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Preliminary Study Regarding the Additional Effect of Adding Antioxidants on Bull Frozen Semen 添加抗氧化剂对公牛冷冻精液补充作用的初步研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0067
Kassem Houshaimy, D. Țogoe, T. Constantin, C. Micsa, A. Șonea
Abstract For a long period of time, cryopreservation and dilution of sperm represented a very important role in improvement of biotechnology of artificial insemination in the bovine. Regarding to this fact the study has been realized in period 2016- 2017 at the center of amelioration for seminal material, Semtest-BVN Târgu-Mureș and at the laboratory of theriogenology of Faculty of veterinary medicine Bucharest. In this study, 7 ml sperm from one Holstein bull (SunnyBoy) was obtained using artificial vagina. The ejaculate was divided to 5 parts and diluted using Triladyl extender (250 g) adding vitamin E with 2 concentrations 1μmol or 2 μmol, catechin 0.1μmol, Trolox 0.1 μmol, and a standard probe without adding antioxidants. Straws had been analyzed using Computer- assisted sperm analysis (CASA) for motility and composite after thawing. As a first conclusion of this study, it can say that the group with vitamin E presented a higher motility than the other groups.
长期以来,精子的冷冻和稀释在牛人工授精的生物技术改进中起着非常重要的作用。鉴于这一事实,该研究于2016年至2017年期间在种子材料改良中心Semtest-BVN t rgu- murebus和布加勒斯特兽医学院兽医学实验室完成。在本研究中,使用人工阴道从一头荷斯坦公牛(SunnyBoy)获得7ml精子。将射精液分成5份,用三甘醇扩展剂(250 g)稀释,分别添加2种浓度的维生素E (1μmol或2 μmol)、儿茶素0.1μmol、Trolox 0.1μmol,标准探针不添加抗氧化剂。用计算机辅助精子分析(CASA)分析了秸秆解冻后的活力和复合成分。作为本研究的第一个结论,可以说维生素E组比其他组表现出更高的运动性。
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引用次数: 0
Histological Assessment of an Experimental Model of Human Osteomyelitis in Rabbits 兔人骨髓炎实验模型的组织学评价
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0065
C. Coman, C. Surdu-Bob, F. Bărbuceanu, E. Vlase, M. Badulescu
Abstract The aim of this paper was to present a histological evaluation of the treatment performed on a humanmodel of osteomyelitis induced in rabbits. Osteomyelitis was induced in rabbits by a human strain of Staphylococcus aureus injected in bone defects created in the rabbit tibia. There have been created five groups of animals, groups receiving treatment in two different stages of the disease, acute and chronic. Copper and silver sub-millimetre-particles were introduced in the same place with the Staphylococcus solution. Evaluation of installation and evolution of the disease was done by clinical, hematological, microbiological, radiological and histological monitoring. A separate study of histological data is presented here. Histological examinations performed by HE was done on 5 μm sections of uncalcified bone. Every examination was classified according to a system of score. The results of histopathological evaluation confirm that histological analysis is a powerful tool in experimental models of this disease.
摘要:本文的目的是提出一个组织学评价的治疗进行了人源性骨髓炎兔模型。将一种人类金黄色葡萄球菌菌株注射到兔胫骨骨缺损中诱导兔骨髓炎。已经创建了五组动物,每组在疾病的两个不同阶段接受治疗,急性和慢性。将亚毫米级的铜和银颗粒与葡萄球菌溶液放在同一位置。通过临床、血液学、微生物学、放射学和组织学监测来评估疾病的发生和发展。这里提出了一项单独的组织学数据研究。对未钙化骨5 μm切片进行HE组织学检查。每次考试都按分数制度进行分类。组织病理学评估结果证实,组织病理学分析是该疾病实验模型的有力工具。
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引用次数: 1
Estimates of the Population Parameters and Exploitation Rate of Pontic Shad (Alosa Immaculata Bennett, 1835) in the Romanian Black Sea Coast 罗马尼亚黑海沿岸庞蒂克鲥鱼种群参数和捕捞率的估计(Alosa Immaculata Bennett, 1835
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0024
G. Țiganov, M. Nenciu, C. Danilov, V. Niță
Abstract Sex ratio, morphometric characteristics, age and growth for Pontic shad from Romanian Black Sea Coast were examined. A total of 2.133 individuals were caught between March 2012 and September 2013. Female : male sex ratio was 0.62. According to the age reading, distribution varied form II to V year. The von Bertalanffy equation and growth performance index were determined by ESP software in Pontic shad. The asymptotic length (L∞ = L infinity) of Pontic shad generated by the ELEFAN I method, after introducing total length data for the two years of study, was 41.5. The natural mortality instantaneous coefficient (M) of the entire Pontic shad population, calculated according to Pauly’s empirical equation, using the growth parameters of the Von Bertalanffy formula and the mean annual temperature of the two study years of 13°C, recorded the following values: M = 0.585 in 2012 and M = 0.639 in 2013. According with the resultants, it is recommended to perform a sustainable fishing which allows the capture of legal-sized of fish.
摘要对罗马尼亚黑海沿岸庞蒂鱼的性别比、形态特征、年龄和生长进行了研究。2012年3月至2013年9月期间,共有2.133人被抓获。男女性别比为0.62。根据阅读年龄的不同,分布在二至五年级。用ESP软件确定了庞蒂克沙鱼的von Bertalanffy方程和生长性能指标。引入两年研究的总长度数据后,ELEFAN I方法生成的Pontic曲线的渐近长度(L∞= L∞)为41.5。根据保利经验方程,利用Von Bertalanffy公式的生长参数和两个研究年的年平均温度13℃,计算出整个庞tic鲱鱼种群的自然死亡瞬时系数M,得到的值为:2012年M = 0.585, 2013年M = 0.639。根据研究结果,建议实行可持续捕捞,允许捕获法定大小的鱼类。
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引用次数: 2
Camelina Sativa Crop-Instrument for Phytoremediation or Safe Culture Grown on Contaminated Soil? 亚麻荠作物——在污染土壤上进行植物修复或安全栽培的工具?
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0089
A. Popa, V. Drumea, M. Florea, L. Olariu, Ș. Jurcoane
Abstract The decontamination and detoxification of pesticides-contaminated soils is a tough task, due to their persistence and mobility. Camelina sativa is resistant to pests and weeds which affect similar crops mostly due to its unique defence system, attributed tocamalexin and metoxi-camalexin. Considering this, camelina may be cultivated without input of pesticides, but what happens if camelina is cultivated on an already pesticide-contaminated soil? The purpose of the study is to analyze the Camelina sativa potential to concentrate or not the residual pesticides from the soil in its seeds.It was assessed the level of pesticides from the soil on which Camelina sativa-Mădălina variety was cultivated and from the resulted seeds. The quantification was done using a GC-MS-MS equipment. In the soil it was found a total amount of 0.03 mg/kg of pesticides consisting in: o,p-DDE, p,p-DDE, o,p-DDD, o,p-DDT, p,p-DDT, from which 81.4% was p,p-DDE. All the signals registered for camelina seeds were below the LOQ of 0.005mg/kg. Camelina sativa-Mădălina variety can be cultivated on soils contaminated with DDT and its metabolites, minimizing the risk of pesticides translocation.
由于农药污染土壤的持久性和流动性,农药污染土壤的净化和脱毒是一项艰巨的任务。亚麻荠对影响同类作物的害虫和杂草具有抗性,主要是由于其独特的防御系统,归因于camalexin和metox -camalexin。考虑到这一点,亚麻荠可以在没有农药的情况下种植,但如果亚麻荠种植在已经被农药污染的土壤上,会发生什么呢?本研究的目的是分析亚麻荠在种子中浓缩或不浓缩土壤中残留农药的潜力。对栽培亚麻荠-麻籽荠的土壤和种子的农药残留量进行了评价。定量采用气相色谱-质谱联用仪。土壤中农药残留量为0.03 mg/kg,由:0、p- dde、p、p- dde、0、p- ddd、0、p- ddt、p、p- ddt组成,其中p、p- dde占81.4%。所有检测到的信号均低于0.005mg/kg的定量限。亚麻荠-麻籽荠品种可以在被DDT及其代谢物污染的土壤上种植,最大限度地减少农药转运的风险。
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引用次数: 1
Effects of Combined Application of Bradyrhizobium Japonicum and Pseudomonas Putida on Nutrients and Water Contents of Soybean in Relation to Soil Moisture Regime 缓生根瘤菌与恶臭假单胞菌配施对大豆养分和水分的影响与土壤水分状况的关系
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0016
V. Rotaru
Abstract Plant growth promoting rhizobacteria (PGPR) are of growing interest for their potential role in improving soil fertility and enhancing crops productivity and nutrients contents. In order to assess the impact of PGPR on nutrients contents of soybean, a greenhouse experiment was carried out with Bradyrhizobium japonicum applied alone or in combination with Pseudomonas putida in conjunction with fertilization of P or manure. Two soil moisture regimes were installed: 70% of whole holding capacity (WHC) as control and 35% WHC as moderate water stress. The water deficit was imposed at the flowering stage for 12 days. Plants cultivated under P insufficiency and drought exhibited lower physiological parameters in treatment with single inoculation of B. japonicum than combined application of two rhizobacteria. The results showed that the combined application of rhizobacteria enhanced the N and P contents of soybean regarding of soil water conditions. The integrated use of rhizobacteria improved the relative water content of soybean. Their influence was more pronounced under P deficiency and manure fertilization of plants especially under no stress water conditions. Hence, combined application of B. japonicum and P. putida has a potential to improve nutrition and growth of soybean under normal soil moisture as well as under moderate drought.
促进植物生长的根瘤菌(rhizobacteria, PGPR)因其在改善土壤肥力、提高作物生产力和养分含量方面的潜在作用而受到越来越多的关注。为评价PGPR对大豆养分含量的影响,在大棚试验中,进行了缓生根瘤菌单独施用或与恶臭假单胞菌联合施用,同时施磷肥或粪肥的试验。设置了两种土壤水分状态:70%的总保有量为控制,35%的总保有量为适度水分胁迫。在花期施用水分亏缺12天。在缺磷和干旱条件下栽培的植株,单次接种日本芽孢杆菌比两种根瘤菌联合施用表现出较低的生理参数。结果表明,在不同土壤水分条件下,根菌配施提高了大豆氮、磷含量。根瘤菌的综合利用提高了大豆的相对含水量。其影响在缺磷和施肥条件下更为明显,特别是在无胁迫水分条件下。因此,在正常土壤湿度和中度干旱条件下,日本芽孢杆菌和腐殖霉配施对大豆的营养和生长都有改善作用。
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引用次数: 1
Researches Concerning Sunflower Seeds Treatment against Tanymecus Dilaticollis, in Green House Conditions 向日葵种子在温室条件下对扩张斑蝽的处理研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0010
E. Georgescu, M. Toader, A. Ionescu, L. Cană, L. Râşnoveanu
Abstract In the last years sunflower become one of the most important crops in Romania. The most of the surface cultivated with sunflower from our country is located in south and south-east and, in many cases, sunflower is cultivated after maize. As result the attack of the maize leaf weevil (Tanymecus dilaticollis Gyll) at sunflower plants has increasing. The attack is dangerous when sunflower plants are in first vegetation stages (BBCH 10-BBCH 14). Similar with maize crop, sunflower seeds treatment with neonicotinoids were generalized after 2007. As result of the EU directive 485/2013, no active ingredients remain available for sunflower seeds treatment, against T. dilaticollis, in Romania. The researches from the last years were orientated for finding of the possible alternatives for seed treatments, in eventuality of permanent ban of neonicotinoid insecticides, after 2018. Because of the variability of climatic conditions from the field, during spring period, researches concerning effectiveness testing of sunflower seed treatments, in green house conditions, using high pest pressure is a good method to evaluate influence of the different active ingredients to control T. dilaticollis adults. In this experiment it has tested three doses of cyantraniliprole active ingredient (7.5, 10.0 and 12.5 l/to) and three neonicothinoid active ingredients (imidacloprid, clothianidin, thiamethoxam). Sunflower seeds were placed in plastic pots. After plants emergence the insects were added in pots. At each pot it has added 20 adult insects, collected from the field. Each day, it has recorded insect mortality. At 8 days from the start of the experiment, it has assessed attack intensity of the adult insects on sunflower plants. The attack was evaluated using a scale from 1 (plant not attacked) to 9 (plant complete destroyed). In conditions of high pest pressure (4 insects/plant) the attack intensity of T. dilaticollis at sunflower plants was ranged from 3.35 in case of variant treated with clothianidin and 8.50 in case of untreated plants. Lower attack intensity of the pest at sunflower plants it has registered in case of variants treated with imidacloprid and thiamethoxam (I=3.58 and 3.50). Plants emerged from the untreated seeds were destroyed by the insects, at 8 days from the start of the experiment.
近年来,向日葵成为罗马尼亚最重要的农作物之一。我国种植向日葵的地表大部分位于南部和东南部,在许多情况下,向日葵是在玉米之后种植的。因此,玉米叶象鼻虫(Tanymecus dilaticollis Gyll)对向日葵植株的侵害有所增加。当向日葵植物处于第一植被阶段(BBCH 10-BBCH 14)时,这种攻击是危险的。与玉米作物类似,葵花籽新烟碱处理在2007年后得到推广。根据欧盟第485/2013号指令,在罗马尼亚,葵花籽治疗中没有有效成分可用。过去几年的研究旨在寻找种子处理的可能替代品,以期在2018年之后永久禁用新烟碱类杀虫剂。由于田间气候条件的多变性,在春季进行向日葵种子处理的有效性试验研究,在温室条件下,采用高害虫压力是评价不同有效成分对大尺蠖成虫防治效果的较好方法。本实验测试了三种剂量的氰胺虫胺活性成分(7.5、10.0和12.5 l/to)和三种新烟碱类活性成分(吡虫啉、噻虫胺、噻虫嗪)。葵花籽被放在塑料盆里。植物出苗后,将昆虫放入花盆中。每个花盆里都有20只从田间采集的成虫。每天都记录昆虫的死亡率。试验开始后第8天,评估成虫对向日葵植株的攻击强度。攻击的评估范围从1(植物未被攻击)到9(植物完全被摧毁)。在高虫害压力(4虫/株)条件下,施虫胺处理的向日葵植株上,膨胀小蠹的攻击强度为3.35,未施虫胺处理的向日葵植株上,膨胀小蠹的攻击强度为8.50。用吡虫啉和噻虫嗪处理的变种在向日葵植株上的攻击强度较低(I=3.58和3.50)。在实验开始后的第8天,从未经处理的种子中生长出来的植物被昆虫破坏。
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引用次数: 3
Human Health Risks Associated with Chemical and Mycrobiological Contaminants in Fish - A Mini Review 与鱼类中化学和微生物污染物相关的人类健康风险-综述
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0063
A. Cioca, I. Balla, M. Mihaiu, L. Colobatiu
Abstract Fish meat consumption has gained a lot of popularity in Europe in the last years. It is considered to be a great alternative for red meat because it provides large amounts of Omega-3 fatty acids and vitamin D. These components lower the risk of cardiovascular disease, reduce the risk of autoimmune diseases, are essential for growth and development, can contribute to mood elevation and can prevent cognitive decline, and eye disease. Unfortunately the benefits can sometimes be accompanied by a risk associated with chemical and microbiological contaminants. The aim of this paper is to review some recently published studies concerning the groups of chemical compounds and bacteria found in fish meat, which can have negative effects on human health. In order to raise awareness, aspects related to dangerous persistent organic pollutants and zoonotic bacteria are described. The frequency of encountering them is discussed, the ways in which they can reach the human body are specified and a critical comparison of their consequences is performed.
近年来,鱼肉消费在欧洲越来越受欢迎。它被认为是红肉的一个很好的替代品,因为它提供了大量的Omega-3脂肪酸和维生素d。这些成分可以降低患心血管疾病的风险,降低患自身免疫性疾病的风险,对生长发育至关重要,可以促进情绪提升,防止认知能力下降和眼病。不幸的是,这些好处有时伴随着与化学和微生物污染物相关的风险。本文的目的是回顾最近发表的一些关于在鱼肉中发现的对人体健康有负面影响的化合物和细菌群的研究。为了提高人们的认识,介绍了与危险的持久性有机污染物和人畜共患细菌有关的方面。讨论了遇到它们的频率,规定了它们可以到达人体的方式,并对它们的后果进行了关键的比较。
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引用次数: 0
Researches Concerning the Influence of Technological Links on Dendrometric Parameters to Paulownia Ssp. 工艺环节对泡桐树学参数影响的研究。
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0043
R. Dobrinoiu, S. Dănăilă-Guidea, R. Ivan, C. Filip, Fabio Massimo Sprio
Abstract Paulownia ssp., in its places of origin, has been cultivated for over 2600 years and used as protection curtains against sand storms or floods, studies of how this species was exploited only fifty years ago in time what genetic research has only been affected in the last thirty years. The fundamental purpose of experimentation was to check the influence of different technological links (planting density and fertilization scheme), not only on the weight of the wood and the technological characteristics of the wood obtained, but also on the total biomass accumulation. The objectives of the research were: a) stems diameter increase, measured at 100 cm height from the soil surface, maintaining the harvest variant at 4 years; b) increasing of biomass accumulation. In all experimental variants, it is noted that the plants recorded higher values of the different variables analyzed (diameter at the base, normal diameter and total height), when the fertilization factor also intervenes. In the same way, when a higher fertilization was applied, higher values of the dendrometric indicators are obtained compared to the experimental variants that did not benefit from the supplementation of nutrients, suggesting that the most important risk factor in Paulownia plantations is the planting density and the fertilization element.
泡桐属植物。在它的起源地,它已经被种植了2600多年,被用作抵御沙尘暴或洪水的屏障,关于这个物种是如何被利用的研究只是在50年前,而基因研究只是在最近30年才受到影响。试验的根本目的是检查不同的技术环节(种植密度和施肥方案)不仅对木材重量和所获得木材的技术特性的影响,而且对总生物量积累的影响。研究的目的是:a)茎粗增加,在距土壤表面100 cm高度测量,保持4年的收获变化;B)生物量积累增加。在所有的试验变异体中,当施肥因素也干预时,植株的不同变量(基部直径、正常直径和总高)的值都较高。同样,当施用较高的肥料时,树木指标的数值高于未受益于营养补充的试验变异,这表明泡桐人工林最重要的危险因素是种植密度和施肥元素。
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引用次数: 0
The Effects of Phosphorous and Selenium Treatments on Arsenic Uptake and Plant Growth in Rice (Oryza sativa L.) 磷和硒处理对水稻砷吸收及植株生长的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0001
J. Barton, R. Collins, A. Westland, L. Butters
Abstract Phosphorus (P) and Selenium (Se) supplementation to rice plants grown in Arsenic (As) contaminated conditions as be found by many studies to reduce As uptake and benefit growth in such conditions, however there are some inconsistencies as to how effective these treatments are. This study investigates the effect of 0, 0.5, 1, 1.5 and double the recommended concentration of P and Se on the growth of rice seedlings both with and without the presence of As over a maximum 20 day period. Analysis of the growth data collected indicated that there is no significant difference in the leaf, maximum and minimum root lengths, leaf and root numbers or the As content of the plant material. This study finds that different concentrations of P and Se do not affect growth at early stages and do not affect As uptake.
许多研究发现,在砷污染条件下,水稻植株补充磷(P)和硒(Se)可以减少砷的吸收,有利于生长,但这些处理的效果如何却存在一些不一致。本研究研究了0、0.5、1、1.5和2倍推荐浓度的磷和硒对有砷和无砷水稻幼苗生长的影响,时间最长为20 d。对收集到的生长数据进行分析表明,叶片、最大最小根长、叶片和根数以及植株材料中砷含量无显著差异。本研究发现,不同浓度的磷和硒不影响水稻早期生长,也不影响水稻对砷的吸收。
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引用次数: 1
Long-Term Tillage Induced Changes in Physical Attributes of a Clayey Soil in Eastern Mediterranean Region 长期耕作对东地中海地区粘性土物理特性的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0005
İ. Çelik, H. Günal, M. Acar, Zeliha Barut Bereket, N. Acir, M. Budak
Abstract Comparing responses of the same soil under long-term different treatments is vital in determining the best management practices to conserve soil health and sustain productivity. This study was investigated to determine the effects of longterm (2006-2015) two conventional (CT), three reduced (RT) and a no-till (NT) treatment on bulk density (BD), field capacity (FC), wilting point (WP), available water content (AWC) and total porosity (TP) of a clayey soil in eastern Mediterranean region, Turkey. Undisturbed soil samples were collected from 0-10, 10-20 and 20-30 cm depths of experimental plots with a crop rotation of winter wheat (Triticum aestivum L.), soybean (Glycine max. L.), grain maize (Zea mays L.). The AWC under CT was higher than the RT and NT. The BD was increased with depth in all treatments, and was higher under RT and NT than the CT. The long-term experiment revealed that BD increase under long-term RT and NT should be monitored to avoid deterioration of soil health, and yield loss due to limiting root penetration and storing available water needed during drought periods.
比较同一土壤在长期不同处理下的响应对于确定最佳管理措施以保持土壤健康和维持生产力至关重要。本研究旨在确定长期(2006-2015年)2次常规(CT)、3次还原(RT)和1次免耕(NT)处理对土耳其地中海东部地区粘性土容重(BD)、田间容量(FC)、萎蔫点(WP)、有效含水量(AWC)和总孔隙度(TP)的影响。采用冬小麦(Triticum aestivum L.)、大豆(Glycine max.)和小麦(Triticum aestivum L.)轮作,在试验田0 ~ 10、10 ~ 20和20 ~ 30 cm深度采集原状土壤样品。玉米(Zea mays L.)。CT下AWC均高于RT和NT处理,BD随深度增加而增加,且RT和NT处理均高于CT处理。长期试验表明,应监测长期RT和NT下土壤含水量的增加,以避免土壤健康恶化,以及由于限制根系渗透和储存干旱期所需有效水分而造成的产量损失。
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引用次数: 1
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