Biotechnology & Genetic Engineering Reviews最新文献

We evaluate the value of progressive muscle relaxation (PMR) for patients with lumbar disc herniation after surgery based on a difference-in-differences model. A total of 128 patients with lumbar disc herniation who underwent surgery were randomly assigned to undergo either conventional intervention (conventional intervention group, n = 64) or conventional intervention combined with PMR (PMR group, n = 64). Perioperative anxiety level, stress level and lumbar function were compared between the two groups and compared pain between the two groups before and 1 week and 1 and 3 months after surgery. After 3 months, no one was lost to follow-up. At 1 day before surgery and 3 days after surgery, Self-rating Anxiety Scale score in the PMR group was significantly lower than that in the conventional intervention group (P < 0.05). At 30 min before surgery, heart rate and systolic blood pressure in the PMR group were significantly lower than those in the conventional intervention group (P < 0.05). After intervention, the scores of subjective symptoms, clinical signs and restrictions on activities of daily living were significantly higher in the PMR group than those in the conventional intervention group (all P < 0.05). Visual Analogue Scale score in the PMR group was significantly lower than that in the conventional intervention group (all P < 0.05). The amplitude of change in VAS score in the PMR group was greater than that in the conventional intervention group (P < 0.05). PMR can relieve perioperative anxiety and stress in patients with lumbar disc herniation, reduce postoperative pain and improve lumbar function.

Although miR-199a-5p is linked to the development of numerous cancers, its regulatory role in skin cancer is unclear. In this work, the impact of miR-199a-5p produced by adipose-derived stem cells on malignant melanoma skin cancer was investigated.30 pair tumor tissues and adjacent tissues were obtained from skin cancer patients. Adipose-derived stem cell (ADSCs) were isolated from adipose tissues harvested from healthy subjects. The mRNA relative expression was evaluated via qRT-PCR. Cell proliferation ability was measured via CCK-8 assay. Apoptosis was evaluated via flow cytometry. The connection between miR-199a-5p and SOX4 was confirmed via luciferase reporter assay. Western blot was conducted to evaluate protein expression. MiR-199a-5p was higher expressed in ADSCs exosomes and was lower expressed in skin cancer tissues and cells. ADSCs-derived exosomes inhibited cell invasion of skin cancer. MiR-199a-5p inhibitor enhanced cell viability and invasion. In addition, miR-199a-5p inhibitor suppressed cell apoptosis. MiR-199a-5p NC transfected ADSCs inhibited cell viability and invasion while miR-199a-5p mimic transfected ADSCs further inhibited cell viability and invasion. In addition, miR-199a-5p NC transfected ADSCs enhanced cell apoptosis while miR-199a-5p mimic transfected ADSCs further enhanced cell apoptosis. Luciferase supported the targetscan prediction that miR-199a-5p might control SOX4 expression. SOX4 expression was noticeably lower in the miR-199a-5p mimic group.Exosomes from adipose-derived stem cells inhibited skin cancer progression via miR-199a-5p/SOX4.

Acute myeloid leukemia (AML) is a common hematologic malignancy in adults. Recent studies investigating the potential pathogenesis of AML have significantly advanced our understanding of this disease. While cytogenetics and molecular abnormalities are crucial for confirming chemotherapy response and long-term outcomes, there are additional potential therapeutic targets and prognostic factors. The CAPN1 gene, which encodes a large subunit of the ubiquitous enzyme calpain, has not been extensively studied in hematological diseases. In this study, we used data from the TCGA public database to perform a bioinformatic analysis and found that CAPN1 is differentially expressed in multiple cancers and is associated with an unfavorable prognosis in AML. We employed R software and websites such as David and STRING to conduct differential analysis, GO and KEGG analysis, and explore the correlation between CAPN1 and physiological processes and key pathways. Our findings suggest that CAPN1 is significantly associated with the structure of the extracellular matrix and receptor-ligand interactions, indicating its potential role in disease progression. Additionally, we used CYBERSORT and ssGSEA to analyze the immune environment of CAPN1 and found that it is associated with most immune components, particularly CD56 cells and neutrophils. In conclusion, CAPN1 is a key prognostic gene in AML that is significantly correlated with disease progression, clinical features, and immune invasion.

Hypnotherapy combined with cognitive therapy is an effective way to intervene anxiety problems, which also responds to the call that using hypnotherapy to treat somatic disorders should become a trend in the future. This paper constructs an evaluation index of the intervention effect of cognitive hypnotherapy on anxiety patients, and then uses neural network to evaluate its effect. At last, we have completed the following work: 1) This paper constructs the theoretical basis related to this topic after searching and sorting out the related literature on anxiety disorders and hypnotherapy at home and abroad. 2) This paper constructs the evaluation index system of the intervention effect of cognitive hypnotherapy on anxiety patients, and then introduces the basic principle and structure of DBN model. 3) Experiments are used to determine the best values for the DBN model's parameters. To accomplish this, you will need to input the experimental data into the trained model and compare the evaluation results from experts with the model's predictions. The experimental findings of this study demonstrate the great accuracy of the DBN model presented in this work for assessing the effectiveness of cognitive combination hypnotherapy for anxiety disorders.

Through the analysis of the existing case data, this study explored the application effect of predictive nursing combined with bird nest nursing in children with hypoxic ischemic encephalopathy. The data of 120 children with hypoxic-ischemic encephalopathy admitted to our hospital from May 2019 to June 2021 were retrospectively analyzed, and they were divided into A (n = 40), B (n = 40) and C (n = 40) groups according to different nursing methods. Neonatal neurobehavioral assessment (NBNA), Canadian derivatives clearing corporation (CDCC) and Gesell Developmental Schedules (GDS) scores were compared among the three groups. Height, body mass, daily sleep time, milk intake and the occurrence of neurological sequelae were compared among the three groups. After intervention, the score of group A was higher than that of group B and C, with group B higher than group C (P<0.05). In addition, both mental developmental index (MDI) and psychomotive development index (PDI) of the three groups were all improved, in which Group A was the highest, followed by Group B (P<0.05).   Scores of GDS of group A and B were higher than those of group C, while GDS of group A were higher than those of group B (P<0.05).   After intervention, increases of height, body mass and milk intake in group A and B were greater than those in group C, while the increases of height and body mass in group A were better than those in group B (P<0.05).

To explore the analysis on application effect and prognostic factors of medical care combined with nursing in the elderly with type 2 diabetes mellitus (T2DM) and cerebral infraction (CI) based on targeted management mode. The clinical data of 180 elderly patients with T2DM and CI in our hospital from August 2017 to August 2019 were selected for retrospective analysis. Their cognitive function and daily living ability before and after intervention were evaluated, using the National Institutes of Health Stroke Scale (NIHSS) to evaluate their prognosis. They were divided into good prognosis group (n = 134) and poor prognosis group (n = 46) according to the score. Binary Logistic regression analysis was adopted to analyze the prognostic factors of such patients. After intervention, patients had visibly lower indexes of blood glucose fluctuation and lower average scores of ADL and MMSE (P < 0.001), with differences in body mass index, systolic pressure, diastolic pressure, fasting blood glucose and triglyceride in both groups (P < 0.001). Binary Logistic regression analysis showed that systolic pressure, diastolic pressure and triglyceride were risk factors affecting patients' prognosis (P < 0.05). Medical care combined with nursing based on targeted management mode has a remarkable control effect on blood glucose, and has a positive effect on improving cognitive function and living ability of elderly patients with T2DM and CI. In addition, attention should be paid to monitoring systolic and diastolic blood pressures, and triglyceride in patients to improve the prognosis.

Objective: To explore the diagnostic value of ultrasound and nerve electromyography (EMG) in the diagnosis of carpal tunnel syndrome (CTS) and its severity in the elderly.

Methods: The data of 140 elderly CTS patients were retrospectively analyzed. The data of 80 patients with other diseases with similar symptoms and high suspicion of CTS during the same period were retrospectively analyzed. Pearson method was used to analyze the correlation between cross-sectional area (CSA) and motor nerve conduction velocity (MCV), distal motor latency (DML), compound muscle action potential (CMAP), sensory conduction velocity (SCV), middle-latency (ML) and sensory nerve action potential (SNAP) levels. The value of CSA, MCV, DML, CMAP, SCV, ML and SNAP in the diagnosis of CTS and its severity was analyzed by receiver operating characteristic (ROC) curve.

Results: CSA in mild, moderate and severe groups was positively correlated with DML (P < 0.001) and negatively correlated with CMAP (P < 0.001). The area under the curve (AUC) values of CSA, MCV, DML, CMAP, SCV, ML and SNAP in the diagnosis of normal people and mild CTS were 0.877, 0.787, 0.921, 0.730, 0.860, 0.688 and 0.904. The AUC values of CSA, DML, CMAP, SCV, ML and SNAP in the diagnosis of mild and moderate CTS were 0.863, 0.890, 0.760, 0.848, 0.850 and 0.739, respectively. The AUC values of CSA, MCV, DML and CMAP in the diagnosis of mild and moderate CTS were 0.683, 0.660, 0.870 and 0.693.

Conclusion: Ultrasound and nerve EMG are effective in the diagnosis of CTS.

Carotenoids are fat-soluble bio pigments often responsible for red, orange, pink and yellow coloration of fruits and vegetables. They are commonly referred as nutraceutical which is an alternative to pharmaceutical drugs claiming to have numerous physiological benefits. However their activity often get disoriented by photonic exposure, temperature and aeration rate thus leading to low bioavailability and bio accessibility. Most of the market value for carotenoids revolves around food and cosmetic industries as supplement where they have been continuously exposed to rigorous physico-chemical treatment. Though several encapsulation techniques are now in practice to improve stability of carotenoids, the factors like shelf life during storage and controlled release from the delivery vehicle always appeared to be a bottleneck in this field. In this situation, different technologies in nanoscale is showing promising result for carotenoid encapsulation and delivery as they provide greater mass per surface area and protects most of their bioactivities. However, safety concerns related to carrier material and process must be evaluated crucially. Thus, the aim of this review was to collect and correlate technical information concerning the parameters playing pivotal role in characterization and stabilization of designed vehicles for carotenoids delivery. This comprehensive study predominantly focused on experiments carried out in past decade explaining how researchers have fabricated bioprocess engineering in amalgamation with nano techniques to improve the bioavailability for carotenoids. Furthermore, it will help the readers to understand the cognisance of carotenoids in nutraceutical market for their trendy application in food, feed and cosmeceutical industries in contemporary era.

Acute anterior uveitis (AAU) can cause great pain to patients. Th cytokines in peripheral blood are significantly changed in these patients, including serum interleukin-23 (IL-23), interleukin-17 (IL-17), interleukin-4 (IL-4), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) increase. However, the relationship between Th cytokines and recurrence of AAU is still unclear. Ninety-two cases of AAU were enrolled from January 2020 to April 2022 in our hospital (observation group). Levels of peripheral blood Th cytokines were detected, which were compared between acute and remission stages. After 6 months of follow-up, the relationships between Th cytokines in peripheral blood and recurrence in the observation group were analyzed. The prediction of Th cytokines on recurrence was analyzed. (1) The recurrence rate was 25.00%, and there were no statistical differences in the serum IL-23, IL-17, IL-4, IFN-γ, TNF-α and TGF-β 1 levels between patients with bilateral disease and unilateral disease (P < 0.05). (2) The serum IL-23, IL-17, IL-4, IFN-γ, TNF-α and TGF-β 1 levels of recurrence patients were higher than those of non-recurrence (t = 2.971, 5.357, 2.197, 2.766, 4.395, 2.983, P < 0.05). (3) The serum IL-23, IL-17, TNF-α levels were risk factors for recurrence (OR = 1.035, 1.210, 1.155, P < 0.05). (4) There were positive relationships between serum IL-23, IL-17, IL-4, IFN-γ, TNF-α and TGF-β 1 levels and recurrence (r = 0.317, 0.526, 0.248, 0.304, 0.480, 0.325, P < 0.05).            .

Secondary metabolites made by plants and used through their metabolic routes are today's most reliable and cost-effective way to make pharmaceuticals and improve health. The concept of genetic engineering is used for molecular pharming. As more people use plants as sources of nanotechnology systems, they are adding to this. These systems are made up of viruses-like particles (VLPs) and virus nanoparticles (VNPs). Due to their superior ability to be used as plant virus expression vectors, plant viruses are becoming more popular in pharmaceuticals. This has opened the door for them to be used in research, such as the production of medicinal peptides, antibodies, and other heterologous protein complexes. This is because biotechnological approaches have been linked with new bioinformatics tools. Because of the rise of high-throughput sequencing (HTS) and next-generation sequencing (NGS) techniques, it has become easier to use metagenomic studies to look for plant virus genomes that could be used in pharmaceutical research. A look at how bioinformatics can be used in pharmaceutical research is also covered in this article. It also talks about plant viruses and how new biotechnological tools and procedures have made progress in the field.