Pub Date : 2010-12-24DOI: 10.3724/SP.J.1035.2010.01198
W. Juan, X. Dai, Song Zengfu, Ying-jie Pan, Z. Qinghua
{"title":"ISOLATION AND IDENTIFICATION OF AMMONIFYING BACTERIUM AND CHARACTERISTICS OF DEGRADING NH 3 -N: ISOLATION AND IDENTIFICATION OF AMMONIFYING BACTERIUM AND CHARACTERISTICS OF DEGRADING NH 3 -N","authors":"W. Juan, X. Dai, Song Zengfu, Ying-jie Pan, Z. Qinghua","doi":"10.3724/SP.J.1035.2010.01198","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2010.01198","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70057094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-24DOI: 10.3724/SP.J.1035.2010.01172
Wei-Chuan Jiang, Huanzhang Liu, Z. Duan, W. Cao
{"title":"STUDIES ON THE DRIFT PATTERN OF DRIFTING FISH EGGS WITH TRACERS IN UPPER YANGTZE RIVER: STUDIES ON THE DRIFT PATTERN OF DRIFTING FISH EGGS WITH TRACERS IN UPPER YANGTZE RIVER","authors":"Wei-Chuan Jiang, Huanzhang Liu, Z. Duan, W. Cao","doi":"10.3724/SP.J.1035.2010.01172","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2010.01172","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70057309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-24DOI: 10.3724/SP.J.1035.2010.01190
N. Zhu, Q. Gao, Wenxing Li, Ying Song, W. Yao, P. Nie
{"title":"OBSERVATION ON THREE SPECIES OF SINERGASILUS (COPEPODA: ERGASILIDAE) BY SCANNING ELECTRON MICROSCOPY: OBSERVATION ON THREE SPECIES OF SINERGASILUS (COPEPODA: ERGASILIDAE) BY SCANNING ELECTRON MICROSCOPY","authors":"N. Zhu, Q. Gao, Wenxing Li, Ying Song, W. Yao, P. Nie","doi":"10.3724/SP.J.1035.2010.01190","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2010.01190","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70057424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-24DOI: 10.3724/SP.J.1035.2010.01130
W. Rui, Xiaoyu Qing, Gui Jian-fang
Fructose-1,6-bisphosphatase is one of the key rate-limiting enzymes in gluconeogenesis,which plays important roles in carbohydrate metabolism.Mammals have two isoforms,liver and muscle fructose-1,6-bisphosphatase encoded by Fbp1 and Fbp2 respectively.Gibel carp is widely cultured as an economic fish in China.However,the fructose-1,6-bisphosphatase gene has not been elucidated in teleosts,especially its tissue distribution in adult fish and spatiotemporal expression in embryogenesis.In this study,we cloned the full-length cDNA of gibel carp Fbp1 by RACE polymerase chain reaction from the gastrula embryo SMART cDNA library,and we also examined its expression pattern in the tissues of adult fish and the developmental process of embryos in this fish by gene specific primers.The full length sequence of CagFbp1 consists of 1170 base pairs which encodes 337 amino acids.Multiple alignment and phylogenetic analysis showed that the cloned gene was liver Fbp of gibel carp.The tissue expression pattern analysis by RT-PCR with specific primers showed that CagFbp1 was expressed in the liver,brain,heart,spleen,kidney,intestine,muscle and ovary,and the expression in the liver was obviously higher than others.At the same times,there were two protein bands in liver by western blot analysis,one was common in the detected tissue except muscle,and the other was specific for liver.However,only one band emerged in the muscle,which was specific for muscle tissues.Mature eggs and ontogenic analysis by RT-PCR and western blotting with specific primers showed that both the transcripts and proteins of CagFbp1 were maternal.The transcripts were increased from gastrula and reached a higher level in neurula till hatching.Interestingly,there was a new band with smaller molecular weight other than the maternal proteins after tail bud stage,which was similar to the liver specific band.These results indicated that the fructose-1,6-bisphosphatase gene cloned in gibel carp was liver isoform,and there might be at least two isoenzymes,the liver fructose-1,6-bisphosphatase and muscle one in teleost.
{"title":"MOLECULAR CLONING AND EXPRESSION ANALYSIS OF FRUCTOSE-1,6-BISPHOSPHATASE IN GIBEL CARP","authors":"W. Rui, Xiaoyu Qing, Gui Jian-fang","doi":"10.3724/SP.J.1035.2010.01130","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2010.01130","url":null,"abstract":"Fructose-1,6-bisphosphatase is one of the key rate-limiting enzymes in gluconeogenesis,which plays important roles in carbohydrate metabolism.Mammals have two isoforms,liver and muscle fructose-1,6-bisphosphatase encoded by Fbp1 and Fbp2 respectively.Gibel carp is widely cultured as an economic fish in China.However,the fructose-1,6-bisphosphatase gene has not been elucidated in teleosts,especially its tissue distribution in adult fish and spatiotemporal expression in embryogenesis.In this study,we cloned the full-length cDNA of gibel carp Fbp1 by RACE polymerase chain reaction from the gastrula embryo SMART cDNA library,and we also examined its expression pattern in the tissues of adult fish and the developmental process of embryos in this fish by gene specific primers.The full length sequence of CagFbp1 consists of 1170 base pairs which encodes 337 amino acids.Multiple alignment and phylogenetic analysis showed that the cloned gene was liver Fbp of gibel carp.The tissue expression pattern analysis by RT-PCR with specific primers showed that CagFbp1 was expressed in the liver,brain,heart,spleen,kidney,intestine,muscle and ovary,and the expression in the liver was obviously higher than others.At the same times,there were two protein bands in liver by western blot analysis,one was common in the detected tissue except muscle,and the other was specific for liver.However,only one band emerged in the muscle,which was specific for muscle tissues.Mature eggs and ontogenic analysis by RT-PCR and western blotting with specific primers showed that both the transcripts and proteins of CagFbp1 were maternal.The transcripts were increased from gastrula and reached a higher level in neurula till hatching.Interestingly,there was a new band with smaller molecular weight other than the maternal proteins after tail bud stage,which was similar to the liver specific band.These results indicated that the fructose-1,6-bisphosphatase gene cloned in gibel carp was liver isoform,and there might be at least two isoenzymes,the liver fructose-1,6-bisphosphatase and muscle one in teleost.","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70056888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-24DOI: 10.3724/SP.J.1035.2010.01228
Min Xu, Xu-Dong Xu
{"title":"CORRELATION BETWEEN SIZES OF GAS VESICLES AND GVPA/GVPC IN MICROCYSTIS : CORRELATION BETWEEN SIZES OF GAS VESICLES AND GVPA/GVPC IN MICROCYSTIS","authors":"Min Xu, Xu-Dong Xu","doi":"10.3724/SP.J.1035.2010.01228","DOIUrl":"https://doi.org/10.3724/SP.J.1035.2010.01228","url":null,"abstract":"","PeriodicalId":6937,"journal":{"name":"水生生物学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70057277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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