Acta hepato-gastroenterologica最新文献

Stain loading curves are analysed by discriminant analysis. In this multivariate statistical method, the stain concentrations measured in the blood at defined intervals after injection are regarded as measurement vectors.

After the administration of azathioprine, hydrocortisone and trimethazone to endotoxin-treated rabbits, the percentage of active lymphocytes (determined by means of the nucleolar test) decreased in the liver, while the lymphocytes in the peripheral blood were not affected. The assumption that in endotoxemia the liver may be a target organ for active lymphocytes and that autoimmune processes may participate in liver damage is discussed.

Using Mancini's single radial immunodiffusion technique, the excretion of a liver specific saline soluble antigen was semiquantitatively assayed in the urine of rats with thioacetamine induced hepatic necrosis. The relative amounts of antigen detected in the urine were found to roughly correlate with the extent of necrotic liver parenchyma. It is suggested that a quantitative approach to histuria may provide clinically relevant information on the activity of pathologic processes associated with release of organ specific antigens from degenerative and necrotic lesions into the circulation (histemia) and thence into the urine.

Relative chief and parietal cell volume densities were estimated morphometrically in the remnant mucosa of 98 male patients ("series"), operated on for duodenal ulcer by the Billroth II, and in the body mucosa of 55 subjects, age and sex matched, from a random series of a Finnish population ("controls"). The relative volumes of chief and parietal cells were significantly lower in the series than in the controls. The mean chief cell: parietal cell ratio was significantly higher in the series than in the controls. In the controls the ratio decreased with increasing loss of normal tubules. However, no such decrease was discernable in the series, owing to wide scatter of the individual ratios. High ratios (greater than or equal 2.0) were found in 17 cases of the series and in one of the controls. These 17 patients with high ratios had a significantly higher mean length of the foveoles and a significantly lower mean score of the round cell infiltration than the operated patients with lower ratios.

Colon tumors were induced by 1,2-Dimethylhydrazine (DMH) and Acetoxymethyl-methyl-nitrosamine (AMMN) in male Sprague-Dawley rats. The usefulness of the Haemoccult-test (HT) was investigated for early detection of these chemically induced tumorous injuries. As a prescreening method HT was shown to be unsuitable for early detection of tumours under the conditions of the models used. The accuracy of HT in cases of advanced chemically induced tumors was explored in rats with known colonic tumors, diagnosed by rectoscopy and exploratory laparotomy. In animals with proven colonic tumors HT gave a positive result only in 63% of rats with AMMN-induced tumors and in 50% rats with DMH-induced cancers. Therefore, HT is thought to be an insensitive and thus unsuitable method for the detection of colonic cancer in rats.

Object of the investigation was to find out whether otherwise cholephilic metabolites are excreted via an alternative pathway into urine in experimental liver disease. Intraduodenal application of 14C-labelled hexobarbital in rats is followed by an immediate biliary excretion of metabolites in the range of 400 microgram/100 g bw/h. Using TLC these metabolites can be separated into a polar fraction (about 80% of total) and a non-polar fraction. Phenobarbital treatment leads to a decrease of the total biliary excretion of metabolites to about 200 microgram/100 g bw/h, the metabolite pattern remaining unchanged. Animals with a mild form of GalN-hepatitis had a moderate reduction of bile flow and a total metabolite output of 40 microgram/100/gbw/h. The metabolite pattern showed a decrease mainly of the polar fraction. In animals with an early stage of ANIT cholestasis a 50% reduction of bile flow was associated with a total metabolite excretion of only 20 microgram/100 g bw/h and polar metabolites were nearly absent. In both types of experimental liver disease in corresponding urine samples otherwise cholephilic metabolites appeared. The results obtained show that clinically moderate stages of experimental liver disease lead to a significantly diminished output especially of polar 14C-hexobarbital-metabolites into the bile, which can, therefore, appear in the urine instead.

Extended infusion of various amounts of glucagon leads, in normal rats, to a slight reduction in liver cell DNA synthesis. After partial hepatectomy the extended infusion of glucagon causes a large reduction in DNA synthesis in the remaining liver.

A patient with nephrotic syndrome was found to have amyloidosis secondary to an otherwise asymptomatic Crohn's disease. Resection of a major portion of the affected bowel and long-term colchicine therapy were followed by a complete clinical remission of the nephrotic syndrome, most probably due to a significant resolution of amyloidosis. The combination of resection of affected bowel segments, together with long-term colchicine therapy may offer a better prognosis than either method alone.