C. V. Sklyarenko, V. Sorokovikov, Z. Koshkareva, A. Zhivotenko, B. Damdinov
{"title":"Conservative Treatment of Cervicobrachial Syndrome in Patients with Cervical Osteochondrosis (Literature Review)","authors":"C. V. Sklyarenko, V. Sorokovikov, Z. Koshkareva, A. Zhivotenko, B. Damdinov","doi":"10.29413/abs.2019-4.6.5","DOIUrl":"https://doi.org/10.29413/abs.2019-4.6.5","url":null,"abstract":"","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"115 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76794022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Potapov, Z. Koshkareva, A. Zhivotenko, A. Gorbunov, V. Sorokovikov
{"title":"The Experience of Using the Implantable Interspinous System COFLEX in the Surgical Treatment of Compression Forms of Lumbosacral Radiculitis","authors":"V. Potapov, Z. Koshkareva, A. Zhivotenko, A. Gorbunov, V. Sorokovikov","doi":"10.29413/abs.2019-4.6.8","DOIUrl":"https://doi.org/10.29413/abs.2019-4.6.8","url":null,"abstract":"","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"22 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87063278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationship of Immune Status Indicators and Hormonal Profile in Patients with Coxarthrosis","authors":"L. A. Dmitrieva, Y. Pivovarov, V. F. Lebedev","doi":"10.29413/abs.2019-4.6.2","DOIUrl":"https://doi.org/10.29413/abs.2019-4.6.2","url":null,"abstract":"","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80520598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-14DOI: 10.29413/abs.2019-4.5.15
O. Bolshedvorskaya, K. Protasov, Y. Batoroev, P. S. Ulybin, V. Dvornichenko
Background. Perioperative myocardial ischemia often complicates extracardiac surgery. The problems of its prediction, diagnostics, treatment and prevention are not solved. Frequency, structure and clinical features of cardiac ischemic complications of surgical treatment of lung cancer are not well understood.The aim of the study was to investigate the frequency of postoperative myocardial infarction and myocardial ischemia in the surgical treatment of patients with non-small cell lung cancer.Methods. The frequency (%) of myocardial infarction (MI) with and without ST segment elevation of electrocardiogram, acute myocardial ischemia in a complete sample of cancer patients (n = 2051) who underwent treatment for non-small cell lung cancer for the last 10 years was calculated. By comparing the relative indicators and calculating the Odds Ratio (OR), we studied the relationship between the cardiac ischemic events with age, type of surgery, prevalence and localization of the tumour. The clinical manifestations and pathomorphology of postoperative myocardial infarction were analysed.Results. Cardiac ischemic complications of thoracotomy for lung cancer occurred in 2.73 % (95% CI 1.98–3.48) cases. Myocardial infarction with ST segment elevation was recorded in 1.07 % (95% CI 0.58–1.57) patients, MI without ST segment elevation – in 0.54 % (95% CI 0.17–0.9), myocardial ischemia – in 1.12 % (95% CI 0.62–1.63). An increase in the frequency of ischemic events after pneumonectomy was observed compared with lobectomy (OR 6.5, 95% CI 3.5–12.2) and after right-sided pneumonectomy compared with left-sided one (OR 3.2, 95% CI 1.6–6.3), and also the age over 70. Hospital mortality from MI was 39.3 %. According to autopsies of patients who died of МI, coronary atherothrombosis was detected in 2 of 22 cases.Conclusion. In the surgical treatment of non-small cell lung cancer, cardiac ischemic events develop in 2.73 % of patients. The greatest risk is associated with right-sided pneumonectomy.
背景。围手术期心肌缺血常使心外手术复杂化。其预测、诊断、治疗和预防等问题尚未得到解决。肺癌手术治疗心脏缺血性并发症的发生频率、结构及临床特点尚不清楚。本研究旨在探讨非小细胞肺癌手术治疗后心肌梗死和心肌缺血的发生率。计算了过去10年接受非小细胞肺癌治疗的癌症患者(n = 2051)的心肌梗死(MI)频率(%)，包括心电图ST段抬高和急性心肌缺血。通过比较相关指标和计算比值比(Odds Ratio, OR)，研究心脏缺血事件与年龄、手术类型、肿瘤患病率和肿瘤定位的关系。分析了术后心肌梗死的临床表现和病理形态。肺癌开胸术后心脏缺血性并发症发生率为2.73% (95% CI 1.98 ~ 3.48)。ST段抬高的心肌梗死发生率为1.07% (95% CI 0.58-1.57)，无ST段抬高的心肌梗死发生率为0.54% (95% CI 0.17-0.9)，心肌缺血发生率为1.12% (95% CI 0.62-1.63)。与肺叶切除术相比，肺切除术后缺血性事件发生频率增加(OR 6.5, 95% CI 3.5-12.2);与左侧肺切除术相比，右侧肺切除术后缺血性事件发生频率增加(OR 3.2, 95% CI 1.6-6.3);年龄在70岁以上的患者也增加。心肌梗死的住院死亡率为39.3%。根据对死于МI的22例患者的尸检，发现2例冠状动脉粥样硬化形成。在非小细胞肺癌的手术治疗中，2.73%的患者发生心脏缺血事件。风险最大的是右侧全肺切除术。
{"title":"Postoperative Cardiac Ischemic Complications in Lung Cancer Patients","authors":"O. Bolshedvorskaya, K. Protasov, Y. Batoroev, P. S. Ulybin, V. Dvornichenko","doi":"10.29413/abs.2019-4.5.15","DOIUrl":"https://doi.org/10.29413/abs.2019-4.5.15","url":null,"abstract":"Background. Perioperative myocardial ischemia often complicates extracardiac surgery. The problems of its prediction, diagnostics, treatment and prevention are not solved. Frequency, structure and clinical features of cardiac ischemic complications of surgical treatment of lung cancer are not well understood.The aim of the study was to investigate the frequency of postoperative myocardial infarction and myocardial ischemia in the surgical treatment of patients with non-small cell lung cancer.Methods. The frequency (%) of myocardial infarction (MI) with and without ST segment elevation of electrocardiogram, acute myocardial ischemia in a complete sample of cancer patients (n = 2051) who underwent treatment for non-small cell lung cancer for the last 10 years was calculated. By comparing the relative indicators and calculating the Odds Ratio (OR), we studied the relationship between the cardiac ischemic events with age, type of surgery, prevalence and localization of the tumour. The clinical manifestations and pathomorphology of postoperative myocardial infarction were analysed.Results. Cardiac ischemic complications of thoracotomy for lung cancer occurred in 2.73 % (95% CI 1.98–3.48) cases. Myocardial infarction with ST segment elevation was recorded in 1.07 % (95% CI 0.58–1.57) patients, MI without ST segment elevation – in 0.54 % (95% CI 0.17–0.9), myocardial ischemia – in 1.12 % (95% CI 0.62–1.63). An increase in the frequency of ischemic events after pneumonectomy was observed compared with lobectomy (OR 6.5, 95% CI 3.5–12.2) and after right-sided pneumonectomy compared with left-sided one (OR 3.2, 95% CI 1.6–6.3), and also the age over 70. Hospital mortality from MI was 39.3 %. According to autopsies of patients who died of МI, coronary atherothrombosis was detected in 2 of 22 cases.Conclusion. In the surgical treatment of non-small cell lung cancer, cardiac ischemic events develop in 2.73 % of patients. The greatest risk is associated with right-sided pneumonectomy.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84540709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-14DOI: 10.29413/abs.2019-4.5.13
M. I. Mikhailichenko, K. Shapovalov, V. A. Mudrov, O. Gruzdeva
The relevance of cold injury is due to its high specific weight in the structure of injuries, complexities of complex therapy, and unsatisfactory results of treatment. It is known that in the pathogenesis of local cold injury, the leading role belongs to endothelial dysfunction, which secretes a huge amount of biologically active substances, including matrix metalloproteinases.Aim: to determine the dynamics of the content of matrix metalloproteinases of the second subfamily (MMP-2, MMP-9) in the serum of patients with local cold injury.Materials and methods. The study included 80 patients (60 men, 20 women) with frostbite of the III–IV degree of the foot to the level of the lower third of the leg in the late reactive period and the period of granulation and epithelization.Results. In the late reactive period in patients with cryopreservation, the level of MMP-2 was 3.4 times higher relative to the control (p = 0.011), on day 30 MMP-2 values did not differ from the control parameters (p = 0.103). The level of collagenase B (MMP-9) in patients with local cold injury on day 5 was 14.5 times higher than the control parameters (p = 0.002), on day 30 – 12.5 times higher compared to the control group (p = 0.000094).Conclusion. During the analysis of the data obtained, we can think about the dual nature of collagenases during the processes of inflammation. In a situation of distress, collagenases prevent endothelial dysfunction by eliminating the cellular structures of the endothelium to ensure adequate metabolism in tissues. Their importance as markers of failure of adequate flow of reparative processes is not excluded.
{"title":"Dynamics of Matrix Metalloproteinases in Local Cold Injury","authors":"M. I. Mikhailichenko, K. Shapovalov, V. A. Mudrov, O. Gruzdeva","doi":"10.29413/abs.2019-4.5.13","DOIUrl":"https://doi.org/10.29413/abs.2019-4.5.13","url":null,"abstract":"The relevance of cold injury is due to its high specific weight in the structure of injuries, complexities of complex therapy, and unsatisfactory results of treatment. It is known that in the pathogenesis of local cold injury, the leading role belongs to endothelial dysfunction, which secretes a huge amount of biologically active substances, including matrix metalloproteinases.Aim: to determine the dynamics of the content of matrix metalloproteinases of the second subfamily (MMP-2, MMP-9) in the serum of patients with local cold injury.Materials and methods. The study included 80 patients (60 men, 20 women) with frostbite of the III–IV degree of the foot to the level of the lower third of the leg in the late reactive period and the period of granulation and epithelization.Results. In the late reactive period in patients with cryopreservation, the level of MMP-2 was 3.4 times higher relative to the control (p = 0.011), on day 30 MMP-2 values did not differ from the control parameters (p = 0.103). The level of collagenase B (MMP-9) in patients with local cold injury on day 5 was 14.5 times higher than the control parameters (p = 0.002), on day 30 – 12.5 times higher compared to the control group (p = 0.000094).Conclusion. During the analysis of the data obtained, we can think about the dual nature of collagenases during the processes of inflammation. In a situation of distress, collagenases prevent endothelial dysfunction by eliminating the cellular structures of the endothelium to ensure adequate metabolism in tissues. Their importance as markers of failure of adequate flow of reparative processes is not excluded.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80093290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background. Management of the reparative process is an urgent task of modern medicine. In our opinion, the development of pathogenetically grounded approaches to optimizing the repair process for managing the interrelations of stromal cells is promising. One of the promising areas in this regard is the impact on the MAPK-cascades.Aim: to study the expression of MAP-kinase mechanisms in the regulation of repair by the example of a musculocutaneous wound.Methods. A linear muscular skin wound was modeled using Wistar rats weighing 220–250 g at the age of 9 months (n = 24). Immunofluorescence staining was performed to detect the activity of p38, JNK, and ERK MAPK cascades from 1 to 30 days.Results. It was established that specific staining in the area of connective tissue formation during staining with p38 MAPK and its phosphorylated form was first observed on the 3rd day, and its maximum severity occurred at the same time. On the 7th and 14th day, small zones in the area of scar formation were minimally stained. The phosphorylated part of the JNK-cascade in the zone of traumatic injury was detected starting from the 1st day after the injury. Bright color persisted on the 3rd day. On the 7th day, the color was minimal, and by the 14th day a second wave of expression was observed. ERK-staining was observed from the 1st to the 14th day with a peak activity on the 3rd day.Conclusion. Thus, we revealed the simultaneous involvement of p38, JNK-, and ERK-cascades in the regulation of the reparative process in the conditions of a musculoskeletal wound. At the same time, it is noteworthy that the peak activity of all cascades coincides and falls on the 3rd day.
{"title":"Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds","authors":"I. Shurygina, V. A. Umanets, M. Shurygin","doi":"10.29413/abs.2019-4.5.9","DOIUrl":"https://doi.org/10.29413/abs.2019-4.5.9","url":null,"abstract":"Background. Management of the reparative process is an urgent task of modern medicine. In our opinion, the development of pathogenetically grounded approaches to optimizing the repair process for managing the interrelations of stromal cells is promising. One of the promising areas in this regard is the impact on the MAPK-cascades.Aim: to study the expression of MAP-kinase mechanisms in the regulation of repair by the example of a musculocutaneous wound.Methods. A linear muscular skin wound was modeled using Wistar rats weighing 220–250 g at the age of 9 months (n = 24). Immunofluorescence staining was performed to detect the activity of p38, JNK, and ERK MAPK cascades from 1 to 30 days.Results. It was established that specific staining in the area of connective tissue formation during staining with p38 MAPK and its phosphorylated form was first observed on the 3rd day, and its maximum severity occurred at the same time. On the 7th and 14th day, small zones in the area of scar formation were minimally stained. The phosphorylated part of the JNK-cascade in the zone of traumatic injury was detected starting from the 1st day after the injury. Bright color persisted on the 3rd day. On the 7th day, the color was minimal, and by the 14th day a second wave of expression was observed. ERK-staining was observed from the 1st to the 14th day with a peak activity on the 3rd day.Conclusion. Thus, we revealed the simultaneous involvement of p38, JNK-, and ERK-cascades in the regulation of the reparative process in the conditions of a musculoskeletal wound. At the same time, it is noteworthy that the peak activity of all cascades coincides and falls on the 3rd day.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80781916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the research was to analyze the effectiveness of empirical therapy in patients with established complicated upper urinary tract infection (complicated pyelonephritis).Materials and methods. 51 patients who were treated in 2018 in the urological department of the Irkutsk City Clinical Hospital N 1 were subjected to a retrospective study. The average age of patients was 55.9 ± 16.9 years.Results. Successful empirical therapy was considered in the absence of correction of the treatment regimen and the normalization of clinical blood and urine tests. Of the patients included in the study, successful empirical therapy was found in 24 (47%) patients, and required correction in 27 (52.9 %) patients. Cephalosporins as monotherapy were ineffective in 24 (57.1 %) patients, carbopenems – in one (25 %), fluoroquinolones – in all (100 %). Aminoglycosides showed the best result as a combination therapy with cephalosporins – 100 % (n = 3) of success. Based on multivariate logistic regression analysis, it was established that the detection of microhematuria and uremia in clinical analyzes upon admission to the hospital increases the likelihood of empirical monotherapy with third-generation cephalosporins by 20 % and 27 %, respectively.Conclusion. It is advisable to refrain from the use of monotherapy with fluoroquinolones and third-generation cephalosporins until more information is available about the resistance of uropathogens in the population of the Irkutsk region. Patients with impaired renal function require particularly close attention of the clinician when choosing a scheme for the empirical treatment of complicated pyelonephritis.
{"title":"Empirical Therapy of Complicated Pyelonephritis","authors":"V. Beloborodov, V. Vorobev, P. V. Opanasyuk","doi":"10.29413/abs.2019-4.5.4","DOIUrl":"https://doi.org/10.29413/abs.2019-4.5.4","url":null,"abstract":"The aim of the research was to analyze the effectiveness of empirical therapy in patients with established complicated upper urinary tract infection (complicated pyelonephritis).Materials and methods. 51 patients who were treated in 2018 in the urological department of the Irkutsk City Clinical Hospital N 1 were subjected to a retrospective study. The average age of patients was 55.9 ± 16.9 years.Results. Successful empirical therapy was considered in the absence of correction of the treatment regimen and the normalization of clinical blood and urine tests. Of the patients included in the study, successful empirical therapy was found in 24 (47%) patients, and required correction in 27 (52.9 %) patients. Cephalosporins as monotherapy were ineffective in 24 (57.1 %) patients, carbopenems – in one (25 %), fluoroquinolones – in all (100 %). Aminoglycosides showed the best result as a combination therapy with cephalosporins – 100 % (n = 3) of success. Based on multivariate logistic regression analysis, it was established that the detection of microhematuria and uremia in clinical analyzes upon admission to the hospital increases the likelihood of empirical monotherapy with third-generation cephalosporins by 20 % and 27 %, respectively.Conclusion. It is advisable to refrain from the use of monotherapy with fluoroquinolones and third-generation cephalosporins until more information is available about the resistance of uropathogens in the population of the Irkutsk region. Patients with impaired renal function require particularly close attention of the clinician when choosing a scheme for the empirical treatment of complicated pyelonephritis.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84291374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
U. Izbanova, L. Lukhnova, T. Meka-Mechenko, E. B. Sansyzbaev, A. Kairzhanova, V. B. Shvedyuk, E. Begimbayeva, V. Yu. Sushchykh, A. Shevtsov
Today, anthrax is recorded in many countries around the world, in Kazakhstan – in the form of sporadic cases or small outbreaks. Despite the endemicity of anthrax in Kazakhstan, the genetic diversity strains is not well described. At the moment, MLVA-25, which is sufficient for molecular and epidemiological monitoring, is recognized as one of the most discriminatory methods of genotyping.Objective: to determine the cultural and morphological properties, the genomic characteristics of the strains of the anthrax pathogen, their geographical distribution in the territory of Kazakhstan during the outbreaks in 2016, a comparative analysis with collection strains isolated since 1962.Methods: microbiological, genetic research methods were used in the work.Results. We investigated 11 strains of B. anthracis, which were isolated in 2016 in Kazakhstan. For comparison of genotypes, 26 strains were used from the collection of pathogenic microorganisms of the A.M. Aykimbaev’s Kazakh Scientific Center for Quarantine and Zoonotic Diseases. Phylogenetic analysis clustered 37 strains of B. anthracis into three clusters and 23 genotypes.The results of studying the phenotypic properties of anthrax strains by the main identification tests showed that all the strains studied in the experiment and isolated from 1961 to 2016 had biological properties characteristic of typical of B. anthracis strains.The anthrax strains isolated in 2016 are characterized as significant variability, as well as the circulation of the same genotypes and clusters in different areas of Kazakhstan. MLVA-profiles of analyzed Kazakhstan strains are unique and do not fully coincide with any studied strain from MLVAbank. On the MST-tree, Kazakhstan’s strains are located in three clusters, as on the phylogenetic tree.Conclusion: Molecular genetic analysis of B. anthracis strains enhances the ability of epidemiologists to track the sources and pathways of infection.It is necessary to improve the tracking system for strains of especially dangerous infections in Kazakhstan using modern molecular genetic methods.
{"title":"Biological Properties and Molecular Genetic Characteristics of Bacillus Anthracis Strains Isolated During Anthrax Outbreaks in Kazakhstan in 2016","authors":"U. Izbanova, L. Lukhnova, T. Meka-Mechenko, E. B. Sansyzbaev, A. Kairzhanova, V. B. Shvedyuk, E. Begimbayeva, V. Yu. Sushchykh, A. Shevtsov","doi":"10.29413/abs.2019-4.5.7","DOIUrl":"https://doi.org/10.29413/abs.2019-4.5.7","url":null,"abstract":"Today, anthrax is recorded in many countries around the world, in Kazakhstan – in the form of sporadic cases or small outbreaks. Despite the endemicity of anthrax in Kazakhstan, the genetic diversity strains is not well described. At the moment, MLVA-25, which is sufficient for molecular and epidemiological monitoring, is recognized as one of the most discriminatory methods of genotyping.Objective: to determine the cultural and morphological properties, the genomic characteristics of the strains of the anthrax pathogen, their geographical distribution in the territory of Kazakhstan during the outbreaks in 2016, a comparative analysis with collection strains isolated since 1962.Methods: microbiological, genetic research methods were used in the work.Results. We investigated 11 strains of B. anthracis, which were isolated in 2016 in Kazakhstan. For comparison of genotypes, 26 strains were used from the collection of pathogenic microorganisms of the A.M. Aykimbaev’s Kazakh Scientific Center for Quarantine and Zoonotic Diseases. Phylogenetic analysis clustered 37 strains of B. anthracis into three clusters and 23 genotypes.The results of studying the phenotypic properties of anthrax strains by the main identification tests showed that all the strains studied in the experiment and isolated from 1961 to 2016 had biological properties characteristic of typical of B. anthracis strains.The anthrax strains isolated in 2016 are characterized as significant variability, as well as the circulation of the same genotypes and clusters in different areas of Kazakhstan. MLVA-profiles of analyzed Kazakhstan strains are unique and do not fully coincide with any studied strain from MLVAbank. On the MST-tree, Kazakhstan’s strains are located in three clusters, as on the phylogenetic tree.Conclusion: Molecular genetic analysis of B. anthracis strains enhances the ability of epidemiologists to track the sources and pathways of infection.It is necessary to improve the tracking system for strains of especially dangerous infections in Kazakhstan using modern molecular genetic methods.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79966641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-14DOI: 10.29413/abs.2019-4.5.22
S. Nurtazin, A. Shevtsov, V. B. Lutsa, E. Ramankulov, Z. Sayakova, A. A. Abdrasilova, A. Zhunusova, N. P. Kabysheva, A. Rysbekova, V. Sadovskaya, A. B. Eszhanov, I. Utepova, A. Berdibekov, M. Kulemin, Zh. U. Katuova, B. Atshabar
Revealed by morphological characters, physiological status, and genetic diversity of populations of the main plague host Rhombomys opimus Licht., 1823 in Central Asia desert natural focus is described.Differences in the skull parameters of R. opimus from different populations were revealed. It’s shown that gerbils from Moyunkum are separate autonomous populations group. Samples from Moyunkum and Mangyshlak differed from other samples. In Balkhash-Alakol depression found two regional complexes: Pre-Balkhash and Dzungarian.Study results of free amino acids level in R. opimus blood serum obtained by gas-liquid chromatography of blood sera from animals captured in different zones of Central Asian focus gave statistically significant differences. To determine genetic variability in ecological and geographical isolation of R. opimus populations the DNA was genotyped.DNA samples analysis combined the studied gerbil from the desert focus into four clusters with eighteen haplotypes. R. opimus sequence analysis taking into account data from territories of Iran, Kazakhstan and China, clustered into three large clusters. First cluster combined the sequences of Kazakhstan and China samples, while great gerbil captured in Kazakhstan is located in a separate treasure. Second and third clusters include sequences of a great gerbil captured in Iran.
{"title":"Morphological, Physiological and Genetic Characteristics of Populations of the Main Plague Host Rhombomys opimus Licht., 1823 in the Central Asian Desert Natural Focus of Plague","authors":"S. Nurtazin, A. Shevtsov, V. B. Lutsa, E. Ramankulov, Z. Sayakova, A. A. Abdrasilova, A. Zhunusova, N. P. Kabysheva, A. Rysbekova, V. Sadovskaya, A. B. Eszhanov, I. Utepova, A. Berdibekov, M. Kulemin, Zh. U. Katuova, B. Atshabar","doi":"10.29413/abs.2019-4.5.22","DOIUrl":"https://doi.org/10.29413/abs.2019-4.5.22","url":null,"abstract":"Revealed by morphological characters, physiological status, and genetic diversity of populations of the main plague host Rhombomys opimus Licht., 1823 in Central Asia desert natural focus is described.Differences in the skull parameters of R. opimus from different populations were revealed. It’s shown that gerbils from Moyunkum are separate autonomous populations group. Samples from Moyunkum and Mangyshlak differed from other samples. In Balkhash-Alakol depression found two regional complexes: Pre-Balkhash and Dzungarian.Study results of free amino acids level in R. opimus blood serum obtained by gas-liquid chromatography of blood sera from animals captured in different zones of Central Asian focus gave statistically significant differences. To determine genetic variability in ecological and geographical isolation of R. opimus populations the DNA was genotyped.DNA samples analysis combined the studied gerbil from the desert focus into four clusters with eighteen haplotypes. R. opimus sequence analysis taking into account data from territories of Iran, Kazakhstan and China, clustered into three large clusters. First cluster combined the sequences of Kazakhstan and China samples, while great gerbil captured in Kazakhstan is located in a separate treasure. Second and third clusters include sequences of a great gerbil captured in Iran.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85075522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. V. Moroz, A. Karateev, E. Kryukov, A. Sokolov, E. Artemkin
Intaking antithrombotic funds (ATA) and non-steroidal anti-inflammatory drugs (NSAIDs) is one of the most frequent causes of pathology in gastrointestinal (GI) tract.The purpose of the study: comparison of pathological changes of the mucous membrane in the upper GI tract, that occur against the background of ATA and NSAIDs admission.Material and methods. Endoscopic data of two groups of patients taking ATA and NSAIDS have been compared. The first group of 448 patients from the 10th Gastrointestinal Department in N.N. Burdenko Main Military Clinical Hospital was on record from 2013 to 2017. The patients had erosive ulcerous changes of gastrointestinal mucosa, occurred against the background of the ATA admission. The second group comprised 6431 patients with rheumatic diseases. They were hospitalized in the clinic of V.A. Nasonova Research Institute of Rheumatology in the period from 2007 to 2016 and took NSAIDs regularly.Results. Duodenal and gastric ulcer changes in gastric mucosa and duodenal ulcers were identified in 168 (37.5 %) patients taking ATA and in 1691 (26.3 %) patient treated with NSAIDS. Structure of pathology varied. So, against the background of ATA and NSAIDS admission, the number of acute gastric ulceration amounted to 6.5 % and 15.5 % (p < 0.001); acute ulcers duodenal was 2.9 % and 4.9 %; combined ulcerative lesions of gastric and duodenal was 2.9 % and 2.0 %; multiple erosions of gastroduodenal mucosa were 52.4 % and 15.7 % (p < 0.001); single erosion was 35. 1% and 61.6 %. The factor of ulcer history and age ≥ 65 years old increased significantly the risk of duodenal and gastric ulcer changes in patients taking ATA and NSAIDs: OR 5.182 (95% CI 2.701–9.942) and 3.24 (95% CI 2.19–5.34), 4.537 (95% CI 2.036–10.11) and 2.016 (95% CI 1.230–2.917) respectively. Intaking of proton pump inhibitor (PPI) reduced significantly the risk of complications for both ATA and NSAIDs: OR 0.329 (95% CI 0.199–0.546) and 0.317 (95% CI 0.210–0.428) respectively.Conclusion. The structure of pathology of mucous in the upper gastrointestinal tract that arose against the backdrop of ATA and NSAIDs admission is different. The first is characterized by a multiple erosion, while the second one has single acute distal gastric ulcers. The ulcerative history and advanced age of patients increase significantly the risk of complications concerning the gastroduodenal mucosa when using ATA and NSAIDs. PPI is the effective means of preventing this pathology.
服用抗血栓药物(ATA)和非甾体抗炎药(NSAIDs)是胃肠道病理最常见的原因之一。本研究的目的:比较ATA和NSAIDs入院时上消化道粘膜的病理改变。材料和方法。比较了两组服用非甾体抗炎药和非甾体抗炎药患者的内镜资料。第一组448例患者来自N.N. Burdenko主要军事临床医院第十胃肠科，记录时间为2013年至2017年。患者胃肠道粘膜糜烂性溃疡改变，发生在ATA入院的背景下。第二组包括6431例风湿病患者。他们于2007 - 2016年在va Nasonova风湿病研究所门诊住院，并定期服用非甾体抗炎药。服用非甾体抗炎药的168例(37.5%)患者和服用非甾体抗炎药的1691例(26.3%)患者发现胃粘膜和十二指肠溃疡改变。病理结构各不相同。因此，在服用非甾体抗炎药和非甾体抗炎药的背景下，急性胃溃疡发生率分别为6.5%和15.5% (p < 0.001);急性十二指肠溃疡分别为2.9%和4.9%;胃、十二指肠合并溃疡病变分别为2.9%和2.0%;胃十二指肠黏膜多发糜烂分别为52.4%、15.7% (p < 0.001);单次侵蚀为35。1%和61.6%。溃疡史和年龄≥65岁的因素显著增加服用ATA和NSAIDs患者发生十二指肠溃疡和胃溃疡改变的风险:OR分别为5.182 (95% CI 2.701-9.942)、3.24 (95% CI 2.19-5.34)、4.537 (95% CI 2.036-10.11)和2.016 (95% CI 1.23 - 2.917)。服用质子泵抑制剂(PPI)可显著降低非甾体抗炎药和非甾体抗炎药并发症的风险:OR分别为0.329 (95% CI 0.199-0.546)和0.317 (95% CI 0.210-0.428)。在非甾体抗炎药和非甾体抗炎药入院的背景下，上消化道粘膜的病理结构是不同的。前者的特点是多发性糜烂，而后者有单一的急性远端胃溃疡。溃疡史和高龄患者使用非甾体抗炎药和非甾体抗炎药时发生胃十二指肠黏膜并发症的风险显著增加。PPI是预防这种病理的有效手段。
{"title":"Comparison of the Endoscopic Picture in Case of Complications of the upper Gastrointestinal Tract Caused by the Use of Antithrombotic Agents and Non-Steroidal Anti-Inflammatory Drugs","authors":"E. V. Moroz, A. Karateev, E. Kryukov, A. Sokolov, E. Artemkin","doi":"10.29413/abs.2019-4.5.3","DOIUrl":"https://doi.org/10.29413/abs.2019-4.5.3","url":null,"abstract":"Intaking antithrombotic funds (ATA) and non-steroidal anti-inflammatory drugs (NSAIDs) is one of the most frequent causes of pathology in gastrointestinal (GI) tract.The purpose of the study: comparison of pathological changes of the mucous membrane in the upper GI tract, that occur against the background of ATA and NSAIDs admission.Material and methods. Endoscopic data of two groups of patients taking ATA and NSAIDS have been compared. The first group of 448 patients from the 10th Gastrointestinal Department in N.N. Burdenko Main Military Clinical Hospital was on record from 2013 to 2017. The patients had erosive ulcerous changes of gastrointestinal mucosa, occurred against the background of the ATA admission. The second group comprised 6431 patients with rheumatic diseases. They were hospitalized in the clinic of V.A. Nasonova Research Institute of Rheumatology in the period from 2007 to 2016 and took NSAIDs regularly.Results. Duodenal and gastric ulcer changes in gastric mucosa and duodenal ulcers were identified in 168 (37.5 %) patients taking ATA and in 1691 (26.3 %) patient treated with NSAIDS. Structure of pathology varied. So, against the background of ATA and NSAIDS admission, the number of acute gastric ulceration amounted to 6.5 % and 15.5 % (p < 0.001); acute ulcers duodenal was 2.9 % and 4.9 %; combined ulcerative lesions of gastric and duodenal was 2.9 % and 2.0 %; multiple erosions of gastroduodenal mucosa were 52.4 % and 15.7 % (p < 0.001); single erosion was 35. 1% and 61.6 %. The factor of ulcer history and age ≥ 65 years old increased significantly the risk of duodenal and gastric ulcer changes in patients taking ATA and NSAIDs: OR 5.182 (95% CI 2.701–9.942) and 3.24 (95% CI 2.19–5.34), 4.537 (95% CI 2.036–10.11) and 2.016 (95% CI 1.230–2.917) respectively. Intaking of proton pump inhibitor (PPI) reduced significantly the risk of complications for both ATA and NSAIDs: OR 0.329 (95% CI 0.199–0.546) and 0.317 (95% CI 0.210–0.428) respectively.Conclusion. The structure of pathology of mucous in the upper gastrointestinal tract that arose against the backdrop of ATA and NSAIDs admission is different. The first is characterized by a multiple erosion, while the second one has single acute distal gastric ulcers. The ulcerative history and advanced age of patients increase significantly the risk of complications concerning the gastroduodenal mucosa when using ATA and NSAIDs. PPI is the effective means of preventing this pathology.","PeriodicalId":6986,"journal":{"name":"Acta Biomedica Scientifica (East Siberian Biomedical Journal)","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81043217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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