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Effect of edible coating based on zedo gum containing Thymus essential oil and Cinnamomum extract on quality of cheddar cheese 含胸腺精油和肉桂提取物的莪术胶可食性涂层对切达奶酪品质的影响
4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-16 DOI: 10.1556/066.2023.00177
A.H.K. Aghdam, Sh. Zomorodi, L. Roufegarinejad, A. Khosrowshahi asl, Sh. Hanifian
Abstract Effect of edible coatings based on zedo gum (ZG) containing thyme ( Thymus vulgaris ) essential oil (TEO) and cinnamon ( Cinnamomum verum ) extract (CAE) on the quality and proteolysis of cheddar cheese was investigated. Four treatments were prepared using different coating formulas including: C (coated cheese with paraffin and wax mixture as commercial coating), Z treatment (coated cheese with ZG solution), TEO treatment (coated cheese with TEO), and CAE treatment (coated cheese with CAE). The results showed that the highest moisture content and proteolysis indices were related to sample C; the lowest moisture content and number of starter lactic acid bacteria (SLAB) and the highest protein content, FDM content, proteolysis indices and number of non-starter lactic acid bacteria (NSLAB) were related to sample Z; while the TEO and CAE treatments showed similar values. Also, TEO and CAE had an inhibitory effect on growth of moulds and yeasts, without affecting NSLAB and SLAB. Therefore, instead of commercial coating, zedo gum containing T. vulgaris essential oil or C. verum extract can be used as active coating on cheddar cheese.
摘要研究了含百里香(thyymus vulgaris)精油(TEO)和肉桂(Cinnamomum verum)提取物(CAE)的莪术胶(ZG)可食性涂层对切达奶酪品质和蛋白水解的影响。采用不同的包衣配方制备了4种处理:C(石蜡混合物包衣奶酪作为商品包衣)、Z (ZG溶液包衣奶酪)、TEO (TEO包衣奶酪)和CAE (CAE包衣奶酪)。结果表明:样品C的含水率和蛋白水解指标最高;发酵剂乳酸菌(SLAB)的最低水分含量和数量,蛋白质含量、FDM含量、蛋白水解指标和非发酵剂乳酸菌(NSLAB)的最高数量与样品Z有关;TEO和CAE处理值相近。TEO和CAE对霉菌和酵母的生长有抑制作用,对NSLAB和SLAB没有影响。因此,在切达奶酪上使用含有寻常草精油或羊角草提取物的zedo胶代替商业涂层。
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引用次数: 0
Effect of combined drying on the physical properties and bioactive components of ‘Jonathan’ apple (Malus domestica L.) 复合干燥对“乔纳森”苹果物理特性及生物活性成分的影响
4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-02 DOI: 10.1556/066.2023.00114
T. Antal
Abstract The main objectives of the present study were to investigate the physical attributes and chemical characteristics of dried apple cubes. The apple cubes were dried in vacuum and infrared dryers at 40 °C and freeze dryer individually and in combination. The physical properties – texture and rehydration – of vacuum drying-assisted freeze-dried apple dices were close to the freeze-dried products. The hardness of vacuum drying-assisted freeze-dried apples was better, by at least 15.8%, than those dried by infrared-freeze, but rehydration capacities were similar. The freeze-dried control samples achieved a lower water activity (0.145) in comparison with infrared, vacuum, and combined dried ones. The infrared-freeze-dried samples retained 3.6 and 11.6% more polyphenols and antioxidant capacity than samples prepared by the other methods. The ascorbic acid content of the freeze-dried samples was significantly higher by about 59% than that of the hybrid dried samples.
摘要本研究的主要目的是研究干苹果块的物理特性和化学特性。将苹果块分别在40°C的真空干燥机和红外干燥机以及冷冻干燥机中进行干燥。真空干燥辅助冻干苹果丁的物性、质地和复水性能与冻干产品接近。真空干燥辅助冷冻干燥的苹果硬度比红外冷冻干燥的苹果硬度至少提高15.8%,但复水能力相似。冻干对照样品的水分活度较红外、真空和混合干燥样品低(0.145)。红外冻干样品的多酚含量和抗氧化能力分别比其他方法高3.6%和11.6%。冻干样品的抗坏血酸含量显著高于杂交干燥样品约59%。
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引用次数: 0
Rheological properties and characterisation of some bioactive components in flours made of different coloured sweet potato (Ipomoea batatas L.) genotypes 不同颜色甘薯(Ipomoea batatas L.)基因型面粉流变特性及部分生物活性成分的表征
4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-02 DOI: 10.1556/066.2023.00130
B. Mihály-Langó, K. Ács, A. Berényi, K. Maróti Tóth, Zs. Táborosi Ábrahám, T. Gáll, E. Ács
Abstract The popularity of sweet potatoes in Central Europe has been increasing recently, mainly the high-quality, perfect, fresh tubers are in demand. However, out of class grade tubers could be marketed in dried, grounded form as sweet potato flour. The aim of this study was to characterise some important nutritional properties of flours of three sweet potato genotypes with different tuber colours (white, purple, and orange) and to investigate how this raw material affects the rheological properties of sweet potato-wheat flour blends. Dietary fibres are present in sweet potatoes in a significant proportion, orange coloured flour showed the highest values. The main free sugars were sucrose, glucose, and fructose, but sucrose was the dominant one. Antioxidant capacity and total phenolic content also varied considerably, the purple flour had the highest values. Mineral composition showed significant variability, the purple flour contained the highest level of minerals. It was confirmed that adding sweet potato flour to wheat flour affected its rheological properties, however in a varied manner. For the orange flour these properties have lightly decreased, though it had no significant effect on dough quality, while the white and purple flours with a dosage of 5, 10 and 15% could improve the dough behaviour. Thus, sweet potato in this form is a valuable raw material.
近年来,红薯在中欧的流行程度不断提高,主要是对优质、完美、新鲜的块茎的需求。然而,不合格的块茎可以以干燥、磨碎的形式作为红薯粉销售。本研究的目的是表征具有不同块茎颜色(白色、紫色和橙色)的三种甘薯基因型面粉的一些重要营养特性,并研究这种原料如何影响甘薯-小麦粉混合物的流变特性。红薯中含有大量膳食纤维,其中橙色面粉的含量最高。游离糖主要为蔗糖、葡萄糖和果糖,但蔗糖占主导地位。抗氧化能力和总酚含量也有较大差异,以紫色粉最高。矿物组成差异显著,紫粉中矿物质含量最高。经证实,在小麦粉中加入红薯粉对其流变特性有影响,但影响的方式各不相同。对于橙色面粉,这些性能略有下降,尽管它对面团质量没有显著影响,而白色和紫色面粉的用量分别为5%、10%和15%,可以改善面团的性能。因此,这种形式的甘薯是一种有价值的原料。
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引用次数: 0
Influence of the harvesting seasons on the polyphenol composition and antimicrobial activity of stinging nettle (Urtica dioica L.) extracts 采收季节对刺荨麻提取物多酚成分及抑菌活性的影响
4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-09-29 DOI: 10.1556/066.2023.00156
K. Kőszegi, Gy. Végvári, É. Stefanovits-Bányai, E. Békássy-Molnár, A. Maráz
Abstract The aim of our research was to study the water and ethanol extractable polyphenols of stinging nettle ( Urtica dioica L.) harvested in different seasons and to determine their antimicrobial activity against certain human pathogenic and food spoiling bacteria and yeasts. Our results indicate that the spring leaf extracts had higher polyphenol contents than the root one; however, close to the end of the vegetation period these values decreased considerably in both leaves and roots. Detection and quantification of the most abundant phenolic compounds in the spring extracts by HPLC revealed the occurrence of 12 different phenol carboxylic acids and flavonoids. Flavonoid compounds were more abundant than phenol carboxylic acids in the leaves; however, their proportion was equal in the case of the roots. Nettle leaf extracts had remarkable antimicrobial activity, the spring extracts were more efficient than the autumn ones. Escherichia coli and Staphylococcus aureus were sensitive to every leaf extract, while Listeria monocytogenes and Pseudomonas aeruginosa had reduced but remarkable sensitivity patterns. Among the yeasts, Candida glabrata was strongly inhibited by the aqueous leaf extracts. Most of the strains were insensitive to the root extracts, although Enterococcus faecalis was inhibited by the root and not the leaf extracts.
摘要本研究旨在研究不同季节采收的刺荨麻(Urtica dioica L.)水提物和乙醇提取物中的多酚类物质,并测定其对某些人类致病菌和食品腐败菌及酵母的抑菌活性。结果表明,春叶提取物的多酚含量高于根提取物;然而,在接近植被期结束时,这些值在叶和根中都显著下降。利用高效液相色谱法对春提取物中最丰富的酚类化合物进行检测和定量,发现存在12种不同的酚羧酸和黄酮类化合物。黄酮类化合物含量高于酚类羧酸;然而,在根的情况下,它们的比例是相等的。荨麻叶提取物具有显著的抑菌活性,春季提取物的抑菌活性高于秋季提取物。大肠杆菌和金黄色葡萄球菌对各叶提取物均有敏感性,单核增生李斯特菌和铜绿假单胞菌对各叶提取物的敏感性降低但显著。其中,叶片水提物对光念珠菌有较强的抑制作用。大多数菌株对根提取物不敏感,但粪肠球菌受到根而不是叶提取物的抑制。
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引用次数: 0
Spectrofluorimetric determination of chlorophyll a in arugula extracts 分光光度法测定芝麻菜提取物中叶绿素a的含量
4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-09-27 DOI: 10.1556/066.2023.00119
M. Čakić Semenčić, J. Smoljo, F. Šupljika
Abstract As a principal pigment in plants, chlorophyll a (Chl a) is widely used to evaluate quality changes and senescence process during storage of leafy vegetables. The determination of Chl a in plant extracts by spectrophotometric methods using various empirical equations is often unreliable. Considering the sensitivity of fluorescence detection, we report here a simple, inexpensive spectrofluorimetric method that can detect and quantify Chl a in plant extracts. The fluorescence standard used for the quantitative determination of Chl a was isolated in our laboratory from the extract of Anthrospira platensis (Spirulina). The method proved to be reliable, fast, and low cost in a study of the influence of the most commonly used domestic storage conditions on Chl a degradation in fresh-cut arugula.
叶绿素a (Chl a)作为植物的主要色素,被广泛用于评价叶菜在贮藏过程中的品质变化和衰老过程。采用各种经验方程的分光光度法测定植物提取物中的Chl - a往往不可靠。考虑到荧光检测的灵敏度,我们在这里报道了一种简单、廉价的荧光光谱法,可以检测和定量植物提取物中的Chl a。本实验室从platanthrospira spirensis (spirina)提取液中分离出用于测定Chl a的荧光标准品。通过对国内常用贮藏条件对鲜切芝麻菜中Chl - a降解影响的研究,证明该方法可靠、快速、成本低。
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引用次数: 0
Green synthesis of silver nanoparticles using Sorghum bicolor var. technicum seed extract, their characterisation and investigation of biological activities 高梁种子提取物绿色合成纳米银、表征及生物活性研究
4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-09-11 DOI: 10.1556/066.2023.00045
B. Dalgıç, N. Demir
Abstract Synthesis of nanoparticles can be long and costly processes using physical and chemical methods. Biological synthesis of nanoparticles is known to be cheaper and easier than other methods. In this study, silver nanoparticles (AgNP) were obtained by biological synthesis, also known as green synthesis, using Sorghum bicolor var. technicum (Körn) Stapf ex Holland seed extract, popularly known as sorghum. AgNPs were characterised by SEM, EDS, TEM, FT-IR, and UV-Vis Spectroscopy. SEM images confirmed that the shape of AgNPs was spherical. TEM analysis showed that the average sizes of AgNPs ranged from 51 to 56 nm. EDS analysis confirmed the presence of AgNPs by detecting a silver ion peak at 3 KeV. UV-Vis spectroscopy analyses indicated that the brown-burgundy colour of AgNPs exhibited maximum absorbance at 450 nm. The biological activities of the extract and AgNPs were investigated through antimicrobial, antibiofilm, antioxidant, mutagenic, and DNA cleavage activity analyses. The extract exhibited the highest MIC value against Gram-positive bacterium Bacillus subtilis (0.62 μg mL −1 ), whereas AgNPs demonstrated the highest antimicrobial activity specifically against Gram-negative bacterium Escherichia coli (0.31 μg mL −1 ). The antibiofilm results revealed that the extract displayed the highest percentage of biofilm inhibition against B. subtilis , while AgNPs exhibited notable efficacy against both Candida albicans yeast and Pseudomonas aeruginosa bacterium. The antioxidant activities were evaluated using DPPH • and ABTS •+ methods, and it was determined that both samples had high antioxidant activity. Mutagenicity of the extract and AgNPs were evaluated by the Ames/ Salmonella test using two strains of Salmonella typhimurium (TA98 and TA100). The mutagenic activity of the extract increased depending on the concentration for both strains, while AgNP did not show mutagenicity at any concentration. The agarose gel electrophoresis method showed that the extract and AgNPs cleaved DNA in the presence of an oxidising agent.
利用物理和化学方法合成纳米粒子是一个漫长而昂贵的过程。众所周知,生物合成纳米粒子比其他方法更便宜、更容易。在本研究中,利用高粱(Körn) Stapf ex Holland种子提取物,俗称高粱,通过生物合成获得了纳米银(AgNP),也称为绿色合成。用SEM、EDS、TEM、FT-IR和UV-Vis光谱对AgNPs进行了表征。SEM图像证实AgNPs的形状为球形。TEM分析表明,AgNPs的平均尺寸在51 ~ 56 nm之间。能谱分析通过检测到3 KeV的银离子峰证实了AgNPs的存在。紫外可见光谱分析表明,AgNPs在450 nm处的吸光度最大,呈棕紫红色。通过抗菌、抗生物膜、抗氧化、诱变和DNA裂解活性分析,对提取物和AgNPs的生物活性进行了研究。该提取物对革兰氏阳性杆菌枯草芽孢杆菌的MIC值最高(0.62 μ mL−1),而AgNPs对革兰氏阴性杆菌大肠杆菌的MIC值最高(0.31 μ mL−1)。结果表明,AgNPs对枯草芽孢杆菌的生物膜抑制率最高,对白色念珠菌和铜绿假单胞菌的生物膜抑制率最高。采用DPPH•和ABTS•+方法对其抗氧化活性进行评价,结果表明,两种样品均具有较高的抗氧化活性。采用2株鼠伤寒沙门菌(TA98和TA100)进行Ames/ Salmonella试验,评价提取物和AgNPs的致突变性。提取物对两种菌株的诱变活性随浓度的增加而增加,而AgNP在任何浓度下都没有表现出诱变活性。琼脂糖凝胶电泳方法表明,提取物和AgNPs在氧化剂的存在下可切割DNA。
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引用次数: 0
Cloning, expression, and biochemical characterisation of a novel endomannanase from Thermobifida alba 一种新型白僵菌内甘露聚糖酶的克隆、表达和生化特性研究
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-09-04 DOI: 10.1556/066.2023.00186
Sz. Luzics, Á. Tóth, T. Barna, E. Szabó, I. Nagy, B. Horváth, I. Nagy, Z. Varecza, I. Bata-Vidács, J. Kukolya
Thermobifida alba is the mesophilic member of the Thermobifida genus, the genome and enzyme sets of which have not been described and published yet. Thermobifida strains are thermotolerant actinomycete, which possess wide sets of cellulose and hemicellulose hydrolysing enzymes. Previously, three endomannanases (Man5ATh, Man5ATc, and Man5AThf) of thermobifidas were cloned and investigated, and hereby the endomannanase of T. alba DSM 43795 is described. All four endomannanases belong to the glycoside hydrolase family 5, their sizes are around 50–55 kDa. Their structure consists of a catalytic domain and a carbohydrate binding module, while there is an interdomain linker region in-between consisting repetitive tetrapeptide motifs (eg.: PPTEPTD-Ta, PTDP-Tc, TEEP-Tf, DPGT-Th). The pH optima of Man5A enzymes from T. alba, Thermobifida halotolerans, Thermobifida cellulosilytica, and Thermobifida fusca are slightly different (6.5, 7.0, 7.5, and 8.0, respectively), however, the temperature optima of the enzymes were detected within a wider range of 65–75 °C. In this research, Man5ATa exhibited the lowest Michaelis-Menten constant (KM) (0.13 mM) on LBG-mannan substrate, while others shared similar kinetic parameters: 0.9–1.7 mM of KM. Despite the high sequence similarity of the investigated mannanases, they exhibit different temperature stability parameters. These different functional characteristics can be advantageous for industrial applications producing biologically active, oligomannan prebiotics under different conditions.
白热刺蝇是热刺蝇属的中温成员,其基因组和酶组尚未被描述和发表。嗜热菌株是耐热放线菌,具有广泛的纤维素和半纤维素水解酶。此前对热裂菌的三种内糖酶(Man5ATh、Man5ATc和Man5AThf)进行了克隆和研究,现对T. alba DSM 43795的内糖酶进行描述。这四种内生甘露酶都属于糖苷水解酶家族5,它们的大小在50-55 kDa左右。它们的结构由一个催化结构域和一个碳水化合物结合模块组成,而两者之间有一个由重复四肽基序组成的结构域间连接区域(例如。: PPTEPTD-Ta, PTDP-Tc, TEEP-Tf, DPGT-Th)。白僵菌(T. alba)、耐盐热菌(Thermobifida halotolerans)、纤维素热菌(Thermobifida cellulosilytica)和fusca热菌(Thermobifida fusca)的Man5A酶的最适pH值略有不同(分别为6.5、7.0、7.5和8.0),但酶的最适温度范围在65 ~ 75℃。在本研究中,Man5ATa在llb -mannan衬底上的Michaelis-Menten常数(KM)最低,为0.13 mM,而其他材料的动力学参数相似,为0.9 ~ 1.7 mM。尽管所研究的甘露聚糖酶具有较高的序列相似性,但它们具有不同的温度稳定性参数。这些不同的功能特征有利于在不同条件下生产具有生物活性的低甘露聚糖益生元的工业应用。
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引用次数: 0
Suitability of pearl millet (Pennisetum glaucum (L.) R. Br.) and sorghum (Sorghum bicolor (L.) Moench) based food products for diabetics 珍珠小米(Pennisetum glaucum(L.)R.Br.)和高粱(sorghum bicolor(L.)Moench)食品对糖尿病患者的适用性
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-08-16 DOI: 10.1556/066.2022.00144
A. Sewak, N. Singla, M. Javed, G. S. Gill, Acta Alimentaria
In the study, suitability of porridge, bun, and salad prepared from processed pearl millet FBC16 and sorghum PSC4 had been evaluated organoleptically by a panel of semi-trained judges and 25 non-insulin dependent diabetes mellitus subjects. Organoleptically, germinated pearl millet was found to be more suitable for porridge (50%) and salad (100%), while puffed sorghum was best suitable for bun (15%) preparation. Prepared porridge had significantly (P ≤ 0.05) higher protein (16.9%) and total phenols (178.8 mg GAE/100 g) contents and antioxidant capacity (1,036 mg TE/100 g) than control. The dietary fibre and in vitro starch digestibility of composite porridge and bun increased significantly (P ≤ 0.05). Most acceptable composition of porridge, bun, and salad had low glycaemic index (17.64–26.79) and medium to low glycaemic load (8.82–13.40). Suitability of pearl millet and sorghum using appropriate processing techniques (germination and puffing) is recommended for preparation of indigenous food products especially for diabetics.
在这项研究中,由半培训的法官和25名非胰岛素依赖型糖尿病受试者组成的小组从感官上评估了由加工珍珠小米FBC16和高粱PSC4制成的粥、面包和沙拉的适宜性。从感官上看,发芽的珍珠小米更适合做粥(50%)和沙拉(100%),而膨化高粱最适合做包子(15%)。调制粥的蛋白质含量(16.9%)和总酚含量(178.8)显著高于(P≤0.05) mg GAE/100 g) 含量和抗氧化能力(1036 毫克 TE/100克)比对照组大。复合粥和面包的膳食纤维和淀粉体外消化率显著提高(P≤0.05),和沙拉具有低血糖指数(17.64–26.79)和中低血糖负荷(8.82–13.40)。建议使用适当的加工技术(发芽和膨化)制备珍珠小米和高粱,以制备本土食品,尤其是糖尿病患者的本土食品。
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引用次数: 0
Variation in physio-chemical attributes and WUE during growth and development of Pak choi (Brassica rapa L. subsp. chinensis L.) under different drip fertigation and mulching treatments 白菜生长发育过程中理化特性和水分利用效率的变化。不同滴灌施肥和覆盖处理下的羊草(chinensis L.
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-08-14 DOI: 10.1556/066.2023.00084
P. Singla, R. Sharda, Suraj P. Sharma, D. Gulati, K. Pandey, S. Navprem, P.K. Singh, A. Sharma
In the modern era, nutraceutical properties of horticultural crops are indispensable to determine their adaptability to different agro-ecological regions. The present study exploits the potential of mulches (P: plastic mulch; S: straw mulch; N: No mulch) in relation to drip irrigation (I1: 100%, I2: 80%, I3: 60% of crop evapotranspiration (Etc)), and fertigation (F1: 100%, F2: 80%, F3: 60% of recommended dose of fertilizer (RDF): 125 kg N, 62.5 Kg P2O5, 62.5 kg K2O per ha) on Pak choi at three maturity stages in the North West region of India. Plant fresh weight was the highest at 55 days after transplanting (DAT); however, maximum soil plant analysis development (SPAD) values were registered at 45 DAT. Antioxidant activity, FRAP, DPPH, phenols, flavanols, total sugars, ascorbic acid, free amino acids, and irrigation water use efficiency were observed significantly higher in plants grown on plastic mulch at different levels of irrigation and fertigation. It is envisioned that Pak choi plants had the highest nutritional value at 45 DAT from plots mulched with silver-black, irrigated at 80% Etc, and fertigated at 100% RDF. The nutrient enriched plants are used for green salad and as ingredients for the preparation of many recipes in the semi-arid and sub-tropic areas of India.
在现代,园艺作物的营养特性是决定其对不同农业生态区域适应性的不可或缺的因素。本研究利用了覆盖物(P:塑料覆盖物;S:秸秆覆盖物;N:无覆盖物)与滴灌(I1:100%,I2:80%,I3:60%作物蒸散量(Etc))和灌溉施肥(F1:100%,F2:80%,F3:60%推荐施肥量(RDF))的关系:125 公斤 N、 62.5 公斤 P2O5,62.5 公斤 K2O/ha)在印度西北地区小白菜的三个成熟阶段。移植后55天植株鲜重最高(DAT);然而,最大土壤植物分析发展(SPAD)值记录在45DAT。在不同灌溉和施肥水平下,地膜覆盖植物的抗氧化活性、FRAP、DPPH、酚类、黄烷醇、总糖、抗坏血酸、游离氨基酸和灌溉用水效率显著较高。可以设想,在用银黑色覆盖、以80%Etc灌溉和以100%RDF施肥的地块中,小白菜植物在45DAT时具有最高的营养价值。在印度的半干旱和亚热带地区,富含营养的植物被用于制作绿色沙拉,并被用作许多食谱的配料。
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引用次数: 0
Enzymatic hydrolysates from camel colostrum and milk proteins display inhibitory activity toward a key enzyme related to obesity 骆驼初乳和乳蛋白的酶解物对一种与肥胖相关的关键酶显示出抑制活性
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-08-14 DOI: 10.1556/066.2023.00091
O. Olfa, J. Zeineb, E. Halima, Acta Alimentaria
This study aimed to investigate the anti-obesity potential of camel colostrum and milk proteins as well as their enzymatic hydrolysates. Camel colostrum and milk proteins were treated using six proteolytic enzymes (pepsin, trypsin, α-chymotrypsin, pancreatin, papain, and pronase). The degree of hydrolysis was measured to verify the degradation of proteins. The in vitro anti-obesity activity was evaluated using the pancreatic lipase inhibitory assay. Camel colostrum and milk protein hydrolysates exhibited different degrees of hydrolysis ranging from 17.69 to 43.97%. The protein content varied between 56.08–61.95% and 37.39–41.72% for camel colostrum and milk protein hydrolysates, respectively. The hydrolysates displayed significantly higher anti-obesity activity than the undigested proteins at all tested concentrations (P < 0.05). Colostrum protein hydrolysate generated with pancreatin had the highest anti-obesity potential (59.92%). These results suggest that colostrum and milk protein hydrolysates could be used to formulate functional foods and nutraceuticals.
本研究旨在研究骆驼初乳和乳蛋白及其酶解产物的抗肥胖潜力。用六种蛋白水解酶(胃蛋白酶、胰蛋白酶、α-糜蛋白酶、胰酶、木瓜蛋白酶和蛋白酶)处理骆驼初乳和乳蛋白。测量水解程度以验证蛋白质的降解。使用胰脂肪酶抑制试验评价体外抗肥胖活性。骆驼初乳和乳蛋白水解物的水解度不同,从17.69到43.97%不等。骆驼初乳和奶蛋白水解物中的蛋白质含量分别在56.08到61.95%和37.39到41.72%之间。在所有测试浓度下,水解产物均显示出显著高于未消化蛋白质的抗肥胖活性(P<0.05)。胰蛋白酶产生的牛初乳蛋白水解产物具有最高的抗肥胖潜力(59.92%)。这些结果表明,初乳和乳蛋白水解物可用于配制功能性食品和营养品。
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Acta Alimentaria
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