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Beneficial effects of Tteokbokki sauce meeting the functional labeling system claim on dexamethasone-induced C2C12 myotube and hindlimb immobilization-induced C57BL/6J mice 符合功能性标记系统要求的手抓饼酱对地塞米松诱导的 C2C12 肌管和后肢固定诱导的 C57BL/6J 小鼠的有益作用
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-03 DOI: 10.1007/s10068-024-01675-0
Geon Oh, Xionggao Han, Xiao Men, Ji-Hyun Im, Xiaolu Fu, June-Seok Lim, Luri Lee, Min Hee Kwon, Ho-Seong Lee, Moon Jin Ra, Sun-Il Choi, Ok-Hwan Lee

This study investigated the potential of tteokbokki sauce meeting the functional labeling system (TSF) with resistant maltodextrin on muscle atrophy using dexamethasone-induced C2C12 myotubes and hindlimb immobilization (HI)-induced C57BL/6J mice. TSF significantly improved cell viability, myotube diameter, and fusion index in C2C12 myotubes. Furthermore, TSF effectively mitigated HI-induced muscle atrophy in mice, enhanced grip strength, and increased muscle mass. At the molecular level, TSF upregulated proteins related to muscle protein synthesis (IGF-1/Akt/mTOR) and mitochondrial biogenesis (SIRT1/PGC-1α), while downregulating proteins associated with muscle protein degradation (Fbx32/FoxO3a/MuRF1). This dual action highlights the potential of TSF to promote muscle health and prevent muscle atrophy. Our findings suggest that TSF holds promise as a functional food ingredient for treating muscle atrophy. Developing a sauce with anti-muscle atrophy properties opens new avenues for creating novel health-promoting food products.

本研究使用地塞米松诱导的 C2C12 肌细胞管和后肢固定(HI)诱导的 C57BL/6J 小鼠,研究了符合功能性标记系统(TSF)的 Tteokbokki 酱与抗性麦芽糊精对肌肉萎缩的潜在影响。TSF能明显提高C2C12肌管的细胞活力、肌管直径和融合指数。此外,TSF 还能有效缓解 HI 诱导的小鼠肌肉萎缩,增强握力并增加肌肉质量。在分子水平上,TSF 上调了与肌肉蛋白质合成(IGF-1/Akt/mTOR)和线粒体生物生成(SIRT1/PGC-1α)相关的蛋白质,同时下调了与肌肉蛋白质降解(Fbx32/FoxO3a/MuRF1)相关的蛋白质。这种双重作用凸显了 TSF 促进肌肉健康和防止肌肉萎缩的潜力。我们的研究结果表明,TSF有望成为治疗肌肉萎缩的功能性食品配料。开发具有抗肌肉萎缩特性的酱料为创造新型健康食品开辟了新途径。
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引用次数: 0
Interaction of dairy and plant proteins for improving the emulsifying and gelation properties in food matrices: a review 乳制品和植物蛋白相互作用改善食品基质的乳化和凝胶特性:综述
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-01 DOI: 10.1007/s10068-024-01671-4
Srutee Rout, Pranjyan Dash, Pradeep Kumar Panda, Po-Chih Yang, Prem Prakash Srivastav

A variety of variables influence food texture, two of which are gelation and emulsification. Protein interactions have an important role in influencing gelation and emulsifying properties. The utilization of plant proteins in the development of food systems is a prominent subject within the current protein transition paradigm. Plant proteins diminish gel strength compared to dairy proteins. Protein providers prefer to create their own networks rather than rely on tight ties. It may be feasible to resolve these challenges if the interactions between plant and dairy proteins are known at all sizes, from molecular to macroscopic. Therefore, the proteins and dairy proteins are the main emphasis of this review. The role of these proteins in interacting with food matrices is also discussed. Additionally, this data gives information on worldwide research trends. Finally, a glimpse into the future was discussed.

影响食物质地的变量有很多,凝胶化和乳化是其中的两个。蛋白质之间的相互作用在影响凝胶和乳化特性方面发挥着重要作用。利用植物蛋白开发食品系统是当前蛋白质转型范例中的一个重要课题。与乳制品蛋白相比,植物蛋白会降低凝胶强度。蛋白质供应商更愿意创建自己的网络,而不是依赖紧密的联系。如果能够了解植物蛋白和乳制品蛋白之间从分子到宏观各个层面的相互作用,也许就能解决这些难题。因此,蛋白质和乳制品蛋白质是本综述的重点。本综述还讨论了这些蛋白质与食品基质相互作用的作用。此外,这些数据还提供了有关全球研究趋势的信息。最后,还讨论了对未来的展望。
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引用次数: 0
Antioxidant activity and anti-inflammatory effect of solvent fractions from Chinese cabbage roots (Brassica rapa L. ssp. pekinensis) 大白菜根(Brassica rapa L. ssp. pekinensis)溶剂馏分的抗氧化活性和抗炎作用
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-01 DOI: 10.1007/s10068-024-01652-7
Byung-Min Oh, Hyeon Hwa Oh, Kyung Eun Moon, So-Won Jang, Geun-Seoup Song

In this study, Chinese cabbage (Brassica rapa L. ssp. pekinensis) roots were solvent fractioned, and their antioxidant and anti-inflammatory effects were investigated. The antioxidant capacity (DPPH and ABTS radicals, oxygen radical absorbance capacity, and cupric reducing antioxidant capacity) was the highest in the ethyl acetate fraction (CREE) at 26.74, 69.81, 253.23, and 54.77 mg TEAC/g, respectively. The inflammatory responses were evaluated in RAW 264.7 cells stimulated with lipopolysaccharide (1 µg/mL). CREE decreased nitric oxide and prostaglandin E2 by 53.37% and 16.30%, respectively. Pro-inflammatory cytokines such as tumor necrosis factor-α, interleukin-1β, and interleukin-6 were inhibited by 36.85%, 62.99%, and 54.78%, respectively. Furthermore, inducible nitric oxide synthase, cyclooxygenase-2, and interleukin-6 genes were inhibited by 43.38%, 24.23%, and 80.85%, respectively. The results suggest that CREE is responsible for its antioxidant and anti-inflammatory effects.

本研究对大白菜(Brassica rapa L. ssp. pekinensis)根进行了溶剂分馏,并研究了其抗氧化和抗炎作用。乙酸乙酯馏分(CREE)的抗氧化能力(DPPH 和 ABTS 自由基、氧自由基吸收能力和铜还原抗氧化能力)最高,分别为 26.74、69.81、253.23 和 54.77 mg TEAC/g。用脂多糖(1 微克/毫升)刺激 RAW 264.7 细胞,对炎症反应进行了评估。CREE 可使一氧化氮和前列腺素 E2 分别减少 53.37% 和 16.30%。肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6等促炎细胞因子的抑制率分别为 36.85%、62.99% 和 54.78%。此外,诱导型一氧化氮合酶、环氧化酶-2 和白细胞介素-6 基因分别被抑制了 43.38%、24.23% 和 80.85%。结果表明,CREE 具有抗氧化和抗炎作用。
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引用次数: 0
Physical and rheological properties of agglomerated milk protein isolate–guar gum mixtures: effect of binder type and concentration 凝集的牛奶分离蛋白-瓜尔胶混合物的物理和流变特性:粘合剂类型和浓度的影响
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-31 DOI: 10.1007/s10068-024-01659-0
Jun-Hwan Oh, Subin An, Juneha Bak, Byoungseung Yoo

Our study sought to investigate how the fluidized-bed agglomeration process, incorporating sugar and sugar alcohol binders, affects the physical and rheological properties of milk protein isolate (MPI)–guar gum (GG) mixtures. The agglomerated MPI-GG mixtures (AMGs) consisted of larger and more porous particles with a uniform particle size distribution and showed better solubility compared to non-agglomerated MG (NMG). Additionally, the agglomeration process with binders improved powder flowability by reducing powder cohesion. However, AMGs exhibited lower apparent viscosity and viscoelastic moduli than NMG, and these values decreased with higher binder concentrations because the presence of sugar and sugar alcohol binders promoted the depletion interaction between MPI and GG. This finding was supported by confocal laser scanning microscopic images, which showed more and larger GG lumps in AMGs than in NMG. Our findings may contribute to the utilization of AMGs in various foods including dairy products.

我们的研究旨在探讨加入糖和糖醇粘合剂的流化床团聚工艺如何影响牛奶蛋白分离物(MPI)-瓜尔胶(GG)混合物的物理和流变特性。聚结后的 MPI-GG 混合物(AMG)颗粒更大、更多孔、粒度分布更均匀,与未聚结的 MG(NMG)相比,溶解性更好。此外,带有粘合剂的团聚过程可通过降低粉末内聚力来改善粉末流动性。然而,AMG 的表观粘度和粘弹性模量低于 NMG,并且这些值随着粘合剂浓度的增加而降低,这是因为糖和糖醇粘合剂的存在促进了 MPI 和 GG 之间的耗竭作用。共焦激光扫描显微图像也支持这一发现,图像显示 AMG 中的 GG 块体比 NMG 中的更多更大。我们的发现可能有助于在包括乳制品在内的各种食品中利用 AMG。
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引用次数: 0
Development of egg yolk-free mayonnaise using rice protein with xanthan gum 利用大米蛋白和黄原胶研制不含蛋黄的蛋黄酱
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-30 DOI: 10.1007/s10068-024-01672-3
Manhee Baek, Sujin Jeon, Semin Jeong, Saehun Mun

In this study, egg yolk-free mayonnaise was produced by replacing egg yolk with rice protein (RP) and the preparation conditions were standardized to obtain rheological properties similar to those of commercial mayonnaise. Specifically, RP was isolated at pH 2 to address its insolubility issues in acidic conditions, aiming to enable direct application in acidic foods such as mayonnaise. All mayonnaise samples showed shear thinning properties, and the yield stress increased with the increase in RP and xanthan gum (XG) content, compared to mayonnaise containing egg yolk. Moreover, comparing the viscoelasticity of commercial mayonnaise with that of mayonnaise containing 0.1 or 0.2 wt% XG revealed an increase in storage modulus and loss modulus with the rise in RP and XG content. These results confirmed that RP isolated at pH 2 could be used to prepare mayonnaise as a natural emulsifier to replace egg yolk.

在这项研究中,通过用大米蛋白质(RP)替代蛋黄,生产出了不含蛋黄的蛋黄酱,并对制备条件进行了标准化,以获得与商用蛋黄酱相似的流变特性。具体来说,大米蛋白质在 pH 值为 2 时被分离出来,以解决其在酸性条件下不溶解的问题,目的是将其直接应用于蛋黄酱等酸性食品中。与含有蛋黄的蛋黄酱相比,所有蛋黄酱样品都具有剪切变稀的特性,并且屈服应力随着 RP 和黄原胶(XG)含量的增加而增加。此外,将商用蛋黄酱的粘弹性与含有 0.1 或 0.2 wt% XG 的蛋黄酱的粘弹性进行比较后发现,随着 RP 和 XG 含量的增加,储存模量和损失模量也随之增加。这些结果证实,在 pH 值为 2 的条件下分离出的 RP 可用于制作蛋黄酱,作为替代蛋黄的天然乳化剂。
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引用次数: 0
Improvement of properties and olfactory attributes of isolated protein from edible insects by roasting 通过焙烧改善食用昆虫分离蛋白的特性和嗅觉属性
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-29 DOI: 10.1007/s10068-024-01667-0
Chinbat Ganbat, Jung-Ah Han

The characteristics of proteins extracted from two kinds of edible insects (Gryllus Bimaculatus and Tenebrio Molitor, for G.B and T.M, respectively) were compared after roasting at 180 °C for 15 min and 200 °C for 10 min, respectively. The amino acid content decreased by roasting, and the degree of decrease varied depending on the type of edible insect and roasting temperature. Antioxidant activity increased by 5.2–11.3% following roasting, with no significant differences by roasting temperature. The results of Infrared (IR) spectrum and gas chromatography (GC) analysis revealed that compounds contributing to a strong waxy scent and sour taste decreased, whereas those associated with aroma and floral scent increased as a result of roasting. In conclusion, roasting led to an enhancement in the olfactory characteristics of proteins extracted from edible insects, and roasting at 180 °C for 20 min for G.B and 200 °C for 15 min for T.M could be considered optimal.

比较了从两种食用昆虫(Gryllus Bimaculatus 和 Tenebrio Molitor,分别为 G.B 和 T.M)中提取的蛋白质在 180 ℃ 烘烤 15 分钟和 200 ℃ 烘烤 10 分钟后的特性。焙烧后氨基酸含量降低,降低程度因食用昆虫种类和焙烧温度而异。焙烧后,抗氧化活性提高了 5.2-11.3%,不同焙烧温度下的差异不显著。红外光谱(IR)和气相色谱(GC)分析结果表明,焙烧后,产生强烈蜡香味和酸味的化合物减少了,而与香气和花香有关的化合物增加了。总之,焙烧可提高从食用昆虫中提取的蛋白质的嗅觉特性,G.B 的焙烧温度为 180 ℃ 20 分钟,T.M 的焙烧温度为 200 ℃ 15 分钟,这两种焙烧温度都是最佳的。
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引用次数: 0
Food carbohydrates in the gut: structural diversity, microbial utilization, and analytical strategies 肠道中的食物碳水化合物:结构多样性、微生物利用和分析策略
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-26 DOI: 10.1007/s10068-024-01648-3
HyunJi Lee, JaeHui Song, Bokyung Lee, Jaeho Cha, Hyeyoung Lee

Carbohydrates, which are a vital dietary component, undergo digestion and gut fermentation through microbial enzymes to produce beneficial short-chain fatty acids. Certain carbohydrates selectively modulate the gut microbiota, impacting host health. Carbohydrate-active enzymes within the gut microbiota significantly contribute to carbohydrate utilization and microbial diversity. Despite their importance, the structural complexity of carbohydrates poses analytical challenges. However, recent advancements, notably, mass spectrometry, have allowed for their characterization and functional analysis. This review examines the intricate relationship between dietary carbohydrates and the gut microbiota, highlighting the crucial role of advanced analytical techniques in understanding their diversity and implications. These advancements provide valuable insights into carbohydrate bioactivity. Integrating high-throughput analysis with next-generation sequencing provides deeper insights into gut microbial interactions, potentially revealing which carbohydrate structures are beneficial for gut health.

碳水化合物是重要的膳食成分,通过微生物酶进行消化和肠道发酵,产生有益的短链脂肪酸。某些碳水化合物会选择性地调节肠道微生物群,从而影响宿主的健康。肠道微生物群中的碳水化合物活性酶大大促进了碳水化合物的利用和微生物的多样性。尽管碳水化合物非常重要,但其结构的复杂性给分析带来了挑战。然而,最近的进步,尤其是质谱技术的进步,使得对碳水化合物的表征和功能分析成为可能。这篇综述探讨了膳食碳水化合物与肠道微生物群之间错综复杂的关系,强调了先进分析技术在了解其多样性和影响方面的关键作用。这些进步为了解碳水化合物的生物活性提供了宝贵的见解。将高通量分析与新一代测序技术相结合,可以更深入地了解肠道微生物之间的相互作用,从而揭示哪些碳水化合物结构有益于肠道健康。
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引用次数: 0
Fatty acids and epigenetics in health and diseases 脂肪酸与健康和疾病中的表观遗传学
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-25 DOI: 10.1007/s10068-024-01664-3
Min-Yu Chung, Byung Hee Kim

Lipids are crucial for human health and reproduction and include diverse fatty acids (FAs), notably polyunsaturated FAs (PUFAs) and short-chain FAs (SCFAs) that are known for their health benefits. Bioactivities of PUFAs, including ω-6 and ω-3 FAs as well as SCFAs, have been widely studied in various tissues and diseases. Epigenetic regulation has been suggested as a significant mechanism affecting the progression of various diseases, including cancers and metabolic and inflammatory diseases. Epigenetics encompasses the reversible modulation of gene expression without altering the DNA sequence itself, mediated by mechanisms such as DNA methylation, histone acetylation, and chromatin remodeling. Bioactive FAs have been demonstrated to regulate gene expression via epigenetic modifications that are potentially important for modulating metabolic control and disease risk. This review paper discusses the evidence in support of bioactive FAs, including ω-6 and ω-3 FAs and SCFAs, eliciting various disease prevention via epigenetic regulation including methylation or acetylation.

Graphical abstract

脂质对人体健康和生殖至关重要,其中包括多种脂肪酸(FAs),特别是多不饱和脂肪酸(PUFAs)和短链脂肪酸(SCFAs),它们对健康有益。人们广泛研究了多元不饱和脂肪酸(包括 ω-6 和 ω-3 脂肪酸以及 SCFAs)在各种组织和疾病中的生物活性。表观遗传调控被认为是影响各种疾病(包括癌症、代谢性疾病和炎症性疾病)进展的重要机制。表观遗传学包括在不改变 DNA 序列本身的情况下,通过 DNA 甲基化、组蛋白乙酰化和染色质重塑等机制对基因表达进行可逆调节。生物活性脂肪酸已被证明可通过表观遗传修饰调节基因表达,这对调节代谢控制和疾病风险具有潜在的重要意义。本综述论文讨论了支持生物活性脂肪酸(包括ω-6和ω-3脂肪酸以及SCFAs)通过包括甲基化或乙酰化在内的表观遗传调控引发各种疾病预防的证据。
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引用次数: 0
Isopimpinellin inhibits UVA-induced overproduction of MMPs via suppression of MAPK/AP-1 signaling in human dermal fibroblasts 异柚皮素通过抑制人真皮成纤维细胞中的 MAPK/AP-1 信号传导,抑制 UVA 诱导的 MMPs 过度生成
IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-24 DOI: 10.1007/s10068-024-01611-2
Jung Hwan Oh, Fatih Karadeniz, Youngwan Seo, Chang-Suk Kong

Corydalis heterocarpa is an edible halophyte and an ingredient in traditional Korean medicine. In the present study, isopimpinellin (IPN), a bioactive coumarin, was isolated from the medicinal halophyte C. heterocarpa, and the effects of IPN against UVA-induced photoaging were investigated in human dermal fibroblasts. Photoaging is a skin disorder that manifests itself as premature skin aging due to chronic exposure to UV radiation. The symptoms of photoaging mainly arise from degraded skin connective tissue produced by overly expressed matrix metalloproteinases (MMPs). IPN treatment decreased the UVA-induced formation of reactive oxygen species and decreased MMP-1, MMP-3, and MMP-9 collagenases at the protein level. The UVA-mediated suppression of tissue inhibitors of MMP-1 and -2 was attenuated with IPN. The presence of 10 μM IPN inhibited the MAPK-mediated phosphorylation of c-Fos and c-Jun. In conclusion, the overall result of the current study indicated that IPN inhibited the UVA-induced overexpression of MMPs via blocking the MAPK/AP-1 pathway.

苍术是一种可食用的卤叶植物,也是韩国传统药物的一种成分。本研究从药用裙带菜中分离出了具有生物活性的香豆素--异茴香豆素(IPN),并研究了 IPN 在人类真皮成纤维细胞中对 UVA 诱导的光老化的影响。光老化是一种皮肤疾病,表现为由于长期暴露于紫外线辐射而导致的皮肤过早老化。光老化的症状主要源于基质金属蛋白酶(MMPs)过度表达导致的皮肤结缔组织退化。IPN 治疗可减少 UVA 诱导的活性氧的形成,并在蛋白质水平上减少 MMP-1、MMP-3 和 MMP-9 胶原酶。IPN 可减轻 UVA 介导的对 MMP-1 和 MMP-2 组织抑制剂的抑制。10 μM IPN 的存在抑制了 MAPK 介导的 c-Fos 和 c-Jun 磷酸化。总之,本研究的总体结果表明,IPN 通过阻断 MAPK/AP-1 通路抑制了 UVA 诱导的 MMPs 过表达。
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引用次数: 0
A comparative study of extraction methods for recovery of bioactive components from brown algae Sargassum serratifolium 从褐藻马尾藻中提取生物活性成分的方法比较研究
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-23 DOI: 10.1007/s10068-024-01649-2
Su Hyeon Baek, Joo Won Lee, Truc Cong Ho, Yena Park, Shymaa M. Ata, Hyun Jung Yun, Gyoungok Gang, Adane Tilahun Getachew, Byung-Soo Chun, Sang Gil Lee, Lei Cao

Species of Sargassum genus are known to be rich sources of bioactive compounds. However, there is a lack of studies comparing extraction methods for these bioactive components. This study aimed to compare the total phenolic contents, total antioxidant capacity, tyrosinase inhibitory effect, sargahydroquinoic acid (SHQA) and sargachromenol (SCM), two algal meroterpenoids, of Sargassum serratifolium extracts acquired by different extraction methods. The methods employed in this study included conventional solid–liquid extraction using methanol (SME), supercritical fluid extraction using CO2 with ethanol as a co-solvent (SC-CO2 + ethanol), and pressurized liquid extraction (PLE) at two temperatures (25 and 100 °C). PLE at 100 °C (PLE100) exhibited the highest total yield, total phenolic content, total antioxidant capacity and tyrosinase inhibitory activity. Notably, SME resulted in the highest recovery of both SHQA and SCM. Compared to SME, PLE100 exhibited a two-fold increase in antioxidant capacity but a minimal increase in phenolic content.

众所周知,马尾藻是生物活性化合物的丰富来源。然而,目前还缺乏对这些生物活性成分的提取方法进行比较的研究。本研究旨在比较马尾藻萃取物中的总酚含量、总抗氧化能力、酪氨酸酶抑制作用、马尾藻氢喹啉酸(SHQA)和马尾藻酚(SCM)这两种藻类美拉特萜类化合物在不同萃取方法下的含量。本研究采用的方法包括使用甲醇的传统固液萃取法(SME)、使用二氧化碳和乙醇作为辅助溶剂的超临界流体萃取法(SC-CO2 + 乙醇)以及两种温度(25 °C和100 °C)的加压液体萃取法(PLE)。100 °C下的加压液体萃取(PLE100)显示出最高的总产量、总酚含量、总抗氧化能力和酪氨酸酶抑制活性。值得注意的是,SME 使 SHQA 和 SCM 的回收率最高。与 SME 相比,PLE100 的抗氧化能力提高了两倍,但酚类含量的提高幅度很小。
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引用次数: 0
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