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Effects of Purified Indian Cattle Tick Rhipicephalus microplus Saliva Toxins on Various Enzymes in Blood Serum, Liver and Muscles of Albino Mice 纯化的印度牛蜱微鼻头加唾液毒素对白化小鼠血清、肝脏和肌肉中各种酶的影响
Pub Date : 2023-01-01 DOI: 10.4236/aer.2023.112005
N. Yadav, R. Upadhyay
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引用次数: 0
Toxic Effects of Citrus maxima Based Combinatorial Formulations on Important Metabolic Enzymes in Indian White Termite Odontotermes obesus 柑桔复合制剂对印度白蚁重要代谢酶的毒性影响
Pub Date : 2023-01-01 DOI: 10.4236/aer.2023.112002
L. Pandey, R. Upadhyay
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引用次数: 0
Toxic Effects of Ficus benghalensis Latex Based Combinatorial Formulations on Various Enzymatic Parameters in Indian White Termite Odontotermes obesus 榕树胶乳组合制剂对印度白蚁各种酶参数的毒性影响
Pub Date : 2023-01-01 DOI: 10.4236/aer.2023.112003
A. Tripathi, R. Upadhyay
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引用次数: 1
Combinatorial Enzyme Approach to Convert Wheat Insoluble Arabinoxylan to Bioactive Oligosaccharides 组合酶法将小麦不溶性阿拉伯木聚糖转化为生物活性低聚糖
Pub Date : 2023-01-01 DOI: 10.4236/aer.2023.111001
D. Wong, S. Batt, William H. Orts
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引用次数: 0
Effect of Purified Paper Wasp Ropalidia marginata Venom Toxin Enzyme Activity in Blood Serum, Liver, and Gastrocnemius Muscle of Albino Mice 纯化纸胡蜂毒毒素酶活性对白化小鼠血清、肝脏和腓肠肌的影响
Pub Date : 2023-01-01 DOI: 10.4236/aer.2023.113006
Simran Sharma, R. Upadhyay
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引用次数: 0
Toxicity of Tagetes erecta Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite Odontotermes obesus 万寿草精油组合制剂对印度白蚁各种代谢酶的毒性研究
Pub Date : 2023-01-01 DOI: 10.4236/aer.2023.112004
Susheel Kumar, R. Upadhyay
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引用次数: 0
Optimization of Growth Conditions to Identify the Superior Bacillus Strain Which Produce High Yield of Thermostable Alpha Amylase 高产耐热淀粉酶芽孢杆菌的生长条件优化
Pub Date : 2022-01-01 DOI: 10.4236/aer.2022.101001
Witharanage Wasana Prasadini Rodrigo, Lakmi Samodha Magamulla, Morawakage Sajith Thiwanka, Yapa Mudiyanselage Shashika Madhuwanthi Yapa
Thermostable α-amylases hold a very important place in commercial industrial applications in Sri Lanka. Therefore, the main aim of this study was to identify superior Bacillus strain and optimize growth conditions that could yield high α-amylase production. Three Bacillus strains, B. amyloliquefaciens ATCC 23350, B. licheniformis ATCC 14580 and B. megaterium ATCC 14581 were used for the study. Shake flask culture experiments were conducted to identify the effect of various fermentation conditions such as growth temperature, incubation period, carbon source, nitrogen source, initial pH and carbon concentration on extracellular α-amylase production. DNSA assay was carried out to determine the enzyme activity. The highest temperature for enzyme activity was reported by B. licheniformis at 85˚C, followed by B. amyloliquefaciens at 75˚C and B. megaterium at 45˚C. Both B. amyloliquefaciens and B. licheniformis were able to give their optimum enzyme production at 37˚C, while B. megaterium at 30˚C in 150 rpm with initial pH of 7. B. licheniformis and B. amyloliquefaciens gave their optimum yield of the enzyme after 48 h of incubation while B. megaterium gave after 24 h of incubation. Among the carbon sources tested cassava starch was able to give the highest enzyme three show high amylase above optimum carbon concentrations according to the Tukey pairwise comparison under ANOVA test than any other carbon concentration. It was observed that gradual increase in extracellular amylase activity up to the optimum and then it was gradually decreased.
耐热α-淀粉酶在斯里兰卡的商业工业应用中占有非常重要的地位。因此,本研究的主要目的是鉴定出优良的芽孢杆菌菌株,并优化其生长条件,使其高产α-淀粉酶。以解淀粉芽孢杆菌ATCC 23350、地衣芽孢杆菌ATCC 14580和巨芽孢杆菌ATCC 14581 3株芽孢杆菌为研究对象。通过摇瓶培养实验,考察生长温度、培养时间、碳源、氮源、初始pH和碳浓度等不同发酵条件对胞外α-淀粉酶产量的影响。采用脱氧核糖核酸(DNSA)测定酶活性。地衣芽孢杆菌的最高酶活温度为85℃,解淀粉芽孢杆菌的最高酶活温度为75℃,megaterium芽孢杆菌的最高酶活温度为45℃。解淀粉芽孢杆菌和地衣芽孢杆菌在37℃条件下产酶最佳,而巨芽孢杆菌在30℃、150 rpm、初始pH为7条件下产酶最佳。地衣芽孢杆菌和解淀粉芽孢杆菌的最佳产酶时间为48 h,而巨芽孢杆菌的最佳产酶时间为24 h。单因素方差分析(ANOVA)下的Tukey两两比较表明,在各碳源中,木薯淀粉在最佳碳浓度以上能产生最高的淀粉酶3个,高于其他碳浓度。观察到细胞外淀粉酶活性逐渐升高,达到最优后逐渐降低。
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引用次数: 1
Studies on Properties of Lipase Produced from Aspergillus sp. Isolated from Compost Soil 堆肥土中分离曲霉产脂肪酶性质的研究
Pub Date : 2022-01-01 DOI: 10.4236/aer.2022.102003
C. O. Ezenwelu, Oladejo A. Afeez, O. U. Anthony, Okoro A. Promise, Ude-Ezika C. Mmesoma, Oparaji E. Henry
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引用次数: 0
Molecular Docking Studies of Estrone-Coumarin Derivatives as Aromatase and 17β-HSD1 Inhibitors Related to Hormone Receptor Positive (HR+) Breast Cancer 雌激素-香豆素衍生物作为激素受体阳性(HR+)乳腺癌相关芳香化酶和17β-HSD1抑制剂的分子对接研究
Pub Date : 2022-01-01 DOI: 10.4236/aer.2022.104006
Silvia Alejandra Meza-Ireta, Blanca Colín-Lozano, Penélope Merino-Montiel, José Luis Vega-Báez, S. Montiel-Smith
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引用次数: 0
Microbial Enzymes and Their Applications in Food Industry: A Mini-Review 微生物酶及其在食品工业中的应用综述
Pub Date : 2022-01-01 DOI: 10.4236/aer.2022.101002
M. Okpara
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引用次数: 16
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