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Effects of altering the ratio of C16:0 and cis-9 C18:1 in rumen bypass fat on growth performance, lipid metabolism, intestinal barrier, cecal microbiota, and inflammation in fattening bulls. 改变瘤胃旁路脂肪中 C16:0 和 cis-9 C18:1 的比例对育肥公牛生长性能、脂质代谢、肠道屏障、盲肠微生物群和炎症的影响。
IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-07 DOI: 10.1186/s40104-024-01052-1
Haixin Bai, Haosheng Zhang, Congwen Wang, Modinat Tolani Lambo, Yang Li, Yonggen Zhang

Background: C16:0 and cis-9 C18:1 may have different effects on animal growth and health due to unique metabolism in vivo. This study was investigated to explore the different effects of altering the ratio of C16:0 and cis-9 C18:1 in fat supplements on growth performance, lipid metabolism, intestinal barrier, cecal microbiota, and inflammation in fattening bulls. Thirty finishing Angus bulls (626 ± 69 kg, 21 ± 0.5 months) were divided into 3 treatments according to the randomized block design: (1) control diet without additional fat (CON), (2) CON + 2.5% palmitic acid calcium salt (PA, 90% C16:0), and (3) CON + 2.5% mixed fatty acid calcium salt (MA, 60% C16:0 + 30% cis-9 C18:1). The experiment lasted for 104 d, after which all the bulls were slaughtered and sampled for analysis.

Results: MA tended to reduce 0-52 d dry matter intake compared to PA (DMI, P = 0.052). Compared with CON and MA, PA significantly increased 0-52 d average daily gain (ADG, P = 0.027). PA tended to improve the 0-52 d feed conversion rate compared with CON (FCR, P = 0.088). Both PA and MA had no significant effect on 52-104 days of DMI, ADG and FCR (P > 0.05). PA tended to improve plasma triglycerides compared with MA (P = 0.077), significantly increased plasma cholesterol (P = 0.002) and tended to improve subcutaneous adipose weight (P = 0.066) when compared with CON and MA. Both PA and MA increased visceral adipose weight compared with CON (P = 0.021). Only PA increased the colonization of Rikenellaceae, Ruminococcus and Proteobacteria in the cecum, and MA increased Akkermansia abundance (P < 0.05). Compared with CON, both PA and MA down-regulated the mRNA expression of Claudin-1 in the jejunum (P < 0.001), increased plasma diamine oxidase (DAO, P < 0.001) and lipopolysaccharide (LPS, P = 0.045). Compared with CON and MA, PA down-regulated the ZO-1 in the jejunum (P < 0.001) and increased plasma LPS-binding protein (LBP, P < 0.001). Compared with CON, only PA down-regulated the Occludin in the jejunum (P = 0.013). Compared with CON, PA and MA significantly up-regulated the expression of TLR-4 and NF-κB in the visceral adipose (P < 0.001) and increased plasma IL-6 (P < 0.001). Compared with CON, only PA up-regulated the TNF-α in the visceral adipose (P = 0.01). Compared with CON and MA, PA up-regulated IL-6 in the visceral adipose (P < 0.001), increased plasma TNF-α (P < 0.001), and reduced the IgG content in plasma (P = 0.035). Compared with CON, PA and MA increased C16:0 in subcutaneous fat and longissimus dorsi muscle (P < 0.05), while more C16:0 was also deposited by extension and desaturation into C18:0 and cis-9 C18:1. However, neither PA nor MA affected the content of cis-9 C18:1 in longissimus dorsi muscle compared with CON (P > 0.05).

Conclusions: MA containing 30% cis-9 C18:1 reduced the risk of high C16:0 dietary fat induced subcutaneous fat obesity, adipose tissue and systemic low-grade inflamma

背景:由于 C16:0 和顺式-9 C18:1 在体内的代谢方式不同,它们可能对动物的生长和健康产生不同的影响。本研究旨在探讨改变脂肪补充剂中 C16:0 和顺式-9 C18:1 的比例对育肥公牛生长性能、脂质代谢、肠道屏障、盲肠微生物群和炎症的不同影响。按照随机区组设计,将 30 头育成安格斯公牛(626 ± 69 kg,21 ± 0.5 月龄)分为 3 个处理:(1)不添加脂肪的对照日粮(CON);(2)CON + 2.5% 棕榈酸钙盐(PA,90% C16:0);(3)CON + 2.5% 混合脂肪酸钙盐(MA,60% C16:0 + 30% cis-9 C18:1)。实验持续了 104 天,之后所有公牛被屠宰并取样分析:与 PA 相比,MA 有降低 0-52 d 干物质摄入量的趋势(DMI,P = 0.052)。与 CON 和 MA 相比,PA 能显著提高 0-52 d 平均日增重(ADG,P = 0.027)。与 CON 相比,PA 有提高 0-52 d 饲料转化率的趋势(FCR,P = 0.088)。PA 和 MA 对 52-104 天的 DMI、ADG 和 FCR 均无明显影响(P > 0.05)。与 MA 相比,PA 有改善血浆甘油三酯的趋势(P = 0.077);与 CON 和 MA 相比,PA 有显著增加血浆胆固醇的趋势(P = 0.002);与 CON 和 MA 相比,PA 有改善皮下脂肪重量的趋势(P = 0.066)。与 CON 相比,PA 和 MA 均增加了内脏脂肪重量(P = 0.021)。只有 PA 增加了 Rikenellaceae、Ruminococcus 和 Proteobacteria 在盲肠中的定植,MA 增加了 Akkermansia 的丰度(P 0.05):含 30% cis-9 C18:1 的 MA 通过加速脂肪酸氧化利用、改善 Akkermansia 的定植、减少肠屏障损伤和下调 NF-κB 激活,降低了高 C16:0 膳食脂肪诱发的皮下脂肪肥胖、脂肪组织和全身低度炎症的风险。
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引用次数: 0
Source and level of dietary iron influence semen quality by affecting inflammation, oxidative stress and iron utilization levels in boars. 膳食铁的来源和水平会影响公猪的炎症、氧化应激和铁利用水平,从而影响精液质量。
IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-06 DOI: 10.1186/s40104-024-01032-5
Yinghui Wu, Yamei Li, Yueyue Miao, Hongkui Wei, Hefeng Luo, Chunxiao Ren, Yawei Zhang, Juan Chen, Tanghong Wei, Jiyan Deng, Jian Peng

Background: Boars fed a mixed form of inorganic and organic iron in excess of the NRC recommended levels still develop anemia, which suggested that the current level and form of iron supplementation in boar diets may be inappropriate. Therefore, 56 healthy Topeka E line boars aged 15-21 months were randomly divided into 5 groups: basal diet supplemented with 96 mg/kg ferrous sulfate (FeSO4) and 54 mg/kg glycine chelated iron (Gly-Fe, control); 80 mg/kg or 115 mg/kg Gly-Fe; 80 mg/kg or 115 mg/kg methionine hydroxyl analogue chelated iron (MHA-Fe, from Calimet-Fe) for 16 weeks. The effects of dietary iron supplementation with different sources and levels on semen quality in boars were investigated.

Results: 1) Serum Fe and hemoglobin concentrations were not affected by reduced dietary iron levels in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups compared with the control group (P > 0.05). 2) Serum interleukin-6 (IL-6) and sperm malondialdehyde (MDA) levels in the 80 mg/kg or 115 mg/kg MHA-Fe groups were lower than those in the control group (P < 0.05), and higher serum superoxide dismutase levels and lower MDA levels in the 115 mg/kg MHA-Fe group (P < 0.05). 3) Boars in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups had lower serum hepcidin (P < 0.01), ferritin (P < 0.05), and transferrin receptor (P < 0.01) concentrations, and boars in the 115 mg/kg MHA-Fe group had higher seminal plasma Fe concentrations compared with the control group. 4) Boars in the 80 mg/kg and 115 mg/kg MHA-Fe groups had lower abnormal sperm rate and in situ oscillating sperm ratio compared to the control group at weeks 12 and/or 16 of the trial. However, the effect of Gly-Fe on improving semen quality in boars was not evident. 5) Serum IL-6 level was positively correlated with hepcidin concentration (P < 0.05), which in turn was significantly positively correlated with abnormal sperm rate (P < 0.05). Furthermore, significant correlations were also found between indicators of iron status and oxidative stress and semen quality parameters.

Conclusions: Dietary supplementation with 80 mg/kg or 115 mg/kg MHA-Fe did not induce iron deficiency, but rather reduced serum inflammatory levels and hepcidin concentration, alleviated oxidative stress, increased body iron utilization, and improved semen quality in adult boars.

背景:公猪饲喂的无机铁和有机铁的混合形式超过了 NRC 推荐的水平,但仍会出现贫血,这表明公猪日粮中目前的补铁水平和形式可能不合适。因此,56 头 15-21 月龄的健康托皮卡 E 系公猪被随机分为 5 组:基础日粮添加 96 毫克/千克硫酸亚铁(FeSO4)和 54 毫克/千克甘氨酸螯合铁(Gly-Fe,对照组);80 毫克/千克或 115 毫克/千克 Gly-Fe;80 毫克/千克或 115 毫克/千克蛋氨酸羟基类似物螯合铁(MHA-Fe,来自 Calimet-Fe),持续 16 周。研究了不同来源和含量的铁对公猪精液质量的影响:1)与对照组相比,80 毫克/千克或 115 毫克/千克 Gly-Fe 和 MHA-Fe 组的血清铁和血红蛋白浓度不受日粮铁含量降低的影响(P > 0.05)。膳食补充 80 毫克/千克或 115 毫克/千克 MHA-Fe 不会诱发铁缺乏症,反而会降低血清炎症水平和血红素浓度,减轻氧化应激,提高体内铁的利用率,改善成年公猪的精液质量。
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引用次数: 0
Heat stress affects mammary metabolism by influencing the plasma flow to the glands. 热应激会影响流向乳腺的血浆,从而影响乳腺的新陈代谢。
IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-05 DOI: 10.1186/s40104-024-01050-3
Jia Zeng, Diming Wang, Huizeng Sun, Hongyun Liu, Feng-Qi Zhao, Jianxin Liu

Background: Environmental heat stress (HS) can have detrimental effects on milk production by compromising the mammary function. Mammary plasma flow (MPF) plays a crucial role in nutrient supply and uptake in the mammary gland. In this experiment, we investigated the physiological and metabolic changes in high-yielding cows exposed to different degrees of HS: no HS with thermal-humidity index (THI) below 68 (No-HS), mild HS (Mild-HS, 68 ≤ THI ≤ 79), and moderate HS (Mod-HS, 79 < THI ≤ 88) in their natural environment. Our study focused on the changes in blood oxygen supply and mammary glucose uptake and utilization.

Results: Compared with No-HS, the MPF of dairy cows was greater (P < 0.01) under Mild-HS, but was lower (P < 0.01) in cows under Mod-HS. Oxygen supply and consumption exhibited similar changes to the MPF under different HS, with no difference in ratio of oxygen consumption to supply (P = 0.46). The mammary arterio-vein differences in glucose concentration were lower (P < 0.05) under Mild- and Mod-HS than under no HS. Glucose supply and flow were significantly increased (P < 0.01) under Mild-HS but significantly decreased (P < 0.01) under Mod-HS compared to No-HS. Glucose uptake (P < 0.01) and clearance rates (P < 0.01) were significantly reduced under Mod-HS compared to those under No-HS and Mild-HS. Under Mild-HS, there was a significant decrease (P < 0.01) in the ratio of lactose yield to mammary glucose supply compared to that under No-HS and Mod-HS, with no difference (P = 0.53) in the ratio of lactose yield to uptaken glucose among different HS situations.

Conclusions: Degrees of HS exert different influences on mammary metabolism, mainly by altering MPF in dairy cows. The output from this study may help us to develop strategies to mitigate the impact of different degrees of HS on milk production.

背景:环境热应激(HS)会损害乳腺功能,从而对产奶量产生不利影响。乳腺血浆流(MPF)对乳腺的营养供应和吸收起着至关重要的作用。在本实验中,我们研究了高产奶牛在不同程度的恒温恒湿条件下的生理和代谢变化:无恒温恒湿条件,热湿指数(THI)低于68(No-HS);轻度恒温恒湿条件(Mild-HS,68 ≤ THI ≤ 79);中度恒温恒湿条件(Mod-HS,79):与无HS相比,奶牛的MPF更大(P 结论:HS程度不同,对奶牛的影响也不同:HS程度对奶牛的乳腺代谢有不同的影响,主要是通过改变MPF。这项研究的结果可能有助于我们制定策略,减轻不同程度的HS对奶牛产奶量的影响。
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引用次数: 0
Transcriptomic and epigenomic landscapes of muscle growth during the postnatal period of broilers. 肉鸡出生后肌肉生长的转录组和表观基因组图谱
IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-04 DOI: 10.1186/s40104-024-01049-w
Shuang Gu, Qiang Huang, Yuchen Jie, Congjiao Sun, Chaoliang Wen, Ning Yang

Background: Broilers stand out as one of the fastest-growing livestock globally, making a substantial contribution to animal meat production. However, the molecular and epigenetic mechanisms underlying the rapid growth and development of broiler chickens are still unclear. This study aims to explore muscle development patterns and regulatory networks during the postnatal rapid growth phase of fast-growing broilers. We measured the growth performance of Cornish (CC) and White Plymouth Rock (RR) over a 42-d period. Pectoral muscle samples from both CC and RR were randomly collected at day 21 after hatching (D21) and D42 for RNA-seq and ATAC-seq library construction.

Results: The consistent increase in body weight and pectoral muscle weight across both breeds was observed as they matured, with CC outpacing RR in terms of weight at each stage of development. Differential expression analysis identified 398 and 1,129 genes in the two dimensions of breeds and ages, respectively. A total of 75,149 ATAC-seq peaks were annotated in promoter, exon, intron and intergenic regions, with a higher number of peaks in the promoter and intronic regions. The age-biased genes and breed-biased genes of RNA-seq were combined with the ATAC-seq data for subsequent analysis. The results spotlighted the upregulation of ACTC1 and FDPS at D21, which were primarily associated with muscle structure development by gene cluster enrichment. Additionally, a noteworthy upregulation of MUSTN1, FOS and TGFB3 was spotted in broiler chickens at D42, which were involved in cell differentiation and muscle regeneration after injury, suggesting a regulatory role of muscle growth and repair.

Conclusions: This work provided a regulatory network of postnatal broiler chickens and revealed ACTC1 and MUSTN1 as the key responsible for muscle development and regeneration. Our findings highlight that rapid growth in broiler chickens triggers ongoing muscle damage and subsequent regeneration. These findings provide a foundation for future research to investigate the functional aspects of muscle development.

背景:肉鸡是全球增长最快的家畜之一,对动物肉类生产做出了重大贡献。然而,肉鸡快速生长发育的分子和表观遗传学机制仍不清楚。本研究旨在探索快速生长肉鸡出生后快速生长阶段的肌肉发育模式和调控网络。我们测量了康氏鸡(CC)和普利茅斯岩白鸡(RR)42 天的生长性能。我们在孵化后第21天(D21)和第42天随机采集了CC和RR的胸肌样本,用于构建RNA-seq和ATAC-seq文库:结果:随着发育成熟,两个品种的体重和胸肌重量持续增加,CC在每个发育阶段的体重都超过了RR。差异表达分析在品种和年龄两个维度上分别发现了 398 个和 1,129 个基因。在启动子、外显子、内含子和基因间区共注释了75 149个ATAC-seq峰值,其中启动子和内含子区的峰值数量较多。RNA-seq的年龄偏倚基因和品种偏倚基因与ATAC-seq数据相结合进行了后续分析。结果发现,ACTC1和FDPS在D21时上调,通过基因簇富集,这两个基因主要与肌肉结构发育有关。此外,肉鸡在D42时发现了MUSTN1、FOS和TGFB3的显著上调,它们参与了损伤后的细胞分化和肌肉再生,表明它们对肌肉生长和修复起着调控作用:这项研究提供了肉鸡出生后的调控网络,发现 ACTC1 和 MUSTN1 是肌肉发育和再生的关键。我们的研究结果突出表明,肉鸡的快速生长会引发持续的肌肉损伤和随后的再生。这些发现为今后研究肌肉发育的功能方面奠定了基础。
{"title":"Transcriptomic and epigenomic landscapes of muscle growth during the postnatal period of broilers.","authors":"Shuang Gu, Qiang Huang, Yuchen Jie, Congjiao Sun, Chaoliang Wen, Ning Yang","doi":"10.1186/s40104-024-01049-w","DOIUrl":"10.1186/s40104-024-01049-w","url":null,"abstract":"<p><strong>Background: </strong>Broilers stand out as one of the fastest-growing livestock globally, making a substantial contribution to animal meat production. However, the molecular and epigenetic mechanisms underlying the rapid growth and development of broiler chickens are still unclear. This study aims to explore muscle development patterns and regulatory networks during the postnatal rapid growth phase of fast-growing broilers. We measured the growth performance of Cornish (CC) and White Plymouth Rock (RR) over a 42-d period. Pectoral muscle samples from both CC and RR were randomly collected at day 21 after hatching (D21) and D42 for RNA-seq and ATAC-seq library construction.</p><p><strong>Results: </strong>The consistent increase in body weight and pectoral muscle weight across both breeds was observed as they matured, with CC outpacing RR in terms of weight at each stage of development. Differential expression analysis identified 398 and 1,129 genes in the two dimensions of breeds and ages, respectively. A total of 75,149 ATAC-seq peaks were annotated in promoter, exon, intron and intergenic regions, with a higher number of peaks in the promoter and intronic regions. The age-biased genes and breed-biased genes of RNA-seq were combined with the ATAC-seq data for subsequent analysis. The results spotlighted the upregulation of ACTC1 and FDPS at D21, which were primarily associated with muscle structure development by gene cluster enrichment. Additionally, a noteworthy upregulation of MUSTN1, FOS and TGFB3 was spotted in broiler chickens at D42, which were involved in cell differentiation and muscle regeneration after injury, suggesting a regulatory role of muscle growth and repair.</p><p><strong>Conclusions: </strong>This work provided a regulatory network of postnatal broiler chickens and revealed ACTC1 and MUSTN1 as the key responsible for muscle development and regeneration. Our findings highlight that rapid growth in broiler chickens triggers ongoing muscle damage and subsequent regeneration. These findings provide a foundation for future research to investigate the functional aspects of muscle development.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":null,"pages":null},"PeriodicalIF":6.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Progesterone regulates tissue non-specific alkaline phosphatase (TNSALP) expression and activity in ovine utero-placental tissues. 黄体酮调节绵羊子宫胎盘组织中组织非特异性碱性磷酸酶(TNSALP)的表达和活性。
IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-03 DOI: 10.1186/s40104-024-01048-x
Claire Stenhouse, Katherine M Halloran, Emily C Hoskins, Robyn M Moses, Guoyao Wu, Heewon Seo, Gregory A Johnson, Larry J Suva, Dana Gaddy, Fuller W Bazer

Background: Tissue non-specific alkaline phosphatase (TNSALP; encoded by the ALPL gene) has a critical role in the postnatal regulation of phosphate homeostasis, yet how TNSALP activity and expression are regulated during pregnancy remain largely unknown. This study tested the hypothesis that progesterone (P4) and/or interferon tau (IFNT) regulate TNSALP activity during pregnancy in sheep.

Methods: In Exp. 1, ewes were bred and received daily intramuscular injections of either corn oil vehicle (CO) or 25 mg progesterone in CO (P4) for the first 8 days of pregnancy and were hysterectomized on either Day 9, 12, or 125 of gestation. In Exp. 2, ewes were fitted with intrauterine catheters on Day 7 of the estrous cycle and received daily intramuscular injections of 50 mg P4 in CO and/or 75 mg progesterone receptor antagonist (RU486) in CO from Days 8 to 15, and twice daily intrauterine injections of either control proteins (CX) or IFNT (25 µg/uterine horn/d) from Days 11 to 15 (treatment groups: P4 + CX; P4 + IFNT; RU486 + P4 + CX; and RU486 + P4 + IFNT) and were hysterectomized on Day 16.

Results: In Exp. 1, endometria from ewes administered P4 had greater expression of ALPL mRNA than ewes administered CO on Day 12. TNSALP activity appeared greater in the epithelia, stratum compactum stroma, and endothelium of the blood vessels in the endometrium and myometrium from ewes administered P4 than ewes administered CO on Day 12. On Day 125, TNSALP activity localized to uterine epithelial and endothelial cells, independent of P4 treatment. TNSALP activity in placentomes appeared greater in P4 treated ewes and was detected in endothelial cells and caruncular tissue in P4 treated but not CO treated ewes. In Exp. 2, endometrial homogenates from ewes administered RU486 + P4 + CX had lower TNSALP activity those for P4 + CX and P4 + IFNT ewes. Immunoreactive TNSALP protein appeared greater in the mid- and deep-glandular epithelia in RU486 + P4 + CX treated ewes as compared to the other treatment groups. Enzymatic activity appeared greater on the apical surface of the deep glandular epithelia in endometria from ewes treated with RU486 + P4 + CX compared to the other treatment groups.

Conclusions: These results suggest that P4, but not IFNT, regulates the expression and activity of TNSALP in utero-placental tissues and has the potential to contribute to the regulation of phosphate availability that is critical for conceptus development during pregnancy.

背景:组织非特异性碱性磷酸酶(TNSALP;由ALPL基因编码)在出生后的磷酸盐稳态调节中起着至关重要的作用,但TNSALP的活性和表达在妊娠期间如何调节在很大程度上仍是未知的。本研究测试了孕酮(P4)和/或干扰素tau(IFNT)调节绵羊妊娠期间TNSALP活性的假设:在实验 1 中,母羊配种后在妊娠的前 8 天每天肌肉注射玉米油载体(CO)或 25 毫克黄体酮(P4),并在妊娠的第 9、12 或 125 天切除子宫。在实验 2 中,母羊在发情周期的第 7 天安装宫内导管,从第 8 天到第 15 天每天肌肉注射 50 毫克 P4(CO)和/或 75 毫克孕酮受体拮抗剂(RU486)(CO),从第 11 天到第 15 天每天两次宫内注射对照蛋白(CX)或 IFNT(25 µg/uterine horn/d)(治疗组:P4+CX;P4+IFNT;RU486+P4+CX;RU486+P4+IFNT),并在第 16 天切除子宫:在实验 1 中,在第 12 天时,服用 P4 的母羊子宫内膜的 ALPL mRNA 表达量高于服用 CO 的母羊。在第12天,与服用CO的母羊相比,服用P4的母羊子宫内膜和子宫肌层的上皮、基质层和血管内皮的TNSALP活性更高。第125天,TNSALP活性定位于子宫上皮细胞和内皮细胞,与P4处理无关。P4处理过的母羊胎盘中的TNSALP活性更高,P4处理过的母羊内皮细胞和胴体组织中也检测到了TNSALP活性,而CO处理过的母羊则没有。在实验 2 中,服用 RU486 + P4 + CX 的母羊子宫内膜匀浆的 TNSALP 活性低于服用 P4 + CX 和 P4 + IFNT 的母羊。与其他处理组相比,RU486 + P4 + CX 处理组母羊腺上皮中层和深层的免疫活性 TNSALP 蛋白更强。与其他治疗组相比,RU486 + P4 + CX 治疗组母羊子宫内膜深层腺上皮顶端表面的酶活性更高:这些结果表明,P4(而非 IFNT)可调节 TNSALP 在子宫胎盘组织中的表达和活性,并有可能有助于调节磷酸盐的可用性,而磷酸盐的可用性对妊娠期间胎儿的发育至关重要。
{"title":"Progesterone regulates tissue non-specific alkaline phosphatase (TNSALP) expression and activity in ovine utero-placental tissues.","authors":"Claire Stenhouse, Katherine M Halloran, Emily C Hoskins, Robyn M Moses, Guoyao Wu, Heewon Seo, Gregory A Johnson, Larry J Suva, Dana Gaddy, Fuller W Bazer","doi":"10.1186/s40104-024-01048-x","DOIUrl":"10.1186/s40104-024-01048-x","url":null,"abstract":"<p><strong>Background: </strong>Tissue non-specific alkaline phosphatase (TNSALP; encoded by the ALPL gene) has a critical role in the postnatal regulation of phosphate homeostasis, yet how TNSALP activity and expression are regulated during pregnancy remain largely unknown. This study tested the hypothesis that progesterone (P4) and/or interferon tau (IFNT) regulate TNSALP activity during pregnancy in sheep.</p><p><strong>Methods: </strong>In Exp. 1, ewes were bred and received daily intramuscular injections of either corn oil vehicle (CO) or 25 mg progesterone in CO (P4) for the first 8 days of pregnancy and were hysterectomized on either Day 9, 12, or 125 of gestation. In Exp. 2, ewes were fitted with intrauterine catheters on Day 7 of the estrous cycle and received daily intramuscular injections of 50 mg P4 in CO and/or 75 mg progesterone receptor antagonist (RU486) in CO from Days 8 to 15, and twice daily intrauterine injections of either control proteins (CX) or IFNT (25 µg/uterine horn/d) from Days 11 to 15 (treatment groups: P4 + CX; P4 + IFNT; RU486 + P4 + CX; and RU486 + P4 + IFNT) and were hysterectomized on Day 16.</p><p><strong>Results: </strong>In Exp. 1, endometria from ewes administered P4 had greater expression of ALPL mRNA than ewes administered CO on Day 12. TNSALP activity appeared greater in the epithelia, stratum compactum stroma, and endothelium of the blood vessels in the endometrium and myometrium from ewes administered P4 than ewes administered CO on Day 12. On Day 125, TNSALP activity localized to uterine epithelial and endothelial cells, independent of P4 treatment. TNSALP activity in placentomes appeared greater in P4 treated ewes and was detected in endothelial cells and caruncular tissue in P4 treated but not CO treated ewes. In Exp. 2, endometrial homogenates from ewes administered RU486 + P4 + CX had lower TNSALP activity those for P4 + CX and P4 + IFNT ewes. Immunoreactive TNSALP protein appeared greater in the mid- and deep-glandular epithelia in RU486 + P4 + CX treated ewes as compared to the other treatment groups. Enzymatic activity appeared greater on the apical surface of the deep glandular epithelia in endometria from ewes treated with RU486 + P4 + CX compared to the other treatment groups.</p><p><strong>Conclusions: </strong>These results suggest that P4, but not IFNT, regulates the expression and activity of TNSALP in utero-placental tissues and has the potential to contribute to the regulation of phosphate availability that is critical for conceptus development during pregnancy.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":null,"pages":null},"PeriodicalIF":6.3,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Probiotic Lactobacillus rhamnosus GG improves insulin sensitivity and offspring survival via modulation of gut microbiota and serum metabolite in a sow model. 益生菌鼠李糖乳杆菌 GG 在母猪模型中通过调节肠道微生物群和血清代谢物提高胰岛素敏感性和后代存活率
IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-02 DOI: 10.1186/s40104-024-01046-z
Tianle Gao, Ran Li, Liang Hu, Quanfang Hu, Hongmei Wen, Rui Zhou, Peiqiang Yuan, Xiaoling Zhang, Lingjie Huang, Yong Zhuo, Shengyu Xu, Yan Lin, Bin Feng, Lianqiang Che, De Wu, Zhengfeng Fang

Background: Sows commonly experience insulin resistance in late gestation and lactation, causing lower feed intake and milk production, which can lead to higher mortality rates in newborn piglets. The probiotic Lactobacillus rhamnosus GG (LGG) is known to improve insulin resistance. However, whether supplementing LGG can improve insulin sensitivity in sows and enhance lactation performance, particularly the early survival of offspring remains unclear. Hence, we explored the effects and mechanisms of supplementing LGG during late gestation and lactation on sow insulin sensitivity, lactation performance, and offspring survival. In total, 20 sows were randomly allocated to an LGG (n = 10) and control group (n = 10).

Results: In sows, LGG supplementation significantly improved insulin sensitivity during late gestation and lactation, increased feed intake, milk production and colostrum lactose levels in early lactation, and enhanced newborn piglet survival. Moreover, LGG treatment significantly reshaped the gut microbiota in sows, notably increasing microbiota diversity and enriching the relative abundance of insulin sensitivity-associated probiotics such as Lactobacillus, Bifidobacterium, and Bacteroides. Serum metabolite and amino acid profiling in late-gestation sows also revealed decreased branched-chain amino acid and kynurenine serum levels following LGG supplementation. Further analyses highlighted a correlation between mitigated insulin resistance in late pregnancy and lactation by LGG and gut microbiota reshaping and changes in serum amino acid metabolism. Furthermore, maternal LGG enhanced immunity in newborn piglets, reduced inflammation, and facilitated the establishment of a gut microbiota.

Conclusions: We provide the first evidence that LGG mitigates insulin resistance in sows and enhances offspring survival by modulating the gut microbiota and amino acid metabolism.

背景:母猪在妊娠后期和哺乳期通常会出现胰岛素抵抗,导致采食量和产奶量降低,从而导致新生仔猪死亡率升高。众所周知,益生菌鼠李糖乳杆菌 GG(LGG)可改善胰岛素抵抗。然而,补充 LGG 是否能改善母猪的胰岛素敏感性并提高泌乳性能,尤其是提高后代的早期存活率,目前仍不清楚。因此,我们探讨了在妊娠后期和泌乳期补充 LGG 对母猪胰岛素敏感性、泌乳性能和后代存活率的影响和机制。共将 20 头母猪随机分配到 LGG 组(n = 10)和对照组(n = 10):结果:补充 LGG 能显著改善母猪妊娠后期和泌乳期的胰岛素敏感性,提高泌乳早期的采食量、产奶量和初乳乳糖水平,并提高新生仔猪的存活率。此外,LGG 治疗还能显著重塑母猪的肠道微生物群,特别是增加微生物群的多样性,丰富与胰岛素敏感性相关的益生菌(如乳酸杆菌、双歧杆菌和乳杆菌)的相对丰度。妊娠后期母猪血清代谢物和氨基酸分析还显示,补充 LGG 后,支链氨基酸和犬尿氨酸的血清水平有所下降。进一步的分析强调了 LGG 减轻妊娠晚期和哺乳期胰岛素抵抗与肠道微生物群重塑和血清氨基酸代谢变化之间的相关性。此外,母源性 LGG 增强了新生仔猪的免疫力,减少了炎症,并促进了肠道微生物群的建立:我们首次提供了证据,证明 LGG 可通过调节肠道微生物群和氨基酸代谢,减轻母猪的胰岛素抵抗并提高后代存活率。
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引用次数: 0
Improving the accuracy of genomic prediction in dairy cattle using the biologically annotated neural networks framework. 利用生物注释神经网络框架提高奶牛基因组预测的准确性。
IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-07-01 DOI: 10.1186/s40104-024-01044-1
Xue Wang, Shaolei Shi, Md Yousuf Ali Khan, Zhe Zhang, Yi Zhang

Background: Biologically annotated neural networks (BANNs) are feedforward Bayesian neural network models that utilize partially connected architectures based on SNP-set annotations. As an interpretable neural network, BANNs model SNP and SNP-set effects in their input and hidden layers, respectively. Furthermore, the weights and connections of the network are regarded as random variables with prior distributions reflecting the manifestation of genetic effects at various genomic scales. However, its application in genomic prediction has yet to be explored.

Results: This study extended the BANNs framework to the area of genomic selection and explored the optimal SNP-set partitioning strategies by using dairy cattle datasets. The SNP-sets were partitioned based on two strategies-gene annotations and 100 kb windows, denoted as BANN_gene and BANN_100kb, respectively. The BANNs model was compared with GBLUP, random forest (RF), BayesB and BayesCπ through five replicates of five-fold cross-validation using genotypic and phenotypic data on milk production traits, type traits, and one health trait of 6,558, 6,210 and 5,962 Chinese Holsteins, respectively. Results showed that the BANNs framework achieves higher genomic prediction accuracy compared to GBLUP, RF and Bayesian methods. Specifically, the BANN_100kb demonstrated superior accuracy and the BANN_gene exhibited generally suboptimal accuracy compared to GBLUP, RF, BayesB and BayesCπ across all traits. The average accuracy improvements of BANN_100kb over GBLUP, RF, BayesB and BayesCπ were 4.86%, 3.95%, 3.84% and 1.92%, and the accuracy of BANN_gene was improved by 3.75%, 2.86%, 2.73% and 0.85% compared to GBLUP, RF, BayesB and BayesCπ, respectively across all seven traits. Meanwhile, both BANN_100kb and BANN_gene yielded lower overall mean square error values than GBLUP, RF and Bayesian methods.

Conclusion: Our findings demonstrated that the BANNs framework performed better than traditional genomic prediction methods in our tested scenarios, and might serve as a promising alternative approach for genomic prediction in dairy cattle.

背景:生物注释神经网络(BANNs)是一种前馈贝叶斯神经网络模型,它利用基于SNP集注释的部分连接架构。作为一种可解释的神经网络,BANNs 在其输入层和隐藏层分别模拟 SNP 和 SNP 集效应。此外,网络的权重和连接被视为随机变量,其先验分布反映了遗传效应在不同基因组尺度上的表现。然而,其在基因组预测中的应用还有待探索:本研究将 BANNs 框架扩展到基因组选择领域,并利用奶牛数据集探索最佳 SNP 集划分策略。SNP集的划分基于两种策略--基因注释和100 kb窗口,分别称为BANN_gene和BANN_100kb。利用 6558 头、6210 头和 5962 头中国荷斯坦牛的产奶性状、类型性状和一个健康性状的基因型和表型数据,通过五次重复的五倍交叉验证,将 BANNs 模型与 GBLUP、随机森林(RF)、BayesB 和 BayesCπ 进行了比较。结果表明,与 GBLUP、RF 和贝叶斯方法相比,BANNs 框架实现了更高的基因组预测精度。具体来说,在所有性状上,与 GBLUP、RF、BayesB 和 BayesCπ 相比,BANN_100kb 表现出更高的准确性,而 BANN_gene 一般表现出次优的准确性。与 GBLUP、RF、BayesB 和 BayesCπ 相比,BANN_100kb 在所有七个性状上的平均准确率分别提高了 4.86%、3.95%、3.84% 和 1.92%,BANN_gene 在所有七个性状上的准确率分别提高了 3.75%、2.86%、2.73% 和 0.85%。同时,与 GBLUP、RF 和贝叶斯方法相比,BANN_100kb 和 BANN_gene 的总体均方误差值更低:我们的研究结果表明,在我们测试的情况下,BANNs 框架比传统的基因组预测方法表现更好,可以作为奶牛基因组预测的一种有前途的替代方法。
{"title":"Improving the accuracy of genomic prediction in dairy cattle using the biologically annotated neural networks framework.","authors":"Xue Wang, Shaolei Shi, Md Yousuf Ali Khan, Zhe Zhang, Yi Zhang","doi":"10.1186/s40104-024-01044-1","DOIUrl":"10.1186/s40104-024-01044-1","url":null,"abstract":"<p><strong>Background: </strong>Biologically annotated neural networks (BANNs) are feedforward Bayesian neural network models that utilize partially connected architectures based on SNP-set annotations. As an interpretable neural network, BANNs model SNP and SNP-set effects in their input and hidden layers, respectively. Furthermore, the weights and connections of the network are regarded as random variables with prior distributions reflecting the manifestation of genetic effects at various genomic scales. However, its application in genomic prediction has yet to be explored.</p><p><strong>Results: </strong>This study extended the BANNs framework to the area of genomic selection and explored the optimal SNP-set partitioning strategies by using dairy cattle datasets. The SNP-sets were partitioned based on two strategies-gene annotations and 100 kb windows, denoted as BANN_gene and BANN_100kb, respectively. The BANNs model was compared with GBLUP, random forest (RF), BayesB and BayesCπ through five replicates of five-fold cross-validation using genotypic and phenotypic data on milk production traits, type traits, and one health trait of 6,558, 6,210 and 5,962 Chinese Holsteins, respectively. Results showed that the BANNs framework achieves higher genomic prediction accuracy compared to GBLUP, RF and Bayesian methods. Specifically, the BANN_100kb demonstrated superior accuracy and the BANN_gene exhibited generally suboptimal accuracy compared to GBLUP, RF, BayesB and BayesCπ across all traits. The average accuracy improvements of BANN_100kb over GBLUP, RF, BayesB and BayesCπ were 4.86%, 3.95%, 3.84% and 1.92%, and the accuracy of BANN_gene was improved by 3.75%, 2.86%, 2.73% and 0.85% compared to GBLUP, RF, BayesB and BayesCπ, respectively across all seven traits. Meanwhile, both BANN_100kb and BANN_gene yielded lower overall mean square error values than GBLUP, RF and Bayesian methods.</p><p><strong>Conclusion: </strong>Our findings demonstrated that the BANNs framework performed better than traditional genomic prediction methods in our tested scenarios, and might serve as a promising alternative approach for genomic prediction in dairy cattle.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":null,"pages":null},"PeriodicalIF":6.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11215832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prebiotic galactooligosaccharide improves piglet growth performance and intestinal health associated with alterations of the hindgut microbiota during the peri-weaning period. 益生元半乳寡糖可改善仔猪的生长性能以及与围断奶期后肠微生物群变化相关的肠道健康。
Pub Date : 2024-06-13 DOI: 10.1186/s40104-024-01047-y
Timothy E Boston, Feng Wang, Xi Lin, Sung Woo Kim, Vivek Fellner, Mark F Scott, Amanda L Ziegler, Laurianne Van Landeghem, Anthony T Blikslager, Jack Odle

Background: Weaning stress reduces growth performance and health of young pigs due in part to an abrupt change in diets from highly digestible milk to fibrous plant-based feedstuffs. This study investigated whether dietary galactooligosaccharide (GOS), supplemented both pre- and post-weaning, could improve growth performance and intestinal health via alterations in the hindgut microbial community.

Methods: Using a 3 × 2 factorial design, during farrowing 288 piglets from 24 litters received either no creep feed (FC), creep without GOS (FG-) or creep with 5% GOS (FG+) followed by a phase 1 nursery diet without (NG-) or with 3.8% GOS (NG+). Pigs were sampled pre- (D22) and post-weaning (D31) to assess intestinal measures.

Results: Creep fed pigs grew 19% faster than controls (P < 0.01) prior to weaning, and by the end of the nursery phase (D58), pigs fed GOS pre-farrowing (FG+) were 1.85 kg heavier than controls (P < 0.05). Furthermore, pigs fed GOS in phase 1 of the nursery grew 34% faster (P < 0.04), with greater feed intake and efficiency. Cecal microbial communities clustered distinctly in pre- vs. post-weaned pigs, based on principal coordinate analysis (P < 0.01). No effects of GOS were detected pre-weaning, but gruel creep feeding increased Chao1 α-diversity and altered several genera in the cecal microbiota (P < 0.05). Post-weaning, GOS supplementation increased some genera such as Fusicatenibacter and Collinsella, whereas others decreased such as Campylobacter and Frisingicoccus (P < 0.05). Changes were accompanied by higher molar proportions of butyrate in the cecum of GOS-fed pigs (P < 0.05).

Conclusions: Gruel creep feeding effectively improves suckling pig growth regardless of GOS treatment. When supplemented post-weaning, prebiotic GOS improves piglet growth performance associated with changes in hindgut microbial composition.

背景:断奶应激会降低幼猪的生长性能和健康水平,部分原因是日粮从高消化率的牛奶突然转变为纤维植物性饲料。本研究调查了在断奶前和断奶后补充日粮半乳寡糖(GOS)是否能通过改变后肠微生物群落来改善生长性能和肠道健康:采用 3 × 2 因子设计,在产仔期间,24 胎的 288 头仔猪分别接受不添加爬行饲料(FC)、不添加 GOS 的爬行饲料(FG-)或添加 5% GOS 的爬行饲料(FG+),然后再添加不添加 GOS 的第一阶段保育日粮(NG-)或添加 3.8% GOS 的第一阶段保育日粮(NG+)。猪在断奶前(D22)和断奶后(D31)采样评估肠道指标:结果:匍匐饲喂的猪的生长速度比对照组快 19%(P 结论:匍匐饲喂能有效改善猪的生长速度:无论 GOS 的处理方法如何,稀粥匍匐饲喂都能有效改善乳猪的生长。断奶后补充益生元 GOS 可提高仔猪的生长性能,这与后肠微生物组成的变化有关。
{"title":"Prebiotic galactooligosaccharide improves piglet growth performance and intestinal health associated with alterations of the hindgut microbiota during the peri-weaning period.","authors":"Timothy E Boston, Feng Wang, Xi Lin, Sung Woo Kim, Vivek Fellner, Mark F Scott, Amanda L Ziegler, Laurianne Van Landeghem, Anthony T Blikslager, Jack Odle","doi":"10.1186/s40104-024-01047-y","DOIUrl":"10.1186/s40104-024-01047-y","url":null,"abstract":"<p><strong>Background: </strong>Weaning stress reduces growth performance and health of young pigs due in part to an abrupt change in diets from highly digestible milk to fibrous plant-based feedstuffs. This study investigated whether dietary galactooligosaccharide (GOS), supplemented both pre- and post-weaning, could improve growth performance and intestinal health via alterations in the hindgut microbial community.</p><p><strong>Methods: </strong>Using a 3 × 2 factorial design, during farrowing 288 piglets from 24 litters received either no creep feed (FC), creep without GOS (FG-) or creep with 5% GOS (FG+) followed by a phase 1 nursery diet without (NG-) or with 3.8% GOS (NG+). Pigs were sampled pre- (D22) and post-weaning (D31) to assess intestinal measures.</p><p><strong>Results: </strong>Creep fed pigs grew 19% faster than controls (P < 0.01) prior to weaning, and by the end of the nursery phase (D58), pigs fed GOS pre-farrowing (FG+) were 1.85 kg heavier than controls (P < 0.05). Furthermore, pigs fed GOS in phase 1 of the nursery grew 34% faster (P < 0.04), with greater feed intake and efficiency. Cecal microbial communities clustered distinctly in pre- vs. post-weaned pigs, based on principal coordinate analysis (P < 0.01). No effects of GOS were detected pre-weaning, but gruel creep feeding increased Chao1 α-diversity and altered several genera in the cecal microbiota (P < 0.05). Post-weaning, GOS supplementation increased some genera such as Fusicatenibacter and Collinsella, whereas others decreased such as Campylobacter and Frisingicoccus (P < 0.05). Changes were accompanied by higher molar proportions of butyrate in the cecum of GOS-fed pigs (P < 0.05).</p><p><strong>Conclusions: </strong>Gruel creep feeding effectively improves suckling pig growth regardless of GOS treatment. When supplemented post-weaning, prebiotic GOS improves piglet growth performance associated with changes in hindgut microbial composition.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11170840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141312417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation. 核桃肽 TW-7 可通过促进组蛋白乳酰化改善玻璃化 MII 卵母细胞的小鼠孤雌胚发育。
Pub Date : 2024-06-11 DOI: 10.1186/s40104-024-01045-0
Yaozong Wei, Bo Pan, Jianpeng Qin, Beijia Cao, Tianyi Lv, Jiangfeng Ye, Ao Ning, Kunlin Du, Xiangyi Chen, Shuqi Zou, Shengqin Zang, Guozhi Yu, Tianzeng Song, Qiuxia Liang, Guangbin Zhou

Background: Previous studies have shown that the vitrification of metaphase II (MII) oocytes significantly represses their developmental potential. Abnormally increased oxidative stress is the probable factor; however, the underlying mechanism remains unclear. The walnut-derived peptide TW-7 was initially isolated and purified from walnut protein hydrolysate. Accumulating evidences implied that TW-7 was a powerful antioxidant, while its prospective application in oocyte cryopreservation has not been reported.

Result: Here, we found that parthenogenetic activation (PA) zygotes derived from vitrified MII oocytes showed elevated ROS level and delayed progression of pronucleus formation. Addition of 25 μmol/L TW-7 in warming, recovery, PA, and embryo culture medium could alleviate oxidative stress in PA zygotes from vitrified mouse MII oocytes, furtherly increase proteins related to histone lactylation such as LDHA, LDHB, and EP300 and finally improve histone lactylation in PA zygotes. The elevated histone lactylation facilitated the expression of minor zygotic genome activation (ZGA) genes and preimplantation embryo development.

Conclusions: Our findings revealed the mechanism of oxidative stress inducing repressed development of PA embryos from vitrified mouse MII oocytes and found a potent and easy-obtained short peptide that could significantly rescue the decreased developmental potential of vitrified oocytes, which would potentially contribute to reproductive medicine, animal protection, and breeding.

背景:先前的研究表明,玻璃化的 II 期卵母细胞(MII)会显著抑制其发育潜能。氧化应激异常增加是可能的因素,但其潜在机制仍不清楚。核桃肽 TW-7 最初是从核桃蛋白水解物中分离和纯化出来的。越来越多的证据表明 TW-7 是一种强大的抗氧化剂,但其在卵母细胞冷冻保存中的应用前景尚未见报道:结果:在这里,我们发现从玻璃化的 MII 卵母细胞中获得的孤雌生殖激活(PA)子代表现出 ROS 水平升高和前核形成进展延迟。在温育、复苏、PA和胚胎培养液中添加25 μmol/L TW-7可缓解玻璃化小鼠MII卵母细胞产生的PA子代的氧化应激,进一步增加与组蛋白乳化相关的蛋白质,如LDHA、LDHB和EP300,最终改善PA子代的组蛋白乳化。组蛋白乳化的提高促进了次要子代基因组激活(ZGA)基因的表达和植入前胚胎的发育:我们的研究结果揭示了氧化应激诱导玻璃化小鼠 MII 卵母细胞 PA 胚胎发育受抑制的机制,并发现了一种有效且易于获得的短肽,它能显著挽救玻璃化卵母细胞发育潜能的下降,这将为生殖医学、动物保护和育种做出潜在贡献。
{"title":"The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation.","authors":"Yaozong Wei, Bo Pan, Jianpeng Qin, Beijia Cao, Tianyi Lv, Jiangfeng Ye, Ao Ning, Kunlin Du, Xiangyi Chen, Shuqi Zou, Shengqin Zang, Guozhi Yu, Tianzeng Song, Qiuxia Liang, Guangbin Zhou","doi":"10.1186/s40104-024-01045-0","DOIUrl":"10.1186/s40104-024-01045-0","url":null,"abstract":"<p><strong>Background: </strong>Previous studies have shown that the vitrification of metaphase II (MII) oocytes significantly represses their developmental potential. Abnormally increased oxidative stress is the probable factor; however, the underlying mechanism remains unclear. The walnut-derived peptide TW-7 was initially isolated and purified from walnut protein hydrolysate. Accumulating evidences implied that TW-7 was a powerful antioxidant, while its prospective application in oocyte cryopreservation has not been reported.</p><p><strong>Result: </strong>Here, we found that parthenogenetic activation (PA) zygotes derived from vitrified MII oocytes showed elevated ROS level and delayed progression of pronucleus formation. Addition of 25 μmol/L TW-7 in warming, recovery, PA, and embryo culture medium could alleviate oxidative stress in PA zygotes from vitrified mouse MII oocytes, furtherly increase proteins related to histone lactylation such as LDHA, LDHB, and EP300 and finally improve histone lactylation in PA zygotes. The elevated histone lactylation facilitated the expression of minor zygotic genome activation (ZGA) genes and preimplantation embryo development.</p><p><strong>Conclusions: </strong>Our findings revealed the mechanism of oxidative stress inducing repressed development of PA embryos from vitrified mouse MII oocytes and found a potent and easy-obtained short peptide that could significantly rescue the decreased developmental potential of vitrified oocytes, which would potentially contribute to reproductive medicine, animal protection, and breeding.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1. UCHL1 通过稳定 CCNB1 促进猪颗粒细胞的增殖。
Pub Date : 2024-06-11 DOI: 10.1186/s40104-024-01043-2
Shengjie Shi, Huan Yuan, Lutong Zhang, Lei Gao, Lili Zhao, Xiangfang Zeng, Shiyan Qiao, Guiyan Chu, Chuanjiang Cai

Background: The proliferation of porcine ovarian granulosa cells (GCs) is essential to follicular development and the ubiquitin-proteasome system is necessary for maintaining cell cycle homeostasis. Previous studies found that the deubiquitinase ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) regulates female reproduction, especially in ovarian development. However, the mechanism by which UCHL1 regulates porcine GC proliferation remains unclear.

Results: UCHL1 overexpression promoted GC proliferation, and knockdown had the opposite effect. UCHL1 is directly bound to cyclin B1 (CCNB1), prolonging the half-life of CCNB1 and inhibiting its degradation, thereby promoting GC proliferation. What's more, a flavonoid compound-isovitexin improved the enzyme activity of UCHL1 and promoted the proliferation of porcine GCs.

Conclusions: UCHL1 promoted the proliferation of porcine GCs by stabilizing CCNB1, and isovitexin enhanced the enzyme activity of UCHL1. These findings reveal the role of UCHL1 and the potential of isovitexin in regulating proliferation and provide insights into identifying molecular markers and nutrients that affect follicle development.

背景:猪卵巢颗粒细胞(GCs)的增殖对卵泡的发育至关重要,而泛素-蛋白酶体系统对维持细胞周期的平衡十分必要。以前的研究发现,去泛素酶泛素羧基末端水解酶1(UCHL1)调节女性生殖,尤其是卵巢发育。然而,UCHL1调控猪GC增殖的机制仍不清楚:结果:UCHL1过表达可促进GC增殖,而敲除则产生相反的效果。UCHL1直接与细胞周期蛋白B1(CCNB1)结合,延长CCNB1的半衰期并抑制其降解,从而促进GC增殖。此外,黄酮类化合物异黄酮素提高了UCHL1的酶活性,促进了猪GC的增殖:结论:UCHL1通过稳定CCNB1促进了猪GCs的增殖,而异维甲酸素提高了UCHL1的酶活性。这些发现揭示了UCHL1在调节增殖中的作用和异维甲酸素的潜力,并为确定影响卵泡发育的分子标记和营养物质提供了启示。
{"title":"UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1.","authors":"Shengjie Shi, Huan Yuan, Lutong Zhang, Lei Gao, Lili Zhao, Xiangfang Zeng, Shiyan Qiao, Guiyan Chu, Chuanjiang Cai","doi":"10.1186/s40104-024-01043-2","DOIUrl":"10.1186/s40104-024-01043-2","url":null,"abstract":"<p><strong>Background: </strong>The proliferation of porcine ovarian granulosa cells (GCs) is essential to follicular development and the ubiquitin-proteasome system is necessary for maintaining cell cycle homeostasis. Previous studies found that the deubiquitinase ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) regulates female reproduction, especially in ovarian development. However, the mechanism by which UCHL1 regulates porcine GC proliferation remains unclear.</p><p><strong>Results: </strong>UCHL1 overexpression promoted GC proliferation, and knockdown had the opposite effect. UCHL1 is directly bound to cyclin B1 (CCNB1), prolonging the half-life of CCNB1 and inhibiting its degradation, thereby promoting GC proliferation. What's more, a flavonoid compound-isovitexin improved the enzyme activity of UCHL1 and promoted the proliferation of porcine GCs.</p><p><strong>Conclusions: </strong>UCHL1 promoted the proliferation of porcine GCs by stabilizing CCNB1, and isovitexin enhanced the enzyme activity of UCHL1. These findings reveal the role of UCHL1 and the potential of isovitexin in regulating proliferation and provide insights into identifying molecular markers and nutrients that affect follicle development.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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