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Effect of bacterial diversity on the quality of fermented apple juice during natural fermentation of Hanfu apples 汉富苹果自然发酵过程中细菌多样性对发酵苹果汁质量的影响
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-29 DOI: 10.1007/s10068-024-01593-1
Fangping Wang, Danshi Zhu, Doudou Wu, Yueyi Zhang, Minhui Yang, Xuehui Cao, He Liu

The fermented cloudy apple juice (FCAJ) bacterial phase was analyzed by 16S rRNA gene-based sequencing. During fermentation, the bacterial phase transition promoted quality changes, such as carbohydrate, organic acid, total phenol, taste and volatiles of FCAJ. Citrobacter and Lactobacillus were the dominant bacterial genera of Hanfu apple juice by natural fermentation, and lactic acid was the most abundant organic acid in FCAJ. Citrobacter showed a continuous increase trend along with fermentation time, while Lactobacillus showed a slight decrease during the later period of fermentation. The contents of total phenolic and flavonoid both showed a trend of rising first and then decreasing in FCAJ during fermentation. Alcohols and esters, the main aromatic volatiles in FCAJ, showed significant increases, especially for ethanol, 3-methyl-1-butanol and ethyl acetate. Citrobacter presented a higher correlation than Lactobacillus with some volatile flavor.

通过 16S rRNA 基因测序分析了发酵浑浊苹果汁(FCAJ)的菌相。在发酵过程中,菌相转变促进了 FCAJ 的质量变化,如碳水化合物、有机酸、总酚、味道和挥发性物质。柠檬酸杆菌和乳酸杆菌是汉富苹果汁自然发酵的优势菌属,乳酸是汉富苹果汁中含量最高的有机酸。柠檬酸杆菌随着发酵时间的延长呈持续上升趋势,而乳酸杆菌在发酵后期略有下降。总酚和类黄酮的含量在 FCAJ 发酵过程中均呈先上升后下降的趋势。酒精和酯类是 FCAJ 中主要的芳香挥发物,其含量显著增加,尤其是乙醇、3-甲基-1-丁醇和乙酸乙酯。柠檬酸杆菌与某些挥发性味道的相关性高于乳酸杆菌。
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引用次数: 0
Improvement of sleep disorders through the adenosine A receptor agonist effect of Phlomoides umbrosa Turczaninow root extract in pentobarbital-induced ICR mice 通过伞形科植物根提取物的腺苷 A 受体激动剂作用改善戊巴比妥诱导的 ICR 小鼠的睡眠障碍
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-28 DOI: 10.1007/s10068-024-01663-4
Joo-Hyun Oh, Yoon-Young Han, Eun-Bi Kim, Ha-Neul Jo, Jae-Sun Lee, Bo-Mi Kim, Ji-Min Kim, Young-Seob Lee, Dae Young Lee, Kwan-Woo Kim, Inil Lee, Yong-Wook Lee, Chan-Sung Park, Dae-Ok Kim

Sleep deprivation is a serious problem that deteriorates the health, quality of life, and social productivity of affected individuals. Research on safe and effective plant-based materials should be performed to help alleviate sleep disorders. Phlomoides umbrosa Turczaninow is a domestic plant in South Korea that has been used traditionally as a medicine to treat sleep disorders. The effects and mechanism of action of a P. umbrosa Turczaninow root extract (PUTRE) prepared using hot water extraction were investigated. ICR mice were injected with pentobarbital to induce sleep. The PUTRE-treated groups had significantly (p < 0.05) decreased sleep latency and increased sleep time compared with the controls. When PUTRE was co-administered with adenosine antagonists (caffeine and 8-cyclopentyl-1,3-dipropylxanthine), it acted specifically on the adenosine A1 receptor, because its sleep-enhancing effect was counteracted by the adenosine antagonist. This is the first study to demonstrate that PUTRE can induce sleep through adenosine A1 receptor-specific agonist activity. Therefore, PUTRE is suggested as a valuable sleep-enhancing substance.

睡眠不足是一个严重的问题,它会恶化受影响者的健康、生活质量和社会生产力。应该对安全有效的植物材料进行研究,以帮助缓解睡眠障碍。Phlomoides umbrosa Turczaninow 是韩国的一种家生植物,传统上一直被用作治疗睡眠障碍的药物。本研究调查了用热水提取法制备的鳞脐草根提取物(PUTRE)的效果和作用机制。给 ICR 小鼠注射戊巴比妥诱导睡眠。与对照组相比,PUTRE 治疗组的睡眠潜伏期明显缩短(p < 0.05),睡眠时间明显延长。当PUTRE与腺苷拮抗剂(咖啡因和8-环戊基-1,3-二丙基黄嘌呤)同时服用时,它专门作用于腺苷A1受体,因为其促进睡眠的作用被腺苷拮抗剂抵消了。这是首次证明 PUTRE 可通过腺苷 A1 受体特异性激动剂活性诱导睡眠的研究。因此,PUTRE 被认为是一种有价值的睡眠促进物质。
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引用次数: 0
Plant-based probiotic foods: current state and future trends 植物性益生菌食品:现状与未来趋势
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-28 DOI: 10.1007/s10068-024-01674-1
Alan Portal D’Almeida, Aida Aguilera Infante Neta, Micael de Andrade-Lima, Tiago Lima de Albuquerque

Plant-based probiotic foods (PBPFs) have recently become a notable choice for many consumers. While less recognized than dairy products, these foods offer efficient alternatives for individuals with lactose intolerance, vegans, or those aiming for more sustainable dietary practices. Traditional fermented PBPFs, such as kimchi, sauerkraut, and kombucha, are part of cultures from different countries and have gained more significant popularity in recent years globally due to their peculiar flavors and health benefits. However, new plant-based probiotic products have also been studied and made available to consumers of the growing demand in this sector. Therefore, this review discusses trends in plant-based probiotic production, known benefits, and characteristics. Challenges currently faced in manufacturing, distribution, marketing, consumer acceptance, and legislation are also discussed.

摘要植物性益生菌食品(PBPFs)最近已成为许多消费者的显著选择。虽然与乳制品相比,这些食品的知名度较低,但它们为乳糖不耐症患者、素食者或追求更可持续饮食习惯的人提供了有效的替代品。传统的发酵 PBPF,如泡菜、酸菜和昆布茶,是不同国家文化的一部分,由于其独特的风味和对健康的益处,近年来在全球越来越受欢迎。然而,人们也在研究新的植物性益生菌产品,并将其提供给需求不断增长的消费者。因此,本综述将讨论植物性益生菌的生产趋势、已知益处和特点。此外,还讨论了目前在生产、分销、营销、消费者接受度和立法方面面临的挑战。
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引用次数: 0
A review on preparation and application of low-calorie structured lipids in food system 低热量结构脂在食品系统中的制备和应用综述
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-26 DOI: 10.1007/s10068-024-01689-8
Hira Ijaz, Shangde Sun

Low-calorie structured lipids are an advanced form of functional lipids made by changing the position of fatty acids attached to the glycerol backbone. The main reason for their production is to get nutraceutical lipids. Different methods are used to synthesize low-calorie structured lipids, like chemical or enzymatic methods. Initially, these lipids are prepared by using chemical methods. Synthesis of low-calorie structured lipids using enzymes is now in demand due to several advantages like good catalytic efficiency, environmentally friendly, and moderate reaction conditions. Enzymatic interesterification is mostly used in industries to make modified lipids like low-calorie structured lipids, human milk substitutes, cocoa butter equivalents, margarine, and shortenings. This review summarizes the synthesis, uses and clinical applications of modified lipids in food systems.

低热量结构脂是通过改变甘油骨架上脂肪酸的位置而制成的一种高级功能脂。生产这种脂质的主要原因是为了获得营养保健脂质。合成低热量结构脂的方法多种多样,如化学或酶法。最初,这些脂质是用化学方法制备的。由于具有催化效率高、环境友好、反应条件温和等优点,使用酶合成低热量结构脂类现在很受欢迎。酶法酯化主要用于工业领域,制造改性脂类,如低热量结构脂、人奶替代品、可可脂等同物、人造奶油和起酥油。本综述概述了食品系统中改性脂质的合成、用途和临床应用。
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引用次数: 0
Preparation and properties of hydrogels with different forms of nanocellulose and low methoxyl pectin 含有不同形式纳米纤维素和低甲氧基果胶的水凝胶的制备及其特性
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-23 DOI: 10.1007/s10068-024-01684-z
Xiaolan Shang, Xiaojin Geng, Huiping Lei, Jing Tan, Chunyan Xie

Different proportions of cellulose nanofibers (CNFs)/cellulose nanocrystals (CNCs) and low methoxyl (LM) pectin were used to prepare hydrogels. By analyzing the apparent morphology, gel strength, rheological characteristics, microstructure, and interaction between cellulose and LM pectin, the characteristics of hydrogels created by the combination of different forms of nanocellulose and LM pectin were compared. At the same concentration, the strength of hydrogel formed by the combination of CNCs and LM pectin was higher than hydrogel formed by the combination of CNFs and LM pectin, which was consistent with the gel structure. The gel formed by the combination of LM pectin and CNFs had stronger viscoelasticity than the gel formed by the combination of LM pectin and CNCs. When the ratio of LM pectin to CNFs/CNCs is 0.5/0.5, a better gel network structure is formed, and the viscoelastic properties of the gel formed at this concentration under shock conditions are better protected.

采用不同比例的纤维素纳米纤维(CNFs)/纤维素纳米晶体(CNCs)和低甲氧基果胶(LM)制备水凝胶。通过分析表观形态、凝胶强度、流变特性、微观结构以及纤维素和低甲氧基果胶之间的相互作用,比较了不同形式的纳米纤维素和低甲氧基果胶组合制备的水凝胶的特性。在相同浓度下,CNCs 和 LM 果胶组合形成的水凝胶强度高于 CNFs 和 LM 果胶组合形成的水凝胶,这与凝胶结构一致。LM 果胶与 CNFs 结合形成的凝胶比 LM 果胶与 CNCs 结合形成的凝胶具有更强的粘弹性。当 LM 果胶与 CNFs/CNCs 的比例为 0.5/0.5 时,可形成更好的凝胶网络结构,在此浓度下形成的凝胶在冲击条件下的粘弹性能得到更好的保护。
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引用次数: 0
Inactivation effect of extracts of gardenia fruit, licorice and Torilis japonica fruit against Bacillus spores 栀子果、甘草和榧子提取物对芽孢杆菌孢子的灭活作用
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-22 DOI: 10.1007/s10068-024-01679-w
Won Il Cho, Sang Hoon Song

For the development of natural antimicrobial agents targeting Bacillus subtilis spores, this study compared the inactivation effects of ethanol extracts from gardenia fruit, licorice, and Torilis japonica fruit against those of commercial antibacterial agents. A 0.01% (w/w) concentration of polylysine with surfactant property exhibited a sporicidal effect, reducing Bacillus spore concentrations by 2 log CFU/g. Ethanol extracts of gardenia fruit at 0.05% and Torilis japonica fruit at 0.01% concentration showed significant bactericidal effects on both Bacillus spores and vegetative cells, achieving a reduction of 2–4 log CFU/g within 2–48 h. Licorice ethanol extract at 0.005% concentration displayed in a 2–4 log CFU/g after 24–48 h coinciding with spore conversion to vegetative cells. A combination of ethanol extracts of 0.05% gardenia fruit and 0.005% licorice produced a bactericidal effect, resulting in a 2–5 log CFU/g reduction over 48 h of growth.

为了开发针对枯草杆菌孢子的天然抗菌剂,本研究比较了栀子果实、甘草和榧子的乙醇提取物与商业抗菌剂的灭活效果。浓度为 0.01%(重量比)的具有表面活性剂特性的聚赖氨酸具有杀孢作用,可将芽孢杆菌孢子浓度降低 2 log CFU/g。浓度为 0.05% 的栀子果实乙醇提取物和浓度为 0.01% 的香榧果实乙醇提取物对芽孢杆菌孢子和无性细胞都有显著的杀菌作用,在 2-48 小时内可减少 2-4 个对数 CFU/g。0.05% 的栀子果乙醇提取物和 0.005% 的甘草乙醇提取物的组合产生了杀菌效果,在 48 小时的生长过程中减少了 2-5 log CFU/g。
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引用次数: 0
Genetically modified crops and sustainable development: navigating challenges and opportunities 转基因作物与可持续发展:驾驭挑战与机遇
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-21 DOI: 10.1007/s10068-024-01669-y
Rubby Sandhu, Nischay Chaudhary, Rafeeya Shams, Kshirod Kumar Dash

The dominance of Genetically Modified (GM) crops in global agriculture is underscored by the production quantities of GM Maize and GM soybean. This review discusses the global statistics of GM crops mentioning the area of cultivation, production, and adoption rates of GM crops in detail. It relates the comprehensive overview of perception toward GM technology varying across regions, with the younger generation often displaying a preferable outlook. It highlights the environmental benefits of GM crops, explaining the decreased pesticide usage with potential health benefits, and hinting at an indirect decline in cancer occurrences. An overview of advancements in genetic tools have significantly evolved genome editing techniques in agriculture. The use of such tools in the field of agriculture shows promising effects in maintaining sustainable development goals. This review demonstrates that genetic techniques are a responsible and effective tool for generating GM crops to promote sustainable agriculture.

转基因玉米和转基因大豆的产量凸显了转基因作物在全球农业中的主导地位。本综述详细讨论了全球转基因作物的种植面积、产量和采用率等统计数据。综述全面介绍了各地区对转基因技术的不同看法,其中年轻一代往往对转基因技术持乐观态度。报告强调了转基因作物对环境的益处,解释了减少杀虫剂的使用对健康的潜在益处,并暗示癌症发病率会间接下降。遗传工具的进步极大地促进了农业基因组编辑技术的发展。这些工具在农业领域的应用为实现可持续发展目标带来了希望。本综述表明,基因技术是产生转基因作物以促进可持续农业的一种负责任的有效工具。
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引用次数: 0
Physico-chemical and microbiological properties of water kefir produced from carob (Ceratonia siliqua L.) sherbet 用角豆树(Ceratonia siliqua L.)果汁生产的水酸乳的物理化学和微生物特性
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-21 DOI: 10.1007/s10068-024-01682-1
Mehmet Fuat Gülhan, Ayca Gülhan, Cihan Düşgün

In this study, sugar-free (A) and sugar-added (B) formulations of carob sherbet were fermented with water kefir grains. After 48 h of fermentation, the pH and total soluble solid content level (TSS) of A and B decreased, while the titratable acidity (TA) value increased. At the end of fermentation, Ave B formulations were stored at 4 °C for 28 days. Fermented sherbets’ 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity decreased with storage. A had 75.3% DPPH radical scavenging activity on day 0th, whereas B had 77.0%. B had less total phenolic content (TPC) than fermented A on day 0. TPC values decreased significantly, notably on days 21st and 28th of storage. After fermentation, A had more gallic acid, hesperidin, p-coumaric acid, rosmarinic acid, and syringic acid than B. A had 2.21% sucrose before fermentation and 0.95% after. On 14th days of storage, potassium and calcium had the greatest mineral levels. Compared to A, B gave higher microbial count values (Lactobacillus sp., Lactococcus sp. total mesophilic aerobic bacteria, and yeast) in the microbiological analysis results at the end of fermentation and during storage.

在这项研究中,无糖(A)和加糖(B)配方的角豆树果冻与水克菲尔颗粒一起发酵。发酵 48 小时后,A 和 B 配方的 pH 值和总可溶性固体含量水平(TSS)下降,而可滴定酸度(TA)值上升。发酵结束后,将 Ave B 配方在 4 °C 下储存 28 天。发酵果冻的 2,2′-二苯基-1-苦基肼(DPPH)自由基清除活性随着储存时间的延长而降低。第 0 天,A 的 DPPH 自由基清除活性为 75.3%,而 B 为 77.0%。在第 0 天,B 的总酚含量(TPC)低于发酵 A。总酚含量明显下降,尤其是在储存的第 21 天和第 28 天。发酵后,A 的没食子酸、橙皮甙、对香豆素、迷迭香酸和丁香酸含量高于 B。贮藏第 14 天时,钾和钙的矿物质含量最高。与 A 相比,B 在发酵末期和贮藏期间的微生物分析结果中微生物数量值(乳酸杆菌、乳球菌、中嗜氧菌和酵母菌总数)更高。
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引用次数: 0
Antioxidant capacity of small black soymilk fermented with ROS-resistant probiotics 用抗 ROS 益生菌发酵的小黑豆浆的抗氧化能力
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-21 DOI: 10.1007/s10068-024-01685-y
Eun-Seo Lim

The purpose of this study was to assess the resistance to reactive oxygen species (ROS) and probiotic properties of lactic acid bacteria (LAB) isolated from naturally fermented sliced radish kimchi, and to evaluate the fermentation characteristics and antioxidant activity of small black soymilk using ROS-resistant probiotic. Leuconostoc dextranicum SRK03, Lactobacillus brevis SRK15, Lactobacillus acidophilus SRK30, and Lactobacillus pentosus SRK38 displayed strong resistance to ROS and demonstrated probiotic activity. The microbiological and physicochemical characteristics and antioxidant activity of small black soymilk fermented with probiotic LAB were significantly higher than those of non-fermented soymilk, and there was a difference in antioxidant activity depending on the probiotic strain. Strong correlations were observed between the antioxidant activity of fermented probiotic soymilk and various parameters. Specific prebiotics promoted the growth of LAB in probiotic-fermented small black soymilk and significantly increased antioxidant activity, suggesting potential functional benefits as a substitute for traditional dairy products.

本研究的目的是评估从天然发酵的萝卜泡菜片中分离的乳酸菌(LAB)对活性氧(ROS)的抗性和益生菌特性,并利用抗ROS益生菌评估小黑豆浆的发酵特性和抗氧化活性。结果表明,白色念珠菌(Leuconostoc dextranicum)SRK03、乳酸杆菌(Lactobacillus brevis)SRK15、嗜酸乳杆菌(Lactobacillus acidophilus)SRK30和戊糖乳杆菌(Lactobacillus pentosus)SRK38对ROS具有很强的抗性,并表现出益生菌活性。用益生菌 LAB 发酵的小黑豆浆的微生物理化特性和抗氧化活性显著高于未发酵豆浆,而且不同益生菌株的抗氧化活性存在差异。发酵益生菌豆浆的抗氧化活性与各种参数之间存在很强的相关性。特定的益生元促进了益生菌发酵小黑豆浆中LAB的生长,并显著提高了抗氧化活性,这表明小黑豆浆作为传统乳制品的替代品具有潜在的功能性益处。
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引用次数: 0
Method development and validation of phloroglucinol and dieckol in Ecklonia cava using HPLC–DAD 利用高效液相色谱-反相分析法(HPLC-DAD)开发和验证 Ecklonia cava 中氯代葡萄糖苷和二羟基甲苯的方法
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-20 DOI: 10.1007/s10068-024-01680-3
Do-Yeon Kim, Hyeon-Ju Park, Choong-In Yun, Young-Jun Kim

The purpose of this study was to simultaneously analyze and validate phlorotannins, a group of functional compounds in Ecklonia cava, using HPLC–DAD. E. cava was profiled using UHPLC-Q-Orbitrap HRMS, and a total of six phlorotannin components were identified. Among these six compounds, phloroglucinol and dieckol were selected as marker components of E. cava, and a simultaneous analysis method using HPLC–DAD for phloroglucinol and dieckol was developed. Specificity, linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), and matrix effect (ME) were verified using HPLC–DAD. These results complied with validation guidelines. The applicability of this simultaneous analysis method was confirmed by quantifying the contents of phloroglucinol and dieckol in E. cava samples (n = 10) purchased in Korea. This method can be used for quality control, such as standardization or determination of compounds in health functional foods containing E. cava extract.

Graphical abstract

本研究的目的是利用 HPLC-DAD 同时分析和验证 Ecklonia cava 中的一类功能性化合物--绿单宁。使用 UHPLC-Q-Orbitrap HRMS 对 Ecklonia cava 进行了分析,共鉴定出六种绿单宁成分。在这六种化合物中,选择了氯代葡萄糖苷醇和二萜醇作为岩藻的标记成分,并建立了一种利用 HPLC-DAD 同时分析氯代葡萄糖苷醇和二萜醇的方法。利用 HPLC-DAD 验证了该方法的特异性、线性、准确度、精密度、检出限(LOD)、定量限(LOQ)和基质效应(ME)。这些结果符合验证指南的要求。通过定量检测在韩国购买的岩藻样品(n = 10)中氯葡萄糖醇和二羟基甲苯的含量,证实了该同时分析方法的适用性。该方法可用于质量控制,如含有岩藻提取物的保健功能食品中化合物的标准化或测定。
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引用次数: 0
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Food Science and Biotechnology
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