Acta hepato-gastroenterologica最新文献

Unlabelled: The effects of cimetidine administration on the serum prolactin (PRL) response has been studied in twenty healthy volunteers and 46 duodenal ulcer patients. Cimetidine acute administration in two different doses (200 or 400 mg i.v.) induced a similar conspicuous rise of PRL while CB154, a dopaminergic drug, inhibited said increase. No high PRL level was observed when the drug was administered per os in a single acute dose (800 mg), nor in the chronic short-term treatment (1 g/die/28 days) for duodenal ulcer.

Conclusions: 1. Cimetidine has no antidopaminergic activity or this hypothetic action is very slight; 2. serum PRL increase after the drug is probably ascribable to the H2-receptor blocking, 3. this effect is observable only with the bolus i.v. of cimetidine, but not during or after acute and chronic oral administration.

Liver function tests involving the use of Bromsulphalein can lead to hemolysis and consequent DIC. Latent forms can be detected by means of fibrinolytic degradation products and reduction in the number of leukocytes containing acid mucopolysaccharides of the heparin type, together with an increase in the total leukocyte count as a sign of stress. In comparisons between the bromsulphalein and indocyanine green tests, these parameters have sho4n that liver function tests using indocyanine green are harmless.

The cytochemical demonstration of endogenous peroxidase activity has been used as a marker for the Kupffer cells present in a heterogeneous population of non-parenchymal cells isolated from normal rat liver by perfusion and enzymatic digestion. Peroxidase activity was present in the cytoplasm of a greater proportion of cells than were activities of acid phosphatase and non-specific esterase. The value of endogeneous peroxidase as a marker for the Kupffer cell is discussed.

A 73-year-old female patient developed beside aortic stenosis angiodysplasia in the caecum, which gave rise to serious, repeated intestinal bleedings. As a result, the patient was admitted to hospital 32 times. Over a period of 8 years she was given 178 litres of blood. The vascular disorder which was the source of bleeding, was detected by mesenterial angiography. After resection of the colonic section concerned, the bleeding stopped. The diagnosis of angiodysplasia of the caecum was confirmed by pathological examination.

Unlabelled: Sera of 832 healthy persons and patients suffering from chronic inflammatory liver disease were investigated by radioimmunoassay for HBsAg and anti-HBs. Diagnosis in patients was secured by biopsy. The persons were divided into: 1. Healthy persons: n = 478 blood donors, hospital especially exposed to HBV, patients with healed hepatitis; 2.

Patients: n = 354 acute hepatitis, chronic persistent and aggressive hepatitis, post-hepatitic, cryptogenic and alcoholic cirrhosis. The results demonstrate considerable accumulation of HBsAg in chronic liver disease (72% in CAH, 66% in posthepatic liver cirrhosis) whereas anti-HBs was more frequently observed in healthy persons (38% in hospital staff, 49% in healed hepatitis). Furthermore, HBsAg and anti-HBs were frequently observed simultaneously in chronic hepatitis and cirrhosis (23% in CAH). A strong shift in the relation of antigen to antibody to the disadvantage of antibody in the examined collectives of chronic hepatitis and cirrhosis is evident. Chronic inflammatory HBsAg positive liver disease should therefore be regarded as chronic virus infection. We suppose an absolute or relative deficiency of antibody to HBsAg is probably an important factor for the development of chronicity of hepatitis B.

In six healthy volunteers pure pancreatic juice was obtained by endoscopic cannulation of the main pancreatic duct. Following a 20-min basal period, secretin (0.03 CU/kg, h) was intravenously infused alone for 20 min and then with caerulein 15 ng/kg, h for further 20 min. From a basal level of 28 +/- 13 mu mol/5 min, secretin by itself significantly increased pancreatic bicarbonate to 182 +/- 24 mu mol/5 min. A further significant two-fold increase to 396 +/- 50 mu mol/5 min was observed during caerulein. The increment in plasma secretin of 2.1 +/- 0.3 pmol/l is comparable to the rise that may be observed post-prandially. It is concluded that secretin in combination with cholecystokinin may indeed play a physiological role in the regulation of duodenal pH.

After isolation of the hamster small intestine, the effects of a continuous infusion of cholecystokinin-pancreozymin (CCK-PZ) are studied. Several enzymic activities are measured in the intestinal lumen and compared with the level found in the intestinal homogenate. During CCK-PZ infusion we observed a direct stimulation of Paneth cells associated with an increase of lysozyme activity. Furthermore this work confirms the stimulating effect of CCK-PZ on alkaline phosphatase and amino-peptidase. Maltase and sucrase levels were unaffected. The liberation of the hydrolase of the brush border in the intestinal lumen is negligible and cannot be considered as a true secretion. Only granule content of Paneth cells is actually secreted. However, biochemical data, corroborated by morphological results, suggest that Paneth cell secretion could in part be absorbed on the outer surface of the brush border.

The results of 99 histologically examined liver biopsy samples--14 of them also examined under the electron microscope--taken from 68 insulin-treated diabetic children of both sexes at an age from 2-14 years. most of them with long-standing diabetes, are presented, thus giving a survey of morphologic liver findings of diabetes mellitus in this age group. Apart from metabolic changes, such as amount of fat and glycogen storage--including the nuclear glycogen of the liver--secondary diseases of inflammatory nature were found to play a prominent role in one third of the children. There are definite correlations with the degree of metabolic decompensation, demanding preventive and therapeutic measures.

4-(4-chlor-N-(4-methoxyphenyl)-benzamido)-butanic acid (clanobutin) reduces gastric cell exfoliation at an oral dose of 1800 mg/day. A dose of 900 mg/day had no effect. It may thus exert its benefical effect on ulcer healing.

The metabolism of labelled BOC-14 C-glycine-pentapetide was investigated in rats, both in blood and urine. We report on the following findings: --radioactivity could be measured in the blood even after the disappearance of the bioactive- and immunoreactive pentapeptide. --the bulk of the radioactivity in the blood after 8 minutes originates from a metabolite which, after subsequent systematic chemical identification, proved to be the BOC-14C-glycine fragment of the pentapeptide. --the radioactivity in the urine comes entirely from this split product of the labelled pentapeptide --the organ distribution of radioactivity of labelled pentapeptide was checked after i.v. administration; 1 minute after the injection, most of the radioactivity was found in the liver, followed by the kidney, pancreas, jejunum and lung. After 1 hour, radioactivity could be detected only in the kidneys. It was concluded that the N-terminal amino-acid of the naturally occurring pentagastrin (glycine) remains linked to the BOC protecting group in the course of the catabolism of the molecule and this fragment is excreted in the urine.