Gene Expression Patterns最新文献_第2页

Expression analysis of amphioxus orthologues of genes expressed in vertebrate lateral plate or pharyngeal mesoderm 文昌鱼同源基因在脊椎动物侧板或咽中胚层的表达分析。

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2025-06-13 DOI: 10.1016/j.gep.2025.119397

Anaël Soubigou , Lydvina Meister , Lucie Subirana , Lucille Cornand , Hector Escriva , Stéphanie Bertrand

The lateral plate mesoderm of vertebrates, which borders the other mesodermal territories, develops during embryogenesis into a variety of tissues and organs such as blood, heart, vasculature, kidney and smooth muscles. This mesoderm compartment, as well as the unsegmented pharyngeal mesoderm which gives rise to head muscles and part of the heart, have been proposed as vertebrate innovations. Indeed, in the two other chordate clades, the tunicates and the cephalochordates, no such mesoderm regions are formed during development. However, in ascidians, the most studied tunicate group, some cells in the larva which participate to siphon muscles and heart formation are thought to be homologous to the cardiopharyngeal field of vertebrates. Moreover, in the cephalochordate amphioxus, lateral plate and pharyngeal mesoderm marker genes were shown to be expressed in different regions of the fully segmented paraxial mesoderm. In this work, we decided to look at the embryonic expression in amphioxus of several of these mesoderm marker genes, that could give new insights into the putative homology between cephalochordate somite regions and vertebrates’ mesoderm compartments. Here, we describe the expression pattern of Erg/Fli1a, Erg/Fli1b, Lmo2, Mesp, Npas4/4l, Osr1/2a, Osr1/2b, Tcf21/Msc and Tcf21/Mscb. Our results highlight the presence of a putative hematopoietic field in the first somite pair as previously proposed, and suggest that some genes were probably specifically recruited during vertebrate evolution for the development of pharyngeal or lateral plate mesoderm derivatives.

脊椎动物的侧板中胚层与其他中胚层相邻，在胚胎发育过程中发育成各种组织和器官，如血液、心脏、脉管系统、肾脏和平滑肌。这个中胚层室，以及产生头部肌肉和部分心脏的咽中胚层，被认为是脊椎动物的创新。事实上，在其他两种脊索动物分支中，被囊动物和头脊索动物，在发育过程中没有形成这样的中胚层区域。然而，在被研究最多的被囊动物海鞘中，一些参与鳃弓和心脏形成的幼虫细胞被认为是与脊椎动物的心咽区同源的。此外，在头脊索文昌鱼中，侧板和咽中胚层标记基因在完全分节的近轴中胚层的不同区域表达。在这项工作中，我们决定观察文昌鱼胚胎中这些中胚层标记基因的表达，这可能会为假定的头脊索虫体区域与脊椎动物中胚层隔室之间的同源性提供新的见解。我们描述了Erg/Fli1a、Erg/Fli1b、Lmo2、Mesp、npas4 / 41、Osr1/2a、Osr1/2b、Tcf21/Msc和Tcf21/Mscb的表达模式。我们的研究结果强调了先前提出的第一对体中存在一个假定的造血场，并表明一些基因可能是在脊椎动物进化过程中为咽或侧板中胚层衍生物的发育而专门招募的。

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引用次数: 0

The significance of MDK growth factor in the antler development of sika deer (Cervus nippon): An in-depth analysis MDK生长因子在梅花鹿鹿角发育中的意义：深入分析。

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2025-06-01 DOI: 10.1016/j.gep.2024.119388

Haihua Xing , Qianghui Wang , Yukai Ma, Ruobing Han, Heping Li

Deer antlers exhibit rapid growth during the velvet phase. As a critical endogenous growth factor in animals, midkine (MDK) is likely closely associated with the growth of antlers. However, the spatio-temporal expression pattern of MDK during the velvet phase was unclear. This study explored the physiological role of MDK by analyzing its molecular characterization and spatio-temporal expression dynamics during the growth of sika deer antlers. The study cloned the coding sequences (CDS) of MDK, which spanned 429 bp and encoded 142 amino acids. The results of bioinformatics prediction analysis showed that MDK was an extracellular hydrophilic secreted protein, which was mainly composed of random coil. MDK protein was relatively conserved in evolution and MDK protein of sika deer had the closest relatives to ruminants and the furthest relatives to Aves. The tip tissues (dermis, mesenchyme, precartilage, cartilage) of antlers were collected from three important growth and development nodes (early period, EP. middle period, MP. late period, LP), and quantitative real-time polymerase chain reaction (qRT-PCR) was chosen to detect the spatio-temporal expression of the MDK. The results showed that MDK was expressed in all tissue sites of antler tip in EP, MP, LP. MDK had a consistent expression pattern under all growth periods and was strongly expressed in dermis and cartilage. The expression of MDK was consistently up-regulated in precartilage, whereas it was first up-regulated and then down-regulated in other tissues, and it was highly significant in MP compared to EP and LP (P < 0.01). This study suggested that MDK may regulate the growth of dermis and cartilage tissues mainly by participating in the process of angiogenesis and bone formation, thus promoting the rapid growth of antlers.

鹿茸期鹿角生长迅速。midkine （MDK）作为一种重要的动物内源性生长因子，可能与鹿角的生长密切相关。然而，MDK在丝绒期的时空表达模式尚不清楚。本研究通过分析MDK在梅花鹿鹿角生长过程中的分子特征和时空表达动态，探讨MDK在梅花鹿鹿角生长过程中的生理作用。该研究克隆了MDK的编码序列（CDS），全长429 bp，编码142个氨基酸。生物信息学预测分析结果表明，MDK是一种细胞外亲水分泌蛋白，主要由随机线圈组成。MDK蛋白在进化中相对保守，梅花鹿MDK蛋白与反刍动物亲缘关系最近，与鸟类亲缘关系最远。从鹿角早期、EP、EP三个重要生长发育节点采集鹿角尖端组织（真皮、间质、软骨）。中期，MP。采用实时定量聚合酶链反应（qRT-PCR）检测MDK的时空表达。结果表明，MDK在鹿角尖EP、MP、LP的所有组织位点均有表达。MDK在各生长时期表达模式一致，在真皮和软骨中表达强烈。MDK的表达在不稳定状态下持续上调，而在其他组织中呈先上调后下调的趋势，且与EP和LP相比，MDK在MP中的表达非常显著(P

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引用次数: 0

Expression profile of Chchd10 gene during testicular development Chchd10基因在睾丸发育过程中的表达谱。

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2025-05-12 DOI: 10.1016/j.gep.2025.119395

Xiao Jiang , Shengqi Sun , Fei Han , Lei Sun , Jing Gu , Jia Cao , Jinyi Liu

Chchd10 protein is crucial for sustaining mitochondrial dynamics, physiology and functions, and has been reported to be most abundantly in myocardial cells and skeletal muscle. However, nothing is known for the expression pattern of Chchd10 in gonadal development. Here, we characterized the expression patterns of Chchd10 gene during embryonic gonad development and postnatal testis development in mice, as well as the expression pattern of Chchd10 gene in human puberty testis and young adult testis using publicly available datasets. Besides, we investigated the expression and distribution of Chchd10 in mice testis by RT-qPCR and immunofluorescence and analyzed the possible role and mechanism of Chchd10 in the testis. We noticed that Chchd10 showed abundant expression in embryonic testis compared to ovaries and dynamically expressed during embryonic and postnatal testis development in mice. In addition, Chchd10 was highly abundant within testicular Sertoli cells populations both in embryonic and postnatal mice and mainly located in the mitochondria of Sertoli cells in mice. Furthermore, CHCHD10 was not only enriched in Sertoli cells, but also highly expressed in tMΦ of human puberty testis and adult testis. CHCHD10 may participate in testicular development by regulating multiple biological processes of Sertoli cells. Taken together, our data indicated that Chchd10 appears to be important during testicular development, particularly in the functional modulation of Sertoli cells. Our study revealed the expression profile of Chchd10 gene during testicular development for the first time and will provide new ideas for further studying the function and molecular mechanism of Chchd10.

Chchd10蛋白对维持线粒体动力学、生理和功能至关重要，据报道在心肌细胞和骨骼肌中含量最多。然而，Chchd10在性腺发育中的表达模式尚不清楚。在这里，我们利用公开的数据集描述了Chchd10基因在小鼠胚胎性腺发育和出生后睾丸发育中的表达模式，以及Chchd10基因在人类青春期睾丸和青年成年睾丸中的表达模式。此外，我们利用RT-qPCR和免疫荧光技术研究了Chchd10在小鼠睾丸中的表达和分布，并分析了Chchd10在睾丸中的可能作用和机制。我们注意到Chchd10在胚胎睾丸中比在卵巢中表达丰富，并在小鼠胚胎和出生后睾丸发育过程中动态表达。此外，Chchd10在胚胎和出生后小鼠睾丸支持细胞群体中含量都很高，并且主要位于小鼠支持细胞的线粒体中。此外，CHCHD10不仅在人青春期睾丸和成人睾丸的支持细胞中富集，而且在tMΦ中也有高表达。CHCHD10可能通过调节支持细胞的多种生物学过程参与睾丸发育。综上所述，我们的数据表明Chchd10似乎在睾丸发育过程中很重要，特别是在支持细胞的功能调节中。本研究首次揭示了Chchd10基因在睾丸发育过程中的表达谱，将为进一步研究Chchd10的功能和分子机制提供新的思路。

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引用次数: 0

Transcriptomics and phenotypic analysis of OTOF gene knockdown in zebrafish mediated by CRISPR/Cas9 CRISPR/Cas9介导的斑马鱼OTOF基因敲低的转录组学和表型分析

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2025-04-16 DOI: 10.1016/j.gep.2025.119394

Xuejing Bai, Wenbo Xu, Ying Zhu, Beibei Luo, Dan Ye

Deafness is a common genetic disorder, where mutations,in the OTOF gene can disrupt the normal functionof the Otoferlin protein, leading to impaired neurotransmitter release in the inner ear and subsequent deafness. Despite the complexity of the pathogenic mechanism,it is not fully understood. Zebrafish are an excellent model for studying genetically-induced deafness,but there have been no previous reports on the pathogenesis of OTOF in zebrafish.This study successfully established a zebrafish model with mutated OTOF genes using CRISPR/Cas9 gene editing technology to investigate the molecular basis of OTOF-induced deafness. Compared to AB wild type zebrafish, those with low otof expression showed injury and apoptosis of hair cells in the posterior lateral neuromasts along with significant increase in the number of macrophages and apoptotic cells in this region. Additionally, these mutants exhibited a reduction in body length. To further elucidate differences at 5dpf (days post-fertilization) between mutant and wild type zebrafish embryos, RNA-seq analysis was conducted to examine differentially expressed genes (DEGs).A total of 334 up-regulated DEGs and 111 down-regulated DEGs were identified in mutants compared to wild types.KEGG and GO enrichment analyses were performed on these DEGs to identify key signaling pathways and hub DEGs. The findings revealedan increased expression of several genes involved in the HSP70 oxidative stress system, suggesting that OTOF may protect cochlear hair cell from apoptosis induced by oxidative stress through regulation of MAPK signal and HSP70 expression.In summary, the establishment of a zebrafish model with OTOF knockout provides a valuable tool for investigating the function of Otoferlin and understanding the role of the OTOF gene in deafness. These potential molecular insights offer significant contributions towards understanding the pathogenesis of deafness experimental models and serves as a foundation for comprehending the involvement of the OTOF gene.

耳聋是一种常见的遗传性疾病，其中OTOF基因的突变可以破坏Otoferlin蛋白的正常功能，导致内耳神经递质释放受损，从而导致耳聋。尽管致病机制复杂，但尚未完全了解。斑马鱼是研究遗传性耳聋的良好模型，但目前还没有关于斑马鱼耳聋发病机制的报道。本研究利用CRISPR/Cas9基因编辑技术成功建立了OTOF基因突变的斑马鱼模型，探讨OTOF致耳聋的分子基础。与AB野生型斑马鱼相比，低表达斑马鱼后外侧神经突毛细胞损伤和凋亡，该区域巨噬细胞和凋亡细胞数量显著增加。此外，这些突变体表现出体长的减少。为了进一步阐明突变型和野生型斑马鱼胚胎在5dpf（受精后天数）的差异，采用RNA-seq分析来检测差异表达基因（DEGs）。与野生型相比，突变体中共鉴定出334个上调的deg和111个下调的deg。对这些deg进行了KEGG和GO富集分析，以确定关键信号通路和枢纽deg。研究结果显示，HSP70氧化应激系统中多个相关基因的表达增加，提示otoof可能通过调控MAPK信号和HSP70的表达，保护耳蜗毛细胞免受氧化应激诱导的凋亡。综上所述，建立OTOF基因敲除的斑马鱼模型，为研究Otoferlin的功能和了解OTOF基因在耳聋中的作用提供了有价值的工具。这些潜在的分子见解为理解耳聋的发病机制提供了重要的贡献，并为理解OTOF基因的参与奠定了基础。

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引用次数: 0

Lymphangiogenesis in mouse embryonic and early postnatal ovaries 小鼠胚胎和出生后早期卵巢的淋巴管生成。

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2025-04-05 DOI: 10.1016/j.gep.2025.119393

Hongyu Su , Zhilin Zhou , Fan Ye , Hang Xiong , Ziheng Huang , Jiayi Shen , Tao Yi , Hongying Zhou

Background

The critical importance and remodeling capacity of the blood vasculature within the ovary have been extensively analyzed, while the lymphatic vasculature has received limited attention, and its characteristics were reported by only a few studies.

Objectives

To investigate the morphological patterns of lymphatic vessel formation in mouse embryonic and early postnatal ovary.

Material and methods

Immunostaining was performed on ovarian tissues from Prox1-EGFP transgenic mice and wild-type mice to investigate the lymphangiogenesis, and the CD31 was employed to label vascular endothelium and LYVE1 and Prox1 as co-markers for lymphatic endothelium.

Result

Prox1⁺ cells, LYVE1⁺ cells, and Prox1-EGFP⁺/LYVE1⁺ vessels were absent in the medulla near the ovarian hilum until P7, and YAP1 was expressed in the nucleus and cytoplasm of granulosa cells, alongside the observation of scattered primary follicles within the ovary. By P10, these markers were abundantly expressed in the ovarian medulla, while YAP1 was localized primarily in the cytoplasm of granulosa cells, alongside the occurrence of secondary follicles. By P14, Prox1⁺ cells, LYVE1⁺ cells, and Prox1-EGFP⁺/LYVE1⁺ vessels extended to the cortex, and YAP1 shifted to the nuclei of granulosa cells, alongside the reaching of follicles to the antral stage. From P14 to P28, these markers exhibited a further increasing trend, while YAP1 localized in the nucleus of granulosa cells, alongside the progress of follicles to the preovulatory stage.

Conclusion

Ovarian lymphangiogenesis in the mouse began on postnatal day 7 and subsequently developed from the medulla to the cortex. Additionally, ovarian lymphangiogenesis occurred in synchrony with follicular maturation, during which YAP1 exhibited an ectopic expression pattern.

背景：卵巢内血管的重要性和重塑能力已经得到了广泛的分析，而淋巴血管受到的关注有限，其特征的报道也很少。目的：探讨小鼠胚胎期和产后早期卵巢淋巴管形成的形态学模式。材料与方法：采用免疫染色法观察Prox1- egfp转基因小鼠和野生型小鼠卵巢组织的淋巴管生成情况，CD31标记血管内皮，LYVE1和Prox1作为淋巴内皮的共标记物。结果：卵巢门附近髓质至P7无Prox1+细胞、LYVE1+细胞和Prox1- egfp +/LYVE1+血管，颗粒细胞细胞核和细胞质中有YAP1表达，卵巢内可见散在的初代卵泡。P10时，这些标记物在卵巢髓质中大量表达，主要分布在颗粒细胞的细胞质中，伴随着继发卵泡的出现。到P14期，Prox1+细胞、LYVE1+细胞和Prox1- egfp +/LYVE1+血管延伸到皮层，YAP1转移到颗粒细胞的细胞核，同时到达卵泡至正中期。从P14到P28，随着卵泡进入排卵期，这些标记物呈进一步增加的趋势。结论：小鼠卵巢淋巴管生成始于出生后第7天，随后由髓质向皮层发育。此外，卵巢淋巴管生成与卵泡成熟同步发生，在此过程中YAP1表现出异位表达模式。

{"title":"Lymphangiogenesis in mouse embryonic and early postnatal ovaries","authors":"Hongyu Su , Zhilin Zhou , Fan Ye , Hang Xiong , Ziheng Huang , Jiayi Shen , Tao Yi , Hongying Zhou","doi":"10.1016/j.gep.2025.119393","DOIUrl":"10.1016/j.gep.2025.119393","url":null,"abstract":"<div><h3>Background</h3><div>The critical importance and remodeling capacity of the blood vasculature within the ovary have been extensively analyzed, while the lymphatic vasculature has received limited attention, and its characteristics were reported by only a few studies.</div></div><div><h3>Objectives</h3><div>To investigate the morphological patterns of lymphatic vessel formation in mouse embryonic and early postnatal ovary.</div></div><div><h3>Material and methods</h3><div>Immunostaining was performed on ovarian tissues from Prox1-EGFP transgenic mice and wild-type mice to investigate the lymphangiogenesis, and the CD31 was employed to label vascular endothelium and LYVE1 and Prox1 as co-markers for lymphatic endothelium.</div></div><div><h3>Result</h3><div>Prox1<sup>+</sup> cells, LYVE1<sup>+</sup> cells, and Prox1-EGFP<sup>+</sup>/LYVE1<sup>+</sup> vessels were absent in the medulla near the ovarian hilum until P7, and YAP1 was expressed in the nucleus and cytoplasm of granulosa cells, alongside the observation of scattered primary follicles within the ovary. By P10, these markers were abundantly expressed in the ovarian medulla, while YAP1 was localized primarily in the cytoplasm of granulosa cells, alongside the occurrence of secondary follicles. By P14, Prox1<sup>+</sup> cells, LYVE1<sup>+</sup> cells, and Prox1-EGFP<sup>+</sup>/LYVE1<sup>+</sup> vessels extended to the cortex, and YAP1 shifted to the nuclei of granulosa cells, alongside the reaching of follicles to the antral stage. From P14 to P28, these markers exhibited a further increasing trend, while YAP1 localized in the nucleus of granulosa cells, alongside the progress of follicles to the preovulatory stage.</div></div><div><h3>Conclusion</h3><div>Ovarian lymphangiogenesis in the mouse began on postnatal day 7 and subsequently developed from the medulla to the cortex. Additionally, ovarian lymphangiogenesis occurred in synchrony with follicular maturation, during which YAP1 exhibited an ectopic expression pattern.</div></div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"55 ","pages":"Article 119393"},"PeriodicalIF":1.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Hippo signaling components in the early dorsal pancreatic bud 早期胰腺背芽中Hippo信号成分的表征。

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2025-03-11 DOI: 10.1016/j.gep.2025.119392

Neha Ahuja , Caitlin Maynard , Tyler Bierschenck , Ondine Cleaver

All pancreatic lineages originate from a transitory structure known as the multipotent progenitor epithelium (MPE), which is an endodermal placode formed via epithelial stratification. Cells within the MPE undergo de novo lumenogenesis to give rise to an epithelial plexus, which serves as a progenitor niche for subsequent development of endocrine, ductal and acinar cell types. Recent evidence suggests that Hippo signaling is required for pancreatic cell differentiation, but little is known about the function of Hippo signaling in the development of the MPE. Here, we characterize the expression of YAP1, TAZ, and the Hippo regulators LATS1/2 kinases and MERLIN in early murine pancreatic epithelium, during epithelial stratification, plexus development and emergence of endocrine cells. We find that YAP1 expression is relatively low in the pancreas bud during stratification but increases by E11.5. Intriguingly, we find differing patterns of TAZ and YAP1 immunoreactivty throughout pancreatic development. We further find that MERLIN and LATS1/2 kinases are expressed during the period of rapid stratification and become markedly apical at nascent lumens. To gain a better understanding of how Hippo signaling and lumen formation are connected, we analyzed the subcellular localization of Hippo signaling components during varying stages of lumen formation and found that they are dynamically localized during lumenogenesis. Together, our results point to a previously unsuspected relationship between Hippo signaling and lumen formation during pancreatic development.

所有胰腺谱系都起源于一种称为多能祖上皮（MPE）的暂时性结构，这是一种通过上皮分层形成的基板。MPE内的细胞经过新生的放光形成，形成上皮丛，作为内分泌、导管和腺泡细胞类型后续发育的祖生态位。最近的证据表明，Hippo信号是胰腺细胞分化所必需的，但对Hippo信号在MPE发育中的功能知之甚少。在这里，我们表征了YAP1、TAZ、Hippo调节因子LATS1/2激酶和MERLIN在早期小鼠胰腺上皮、上皮分层、神经丛发育和内分泌细胞出现期间的表达。我们发现YAP1在胰腺芽分层期间表达相对较低，但在E11.5时表达增加。有趣的是，我们发现TAZ而不是YAP1在早期内分泌细胞中表达。我们进一步发现，MERLIN和LATS1/2激酶在快速分层期间强烈表达，并在新生管腔中明显变为顶端。为了更好地了解Hippo信号与管腔形成之间的联系，我们分析了在管腔形成的不同阶段Hippo信号成分的表达，发现它们在管腔形成过程中是动态定位的。总之，我们的研究结果指出了胰腺发育过程中Hippo信号传导与管腔形成之间的关系。

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引用次数: 0

Pre-gestational restraint stress affects reproductive outcomes in adult rats by modulating ovarian and uterine function 妊娠前约束应激通过调节卵巢和子宫功能影响成年大鼠的生殖结局

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2025-02-25 DOI: 10.1016/j.gep.2025.119391

Harini Raghavendhira , Divya Srinivasan , Jeyakumari Paul , Ravindran Rajan , Ravi Sankar Bhaskaran

The impact of gestational stress on reproductive outcomes is well-established, but the effects of pre-gestational stress remain inconclusive. Using female Wistar rats, we demonstrated that pre-gestational stress negatively affects fertility and pregnancy outcomes. The rats were subjected to restraint stress (RS) for 15 days, with 3 h of stress each day, before mating. The RS group exhibited higher levels of corticosterone and prolactin, along with lower levels of adrenocorticotropic hormone (ACTH), indicating a successful stress model. Stressed rats showed reduced fertility and fecundity indices, longer conception times, and decreased levels of ovarian steroids, such as progesterone, testosterone, and estradiol. Additionally, the ovaries of the RS group had fewer antral follicles and more ovarian cysts. Elevated protein levels of cytochrome P450 side-chain cleavage enzyme (CYP11A1) and aromatase (CYP19A1), along with decreased levels of 17β-hydroxysteroid dehydrogenase (17β-HSD), indicating impaired ovarian steroidogenesis in stress exposed rats. In the RS group, there was a significant increase in proteins associated with folliculogenesis, specifically octamer-binding transcription factor 4 (OCT 4) and growth differentiation factor 9 (GDF 9). Additionally, proteins linked to ovulation, such as the prolactin receptor (PRLR), peroxisome proliferator-activated receptor-γ (PPAR-γ), and cyclooxygenase 2 (COX 2), were elevated. The increased levels of PRLR, progesterone receptor (PR), androgen receptor (AR), and estrogen receptor (ER) combined with heightened oxidative stress in the uteri of the RS group, suggest a potential disruption in uterine function. Overall, this research indicates that pre-gestational stress can significantly impact reproductive health by altering gonadotrophin and ovarian steroid dynamics in the female reproductive tract.

妊娠应激对生殖结果的影响是公认的，但妊娠前应激的影响仍不确定。使用雌性Wistar大鼠，我们证明了妊娠前应激对生育能力和妊娠结局有负面影响。交配前给大鼠施加限制性应激（RS） 15 d，每天应激3 h。RS组表现出较高水平的皮质酮和催乳素，以及较低水平的促肾上腺皮质激素（ACTH），表明应激模型成功。应激大鼠的生育能力和繁殖力指数下降，受胎时间延长，卵巢类固醇激素（如黄体酮、睾酮和雌二醇）水平下降。此外，RS组卵巢有更少的窦卵泡和更多的卵巢囊肿。细胞色素P450侧链切割酶（CYP11A1）和芳香化酶（CYP19A1）蛋白水平升高，17β-羟基类固醇脱氢酶（17β-HSD）水平降低，表明应激暴露大鼠卵巢类固醇生成受损。在RS组中，与卵泡发生相关的蛋白质显著增加，特别是八聚体结合转录因子4 （OCT 4）和生长分化因子9 （GDF 9）。此外，与排卵相关的蛋白质，如催乳素受体（PRLR）、过氧化物酶体增殖物激活受体-γ (PPAR-γ)和环氧化酶2 （COX 2）均升高。RS组子宫内PRLR、孕酮受体（PR）、雄激素受体（AR）和雌激素受体（ER）水平升高，并伴有氧化应激升高，提示子宫功能可能受到破坏。总体而言，本研究表明，妊娠前应激可通过改变女性生殖道的促性腺激素和卵巢类固醇动态而显著影响生殖健康。

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引用次数: 0

Transcriptome analysis of Berberine induced accelerated tail fin regeneration in Zebrafish larvae 小檗碱诱导斑马鱼幼鱼尾鳍加速再生的转录组分析

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2025-02-09 DOI: 10.1016/j.gep.2025.119390

Radhika Gupta , Chitra Bhasin , Adita Joshi , Nisheeth Agarwal , Ayush Aggarwal , Padmshree Mudgal

Humans have limited capacity to regenerate lost tissues post injury. The ability to modulate regenerative repair of tissues offers possibilities for restoring loss of tissue (organ) structure and function. Zebrafish (Danio rerio) larvae fin fold regeneration model is a simple system to study the process of regeneration and associated cellular mechanisms. Berberine, a plant alkaloid which is known to have wound healing properties shows potential to modulate regeneration. The present study aimed to explore the modulating influence of berberine on the signaling pathways involved in zebrafish larvae transected tail fin fold regeneration. Tail fin fold transection was performed on 3 dpf (days post fertilization) zebrafish larvae treated with Berberine (0.01%) and untreated control (System water (SW)). The larvae were observed under a microscope at 0, 1, 2, 3, 4, 5, hours post transection (hpt). RNA was extracted from Berberine treated and untreated (control) tail fin transected larvae at 4 hpt to perform RNA-seq analysis. PPI (protein-protein interaction) network, Shiny GO functional enrichment and topology analysis of DEGs (differentially expressed genes) was performed. Berberine treated larvae showed an accelerated regeneration growth in their transected tail fin by 4 hpt. Berberine induced accelerated regeneration is associated with the involvement of Insulin, IGF, stress response, jak-stat, cytokine, and cellular reprogramming signaling pathways as per RNA-seq analysis and String PPI network, and Shiny GO functional enrichment analysis of DEGs. Topological analysis using Cytohubba revealed tnfa, stat3, jak2b, igf1, jak1, hsp90aa1.1, stat1a, stat1b, bag3, hsp70, and fosl1a as the key Hub genes in the PPI network. The present study identifies the pathways and the Hub proteins involved in berberine induced accelerated regeneration process in zebrafish larvae.

人类在受伤后再生失去组织的能力有限。调节组织再生修复的能力为恢复组织（器官）结构和功能的损失提供了可能性。斑马鱼（Danio rerio）鱼鳍再生模型是研究鱼鳍再生过程和相关细胞机制的简单系统。小檗碱，一种已知具有伤口愈合特性的植物生物碱，显示出调节再生的潜力。本研究旨在探讨黄连素对斑马鱼幼体横断尾鳍再生信号通路的调节作用。对3 dpf（受精后d）斑马鱼仔鱼进行尾鳍横断，分别用0.01%的小檗碱处理和未处理的对照（系统水（SW））。分别于横切后0、1、2、3、4、5小时在显微镜下观察。从4 hpt时经小檗碱处理和未处理的（对照）尾鳍横断的幼虫中提取RNA，进行RNA-seq分析。进行了蛋白-蛋白相互作用（PPI）网络、差异表达基因（DEGs）的Shiny GO功能富集和拓扑分析。经小檗碱处理后，其横切尾鳍的再生生长速度加快了4 hpt。根据RNA-seq分析、String PPI网络和DEGs的Shiny GO功能富集分析，小檗碱诱导的加速再生与胰岛素、IGF、应激反应、jak-stat、细胞因子和细胞重编程信号通路的参与有关。利用Cytohubba进行拓扑分析，发现tnfa、stat3、jak2b、igf1、jak1、hsp90aa1.1、stat1a、stat1b、bag3、hsp70和fosl1a是PPI网络中的关键枢纽基因。本研究确定了小檗碱诱导斑马鱼幼体加速再生过程的途径和Hub蛋白。

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引用次数: 0

Sept10 and sept12 are expressed in specific proliferating cells in zebrafish brain Sept10和sept12在斑马鱼大脑中特异性增殖细胞中表达。

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2024-12-12 DOI: 10.1016/j.gep.2024.119387

Constantin Berger , Julia Katharina Charlotte Kreß , Frederik Helmprobst

Septins are a group of cytoskeletal GTP binding proteins which are involved in different cellular processes, like cell division, exocytosis and axon growth. Their function, especially in the nervous system, is not clear. In zebrafish 16 different septins are described and for some of them the expression in the brain is described. Interestingly, the expression pattern of several of them is highly specific. Here we describe the expression of sept10 and sept12 in the developing zebrafish brain and found that these show a very defined expression pattern. Interestingly, they show an overlap with a group, but not all proliferating PCNA positive cells in nervous tissue.

赛普特蛋白是一组细胞骨架 GTP 结合蛋白，参与细胞分裂、外吞和轴突生长等不同的细胞过程。它们的功能，尤其是在神经系统中的功能尚不清楚。斑马鱼体内有 16 种不同的隔膜蛋白，其中一些在大脑中的表达也有描述。有趣的是，其中几种的表达模式具有高度特异性。在这里，我们描述了 sept10 和 sept12 在发育中的斑马鱼大脑中的表达，并发现它们表现出非常明确的表达模式。有趣的是，它们与神经组织中的一组 PCNA 阳性细胞有重叠，但不是所有增殖的 PCNA 阳性细胞。

引用次数: 0

Spatial and temporal expression analysis of BMP signal modifiers, Smoc1 and Smoc2, from postnatal to adult developmental stages in the mouse testis 小鼠睾丸从出生后到成年发育阶段BMP信号修饰因子Smoc1和Smoc2的时空表达分析。

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns

Pub Date : 2024-12-01 DOI: 10.1016/j.gep.2024.119383

Michio Ono , Kuniko Nakajima , Shin-ichi Tomizawa , Takayuki Shirakawa , Ippei Okada , Hirotomo Saitsu , Naomichi Matsumoto , Kazuyuki Ohbo

Smoc1 and Smoc2, members of the SPARC family of genes, encode signaling molecules downstream of growth factors such as the TGF-β, FGF, and PDGF families. Smoc1 has been implicated in playing a crucial role in microphthalmia with limb anomalies in humans and mice, while Smoc2 deficiency causes dental developmental defects. Although developmental cytokines/growth factors including TGF-β superfamily have been shown to play critical roles in postnatal spermatogenesis, there are no reports analyzing the spatial and temporal expression of Smoc1 and Smoc2 in the postnatal testis. In this study, we investigated the mRNA and protein expression of Smoc1 and Smoc2 in neonatal, juvenile, and adult mouse testes by RNA in situ hybridization, immunofluorescence, and single-cell RNA-seq analysis. We show that Smoc1 and Smoc2 have distinct expression patterns in male germ cells: Smoc1 is more highly expressed than Smoc2 in the germline. In contrast, Smoc2 is highly expressed in testicular somatic cells from neonatal to juvenile stages. The Smoc2-expressing cells then switch from somatic cells to germ cells in adults. Thus, although SMOC1 and SMOC2 proteins are structurally very similar, their spatial and temporal expression patterns in the postnatal testis differ significantly, suggesting their distinct roles in reproduction.

Smoc1和Smoc2是SPARC家族基因的成员，编码生长因子（如TGF-β、FGF和PDGF家族）下游的信号分子。Smoc1 被认为在人类和小鼠的小眼症和肢体异常中起着关键作用，而 Smoc2 的缺乏则会导致牙齿发育缺陷。虽然包括 TGF-β 超家族在内的发育细胞因子/生长因子已被证明在产后精子发生中发挥关键作用，但目前还没有报告分析 Smoc1 和 Smoc2 在产后睾丸中的空间和时间表达。本研究通过RNA原位杂交、免疫荧光和单细胞RNA-seq分析，研究了Smoc1和Smoc2在新生小鼠、幼鼠和成年小鼠睾丸中的mRNA和蛋白表达。我们发现，Smoc1和Smoc2在雄性生殖细胞中有不同的表达模式：在生殖细胞中，Smoc1的表达量比Smoc2高。相比之下，Smoc2在睾丸体细胞中的表达量从新生儿到幼年阶段都很高。表达 Smoc2 的细胞在成年后会从体细胞转变为生殖细胞。因此，尽管SMOC1和SMOC2蛋白在结构上非常相似，但它们在出生后睾丸中的空间和时间表达模式却大不相同，这表明它们在生殖过程中发挥着不同的作用。

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