Objective To study the relationship between chemotherapeutic resistance to 5-fluorouracil (5-FU) and Golgi phosphoprotein 3 (GOLPH3) expression in human colon cancer cell line HT29. Methods HT29 cells were divided into four groups: control group, siRNA transfection group, experimental group 1(30 μmol/L 5-FU), and experimental group 2(siRNA-GOLPH3+ 30 μmol/L 5-FU). The silencing effect of GOLPH3 gene was detected by RT-PCR and Western blot. The tumor proliferation and formation ability of HT29 cell were measured respectively by MTT and plate colony formation assay. The protein expressions of GOLPH3, P-gp and β-catenin in HT29 cell were detected by Western blot. Results Compared with the control group, the expression level of GOLPH3 mRNA(1.000±0.078 vs. 0.147±0.021, t=12.296, P<0.01) and protein(1.003±0.235 vs. 0.077±0.399, t=6.723, P<0.01), the absorbance(A value) (1.000±0.082 vs. 0.769±0.086, t=3.885, P<0.01) and the tumorigenesis(430±36 vs. 297±21, t=5.492, P<0.01) in transfection group significantly reduced. The A value(0.803±0.101 vs. 1.050±0.140, t=2.855, P<0.05; 0.242±0.091 vs. 1.050±0.140, t=9.664, P<0.001) and the tumorigenesis(73±8 vs. 427±29, t=20.363, P<0.001; 305±22 vs .427±29, t=5.840, P<0.01) in experimental group 1 and experimental group 2 were significantly lower than that in the control group, while the A value(t=8.264, P<0.001) and the tumorigenesis(t=17.346, P<0.001) of experimental group 2 significantly decreased than that experimental group 1. Compared to the control group(0.967±0.094, 1.001±0.199, 1.000±0.101), the expressions of GOLPH3, P-gp and β-catenin in experimental group 1(3.634±0.574, 2.424±0.261, 2.324±0.418) significantly upregulated(t=7.944, P<0.01; t=7.520, P<0.01; t=5.330, P<0.01), while the expressions of these proteins in experimental group 2(0.520±0.176, 0.466±0.159, 0.933±0.049) were significantly lower than that in experimental group 1(t=8.983, P<0.01; t=11.106, P<0.001; t=5.724, P<0.01). Conclusion Expression of GOLPH3 is associated with resistance to 5-FU in colon cancer HT29 cell by activating Wnt/β-catenin signal pathway. Key words: Colonic neoplasms; Fluorouracil; Wnt signaling pathway; P-Glycoprotein
{"title":"The effect of GOLPH3 expression on chemotherapeutic resistance to 5-Fluorouracil in human colon cancer cell lines","authors":"Hai-Bin Zhuang, Chengzhi Qiu, Ming-Zhen Wang, Chunxiao Wang, Zhi-xiong Chen","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.08.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.08.016","url":null,"abstract":"Objective \u0000To study the relationship between chemotherapeutic resistance to 5-fluorouracil (5-FU) and Golgi phosphoprotein 3 (GOLPH3) expression in human colon cancer cell line HT29. \u0000 \u0000 \u0000Methods \u0000HT29 cells were divided into four groups: control group, siRNA transfection group, experimental group 1(30 μmol/L 5-FU), and experimental group 2(siRNA-GOLPH3+ 30 μmol/L 5-FU). The silencing effect of GOLPH3 gene was detected by RT-PCR and Western blot. The tumor proliferation and formation ability of HT29 cell were measured respectively by MTT and plate colony formation assay. The protein expressions of GOLPH3, P-gp and β-catenin in HT29 cell were detected by Western blot. \u0000 \u0000 \u0000Results \u0000Compared with the control group, the expression level of GOLPH3 mRNA(1.000±0.078 vs. 0.147±0.021, t=12.296, P<0.01) and protein(1.003±0.235 vs. 0.077±0.399, t=6.723, P<0.01), the absorbance(A value) (1.000±0.082 vs. 0.769±0.086, t=3.885, P<0.01) and the tumorigenesis(430±36 vs. 297±21, t=5.492, P<0.01) in transfection group significantly reduced. The A value(0.803±0.101 vs. 1.050±0.140, t=2.855, P<0.05; 0.242±0.091 vs. 1.050±0.140, t=9.664, P<0.001) and the tumorigenesis(73±8 vs. 427±29, t=20.363, P<0.001; 305±22 vs .427±29, t=5.840, P<0.01) in experimental group 1 and experimental group 2 were significantly lower than that in the control group, while the A value(t=8.264, P<0.001) and the tumorigenesis(t=17.346, P<0.001) of experimental group 2 significantly decreased than that experimental group 1. Compared to the control group(0.967±0.094, 1.001±0.199, 1.000±0.101), the expressions of GOLPH3, P-gp and β-catenin in experimental group 1(3.634±0.574, 2.424±0.261, 2.324±0.418) significantly upregulated(t=7.944, P<0.01; t=7.520, P<0.01; t=5.330, P<0.01), while the expressions of these proteins in experimental group 2(0.520±0.176, 0.466±0.159, 0.933±0.049) were significantly lower than that in experimental group 1(t=8.983, P<0.01; t=11.106, P<0.001; t=5.724, P<0.01). \u0000 \u0000 \u0000Conclusion \u0000Expression of GOLPH3 is associated with resistance to 5-FU in colon cancer HT29 cell by activating Wnt/β-catenin signal pathway. \u0000 \u0000 \u0000Key words: \u0000Colonic neoplasms; Fluorouracil; Wnt signaling pathway; P-Glycoprotein","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43819110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-25DOI: 10.3760/J.ISSN:1007-631X.2001.08.012
Jie Yin, Chi Wang
Objective To observe the efficacy of arteriosclerosis embolization in the treatment of hepatic hemangio endothelioma (HHE)and the imaging manifestations of HHE. Methods Eight HHE children admitted to our hospital from Oct 2016 to Jan 2019 were enrolled to evaluate the efficacy of interventional therapy and the differentiation from other liver tumors. Results Eight patients underwent 10 interventional operations. The first embolization materials were gelatin sponge particles (2 000 μm). After the second embolization, microspheres (300-500 μm) were used for embolization materials. After superselective embolization, all 8 patients were weaned from the auxiliary ventilation. There was no hepatic venous development during the arterial phase after re-angiography. Tumor volume decreased or disappeared after chemoembolization. Tumors disappeared completely in 3 patients, shrank significantly in the remaining 5 cases. Conclusion Contrast-enhanced scanning is of great significance in the diagnosis of hepatic vascular endothelial cell tumor. Arteriosclerosis embolization has significant effect on children with hepatic vascular endothelial cell tumor. Key words: Liver neoplasms; Interventional therapy
{"title":"Imaging findings of hepatic hemangioendothelioma with arteriovenous shunt and evaluation of interventional therapy","authors":"Jie Yin, Chi Wang","doi":"10.3760/J.ISSN:1007-631X.2001.08.012","DOIUrl":"https://doi.org/10.3760/J.ISSN:1007-631X.2001.08.012","url":null,"abstract":"Objective \u0000To observe the efficacy of arteriosclerosis embolization in the treatment of hepatic hemangio endothelioma (HHE)and the imaging manifestations of HHE. \u0000 \u0000 \u0000Methods \u0000Eight HHE children admitted to our hospital from Oct 2016 to Jan 2019 were enrolled to evaluate the efficacy of interventional therapy and the differentiation from other liver tumors. \u0000 \u0000 \u0000Results \u0000Eight patients underwent 10 interventional operations. The first embolization materials were gelatin sponge particles (2 000 μm). After the second embolization, microspheres (300-500 μm) were used for embolization materials. After superselective embolization, all 8 patients were weaned from the auxiliary ventilation. There was no hepatic venous development during the arterial phase after re-angiography. Tumor volume decreased or disappeared after chemoembolization. Tumors disappeared completely in 3 patients, shrank significantly in the remaining 5 cases. \u0000 \u0000 \u0000Conclusion \u0000Contrast-enhanced scanning is of great significance in the diagnosis of hepatic vascular endothelial cell tumor. Arteriosclerosis embolization has significant effect on children with hepatic vascular endothelial cell tumor. \u0000 \u0000 \u0000Key words: \u0000Liver neoplasms; Interventional therapy","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47690305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-25DOI: 10.3760/CMA.J.ISSN.1007-631X.2019.08.008
Qi-zhi Liu, L. Hao, Z. Lou, L. Liu, Xian-Hua Gao, Yong-gang Hong
Objective To compare the characteristics of clinical pathology between patients with early recurrence and those with late recurrence of colorectal cancer. Methods Clinicopathological data of 391 recurrence patients after surgery from Changhai Hospital were recruited between Jan 2005 and Dec 2015. The clinical and pathological characteristics of primary cancer in early recurrence group (less than 2 years after surgery) and late recurrence group (2 year or more after surgery) were compared. Results 246 patients had early recurrence (62.9%) and 145 had late recurrence (37.1%). Liver, systemic metastases and peritoneum were the main sites of distant recurrence in the early recurrence group, whereas liver, lung and systemic metastases were the most frequent sites of metastases in the late recurrence group. Patients with the increased tumor perimeter, lymph node metastasis, increased CEA and CA19-9, without postoperative adjuvant treatment and microsatellite stability are more likely to have early recurrence. 5-year overall survival rate for patients with early recurrence was significantly lower than those with late recurrence. Conclusions This study showed that clinical and pathological factors are significantly associated with recurrence of colorectal cancer. Two years after surgery is an important period for the recurrence of colorectal cancer. Key words: Colorectal neoplasms; Recurrence; Neoplasm metastasis; Pathology, clinical
{"title":"Correlation of early and late recurrence with clinical and pathological factors in postoperative colorectal cancer patients","authors":"Qi-zhi Liu, L. Hao, Z. Lou, L. Liu, Xian-Hua Gao, Yong-gang Hong","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.08.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.08.008","url":null,"abstract":"Objective \u0000To compare the characteristics of clinical pathology between patients with early recurrence and those with late recurrence of colorectal cancer. \u0000 \u0000 \u0000Methods \u0000Clinicopathological data of 391 recurrence patients after surgery from Changhai Hospital were recruited between Jan 2005 and Dec 2015. The clinical and pathological characteristics of primary cancer in early recurrence group (less than 2 years after surgery) and late recurrence group (2 year or more after surgery) were compared. \u0000 \u0000 \u0000Results \u0000246 patients had early recurrence (62.9%) and 145 had late recurrence (37.1%). Liver, systemic metastases and peritoneum were the main sites of distant recurrence in the early recurrence group, whereas liver, lung and systemic metastases were the most frequent sites of metastases in the late recurrence group. Patients with the increased tumor perimeter, lymph node metastasis, increased CEA and CA19-9, without postoperative adjuvant treatment and microsatellite stability are more likely to have early recurrence. 5-year overall survival rate for patients with early recurrence was significantly lower than those with late recurrence. \u0000 \u0000 \u0000Conclusions \u0000This study showed that clinical and pathological factors are significantly associated with recurrence of colorectal cancer. Two years after surgery is an important period for the recurrence of colorectal cancer. \u0000 \u0000 \u0000Key words: \u0000Colorectal neoplasms; Recurrence; Neoplasm metastasis; Pathology, clinical","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47237773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-25DOI: 10.3760/CMA.J.ISSN.1007-631X.2019.08.005
Y. Diao, Shu-ping Tan, Y. Lan, Peng Li, Jiyang Wang, Guo-dong Ye, Da-jun Li
Objective To evaluate the efficacy of excimer laser atherectomy (ELA) combined with drug-coated balloon (DCB) in the treatment of lower limb arteriosclerosis obliterans. Methods From Sep 2016 to Dec 2018 42 patients (42 limbs) underwent ELA combined with DCB therapy. Results There were 27 males and 15 females.The mean age was (65.7±9.7) years. The average lesion length was (14.3±7.5) cm. The technical success rate was 100% and the remission rate of clinical symptoms was 92.9%. Ankle-brachial index was significantly improved from (0.45±0.15) pre-operation to (0.85±0.13) post-operation (P=0.001). The mean follow-up was 10.9 months. At 3, 6, and 12 months follow-up, the primary patency rate was 95.2%, 92.8% and 84.9%. The 1-year limb salvage rate was 95.2%. Conclusion ELA combined with DCB has a good 1-year results as our data reveal. Key words: Laser, excimer; Arteriosclerosis obliterans; Atherectomy; Drug-coated balloon; Angioplasty
{"title":"Excimer laser atherectomy combined with drug-eluting balloon for lower limb arteriosclerosis obliterans","authors":"Y. Diao, Shu-ping Tan, Y. Lan, Peng Li, Jiyang Wang, Guo-dong Ye, Da-jun Li","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.08.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.08.005","url":null,"abstract":"Objective \u0000To evaluate the efficacy of excimer laser atherectomy (ELA) combined with drug-coated balloon (DCB) in the treatment of lower limb arteriosclerosis obliterans. \u0000 \u0000 \u0000Methods \u0000From Sep 2016 to Dec 2018 42 patients (42 limbs) underwent ELA combined with DCB therapy. \u0000 \u0000 \u0000Results \u0000There were 27 males and 15 females.The mean age was (65.7±9.7) years. The average lesion length was (14.3±7.5) cm. The technical success rate was 100% and the remission rate of clinical symptoms was 92.9%. Ankle-brachial index was significantly improved from (0.45±0.15) pre-operation to (0.85±0.13) post-operation (P=0.001). The mean follow-up was 10.9 months. At 3, 6, and 12 months follow-up, the primary patency rate was 95.2%, 92.8% and 84.9%. The 1-year limb salvage rate was 95.2%. \u0000 \u0000 \u0000Conclusion \u0000ELA combined with DCB has a good 1-year results as our data reveal. \u0000 \u0000 \u0000Key words: \u0000Laser, excimer; Arteriosclerosis obliterans; Atherectomy; Drug-coated balloon; Angioplasty","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45842337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.3760/CMA.J.ISSN.1007-631X.2019.07.012
Jun-lai Zhao, Weiwei Wu, K. Zhao, Z. Cao, Yu Yang, C. Jiang
Objective To evaluate the experience and early results of radiofrequency ablation(RFA) therapy for varicose veins. Methods In this study 380 extremities of 356 patients undergoing RFA therapy with RFA catheter for varicose veins from Nov 2015 to Sep 2017 in Beijing Tsinghua Changgung Hospital were retrospectively reviewed. Each patient was scheduled to follow up at 1 , 6 and 12 months respectively. Results Technical success were achieved in all cases. Obliteration rate was 99.0% in 1 month, 97.9% in 6 months and 97.1% in 12 months after the RFA therapy. Conclusions RFA therapy for varicose veins is safe and effective and achieves good early result. Intraoperative ultrasound examination and endovascular technique is important for a successful RFA procedure. Key words: Varicose veins; Catheter ablation; Obliteration rate
{"title":"Experience and early results of radiofrequency ablation therapy for varicose veins","authors":"Jun-lai Zhao, Weiwei Wu, K. Zhao, Z. Cao, Yu Yang, C. Jiang","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.07.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.07.012","url":null,"abstract":"Objective \u0000To evaluate the experience and early results of radiofrequency ablation(RFA) therapy for varicose veins. \u0000 \u0000 \u0000Methods \u0000In this study 380 extremities of 356 patients undergoing RFA therapy with RFA catheter for varicose veins from Nov 2015 to Sep 2017 in Beijing Tsinghua Changgung Hospital were retrospectively reviewed. Each patient was scheduled to follow up at 1 , 6 and 12 months respectively. \u0000 \u0000 \u0000Results \u0000Technical success were achieved in all cases. Obliteration rate was 99.0% in 1 month, 97.9% in 6 months and 97.1% in 12 months after the RFA therapy. \u0000 \u0000 \u0000Conclusions \u0000RFA therapy for varicose veins is safe and effective and achieves good early result. Intraoperative ultrasound examination and endovascular technique is important for a successful RFA procedure. \u0000 \u0000 \u0000Key words: \u0000Varicose veins; Catheter ablation; Obliteration rate","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44689303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To evaluate the effects of laparoscopic splenectomy and esophagogastric devascularization(LSPD) vs. open procedure(OSPD) on stress and immune function in patients with portal hypertension due to cirrhosis. Methods From June 2015 to June 2017 a total of 66 patients underwent LSPD or OSPD procedures. Results Plasma cortisol concentration in the LSPD group was lower than that in the OSPD group (Fgroup=18.85, P=0.020). Cortisol concentration in the two groups increased firstly and then decreased with time extension (Ftime=532.08 , P=0.000). The level of CD3+ , CD4+ , CD4+ /CD8+ in LSPD group was higher than that in OSPD group, and the level of CD8+ was lower than that in OSPD group (Fgroup=3.55, 21.47, 154.84, 64.29, P 38.0 ℃, and >3 days) is OSPD group is higher than in the LSPD group(χ2=5.49, 6.68, P=0.019, 0.010). Conclusion LSPD effectively reduces postoperative stress, protects immune function , decreases postoperative pleural and abdominal effusion and fever. Key words: Hypertension, portal; Hydrocortisone; T-lymphocytes subsets; Splenectomy
{"title":"Effects of laparoscopic splenectomy and esophagogastric devascularization on stress and immune function in patients with cirrhotic portal hypertension","authors":"Kunfu Dai, Shuai Ma, Xiaopei Hao, Yuting He, Tao Zhou, Guangjin Tian, Yadong Dong","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.07.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.07.016","url":null,"abstract":"Objective \u0000To evaluate the effects of laparoscopic splenectomy and esophagogastric devascularization(LSPD) vs. open procedure(OSPD) on stress and immune function in patients with portal hypertension due to cirrhosis. \u0000 \u0000 \u0000Methods \u0000From June 2015 to June 2017 a total of 66 patients underwent LSPD or OSPD procedures. \u0000 \u0000 \u0000Results \u0000Plasma cortisol concentration in the LSPD group was lower than that in the OSPD group (Fgroup=18.85, P=0.020). Cortisol concentration in the two groups increased firstly and then decreased with time extension (Ftime=532.08 , P=0.000). The level of CD3+ , CD4+ , CD4+ /CD8+ in LSPD group was higher than that in OSPD group, and the level of CD8+ was lower than that in OSPD group (Fgroup=3.55, 21.47, 154.84, 64.29, P 38.0 ℃, and >3 days) is OSPD group is higher than in the LSPD group(χ2=5.49, 6.68, P=0.019, 0.010). \u0000 \u0000 \u0000Conclusion \u0000LSPD effectively reduces postoperative stress, protects immune function , decreases postoperative pleural and abdominal effusion and fever. \u0000 \u0000 \u0000Key words: \u0000Hypertension, portal; Hydrocortisone; T-lymphocytes subsets; Splenectomy","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47183980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.3760/CMA.J.ISSN.1007-631X.2019.07.003
Jun-lin Li, Shao-liang Han
Objective To investigate the surgical indication and surgical procedures for Crohn disease. Methods Clinical data of 47 cases with Crohn disease were retrospectively analyzed. Results The main clinical manifestations were abdominal pain ( 35 cases), diarrhea (16 cases), emaciation and fatigue( 12 cases), abdominal mass (9 cases), intestinal obstruction (31 cases), intestinal adhesion (18 cases), intestinal perforation( 8 cases), intestinal bleeding ( 11 cases) , internal fistula ( 4 cases), abdominal abscess( 4 cases). Preoperative enteroscopy was performed in 23 cases, and 7 cases were diagnosed as Crohn′s disease. Operative procedures included colectomy in 15 cases, small bowel resection and intestinal adhesion lysis in 29 cases, ileostomy in 3 cases. Postoperative complications occurred in 13 cases, including incision dehiscence in 2 cases, intestinal fistula in 5 cases, there were 2 cases of stress ulcer, pulmonary infection in 1 case and short bowel syndrome in 1 case, early postoperative inflammatory bowel obstruction in 2 cases and death in 1 case. 44 patients were followed-up, for an average of 6.8 years. Recurrence of Crohn′s disease was found in 11 cases and canceration in 3 cases. Conclusions Surgery is still the mainstay for Crohn′s disease, and close follow-up is important for disease recurrence and canceration. Key words: Crohn disease; Surgical procedures, operative; Complications
{"title":"Surgical treatment of Crohn disease","authors":"Jun-lin Li, Shao-liang Han","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.07.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.07.003","url":null,"abstract":"Objective \u0000To investigate the surgical indication and surgical procedures for Crohn disease. \u0000 \u0000 \u0000Methods \u0000Clinical data of 47 cases with Crohn disease were retrospectively analyzed. \u0000 \u0000 \u0000Results \u0000The main clinical manifestations were abdominal pain ( 35 cases), diarrhea (16 cases), emaciation and fatigue( 12 cases), abdominal mass (9 cases), intestinal obstruction (31 cases), intestinal adhesion (18 cases), intestinal perforation( 8 cases), intestinal bleeding ( 11 cases) , internal fistula ( 4 cases), abdominal abscess( 4 cases). Preoperative enteroscopy was performed in 23 cases, and 7 cases were diagnosed as Crohn′s disease. Operative procedures included colectomy in 15 cases, small bowel resection and intestinal adhesion lysis in 29 cases, ileostomy in 3 cases. Postoperative complications occurred in 13 cases, including incision dehiscence in 2 cases, intestinal fistula in 5 cases, there were 2 cases of stress ulcer, pulmonary infection in 1 case and short bowel syndrome in 1 case, early postoperative inflammatory bowel obstruction in 2 cases and death in 1 case. 44 patients were followed-up, for an average of 6.8 years. Recurrence of Crohn′s disease was found in 11 cases and canceration in 3 cases. \u0000 \u0000 \u0000Conclusions \u0000Surgery is still the mainstay for Crohn′s disease, and close follow-up is important for disease recurrence and canceration. \u0000 \u0000 \u0000Key words: \u0000Crohn disease; Surgical procedures, operative; Complications","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48872929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.3760/CMA.J.ISSN.1007-631X.2019.07.005
Jun-xi Gao, Hao Gu, Yating Wang, Yingxin Wang, Lei Yang, W. Han, Tao Song
Objective To explore the feasibility and safety of radiofrequency ablation for difficult access liver cancer under percutaneous local anesthesia combined with contrast-enhanced ultrasonography. Methods 45 patients(62 lesions) in the experimental group were treated by percutaneous, local anesthesia combined with contrast-enhanced ultrasound and in some cases with artificial ascites assisted by radiofrequency ablation vs control group of 40 patients (54 lesions) receiving radiofrequency ablation guided by CT or ultrasound through laparoscopy or open surgery. The complications, and postoperative residual and recurrence rates were compared between the two groups. Results Of the all patients, 4 cases suffered from severe complications. The pain scores and the blood loss were less significant in the experimental group. There was no significant difference in tumor residual rate between the two groups when evaluated on one month after the procedures, and in the recurrence rate after three and six months. Conclusion Radiofrequency ablation for difficult liver cancer by percutaneous local anesthesia combined with contrast-enhanced ultrasonography is less traumatic and less of complications compared to traditional method with a similar tumor residual rate and recurrence rate. Key words: Carcinoma, hepatocellular; Catheter ablation
{"title":"Radiofrequency ablation for liver cancer in difficult place under percutaneous local anesthesia combined with contrast enhanced ultrasonography","authors":"Jun-xi Gao, Hao Gu, Yating Wang, Yingxin Wang, Lei Yang, W. Han, Tao Song","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.07.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.07.005","url":null,"abstract":"Objective \u0000To explore the feasibility and safety of radiofrequency ablation for difficult access liver cancer under percutaneous local anesthesia combined with contrast-enhanced ultrasonography. \u0000 \u0000 \u0000Methods \u000045 patients(62 lesions) in the experimental group were treated by percutaneous, local anesthesia combined with contrast-enhanced ultrasound and in some cases with artificial ascites assisted by radiofrequency ablation vs control group of 40 patients (54 lesions) receiving radiofrequency ablation guided by CT or ultrasound through laparoscopy or open surgery. The complications, and postoperative residual and recurrence rates were compared between the two groups. \u0000 \u0000 \u0000Results \u0000Of the all patients, 4 cases suffered from severe complications. The pain scores and the blood loss were less significant in the experimental group. There was no significant difference in tumor residual rate between the two groups when evaluated on one month after the procedures, and in the recurrence rate after three and six months. \u0000 \u0000 \u0000Conclusion \u0000Radiofrequency ablation for difficult liver cancer by percutaneous local anesthesia combined with contrast-enhanced ultrasonography is less traumatic and less of complications compared to traditional method with a similar tumor residual rate and recurrence rate. \u0000 \u0000 \u0000Key words: \u0000Carcinoma, hepatocellular; Catheter ablation","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45906304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.3760/CMA.J.ISSN.1007-631X.2019.07.017
Rui-ting Liu, Y. Hou, Xiangtian Wu, Guo-rong Wang, Chang Liu, J. Bai, J. Qiu, Likun Yan, Xiaojun Li, Xiaoqiang Wang
Objective To investigate the effects of lentivirus-mediated RNA interference (RNAi) targeting DNA binding protein A (dbpA) on the proliferation and the biological behavior of colorectal cancer cell line SW620. Methods The experiment was divided into 3 groups: KD group (siRNA-dbpA, lentivirus interference group), CON group (non-specific sequence group) and NC group (blank control group). The lentiviral vector siRNA-dbpA was constructed and verified by PCR and DNA sequencing. SW620 cells were transfected with siRNA-dbpA plasmid, nontargeting siRNA plasmid, or empty plasmid. After 48 h the transfection, the cells were examined for dbpA expression using Western blot. After 72 hrs transfection, flow cytometry was used to detect the cell apoptosis and cell cycle changes. The cell growth inhibition rate was detected by MTT(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide) assay, and then clone formation was detected, and the ability of SW620 cells to form tumors in vivo after dbpA was silenced was studied in nude mice. Results PCR analysis and DNA sequencing demonstrated that the RNAi sequence targeting dbpA gene was successfully inserted into the lentiviral vector. siRNA-dbpA transfection resulted in reduced expression of dbpA in SW620 cells. After transfection, the apoptosis rate of siRNA-dbpA-transfected cells increased to 26.60% ± 0.38%, significantly higher than that in cells transfected with the nontargeting plasmid or the empty plasmid 12.54%±0.25% and 4.46%±0.19%, respectively(F=28.159, P<0.01). The growth inhibition test indicate that the OD value of the fifth day in siRNA-dbpA group was 0.194±0.037, significantly lower than that in the other two groups 0.814±0.043 and 1.625±0.061, respectively(F=23.214, P<0.01). The colony formation number is 37±3, 64±5and 175±10 respectively, siRNA-dbpA is significantly higher than that in the other two groups(F=40.254, P<0.01). After the completion of nude mouse transplantation tumor model, through the detection of tumor volume, KD group (group siRNA-dbpA) tumor volume after 14 d and CON and NC group had obvious difference(F=38.256, P<0.05), and after 21d is more significant difference in tumor size(F=40.241, P<0.01), can be clearly observed after 35 d KD group (group siRNA-dbpA) growing tumors had differences with the control group(F=30.257, P<0.05). Conclusion Lentivirus-mediated RNAi targeting dbpA can effectively suppress the expression of dbpA in colorectal tumor in nude mice, it is proved that dbpA silencing has a significant inhibitory effect on the growth of living tumor cells and decrease the proliferation of the colorectal cells. Key words: Colorectal neoplasms; DNA binding proteins; Apoptosis; Cell proliferation
{"title":"Effect of lentivirus vector-mediated RNA interference dbpA gene silencing on the biological behavior of colorectal cancer cells","authors":"Rui-ting Liu, Y. Hou, Xiangtian Wu, Guo-rong Wang, Chang Liu, J. Bai, J. Qiu, Likun Yan, Xiaojun Li, Xiaoqiang Wang","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.07.017","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.07.017","url":null,"abstract":"Objective \u0000To investigate the effects of lentivirus-mediated RNA interference (RNAi) targeting DNA binding protein A (dbpA) on the proliferation and the biological behavior of colorectal cancer cell line SW620. \u0000 \u0000 \u0000Methods \u0000The experiment was divided into 3 groups: KD group (siRNA-dbpA, lentivirus interference group), CON group (non-specific sequence group) and NC group (blank control group). The lentiviral vector siRNA-dbpA was constructed and verified by PCR and DNA sequencing. SW620 cells were transfected with siRNA-dbpA plasmid, nontargeting siRNA plasmid, or empty plasmid. After 48 h the transfection, the cells were examined for dbpA expression using Western blot. After 72 hrs transfection, flow cytometry was used to detect the cell apoptosis and cell cycle changes. The cell growth inhibition rate was detected by MTT(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide) assay, and then clone formation was detected, and the ability of SW620 cells to form tumors in vivo after dbpA was silenced was studied in nude mice. \u0000 \u0000 \u0000Results \u0000PCR analysis and DNA sequencing demonstrated that the RNAi sequence targeting dbpA gene was successfully inserted into the lentiviral vector. siRNA-dbpA transfection resulted in reduced expression of dbpA in SW620 cells. After transfection, the apoptosis rate of siRNA-dbpA-transfected cells increased to 26.60% ± 0.38%, significantly higher than that in cells transfected with the nontargeting plasmid or the empty plasmid 12.54%±0.25% and 4.46%±0.19%, respectively(F=28.159, P<0.01). The growth inhibition test indicate that the OD value of the fifth day in siRNA-dbpA group was 0.194±0.037, significantly lower than that in the other two groups 0.814±0.043 and 1.625±0.061, respectively(F=23.214, P<0.01). The colony formation number is 37±3, 64±5and 175±10 respectively, siRNA-dbpA is significantly higher than that in the other two groups(F=40.254, P<0.01). After the completion of nude mouse transplantation tumor model, through the detection of tumor volume, KD group (group siRNA-dbpA) tumor volume after 14 d and CON and NC group had obvious difference(F=38.256, P<0.05), and after 21d is more significant difference in tumor size(F=40.241, P<0.01), can be clearly observed after 35 d KD group (group siRNA-dbpA) growing tumors had differences with the control group(F=30.257, P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Lentivirus-mediated RNAi targeting dbpA can effectively suppress the expression of dbpA in colorectal tumor in nude mice, it is proved that dbpA silencing has a significant inhibitory effect on the growth of living tumor cells and decrease the proliferation of the colorectal cells. \u0000 \u0000 \u0000Key words: \u0000Colorectal neoplasms; DNA binding proteins; Apoptosis; Cell proliferation","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44982915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-25DOI: 10.3760/CMA.J.ISSN.1007-631X.2019.07.001
Y. Wu, M. Xie, Yunlong Cai, Tao Wu, Shan-jun Huang, Xin Wang
Objective To investigate the correlation between clinicopathological features and lymph node metastasis in early gastric cancer(EGC) and evaluate the influence of lymph node metastasis on the overall survival. Methods The clinicopathological data of 178 EGC patients undergoing radical gastrectomy with lymphadenectomy in Peking University First Hospital between Jan 2006 and Dec 2015 were retrospectively reviewed. The impact of lymph node metastasis on the overall survival was analyzed. Results Lymph node metastasis was detected in 19 (10.7%) of 178 patients. Univariate analysis showed a positive relationship between tumor size (χ2=4.804, P=0.028), depth of invasion(χ2=8.176, P=0.003), histological type (χ2=4.333, P=0.037), vascular tumor thrombus (χ2=9.992, P=0.002) and lymph node metastasis in EGC. Multivariate analysis revealed that depth of invasion(Wald=4.954, P=0.026) and intra-vascular tumor thrombus (Wald=3.966, P=0.046)were independent relative factors of lymph node metastasis in EGC patients. The 5-year survival rates of EGC patients with lymph node metastasis were 78.9%, much lower than that without lymph node metastasis (94.3%, χ2=8.310, P=0.004). Conclusions Lymph node metastasis in EGC is mainly correlated with depth of invasion and intra-vascular tumor thrombus. The prognosis of patients with lymph node metastasis is significantly poorer than those without lymph node metastasis. Key words: Stomach neoplasms; Lymphatic metastasis; Prognosis
{"title":"Lymph node metastasis and prognostic analysis of early gastric cancer patients","authors":"Y. Wu, M. Xie, Yunlong Cai, Tao Wu, Shan-jun Huang, Xin Wang","doi":"10.3760/CMA.J.ISSN.1007-631X.2019.07.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2019.07.001","url":null,"abstract":"Objective \u0000To investigate the correlation between clinicopathological features and lymph node metastasis in early gastric cancer(EGC) and evaluate the influence of lymph node metastasis on the overall survival. \u0000 \u0000 \u0000Methods \u0000The clinicopathological data of 178 EGC patients undergoing radical gastrectomy with lymphadenectomy in Peking University First Hospital between Jan 2006 and Dec 2015 were retrospectively reviewed. The impact of lymph node metastasis on the overall survival was analyzed. \u0000 \u0000 \u0000Results \u0000Lymph node metastasis was detected in 19 (10.7%) of 178 patients. Univariate analysis showed a positive relationship between tumor size (χ2=4.804, P=0.028), depth of invasion(χ2=8.176, P=0.003), histological type (χ2=4.333, P=0.037), vascular tumor thrombus (χ2=9.992, P=0.002) and lymph node metastasis in EGC. Multivariate analysis revealed that depth of invasion(Wald=4.954, P=0.026) and intra-vascular tumor thrombus (Wald=3.966, P=0.046)were independent relative factors of lymph node metastasis in EGC patients. The 5-year survival rates of EGC patients with lymph node metastasis were 78.9%, much lower than that without lymph node metastasis (94.3%, χ2=8.310, P=0.004). \u0000 \u0000 \u0000Conclusions \u0000Lymph node metastasis in EGC is mainly correlated with depth of invasion and intra-vascular tumor thrombus. The prognosis of patients with lymph node metastasis is significantly poorer than those without lymph node metastasis. \u0000 \u0000 \u0000Key words: \u0000Stomach neoplasms; Lymphatic metastasis; Prognosis","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43475736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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