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2010 4th International Conference on Bioinformatics and Biomedical Engineering最新文献

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T Cell Apoptosis Was Inhibited by Fas of Antisense Oligonucleotide 反义寡核苷酸Fas抑制T细胞凋亡
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516974
Shi-ying Zheng, D. Jiang, J. Ge, Zhengjie Shen
We investigate the effect of specific antisense oligodeoxynucleotide (ASO DN) inhibition of Fas expression on T cell apoptosis induced by gastric carcinoma cell . Fas receptor (Fas) and Fas ligand (FasL) expressed by the gastric carcinoma cell line SGC-7901 and Jurkat T cells were detected by flow cytometry (FCM) and the ability of FasL-inducing T cell apoptosis was tested by co-culture assay in vitro with SGC-7901 cells and Jurkat T cells. The Jurkat cells were transfected with Fas-ASODN using lipofectin, and the effects of Fas-ASODN on Fas mRNA level, Fas expression on T cells surface, and apoptosis were investigated by RT-PCR, FCM and co-culture assay, respectively. SGC-7901 cells expressing functional FasL could induce the apoptosis of Jurkat cells as demonstrated by co-culture assays. After the Jurkat cells were transfected with Fas ASODN, the level of Fas mRNA, the expression rate of Fas and the apoptotic rate induced by gastric carcinoma cells were all decreased. Gastric carcinoma cells expressing FasL can induce apoptosis in Fas-expressing T cells, indicating that transfection of Fas ASODN can partially convert the immune inhibitory condition induced by gastric carcinoma cells. Fas ASODN may be a useful tool in the armamentarium of tumor immune therapy.
研究了特异性反义寡脱氧核苷酸(ASO DN)抑制Fas表达对胃癌细胞诱导的T细胞凋亡的影响。采用流式细胞术检测胃癌细胞系SGC-7901和Jurkat T细胞表达的Fas受体(Fas)和Fas配体(FasL),并与SGC-7901细胞和Jurkat T细胞体外共培养实验检测FasL诱导T细胞凋亡的能力。用脂质体素转染Jurkat细胞Fas- asodn,分别采用RT-PCR、流式细胞术和共培养法观察Fas- asodn对Jurkat细胞Fas mRNA水平、T细胞表面Fas表达及凋亡的影响。共培养实验表明,表达功能性FasL的SGC-7901细胞可诱导Jurkat细胞凋亡。Fas ASODN转染Jurkat细胞后,Fas mRNA水平、Fas表达率及胃癌细胞凋亡率均降低。表达FasL的胃癌细胞可诱导表达FasL的T细胞凋亡,说明转染Fas ASODN可部分转化胃癌细胞诱导的免疫抑制状态。Fas ASODN可能成为肿瘤免疫治疗的重要工具。
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引用次数: 0
Microwave-Assisted COD Removal from Landfill Leachate by Hydrogen Peroxide, Peroxymonosulfate and Persulfate 双氧水、过氧单硫酸盐和过硫酸盐微波辅助去除垃圾渗滤液中的COD
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5517536
Wenyi Zhang, Shiying Yang, Rui Niu, Xueting Shao, Liang Shan, Xin Yang, Ping Wang
H2O2-based advanced oxidation processes (AOPs) and SO4•−-based AOPs were proved novel and promising techniques in treatment of wasterwater. Microwave (MW)-assisted hydrogen peroxide (H2O2), peroxymonosulfate (PMS) and persulfate (PS) were used to dispose of COD in landfill leachate. Oxidant dosage and chloride concentration were investigated as the major operating conditions. The initial COD is 4062.8 mg/L. It is demonstrated that MW-assisted H2O2, PMS and PS can effectively degrade leachate organics, and the COD removal can reach 43.5%, 80.2%, 97.3% when the oxidant concentration is 0.3 mol/L, respectively. Chloride ion will slightly improve the reaction rate for H2O2 and PS, while a little slow down for PMS, but all the eventual COD removal efficiencies keep stable. Generally speaking, MW-assisted peroxide is a powerful means in degradation of COD in landfill leachate.
基于h2o2的深度氧化工艺(AOPs)和基于SO4•−的深度氧化工艺在污水处理中被证明是新颖而有前途的技术。采用微波(MW)辅助过氧化氢(H2O2)、过氧单硫酸盐(PMS)和过硫酸盐(PS)处理垃圾渗滤液中的COD。考察了氧化剂用量和氯离子浓度为主要操作条件。初始COD为4062.8 mg/L。结果表明,mw辅助H2O2、PMS和PS能有效降解渗滤液中的有机物,当氧化剂浓度为0.3 mol/L时,COD去除率分别可达43.5%、80.2%和97.3%。氯离子对H2O2和PS的反应速率略有提高,对PMS的反应速率略有减慢,但最终的COD去除率保持稳定。一般来说,mw辅助过氧化物是降解垃圾渗滤液中COD的有效手段。
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引用次数: 12
Phosphates Recovery through Hydroxyapatite Crystallization from Wastewater Using Converter Slag as a Seed Crystal 以转炉炉渣为种晶羟基磷灰石结晶法回收废水中的磷酸盐
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5518291
J. Duan, Yin-lan Cao, Bi-yan He
With powdered converter slag used as a seed crystal, the effects of slag dosage, pH, temperature, and Ca2+ concentration on phosphate removal from synthetic solution through crystallization were investigated in batch experiments. The results indicated that slag concentration of 1.7 g/L, pH value of 9-10, reaction time of 3h , reaction temperature of 20-25℃, Ca/P molar ratio of 2, seemed to be the favorable conditions. Under the optimal reaction condition, the removal efficiencies of the swine farm wastewater and the supernate of concentrated sludge , were 95.36% and 96.54%, respectively. After converter slag as a seed material was used for ten times, the P removal efficiency was still above 80%. The Scanning electron micrograph (SEM) images and X-ray diffraction (XRD) pattern of converter slag before and after phosphorus crystallization confirmed the formation of crystalline hydroxyapatite. Energy dispersive X-ray spectroscopy (EDS) mapping analysis revealed that the molar composition ratio of Ca/P of the crystalline material was 1.72. The Ca/P molar ratio > 1.67 for crystalline substance might result from the presence of CaCO3 on the crystalline surfaces. The results indicated that total P contents in the generated crystallization products of up to 14.5 % P-tot (33.2 % P2O5) were achieved, and was comparable to natural phosphate rock.
以粉末状转炉炉渣为种晶,通过批量实验研究了炉渣用量、pH、温度和Ca2+浓度对合成溶液结晶除磷的影响。结果表明,渣浓度为1.7 g/L、pH值为9 ~ 10、反应时间为3h、反应温度为20 ~ 25℃、Ca/P摩尔比为2为最佳条件。在最佳反应条件下,猪场废水和浓缩污泥上清液的去除率分别为95.36%和96.54%。转炉炉渣作为种料使用10次后,除磷效率仍在80%以上。磷结晶前后转炉渣的扫描电镜(SEM)和x射线衍射(XRD)图证实了结晶羟基磷灰石的形成。能量色散x射线能谱(EDS)作图分析表明,晶体材料的Ca/P摩尔组成比为1.72。结晶物质的Ca/P摩尔比大于1.67可能是由于CaCO3存在于结晶表面所致。结果表明,结晶产物中总磷含量可达14.5% P-tot (33.2% P2O5),与天然磷矿相当。
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引用次数: 3
Status and Prospects of Music Electrotherapy 音乐电疗的现状与展望
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5514922
H. Zhang, H. Hua
Abstract-Music electrotherapy is on the basis of electro-acupuncture, which combined music,electrode with electro-acupuncture. This method draws on the characteristics of both electrotherapy and music therapy music. Music electrotherapy through music and music signals acting on the hearing and body, increase or improve the person's psychological, physiological conditions, so as to achieve health, disease prevention and rehabilitation purposes. In this article, some new ideas and methods based on programmable and distributed automatic system are proposed, in order to improve and perfect the existing music electrotherapy instruments.
摘要:音乐电疗是在电针的基础上,将音乐电极与电针相结合。这种方法借鉴了电疗和音乐疗法的特点。音乐电疗是通过音乐和音乐信号作用于听觉和身体,增加或改善人的心理、生理状况,从而达到保健、预防疾病和康复的目的。为了改进和完善现有的音乐电疗仪器,本文提出了基于可编程和分布式自动化系统的一些新思路和新方法。
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引用次数: 1
Inhibition of Gene Expression of Trehalase Enhances Drought Resistance in Transgenic Tobacco 抑制海藻糖酶基因表达增强转基因烟草抗旱性
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516954
Bei Guo, Y. Song, Xuefeng Guo, Wenping Wang, Ning Ding
The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 - phosphate synthase, TPS), P3 (with gene of trehalose -6 - phosphate phosphatase, TPP), a3 (with anti-TRE gene), i2 (with RNAi-TRE gene), were selected for identification of drought resistance, and the Malondialdehyde (MDA), relative conductivity have been measured after water-stress during this experiment. The results showed that the transgenic tobaccos with anti-TRE and RNAi-TRE grew better than other individuals under water-stress conditions; conductivity of i2 was lower than other plants. Meantime, i2 has performance of some special features. These indicate that through application the antisense RNA and RNA interference technology to trehalase gene, it is possible to make the receptor trehalose metabolism changes. The inhibition of gene expression of trehalase can led to the accumulation of trehalose content and the enhancement of drought resistance in the transgenic tobaccos.
选择代表性个体CK(非转基因)、121(携带pBI121载体)、1285(携带pGSA1285载体)、T4(携带海藻糖酶基因TRE)、S1(携带海藻糖-6 -磷酸合酶基因TPS)、P3(携带海藻糖-6 -磷酸合酶基因TPP)、a3(携带抗TRE基因)、i2(携带RNAi-TRE基因)进行抗旱性鉴定,并在本试验中测定了胁迫后丙二醛(MDA)的相对电导率。结果表明,抗- tre和RNAi-TRE转基因烟草在水分胁迫条件下的生长情况优于其他个体;i2的电导率低于其他植株。同时,i2具有一些特殊的性能。这说明通过对海藻糖酶基因应用反义RNA和RNA干扰技术,有可能使受体海藻糖代谢发生改变。抑制海藻糖酶基因表达可使转基因烟草海藻糖含量积累,抗旱性增强。
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引用次数: 3
Sand-Dust Aerosol's Impact on Urban Environment in Xinjiang 新疆沙尘气溶胶对城市环境的影响
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5515151
W. Gao, Yu-jiang Yuan, Zhihui Liu, Wei Wei
Xinjiang is the emission region of sand-dust aerosol as well as the region suffering the severest damage from sand-dust aerosol, whose urban environment especially suffers greater impact from the particles of sand-dust aerosol. The impact on urban environment in Xinjiang is mainly attributed to the sand-dust weather in the multiple sand-dust seasons. The variation trend is in conformity with that of the sand-dust weather. Upon the occurrence of the sand-dust weather, PM10 of the city increases sharply. The sand-dust aerosol turns into the most important pollutant. In the multiple sand-dust seasons, the sand-dust aerosol is the primary pollutant. In the non-heating season, the content percentage of the sand-dust aerosol in the pollutants of Crumbs reaches up to 37% and PM10 during the sand-dust weather period changes obviously, indicating the sand-dust weather has great impact on the atmosphere quality of Xinjiang.
新疆是沙尘气溶胶的排放区,也是沙尘气溶胶危害最严重的地区,尤其是沙尘气溶胶颗粒对城市环境的影响更大。对新疆城市环境的影响主要是由于多个沙尘季节的沙尘天气。其变化趋势与沙尘天气的变化趋势一致。沙尘天气发生后,城市PM10急剧增加。沙尘气溶胶成为最重要的污染物。在多个沙尘季节中,沙尘气溶胶是主要污染物。非采暖季,碎屑污染物中沙尘气溶胶含量百分比高达37%,沙尘天气期PM10变化明显,说明沙尘天气对新疆大气质量影响较大。
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引用次数: 0
Biological Treatment of Ammonium-Rich Wastewater with the Partial Nitrification and Anaerobic Ammonium Oxidation 部分硝化-厌氧氨氧化生物处理富氨废水
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5517526
Q. Xie, Xiaoxia Li, S. Bai, Yan-hong Li
The aim of the investigation was to evaluate the nitrogen removal efficiency by using the combined partial nitrification and anaerobic ammonium oxidation (anammox) process for high-strength ammonium synthetic wastewater. The partial nitrification and anaerobic ammonium oxidation reactors were inoculate with domesticated sludge and operated respectively during the startup period. Subsequently, they were combined for stable operation when the partial nitrification effluent was suitable for being the influent of the anaerobic ammonium oxidation. The startup period was about 40 days. The experimental results of the nitrogen removal performance during the startup and the stable operation periods indicate that over 90% of the inlet nitrogen load to the combined reactors was removed. With the influent concentrations of 360 - 400mg NH4+-N /L, the mean NH4+-N, NO2--N and TN concentrations of the combined process effluent were 2.7mg/L, 0.51mg/L and 55.9mg/L, respectively. And their mean removal efficiencies were 93.7%, 96.2% and 84.6%, correspondingly.
研究了部分硝化-厌氧氨氧化联合工艺处理高强度氨合成废水的脱氮效果。部分硝化反应器和厌氧氨氧化反应器在启动阶段分别接种驯化污泥进行运行。当部分硝化出水适合作为厌氧氨氧化进水时,将两者组合稳定运行。启动时间约为40天。启动和稳定运行期间的脱氮性能实验结果表明,组合反应器进口氮负荷的脱氮率达到90%以上。进水NH4+-N浓度为360 ~ 400mg时,联合工艺出水NH4+-N、NO2——N和TN平均浓度分别为2.7mg/L、0.51mg/L和55.9mg/L。平均去除率分别为93.7%、96.2%和84.6%。
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引用次数: 2
Optimal Conditions for Biodegradation of Indeno (1,2,3-cd) Pyrene in Soil Slurry Reactors 土壤污泥反应器生物降解吲哚(1,2,3-cd)芘的最佳条件
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5517395
Haiying Chen, A. Ding, Junfeng Dou, Lirong Cheng, Fuqiang Fan, Yongchao Du, Xiaona Liu
Soil slurry reactor technology is one of the effective methods for treating PAHs contaminated soil. In this paper, removal of high molecular weight PAHs, Indeno (1,2,3-cd) pyrene (Inp), was studied in soil slurry reactors with isolated bacteria from PAHs contaminated soil. The factors affecting Inp biodegradation and the optimal conditions were investigated using orthogonal experimental design (L9(34)). The highest degradation efficiency of Inp was 50.71% after the incubation of 12 days. The most significant influencing factors were aeration rate, water to soil ratio and salinity, while pH was less significant. The optimal conditions for Inp degradation in soil slurry reactor was: aeration rate 120L/h, water to soil ratio 2:1, salinity 10%, pH=6.
土浆反应器技术是处理多环芳烃污染土壤的有效方法之一。本文以多环芳烃污染土壤中分离的细菌为研究对象,在土壤浆体反应器中对高分子量多环芳烃(Inp)进行了去除研究。采用正交试验设计(L9(34))对影响Inp生物降解的因素和最佳条件进行了研究。培养12 d后,Inp的最高降解率为50.71%。最显著的影响因子是通气量、水土比和盐度,pH不太显著。土浆反应器降解Inp的最佳条件为:曝气速率120L/h,水土比2:1,矿化度10%,pH=6。
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引用次数: 0
Fluoride Adsorption onto a Ce-La Binary Hydroxide Adsorbent as Recovery of Ceria Glass Polishing Wastes 铈-镧二元氢氧化物吸附剂对氟化物的吸附回收氧化铈玻璃抛光废料
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516166
Ping Zhang, T. Ouyang, Shanshan Chen, Ruoshang Li, Limin Lai
The adsorption of fluoride onto a Ce−La binary hydroxide adsorbent, herein referred to as CLH adsorbent, was studied under various pH values, adsorbent dose, contact time, and the presence of major anions. In the pH range of 4 to 8, the fluoride adsorption onto CLH reaches a maximum, then decreases with further increasing of pH. A considerably higher adsorption capacity (77.4−89.5 mg g-1) was maintained until the solution pH was up to 8 compared with that of the commercially available activated alumina; indicative of the CLH material should be effective to fluoride removal for major fresh water supplies. The effect of contact time was dependent on the initial fluoride concentration but adsorption equilibriums were reached after 120 min under the initial concentrations of 10 and 25 mg L-1, whereas a residual F- concentration of < 1.5 mg F L-1 was attained that satisfied the guideline value suggested by WHO for fluoride. The adsorptive rate of fluoride on the adsorbent obeys pseudo-second order kinetic models (R2 > 0.99). Adsorption isotherm at pH 7.0 fits best the Langmuir than the Freundlich model of adsorption over the F- concentration range 2−250 mg L-1. A combination of coulombic interaction and the formation of inner-sphere complexes between the activated surface sites and fluoride anions seems to be the adsorption mechanism. Major co-existing anions reduced fluoride adsorption according to their affinity on the CLH surface in the following order: HPO42- > HCO3- ≥ SiO32- > SO42- > Cl-, NO3-.
研究了不同pH值、吸附剂剂量、接触时间和主要阴离子存在情况下,铈−La二元氢氧化物吸附剂(简称CLH吸附剂)对氟的吸附。在pH值为4 ~ 8的范围内,CLH对氟的吸附达到最大值,随后随着pH值的进一步增大而减小,直到溶液pH值达到8时,与市售活性氧化铝相比,CLH对氟的吸附量仍保持在77.4−89.5 mg g-1;表明CLH材料应该对主要淡水供应的除氟有效。接触时间的影响取决于初始氟化物浓度,但在初始浓度为10和25 mg L-1的情况下,在120 min后达到吸附平衡,而剩余F-浓度< 1.5 mg F- 1,满足WHO建议的氟化物指导值。吸附剂对氟的吸附速率服从准二级动力学模型(R2 >0.99)。pH 7.0时的吸附等温线最适合Langmuir模型,而不是Freundlich吸附模型,F-浓度范围为2−250 mg L-1。活化表面位点与氟离子之间的库仑相互作用和球内配合物的形成可能是吸附机理。主要共存阴离子根据其在CLH表面的亲和力降低氟吸附的顺序为:HPO42- > HCO3- ≥sio2 - > SO42- > Cl-, NO3-。
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引用次数: 1
Pesticide Knowledge, Attitudes and Practices in China 中国的农药知识、态度和实践
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516589
Daqi Xu
Pesticides are widely used in China for crop protection. Econometric analysis showed the magnitude of the chronic health effects and health costs to be directly related to pesticide exposure, and accumulative pesticides has led to serious pollution to the environment. This study was conducted on a random sample of 150 rice farming households to collect information on their knowledge, attitude, and behavior related to pesticides in the Anhui province, south of China. Most of farmers we fund did not took incomplete preventive measures. Pesticide information, instruction, and training among farmers should be promoted, and governmental intervention is needed to ensure proper management regarding public health risks and environmental hazards.
农药在中国被广泛用于作物保护。计量经济学分析表明,慢性健康效应的大小和健康成本与农药暴露直接相关,农药的累积已导致严重的环境污染。本研究以中国南方安徽省150户水稻农户为随机样本,收集农户对农药的知识、态度和行为信息。我们资助的大多数农民没有采取完全的预防措施。应促进对农民的农药信息、指导和培训,并需要政府干预,以确保对公共健康风险和环境危害进行适当管理。
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引用次数: 2
期刊
2010 4th International Conference on Bioinformatics and Biomedical Engineering
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