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Sensory Characteristics, Nutritional Composition, and Quality of Eggs from Different Chickens 不同鸡产蛋的感官特性、营养成分和品质
Pub Date : 2022-01-01 DOI: 10.4236/ojas.2022.124043
Atsbaha Hailemariam, W. Esatu, S. Abegaz, M. Urge, G. Assefa, T. Dessie
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引用次数: 2
Assessment of Lactational Performance in Dairy Cows Receiving a Rumen Protected B Vitamin Blend during Lactation: Part 1: A Meta-Analysis of 50 Studies 泌乳期间接受瘤胃保护的B族维生素混合物的奶牛泌乳性能评估:第一部分:50项研究的荟萃分析
Pub Date : 2021-08-24 DOI: 10.4236/ojas.2021.114039
E. Evans, H. Leclerc, Ousama Al Zahal, E. Santin
The objective of this research was to evaluate the impact of a dietary supplemental rumen protected B vitamin blend (RPBV: Folic acid, B12, pyridoxine, pantothenic acid, and biotin; Jefo, St. Hyacinthe, QC Canada) on milk, fat, and protein yield in cows past peak lactation. Results from 50 double reversal design trials (control-test-control) were evaluated using meta-analyses. All herds participated in monthly milk recording services, and all were Holstein herds. Within each of the 50 trials, cows included in the statistical analysis were over 45 days in milk (DIM) at the first milk test, averaged 159 ± 26 DIM on the first test date, and were available for all milk collection periods. A total of 6483 cows from 7 countries were represented in the 50 studies, with an average herd size of 129.7 ± 118.0. Meta-analyses were conducted to determine effect size for milk, fat, and protein yield based on the treatment differences and standard errors (SE) from the individual trials. Results indicated that dairy cows responded positively to the inclusion of the rumen protected B vitamin blend. Forest plots revealed trial-to-trial variation and might be related to age, level of milk production, and stage of lactation of the cows.
本研究的目的是评估饲粮中添加保护瘤胃的维生素B混合物(RPBV:叶酸、B12、吡哆醇、泛酸和生物素)的影响;Jefo, St. hyacins, QC Canada)对泌乳高峰后奶牛的牛奶、脂肪和蛋白质产量的影响。采用meta分析对50项双逆转设计试验(对照-试验-对照)的结果进行评价。所有奶牛都参加了每月的牛奶记录服务,所有奶牛都是荷斯坦奶牛。在50个试验中,统计分析的奶牛在第一次试乳时的泌乳天数均在45 d以上,在第一次试乳日的平均泌乳天数为159±26 DIM,并且在所有采乳期均可用。50项研究共涉及来自7个国家的6483头奶牛,平均牛群规模为129.7±118.0头。进行荟萃分析,根据单个试验的处理差异和标准误差(SE)确定乳、脂肪和蛋白质产量的效应大小。结果表明,奶牛对添加保护瘤胃的B族维生素混合物反应良好。森林样地显示出试验间的差异,可能与奶牛的年龄、产奶量水平和泌乳阶段有关。
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引用次数: 1
Effects of Different Levels of Fertilization with Hen Droppings on the Production and Chemical Composition of Pennisetum clandestinum (Poaceae) 母鸡不同施肥水平对狼尾草产量及化学成分的影响
Pub Date : 2021-08-24 DOI: 10.4236/ojas.2021.114037
Wauffo David Fokom, F. Tendonkeng, Gilles Jiope Azangue, E. Miégoué, France-Gina Tobou Djoumessi, Nyah Cédric Kwayep, M. Mouchili
The use of organic manures, constitute an alternative to that of polluting artificial fertilizers. In this study, the effect of various levels of hen droppings fertilization on the production of biomass, chemical composition and carrying capacity of P. clandestinum were evaluated at the Research and Experimental Farm (REF) and at the Animal Nutrition and Feeding Laboratory of the University of Dschang, between April and September 2016. A completely randomized block design comparing three nitrogen level of fertilization in hen droppings form (0; 100 and 200 kg N/ha) on 4 m2 plot of P. clandestinum were used. Each treatment was carried out in four replications for a total of 12 experimental plots. Then a sample of 500 g of forage has been collected in the center of each plot, then dried and used for the evaluation of the biomass and the chemical composition of P. clandestinum, according to the level of fertilization and the cutting frequency. The results showed that the fertilization with the hen droppings positively influenced (p tion at the first cutting frequency and the highest value (47.12 cm) was obtained with of 200 kg N/ha rate of fertilization. The biomass of this fodder increased significantly (p s with the increasing cutting of frequency whatever the level of fertilization. Independently of the fertilization and the cutting frequency, the maximum biomass (6.22 t DM/ha) was obtained with the first cutting at the 200 kg N/ha of hen droppings fertilization. The fertilization influenced in variable way the chemical composition of P. clandestinum. The dry matter and organic matter content decrease with the level of fertilization and the highest values (83.79% and 89.39% respectively for the DM and OM) were obtained on the control plot at the third and the first cutting. Moreover, the ash and crude protein contents increased significantly (p
有机肥料的使用构成了污染人工肥料的替代品。在这项研究中,2016年4月至9月,在研究与实验农场(REF)和德昌大学动物营养与饲养实验室评估了不同水平的母鸡粪便施肥对隐尾蠊生物量、化学成分和携带能力的影响。采用完全随机区组设计,比较了在4平方米的P.clandestinum地块上以母鸡粪便形式(0、100和200公斤N/ha)施肥的三种氮水平。每种处理在总共12个实验区的四个重复中进行。然后,在每个地块的中心采集500克饲料样本,然后进行干燥,根据施肥水平和切割频率,用于评估隐尾蠊的生物量和化学成分。结果表明,用母鸡粪便施肥对第一次扦插频率有积极影响,在200 kg N/ha的施肥率下获得最高值(47.12 cm)。无论施肥水平如何,该饲料的生物量都随扦插频率的增加而显著增加(p<0.01)。与施肥和扦插频率无关,在200 kg N/ha的母鸡粪便施肥条件下,第一次扦插的生物量最高(6.22 t DM/ha)。施肥对隐尾藻的化学成分有不同程度的影响。干物质和有机质含量随着施肥水平的降低而降低,在第三次和第一次切割时,对照地块的干物质和无机质含量分别达到83.79%和89.39%。灰分和粗蛋白含量显著增加(p
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引用次数: 2
Genetic Diversity and Population Structure of Three Strains of Indigenous Tswana Chickens and Commercial Broiler Using Single Nucleotide Polymormophic (SNP) Markers 利用单核苷酸多态性(SNP)标记分析3个地方茨瓦纳鸡和商品肉鸡品系的遗传多样性和群体结构
Pub Date : 2021-08-24 DOI: 10.4236/ojas.2021.114035
J. Machete, P. Kgwatalala, S. J. Nsoso, N. Hlongwane, J. Morêki
The Tswana chicken is native to Botswana and comprises strains such as the naked neck, normal, dwarf, frizzled, and rumples. The origins of the different strains of Tswana chicken remain unknown and it is not yet clear if the different strains represent distinct breeds within the large Tswana chicken population. Genetic characterization of different strains of Tswana chickens using SNP arrays can elucidate their genetic relationships and ascertain if the strains represent distinct breeds of Tswana chicken population. The aim of this study was therefore to investigate population structure and diversity and to estimate genetic distances/identity between the naked neck, normal and dwarf strains of Tswana chickens. A total of 96 chickens (normal strain (n = 39), naked neck strain (n = 32), dwarf strain (n = 13) and commercial broiler (n = 12)) were used in the study. SNP genotyping was carried out using the Illumina chicken iSelect SNP 60 Bead chip using the Infinium assay compatible with the Illumina HiScan SQ genotyping platform. The observed heterozygosity (Ho) values were 0.610 ± 0.012, 0.611 ± 0.014, 0.613 ± 0.0006 for normal, naked neck and dwarf strains of Tswana chickens respectively and averaged 0.611 ± 0.016 across the three strains of Tswana chickens compared to Ho of 0.347 ± 0.023 in commercial broiler chicken. The expected heterozygosity (He) values were 0.613 ± 0.00012, 0.614 ± 0.00013, 0.608 ± 0.00021 for normal, naked neck and dwarf strains of Tswana chickens respectively and averaged 0.612 ± 0.00015 across the three strains of Tswana chickens compared to He of 0.577 ± 0.00022 in commercial broiler chicken. Principal component analysis (PCA) was used to get an insight into the population structure of indigenous Tswana chickens. The first two principal components revealed a set of three clusters. The normal strain of Tswana chicken and commercial broiler clustered together in one group. The dwarf strain clustered separately in one group and the naked neck and normal strains clustered together in the last group. The separate clustering of the dwarf strain from the rest of Tswana chicken strains suggests significant genetic uniqueness of the dwarf strain and very close genetic similarities between the normal and naked neck strains. The clustering pattern was confirmed by less genetic differentiation and less genetic distances between the naked neck and normal strains of Tswana chicken than between the two strains and the dwarf strain of Tswana chicken.
茨瓦纳鸡原产于博茨瓦纳,包括裸颈鸡、普通鸡、侏儒鸡、卷曲鸡和皱褶鸡等品种。茨瓦纳鸡不同品系的起源仍然未知,目前尚不清楚这些不同品系是否代表了茨瓦纳鸡大种群中的不同品种。利用SNP阵列对不同茨瓦纳鸡品系进行遗传鉴定,可以阐明它们的遗传关系,并确定这些品系是否代表茨瓦纳鸡种群的不同品种。因此,本研究的目的是调查茨瓦纳鸡裸颈、正常和矮株的种群结构和多样性,并估计遗传距离/同一性。试验共选用96只鸡(正常品系39只,裸颈品系32只,矮化品系13只,商品肉鸡12只)。采用与Illumina HiScan SQ基因分型平台兼容的Infinium检测方法,使用Illumina鸡iSelect SNP 60 Bead芯片进行SNP基因分型。正常株、裸颈株和矮株茨瓦纳鸡的杂合度(Ho)分别为0.610±0.012、0.611±0.014和0.613±0.0006,平均为0.611±0.016,而商品肉鸡的Ho为0.347±0.023。正常株、裸颈株和矮株茨瓦纳鸡的期望杂合度(He)分别为0.613±0.00012、0.614±0.00013和0.608±0.00021,平均为0.612±0.00015,而商品肉鸡的He为0.577±0.00022。采用主成分分析(PCA)对土着茨瓦纳鸡种群结构进行了分析。前两个主要成分揭示了一组三个集群。正常菌株的茨瓦纳鸡和商品肉鸡聚集在一组。矮株单独聚集在一组,裸颈株和正常株聚集在最后一组。矮株与其他茨瓦纳鸡菌株的分离聚类表明矮株具有显著的遗传独特性,而正常株与裸颈株的遗传相似性非常接近。茨瓦纳鸡裸颈株与正常株之间的遗传分化较小,遗传距离也小于两株与茨瓦纳鸡矮株之间的遗传距离。
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引用次数: 0
Exploratory Study on Relationship among Body Weight, Body Condition Score and Some Blood Biochemical Parameters of Non-Descriptive Goats in Mzimvubu Local Municipality: A Case of Santombe Village Mzimvubu市非描述性山羊体重、身体状况评分与某些血液生化参数关系的探讨——以Santombe村为例
Pub Date : 2021-08-24 DOI: 10.4236/ojas.2021.114044
S. Mthi, N. Nyangiwe, F. R. Gwaze, M. Yawa, Thobela Louis Tyasi, S. Tokozwayo, T. Thubela, M. Jansen, S. Goni, T. Khetani, L. Qokweni, S. Washaya, Busiswa Guza, Mlungisi Magwaza, Bekikaya Brilliant Mbangi, Thandile Ndobeni
Body weight is a crucial trait that aids farmers in decision-making regarding vaccinations, feeding, marketing and selection during the breeding programs. The study was conducted to investigate the effect of sex and age on body weight (BW), body condition score (BCS) and some blood biochemical parameters (BBP) and to determine the correlation amongst BW, BCS and BBP of non-descriptive goats at Santombe communal farming system. Blood samples were collected from 33 apparently healthy goats (27 females and 6 males) aged 6 - 8 tooth with a mean BW (32.62 ± 11.39 kg) while BCS (3.17 ± 0.39) was taken by means of palpating the lumbar area, sternum and the ribs. The BCS was rated on a scale of 1 to 5 with 1 being emaciated and 5 being extremely fat. The blood samples were analyzed using Cobas intergra 400 plus chemistry analyzer, Roche for biochemical parameters such as total protein (TP), urea, cholesterol (Chol), magnesium (Mg), phosphorous (P) and calcium (Ca) with mean values of 72.79 ± 5.84 mg/l, 2.76 ± 1.16 mmol/l, 2.08 ± 0.40 mmol/l, 1.09 ± 0.09 mmol/l, 2.30 ± 0.49 mmol/l and 2.45 ± 0.12 mmol/l respectively. Sex and age had significant effects on BW, BCS and some biochemical parameters. The BW and BCS for males were significantly higher than for females. Correlation results indicated that BW had a positive relationship with BCS (r = 0.677), TP and BW (r = 0.400), Chol and BCS (r = 0.4025) and Mg and TP (r = -0.075). Age and sex had significant influences on BW, BCS and some blood biochemical parameters. Consideration of the factors can aid in determining the accurate diagnosis of the nutritional status of goats.
体重是一个关键特征,有助于农民在育种计划中就疫苗接种、喂养、营销和选择做出决策。本研究旨在调查性别和年龄对Santombe社区养殖系统中非描述性山羊体重(BW)、身体状况评分(BCS)和某些血液生化参数(BBP)的影响,并确定其体重、BCS和BBP之间的相关性。从33只6-8岁的健康山羊(27只雌性和6只雄性)身上采集血样,平均体重(32.62±11.39kg),而BCS(3.17±0.39)是通过触诊腰部、胸骨和肋骨来采集的。BCS的评分为1-5分,其中1分为消瘦,5分为极度肥胖。使用罗氏Cobas integral 400 plus化学分析仪对血液样本进行生化参数分析,如总蛋白(TP)、尿素、胆固醇(Chol)、镁(Mg)、磷(P)和钙(Ca),平均值分别为72.79±5.84 Mg/l、2.76±1.16 mmol/l、2.08±0.40 mmol/l、1.09±0.09 mmol/l、2.30±0.49 mmol/l和2.45±0.12 mmol/l。性别和年龄对体重、BCS及某些生化指标有显著影响。雄性的BW和BCS显著高于雌性。相关结果表明,BW与BCS(r=0.677)、TP与BW(r=0.400)、Chol与BCS、Mg与TP(r=-0.075)呈正相关,年龄和性别对BW、BCS及某些血液生化指标有显著影响。考虑这些因素可以帮助确定山羊营养状况的准确诊断。
{"title":"Exploratory Study on Relationship among Body Weight, Body Condition Score and Some Blood Biochemical Parameters of Non-Descriptive Goats in Mzimvubu Local Municipality: A Case of Santombe Village","authors":"S. Mthi, N. Nyangiwe, F. R. Gwaze, M. Yawa, Thobela Louis Tyasi, S. Tokozwayo, T. Thubela, M. Jansen, S. Goni, T. Khetani, L. Qokweni, S. Washaya, Busiswa Guza, Mlungisi Magwaza, Bekikaya Brilliant Mbangi, Thandile Ndobeni","doi":"10.4236/ojas.2021.114044","DOIUrl":"https://doi.org/10.4236/ojas.2021.114044","url":null,"abstract":"Body weight is a crucial trait that aids farmers in \u0000decision-making regarding vaccinations, feeding, marketing and selection during \u0000the breeding programs. The study was conducted to investigate the effect of sex \u0000and age on body weight (BW), body condition score (BCS) and some blood \u0000biochemical parameters (BBP) and to determine the correlation amongst BW, BCS \u0000and BBP of non-descriptive goats at Santombe communal farming system. Blood \u0000samples were collected from 33 apparently healthy goats (27 females and 6 males) \u0000aged 6 - 8 tooth with a mean BW (32.62 ± 11.39 kg) while BCS (3.17 ± 0.39) was \u0000taken by means of palpating the lumbar area, sternum and the ribs. The BCS was \u0000rated on a scale of 1 to 5 with 1 being emaciated and 5 being extremely fat. \u0000The blood samples were analyzed using Cobas intergra 400 plus chemistry \u0000analyzer, Roche for biochemical parameters such as total protein (TP), urea, \u0000cholesterol (Chol), magnesium (Mg), phosphorous (P) and calcium (Ca) with mean \u0000values of 72.79 ± 5.84 mg/l, 2.76 ± 1.16 mmol/l, 2.08 ± 0.40 mmol/l, 1.09 ± \u00000.09 mmol/l, 2.30 ± 0.49 mmol/l and 2.45 ± 0.12 mmol/l respectively. Sex and \u0000age had significant effects on BW, BCS and some biochemical parameters. The BW \u0000and BCS for males were significantly higher than for females. Correlation \u0000results indicated that BW had a positive relationship with BCS (r = 0.677), TP \u0000and BW (r = 0.400), Chol and BCS (r = 0.4025) and Mg and TP (r = -0.075). Age \u0000and sex had significant influences on BW, BCS and some blood biochemical \u0000parameters. Consideration of the factors can aid in determining the accurate \u0000diagnosis of the nutritional status of goats.","PeriodicalId":62784,"journal":{"name":"动物科学期刊(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49434994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Quantitative Feed Restriction Duration on Growth Performance and Carcass Characteristics of Broiler Chickens 定量限饲期对肉仔鸡生长性能和胴体特性的影响
Pub Date : 2021-08-24 DOI: 10.4236/ojas.2021.114043
Ziphozihle Gobane, S. Goni, D. Chikwanda, Leocadia Zhou
The growth productivity of broilers is influenced by improving growth performance through improving body weight, average daily gain, and feed conversion rate. The objective of the study was to determine the effect of quantitative feed restriction duration on growth performance and carcass characteristics of broilers. A total of 144 day-old broiler chicks were reared in a deep litter system until slaughter at 42 days. Chicks were randomly allocated to three treatments which were replicated three times with 16 birds per replicate. The three treatments were the control (T1), one week of feed restriction (T2), and two weeks of feed restriction (T3). Birds were fed with starter, grower and finisher diets. The average daily gain (ADG), average daily feed intake (ADFI), and the feed conversion ratio (FCR) were computed for each week. Carcass weight and internal organs weights were measured. The body weight gained by the birds in T1 was similar to the birds that were restricted for a week. The treatments had a significant effect on the average daily gain in weeks 4, 5, and 6. The ADG for T2 was higher (P ad libitum at week 4 at the start of the treatment. There was no significant effect on the relative weights of heart weights, liver, gizzard, feet, and heads among the treatment groups except for intestine weight which was lower (P
肉鸡的生长生产力受通过提高体重、平均日增重和饲料转化率来提高生长性能的影响。本研究的目的是确定定量限饲时间对肉鸡生长性能和胴体特性的影响。共有144天大的肉鸡在深窝系统中饲养,直到42天屠宰。鸡被随机分配到三个处理中,每个处理重复三次,每次重复16只。三个处理是对照(T1)、一周的饲料限制(T2)和两周的饲料控制(T3)。鸟类采用起始日粮、生长日粮和结束日粮喂养。每周计算平均日增重(ADG)、平均日采食量(ADFI)和饲料转化率(FCR)。测量胴体重量和内脏重量。T1期的鸟类体重增加与限制一周的鸟类相似。治疗对第4、5和6周的平均日增重有显著影响。T2的ADG在治疗开始的第4周时更高(P随意)。除了肠道重量较低外,治疗组的心重、肝重、胃重、脚重和头重的相对重量没有显著影响(P
{"title":"The Effect of Quantitative Feed Restriction Duration on Growth Performance and Carcass Characteristics of Broiler Chickens","authors":"Ziphozihle Gobane, S. Goni, D. Chikwanda, Leocadia Zhou","doi":"10.4236/ojas.2021.114043","DOIUrl":"https://doi.org/10.4236/ojas.2021.114043","url":null,"abstract":"The growth productivity of broilers is influenced by improving growth \u0000performance through improving body weight, average daily gain, and feed conversion rate. \u0000The objective of the study was to determine the effect of quantitative feed \u0000restriction duration on growth performance and carcass characteristics of \u0000broilers. A total of 144 day-old broiler chicks were reared in a deep litter \u0000system until slaughter at 42 days. Chicks were randomly allocated to three \u0000treatments which were replicated three times with 16 birds per replicate. The \u0000three treatments were the control (T1), one week of feed restriction (T2), and \u0000two weeks of feed restriction (T3). Birds were fed with starter, grower and \u0000finisher diets. The average daily gain (ADG), average daily feed intake (ADFI), and the feed conversion \u0000ratio (FCR) were computed for each week. Carcass weight and internal organs weights were measured. The body \u0000weight gained by the birds in T1 was similar to the birds that were restricted for a week. The \u0000treatments had a significant effect on the average daily gain in weeks 4, 5, and 6. The ADG for T2 was \u0000higher (P ad libitum at week 4 at the start of \u0000the treatment. There was no significant effect on the relative weights of heart \u0000weights, liver, gizzard, feet, and \u0000heads among the treatment groups except for intestine weight which was lower (P","PeriodicalId":62784,"journal":{"name":"动物科学期刊(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43535568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Morphometric Differentiation between Two Closely Related Achatinid Snails (Gastropoda: Achatinidae) of West Africa and Implications for the Conservation of Achatina togoensis (Bequaert & Clench, 1934) 西非两种亲缘关系较近的阿卡提亚门蜗牛(腹足纲:阿卡提亚科)的形态差异及其对togoensis的保护意义(Bequaert & Clench, 1934)
Pub Date : 2021-08-24 DOI: 10.4236/ojas.2021.114038
K. Afiademanyo, K. Awaga, Kamilou Ouro-Sama, Hodabalo Dheoulaba Solitoke
Achatina togoensis is a giant land snail taxa endemic to the northern sectors of the Monts Togo, which has been regarded as full species or subspecies of the highly variable, widespread A. achatina (L.). To address this issue, samples of snails were taken throughout the distribution range of the two taxa in the Dahomey Gap (West Africa) and separated into 112 A. achatina and 141 A. togoensis according to color of the columella and parietal wall. Radulae were compared and shell characters of these 2 species were evaluated through the traditional morphometric method. The results indicate that the species cannot be distinguished by overall shell size and radular morphology, but they exhibit significant differences in shell shape and meristic traits. The typical A. achatina with vinaceous-red columella has a low-spired shell and an expanded aperture (globose shape) whereas A. togoensis has a higher-spired and smaller aperture (a fusiform shape). We suggest that local environmental effects are probably the causes of morphological divergence between the two taxa. It is likely that peripheral populations of the typical form of A. achatina would have isolated and developed in the drier facies of the humid forest that individualized in the northern sector of the mountains after the arid or interpluvial period of Holocene. In addition, we find that A. togoensis meets the criteria B2ab (ii, iii) to categorize as “Endangered”. However, whether this latter should be considered as separate species cannot be decided at the current state of knowledge.
多哥阿卡蒂纳蜗牛是多哥北部特有的一种巨型陆地蜗牛类群,被认为是高度可变、广泛分布的阿卡蒂娜蜗牛(L.)的完整物种或亚种。为了解决这个问题,在达荷美峡(西非)两个分类群的分布范围内采集了蜗牛样本,并根据小柱和顶壁的颜色将其分为112个A.achatina和141个A.togoensis。采用传统形态计量学方法对这两个种的Radulae进行了比较,并对其外壳性状进行了评价。结果表明,该物种不能通过整体外壳大小和瓣状形态来区分,但它们在外壳形状和分生组织特征上表现出显著差异。典型的具有醋红色小柱的A.achatina有一个低尖顶的外壳和一个扩大的孔径(球形),而A.togoensis有一个更高的尖顶和更小的孔径(纺锤形)。我们认为,局部环境影响可能是两个分类群之间形态差异的原因。在全新世干旱或坡间期之后,典型的A.achatina外围种群很可能在潮湿森林的干燥相中分离和发展,而潮湿森林在山区北部个体化。此外,我们发现A.togoensis符合B2ab(ii,iii)标准,被归类为“濒危”。然而,后者是否应该被视为单独的物种,在目前的知识状态下还不能决定。
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引用次数: 2
Evaluation of the Hypothalamic Kisspeptin System throughout the Estrous Cycle in Gilts 在Gilts的整个发情周期中评估下丘脑Kisspeptin系统
Pub Date : 2021-08-24 DOI: 10.4236/ojas.2021.114040
J. Clapper, Eric Jolitz, W. Dhillo
Kisspeptin has been demonstrated to affect reproductive cyclicity and the attainment of puberty in multiple species, presumably through its actions on gonadotropin releasing hormone and luteinizing hormone. Kisspeptin administration causes increased plasma concentrations of LH in pigs, sheep, and rats. The objective of this experiment was to evaluate changes in the hypothalamic kisspeptin system throughout the estrous cycle in gilts. Estrus was synchronized in forty crossbred gilts (191 d, 121 kg) and estrus detection was performed by exposing gilts to a mature boar. The first day gilts stood immobile was denoted d 1 of the estrous cycle. Blood samples were collected via jugular venipuncture on d 1, 4, 7, 9, 14, 16, and 19 of the estrous cycle. Ten animals were slaughtered on d 1, 9, 14, and 21 of the estrous cycle when medial basal hypothalami, anterior pituitary glands, and blood were collected. Relative expression of hypothalamic kisspeptin (KISS1), kisspeptin receptor (KISS1R), estrogen receptors-a, anterior pituitary gland GnRH receptor, β-actin, and GAPDH was determined using real-time reverse transcriptase PCR. Fold changes in relative expression were determined using the Relative Expression Software Tool. Relative expression of KISS1 was increased (P = 0.006) 3.2 fold on d 1 versus d 21 and 2.3 fold (P = 0.003) on d 9 versus d 21 of the estrous cycle, but was not different (P > 0.05) among the remaining days of the estrous cycle. Relative expression of estrogen receptor-b was decreased (P = 0.05) 0.8 fold on d 9 versus d 21 and (P = 0.005) 0.7 fold on d 14 versus d 21, but was not different (P > 0.05) among the remaining days. Relative expression of anterior pituitary gland GnRH receptor was increased (P
Kisspeptin已被证明会影响多个物种的生殖周期和青春期的实现,可能是通过其对促性腺激素释放激素和黄体生成激素的作用。Kisspeptin给药导致猪、绵羊和大鼠血浆LH浓度升高。本实验的目的是评估在母猪发情周期中下丘脑kisspeptin系统的变化。在40头杂交母猪(191天,121公斤)中同步发情,并通过将母猪暴露于成熟公猪来进行发情检测。母猪静止不动的第一天表示为发情周期的第1天。在发情周期的第1、4、7、9、14、16和19天通过颈静脉穿刺采集血样。在发情周期的第1天、第9天、第14天和第21天屠宰10只动物,采集内侧基底下丘脑、垂体前叶和血液。使用实时逆转录聚合酶链式反应测定下丘脑kisspeptin(KISS1)、kisspepton受体(KISS1R)、雌激素受体-a、垂体前叶GnRH受体、β-肌动蛋白和GAPDH的相对表达。使用相对表达软件工具测定相对表达的折叠变化。KISS1的相对表达在发情周期的第1天比第21天增加了3.2倍(P=0.006),在第9天和第21天分别增加了2.3倍(P=0.003),但在发情周期剩余的几天之间没有差异(P>0.05)。雌激素受体-b的相对表达在第9天和第21天分别降低了0.8倍和0.7倍,但在其余天数之间没有差异(P>0.05)。垂体前叶GnRH受体的相对表达增加(P
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引用次数: 0
In Vitro Digestibility and Gas Production from E. crus-pavonis Used in Wetlands from Domestic Wastewater Treatment 应用于生活污水处理湿地的克氏绿脓杆菌体外消化率及产气量研究
Pub Date : 2021-08-24 DOI: 10.4236/OJAS.2021.114041
Gabriel Nicodème Tsetagho, Martin Lekeufack, M. Mouchili, E. Tedonkeng, Th ophile Fonkou
In order to evaluate the possibilities of valorization as the feed of the plant biomass produced during wastewater treatment in constructed wetlands, a study of the in vitro digestibility and gas production of Echinochloa crus-pavonis was carried out in the Laboratory of Animal Production and Nutrition of the University of Dschang. The in vitro digestibility of Echinochloa crus-pavonis was evaluated at different harvesting periods. The digestibility parameters of E. crus-pavonis samples were determined by the in vitro method at different phenological stages. The gas production (GP) during 24 h of incubation was assessed using the in vitro incubation technique with bovine rumen fluid. In vitro dry matter digestibility (IVDM) values ranged from 52.09% to 64.76% with a decrease observed with the phenological stages of E. crus-pavonis (from 64.76% at the leafy stage to 52.09% at the flowering stage). The microbial biomass (MB) values varied significantly between 67.99 and 88.45 mg, with no significant difference observed between the leafy (88.45 mg), bolting (82.93 mg), and early heading (80.26 mg) stages (P > 0.05). On the other hand, changes in the gas produced during 24 h from the studied samples of E. crus-pavonis (34.9 and 48 ml/500mg) and volatile fatty acids (VFA) values (1.08 and 0.80 mmol/ml) decreased significantly (P E. crus-pavonis in rumen fluid significantly decreased with advanced plant maturity (P E. crus-pavonis. By combining the requirements of an optimal quantitative and qualitative production of E. crus-pavonis, harvesting at the bolting or early heading stage is an option of choice for exploitation as forage, under the conditions of this study. Based on the in vitro digestibility parameters studied, E. crus-pavonis is suitable as a ruminant feed.
为了评估人工湿地废水处理过程中产生的植物生物量作为饲料的价值化可能性,在德昌大学动物生产与营养实验室对刺针草的体外消化率和产气量进行了研究。在不同收获期对帕冯刺针草的体外消化率进行了评价。采用体外法测定了不同酚期巴豆样品的消化率参数。使用牛瘤胃液体外孵育技术评估孵育24小时期间的产气量(GP)。体外干物质消化率(IVDM)值在52.09%-64.76%之间,随着巴沃尼香茅的酚期而降低(从叶期的64.76%降低到开花期的52.09%)。微生物生物量(MB)值在67.99和88.45mg之间变化显著,叶期(88.45mg)、抽薹期(82.93mg)和早穗期(80.26mg)之间没有显著差异(P>0.05),在24小时内,所研究的帕沃尼香豆样品(34.9和48 ml/500mg)产生的气体和挥发性脂肪酸(VFA)值(1.08和0.80 mmol/ml)的变化显著降低(随着植物成熟度的提高,瘤胃液中的帕沃尼斯香豆显著降低(P.crus pavonis。通过结合最佳数量和质量生产的要求,在本研究的条件下,在抽薹或抽穗早期收获是作为饲料开发的一种选择。根据研究的体外消化率参数,E.crus pavanis适合作为反刍动物饲料。
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引用次数: 0
Testing Toxicity and Antidote Effect of Selenium Nanoparticles with Paramecium caudatum 硒纳米粒子对草履虫的毒性及解药作用
Pub Date : 2021-08-24 DOI: 10.4236/ojas.2021.114036
Khandsuren Badgar, J. Prokisch
A simple method for assessment of the toxicity and antidote effect of selenium nanoparticles with Paramecium caudatum is presented. Light microscopy in combination with computerized video tracking is employed for the determination of the survival time of P. caudatum. Up to 800 mg/L, selenium nanoparticles are not acutely toxic. With respect to a potential antidote effect, the lethality of silver nanoparticles, silver nitrate, sodium hydrogen selenite, and sodium selenite to P. caudatum was decreased and survival time was extended upon pre-treatment with selenium nanoparticles. Taken together, these findings suggest that administration of selenium nanoparticles attenuates exposure to toxicants. Selenium nanoparticles could be a good functional additive for food management in animals.
提出了一种简单的方法来评估纳米硒对草履虫的毒性和解毒作用。采用光学显微镜结合计算机视频跟踪来测定尾状藻的生存时间。高达800 mg/L的硒纳米颗粒没有急性毒性。关于潜在的解药作用,在用硒纳米颗粒预处理后,银纳米颗粒、硝酸银、亚硒酸氢钠和亚硒酸钠对尾状藻的致死性降低,存活时间延长。总之,这些发现表明,硒纳米颗粒的给药可以减少对有毒物质的暴露。硒纳米粒子可能是一种很好的动物食品管理功能添加剂。
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引用次数: 2
期刊
动物科学期刊(英文)
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