Food Science and Biotechnology最新文献

The study investigated the effectiveness of pre- and co-treatment with fermented yellow soybean extract (FYSE) against anti-influenza A virus (IAV) on MDCK cells. FYSE, fermented with Bacillus subtilis, was evaluated for its anti-IAV activity by inhibiting the IAV PA gene expression. Daidzein was identified as a significant contributor to FYSE's antiviral effects. Co-treatment with FYSE and daidzein during IAV infection demonstrated superior anti-IAV activity compared to their respective pre-treatment (IC₅₀: FYSE; 8.65 vs 3.77 µg/mL, and daidzein; 6.01 vs 5.20 µg/mL). Both pre- and co-treatment with FYSE demonstrated higher therapeutic potential than daidzein (Selective index: pre-treatment; > 115.58 vs. 72.32 and co-treatment; > 265.04 vs. 83.56). Despite daidzein showing lower anti-IAV activity in both treatment methods compared to oseltamivir phosphate, it exhibited lower cytotoxicity (CC₅₀: 434.50 vs. 395.20 µg/mL). In conclusion, co-treatment with FYSE and daidzein presents a promising anti-IAV strategy with minimal cytotoxicity in vitro, potentially offering a safer alternative for IAV treatment.

Bisphenol A (BPA) is a representative obesogen that induces adipocyte differentiation and lipid accumulation by mimicking the action of hormones. Brassica juncea is a plant belonging to the mustard family, and has antioxidant, anti-inflammatory, and anti-obesity effects. However, its efficacy against obesogen-induced obesity requires further investigation. In this study, we investigated the anti-obesogenic effects of Brassica juncea extract (BJE) on BPA-treated 3T3-L1 preadipocytes and C57BL/6J mice. The treatment with BPA and BJE did not have cytotoxic effects in vitro. In addition, BJE inhibited BPA-induced lipid accumulation and suppressed BPA-induced changes in adipocyte differentiation, adipogenesis, and lipolysis protein expression in 3T3-L1 cells. Oral administration of BJE reduced body weight, adipose tissue mass, and adipocyte size in BPA-induced obese C57BL/6 mice. BJE also regulated the expression of proteins involved in adipocyte differentiation, adipogenesis, and lipolysis. These data demonstrate that BJE is a natural functional substance with anti-obesogenic effects.

The objective of this study was to produce new active and intelligent high performance colorimetric films based on cellulose acetate (CA), and to evaluate the synergistic effect of thymol (THY) and anthocyanin (ANT). The colour films showed significant reactivity to pH change, and the films became thicker in the presence of glycerol (0.200 ± 0.05). FTIR and SEM images showed a homogeneous distribution and new interactions created between THY, ANT and CA, which modified the thermal properties. This behaviour was also confirmed by XRD analysis, which showed a reduction in film crystallinity with increasing anthocyanin concentration. In addition, the incorporation of ANT improved the mechanical properties by reducing the tensile strength (0.075 ± 0.021 MPa), the biodegradability of the films in the soil after 60 days and also the water vapor transmission rate (14.81 g mm⁻² h⁻¹). The films showed synergistic antibacterial activity and the application trials showed colour changes that were highly visible to the naked eye, with the deterioration of the fish, suggesting a promising application for these films as an indicator of fish freshness.

Strong-aroma Baijiu is a traditional Chinese spirit distilled from grains that undergo solid-state fermentation. At the end of each fermentation cycle, a significant quantity of Huangshui accumulates at the bottom of the fermentation pit, constituting one of the primary by-products in the brewing process of strong-flavor liquor. In this report, the chemical component analysis of Huangshui obtained from a strong-flavor liquor factory revealed a diverse array of organic acids, alcohols, esters, starch, reducing sugars, amino acids, and other compounds present in Huangshui. Additionally, two specialized solid-state fermentation models were developed to investigate the formation process of Huangshui. Our findings demonstrate a positive correlation between the yield of Huangshui and the initial moisture content of fermented grains. It is concluded that Huangshui is the percolate produced during the solid-state brewing process of strong-flavor liquor, with its water content originating from the initial moisture of fermented grains.

Chinese pond turtle muscle peptide's molecular features, purification, structural characteristics, and antioxidant activity were investigated. The Flavourzyme hydrolysate demonstrated greater relative crystallinity (37.53%) than other hydrolysates using X-ray diffraction. The fourier transform infrared spectroscopy spectral changes, the second derivative spectroscopy in the amide-I region (1620–1650 cm⁻¹). Zeta-potential measurement was used to determine the surface charge ranging from − 32.73 to − 28.23 mV. Trypsin hydrolysate obtained the highest solubility (98.72% at pH 1.0) and emulsifying activity (182.81 m² g⁻¹ at pH 7.0), respectively. The Flavourzyme hydrolysate was separated by Sephadex G-10 filtration column chromatography, and three fractions (FH-1, FH-2, and FH-3) were obtained. The molecular weight was < 150 Da in fractions FH-3, FH-2, and FH-1, which were 93.25%, 85.22%, and 76.76%, respectively. The antioxidant activity showed the highest DPPH activity (71.32%) at 7 mg/mL in Fraction FH-2. SEM had a different shape (ball-drop) at FH-2 than the fractions protein (FH-1 and FH-3).

The oral consumption of collagen hydrolysates derived from various animal tissues has been demonstrated to have beneficial effects on skin health, particularly in combating the signs of aging. Here in this study, a novel animal-derived FS-Collagen hydrolysates were developed and its effects against skin aging was analyzed in a new mice skin aging model established through a combination of UV irradiation and d-galactose induction. 8 Weeks of oral FS-Collagen administration demonstrated significant protective effects against skin aging in mice, evidenced by the preservation of the skin’s macroscopic appearance, the restoration of skin composition and structure, an enhancement in antioxidant capacity and the inhibition of inflammation. Additionally, FS-Collagen safeguards skin barrier integrity and cellular connections, particularly by maintaining the expression levels of Dsg1 and Jam-A. In summary, the FS-Collagen exhibits positive effects in countering skin aging and holds promise as an alternative functional nutrition supplement for anti-skin aging care.

Liquid chromatography-diode array detection (HPLC-DAD) and ultra-high-performance liquid chromatography (UHPLC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) methods were investigated for the simultaneous determination of five bioactive compounds in the rhizome and tuberous root of Curcuma longa L. (turmeric). These belonging to the two chemical groups: three curcuminoids (curcumin, bisdemethoxycurcumin, and demethoxycurcumin) and two sesquiterpenoids (ar-turmerone and bisacurone). The established analytical method was validated based on AOAC guidelines for specificity, linearity, accuracy, and precision. Additionally, the system suitability test and measurement uncertainty were also estimated. The validated method was successfully applied to evaluate the quality of 25 commercial turmeric samples. This proposed method can be applied in practice for the quality control indicator of turmeric, such as its functionality and stability. Therefore, the development of this analytical method represents a significant advancement in the quality assessment of turmeric, offering a valuable tool for food industries reliant on this particular spice and ingredient.