Acta Parasitologica最新文献_第6页

Molecular Survey and Phylogenetic Analyses of Canine Hemoplasma Species in Different Parts of Türkiye 犬不同部位血浆种类的分子调查与系统发育分析

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01174-0

Ufuk Erol, Omer Faruk Sahin, Melih Gazi Genc, Husnu Furkan Sakar, Kursat Altay

Purpose

Canine hemoplasma species, Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum, have been identified in various parts of the world. These pathogens can lead to mild to severe infections, even death, in dogs. To date, several pathogens caused clinical infection in dog have been detected in Türkiye, but limited data are present about canine hemoplasma species in the country. The aim of this study was to investigate canine hemoplasma species in different parts of Türkiye, and to perform phylogenetic analyses of positive samples.

Methods

In the current work, 300 dog blood samples were obtained from different parts of Türkiye. DNA extraction was obtained from blood samples with a commercial kit. The obtained DNA was researched in terms of the presence of canine hemoplasma species with species-specific PCR assay. Partial parts of 16S rRNA gene of randomly selected six samples, three positive samples for each pathogen, were sequenced.

Results

PCR assay revealed that 3.67% of animals (11/300) were infected with canine hemoplasma species. The prevalence of M. haemocanis and Ca. Mycoplasma haematoparvum was determined as 2% (6/300) and 0.67% (2/300), respectively. Co-infection rate was 1% (3/300). After DNA sequence analyses obtained consensus sequences were uploaded to the GenBank under accession numbers: PV798019-PV798021 (M. haemocanis) and PV798022-PV798024 (Ca. Mycoplasma haematoparvum). BLAST analysis revealed 98.44–100% nucleotide similarity between M. haemocanis isolates identified in this work and other M. haemocanis isolates. The 97.41–100% nucleotide identities were seen between our Ca. Mycoplasma haematoparvum isolates and other Ca. Mycoplasma haematoparvum isolates present in the GenBank.

Conclusion

Considering that these two species may cause clinical infection in both animals and humans, it is thought that veterinarians and physicians living in the region should take the necessary precautions to reduce the harmful effects of M. haemocanis and Ca. Mycoplasma haematoparvum.

目的：在世界各地已发现了血支原体和候选血支原体。这些病原体可以导致狗轻微到严重的感染，甚至死亡。迄今为止，已在刚果民主共和国检测到几种引起犬临床感染的病原体，但关于该国犬血浆种类的数据有限。本研究的目的是调查犬基耶犬不同部位的血浆种类，并对阳性样本进行系统发育分析。方法采集犬犬基耶氏体不同部位血样300份。使用商用试剂盒从血液样本中提取DNA。用犬种特异性PCR法对所获得的DNA进行了研究。随机选取6份样本，每种病原体3份阳性样本，对16S rRNA基因进行部分测序。结果spcr检测结果显示，3.67%（11/300）的动物感染犬血原体。血球支原体和血球支原体阳性率分别为2%（6/300）和0.67%（2/300）。合并感染率为1%（3/300）。DNA序列分析获得一致结果后，将序列上传到GenBank，登录号为PV798019-PV798021 （haemocanis）和PV798022-PV798024 （Ca. Mycoplasma haematoparvum）。BLAST分析显示，本研究鉴定的血念珠菌分离株与其他血念珠菌分离株核苷酸相似性为98.44-100%。我们分离的血疟支原体与GenBank中其他分离的血疟支原体核苷酸同源性为97.41-100%。结论考虑到这两种动物均可引起动物和人的临床感染，认为该地区的兽医和医生应采取必要的预防措施，以减少血马支原体和血疟支原体的危害。

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引用次数: 0

De Novo Transcriptomic Response of Pirenella cingulata (Gastropoda) Infected with Acanthotrema tridactyla (Trematoda) 三趾棘虫（棘足目）感染带孔皮螺旋体（腹足目）的从头转录组学反应

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01175-z

Faruq Pathan, Ranjana Yadav, Mandar Nanajkar

Purpose

Pirenella cingulata- a snail from an intertidal mudflat serves as the first intermediate host of digenean trematode parasites. These trematode parasites castrate their host and alter the host's physiology for their own success and proliferation. In this study, we investigate the interaction between Pirenella cingulata and the trematode Acanthotrema tridactyla using a de novo transcriptomic approach.

Methods

RNA was extracted from both infected and non-infected Pirenella cingulata. High-throughput RNA sequencing was performed, and only healthy individuals were used for de novo assembly of the transcriptome. Furthermore, the transcripts were annotated, followed by the identification of differentially expressed genes using the DESeq2 package.

Results

We have identified 91 differentially expressed host genes that mostly suppress immune responses, altering metabolic and cytoskeletal-related functions. Downregulation of immune-related genes, including Cathepsin L, beta 1,3-glucan binding protein, scavenger receptors, and histone 3 (H3) suggest immune suppression. Gluconeogenesis, a key metabolic pathway, was compromised by downregulating the activity of alkaline phosphatase and lipase. Gastropods mediate immune-related ‘oxidative burst’ by generation of ROS- a mechanism to cope with parasites, which trematodes countered by downregulating xanthine hydrogenase/oxidase. Cytoskeletal proteins like actins was downregulated, used for encapsulation and phagocytosis, implying a compromised immune system.

Conclusion

This study provides insights into host-parasite interaction with the understanding of the immune functional response, metabolic and cytoskeletal-related processes of parasitised gastropods.

目的：潮间带泥滩蜗牛钩带螺是地沟吸虫寄生虫的第一中间寄主。这些吸虫寄生虫阉割宿主，并改变宿主的生理机能，以获得自身的成功和增殖。在这项研究中，我们利用一种新的转录组学方法研究了钩带Pirenella和三趾棘吸虫之间的相互作用。方法分别从感染和未感染的带状皮雷纳菌中提取srna。进行了高通量RNA测序，并且仅使用健康个体进行转录组的从头组装。此外，对转录本进行注释，随后使用DESeq2包鉴定差异表达基因。结果我们鉴定了91个差异表达的宿主基因，这些基因大多抑制免疫反应，改变代谢和细胞骨骼相关功能。免疫相关基因，包括组织蛋白酶L、β 1,3-葡聚糖结合蛋白、清道夫受体和组蛋白3 （H3）的下调提示免疫抑制。糖异生是一个关键的代谢途径，通过下调碱性磷酸酶和脂肪酶的活性而受到损害。腹足类动物通过产生ROS介导免疫相关的“氧化爆发”，这是一种应对寄生虫的机制，寄生虫通过下调黄嘌呤氢化酶/氧化酶来应对。细胞骨架蛋白如肌动蛋白被下调，用于包封和吞噬，这意味着免疫系统受损。结论本研究为了解腹足类寄生虫的免疫功能反应、代谢和细胞骨骼相关过程提供了新的思路。

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引用次数: 0

Correction: Prevalence and Phylogenetic Network Analysis of Nosema apis and Nosema ceranae Isolates from Honeybee Colonies in Türkiye 修正：<s:1>基耶蜜蜂群体中蜜蜂微虫和蜜蜂微虫分离株的流行率和系统发育网络分析

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01102-2

Rahşan Koç Akpınar, Ali Tümay Gürler, Cenk Soner Bölükbaş, Selma Kaya, Serhat Arslan, Coşkun Aydın, Şakir Önder Türlek, Sema Nur Çelik, Ayşen Beyazıt, Taraneh Öncel, Ufuk Erol, Ayşe Türkan Çiftci, Zekai Bastem, Hasan Hüseyin Ünal, Mesut Şenel, Arif Bozdeveci, Şengül Alpay Karaoğlu, Murat Yaldız, Gökhan Güven, Bilal Küçükoğlu, Mitat Kurt

引用次数: 0

The Culture Technology for the Transforming Parasite-Theileria Annulata Schizont-Infected Cells: Past–Present–Perspective 转化寄生物-环芽孢杆菌分裂感染细胞的培养技术：过去-现在的观点

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01173-1

Quanying Ma, Yuan Han, Yuqing Ma, Xiaoqiang Wang, Zhi Li

Purpose

The cell culture technology for Theileria annulata schizont-infected cells plays a vital role in basic research and control of bovine theileriosis. T. annulata is an intracellular parasite transmitted by some species of ticks.

Methods

Unlike other Apicomplexa parasites, T. annulata can infect and transform host B cells, dendritic cells and macrophages, further induce the transformed cells yield some cancer-like phenotypes. The attenuated parasites were obtained through in vitro culture technology, which can effectively prevent T. annulata infection.

Results

The cell culture has evolved from initial adherent culture to rolling bottle culture and then to suspension culture at present. In addition, the suspension culture has also been upgraded from basic serum-containing culture to serum-free culture.

Conclusion

This paper highlights the past, present and perspective about the cell culture technology with T. annulata schizont-infected cells, which will strongly underpin application of T. annulata vaccines and control of the parasite in the future.

目的建立牛环芽孢杆菌分裂感染细胞培养技术，对牛环芽孢杆菌病的基础研究和防治具有重要意义。环锥虫是一种由某些种类的蜱虫传播的细胞内寄生虫。方法与其他顶复合体寄生物一样，环虫可以感染和转化宿主B细胞、树突状细胞和巨噬细胞，并诱导转化后的细胞产生一些类似癌症的表型。通过体外培养技术获得了能有效预防环虫感染的减毒疟原虫。结果细胞培养经历了从最初的贴壁培养到滚瓶培养，再到目前的悬浮培养。此外，悬浮培养也从基本的含血清培养升级为无血清培养。结论综述了环虫裂殖细胞培养技术的历史、现状和发展趋势，为环虫疫苗的应用和今后环虫的防治奠定了基础。

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引用次数: 0

Epidemiological, Morphometric, and Genetic Analysis of Pediculus humanus capitis (Head Lice) in Pakistan: Evidence of Regional Diversity and Global Admixture 巴基斯坦头虱的流行病学、形态计量学和遗传分析：区域多样性和全球混合的证据

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01148-2

Muhammad Ilyas, Ayesha Gulzar, Tayyaba Shan, Fazeela Manzoor, Uzma Bibi, Maria Komal, Sabika Firasat, Kiran Afshan

Purpose

Pediculus humanus capitis poses a significant public health challenge among schoolchildren worldwide, emphasising the need for control efforts. The present study aimed to integrate epidemiological, morphometric, and mitochondrial genetic data on head lice across diverse regions of Pakistan.

Methods

A cross-sectional study of 1536 school-aged children was carried out across four districts in Pakistan. A structured questionnaire was used to collect data on risk factors. Head lice were morphologically examined and genomic DNA extracted. The mitochondrial markers cox 1 and cyt-b were amplified and sequenced.

Results

The overall prevalence of head lice infestation was 34%. Higher infestation rates were observed among females, children aged 6–10 years, those from large families, individuals with a history of previous infestations, and those from low socioeconomic backgrounds (p < 0.0001). Significant behavioral risk factors included sharing combs or towels and infrequent bathing (p < 0.0001). In contrast, protective factors included parental education, urban residence, and access to medical care (p < 0.0001. Morphometric analysis revealed clear sexual dimorphism, with females showing significantly greater total body length, post-antenna head width, pterothoracic width, and antennal length (p < 0.0001). Molecular analysis of cox1 and cyt-b genes showed that mitochondrial Clade C was predominant among the Pakistani isolates, indicating genetic links to South Asian and African lineages. One cyt-b sequence (PAK 1) grouped with Clade A, clustering with specimens from Thailand, Iraq, Germany, Laos, and Taiwan, suggesting a distinct lineage.

Conclusion

This study identifies Pakistan as a potential bridge in the global phylogeography of lice, reflecting both historical and ongoing interregional transmission. These findings contribute to evolutionary and parasitological research and offer a data-driven basis for developing school-based infestation control policies.

目的人头虫对全世界学龄儿童的公共卫生构成重大挑战，强调需要采取控制措施。本研究旨在整合巴基斯坦不同地区头虱的流行病学、形态计量学和线粒体遗传数据。方法对巴基斯坦四个地区1536名学龄儿童进行了横断面研究。采用结构化问卷收集风险因素数据。对头虱进行形态学检查，提取基因组DNA。扩增线粒体标记物cox 1和cyt-b并测序。结果全区头虱患病率为34%。在女性、6-10岁儿童、大家庭成员、有感染史的个体以及社会经济背景较低的人群中，感染率较高（p < 0.0001）。重要的行为危险因素包括共用梳子或毛巾以及不经常洗澡（p < 0.0001）。相比之下，保护性因素包括父母教育、城市居住和获得医疗保健（p < 0.0001）。形态计量学分析显示出明显的性别二态性，雌性的体长、天线后头宽、翼胸宽和天线长明显大于雌性（p < 0.0001）。cox1和cyt-b基因的分子分析显示，巴基斯坦分离株中线粒体进化支C占主导地位，表明与南亚和非洲谱系有遗传联系。一个cyt-b序列（PAK 1）与进化支A分群，与来自泰国、伊拉克、德国、老挝和台湾的标本聚类，提示不同的谱系。本研究确定巴基斯坦是虱子全球系统地理学的潜在桥梁，反映了历史上和正在进行的区域间传播。这些发现有助于进化和寄生虫学研究，并为制定以学校为基础的虫害控制政策提供数据驱动的基础。

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引用次数: 0

Morphological and Molecular Characterization of Hexostoma auxisi Palombi, 1943 (Polyopisthocotylea: Hexostomatidae) from Auxis rochei (Risso, 1810) (Teleostei: Scombridae) off Algerian Waters, Western Mediterranean 地中海西部阿尔及利亚海域长尾虾蛄（长尾虾蛄科）1943年的形态和分子特征(多子叶目：长尾虾蛄科

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01152-6

Zouhour El Mouna Ayadi, Meriem Amira Rebah, Delphine Gey, Fadila Tazerouti

Introduction

Hexostoma auxisi Palombi, 1943 was originally described by Palombi (1943) from Auxis thazard off Italy, Mediterranean Sea. The original description of this species lacks morphological and morpho-metrical data. In addition, no published sequence of this species is available in molecular engines.

Materials and Methods

Specimens of Hexostoma auxisi were collected from the gills of Auxis rochei (Risso, 1810) off the Algerian coast, Western Mediterranean. Monogenean were stained with acetic carmine, measured and drawn. Furthermore, molecular study was conducted using partial fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) of parasites and a tissue sample of the fish’s gills on which the monogeneans were found.

Results

In this study, we provide morpho-anatomical and morpho-metrical data of Hexostoma auxisi. Our specimens are morphologically close to those described by Palombi (1943) off Italy, Mediterranean Sea. The morphological study was supported by a molecular analysis of cox1 gene that reveals that our sequence of H. auxisi is distinct from its congener H. thynni by 20.51%.

Conlusion

The present study allowed us to confirm the taxonomic status of Hexostoma auxisi which belongs to the genus Hexostoma and the family Hexostomatidae.

hexostoma auxisi Palombi， 1943最初由Palombi（1943）描述，来自意大利地中海的Auxis thazard。该物种的原始描述缺乏形态学和形态测量数据。此外，在分子引擎中没有公开的该物种的序列。材料与方法从地中海西部阿尔及利亚海岸的罗氏螯虾（Risso, 1810）鳃中采集到鳃外异口（Hexostoma auxisi）。用乙酸胭脂红染色，测量并绘制单基因蛋白。此外，利用寄生虫线粒体细胞色素c氧化酶亚基1 （cox1）的部分片段和发现单基因虫的鱼鳃组织样本进行了分子研究。结果本研究提供了异构体异构体的形态学解剖和形态学测量数据。我们的标本在形态上与Palombi（1943）在意大利地中海外所描述的接近。形态学研究得到了cox1基因的分子分析的支持，结果表明，我们的H. auxisi序列与其同系物H. thynni序列差异20.51%。结论本研究确定了河口虫属河口虫科河口虫属的分类地位。

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引用次数: 0

“First of its Kind” in Brazilian Amazon: Spauligodon caxiuana n. sp. (Nematoda: Pharyngodonidae) Parasite of Thecadactylus rapicauda (Squamata: Phyllodactylidae), with an Updated Key to the Neotropical and Panamanian Species of the Genus 巴西亚马逊河流域“首见”：spuligodon caxiuana n. sp.（线虫纲：带刺线虫科）：rapicauda cadactylus（鳞状目：带刺线虫科）的寄生虫，附有该属新热带和巴拿马种的最新检索表

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01151-7

Jorge Kevin Silva Neves, Geovania Figueiredo da Silva, Ana Nunes Santos, Taynara Cristina Santos Tavares, Cynthya Elizabeth González, Gleomar Fabiano Maschio, Francisco Tiago de Vasconcelos Melo

Purpose

Describe a new species of Spauligodon Skrjabin, Schikhobalova, and Lagodovskaja, 1960, parasitizing Thecadactylus rapicauda (Houttuyn, 1782) in Brazilian Amazon.

Methods

A total of 40 host specimens were collected, anesthetized, and necropsied to search for helminths. The adult nematodes were collected, killed, and preserved in 70% ethanol. Specimens of both sexes were examined under a light microscope and a scanning electron microscope.

Results

The diagnosis of the new species was based on a set of characteristics: the number of caudal spines in both males and females, absence of a spicule, number and arrangement of cloacal papillae in males, egg shape, and vulva position in females. We provide an identification key to the species from the Neotropical and Panamanian realms. We also analyzed the egg morphology in the species description of Spauligodon and proposed a classification for egg morphology and shape.

Conclusions

Spauligodon caxiuana n. sp. is the 56th species of the genus, the fourth for the Neotropical realm, the third species record of Spauligodon spp. in Brazil, and the first in the Brazilian Amazon.

目的描述巴西亚马逊河流域一种寄生于快手爪蛾（cadactylus rapicauda, Houttuyn, 1782）的spuligodon Skrjabin， Schikhobalova, and Lagodovskaja, 1960)新种。方法采集宿主标本40份，麻醉后进行尸体解剖，寻找寄生虫。收集成虫，杀死，在70%乙醇中保存。在光学显微镜和扫描电子显微镜下对两性标本进行了检查。结果根据一组特征对该新种进行了诊断：雄性和雌性的尾刺数量、针状体缺失、雄性的阴囊乳头数量和排列、卵子形状和雌性的外阴位置。我们提供了一个识别关键的物种从新热带和巴拿马领域。我们还分析了spuligodon种属描述中的卵形态，提出了卵形态和形状的分类方法。结论spuligodon caxiuana n. sp.是spuligodon属的第56种，是新热带地区的第4种，是巴西spuligodon属的第3种记录，是巴西亚马逊地区的第1种记录。

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引用次数: 0

A New Species of Diaphorocleidus (Monopisthocotyla: Dactylogyridae), a Gill Parasite from Three Neotropical Characiform Fishes from Brazil 巴西三种新热带特征性鱼类的鳃寄生Diaphorocleidus一新种（单子叶目：Dactylogyridae）

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01165-1

Maurício Jorge, Mariana Bertholdi Ebert, Reinaldo José da Silva

Purpose

The members of the genus Diaphorocleidus (Monopisthocotyla: Dactylogyridae) infest the gills, nostrils, and body surface of characiform freshwater fishes from the Neotropical region. We characterize a new species of Diaphorocleidus from the gills of three acestrorhamphid species from the Pardo River (a sub-basin of the Paranapanema River basin) in the state of São Paulo, Brazil.

Methods

This study includes phylogenetic analyses of molecular data (partial 28 S rDNA), as well as morphological and morphometric assessments of parasites collected from the gills of 140 fishes (i.e., 50 A. lacustris, 80 P. bockmanni, and 10 P. fasciatus).

Results

These analyses indicate that Diaphorocleidus pindamirim n. sp. is closely related to D. neotropicalis but differs primarily in the morphology of the copulatory complex and molecular data. The small subunit of the accessory piece is shaped like a chela (or fishing hook tip), which is not observed in D. neotropicalis, where the small subunit is claw-shaped.

Conclusion

Integrating morphological and molecular evidence, we describe a new species of Diaphorocleidus and propose a general morphometric scheme for the genus. These findings underscore that combining morphology with molecular data is essential for robust species delimitation and will advance taxonomic and parasitological research in the Neotropical region.

目的Diaphorocleidus属的成员（单子叶目：Dactylogyridae）寄生于新热带地区的特征性淡水鱼的鳃、鼻孔和体表。我们从巴西圣保罗州Pardo河（Paranapanema河流域的一个子盆地）的三个acestrorhamphid物种的鳃中表征了一个新的Diaphorocleidus物种。方法对采集的140种鱼类（50种湖鲤、80种波克曼鲤和10种片尾鲤）鳃部寄生虫进行系统发育分析和形态计量学鉴定。结果这些分析表明，平达木裂尾蠓与新热带蠓有密切的亲缘关系，但主要在交配复合体的形态和分子数据上存在差异。附属片的小亚单位形状像螯（或鱼钩尖），这在D. neotropicalis中没有观察到，那里的小亚单位是爪形的。结论综合形态学和分子生物学的证据，我们描述了一个新种，并提出了该属的一般形态计量方案。这些发现强调了将形态学与分子数据结合起来对于建立强有力的物种划界至关重要，并将推动新热带地区的分类学和寄生虫学研究。

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引用次数: 0

Babesia spp. in Domestic Animals from Rural Areas of Cauca Department: Previous Exposure and Molecular Detection Among Canines, Bovines and Equines 考卡省农村地区家畜中的巴贝斯虫：犬、牛和马的既往暴露和分子检测

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01156-2

Carlos Ramiro Silva-Ramos, Juan Andrés Niño Rodríguez, Juliana Gil-Mora, Paola Betancourt-Ruiz, Heidy- C. Martínez-Díaz, Elkin Forero-Becerra, J. Manuel Matiz-González, Eliana Bolaños, Luz-Adriana Olaya-M, Efraín Benavides, Marylin Hidalgo

Purpose

Babesia species are tick-borne protozoan parasites which affect several animal species. Babesia spp. infections are significantly important for veterinary medicine, affecting a wide range of domestic animal species such as dogs, cattle, and horses. In Colombia, studies of Babesia spp. infections in domestic animals are scarce. Thus, the aim of the present study was to explore the circulation of these parasites among domestic canines, bovines and equines from the department of Cauca.

Methods

Between August and November, 2017, active domestic animal sampling of cattle was performed in eight rural areas of four municipalities of Cauca department. Serum and whole-blood samples were obtained from all specimens for serological and molecular tests. Immunofluorescence assays were performed on all serum samples to detect antibodies against Babesia spp., and DNA was extracted from whole-blood samples to perform a genus-specific PCR to identify the presence of Babesia spp.

Results

A total of 198 samples were collected: 52.5% from dogs, 32.3% from horses, and 15.2% from cattle. Seroprevalence rates showed that 58.1% of domestic animals were exposed to Babesia spp., with the highest rates among equines (65.6%). Molecular detection revealed that 17.7% had an active Babesia spp. infection, being more frequent among cattle (53.3%). Phylogenetic analysis indicated that Babesia bigemina was the primary species identified.

Conclusion

This study provides critical data on Babesia infections in domestic animals in Cauca department, revealing active infections and previous exposures among domestic animals from the region.

目的巴贝虫是蜱传原生动物寄生虫，影响多种动物。巴贝斯虫感染对兽医非常重要，影响广泛的家畜物种，如狗、牛和马。在哥伦比亚，关于家畜感染巴贝斯虫的研究很少。因此，本研究的目的是探讨这些寄生虫在考卡省家畜、牛和马之间的传播。方法2017年8 - 11月，在考卡省4个市的8个农村地区对牛进行家畜活动抽样。从所有标本中提取血清和全血样本进行血清学和分子检测。结果共采集198份样本，其中犬类52.5%，马类32.3%，牛类15.2%。结果显示，58.1%的家畜暴露于巴贝斯虫，其中以马的感染率最高（65.6%）。分子检测结果显示，17.7%的人感染巴贝斯虫，其中以牛多见（53.3%）。系统发育分析表明，该虫为主要种。结论本研究提供了考卡地区家畜巴贝斯虫感染情况的关键数据，揭示了该地区家畜的活动性感染和既往暴露情况。

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引用次数: 0

A New Neotropical Species of Gnathostoma (Nematoda: Gnathostomatidae) from the Northern Four-Eyed Opossum Philander vossi (Marsupialia) 北方四眼负鼠（有袋目）颌口虫一新种（线虫纲：颌口虫科）

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica

Pub Date : 2025-11-14 DOI: 10.1007/s11686-025-01155-3

M. Á. Mosqueda-Cabrera, E. Sánchez-Miranda, L. D. Castillo-Loeza, G. Torres-Carrera, L. García-Prieto

Purpose

This work provides a detailed morphological description of a previously identified but unnamed lineage inside Gnathostoma spp. from Mexico.

Methods

Specimens of the four-eyed opossum Philader vossi were collected in Tlacotalpan, Veracruz, Mexico. Specimens were examined morphologically using both light microscopy and scanning electron microscopy. Additionally, we generate DNA sequences for the following loci: mitochondrial cox1 and nuclear 5.8S rRNA and ITS-2 and 28S rDNA.

Results

The new species, Gnathostoma mexicanum n. sp., differs from G. turgidum Stossich, 1902, the common gnathostomid species infecting Didelphis spp. from the Americas, in its smaller body size, fewer teeth on the cuticular spines at anterior half of body, as well as site of infection (pyloric region vs. stomach layers). Host specificity further distinguishes the two species. Additionally, molecular data show that the new species clearly diverges from its congeners.

Conclusions

This work represents the fourth Gnathostoma species described for Mexican mammals and the eight recorded in the Americas.

目的：本研究对墨西哥Gnathostoma spp.中一个先前鉴定但未命名的谱系进行了详细的形态学描述。方法在墨西哥韦拉克鲁斯州特拉科塔尔潘采集四眼负鼠标本。使用光学显微镜和扫描电子显微镜对标本进行形态学检查。此外，我们还生成了以下位点的DNA序列：线粒体cox1和核5.8S rRNA以及ITS-2和28S rDNA。结果该新种为墨西哥齿颌虫，不同于美洲Didelphis常见的齿颌虫种G. turgidum Stossich, 1902，其体型较小，身体前半部分表皮棘上的牙齿较少，感染部位（幽门区与胃层）也较少。宿主特异性进一步区分了这两个物种。此外，分子数据表明，新物种明显不同于其同系物。结论本研究是墨西哥哺乳动物中发现的第4种颌口，是美洲发现的8种颌口。

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