吸附有机卤素(AOX)和重金属的空间分布
Q3 Chemical Engineering Pub Date : 2017-01-01 DOI: 10.1080/09542299.2016.1252692
Liqiang Cui, Tianming Chen, C. Ding, Zhaoxia Li, Jinlong Yan, Yang Liu, Xian Niu, Aihui Chen, Wenjun Yang
Abstract Long-term irrigation using wastewater from paper industry may cause seriously problems to the receiving soil. This work surveyed and monitored the soil quality of a wastewater irrigation wetland system in Yancheng City, Jiangsu Province in China in 2014 and 2015. Τhe wetland soil showed different soil properties and TX, AOX, heavy metal contents after long-term wastewater irrigation. Long-term irrigation also accumulated the heavy metals such as Cu, Cd, Zn, and Pb in the wetland soil. Compared to the control, TX in the irrigated soil increased by 47.7–69.8% (2014) and 61.5–83.1% (2015). AOX varied in concentration from 1.7 to 55.0 mg kg−1 (2014) and 11.0 to 53.0 mg kg−1 (2015). The long-term irrigation of wastewater to wetland systems caused the accumulations of heavy metals, TX, and AOX in the soil and the levels of accumulations were related to several factors including soil properties, wastewater quality, and irrigation time.
摘要长期利用造纸废水进行灌溉,会对受水土壤造成严重的污染。本研究于2014年和2015年对江苏省盐城市污水灌溉湿地系统土壤质量进行了调查和监测。Τhe湿地土壤经长期废水灌溉后呈现出不同的土壤性质和TX、AOX、重金属含量。长期灌溉还积累了湿地土壤中的Cu、Cd、Zn、Pb等重金属。与对照相比,灌溉土壤TX分别增加了47.7 ~ 69.8%(2014年)和61.5 ~ 83.1%(2015年)。AOX的浓度变化范围为1.7 - 55.0 mg kg - 1(2014年)和11.0 - 53.0 mg kg - 1(2015年)。长期向湿地系统灌溉废水导致土壤中重金属、TX和AOX的积累,其积累水平与土壤性质、废水水质和灌溉时间等因素有关。
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引用次数: 13
Speciation and dynamics of phosphorus in some organically amended soils of southwestern Nigeria 尼日利亚西南部有机改良土壤中磷的形态和动态
Q3 Chemical Engineering Pub Date : 2017-01-01 DOI: 10.1080/09542299.2017.1287549
A. Soremi, M. Adetunji, J. Azeez, C. Adejuyigbe, J. Bodunde
Abstract Availability of soil phosphorus (P) is a function of its dynamics and can be improved by using organic amendments. Experiments were carried out to determine the effect of poultry manure (PM) on soil P fractions and bioavailability to soybean (Glycine max). Soils from ten farmers’ fields in Southwest Nigeria, were used for incubation and pot experiments. Treatments were five rates of PM (0, 2.5, 5.0, 7.5 and 10 t ha−1). Triplicate units in incubation and pot experiments were arranged in Completely Randomised Design. Soybean was grown for three consecutive growth cycles of seven weeks each and soil samples were analysed for Saloid P, Al-P, Fe-P, Ca-P, occluded P, reductant soluble P, and residual P. Results indicated that Fe-P was the dominant active inorganic P in the soils while residual P was the dominant inactive inorganic-P in most of the soils. In the pot experiment, application of poultry manure significantly increased organic P in the soils in the first growth cycle, but decreased slightly in second growth cycle. Highest and lowest (P ≤ 0.05) organic P values were observed in soil samples from Ayetoro and Odeda, respectively. In the pot experiment, application of PM reduced P fixation and occluded P fraction in the soils. There was general increase in available P in most of the soils considered despite increase or decrease in other forms of P. Poultry manure reduced the fixation of P and release of occluded phosphorus. Generally, Poultry manure significantly improved soil P fractions and plant P tissue concentrations.
土壤磷素有效性是一个动态函数,可以通过有机改良剂来改善。本试验旨在研究禽粪(PM)对土壤磷组分和大豆(甘氨酸max)生物有效性的影响。来自尼日利亚西南部10个农民田地的土壤被用于培养和盆栽试验。5种浓度的PM处理(0、2.5、5.0、7.5和10 t / h - 1)。孵育和盆栽试验的三个重复单位采用完全随机设计。大豆连续生长3个生长周期,每个生长周期为7周,并对土壤样品进行了碱态磷、铝磷、铁磷、钙磷、封闭磷、还原性可溶性磷和残余磷的分析。结果表明,大多数土壤中铁磷是主要的活性无机磷,而残余磷是主要的非活性无机磷。在盆栽试验中,施用禽粪显著提高了第一个生长周期土壤中有机磷的含量,但在第二个生长周期中略有下降。Ayetoro和Odeda土壤样品有机磷含量最高,P≤0.05。在盆栽试验中,施用PM降低了土壤的固磷和封闭的磷组分。尽管其他形式的磷有增减,但大多数土壤的速效磷总体呈增加趋势。禽粪降低了磷的固定和封闭磷的释放。总体而言,禽畜粪便显著提高了土壤磷组分和植物磷组织浓度。
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引用次数: 12
期刊
Chemical Speciation and Bioavailability
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