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2010 4th International Conference on Bioinformatics and Biomedical Engineering最新文献

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Ozone-Induced Changes in Antioxidant Systems of Ginkgo Biloba in Relation to the Developmental Stage of the Leaves 臭氧诱导银杏叶片抗氧化系统与发育阶段的关系
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516255
Kun Yan, Xingyuan He, Wei Chen, Tao Lu
Ozone (O3) pollution has adverse effects on the trees grown in urban area. Using open top chambers, responses of antioxidant systems were detected in Ginkgo biloba leaves at different developmental stages after a long term O3 exposure (O3 concentration ≈ 80 nmol mol-1). As leaf size is closely related to the developmental stage, leaves with different sizes were chosen to indicate the various developmental stages. The results suggested that activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) were induced to a higher level in younger leaves with 50% expansion size under elevated O3 exposure, whereas O3-induced decrease in APX and SOD activities was found in the 80% and 100% expanded ones. Dehydroascorbate reductase (DHAR) activity and ascorbate (Asc) content were not affected by high O3 in 50% expanded leaves, but the O3-induced decrease in them occurred in the 80% and 100% expanded ones. Malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents increased significantly in the 80% and 100% expanded leaves at elevated O3. However, O3-induced increase in MDA and H2O2 contents did not reach the significant level in the 50% expanded leaves, indicating the higher resistance to O3 in the younger leaves. The positive responses of antioxidant systems could be responsible for the higher resistance to O3 in the younger leaves.
臭氧(O3)污染对城市树木生长有不利影响。采用开顶实验室,研究了长期暴露于O3 (O3浓度≈80 nmol mol-1)下不同发育阶段银杏叶片抗氧化系统的反应。由于叶片大小与发育阶段密切相关,因此选择不同大小的叶片来表示不同的发育阶段。结果表明,在O3浓度升高的情况下,膨大50%的幼叶中超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)和过氧化氢酶(CAT)活性升高,而膨大80%和100%的幼叶中APX和SOD活性降低。50%展开叶中脱氢抗坏血酸还原酶(DHAR)活性和抗坏血酸(Asc)含量不受高氧的影响,而80%和100%展开叶中脱氢抗坏血酸还原酶(DHAR)活性和抗坏血酸(Asc)含量受到高氧的影响。高浓度O3处理下,80%和100%膨大叶片丙二醛(MDA)和过氧化氢(H2O2)含量显著增加。而O3诱导的MDA和H2O2含量的增加在50%膨大叶片中未达到显著水平,说明幼龄叶片对O3的抗性较高。抗氧化系统的积极响应可能是幼龄叶片对O3抗性较高的原因。
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引用次数: 0
Identification of a Novel S-RNase Gene and S-Genotypes of Four Pear (Pyrus pyrifolia) Cultivars 4个梨品种S-RNase新基因及其s基因型的鉴定
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516677
Lin Zhang, Jiao Hu, X. Tan, Hongxu Long, D. Yuan, Xiu-gen Li
Gametophytic self-incompatibility (GSI) is a genetically controlled mechanism to prevent inbreeding and promote out-crossing. Pyrus pyrifolia is a commercially important fruit tree that exhibits GSI. It is necessary to identify S-genotypes of cultivars for determination of cross-compatible combination prior to performing pear plantation and breeding programs. In this study, an important local cultivar 'Huobali' extensively used as parent in pear breeding programs, and its three progenies 'Mantianhong', 'Hongsucui' and 'Meirensu' were used for S-genotyping analysis by PCR-based molecular method. Agarose gel electrophoresis showed that two fragments were produced in each of the three progenies, while three in the 'Huobali'. After restriction digestion and sequencing analysis, the S-alleles in the four cultivars were determined. The allele of 345 bp from 'Huobali' was determined as a novel S-RNase allele that was tentatively denominated as S44-RNase. RT-PCR revealed that the S44-RNase was expressed specifically in the styles, which is consistent with the expression pattern of S-RNases. Comparison of genomic and cDNA sequences revealed an intron of 148 bp for the S44-RNase. At the amino acid level, it shared 65% to 93% similarity with other Maloideae S-RNases. This study will be helpful in pear production and breeding programs.
配子体自交不亲和(GSI)是一种防止近交和促进异交的遗传控制机制。梨叶是一种商业上重要的果树,具有GSI。在实施梨树种植和育种计划之前,有必要对品种进行s基因型鉴定,以确定杂交亲和性组合。本研究以在梨育种中广泛应用的重要地方栽培品种‘火巴梨’及其3个子代‘满天红’、‘红素萃’和‘美仁素’为材料,采用基于pcr的分子分型方法进行了s基因分型分析。琼脂糖凝胶电泳结果显示,3个子代各产生2个片段,而“火八”子代产生3个片段。经酶切和测序分析,确定了4个品种的s等位基因。从“火巴里”获得的345 bp等位基因被确定为一个新的S-RNase等位基因,暂定为S44-RNase。RT-PCR结果显示,S44-RNase在花型中特异表达,与s - rnase的表达模式一致。基因组序列和cDNA序列比较显示S44-RNase的内含子长度为148 bp。在氨基酸水平上,它与其他马洛亚科S-RNases的相似性为65% ~ 93%。本研究对梨的生产和育种具有一定的指导意义。
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引用次数: 0
Chemical Composition and Possible Sources of Elements in Dustfall in Pingdingshan City 平顶山市降尘中元素的化学组成及可能来源
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516593
Zhangxian Liu, Yan Zhang, Lianshan Li, R. Guo, K. Huang
Dustfall samples were synchronously collected at five typical urban functional areas (industrial area, traffic area, residential area, cultural and educational area, residential and commercial mixed area) of Pingdingshan City in Henan Province from May 2008 to April 2009. The principal component elements in dustfall samples were analyzed for concentrations, spatial distribution, sources and contributions to air pollution. The results indicated that Cd, Pb, As, Zn, S and Cu were the dominant polluted elements in the dustfall and their enrichment factors were 44.1, 15.0, 7.8, 3.4, 3.4 and 4.4, respectively. The major ionic species in dustfall were Ca2+, SO42-, Cl-, K+, Na+, F- and NO2-, and their concentrations were 2112, 825, 193, 177, 136, 136 and 130µgg-1, respectively. The industrial dust and surface soil dust, coal burning dust and constructing dust, motor vehicle emission and metal smelting dust, oil burning and waste incineration should be the dominant sources of dustfull and their contribution were 38.68%, 25.78%, 19.57%, 10.98%, respectively. Therefore, it obviously indicated that the atmospheric dust full was the multi-source pollution in Pingdingshan.
2008年5月至2009年4月,在河南省平顶山市5个典型城市功能区(工业区、交通区、居住区、文教区、商住混合区)同步采集降尘样本。分析了降尘样品中主成分元素的浓度、空间分布、来源和对大气污染的贡献。结果表明,Cd、Pb、As、Zn、S和Cu是主要污染元素,富集系数分别为44.1、15.0、7.8、3.4、3.4和4.4。降尘离子主要为Ca2+、SO42-、Cl-、K+、Na+、F-和NO2-,浓度分别为2112、825、193、177、136、136和130µgg-1。工业扬尘和地表土壤扬尘、燃煤扬尘和建筑扬尘、机动车排放扬尘和金属冶炼扬尘、燃油燃烧扬尘和垃圾焚烧扬尘是主要的扬尘来源,贡献率分别为38.68%、25.78%、19.57%和10.98%。由此可见,平顶山大气粉尘是多源污染。
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引用次数: 1
Maturity Evaluation of Spent Mushroom Compost 废蘑菇堆肥的成熟度评价
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5517138
Yaning Luan, Xiangyang Sun, Suyan Li, Yong Qiao, L. Suo
Maturity of compost is an important practical factor in agricultural and horticulture production. This study was conducted to perform maturity assessment for spent mushroom compost (SMC). Compost maturity were evaluated using standard chemical analyses (pH, EC, CEC, total C and N content, C/N ratio, NH4+-N, NO3--N). Subsequently, potential phytotoxicity from SMC was assessed by germination bioassay using Lepidium sativum L. (cress). SMC samples were collected periodically during a 28-d rapid composting period from a single windrow. Following composting time, the pH and EC values had a tendency to increase, and in the end, the two values maintained constant. The increasing continuously CEC did not appear to be a more sensitive indicator. Although total C content (TC) and total N content (TN) were of limited value in assessing compost maturity, C/N ratio decreased significantly. NH4+-N declined within the first 14 days of composting, while NO3--N did not increase until 14+ days. According to seed germination index (GI), when composting time was prolonged, the toxicity of SMC had a tendency to decrease. Results from the experiments indicate that 21 days after rapid composting, SMC is considered to be mature. It is therefore concluded that pH, EC and GI have potential as useful indicators for SMC maturity evaluation.
堆肥的成熟度是农业和园艺生产中一个重要的实用因素。本研究对蘑菇废堆肥(SMC)进行了成熟度评价。采用标准化学分析(pH、EC、CEC、总C和总N含量、C/N比、NH4+-N、NO3——N)评价堆肥成熟度。随后,利用芥蓝种子萌发生物测定法评估了SMC的潜在植物毒性。在28 d快速堆肥期间,从单窗定期收集SMC样品。随着堆肥时间的延长,pH和EC值都有增加的趋势,最终两者保持不变。持续增加的CEC似乎不是一个更敏感的指标。尽管全C含量(TC)和全N含量(TN)在评价堆肥成熟度方面价值有限,但C/N显著降低。NH4+-N在堆肥前14 d内呈下降趋势,NO3——N直到14 d后才有所增加。根据种子萌发指数(GI),随着堆肥时间的延长,SMC的毒性有降低的趋势。试验结果表明,快速堆肥21天后,SMC成熟。因此,pH、EC和GI有潜力作为SMC成熟度评价的有用指标。
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引用次数: 0
Review of High Arsenic Groundwater in China 中国高砷地下水研究进展
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5517266
Zhiping Li, D. van Halem, J. Verberk
High arsenic groundwater and associated health risks caused by drinking high-arsenic groundwater are a great headache in many parts of the world, including China. Occurrence and distribution of high arsenic groundwater in China were firstly stated. High arsenic groundwater mainly exists in Inner Mongolia, Shanxi, Xinjiang et al, 40 counties of 8 provinces. Furthermore, arsenic mobilization processes in China were discussed by means of analyzing two typical high arsenic groundwater areas: Hetao Plain, in Inner Mongolia and Datong Basin in Shanxi Province. Even if some relevant experiments research have been carried out, changing another safe water source may be more feasible in China if there is some one available.
在包括中国在内的世界许多地区,高砷地下水以及饮用高砷地下水引起的相关健康风险是一个令人头疼的问题。首次阐述了中国高砷地下水的赋存状态和分布规律。高砷地下水主要分布在内蒙古、山西、新疆等8个省40个县。此外,通过对内蒙古河套平原和山西大同盆地两个典型高砷地下水区进行分析,探讨了砷在中国的运移过程。即使已经进行了一些相关的实验研究,如果中国有其他的安全水源,换一个安全水源可能更可行。
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引用次数: 2
Study of Mathematical Model in Electrodialysis of Desalination 海水淡化电渗析的数学模型研究
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516941
G. Jing, L. Xing, Shulin Li
This paper presents experimental data and mathematical model for a laboratory scale electrodialysis (ED) cell. Mathematical model for desalination by electrodialysis (ED) was established on the basis of NaCl separation experimental data. The mathematical model started from a differential equation of steady-state mass balance, derived the equation and was validated by experiments. The model parameter was determined by the experiment data. The model gives concentration of dilute compartment for various voltages, flow rates and feed concentrations. Comparing predicted data with experimental data, excellent agreement between the experimental data and the model results indicates the capability of mathematical model to predict the desalination process.
本文介绍了实验室规模的电渗析(ED)电池的实验数据和数学模型。以NaCl分离实验数据为基础,建立了电渗析海水淡化的数学模型。该数学模型从稳态质量平衡微分方程出发,推导出方程,并通过实验进行了验证。模型参数由实验数据确定。该模型给出了不同电压、流速和进料浓度下稀释室的浓度。将预测数据与实验数据进行比较,实验数据与模型结果吻合良好,表明数学模型具有预测海水淡化过程的能力。
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引用次数: 2
Landscape Monitoring and Dynamic Evolution of Wetland Resources in Beijing 北京湿地资源景观监测与动态演变
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5515490
L. Gu, Xin-jie Wang, Zhi-wen Gong, Jin Wang, Hao Xu
It is very significant for urban development and urban wetland protection. Wetland, considered as the natural ecosystem and landscape style, has great resource potentiality and environmental function. For a long time, the change of wetland landscape pattern has been affected by human activity, and has changed the existing wetland function, and exerted influence on wetland environment. In this paper, the capital city of Beijing is for the study of wetland areas, and we used Markov Model to analysis the dynamic change and the trend evolution. Supported by RS and GIS technology, combined the achievements in previous researches and numerous field investigations, we used Landsat-TM images of 1978, 1974, 1991, 1996, 2001 and 2005 as the information source. Several landscape indices were used to evaluate the changes of landscape pattern. The results showed that Beijing wetlands recourses degraded significantly in the past three decade, the area had dropped by 54.0%, from 68491.63 hm2 in 1978 to 31416.31 hm2 in 2005; The patches of wetlands tended to be irregular, and the degree of fragmentation was increasing, thus the wetlands would be easy to cause regression, and its function was difficult to exert; The transition probability matrices at two primary states (1996 and 2005) were established. Then, the development and the future tendency of wetland landscape patterns are simulated and forecasted with Markov process. Most of the natural wetlands areas would be increasing and urban area decreasing, which showed urbanization process has great impact on wetland landscape pattern.
对城市发展和城市湿地保护具有重要意义。湿地作为一种自然生态系统和景观类型,具有巨大的资源潜力和环境功能。长期以来,湿地景观格局的变化受到人类活动的影响,改变了现有湿地的功能,对湿地环境产生了影响。本文以首都北京市为研究对象,采用马尔可夫模型分析其动态变化和趋势演变。在RS和GIS技术的支持下,结合以往的研究成果和大量的实地调查,我们以1978年、1974年、1991年、1996年、2001年和2005年的Landsat-TM影像为信息源。利用几种景观指数对景观格局的变化进行了评价。结果表明:近30年来,北京湿地资源明显退化,面积从1978年的68491.63 hm2减少到2005年的31416.31 hm2,减少了54.0%;湿地斑块呈不规则化趋势,破碎化程度不断增加,容易导致湿地退化,功能难以发挥;建立了1996年和2005年两个主要状态的转移概率矩阵。然后利用马尔可夫过程对湿地景观格局的发展和未来趋势进行了模拟和预测。大部分天然湿地面积呈增加趋势,城市面积呈减少趋势,表明城市化进程对湿地景观格局的影响较大。
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引用次数: 2
Monitoring and Evaluating Aquatic Environment in Mining Subsidence Supported by 3D GIS 基于三维GIS的矿区沉陷水环境监测与评价
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5517822
Yanbin Wu, Ming Huang
The ponding subsidence zone induced by mining cannot be ignored, at the same time, along with the expanding of mining area, water area also increased year by year. To promote resources management effectively and achieve the goal of resources use, monitoring and evaluating aquatic environment in mining subsidence supported by 3D GIS was to be carried out. This information system was established based on 3D GIS, and spatial database to manage multi-source heterogeneous data was proposed in this article, whose core was to spatial modeler, visualization and 3D spatial analysis. The structure and function and key technologies of the 3D GIS system were also studied in this paper, which emphasized on integration of aquatic environment data, water environment monitoring and evaluation model, 3D spatial analysis. The results show that this system can integrate the multi-source heterogeneous spatial data and related properties data into the whole body, demonstrate graphical visualization and analyze compositely. So using this system for resource management in mine area has strong practicability.
开采引起的沉陷带不容忽视,同时,随着矿区面积的扩大,水域面积也逐年增加。为有效促进资源管理,实现资源利用目标,开展三维GIS支持下的矿区沉陷水环境监测与评价。基于三维GIS建立了该信息系统,提出了以空间建模、可视化和三维空间分析为核心的多源异构数据管理空间数据库。研究了三维GIS系统的结构、功能和关键技术,重点研究了水环境数据的集成、水环境监测与评价模型、三维空间分析等。结果表明,该系统能够将多源异构空间数据及相关属性数据整合为一体,进行图形化可视化和组合分析。因此将该系统应用于矿区资源管理具有很强的实用性。
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引用次数: 2
Application of Modified Streeter-Phelps Model and COD Changing Model to Xiangxi River in Three Gorges Reservoir Area 修正Streeter-Phelps模型及COD变化模型在三峡库区香溪河的应用
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5517979
Hui Peng, Wei Yao, Ping Huang
As an often used physical model with good theoretical and practical values, Streeter--Phelps model has been widely used to research water quality at present. However, in some special water areas the utility of Streeter--Phelps Model suffers limitations because confirming reaeration coefficient often comes from tests in rivers with fast water flow speed rather than considering the wavy effects. Furthermore, in practical research in Xiangxi River in Three Gorges Reservoir Area the changing rule of COD appears abnormity. In some water areas far from pollution source the value of COD changing with time doesn't accord with exponential attenuation but slightly sways in a certain value. This phenomenon can not be explained by former modified Streeter--Phelps models. After research it indicates that through modifying the reaeration coefficient good calculation results will be obtained. Similarly, the changing rule of COD not only can be made clear but also can provide scientific evidence to further studying rules of pollution ejection and sediment motion.
Streeter—Phelps模型作为一种常用的物理模型,具有较好的理论和实用价值,目前在水质研究中得到了广泛的应用。然而,在一些特殊水域,Streeter—Phelps模型的实用性受到限制,因为确认再生系数通常来自于水流速度快的河流试验,而不是考虑波浪效应。此外,在三峡库区湘溪河的实际研究中,COD的变化规律也出现了异常。在远离污染源的部分水域,COD随时间的变化值不符合指数衰减,而是在某一数值上略有波动。这一现象不能用先前修正的Streeter- Phelps模型来解释。研究表明,通过对再生系数的修正,可以获得较好的计算结果。同样,COD的变化规律不仅可以明确,而且可以为进一步研究污染喷射和泥沙运动规律提供科学依据。
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引用次数: 3
Steeper Action Potential Duration Restitution Slope Increases Risk of Ventricular Fibrillation: A Simulation Study 更陡的动作电位持续时间恢复斜率增加心室颤动的风险:一项模拟研究
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5515680
Yi Zheng, D. Wei, Zuxiang Fang
Action potential duration restitution (APDR) is thought to be relevant to the stability of cardiac electrophysiology. Ventricular fibrillation (VF) was simulated using the Wei-Harumi model by taking into account piecewise linear action potential duration restitution based on clinical findings. We found, in the simulation, that heart model with steeper slope of APDR required less ectopic stimuli to trigger VF. This result suggested that the APDR may affect cardiac vulnerability to VF.
动作电位持续时间恢复(APDR)被认为与心脏电生理的稳定性有关。采用基于临床结果考虑分段线性动作电位持续时间恢复的Wei-Harumi模型模拟心室颤动(VF)。我们在模拟中发现,APDR斜率更陡的心脏模型需要更少的异位刺激来触发VF。提示APDR可能影响心脏对VF的易感性。
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引用次数: 1
期刊
2010 4th International Conference on Bioinformatics and Biomedical Engineering
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