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Comparison of Insecticide Resistance and Its Enzyme Mechanisms among Aedes aegypti Collected with Three Methods in a Dengue-Endemic City in Southern Mexico 墨西哥南部某登革热流行城市三种方法采集的埃及伊蚊抗药性及其酶机制比较
Pub Date : 2022-01-01 DOI: 10.4236/ae.2022.103018
Walter Eduardo Quezada-Yaguachi, Américo D. Rodríguez, Francisco Solis-Santoyo, Alma D López-Solis, W. Black Iv, Karla Saavedra-Rodriguez, Diego Morales-Viteri, R. P. Penilla-Navarro
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引用次数: 0
Field Exploration of the Efficacy of Some Friendly Products in Combination with Some Pesticides against the Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) and Its Effect on Total Carbohydrates and Micronutrients in the Resulting Date Fruits 几种友好产品联合农药对红棕象甲(鞘翅目:Curculionidae)的田间防治效果及对红枣果实中总碳水化合物和微量元素的影响
Pub Date : 2022-01-01 DOI: 10.4236/ae.2022.104020
M. I. Mogahed
Experiments were carried out against the red palm weevil, Rhynchophorus ferrugineus (Oliv.) (RPW in a private palm orchard containing varieties of Samani (domestic) and Ikhlas (imported) infested with the red palm weevil Rhynchophorus ferrugineus (Oliv.) (RPW) in El-Marg area, Cairo Governorate, Egypt, using Petroleum oil, Jojoba oil, and Inorganic salts, singly and mixed with each of insecticides (Acetamiprid, Profenofos, Dimethoate) by injection and spraying of infested palm trunks, as well as pouring these substances into pits around the roots of the affected palm trees. The data showed that Acetamiprid was the most effective against RPW. The effective mixtures against R. ferrugineus were Jujuba oil with Acetamiprid, also used car oil with inorganic salts, which resulted in 100% recovery of treated palm. Continuing treatment of infested palms for 6 consecutive months by injecting used car oil mixed with inorganic saline solution resulted in 100% recovery for both date palm cultivars. Spraying these substances on the affected trunks had no effect on RPW. Pouring the tested solutions around the roots of the affected palm had the least effect against RPW. Injection of the tested pesticides into the ganic salt, compared to the control group. There is a significant difference between the treatments.
在埃及开罗省El-Marg地区的一个私人棕榈园内,用石油、荷荷巴油和无机盐单独或与杀虫剂(醋氨虫、丙烯虫、乐果)混合,对红棕榈象(Rhynchophorus ferrugineus (Oliv.)) (RPW .)进行了防治试验,该试验含有被红棕榈象(Rhynchophorus ferrugineus, Oliv.) (RPW .)侵染的Samani(国产)和Ikhlas(进口)品种。并将这些物质倒入受影响棕榈树根部周围的坑中。结果表明,扑热息痛对RPW的防治效果最好。枣树油与醋氨虫啉、汽车油与无机盐的混合处理对铁瘟螨有较好的防治效果,处理后的棕榈回收率为100%。连续6个月注射混合无机盐溶液的二手车油处理侵染棕榈树,两种椰枣品种的回收率均为100%。在受影响的树干上喷洒这些物质对RPW没有影响。在受影响的手掌根部周围倾倒测试溶液对RPW的影响最小。将试验农药注射到有机盐中,与对照组相比。两种治疗方法之间存在显著差异。
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引用次数: 1
Anopheles leesoni Evans 1931, a Member of the Anopheles funestus Group, Is a Potential Malaria Vector in Cameroon 利斯尼埃文斯按蚊1931,一种非氏按蚊群的成员,是喀麦隆潜在的疟疾媒介
Pub Date : 2022-01-01 DOI: 10.4236/ae.2022.101008
E. Kopya, C. Ndo, L. Djamouko-Djonkam, L. Nkahe, P. Awono-ambene, F. Njiokou, Charles Sinclair Wondji, C. Antonio-Nkondjio
Background: Understanding the biology of Anopheles malaria vector species is essential to planning effective and sustainable malaria control strategies in endemic countries. This study reported the implication of Anopheles leesoni in malaria transmission in Cameroon, Central Africa. Methods: Mosquitoes were collected in three localities from May 2015 to March 2018 using electric aspirators and Centers for Disease Control light traps (CDC-LT). Anopheles funestus sensu lato (s.l.) mosquitoes were identified as species using polymerase chain reaction assay (PCR). Furthermore, Plasmodium falciparum infection status was determined using the enzyme-linked immunosorbent assay (ELISA) leesoni was found positive for P. falciparum (infection rate: 10.98%) in Cameroon. Conclusion: A very high P. falciparum infection rate was observed in this study in A. funestus s.s., highlighting its high implication in malaria transmission in Cameroon. Furthermore, the detection of P. falciparum infection in A. leesoni calls for more attention towards this neglected vector species.
背景:了解疟媒按蚊的生物学特性对疟疾流行国家制定有效和可持续的疟疾控制策略至关重要。本研究报道了李氏按蚊在中非喀麦隆疟疾传播中的意义。方法:2015年5月- 2018年3月,采用电诱蚊器和美国疾病控制与预防中心诱蚊灯(CDC-LT)在3个地点采集蚊虫。采用聚合酶链式反应法(PCR)鉴定了褐感按蚊属。此外,采用酶联免疫吸附试验(ELISA)检测恶性疟原虫感染状况,发现喀麦隆leesoni恶性疟原虫阳性(感染率为10.98%)。结论:在本研究中发现了恶性疟原虫在喀麦隆的高感染率,表明其在喀麦隆疟疾传播中具有重要意义。此外,在李氏伊蚊中检测到恶性疟原虫感染,需要更多地关注这一被忽视的病媒物种。
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引用次数: 2
Ecology, Distribution and Risk of Transmission of Viral Haemorrhagic Fevers by Aedes Mosquitoes around the Port Areas of Tema in Southern Ghana 加纳南部特马港区伊蚊传播病毒性出血热的生态、分布和风险
Pub Date : 2022-01-01 DOI: 10.4236/ae.2022.102010
Kenechukwu C. Ojukwu, J. Chabi, Kwadwo K. Frempong, D. Adabie-Gomez, Joseph Harold Nyarko Osei, A. Asafu-Adjaye, Sellase Pi-Bansa, Michelle Adimazoya, Kojo Y. Sakyi, Kwaku O. Akuoko, Ruth C. Brenyah, Maxwell A. Appawu, Samuel Kweku Dadzie
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引用次数: 2
Effect of Different Nethouses on the Incidence of Insect on Vegetable Crops 不同蜂巢对蔬菜作物昆虫发生的影响
Pub Date : 2022-01-01 DOI: 10.4236/ae.2022.103016
A. Quamruzzaman, F. Islam, L. Akter, M. Hossain, Sharmilla Rani Mallick
In this study, the effects of different types of nethouses viz., NH1, NH2, and NH3, were investigated at the research farm of the Olericulture Division, Bangladesh Agricultural Research Institute (BARI) from Sep 2021 to Mar 2022 and compared to control (open field) in terms of the incidence of white fly, aphid, leaf miners, mites, and eggplant shoot and fruit borer (ESFB). This study included six vegetables 1) Tomato, 2) Eggplant, 3) Sweet pepper, 4) Broccoli, 5) Cucumber; 6) Okra, as well as four nethouse treatments: 1) UV stabilized transparent polyethylene film with 60 mesh insect net along with green shade net (NH1); 2) UV stabilized transparent polyethylene film with 60 mesh insect net (NH2); 3) 60 mesh insect net (NH3); 4) Open field infestation of white fly, aphid, leaf miners, mites, and ESFB was observed under open field conditions followed by NH1 conditions, while the lower infestation was observed in NH2 and NH3 for all vegetables. From the study, we found the use of stabilized transparent polyethylene film with a 60 mesh insect nethouse provided a negative effect on the presence of different types of notorious insects on vegetables. So, this type of protected nethouse will provide a new dimension to producing safe and quality vegetables in Bangladesh.
本研究于2021年9月至2022年3月在孟加拉国农业研究所(BARI)油菜栽培部研究农场调查了不同类型的蜂巢(NH1、NH2和NH3)的影响,并与对照(露天)进行了白蛉、蚜虫、采叶虫、螨虫和茄子笋和果螟(ESFB)的发病率比较。这项研究包括6种蔬菜:1)西红柿,2)茄子,3)甜椒,4)西兰花,5)黄瓜;6)秋葵,以及四种网棚处理:1)紫外线稳定透明聚乙烯膜,60目防虫网和绿色遮阳网(NH1);2)紫外线稳定透明聚乙烯薄膜,60目防虫网(NH2);3) 60目防虫网(NH3);4)露天蔬菜中白蝇、蚜虫、采叶虫、螨虫和ESFB的侵染程度在露天条件下次之,在NH1条件下次之,而在NH2和NH3条件下所有蔬菜的侵染程度均较低。从研究中,我们发现使用稳定透明聚乙烯薄膜和60目昆虫网对蔬菜上不同类型的臭名昭着的昆虫的存在产生了负面影响。因此,这种受保护的网房将为孟加拉国生产安全和高质量的蔬菜提供一个新的层面。
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引用次数: 0
Finding Food Security through Changing the Agricultural Model to Sustain Insect Biodiversity 通过改变农业模式维持昆虫生物多样性寻找粮食安全
Pub Date : 2021-06-10 DOI: 10.4236/ae.2021.93011
A. Jankielsohn
Worldwide biodiversity is being threatened by human activities to a greater level wherein the natural ecosystems are reaching the verge of collapsing. We are faced with four major interrelated challenges namely a changing climate, biodiversity loss, human population growth and food production for this growing population. Agricultural intensification contributes significantly to biodiversity loss. The agricultural model for our current food production systems is mainly based on the Green Revolution, which promoted the cultivation of crops in extensive monoculture fields and intensified external inputs of agrochemicals. This model resulted in biodiversity loss, particularly in insect populations. A model based on ecological intensification as an alternative to agricultural intensification with minimized use of agro-inputs may slow the rate of biodiversity loss resulting in more sustainable agricultural ecosystems.
世界范围内的生物多样性正受到人类活动的威胁,自然生态系统正处于崩溃的边缘。我们面临着四个相互关联的重大挑战,即气候变化、生物多样性丧失、人口增长和为不断增长的人口生产粮食。农业集约化是生物多样性丧失的重要原因。我们当前粮食生产系统的农业模式主要基于绿色革命,它促进了在广泛的单一栽培领域种植作物,并加强了农用化学品的外部投入。这种模式导致了生物多样性的丧失,尤其是昆虫种群的丧失。以生态集约化为基础的模式作为农业集约化的替代方案,尽量减少农业投入物的使用,可以减缓生物多样性丧失的速度,从而实现更可持续的农业生态系统。
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引用次数: 0
The Biology of Szelenyiopria talitae (Hymenoptera: Diapriidae): Larval Parasitoid of the Leaf-Cutting Ant Acromyrmex subterraneus (Hymenoptera: Formicidae) 切叶蚁(膜翅目:蚁科)幼虫寄生蜂的生物学研究
Pub Date : 2021-06-10 DOI: 10.4236/ae.2021.93012
T. C. Mattoso, Denise Dolores Oliveira Moreira, T. Teodoro, Claudio Luiz Melo Souza, Rita de Kássia Guarnier da Silva, V. Morais, Carlos P. Silva, M. Erthal, R. Samuels
The biology of a koinobiont parasitoid of leaf-cutting ant larvae, Szelenyiopria talitae (Hymenoptera: Diapriidae), was studied from naturally infested Acromyrmex subterraneus (Hymenoptera: Formicidae) nests. Nests were collected in the field from the Atlantic rainforest biome in the state of Rio de Janeiro. A total of fifty-three nests were collected from 2015 to 2018. Parasitized nests were only found during the months of September and October. Approximately 22% of the nests collected over a four-year period were found to have been parasitized by S. talitae. The mean within-nest parasitism rate was 66.3%. This diapriid displayed both solitary (14%) and gregarious parasitism (86%), with up to a maximum of 12 parasitoids developing within a single host. Gregarious parasitism with two (29%) or three (21%) S. talitae per host was most frequently observed. There was a positive correlation between the number of parasitoids per host and host size (dry weight), indicating that S. talitae females oviposited a higher number of eggs in larger hosts. There was also a negative correlation between S. talitae pharate adult size and the number of parasitoids per host, which could have been caused by sibling competition for limited host resources. The high levels of parasitism seen here had a debilitating effect on the colonies. Acromyrmex subterraneus is a serious pest in Brazil, and these studies lay the foundation for understanding the impact of S. talitae on ant populations.
从自然感染的Acromyrmex subterraneus(膜翅目:蚁科)巢穴中,研究了一种切叶蚁幼虫的寄生蜂Szelenyiopria talitae(膜翅目:Diapridae)的生物学特性。鸟巢是从里约热内卢州的大西洋雨林生物群落中采集的。从2015年到2018年,共收集了五十三个巢穴。寄生巢穴只在9月和10月才被发现。在四年的时间里收集到的巢穴中,大约有22%被意大利猪笼草寄生。平均巢内寄生率为66.3%,该寄生蜂既表现为孤立寄生(14%),又表现为群居寄生(86%),单个寄主内最多可发育12种寄生蜂。最常观察到每个宿主有两个(29%)或三个(21%)意大利S.talitae的Gregarious寄生。每个宿主的寄生蜂数量与宿主大小(干重)呈正相关,这表明意大利S.talitae雌性在较大的宿主中产卵数量较多。塔氏锥虫成虫大小与每个寄主的寄生蜂数量之间也存在负相关,这可能是由于兄弟姐妹争夺有限的寄主资源造成的。这里看到的高水平寄生对群落产生了削弱作用。Acrommyrmex subterraneus是巴西的一种严重害虫,这些研究为了解意大利蚁对蚂蚁种群的影响奠定了基础。
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引用次数: 0
Comprehensive Value Chain Development of Natural Resources for Economic Diversification: The Apiculture Approach 面向经济多元化的自然资源综合价值链开发:养蜂方法
Pub Date : 2021-03-01 DOI: 10.4236/AE.2021.92006
A. A. Oyerinde, T. Omara-Achong
The present economic challenges in Nigeria call for a serious sustainable environment-friendly agricultural enterprise with a great potential that improves export earnings. It is our fervent believe that the contribution of the apiculture sector to its sustainable human development and specifically increases the income of beekeepers and traders of bee products through improved commercialization of quality bee products in Nigeria which will in turn lead to a drastic increase in the GDP of the nation. The overall goal of the Nigeria Apiculture Value Chain (AVC) Development Program is to contribute to an increase income of beekeepers and other actors on the AVC through increase in production capacity, stimulate the efficiency and competitiveness of the AVC products by improving standards, processing capacity so as to access higher value niche markets through Fairtrade International (FLO) certification or organic certifications and to achieve a total sales value of over $5 million by 2035 (Sustainable Development Goal SDG) target on Food Security. Apiculture provides highly desirable products such as honey, comb/wax, pollen, propolis, bee venom, royal jelly, apilarnil and apilanil prop, value added products and pollination services. It is of note to stress the need to urgently embrace the use of modern techniques in keeping honeybees for industrial development of Nigeria and also to enhance exportation of the products to earn growth in the Nigeria’s GDP by diversifying the monolithic economy that is presently based on crude oil exportation.
尼日利亚目前的经济挑战要求建立一个具有巨大潜力的可持续、环境友好的农业企业,以提高出口收入。我们坚信,养蜂业对其可持续人类发展的贡献,特别是通过改善尼日利亚优质蜂产品的商业化,增加养蜂人和蜂产品贸易商的收入,这反过来将导致该国GDP的大幅增长。尼日利亚养蜂价值链发展计划的总体目标是通过提高生产能力来增加养蜂人和养蜂价值链上其他参与者的收入,通过提高标准来刺激养蜂价值链产品的效率和竞争力,加工能力,以便通过国际公平贸易(FLO)认证或有机认证进入价值更高的利基市场,并在2035年实现粮食安全总销售额超过500万美元的目标(可持续发展目标SDG)。养蜂业提供非常理想的产品,如蜂蜜、梳子/蜡、花粉、蜂胶、蜂毒、蜂王浆、蜂蜡和蜂蜡道具、增值产品和授粉服务。值得注意的是,需要强调的是,迫切需要利用现代技术饲养蜜蜂,以促进尼日利亚的工业发展,并通过使目前以原油出口为基础的单一经济多样化,加强产品出口,以赚取尼日利亚国内生产总值的增长。
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引用次数: 0
Wheat Yield in a Tolerant Winter Wheat Line Infested by Hessian Fly (Mayetiola destructor) 黑森蝇侵染耐受性冬小麦品系小麦产量分析
Pub Date : 2021-03-01 DOI: 10.4236/AE.2021.92007
K. E. Roe, B. Schemerhorn
The Hessian fly, Mayetiola destructor, is a harmful pest of wheat (Triticum aestivum). Pioneer variety 25R78 is putatively tolerant, meaning that the plant can survive successful Hessian fly infestation with reduced growth effects. To understand if Hessian fly-tolerance in wheat results in reduced yield effects and to analyze the economic feasibility of tolerant wheat as a Hessian fly control method, this study focused on analyzing the effect of infestation on tolerant wheat yield. This study analyzed tolerant Pioneer variety 25R78, resistant Pioneer brand variety 25R32, and susceptible Pioneer brand variety 25R47 through harvest. Treated plants were infested using a plastic cover and allowing 1 - 2 female flies to lay eggs for two hours. We measured head, fertile head number and tiller number. Seeds were analyzed by measuring total seed number and weight, as well as average seed number and weight. Tolerant and resistant plants showed no significant effects on yield in comparison to susceptible wheat. The infested tolerant plants were comparable in yield to infested resistant plants. Therefore, we propose that tolerance incorporated into wheat varieties will lower selection pressure on Hessian fly populations and increase the durability of these wheat lines.
黑森蝇是小麦的有害害虫。先锋品种25R78被认为是耐受性的,这意味着该植物可以在成功的黑森蝇侵扰中存活下来,同时降低生长效果。为了了解小麦对黑森蝇的耐受性是否会导致产量下降,并分析耐受小麦作为黑森蝇防治方法的经济可行性,本研究重点分析了虫害对耐受小麦产量的影响。本研究通过收获分析了耐先锋品种25R78、耐先锋品牌品种25R32和易感先锋品牌品种25 R47。处理过的植物用塑料罩感染,让1-2只雌蝇产卵两个小时。我们测量了头数、可育头数和分蘖数。通过测量种子总数和重量以及平均种子数和重量来分析种子。与感病小麦相比,耐受和抗性植物对产量没有显著影响。受感染的耐受植物在产量上与受感染的抗性植物相当。因此,我们提出,将耐受性纳入小麦品种将降低黑森蝇种群的选择压力,并提高这些小麦品系的耐久性。
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引用次数: 0
Trials for Gathering Information on an Unknown Peak in the GC-MS Spectra of Horse and Pony Hair Extracts 收集马和马鬃提取物GC-MS光谱中未知峰信息的试验
Pub Date : 2021-03-01 DOI: 10.4236/AE.2021.92009
M. Tsikolia, T. Opatz, U. Kauhl, N. Tabanca, B. Demirci, S. TenBroeck, K. Linthicum, U. Bernier
The volatile compounds from horse and pony hairs and skin were analyzed to determine bioactive molecules that are kairomones used for host location by blood-feeding diptera. In this study, horse and pony hair samples were extracted with organic solvents (pentane or hexane) and analyzed by gas chromatography-mass spectrometry analysis (GC-MS) with closed electron ionization (CEI). Using the GC-MS analysis, we observed a compound peak, which could not be identified by comparison to mass spectra in the mass spectral library. This unknown compound was often the largest peak in the chromatogram and had a retention index and mass spectral fragmentation similar to nonanal. However, there were several differences in the fragmentation pattern. When compared to a nonanal reference standard, it was evident that this was a different compound. Hydrodistillation of pony hair was another extraction method to obtain the unknown component in higher concentrations. Analysis of this extract with GC-flame ionization detector (FID) with GC-MS confirmed the same unknown peak. Further experiments and analysis with the various mass spectroscopy tools gave the possible molecular ion with mass-to-charge ratio (m/z) 142, corresponding to the unknown component.
通过对马、小马毛发和皮肤中挥发性化合物的分析,确定了食血双翅目昆虫用于定位寄主的生物活性分子。本研究采用有机溶剂(戊烷或己烷)提取马毛样品,并采用封闭电子电离(CEI)气相色谱-质谱分析(GC-MS)进行分析。通过GC-MS分析,我们观察到一个复合峰,该峰无法通过与质谱库中的质谱进行比较来识别。这种未知的化合物通常是色谱中最大的峰,其保留指数和质谱碎片类似于壬醛。然而,在破碎化模式上存在一些差异。当与非肛门参考标准比较时,很明显这是一种不同的化合物。马鬃加氢蒸馏法是另一种获得高浓度未知成分的提取方法。用气相色谱-火焰离子化检测器(FID)和气相色谱-质谱联用分析证实了相同的未知峰。用各种质谱工具进行进一步的实验和分析,得出了可能的分子离子,其质量电荷比(m/z)为142,与未知成分相对应。
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引用次数: 2
期刊
昆虫学(英文)
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