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Visualisation of Phyllanthus emblica L. quality using E-nose, HS-SPME/GC-MS, and spectrophotometric methods with chemometrics 利用电子鼻、HS-SPME/GC-MS和化学计量学分光光度法对余甘子质量的可视化
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-08-09 DOI: 10.1556/066.2023.00068
X. Liu, Y. Li, J. Chen, Daqing He, H. Li, K. Wu
This paper provides a rapid method coupled with chemometrics to visualise PEL quality of eight regions. The contents of mineral elements, Vitamin C (Vc), and colour parameters were measured using spectrophotometric methods. The volatile substances were determined by electronic nose (E-nose) and headspace solid phase micro-extraction with gas chromatography-mass spectrometry (HS-SPME/GC-MS). Chemometric analyses were employed to visualise the sample distribution according to the geographical origin. The colour parameters, Vc, and mineral contents of PEL from diverse origins were significantly different (P < 0.05). A total of 25 volatile organic compounds (VOCs) were identified from PELs, with the highest percentage of components being ethyl acetate. The combination of HS-SPME/GC-MS and E-nose can properly characterise PEL samples. Therefore, the results of this exploratory work highlight the possibility of discriminating PEL from different regions.
本文提供了一种结合化学计量学的快速方法来可视化八个区域的PEL质量。采用分光光度法测定了矿物元素、维生素C(Vc)的含量和颜色参数。采用电子鼻(E-nose)和气相色谱-质谱(HS-SPME/GC-MS)顶空固相微萃取法测定挥发性物质。化学计量分析用于根据地理来源可视化样本分布。不同产地PEL的颜色参数、Vc和矿物含量差异显著(P<0.05)。从PEL中共鉴定出25种挥发性有机化合物,其中乙酸乙酯含量最高。HS-SPME/GC-MS和E-nose的组合可以正确地表征PEL样品。因此,这项探索性工作的结果突出了区分不同地区PEL的可能性。
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引用次数: 0
Nondestructive detection of mango soluble solid content in hyperspectral imaging based on multi-combinatorial feature wavelength selection 基于多组合特征波长选择的芒果可溶性固形物高光谱成像无损检测
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-08-09 DOI: 10.1556/066.2023.00014
J.J. Lin, Q.H. Meng, Z.F. Wu, S.Y. Pei, P. Tian, X. Huang, Z. Qiu, H.J. Chang, C. Ni, Y.Q. Huang, Y. Li
This paper explores the prediction of the soluble solid content (SSC) in the visible and near-infrared (400–1,000 nm) regions of Baise mango. Hyperspectral images of Baise mangoes with wavelengths of 400–1,000 nm were obtained using a hyperspectral imaging system. Multiple scatter correction (MSC) was chosen to remove the effect of noise on the accuracy of the partial least squares (PLS) regression model. On this basis, the characteristic wavelengths of mango SSC were selected using the competitive adaptive reweighted sampling (CARS), genetic algorithm (GA), uninformative variable elimination (UVE), and combined CARS + GA-SPA, CARS + UVE-SPA, and GA + UVE-SPA characteristic wavelength methods. The results show that the combined MSC-CARS + GA-SPA-PLS algorithm can reduce redundant information and improve the computational efficiency, so it is an effective method to predict the SSC of mangoes.
探讨了百色芒果在可见光和近红外(400 - 1000 nm)区域的可溶性固形物含量(SSC)的预测方法。利用高光谱成像系统,获得了波长400 ~ 1000 nm的百色芒果高光谱图像。为了消除噪声对偏最小二乘(PLS)回归模型精度的影响,采用了多重散射校正(MSC)。在此基础上,采用竞争自适应重加权采样(CARS)、遗传算法(GA)、无信息变量消去(UVE)以及CARS + GA- spa、CARS + UVE- spa、GA + UVE- spa三种特征波长方法选择芒果SSC的特征波长。结果表明,MSC-CARS + GA-SPA-PLS组合算法可以减少冗余信息,提高计算效率,是预测芒果SSC的有效方法。
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引用次数: 0
Optimisation preparation, physicochemical properties, and glucose transport regulation by polysaccharides from Liubao brick tea 六堡砖茶多糖的优化制备、理化性质及葡萄糖转运调控
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-08-07 DOI: 10.1556/066.2023.00115
H.Q. Wang, Y.Z. Sun, B.B. Li, W. Zhang
In this study, a water-soluble novel polysaccharide called TPS was successfully prepared and isolated from Liubao tea. The optimal extraction conditions resulted in a yield of 10.70% for the crude TPS. The purified TPS exhibited unique physicochemical properties and structural characteristics. It was identified as an acidic polysaccharide with trace binding proteins, with a →4)-α-D-Galp-(1→) residue. The purified TPS had a dense and uneven appearance, potential crystallisation characteristics, and structural stability. Importantly, it demonstrated the ability to inhibit glucose transport in Caco-2 cells by down-regulating the expression of sodium/glucose cotransporter 1 (SGLT1) and glucose transporter 2 (GLUT2), leading to a hypoglycemic effect. These findings highlight the potential of TPS from Liubao tea as a functional food or additive with hypoglycaemic properties.
本研究从六宝茶中成功制备并分离出一种水溶性新型多糖TPS。在最佳提取条件下,粗TPS的收率为10.70%。纯化后的TPS具有独特的理化性质和结构特征。它被鉴定为一种酸性多糖,具有微量结合蛋白→4) -α-D-Galp-(1→) 残留纯化的TPS具有致密和不均匀的外观、潜在的结晶特性和结构稳定性。重要的是,它证明了通过下调钠/葡萄糖协同转运蛋白1(SGLT1)和葡萄糖转运蛋白2(GLUT2)的表达来抑制Caco-2细胞中葡萄糖转运的能力,从而产生降血糖作用。这些发现突出了六宝茶TPS作为具有低血糖特性的功能性食品或添加剂的潜力。
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引用次数: 1
Assessment of bacteriocin producing Enterococcus faecium HZ as adjunct culture to improve the aroma formation and antimicrobial activity in white-brined cheese 产生细菌素的粪肠球菌HZ作为辅助培养物改善白卤奶酪香气形成和抗菌活性的评价
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-08-02 DOI: 10.1556/066.2023.00086
S. Ozturkoglu-Budak, M. Arkadaş, Z. Yıldırım, Y. K. Avşar
Cultures used in dairy products make it possible to obtain standard industrial products. However, they all provide a uniform taste and aroma. Generally, non-starter lactic acid bacteria (NSLAB) isolated from raw-milk or artisanal cheeses offer varied sensory characteristics when integrated in cheese provided that biosafety criteria are met. Enterococcus faecium HZ was previously isolated from traditional Turkish cheese and determined to have strong antibacterial activity as well as no gelatinase and hemolysis activities. In this study, this strain was used as adjunct culture in white-brined cheese to improve the physicochemical, textural, and aromatic properties, as well as antimicrobial activity. Cheeses with E. faecium HZ had a higher sensory score, which could be due to the aroma-active compounds produced by this strain. The incorporation of E. faecium HZ also improved the microbial quality of cheeses and showed an inhibitory effect via a stable enterocin production on indicator microorganisms.
乳制品中使用的培养物使获得标准的工业产品成为可能。然而,它们都提供统一的味道和香气。一般来说,从生牛奶或手工奶酪中分离出的非发酵剂乳酸菌(NSLAB)在满足生物安全标准的情况下,与奶酪结合后具有不同的感官特性。粪肠球菌HZ先前从传统的土耳其奶酪中分离出来,并被确定具有很强的抗菌活性,但没有明胶酶和溶血活性。在本研究中,将该菌株作为辅助培养物用于白盐水奶酪中,以改善其理化、质地、芳香特性和抗菌活性。含有粪肠杆菌HZ的奶酪具有更高的感官评分,这可能是由于该菌株产生的芳香活性化合物。E. faecium HZ的掺入也提高了奶酪的微生物品质,并通过稳定的肠球菌素产量对指示微生物表现出抑制作用。
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引用次数: 0
Total phenolic, flavonoid, protein contents and biological activities of wild mustard 野生芥菜总酚、类黄酮、蛋白质含量及生物活性
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-07-24 DOI: 10.1556/066.2023.00082
F. Mohammed, M. Sevindik, I. Uysal
The nutritional and therapeutic benefits of plants are outstanding. Wild mustard (Sinapis arvensis L.) collected from different regions was analysed for its phenolic, flavonoid, protein, and biological activity levels. In this case, the plant ethanol extract was obtained using a soxhlet apparatus. Rel assay kits and the DPPH test were used to assess the plant's antioxidant activity. The agar dilution test was used to determine antimicrobial efficacy. A549 lung cancer cells were used in an MTT assay to measure antiproliferative activity. The Folin−Ciocalteu reagent was used to quantify the total phenolic content of the sample. The amount of flavonoids was determined using an aluminium chloride test. The amount of protein was calculated using the AOAC's standard technique. Based on the research conducted, it was shown that the maximum total antioxidant status (TAS) value for the ethanol extract of wild mustard from various places was 5.232 ± 0.047 mmol L−1. At a concentration of 2 mg mL−1, DPPH activity was measured to be 82.06 ± 1.01. The maximum levels of total phenolic, flavonoid, and protein were 80.57 ± 2.19 mg g−1, 154.07 ± 2.79 mg g−1, and 7.75 ± 0.24%, respectively. Doses of 25–100 μg mL−1 of plant extracts were effective against fungal strains, whereas doses of 50–200 μg mL−1 were beneficial against bacterial strains. The plant extracts were shown to have potent antiproliferative properties. It was found that wild mustard's phenolic, flavonoid, protein, and biological activity levels varied according to the location from which it was gathered. It was also concluded that wild mustard had significant biological activity.
植物的营养和治疗效益是突出的。对不同产地的野生芥菜(Sinapis arvensis L.)的酚类、类黄酮、蛋白质和生物活性进行了分析。在这种情况下,使用索氏装置获得植物乙醇提取物。采用Rel测定试剂盒和DPPH法测定植株的抗氧化活性。采用琼脂稀释法测定其抑菌效果。用MTT法测定A549肺癌细胞的抗增殖活性。采用Folin - Ciocalteu试剂定量测定样品中总酚含量。采用氯化铝法测定黄酮类化合物的含量。用AOAC的标准技术计算蛋白质的量。研究表明,各地野芥菜乙醇提取物的最大总抗氧化状态(TAS)值为5.232±0.047 mmol L−1。浓度为2 mg mL−1时,DPPH活性为82.06±1.01。总酚、类黄酮和蛋白质的最高含量分别为80.57±2.19 mg g−1、154.07±2.79 mg g−1和7.75±0.24%。25 ~ 100 μ mL−1的剂量对真菌菌株有效,而50 ~ 200 μ mL−1的剂量对细菌菌株有益。植物提取物被证明具有有效的抗增殖特性。发现野生芥菜的酚类、类黄酮、蛋白质和生物活性水平随采集地点的不同而变化。结果表明,野生芥菜具有显著的生物活性。
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引用次数: 0
Nutritional characterisation of homemade beef sausage based on amino acid, biogenic amines, and fatty acid composition 基于氨基酸、生物胺和脂肪酸组成的自制牛肉香肠的营养特性
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-07-20 DOI: 10.1556/066.2023.00071
K. Berisha, A. Gashi, Z. Mednyánszky, H. Bytyqi, L. Simon Sarkadi
The aim of this study was to assess the nutritional quality of homemade beef sausages by examining the amino acid, biogenic amine, and fatty acid composition. The most abundant amino acids were Ala (15.56%), Leu (13.28%), Gly (8.64%), Pro (8.41%), Ser (8.26%), and Val (7.65%). The essential amino acids accounted for 44.30% of total amino acids. Apart from the protein building amino acids, the free amino acid content was relatively high, accounting for 10% of total amino acid content. The average biogenic amine concentration in the sausage samples was low (1.69 mg kg−1). Saturated fatty acids accounted for 59.10% of total fatty acids, followed by monounsaturated (38.63%) and polyunsaturated fatty acids (2.27%). The fatty acid profile was dominated by oleic (C18:1, 34.37%) and palmitic (C16:0, 30.24%) acids, and short-chain fatty acids were also present, which may have a positive impact on gut health. The results show that beef sausages have a high nutritional value and are a good source of essential amino acids, free amino acids, and fatty acids that are important for human health.
本研究的目的是通过检测氨基酸、生物胺和脂肪酸组成来评估自制牛肉香肠的营养质量。氨基酸含量最高的是Ala(15.56%)、Leu(13.28%)、Gly(8.64%)、Pro(8.41%)、Ser(8.26%)和Val(7.65%)。必需氨基酸占总氨基酸的44.30%。除蛋白质构建氨基酸外,游离氨基酸含量较高,占总氨基酸含量的10%。香肠样品中生物胺的平均浓度较低(1.69 mg kg−1)。饱和脂肪酸占总脂肪酸的59.10%,其次是单不饱和脂肪酸(38.63%)和多不饱和脂肪酸(2.27%)。脂肪酸谱以油酸(C18:1, 34.37%)和棕榈酸(C16:0, 30.24%)为主,还存在短链脂肪酸,这可能对肠道健康有积极影响。结果表明,牛肉香肠营养价值高,是人体必需氨基酸、游离氨基酸和脂肪酸的良好来源,对人体健康很重要。
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引用次数: 0
Investigation of the effects of some wall materials and encapsulation techniques on the microencapsulation of olive leaf extract 不同壁材及包封工艺对橄榄叶提取物微胶囊化的影响
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-07-06 DOI: 10.1556/066.2022.00253
S. Kahraman, H. Demircan, K. Sarioglu, S. Demircan, R. A. Oral
In this study, it is aimed to encapsulate some functional components of the olive leaves. Olive leaf extract was encapsulated using solution of sodium alginate, sodium alginate/gelatine, and sodium alginate/agar as wall material by ionic gelation technique. Also, olive leaf extract was encapsulated using solution of gelatine as wall material by cold gelation technique. The viscosities of the coating materials used in the study were investigated. An optimisation process was carried out to determine the injection time to be applied in the ionic gelation technique and the encapsulation efficiencies, particle sizes, swelling ratios, in vitro release profiles, and antioxidant activities of the obtained capsules were determined. While the encapsulation efficiency of the capsules obtained by the cold gelation technique was determined as the highest (98.2 ± 0.99%), it was revealed that the viscosity of the wall material used in the ionic gelation technique was important in the encapsulation efficiency. The particle size and swelling rate of the capsules obtained using the cold gelation technique were the highest. The release rate of oleuropein was generally higher at gastric pH than at intestinal pH. A correlation was found between antioxidant activities and the encapsulation efficiency of capsules.
在本研究中,它旨在封装橄榄叶的一些功能成分。以海藻酸钠、海藻酸/明胶和海藻酸盐/琼脂溶液为壁材,采用离子凝胶技术对橄榄叶提取物进行了包封。以明胶溶液为壁材,采用冷凝胶技术对橄榄叶提取物进行包封。对研究中使用的涂层材料的粘度进行了研究。进行优化过程以确定离子凝胶化技术中应用的注射时间,并确定所获得胶囊的包封效率、粒径、溶胀率、体外释放特性和抗氧化活性。虽然通过冷凝胶化技术获得的胶囊的包封效率最高(98.2±0.99%),但表明离子凝胶化技术中使用的壁材料的粘度对包封效率很重要。使用冷凝胶技术获得的胶囊的粒径和溶胀率最高。油尿蛋白在胃pH值下的释放速率通常高于在肠pH值下。抗氧化活性与胶囊的包封效率之间存在相关性。
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引用次数: 0
Optimisation of ultrasound-assisted extraction of carotenoids and antioxidant activity from Citrus reticulata Blanco peels (Wilking mandarin) 超声辅助提取柑橘皮类胡萝卜素及抗氧化活性的优化
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-07-03 DOI: 10.1556/066.2022.00222
A. Kadi, H. Boudries, M. Bachir-bey, M. Teffane, A. Taibi, Y. Arroul, L. Boulekbache‐Makhlouf
A large amount of waste, especially the outer part of citrus fruits (peel), is generated after consuming the pulp and it remains unused. The valorisation of this waste by recovering its bioactive compounds seems interesting. The aim of this study was to find the optimal conditions using ultrasound-assisted extraction (UAE) that yield the highest carotenoid content and better antioxidant activity from Citrus reticulata Blanco peels.Response surface methodology (RSM) through Box–Behnken experimental design was used to optimise the conditions for carotenoid extraction using UAE. Hexane concentration, temperature, and sonication time were selected as the main factors.The results revealed that all independent variables affected the responses. The optimal UAE conditions for hexane concentration, temperature, and sonication time were 60.76%, 36.45 °C, and 37.32 min, respectively. The values of total carotenoid content (TCC) and total antioxidant activity (TAA) obtained by UAE were higher than those obtained by the maceration extraction method.It can be concluded that the medium and extraction parameters, including hexane concentration, temperature, and sonication time, significantly influenced the recovery of carotenoids and antioxidant activity. The optimisation study allowed determining the appropriate conditions to maximise both responses. Compared to conventional maceration, the UAE method was superior and more efficient for extracting carotenoids from C. reticulata Blanco peels.
大量的废物,尤其是柑橘类水果的外层(果皮),在食用果肉后会产生,而果肉一直未被利用。通过回收这些废物的生物活性化合物来使其增值似乎很有趣。本研究旨在探讨超声辅助提取柑橘果皮中类胡萝卜素含量和抗氧化活性最高的最佳工艺条件。采用Box-Behnken试验设计的响应面法(RSM)优化了UAE提取类胡萝卜素的工艺条件。选取了正己烷浓度、温度和超声时间作为主要影响因素。结果显示,所有的自变量都影响了回答。正己烷浓度为60.76%,超声温度为36.45℃,超声时间为37.32 min。黄芪总类胡萝卜素含量(TCC)和总抗氧化活性(TAA)均高于浸渍法。综上所述,介质和提取参数(己烷浓度、温度、超声时间)对类胡萝卜素的回收率和抗氧化活性有显著影响。优化研究允许确定适当的条件,以最大限度地提高两种反应。与传统浸渍法相比,UAE法对黄皮类胡萝卜素的提取效率更高。
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引用次数: 0
Rethinking precautionary allergen labelling – Threshold doses, risk assessment approaches and analytical implications 重新思考预防性过敏原标签——阈值剂量、风险评估方法和分析意义
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-07-03 DOI: 10.1556/066.2023.00053
Z. Bugyi, G. Muskovics, S. Tömösközi
Food allergies became a major public health and food safety interest in the past decades as their prevalence is increasing, and their only available treatment is a strict elimination diet that necessitates appropriate food labelling regulations. While such regulations are available worldwide, most of them are not taking into account inadvertent allergen cross-contamination and they usually do not define threshold doses that could support the industry in their endeavour to provide reliable food labels for allergic consumers. This resulted in the proliferation of the “may contain” type precautionary allergen labelling (PAL), which is voluntary and is intended to warn consumers for potential unintended contamination with an otherwise undeclared allergen. As this kind of labelling is hardly ever based on actual risk assessment, it puts both the industry and the consumer into a difficult position. A promising tool towards the solution of this problem could be allergen threshold doses based on clinical data, which are becoming increasingly available. This review intends to present this process, the new ways of improved risk assessment it opens, and its implications for food analysis.
在过去的几十年里,随着食物过敏的流行率不断上升,食物过敏成为公众健康和食品安全的主要关注点,而他们唯一可用的治疗方法是严格的消除饮食,这需要适当的食品标签规定。虽然此类法规在世界各地都有,但大多数法规都没有考虑到无意中的过敏原交叉污染,通常也没有定义可以支持行业为过敏消费者提供可靠食品标签的阈值剂量。这导致了“可能含有”型预防性过敏原标签(PAL)的激增,该标签是自愿的,旨在警告消费者可能被未申报的过敏原意外污染。由于这种标签几乎从未基于实际风险评估,这使行业和消费者都陷入了困境。解决这一问题的一个有前途的工具可能是基于临床数据的过敏原阈值剂量,这些数据越来越可用。这篇综述旨在介绍这一过程,它开启的改进风险评估的新方法,以及它对食品分析的影响。
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引用次数: 0
Quality changes of Hoa Loc mangoes (Mangifera indica L.) during storage: Effect of chitosan-based nano-silver film coating 壳聚糖基纳米银膜涂层对华洛芒果贮藏品质的影响
IF 1.1 4区 农林科学 Q3 Agricultural and Biological Sciences Pub Date : 2023-06-22 DOI: 10.1556/066.2021.00163
H. Nguyen, L. Nguyen, V. D. Nguyen, Q.D. Duong, B. Nguyen
The aim of this study was to evaluate the possibility of storage of mangoes (Mangifera indica L.) coated with chitosan-based nano-silver films. Chitosan-based nano-silver films were first made using chitosan with three degrees of deacetylation of 70, 80, and 90%. The films were then used for coating the mango fruits. All coated mangoes and the control samples were stored at 12 °C in a cooling room. The respiration rate, ethylene production rate, weight loss, firmness, total soluble solids, total acid, vitamin C, total sugar, and change of peel colour were evaluated once every 5 days during the storage period. As observed, the coating using the chitosan-based nano-silver films did not induce significant negative alterations on most of the physical characteristics and chemical constituents of the fruits. In addition, the coating using those materials helped reduce the respiration and the ethylene gas production and retard the ripening process of mango fruits. The chitosan-based films with higher deacetylation degrees (CN80 and CN90) better moderated the respiration of mangoes. The coating using chitosan-based nano-silver films prolonged the shelf-life of mangoes (up to 35 days) compared to the control (less than 10 days).
本研究的目的是评估用壳聚糖基纳米银膜包裹芒果(Mangifera indica L.)储存的可能性。首次用脱乙酰度分别为70%、80%和90%的壳聚糖制备了壳聚糖基纳米银膜。然后用这些薄膜覆盖芒果果实。所有包衣芒果和对照样品都储存在12 在冷却室内温度为°C。在贮藏期间,每5天评估一次呼吸速率、乙烯生产速率、重量损失、硬度、总可溶性固形物、总酸、维生素C、总糖和果皮颜色的变化。如所观察到的,使用壳聚糖基纳米银膜的涂层对果实的大部分物理特性和化学成分没有引起显著的负面变化。此外,使用这些材料的涂层有助于减少呼吸和乙烯气体的产生,并延缓芒果果实的成熟过程。脱乙酰度较高的壳聚糖膜(CN80和CN90)能较好地调节芒果的呼吸。与对照组(不到10天)相比,使用壳聚糖基纳米银膜的涂层延长了芒果的保质期(长达35天)。
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引用次数: 0
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Acta Alimentaria
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