Background: Toxoplasma gondii, a globally distributed zoonotic obligate intracellular parasite, infects a wide array of mammals, including humans, sheep, and birds. As a unique sheep breed in southwestern China, Yunnan semi-fine wool sheep occupies an important position in animal husbandry in Zhaotong due to its strong adaptability, high reproductive rate, and excellent wool quality. Lambs infected with T. gondii are prone to neurological symptoms and growth retardation, while T. gondii infection in ewes can cause abortions, stillbirths, and deformities, thus affecting sheep reproduction and sheep product quality. Meanwhile, mutton and dairy products contaminated with T. gondii can become potential sources of human infection, potentially threatening public health and safety.
Method: To understand the T. gondii infection in semi-fine wool sheep in Zhaotong, Yunnan Province, 586 blood samples were collected and subjected to indirect hemagglutination assay (IHA) for T. gondii antibodies, and the infection-related factors were analyzed through cross-sectional analysis. In the meantime, nested PCR was conducted on a total of 217 samples collected from 31 rodents caught in and around the sheep breeding ground to test the T. gondii B1 gene in rodent tissues.
Results: A total of 94 sera tested positive for T. gondii antibodies, with a total positive rate of 16.04% (94/586) (95% CI: 14.77-20.89). Cross-sectional statistical analysis on factors related to semi-fine wool sheep infection rate, including sampling season, sex, age, and weight, suggested that age (< 6 months: 23.81%; 6-12 months: 11.74%; > 12 months: 15.83%) was a significant factor explaining the infection rate differences (P = 0.003 < 0.05, χ2 = 11.62, df = 2). Thus, age was considered a key risk factor for T. gondii infection in this study (odds ratio, OR = 2.35, 95% CI: 1.42-3.87). Nested PCR analysis on 217 (heart, liver, spleen, lung, kidney, brain, and muscle) tissues from the 31 rodents indicated that 11 tested positive. The total infection rate of rodents in and around the breeding ground was 35.48% (11/31), and 14 samples tested positive, with a positive infection rate of 6.45% (14/217).
Conclusion: The T. gondii infection rates of semi-fine wool sheep and rodents from their breeding environment in Zhaotong, Yunnan Province, were high, necessitating enhanced prevention, control, and treatment measures to ensure the healthy breeding of semi-fine wool sheep and veterinary public health and safety.
{"title":"Investigation of Toxoplasma gondii Infection in Yunnan Semi-fine Wool Sheep (Ovis aries) and wild Rodents in Yunnan, China.","authors":"Zhao Li, Wen-Jie Cheng, Cai-Qin Deng, Meng-Ling Deng, Hai-Bo Peng, Xing-Quan Zhu, Feng-Cai Zou","doi":"10.1007/s11686-024-00875-2","DOIUrl":"10.1007/s11686-024-00875-2","url":null,"abstract":"<p><strong>Background: </strong>Toxoplasma gondii, a globally distributed zoonotic obligate intracellular parasite, infects a wide array of mammals, including humans, sheep, and birds. As a unique sheep breed in southwestern China, Yunnan semi-fine wool sheep occupies an important position in animal husbandry in Zhaotong due to its strong adaptability, high reproductive rate, and excellent wool quality. Lambs infected with T. gondii are prone to neurological symptoms and growth retardation, while T. gondii infection in ewes can cause abortions, stillbirths, and deformities, thus affecting sheep reproduction and sheep product quality. Meanwhile, mutton and dairy products contaminated with T. gondii can become potential sources of human infection, potentially threatening public health and safety.</p><p><strong>Method: </strong>To understand the T. gondii infection in semi-fine wool sheep in Zhaotong, Yunnan Province, 586 blood samples were collected and subjected to indirect hemagglutination assay (IHA) for T. gondii antibodies, and the infection-related factors were analyzed through cross-sectional analysis. In the meantime, nested PCR was conducted on a total of 217 samples collected from 31 rodents caught in and around the sheep breeding ground to test the T. gondii B1 gene in rodent tissues.</p><p><strong>Results: </strong>A total of 94 sera tested positive for T. gondii antibodies, with a total positive rate of 16.04% (94/586) (95% CI: 14.77-20.89). Cross-sectional statistical analysis on factors related to semi-fine wool sheep infection rate, including sampling season, sex, age, and weight, suggested that age (< 6 months: 23.81%; 6-12 months: 11.74%; > 12 months: 15.83%) was a significant factor explaining the infection rate differences (P = 0.003 < 0.05, χ<sup>2</sup> = 11.62, df = 2). Thus, age was considered a key risk factor for T. gondii infection in this study (odds ratio, OR = 2.35, 95% CI: 1.42-3.87). Nested PCR analysis on 217 (heart, liver, spleen, lung, kidney, brain, and muscle) tissues from the 31 rodents indicated that 11 tested positive. The total infection rate of rodents in and around the breeding ground was 35.48% (11/31), and 14 samples tested positive, with a positive infection rate of 6.45% (14/217).</p><p><strong>Conclusion: </strong>The T. gondii infection rates of semi-fine wool sheep and rodents from their breeding environment in Zhaotong, Yunnan Province, were high, necessitating enhanced prevention, control, and treatment measures to ensure the healthy breeding of semi-fine wool sheep and veterinary public health and safety.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01Epub Date: 2024-08-10DOI: 10.1007/s11686-024-00876-1
Yuki Kita, Masato Nitta, Hiroshi Kajihara
Purpose: Molecular phylogenetics has been improving the acanthocephalan systematics, yet numerous taxa remain unexplored. The palaeacanthocephalan Metarhadinorhynchus Yamaguti, 1959 and its type species M. lateolabracis Yamaguti, 1959 are such to-be-explored taxa. We aim to refine (i) the systematic placement, (ii) the morphological circumscription, and (iii) the taxonomic components of the genus. We also aim to examine the taxonomic status of the species that have been assigned to the genus.
Methods: Morphological observations on newly collected specimens as well as the type material of Metarhadinorhynchus lateolabracis were conducted. Also, molecular phylogenetic analyses with maximum-likelihood method and Bayesian inference were performed based on freshly collected specimens. Nominal species that have at least once been assigned in Metarhadinorhynchus, as well as a related form, Gorgorhynchus lateolabri Yin and Wu, 1984, are taxonomically re-evaluated based on literature information.
Results: Our re-examination of the type material of M. lateolabracis revealed that the number of cement glands is six, instead of eight as described and illustrated in the original description. In the resulting phylogenetic tree, M. lateolabracis was nested in Isthmosacanthidae. Gorgorhynchoides Cable and Linderoth, 1963 was found to be a junior synonym of Metarhadinorhynchus. Taxonomic re-evaluations of six nominal species that have once belonged in Metarhadinorhynchus led to modifications of generic diagnoses for Indorhynchus Golvan, 1969 and Neotegorhynchus Lisitsyna, Xi, Orosová, Barčák, and Oros, 2022.
Conclusions: Metarhadinorhynchus has been assigned to Leptorhynchoididae (Echinorhynchida), but our study now locates it in Isthmosacanthidae (Polymorphida). We propose 13 new combinations of specific names in Metarhadinorhynchus and three in Indorhynchus. Metarhadinorhynchus lateolabri (Yin and Wu, 1984) comb. nov. may be synonymous with M. orientalis (Wang, 1966) comb. nov.
{"title":"Molecular Systematics and Type-material-based Redescription of Metarhadinorhynchus lateolabracis, With Taxonomic Revisions of Metarhadinorhynchus, Indorhynchus, and Neotegorhynchus (Acanthocephala: Palaeacanthocephala).","authors":"Yuki Kita, Masato Nitta, Hiroshi Kajihara","doi":"10.1007/s11686-024-00876-1","DOIUrl":"10.1007/s11686-024-00876-1","url":null,"abstract":"<p><strong>Purpose: </strong>Molecular phylogenetics has been improving the acanthocephalan systematics, yet numerous taxa remain unexplored. The palaeacanthocephalan Metarhadinorhynchus Yamaguti, 1959 and its type species M. lateolabracis Yamaguti, 1959 are such to-be-explored taxa. We aim to refine (i) the systematic placement, (ii) the morphological circumscription, and (iii) the taxonomic components of the genus. We also aim to examine the taxonomic status of the species that have been assigned to the genus.</p><p><strong>Methods: </strong>Morphological observations on newly collected specimens as well as the type material of Metarhadinorhynchus lateolabracis were conducted. Also, molecular phylogenetic analyses with maximum-likelihood method and Bayesian inference were performed based on freshly collected specimens. Nominal species that have at least once been assigned in Metarhadinorhynchus, as well as a related form, Gorgorhynchus lateolabri Yin and Wu, 1984, are taxonomically re-evaluated based on literature information.</p><p><strong>Results: </strong>Our re-examination of the type material of M. lateolabracis revealed that the number of cement glands is six, instead of eight as described and illustrated in the original description. In the resulting phylogenetic tree, M. lateolabracis was nested in Isthmosacanthidae. Gorgorhynchoides Cable and Linderoth, 1963 was found to be a junior synonym of Metarhadinorhynchus. Taxonomic re-evaluations of six nominal species that have once belonged in Metarhadinorhynchus led to modifications of generic diagnoses for Indorhynchus Golvan, 1969 and Neotegorhynchus Lisitsyna, Xi, Orosová, Barčák, and Oros, 2022.</p><p><strong>Conclusions: </strong>Metarhadinorhynchus has been assigned to Leptorhynchoididae (Echinorhynchida), but our study now locates it in Isthmosacanthidae (Polymorphida). We propose 13 new combinations of specific names in Metarhadinorhynchus and three in Indorhynchus. Metarhadinorhynchus lateolabri (Yin and Wu, 1984) comb. nov. may be synonymous with M. orientalis (Wang, 1966) comb. nov.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01Epub Date: 2024-08-17DOI: 10.1007/s11686-024-00891-2
Usman Ahmed, Lai Ti Gew, Ruqaiyyah Siddiqui, Naveed Ahmed Khan, Ahmad M Alharbi, Ayman Alhazmi, Ayaz Anwar
Purpose: The treatment of amoebic infections is often problematic, largely due to delayed diagnosis, amoebae transformation into resistant cyst form, and lack of availability of effective chemotherapeutic agents. Herein, we determined anti-Acanthamoeba castellanii properties of three metal oxide nanoparticles (TiO2, ZrO2, and Al2O3).
Methods: Amoebicidal assays were performed to determine whether metal oxide nanoparticles inhibit amoebae viability. Encystation assays were performed to test whether metal oxide nanoparticles inhibit cyst formation. By measuring lactate dehydrogenase release, cytotoxicity assays were performed to determine human cell damage. Hoechst 33342/PI staining was performed to determine programmed cell death (apoptosis) and necrosis in A. castellanii.
Results: TiO2-NPs significantly inhibited amoebae viability as observed through amoebicidal assays, as well as inhibited their phenotypic transformation as evident using encystation assays, and showed limited human cell damage as observed by measuring lactate dehydrogenase assays. Furthermore, TiO2-NPs altered parasite membranes and resulted in necrotic cell death as determined using double staining cell death assays with Hoechst33342/Propidium iodide (PI) observed through chromatin condensation. These findings suggest that TiO2-NPs offers a potential viable avenue in the rationale development of therapeutic interventions against Acanthamoeba infections.
{"title":"Metal Oxide Nanoparticles Exhibit Anti-Acanthamoeba castellanii Properties by Inducing Necrotic Cell Death.","authors":"Usman Ahmed, Lai Ti Gew, Ruqaiyyah Siddiqui, Naveed Ahmed Khan, Ahmad M Alharbi, Ayman Alhazmi, Ayaz Anwar","doi":"10.1007/s11686-024-00891-2","DOIUrl":"10.1007/s11686-024-00891-2","url":null,"abstract":"<p><strong>Purpose: </strong>The treatment of amoebic infections is often problematic, largely due to delayed diagnosis, amoebae transformation into resistant cyst form, and lack of availability of effective chemotherapeutic agents. Herein, we determined anti-Acanthamoeba castellanii properties of three metal oxide nanoparticles (TiO<sub>2</sub>, ZrO<sub>2</sub>, and Al<sub>2</sub>O<sub>3</sub>).</p><p><strong>Methods: </strong>Amoebicidal assays were performed to determine whether metal oxide nanoparticles inhibit amoebae viability. Encystation assays were performed to test whether metal oxide nanoparticles inhibit cyst formation. By measuring lactate dehydrogenase release, cytotoxicity assays were performed to determine human cell damage. Hoechst 33342/PI staining was performed to determine programmed cell death (apoptosis) and necrosis in A. castellanii.</p><p><strong>Results: </strong>TiO<sub>2</sub>-NPs significantly inhibited amoebae viability as observed through amoebicidal assays, as well as inhibited their phenotypic transformation as evident using encystation assays, and showed limited human cell damage as observed by measuring lactate dehydrogenase assays. Furthermore, TiO<sub>2</sub>-NPs altered parasite membranes and resulted in necrotic cell death as determined using double staining cell death assays with Hoechst33342/Propidium iodide (PI) observed through chromatin condensation. These findings suggest that TiO<sub>2</sub>-NPs offers a potential viable avenue in the rationale development of therapeutic interventions against Acanthamoeba infections.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01Epub Date: 2024-08-19DOI: 10.1007/s11686-024-00905-z
Fredrick O Obonyo, Ndichu Maingi, Samuel M Githigia, Kevin O Ochwedo, Anne A Owiti, Evans N Nyaboga
Purpose: The present study was conducted to determine the presence of Toxoplasma gondii in donkeys by molecular tests and genetic diversity analysis of the obtained DNA samples from central Kenya.
Method: A total of 363 blood samples were collected from donkeys in Meru and Kirinyaga Counties, and 96 samples that were previously seropositive for T. gondii using indirect ELISA were subjected to nested PCR based on the amplification of the internal transcribed spacer 1 (ITS-1) gene followed by DNA sequencing and phylogenetic analysis. Genotyping was performed on 15 selected positive samples using multilocus nested polymerase chain reaction restriction fragment length polymorphism (Mn-PCR-RFLP) with eight genetic markers ('SAG 2, 5'SAG 2, Alt. SAG 2, SAG 3, GRA 6, C29-2, BTUB and L358).
Results: Toxoplasma gondii DNA was detected in 36.5% (35/96) of the blood samples. The sequences obtained exhibited 98.2-99.5% homology with those deposited in GenBank. Phylogenetic analysis demonstrated that the obtained sequences are conserved and clustered with those of infecting animals from other regions of the world. Eighteen distinct T. gondii haplotypes were identified to be circulating in donkeys from central Kenya. The T. gondii DNA samples exhibited high haplotype diversity (Hd: 0.915) and limited genetic diversity (π = 0.01027). PCR-RFLP of T. gondii DNA-positive samples revealed three different genetic combinations that consisted of alleles I, II and III, indicating the dissemination of atypical genotypes.
Conclusion: This study demonstrated that T. gondii is widespread in donkeys from Kenya and could be a possible source of infection in humans. These findings are important for designing control strategies for this parasite to improve the livestock sector, which is one of the main sources of livelihood for farmers in Kenya.
目的:本研究旨在通过分子检测和对肯尼亚中部地区获得的 DNA 样本进行遗传多样性分析,确定驴体内是否存在弓形虫:方法:从梅鲁县和基里尼亚加县的驴子身上共采集了 363 份血样,其中 96 份血样曾用间接 ELISA 方法检测出弓形虫血清阳性,对其进行了基于内部转录间隔 1 (ITS-1) 基因扩增的巢式 PCR 检测,随后进行了 DNA 测序和系统发育分析。利用多焦点巢式聚合酶链反应限制性片段长度多态性(Mn-PCR-RFLP)和八个遗传标记('SAG 2、5'SAG 2、Alt. SAG 2、SAG 3、GRA 6、C29-2、BTUB 和 L358)对 15 个选定的阳性样本进行了基因分型:结果:36.5%(35/96)的血液样本中检测到弓形虫 DNA。获得的序列与 GenBank 中的序列有 98.2-99.5% 的同源性。系统进化分析表明,所获得的序列与世界其他地区感染动物的序列是一致的,并且是聚类的。在肯尼亚中部地区的驴中发现了18种不同的淋病双球菌单倍型。淋球菌 DNA 样本表现出较高的单倍型多样性(Hd:0.915)和有限的遗传多样性(π = 0.01027)。淋球菌 DNA 阳性样本的 PCR-RFLP 显示出三种不同的基因组合,包括等位基因 I、II 和 III,表明非典型基因型的传播:这项研究表明,淋病双球菌广泛存在于肯尼亚的驴中,可能是人类的一个传染源。这些发现对于设计这种寄生虫的控制策略以改善作为肯尼亚农民主要生计来源之一的畜牧业非常重要。
{"title":"First Report on the Molecular Detection and Genetic Characterization of Toxoplasma gondii From Donkeys in Kenya.","authors":"Fredrick O Obonyo, Ndichu Maingi, Samuel M Githigia, Kevin O Ochwedo, Anne A Owiti, Evans N Nyaboga","doi":"10.1007/s11686-024-00905-z","DOIUrl":"10.1007/s11686-024-00905-z","url":null,"abstract":"<p><strong>Purpose: </strong>The present study was conducted to determine the presence of Toxoplasma gondii in donkeys by molecular tests and genetic diversity analysis of the obtained DNA samples from central Kenya.</p><p><strong>Method: </strong>A total of 363 blood samples were collected from donkeys in Meru and Kirinyaga Counties, and 96 samples that were previously seropositive for T. gondii using indirect ELISA were subjected to nested PCR based on the amplification of the internal transcribed spacer 1 (ITS-1) gene followed by DNA sequencing and phylogenetic analysis. Genotyping was performed on 15 selected positive samples using multilocus nested polymerase chain reaction restriction fragment length polymorphism (Mn-PCR-RFLP) with eight genetic markers ('SAG 2, 5'SAG 2, Alt. SAG 2, SAG 3, GRA 6, C29-2, BTUB and L358).</p><p><strong>Results: </strong>Toxoplasma gondii DNA was detected in 36.5% (35/96) of the blood samples. The sequences obtained exhibited 98.2-99.5% homology with those deposited in GenBank. Phylogenetic analysis demonstrated that the obtained sequences are conserved and clustered with those of infecting animals from other regions of the world. Eighteen distinct T. gondii haplotypes were identified to be circulating in donkeys from central Kenya. The T. gondii DNA samples exhibited high haplotype diversity (Hd: 0.915) and limited genetic diversity (π = 0.01027). PCR-RFLP of T. gondii DNA-positive samples revealed three different genetic combinations that consisted of alleles I, II and III, indicating the dissemination of atypical genotypes.</p><p><strong>Conclusion: </strong>This study demonstrated that T. gondii is widespread in donkeys from Kenya and could be a possible source of infection in humans. These findings are important for designing control strategies for this parasite to improve the livestock sector, which is one of the main sources of livelihood for farmers in Kenya.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: The influence of two key factors, host length and infection site, on the host-parasite interaction in Ompok bimaculatus (Butter catfish) from Mukutmanipur Dam Lake, were investigated.
Methods: Present study involved 192 specimens of Ompok bimaculatus with varying body lengths, subjected to diverse statistical analyses. One-way analysis of variance (ANOVA) was performed for the parasite numbers for three groups (cestode, nematode and trematode). Subsequently, we conducted one-way permutational multivariate analysis of variance (PERMANOVA) followed by pairwise test to assess parasite numbers across three body sites (intestine, mesentery, and bodycavity), employing the Bray-Curtis index. Additionally, Principal Coordinate Analysis (PCoA) for the same dataset was performed using the same index. Linear regression analysis was performed for the fish length-cestode number, fish length-nematode number, fish length-trematode number and fish length-total parasite number.
Results: One-way ANOVA revealed no significant differences in parasite numbers among the three endo-helminth groups (cestode, nematode, and trematode). The results of PERMANOVA revealed significant differences in parasite numbers across the three body sites of the host fishes (groups) (F = 9.41, p = 0.0001). Pairwise tests further demonstrated significant differences between the intestine-mesentery, intestine-body-cavity, and mesentery-body-cavity. Additionally, Principal Coordinate Analysis (PCoA) unveiled a significant relationship between infection site and parasite number. However, linear regression analysis examining the relationship between fish length and parasite abundance indicated no significant associations.
Conclusions: Through a detailed exploration of the statistical analyses, we provide insights into the host-parasite interaction, elucidating both established knowledge and novel findings in fish parasitology.
{"title":"Unlocking the Biological Enigma: Influence of Host Length and Infection Site on Parasite Abundance in Ompok bimaculatus.","authors":"Jit Marick, Subha Shankar Mukherjee, Bhairab Kumar Patra, Anirban Ash","doi":"10.1007/s11686-024-00879-y","DOIUrl":"10.1007/s11686-024-00879-y","url":null,"abstract":"<p><strong>Purpose: </strong>The influence of two key factors, host length and infection site, on the host-parasite interaction in Ompok bimaculatus (Butter catfish) from Mukutmanipur Dam Lake, were investigated.</p><p><strong>Methods: </strong>Present study involved 192 specimens of Ompok bimaculatus with varying body lengths, subjected to diverse statistical analyses. One-way analysis of variance (ANOVA) was performed for the parasite numbers for three groups (cestode, nematode and trematode). Subsequently, we conducted one-way permutational multivariate analysis of variance (PERMANOVA) followed by pairwise test to assess parasite numbers across three body sites (intestine, mesentery, and bodycavity), employing the Bray-Curtis index. Additionally, Principal Coordinate Analysis (PCoA) for the same dataset was performed using the same index. Linear regression analysis was performed for the fish length-cestode number, fish length-nematode number, fish length-trematode number and fish length-total parasite number.</p><p><strong>Results: </strong>One-way ANOVA revealed no significant differences in parasite numbers among the three endo-helminth groups (cestode, nematode, and trematode). The results of PERMANOVA revealed significant differences in parasite numbers across the three body sites of the host fishes (groups) (F = 9.41, p = 0.0001). Pairwise tests further demonstrated significant differences between the intestine-mesentery, intestine-body-cavity, and mesentery-body-cavity. Additionally, Principal Coordinate Analysis (PCoA) unveiled a significant relationship between infection site and parasite number. However, linear regression analysis examining the relationship between fish length and parasite abundance indicated no significant associations.</p><p><strong>Conclusions: </strong>Through a detailed exploration of the statistical analyses, we provide insights into the host-parasite interaction, elucidating both established knowledge and novel findings in fish parasitology.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01Epub Date: 2024-08-21DOI: 10.1007/s11686-024-00895-y
Germán Augusto Murrieta Morey, Hilmer A Dávila, Mariana Vásquez Arimuya, Alana Lislea de Sousa, Celso L Cruces, Jhon D Chero
Introduction: The present study describes three new dactylogyrid species infecting the gill filaments of cichlid fishes (Cichliformes: Cichlidae) from the Amazon basin, Peru: Sciadicleithrum amazoniensis n. sp. on Biotodoma cupido (Heckel, 1840), and Sciadicleithrum feliciajaramae n. sp. and Sciadicleithrum souzatecci n. sp. on Bujurquina peregrinabunda Kullander, 1986.
Materials and methods: Some monogeneans were stained with Gomori's trichrome and mounted in Canada Balsam to determine internal soft structures. Others were cleared in Hoyer's medium for the study of sclerotized structures. Drawings were made using a drawing tube and a microprojector.
Results: Sciadicleithrum amazoniensis n. sp. is characterized by the presence of a male copulatory organ (MCO) with a coil of approximately 2 counterclockwise rings, an accessory piece articulated to base of the MCO with an expanded proximal end and a bifurcated distal end, and a sinistral vaginal aperture. Sciadicleithrum feliciajaramae n. sp. can be differentiated from all its congeners by its J-shaped MCO with about half a counterclockwise loop and a rod-shaped accessory piece articulated to the base of the MCO, with the distal end bent. Sciadicleithrum souzatecci n. sp. differs from all other members of Sciadicleithrum by having an elongated MCO with about a clockwise loop and a funnel-shaped base. Additionally, Sciadicleithrum souzatecci n. sp. is characterized by its weakly sclerotized, C-shaped accessory piece with a robust middle process.
Conclusions: Present findings are added to the other 26 species previously known in Sciadicleithrum.This is the first data on the parasites of B. peregrinabunda.
简介:本研究描述了感染秘鲁亚马逊河流域慈鲷(慈鲷形目:Cichlidae)鳃丝的三种新的dactylogyrid物种:Sciadicleithrum amazoniensis n. sp. on Biotodoma cupido (Heckel, 1840), and Sciadicleithrum feliciajaramae n. sp. and Sciadicleithrum souzatecci n. sp. on Bujurquina peregrinabunda Kullander, 1986:用 Gomori 三色染色剂对一些单体进行染色,并用加拿大香脂装裱,以确定内部软结构。另一些则在霍耶氏培养基中清洗,以研究硬质结构。使用绘图管和微型投影仪进行绘图:Sciadicleithrum amazoniensis n.sp.的特征是有一个雄性交配器官(MCO),该器官由大约两个逆时针方向的环形线圈组成,一个附件与 MCO 的基部衔接,具有膨大的近端和分叉的远端,以及一个窦状阴道孔。Sciadicleithrum feliciajaramae n. sp.可与其所有同类区分开来,因为它的 MCO 呈 J 形,约有半个逆时针环,一个棒状的附属片与 MCO 基部相连,远端弯曲。Sciadicleithrum souzatecci n. sp.与 Sciadicleithrum 的所有其他成员都不同,其拉长的 MCO 大约有一个顺时针环,基部呈漏斗状。此外,Sciadicleithrum souzatecci n. sp.的特征还包括其硬质化程度较弱的 C 形附属片,中间有一个粗壮的突起:目前的发现是对之前已知的 26 种 Sciadicleithrum 的补充。
{"title":"Three New Species of Sciadicleithrum (Monogenoidea, Dactylogyridae) Parasitizing Cichlid Fishes (Cichliformes: Cichlidae) in the Northeastern Peru.","authors":"Germán Augusto Murrieta Morey, Hilmer A Dávila, Mariana Vásquez Arimuya, Alana Lislea de Sousa, Celso L Cruces, Jhon D Chero","doi":"10.1007/s11686-024-00895-y","DOIUrl":"10.1007/s11686-024-00895-y","url":null,"abstract":"<p><strong>Introduction: </strong>The present study describes three new dactylogyrid species infecting the gill filaments of cichlid fishes (Cichliformes: Cichlidae) from the Amazon basin, Peru: Sciadicleithrum amazoniensis n. sp. on Biotodoma cupido (Heckel, 1840), and Sciadicleithrum feliciajaramae n. sp. and Sciadicleithrum souzatecci n. sp. on Bujurquina peregrinabunda Kullander, 1986.</p><p><strong>Materials and methods: </strong>Some monogeneans were stained with Gomori's trichrome and mounted in Canada Balsam to determine internal soft structures. Others were cleared in Hoyer's medium for the study of sclerotized structures. Drawings were made using a drawing tube and a microprojector.</p><p><strong>Results: </strong>Sciadicleithrum amazoniensis n. sp. is characterized by the presence of a male copulatory organ (MCO) with a coil of approximately 2 counterclockwise rings, an accessory piece articulated to base of the MCO with an expanded proximal end and a bifurcated distal end, and a sinistral vaginal aperture. Sciadicleithrum feliciajaramae n. sp. can be differentiated from all its congeners by its J-shaped MCO with about half a counterclockwise loop and a rod-shaped accessory piece articulated to the base of the MCO, with the distal end bent. Sciadicleithrum souzatecci n. sp. differs from all other members of Sciadicleithrum by having an elongated MCO with about a clockwise loop and a funnel-shaped base. Additionally, Sciadicleithrum souzatecci n. sp. is characterized by its weakly sclerotized, C-shaped accessory piece with a robust middle process.</p><p><strong>Conclusions: </strong>Present findings are added to the other 26 species previously known in Sciadicleithrum.This is the first data on the parasites of B. peregrinabunda.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01Epub Date: 2024-08-20DOI: 10.1007/s11686-024-00883-2
Jahnvi Jakhan, Loick Pradel Kojom Foko, Geetika Narang, Vineeta Singh
Purpose: Primaquine (PQ) is recommended for radical cure of Plasmodium vivax (Pv) malaria, but its utilization is still limited due to high risk of severe haemolytic anaemia in patients with glucose-6-phosphate dehydrogenase deficiency (G6PD-d). The aim of the present study is to assess the different genotypic variants leading to G6PD-d in Delhi and Goa regions of India.
Methods: A total of 46 samples (34 retrospective Pv-mono-infected samples and 12 Pv-uninfected samples) were included in the study. Various genetic variants leading to G6PD-d were analysed by PCR amplification and DNA sequencing of different targeted exons of G6PD gene.
Results: Molecular analysis showed presence of four mutations in study population viz. 1311 C > T, 34.1% & IVSXI 93T > C, 45.5% and two novel mutations 1388G > T, 2.3% and 1398 C > T, 2.3% (silent mutation). The bioinformatics and computational analysis demonstrate that the slight conformational changes caused by R643L mutation in protein are deleterious in nature.
Conclusion: The observed mutations do not clarify the role or association between G6PD-d and Pv-infected cases. Further investigation is required in order to fully comprehend and analyse the precise role of these mutations with context to malaria infections.
{"title":"Glucose-6-phosphate Dehydrogenase Variants: Analysing in Indian Plasmodium vivax Patients.","authors":"Jahnvi Jakhan, Loick Pradel Kojom Foko, Geetika Narang, Vineeta Singh","doi":"10.1007/s11686-024-00883-2","DOIUrl":"10.1007/s11686-024-00883-2","url":null,"abstract":"<p><strong>Purpose: </strong>Primaquine (PQ) is recommended for radical cure of Plasmodium vivax (Pv) malaria, but its utilization is still limited due to high risk of severe haemolytic anaemia in patients with glucose-6-phosphate dehydrogenase deficiency (G6PD-d). The aim of the present study is to assess the different genotypic variants leading to G6PD-d in Delhi and Goa regions of India.</p><p><strong>Methods: </strong>A total of 46 samples (34 retrospective Pv-mono-infected samples and 12 Pv-uninfected samples) were included in the study. Various genetic variants leading to G6PD-d were analysed by PCR amplification and DNA sequencing of different targeted exons of G6PD gene.</p><p><strong>Results: </strong>Molecular analysis showed presence of four mutations in study population viz. 1311 C > T, 34.1% & IVSXI 93T > C, 45.5% and two novel mutations 1388G > T, 2.3% and 1398 C > T, 2.3% (silent mutation). The bioinformatics and computational analysis demonstrate that the slight conformational changes caused by R643L mutation in protein are deleterious in nature.</p><p><strong>Conclusion: </strong>The observed mutations do not clarify the role or association between G6PD-d and Pv-infected cases. Further investigation is required in order to fully comprehend and analyse the precise role of these mutations with context to malaria infections.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01Epub Date: 2024-05-31DOI: 10.1007/s11686-024-00855-6
Narges Shirzad Saghavaz, Mohammad Haseli
Purpose: The members of the tetraphyllidean genus Calliobothrium exhibit a high degree of host specificity in the shark genus Mustelus. In the Indian Ocean, where M. mosis occurs dominantly, there is no information on Calliobothrium. The purpose of this study was the evaluation of the Calliobothrium species in M. mosis in the Persian Gulf, northwestern Indian Ocean.
Methods: Nine specimens of Mustelus mosis were caught from the Persian Gulf. The tapeworms isolated were stained, measured, and the line drawings were made.
Results: Calliobothrium narsisae n. sp. is described and differs from C. creeveyae in the intact, rather than the three-lobed, posterior bothridial margins, from C. tylotocephalum in possessing three, rather than one, suckers per bothridial muscular pad, from C. nodosum in possessing the elongated, rather than robust and thorn-like, hooks, from C. shirozame in a maximum number of four, rather than three, dorsal and four, rather than three, ventral laciniations along the strobila, and from its other congeners in possessing three, rather than two, dorsal and three, rather than two, ventral laciniations in the anteriormost immature proglottids. A key to the species of the genus is presented and the proglottisation process, by the germinative zone placed in the terminal part of the worm at least in some stages of growth to maturity, is discussed.
Conclusion: The description of C. narsisae n. sp., which brings the total number of valid species of Calliobothrium to 10, also indicates that the germinative zone is not always at the anterior to strobila and in this new species it is placed in the terminal part of the worm at least in some stages of growth to maturity.
目的:Calliobothrium 四鳃鳗属的成员在鼬鲨属中表现出高度的宿主特异性。在印度洋,M. mosis 主要出现在这里,但没有关于 Calliobothrium 的信息。本研究的目的是评估印度洋西北部波斯湾的鼬鲨中的 Calliobothrium 种类:方法:从波斯湾捕获了九条贻贝标本。对分离出的绦虫进行染色、测量并绘制线图:与 C. creeveyae 的不同之处在于两鳃后缘是完整的而不是三裂的;与 C. tylotocephalum 的不同之处在于每两鳃肌垫有三个而不是一个吸盘;与 C. nodosum 的不同之处在于钩子是拉长的而不是粗壮的刺状;与 C. shirozame 的不同之处在于钩子的最大数目是 1,而不是 1。shirozame 的最大区别在于茎叶背侧裂片最多为 4 个(而非 3 个),腹侧裂片最多为 4 个(而非 3 个);与其他同属种的区别在于最前端的未成熟原叶背侧裂片最多为 3 个(而非 2 个),腹侧裂片最多为 3 个(而非 2 个)。本文给出了该属物种的检索表,并讨论了至少在生长至成熟的某些阶段,胚芽区位于虫体末端的原基化过程:对 C. narsisae n. sp.的描述使 Calliobothrium 的有效物种总数达到 10 个,同时还表明发芽区并不总是位于茎叶的前部,在该新物种中,至少在生长至成熟的某些阶段,发芽区位于虫体的末端。
{"title":"Calliobothrium Narsisae n. sp. (Tetraphyllidea: Calliobothriidae) from the Triakid Shark Mustelus mosis Hemprich and Ehrenberg from the Persian Gulf, with a Note on Its Specific Proglottisation Process and a Key to the Species of the Genus.","authors":"Narges Shirzad Saghavaz, Mohammad Haseli","doi":"10.1007/s11686-024-00855-6","DOIUrl":"10.1007/s11686-024-00855-6","url":null,"abstract":"<p><strong>Purpose: </strong>The members of the tetraphyllidean genus Calliobothrium exhibit a high degree of host specificity in the shark genus Mustelus. In the Indian Ocean, where M. mosis occurs dominantly, there is no information on Calliobothrium. The purpose of this study was the evaluation of the Calliobothrium species in M. mosis in the Persian Gulf, northwestern Indian Ocean.</p><p><strong>Methods: </strong>Nine specimens of Mustelus mosis were caught from the Persian Gulf. The tapeworms isolated were stained, measured, and the line drawings were made.</p><p><strong>Results: </strong>Calliobothrium narsisae n. sp. is described and differs from C. creeveyae in the intact, rather than the three-lobed, posterior bothridial margins, from C. tylotocephalum in possessing three, rather than one, suckers per bothridial muscular pad, from C. nodosum in possessing the elongated, rather than robust and thorn-like, hooks, from C. shirozame in a maximum number of four, rather than three, dorsal and four, rather than three, ventral laciniations along the strobila, and from its other congeners in possessing three, rather than two, dorsal and three, rather than two, ventral laciniations in the anteriormost immature proglottids. A key to the species of the genus is presented and the proglottisation process, by the germinative zone placed in the terminal part of the worm at least in some stages of growth to maturity, is discussed.</p><p><strong>Conclusion: </strong>The description of C. narsisae n. sp., which brings the total number of valid species of Calliobothrium to 10, also indicates that the germinative zone is not always at the anterior to strobila and in this new species it is placed in the terminal part of the worm at least in some stages of growth to maturity.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141178649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01Epub Date: 2024-08-20DOI: 10.1007/s11686-024-00887-y
Rahşan Koç Akpınar, Ali Tümay Gürler, Cenk Soner Bölükbaş, Selma Kaya, Serhat Arslan, Coşkun Aydın, Şakir Önder Türlek, Sema Nur Çelik, Ayşen Beyazıt, Taraneh Öncel, Ufuk Erol, Ayşe Türkan Çiftci, Zekai Bastem, Hasan Hüseyin Ünal, Mesut Şenel, Arif Bozdeveci, Şengül Alpay Karaoğlu, Murat Yaldız, Gökhan Güven, Bilal Küçükoğlu, Mitat Kurt
Purpose: Nosemosis is a disease that infects both Western honeybees (Apis mellifera L.) and Asian honeybees (Apis cerana) and causes colony losses and low productivity worldwide. In order to control nosemosis, it is important to determine the distribution and prevalence of this disease agent in a particular region. For this purpose, a national study was conducted to assess the prevalence of Nosema ceranae and N. apis throughout Türkiye, to perform network analyses of the parasites, and to determine the presence of nosemosis.
Methods: In this study which aimed to assess the prevalence of N. apis and N. ceranae in different colony types and regions where beekeeping is intensive in Türkiye, specimens were collected from hives with no clinical signs.
Results: A total of 1194 Western honeybee colonies in 400 apiaries from 40 provinces of Türkiye were examined by microscopic and molecular techniques. Nosemosis was found in all of 40 provinces. The mean prevalence ratio was 64.3 ± 3.0, with 95% CI in apiaries and 40.5 ± 2.9, 95% CI in hives. Nosema ceranae DNA was detected in all of positive hives, while N. ceranae and N. apis co-infection was detected in only four colonies.
Conclusion: This study showed that nosemosis has spread to all provinces, and it is common in every region of Türkiye. All of the N. ceranae or N. apis samples examined were 100% identical within themselves. Network analysis showed that they were within largest haplotype reported worldwide.
{"title":"Prevalence and Phylogenetic Network Analysis of Nosema apis and Nosema ceranae Isolates from Honeybee Colonies in Türkiye.","authors":"Rahşan Koç Akpınar, Ali Tümay Gürler, Cenk Soner Bölükbaş, Selma Kaya, Serhat Arslan, Coşkun Aydın, Şakir Önder Türlek, Sema Nur Çelik, Ayşen Beyazıt, Taraneh Öncel, Ufuk Erol, Ayşe Türkan Çiftci, Zekai Bastem, Hasan Hüseyin Ünal, Mesut Şenel, Arif Bozdeveci, Şengül Alpay Karaoğlu, Murat Yaldız, Gökhan Güven, Bilal Küçükoğlu, Mitat Kurt","doi":"10.1007/s11686-024-00887-y","DOIUrl":"10.1007/s11686-024-00887-y","url":null,"abstract":"<p><strong>Purpose: </strong>Nosemosis is a disease that infects both Western honeybees (Apis mellifera L.) and Asian honeybees (Apis cerana) and causes colony losses and low productivity worldwide. In order to control nosemosis, it is important to determine the distribution and prevalence of this disease agent in a particular region. For this purpose, a national study was conducted to assess the prevalence of Nosema ceranae and N. apis throughout Türkiye, to perform network analyses of the parasites, and to determine the presence of nosemosis.</p><p><strong>Methods: </strong>In this study which aimed to assess the prevalence of N. apis and N. ceranae in different colony types and regions where beekeeping is intensive in Türkiye, specimens were collected from hives with no clinical signs.</p><p><strong>Results: </strong>A total of 1194 Western honeybee colonies in 400 apiaries from 40 provinces of Türkiye were examined by microscopic and molecular techniques. Nosemosis was found in all of 40 provinces. The mean prevalence ratio was 64.3 ± 3.0, with 95% CI in apiaries and 40.5 ± 2.9, 95% CI in hives. Nosema ceranae DNA was detected in all of positive hives, while N. ceranae and N. apis co-infection was detected in only four colonies.</p><p><strong>Conclusion: </strong>This study showed that nosemosis has spread to all provinces, and it is common in every region of Türkiye. All of the N. ceranae or N. apis samples examined were 100% identical within themselves. Network analysis showed that they were within largest haplotype reported worldwide.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01Epub Date: 2024-08-15DOI: 10.1007/s11686-024-00874-3
Emily Andressa Santos Lima, Diefrey Ribeiro Campos, Eduardo Fellipe Melo Santos Soares, Anna Beatriz Ribeiro Fortunato, Taynara Monsores E Silva, Nayana de Figueiredo Pereira, Douglas Siqueira de Almeida Chaves, Yara Peluso Cid, Katherina Coumendouros
Purpose: The flea Ctenocephalides felis (Siphonaptera: Pulicidae), parasitizes dogs and cats globally, acting as a vector for various pathogens affecting both animals and humans. Growing interest in environmentally friendly, plant-based products prompted this study. The aim of the study was to determine the chemical composition of essential oils (EOs) from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea, assessing their insecticidal and repellent properties, determining lethal concentrations (LC50 and LC90), and evaluating residual efficacy in vitro against Ctenocephalides felis felis.
Methods: Gas Chromatography with Flame Ionization Detector analyzed EO composition. In vitro tests involved preparing EO solutions at various concentrations. Ten specimens from each life stage (egg, larva, pupa, adult) were used for insecticidal activity assessment. Adulticidal activity was assessed using 10 cm2 filter paper strip, each treated with 0.200 mL of the test solution. Immature stages activities were evaluated using 23.76 cm2 discs of the same filter paper, each treated with 0.470 mL of the test solution. Mortality percentage was calculated using (number of dead insects × 100) / number of incubated insects. Probit analysis calculated LC50 values with a 95% confidence interval.
Results: Major EO constituents were β-caryophyllene (EOCR), linalool (EOLH), linalyl acetate (EOSS), and limonene (EOCP). LC50 values were obtained for all stages except for the essential oil of C. paradisi. All oils showed repellent activity at 800 μg/cm2. OECR exhibited greater residual efficacy.
Conclusion: Each EO demonstrated superior insecticidal activity against specific C. felis felis stages.
{"title":"Insecticidal and Repellent Activity of Essential Oils from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea Against Immature and Adult Stages of Ctenocephalides felis felis.","authors":"Emily Andressa Santos Lima, Diefrey Ribeiro Campos, Eduardo Fellipe Melo Santos Soares, Anna Beatriz Ribeiro Fortunato, Taynara Monsores E Silva, Nayana de Figueiredo Pereira, Douglas Siqueira de Almeida Chaves, Yara Peluso Cid, Katherina Coumendouros","doi":"10.1007/s11686-024-00874-3","DOIUrl":"10.1007/s11686-024-00874-3","url":null,"abstract":"<p><strong>Purpose: </strong>The flea Ctenocephalides felis (Siphonaptera: Pulicidae), parasitizes dogs and cats globally, acting as a vector for various pathogens affecting both animals and humans. Growing interest in environmentally friendly, plant-based products prompted this study. The aim of the study was to determine the chemical composition of essential oils (EOs) from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea, assessing their insecticidal and repellent properties, determining lethal concentrations (LC50 and LC90), and evaluating residual efficacy in vitro against Ctenocephalides felis felis.</p><p><strong>Methods: </strong>Gas Chromatography with Flame Ionization Detector analyzed EO composition. In vitro tests involved preparing EO solutions at various concentrations. Ten specimens from each life stage (egg, larva, pupa, adult) were used for insecticidal activity assessment. Adulticidal activity was assessed using 10 cm<sup>2</sup> filter paper strip, each treated with 0.200 mL of the test solution. Immature stages activities were evaluated using 23.76 cm<sup>2</sup> discs of the same filter paper, each treated with 0.470 mL of the test solution. Mortality percentage was calculated using (number of dead insects × 100) / number of incubated insects. Probit analysis calculated LC50 values with a 95% confidence interval.</p><p><strong>Results: </strong>Major EO constituents were β-caryophyllene (EOCR), linalool (EOLH), linalyl acetate (EOSS), and limonene (EOCP). LC50 values were obtained for all stages except for the essential oil of C. paradisi. All oils showed repellent activity at 800 μg/cm<sup>2</sup>. OECR exhibited greater residual efficacy.</p><p><strong>Conclusion: </strong>Each EO demonstrated superior insecticidal activity against specific C. felis felis stages.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}