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INFECCIÓN NATURAL POR Trypanosoma cruzi (TRYPANOSOMATIDAE) EN TRIATOMINOS INTRADOMÉSTICOS DEL DEPARTAMENTO DE GUAINÍA
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-08-25 DOI: 10.15446/ABC.V26N1.84343
Cesil Alfonso Solis Medina, S. Zuluaga, Omar Triana-Chávez, Omar Cantillo-Barraza
The continuous search and characterization of triatomine bugs is essential for Chagas disease surveillance programs in areas with ideal ecological conditions for the distribution of these vectors. These activities are necessary to define and optimize intervention strategies. The objective of this work was to determine the presence of triatomine and its infection status in households located in the municipality of Inirida (Guainia). Between 2018 and 2019, we developed a community participation strategy where an active search was essential for the capturing of triatomine bugs. The collected bugs were evaluated by PCR allowing to identify one as  Panstrongylus lignarius  and four as  P. geniculatus . Genotyping showed the presence of TcI Dom confirming infection in both species. Thus, this study presents an update of the infection status of  P. geniculatus and the presence of infected P. lignarius in the Guainia region in Colombia.
在具有理想生态条件的地区,对锥蝽蝽进行持续搜索和鉴定对于恰加斯病监测项目至关重要。这些活动对于确定和优化干预策略是必要的。这项工作的目的是确定伊尼里达市(几内亚)家庭中是否存在牛角锥虫及其感染状况。在2018年至2019年期间,我们制定了一项社区参与战略,其中积极搜索对于捕获三角蝽虫至关重要。经PCR鉴定,1只为木质素Panstrongylus lignarius, 4只为P. geniculatus。基因分型显示存在TcI Dom,证实两种动物均有感染。因此,这项研究提出了一个更新的感染状况的假假木质素和存在的感染假假木质素在哥伦比亚的几内亚地区。
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引用次数: 1
CAMBIOS EN DIVERSIDAD Y DISTRIBUCIÓN DE PECES NATIVOS CON LA PRESENCIA DE DOS ESPECIES INVASORAS EN EL RÍO ATACAMES, NOROCCIDENTE DEL ECUADOR 厄瓜多尔西北部阿塔卡梅斯河本地鱼类多样性和分布的变化与两种入侵物种的存在
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-08-25 DOI: 10.15446/ABC.V26N1.81888
Pedro Jorge Jiménez Prado, Fernando Welington Vásquez Galarza
The interpretation of biological diversity can be very complex, as it exists a dynamic based on the origin and diversification of species, in addition to anthropogenic effects, such as the introduction of invasive species, which can result in the local extinction of the native ones. The aim of this study was to analyze the temporal variation (2012 and 2017),  in composition and distribution of the ichthyofauna along the Atacames River in northwestern Ecuador. In 2012 we recorded: 1) the presence of  Astyanax ruberrimus , which was reported locally extinct by 2017; and 2) the tilapia ( Orechromis niloticus ) as the only introduced fish species present until 2017, when the addition of the non-native guppy ( Poecilia gillii ) occurred . In addition, the increase in human activities conduced to change in diversity, abundance and distribution of fish along the watercourse, which resulted in dispersing of some species towards the upper reaches of the river basin.
生物多样性的解释可能非常复杂,因为它存在一个基于物种起源和多样化的动态,除了人为影响,如入侵物种的引入,可能导致本地物种的局部灭绝。本研究的目的是分析厄瓜多尔西北部阿塔卡梅斯河沿岸鱼系动物组成和分布的时间变化(2012年和2017年)。在2012年,我们记录了:1)Astyanax ruberrimus的存在,据报道,到2017年,该物种在当地已经灭绝;2)罗非鱼(Orechromis niloticus)是唯一引进的鱼类,直到2017年加入了非本地的孔雀鱼(Poecilia gillii)。此外,人类活动的增加导致河道鱼类的多样性、丰度和分布发生变化,导致部分物种向流域上游分散。
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引用次数: 1
Coordinating invasive alien species management in a biodiversity hotspot: The CAPE Invasive Alien Animals Working Group 在生物多样性热点地区协调外来入侵物种管理:CAPE外来入侵动物工作组
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-08-19 DOI: 10.38201/BTHA.ABC.V50.I1.10
S. Davies, Jonathan A. Bell, D. Impson, C. Mabin, Marco Meyer, Chandre Rhoda, Louise Stafford, Kirstin Stephens, Mfundo Tafeni, A. Turner, Nicola J. van Wilgen, John R. U. Wilson, J. Wood, J. Measey
Background: The effectiveness of invasive alien species management in South Africa, and elsewhere, can be mproved by ensuring there are strong links and feedbacks between science and management. The CAPE Invasive Alien Animals Working Group (CAPE IAAWG) was established in 2008 to enhance cooperation among stakeholders such as implementing agencies and researchers, and thereby improve the management of invasive animals in the Greater Cape Floristic Region.Objectives: In this article we highlight where and how the working group has advanced our understanding of research and the implementation of management objectives and consider the working group’s successes and failures.Methods: We analyse the attendance of meetings by different stakeholders and the frequency of discussion topics on meeting agendas throughout the sequence of meetings from 2008 to 2019. We document insights based on published accounts or the experiences of the authors from eight different management projects.Results: Meetings are attended by stakeholders from NGOs, universities, and local, provincial and national government agencies as well as private individuals. Topics of discussion ranged from details of specific alien animal invasions (e.g. the House Crow in Cape Town), to considering the risks posed by broad groups (e.g. earthworms), to specific management techniques (e.g. guidelines for trapping invasive alien birds). Through the eight projects described here the CAPE IAAWG has: (i) contributed to capacity building through funding and advising on post-graduate research projects; (ii) provided ad hoc support to staff of agencies that implement invasive alien animal control; (iii) acted as a focal point for a community of practice that is supportive of decision making and policy development; and (iv) played a vital role in linking research, management and policy in a manner accessible to a broader range of stakeholders. The projects undertaken by the group reveal several lessons for managing invasive animals: (i) the importance of logistics and contract efficiency, (ii) the need for effective stakeholder engagement by the project team, (iii) the need to effectively address conflicts between role players, and (iv) the importance of including ethical and animal rights considerations in the decision making processes.Conclusion: The CAPE IAAWG has been a valuable forum to improve management effectiveness and support implementation decisions. Due to its small cost and time footprint, the working group has remained viable and retained a core of committed members, ensuring ongoing institutional buy-in. The working group will remain successful so long as the group is supported by its members and their organisations.
背景:通过确保科学与管理之间的紧密联系和反馈,可以提高南非和其他地方外来入侵物种管理的有效性。CAPE外来入侵动物工作组(CAPE IAAWG)成立于2008年,旨在加强实施机构和研究人员等利益相关者之间的合作,从而改善大开普植物区入侵动物的管理。目标:在本文中,我们强调了工作组在哪里以及如何提高了我们对研究和管理目标实施的理解,并考虑了工作组的成功和失败。方法:我们分析了2008年至2019年整个会议序列中不同利益相关者的会议出席率和会议议程讨论主题的频率。我们根据8个不同管理项目的作者发表的文章或经验来记录见解。结果:来自非政府组织、大学、地方、省和国家政府机构以及个人的利益相关者参加了会议。讨论的主题从特定外来动物入侵的细节(例如开普敦的家鸦),到考虑广泛群体(例如蚯蚓)构成的风险,再到具体的管理技术(例如捕获入侵外来鸟类的指南)。通过上述八个项目,CAPE IAAWG:(i)通过资助研究生研究项目并为其提供咨询意见,促进了能力建设;(ii)向实施外来入侵动物控制的机构的工作人员提供特别支助;(iii)作为支持决策和政策制定的实践社区的联络点;(iv)在以更广泛的利益攸关方可获得的方式将研究、管理和政策联系起来方面发挥了至关重要的作用。该小组开展的项目揭示了管理入侵动物的几个经验教训:(i)物流和合同效率的重要性,(ii)项目团队有效参与利益相关者的必要性,(iii)有效解决角色参与者之间冲突的必要性,以及(iv)在决策过程中考虑道德和动物权利因素的重要性。结论:CAPE IAAWG已成为提高管理有效性和支持实施决策的重要论坛。由于其成本和时间占用较小,工作组仍然可行,并保留了一批忠诚的核心成员,确保了机构的持续支持。只要工作组得到其成员及其组织的支持,工作组就会保持成功。
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引用次数: 3
Distribution of Mimosa diplotricha in eastern and southern Africa and its socioecologicalimpacts in northern Malawi 含羞草在非洲东部和南部的分布及其对马拉维北部的社会生态影响
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-07-30 DOI: 10.38201/btha.abc.v50.i1.9
A. Witt, Lilian Chimphepo, T. Beale, Winnie Nunda
Background: Mimosa diplotricha is an emerging or established weed in manyparts of the world, including many countries in Africa, where it is impacting onbiodiversity, crop and pasture production, and driving socio-ecological change.Objectives: To establish the current distribution of M. diplotricha in eastern andsouthern Africa and its impacts on livelihoods in northern Malawi.Methods: Records on current distribution were collected from roadside surveys,literature reviews and herbarium data. Household surveys were conducted in theKaronga District, Malawi, to understand its impacts on local livelihoods.Results: Mimosa diplotricha is abundant in western Ethiopia, southern Tanzania,and northern and southeastern Malawi with isolated populations in westernRwanda, Burundi, Mozambique, and on the northern shores of Lake Victoria inUganda. Most respondents said that M. diplotricha invasions were reducing theamount of grass and shrubs in rangelands, with over half saying it reduced cropyields. This invasive plant is also reducing the availability of medicinal plants andother natural resources.Conclusions: Mimosa diplotricha has the potential to significantly expand itsrange in eastern Africa, and parts of southern Africa, and as such there is anurgent need to develop and implement an integrated management strategy, includingbiological control, to reduce the negative effects of this invasive plant onlocal livelihoods.
含羞草(Mimosa diplotricha)是一种新兴或成熟的杂草,在世界许多地方,包括非洲的许多国家,它正在影响生物多样性、作物和牧场生产,并推动社会生态变化。目的:确定非洲东部和南部白僵菌的分布现状及其对马拉维北部生计的影响。方法:通过路边调查、文献查阅、植物标本室资料等收集现状分布记录。在马拉维karonga区进行了住户调查,以了解其对当地生计的影响。结果:Mimosa diplotricha在埃塞俄比亚西部、坦桑尼亚南部、马拉维北部和东南部分布丰富,在卢旺达西部、布隆迪、莫桑比克和乌干达维多利亚湖北岸有孤立种群。大多数受访者表示,白桦尺蠖的入侵减少了牧场草地和灌木的数量,超过一半的受访者表示它减少了农作物产量。这种入侵植物也减少了药用植物和其他自然资源的可用性。结论:含羞草(Mimosa diplotricha)有可能在非洲东部和南部部分地区显著扩大其分布范围,因此迫切需要制定和实施包括生物防治在内的综合管理策略,以减少这种入侵植物对当地生计的负面影响。
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引用次数: 1
O ICMS Ecológico como ferramenta de conservação: estudo de caso no município de Marmeleiro (PR) 生态ICMS作为保护工具:马梅洛罗市案例研究(PR)
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-06-30 DOI: 10.21726/ABC.V7I2.150
Jonas Galleazzi Borges, Dionatan Gerber, Bruna Elisa Trentin, Fernando Campanhã Bechara
This study aimed to assess socioenvironmental impacts from implementing the Ecological ICMS Program in a riparian community – Marmeleiro (PR), Brazil –, through a quantitative questionnaire with open and closed questions. Socioenvironmental impacts were identified such as how suitable was the project to properties’ reality and the owners’ opinion about the fences implanted by the Ecologic ICMS for riparian forests protection. The program allowed the isolation of riparian forests (through fencing) and the readjustment of rural roads. The differential in the implementation of actions in this community was the community’s social capital, which has strengthened the social networks. The major community’s environmental concern was the importance relation between riparian forests and quality/quantity of water available. We detected a lack of published articles about Ecologic ICMS programs. For their implementation, it is important to identify riparian farmers’ motivations and to proceed with environmental education actions. We recommend to strength the active social networks and enhance the environmental monitoring by government agencies for the success of these programs.
本研究旨在通过包含开放和封闭问题的定量问卷,评估在巴西Marmeleiro (PR)河岸社区实施生态ICMS计划对社会环境的影响。社会环境影响被确定,例如项目是否适合物业的实际情况,以及业主对生态ICMS为保护河岸森林而植入的围栏的意见。该方案允许隔离河岸森林(通过围栏)和重新调整农村道路。该社区在行动实施方面的差异在于社区的社会资本,它加强了社会网络。社区主要关心的环境问题是河岸森林与可用水的质量/数量之间的重要关系。我们发现缺乏关于生态ICMS项目的已发表文章。为了实施这些措施,重要的是要确定沿岸农民的动机,并开展环境教育行动。我们建议加强积极的社会网络,加强政府机构的环境监测,以确保这些项目的成功。
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引用次数: 0
Fenologia de Eugenia pyriformis Cambess (uvaia) em reflorestamento no sudoeste do Paraná parana西南部造林中的pyriformis Cambess (uvaia)物候学
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-06-30 DOI: 10.21726/ABC.V7I2.149
Carla Marins Santos Santana Viana, Patrícia Ramos de Souza, M. Gorenstein, Daniela Aparecida Estevan, Fernando Campanhã Bechara
conhecimento fenológico papel fundamental na conservação de ecossistemas, comunidades e populações. Os objetivos deste estudo são monitorar e detectar as fenofases de E. pyriformis , em área de restauração florestal, no município de Dois Vizinhos, Paraná. Observaram-se ciclos fenológicos de 12 árvores matrizes, quinzenalmente, durante 24 meses. Houve correlação entre os parâmetros fenológicos vegetativos e as variáveis climáticas de temperatura local e pluviosidade, evidenciada no aumento da produção foliar em meses de temperaturas mais elevadas. Os maiores picos de brotação ocorreram no mês de outubro, variando de 67% a 83% nos anos de 2015 e 2016, respectivamente. A queda foliar mostrou-se inversamente proporcional à brotação, com maiores incidências de queda foliar no outono e no inverno, chegando a 73% em março e 75% em agosto de 2016. Em período de geadas, não houve brotação. Foram encontradas maiores incidências de brotação durante o verão e baixas ABSTRACT E. pyriformis (uvaia) is a fruit species, used in ecological restoration, urban afforestation, for medicinal purposes, food production, maintenance of wild fauna. Phenological knowledge plays a fundamental role in the conservation of ecosystems, communities and populations. The objective of this study was to monitor and detect E. pyriformis phenophases, in a forest restoration area, in the municipality of Dois Vizinhos, Paraná. Phenological cycles of 12 matrix trees were observed, fortnightly, for 24 months. There was a correlation between the vegetative phenological parameters and the climatic variables of local temperature and rainfall, evidenced by the increase in leaf production in months of higher temperatures. The biggest sprouting peaks occurred in October, varying from 67% to 83% in 2015 and 2016, respectively. Leaf fall was inversely proportional to sprouting, with higher incidences of leaf fall, in autumn and winter, reaching 73% in March, and 75% in August 2016. In frost periods, there was no sprouting. Higher incidences of sprouting were observed during the summer and lower in the winter. The leaf fall was more intense in the low temperature season. Other phenophases were not clearly observed, probably due to the early age of the plants.
物候学知识在保护生态系统、群落和种群方面发挥着关键作用。本研究的目的是监测和检测在Dois Vizinhos市森林恢复地区的梨形e . pyriformis物候期,parana。12棵母树的物候周期为24个月,每两周观察一次。营养物候参数与当地温度和降雨量等气候变量之间存在相关性,这可以从高温月份叶片产量的增加中得到证明。发芽高峰出现在10月份,2015年和2016年分别为67% ~ 83%。落叶与发芽成反比,秋冬落叶发生率较高,2016年3月和8月分别达到73%和75%。在霜冻时期,没有发芽。摘要pyriformis (uvaia)是一种用于生态恢复、城市造林、药用、食品生产、野生动物维护的水果品种,夏季出芽发生率较高。物候学知识在保护生态系统、社区和种群方面发挥着关键作用。本研究的目的是监测和检测在Dois Vizinhos市的森林恢复地区的pyriformis phenophases, parana。= =地理= =根据美国人口普查,这个县的总面积是,其中土地和(3.064平方公里)水。植物物化参数与当地温度和降雨的气候变量之间存在相关性,这可以从较高温度月份叶片产量的增加中得到证明。= =地理根据美国人口普查局的数据,这个县的总面积,其中土地和(1.2%)水。2016年3月和8月,落叶率分别为73%和75%,秋冬落叶率分别为73%和75%。在这段时间里,我不知道该怎么做。= =地理= =根据美国人口普查,该县的总面积为,其中土地和(5.5%)水。= =地理= =根据美国人口普查局的数据,该县总面积为,其中土地和(1.)水。= =地理= =根据美国人口普查,这个县的土地面积为。
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引用次数: 2
Estrutura fitossociológica da vegetação arbóreo-arbustiva em área de caatinga com histórico de perturbação antrópica na Paraíba, Brasil 巴西paraiba地区具有人为干扰历史的caatinga地区乔木-灌木植被的植物社会学结构
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-06-15 DOI: 10.21726/ABC.V7I1.506
Francisco de Assis Freitas, Alan Cauê de Holanda, P. B. Maracajá, Anderson Bruno Anacleto de Andrade, José Lucas Guilherme Santos, Flávio Sarmento de Oliveira
O bioma caatinga abrange um complexo vegetacional que reune ambientes muito distintos, fisionomias variadas e flora diversificada. A caatinga, mesmo com sua importância socioeconomica, e o menos protegido dentre os biomas brasileiros e sua degradacao pela acao antropica acaba por comprometer cada vez mais os recursos naturais e a sustentabilidade de tal bioma. Nesse sentido, objetivou-se realizar um levantamento da estrutura da vegetacao arboreo-arbustiva de uma area de caatinga no municipio de Vieiropolis, no estado da Paraiba. Para amostragem do componente adulto, instalaram-se 30 unidades amostrais de 20 m x 20 m de forma sistematica, onde foram inventariados todos os individuos com CAP ≥ 6,0 cm. No total, inventariaram-se 1.627 individuos, distribuidos em 16 especies e 10 familias botânicas. O indice de diversidade calculado foi de 1,18 nats.individuo-1. O alto numero de especies tipicas dos estagios iniciais da sucessao e a baixa riqueza floristica sugerem que a area estudada esta em um estagio inicial de sucessao ecologica, mesmo apos mais de tres decadas sem exploracao.
caatinga生物群落包括一个植被复合体,它聚集了非常不同的环境,不同的地貌和不同的植物群。尽管卡廷加具有重要的社会经济意义,是巴西生物群落中受保护最少的生物群落,人类活动对其退化的影响越来越大,最终损害了该生物群落的自然资源和可持续性。在这个意义上,目的是对Paraiba州Vieiropolis市caatinga地区的乔木-灌木植被结构进行调查。对成虫成分进行取样,系统安装30个20 m × 20 m的采样单元,对CAP≥6.0 cm的所有个体进行盘点。共鉴定个体1627个,分布于10科16种。多样性指数为1.18 nats. individual -1。早期传承阶段的典型物种数量高,植物区系丰富度低,表明研究区域处于生态传承的早期阶段,即使在30多年未开发之后。
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引用次数: 0
Long-term changes in forest cover in a global biodiversity hotspot in southern Mozambique 莫桑比克南部全球生物多样性热点地区森林覆盖的长期变化
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-05-19 DOI: 10.38201/btha.abc.v50.i1.1
Wataru Tokura, Hermenegildo Matimele, J. Smit, M. Hoffman
Background: Deforestation is a complex and dynamic process of widespread concern in sub-Saharan Africa which is influenced by a range of social, economic and biophysical factors.Objectives: The aim of this study was to analyse patterns of deforestation and its potential drivers in the Licuáti Forest Reserve, a biodiversity hotspot in southern Mozambique between 1990 and 2016. Method: We performed image classification on Landsat imagery at six time steps and interviewed local community members to understand the spatial pattern and rate of forest cover loss over time. We also examined changes in the incidence of fire.Results: A substantial increase in the rate of deforestation since 1990 was detected in this vulnerable thicket vegetation. The probability of deforestation was significantly higher near the major roads, where houses are located. This suggests that the proximity of human settlements to the forest, and access to charcoal markets in urban areas, influenced the spatial pattern. Two key factors 1) charcoal production, and 2) the establishment of settlements and agricultural lands were identified as proximate causes of deforestation. In addition, fires associated with these two causes might amplify the loss of forests in the area.Conclusions: Complex interactions between the drivers of deforestation and socio-economic factors were suggested, as most of the charcoal produced in the region is transported to Maputo. Ongoing road improvements and infrastructural development in the region will likely accelerate the decline in forest cover in the future. This has implications for the biodiversity of the region as well as for the sustainability of local livelihoods, as they often depend on forest products for their daily uses.
背景:森林砍伐是撒哈拉以南非洲广泛关注的一个复杂和动态的过程,受到一系列社会、经济和生物物理因素的影响。目的:本研究的目的是分析1990年至2016年间莫桑比克南部生物多样性热点Licuáti森林保护区的森林砍伐模式及其潜在驱动因素。方法:对Landsat影像进行6个时间步骤的图像分类,并对当地社区成员进行访谈,了解森林覆盖的空间格局和损失率。我们还研究了火灾发生率的变化。结果:自1990年以来,这一脆弱的灌丛植被的森林砍伐率大幅增加。在房屋所在的主要道路附近,森林砍伐的可能性要高得多。这表明人类住区与森林的接近程度以及城市地区进入木炭市场的机会影响了空间格局。两个关键因素(1)木炭生产和2)定居点和农业用地的建立被确定为森林砍伐的近因。此外,与这两个原因相关的火灾可能会加剧该地区森林的损失。结论:森林砍伐的驱动因素与社会经济因素之间存在复杂的相互作用,因为该地区生产的大部分木炭都被运往马普托。该地区正在进行的道路改善和基础设施发展可能会在未来加速森林覆盖的减少。这对该地区的生物多样性以及当地生计的可持续性都有影响,因为他们的日常生活往往依赖森林产品。
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引用次数: 3
Cultivo in vitro do cultivar italiano de morangueiro Pircinque
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-05-08 DOI: 10.21726/ABC.V7I1.648
Samila Silva Camargo, Aline Meneguzzi, L. Rufato
Estudos sobre a multiplicacao de cultivares de morangueiro recentemente introduzidos no Brasil sao necessarios para verificar o potencial de producao das mudas. Verificaram-se dois meios de cultura (KNOP e MS) e componentes, no estabelecimento e multiplicacao in vitro de Pircinque, a fim de otimizar o protocolo de micropropagacao para o cultivar. Pesquisaram-se concentracoes de PPM® durante o estabelecimento e na multiplicacao, niveis de sacarose, agar, carvao ativado, Fe EDDHa, BAP e GA3. Alem disso, foi avaliada a influencia da “dupla fase”, com combinacoes do meio de cultivo, carvao ativado e BAP. Concluiu-se que, no estabelecimento, KNOP + 2 mL L-1 PPM possibilita maior sobrevivencia dos explantes, enquanto o meio de cultura MS favorece a multiplicacao deles. A adicao dos seguintes componentes e indicada para a maior eficiencia da tecnica: 30 g L-1 sacarose; 6 g L-1 agar; 4 g L-1 carvao ativado; 0,1 g L-1 Fe EDDHA; 1 mg L-1 BAP; e 0,250 mg L-1 GA3. Apos cultivo em meio MS solido, indica-se o uso da tecnica de “dupla fase”, com adicao de MS liquido com a combinacao de 0,5 mg L-1 de BAP e de 4 g L-1 de carvao ativado.
对巴西新引进的草莓品种的繁殖进行研究是必要的,以验证幼苗的生产潜力。摘要对两种培养基(KNOP和MS)及其组分进行了培养和离体增殖试验,以优化该品种的微繁殖方案。研究了PPM®在建立和增殖过程中的浓度、蔗糖、琼脂、活性炭、EDDHa Fe、BAP和GA3的水平。此外,还评价了培养基、活性炭和BAP组合“双相”的影响。结果表明,KNOP + 2ml L- 1ppm可提高外植体的存活率,而MS培养基有利于外植体的增殖。添加以下成分表明该技术的最高效率:30 g L-1蔗糖;6 g L-1琼脂;4 g L-1活性炭;0.1 g L-1 Fe EDDHA;1 mg L-1 BAP;和0.250 mg L-1 GA3。在固体MS培养基中培养后,采用“双相”技术,加入0.5 mg L-1 BAP和4 g L-1活性炭的液体MS。
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引用次数: 0
Rápida produção de mudas de pitaia (Hylocereus undatus, Cactaceae) por meio da técnica da micropropagação 利用快繁技术快速生产火龙果幼苗(仙人掌科火龙果)
IF 0.6 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2020-05-08 DOI: 10.21726/ABC.V7I1.632
M. J. Gonçalves, S. Camargo, Ana Luiza Arruda, L. Rufato
O objetivo deste estudo foi avaliar dois meios de cultura combinados com diferentes concentracoes de BAP (6-benzilaminopurina) na propagacao in vitro de pitaia. O experimento foi realizado na Universidade do Estado de Santa Catarina. Os tratamentos constituiram-se de dois meios de cultura (MS e QL modificado) e tres concentracoes de BAP (0, 1 e 2 mg.L-1), originando um esquema fatorial 2x3 em um delineamento inteiramente casualizado com 10 repeticoes de cinco explantes. O uso do meio de cultura QL modificado na ausencia de BAP (6-benzilaminopurina) assegura media superior para a variavel numero de brotacoes. Para a producao massal de explantes de pitaia (Hylocereus undatus), o meio QL modificado sem a adicao de BAP e uma possibilidade de uso na micropropagacao, com baixo custo e rendimento superior a meios de cultura com suplementacao de BAP.
本研究的目的是评价两种不同浓度BAP(6-苄基氨基嘌呤)的培养基对火龙果离体繁殖的影响。这项实验是在圣卡塔琳娜州立大学进行的。处理包括2种培养基(MS和改良QL)和3种BAP浓度(0、1和2 mg.L-1),采用完全随机设计,10个重复,5个外植体。在没有BAP(6-苄基氨基嘌呤)的情况下使用改良的QL培养基,确保了不同芽数的优越培养基。对于火龙果外植体的批量生产,不添加BAP的改良QL培养基具有微繁殖的潜力,成本低,产量优于添加BAP的培养基。
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引用次数: 2
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Bothalia
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