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Ketogenic Diets Alter the Gut Microbiome, Resulting in Decreased Susceptibility to and Cognitive Impairment in Rats with Pilocarpine-Induced Status Epilepticus 生酮饮食会改变肠道微生物组,从而降低皮洛卡品诱发癫痫状态大鼠的易感性并导致其认知功能受损。
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-27 DOI: 10.1007/s11064-024-04168-y
Bianli Li, Yue Ma, Xuhui Wang, Di Zhao, Ziqin Wang, Guoyang Wang, Chunyi Li, Lin Yang, Hui Ji, Kunmei Liu, Qiuyuan Chen, Yong Yang, Wenqian Ma, Jianbin Du, Lei Ma, Lianxiang Zhang, Yuanyuan Qiang

A ketogenic diet (KD) is a high-fat, low-carbohydrate, and low-protein diet that exerts antiepileptic effects by attenuating spontaneous recurrent seizures, ameliorating learning and memory impairments, and modulating the gut microbiota composition. However, the role of the gut microbiome in the antiepileptic effects of a KD on temporal lobe epilepsy (TLE) induced by lithium-pilocarpine in adult rats is still unknown. Our study provides evidence demonstrating that a KD effectively mitigates seizure behavior and reduces acute-phase epileptic brain activity and that KD treatment alleviates hippocampal neuronal damage and improves cognitive impairment induced by TLE. We also observed that the beneficial effects of a KD are compromised when the gut microbiota is disrupted through antibiotic administration. Analysis of gut microbiota components via 16S rRNA gene sequencing in fecal samples collected from TLE rats fed either a KD or a normal diet. The Chao1 and ACE indices showed decreased species variety in KD-fed rats compared to TLE rats fed a normal diet. A KD increased the levels of Actinobacteriota, Verrucomicrobiota and Proteobacteria and decreased the level of Bacteroidetes. Interestingly, the abundances of Actinobacteriota and Verrucomicrobiota were positively correlated with learning and memory ability, and the abundance of Proteobacteria was positively correlated with seizure susceptibility. In conclusion, our study revealed the significant antiepileptic and neuroprotective effects of a KD on pilocarpine-induced epilepsy in rats, primarily mediated through the modulation of the gut microbiota. However, whether the gut microbiota mediates the antiseizure effects of a KD still needs to be better elucidated.

生酮饮食(KD)是一种高脂肪、低碳水化合物和低蛋白饮食,可通过减轻自发性复发性癫痫发作、改善学习和记忆障碍以及调节肠道微生物群组成来发挥抗癫痫作用。然而,肠道微生物群在 KD 对锂-匹罗卡品诱导的成年大鼠颞叶癫痫(TLE)的抗癫痫作用中所起的作用仍然未知。我们的研究提供的证据表明,KD 能有效缓解癫痫发作行为,降低急性期癫痫脑部活动,KD 治疗能减轻 TLE 诱发的海马神经元损伤,改善认知障碍。我们还观察到,当肠道微生物群因服用抗生素而受到破坏时,KD 的有益作用就会受到影响。我们通过 16S rRNA 基因测序分析了以 KD 或正常饮食喂养的 TLE 大鼠粪便样本中的肠道微生物群成分。与正常饮食的 TLE 大鼠相比,KD 大鼠的 Chao1 和 ACE 指数显示出物种多样性的减少。KD增加了放线菌群、疣状微生物群和变形菌群的水平,降低了类杆菌的水平。有趣的是,放线菌群和疣状微生物群的丰度与学习和记忆能力呈正相关,而变形菌群的丰度与癫痫易感性呈正相关。总之,我们的研究揭示了 KD 对皮质类固醇诱发的大鼠癫痫具有显著的抗癫痫和神经保护作用,而这主要是通过调节肠道微生物群来实现的。然而,肠道微生物群是否介导了 KD 的抗癫痫作用仍有待进一步阐明。
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引用次数: 0
Therapeutic Potential of Fingolimod on Psychological Symptoms and Cognitive Function in Neuropsychiatric and Neurological Disorders 芬戈莫德对神经精神疾病和神经系统疾病患者心理症状和认知功能的治疗潜力。
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-26 DOI: 10.1007/s11064-024-04199-5
Fatemeh Rahmati-Dehkordi, Hadi Khanifar, Nazanin Najari, Zeinab Tamtaji, Abdolkarim Talebi Taheri, Michael Aschner, Mehdi Shafiee Ardestani, Hamed Mirzaei, Ehsan Dadgostar, Fatemeh Nabavizadeh, Omid Reza Tamtaji

Neuropsychiatric and neurological disorders pose a significant global health burden, highlighting the need for innovative therapeutic approaches. Fingolimod (FTY720), a common drug to treat multiple sclerosis, has shown promising efficacy against various neuropsychiatric and neurological disorders. Fingolimod exerts its neuroprotective effects by targeting multiple cellular and molecular processes, such as apoptosis, oxidative stress, neuroinflammation, and autophagy. By modulating Sphingosine-1-Phosphate Receptor activity, a key regulator of immune cell trafficking and neuronal function, it also affects synaptic activity and strengthens memory formation. In the hippocampus, fingolimod decreases glutamate levels and increases GABA levels, suggesting a potential role in modulating synaptic transmission and neuronal excitability. Taken together, fingolimod has emerged as a promising neuroprotective agent for neuropsychiatric and neurological disorders. Its broad spectrum of cellular and molecular effects, including the modulation of apoptosis, oxidative stress, neuroinflammation, autophagy, and synaptic plasticity, provides a comprehensive therapeutic approach for these debilitating conditions. Further research is warranted to fully elucidate the mechanisms of action of fingolimod and optimize its use in the treatment of neuropsychiatric and neurological disorders.

神经精神疾病和神经系统疾病给全球健康造成了沉重负担,这凸显了对创新治疗方法的需求。芬戈莫德(Fingolimod,FTY720)是治疗多发性硬化症的常用药物,对各种神经精神疾病和神经系统疾病具有良好的疗效。芬戈莫德通过靶向多种细胞和分子过程,如细胞凋亡、氧化应激、神经炎症和自噬,发挥其神经保护作用。通过调节免疫细胞贩运和神经元功能的关键调节因子--Sphingosine-1-Phosphate 受体的活性,它还能影响突触活动并强化记忆的形成。在海马中,芬戈莫德可降低谷氨酸水平,提高GABA水平,这表明它在调节突触传递和神经元兴奋性方面具有潜在作用。综上所述,芬戈莫德已成为治疗神经精神疾病和神经系统疾病的一种有前途的神经保护剂。芬戈莫德具有广泛的细胞和分子效应,包括对细胞凋亡、氧化应激、神经炎症、自噬和突触可塑性的调节作用,为这些使人衰弱的疾病提供了一种全面的治疗方法。要全面阐明芬戈莫德的作用机制,优化其在神经精神疾病和神经系统疾病治疗中的应用,还需要进一步的研究。
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引用次数: 0
HDAC1 Promotes Mitochondrial Pathway Apoptosis and Inhibits the Endoplasmic Reticulum Stress Response in High Glucose-Treated Schwann Cells via Decreased U4 Spliceosomal RNA HDAC1 通过减少 U4 拼接体 RNA 促进线粒体通路凋亡并抑制高血糖处理的许旺细胞的内质网应激反应
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-25 DOI: 10.1007/s11064-024-04200-1
Tingting Jin, Ziming Wang, Fan Fan, Wandi Wei, Chenming Zhou, Ziyu Zhang, Yue Gao, Wenhui Li, Lin Zhu, Jun Hao

Dysfunction of Schwann cells, including cell apoptosis, autophagy inhibition, dedifferentiation, and pyroptosis, is a pivotal pathogenic factor in induced diabetic peripheral neuropathy (DPN). Histone deacetylases (HDACs) are an important family of proteins that epigenetically regulate gene transcription by affecting chromatin dynamics. Here, we explored the effect of HDAC1 on high glucose-cultured Schwann cells. HDAC1 expression was increased in diabetic mice and high glucose-cultured RSC96 cells, accompanied by cell apoptosis. High glucose also increased the mitochondrial pathway apoptosis-related Bax/Bcl-2 and cleaved caspase-9/caspase-9 ratios and decreased endoplasmic reticulum response-related GRP78, CHOP, and ATF4 expression in RSC96 cells (P < 0.05). Furthermore, overexpression of HDAC1 increased the ratios of Bax/Bcl-2, cleaved caspase-9/caspase-9, and cleaved caspase-3 and reduced the levels of GRP78, CHOP, and ATF4 in RSC96 cells (P < 0.05). In contrast, knockdown of HDAC1 inhibited high glucose-promoted mitochondrial pathway apoptosis and suppressed the endoplasmic reticulum response. Moreover, RNA sequencing revealed that U4 spliceosomal RNA was significantly reduced in HDAC1-overexpressing RSC96 cells. Silencing of U4 spliceosomal RNA led to an increase in Bax/Bcl-2 and cleaved caspase-9 and a decrease in CHOP and ATF4. Conversely, overexpression of U4 spliceosomal RNA blocked HDAC1-promoted mitochondrial pathway apoptosis and inhibited the endoplasmic reticulum response. In addition, alternative splicing analysis of HDAC1-overexpressing RSC96 cells showed that significantly differential intron retention (IR) of Rpl21, Cdc34, and Mtmr11 might be dominant downstream targets that mediate U4 deficiency-induced Schwann cell dysfunction. Taken together, these findings indicate that HDAC1 promotes mitochondrial pathway-mediated apoptosis and inhibits the endoplasmic reticulum stress response in high glucose-cultured Schwann cells by decreasing the U4 spliceosomal RNA/IR of Rpl21, Cdc34, and Mtmr11.

许旺细胞的功能障碍,包括细胞凋亡、自噬抑制、去分化和热解,是诱发糖尿病周围神经病变(DPN)的关键致病因素。组蛋白去乙酰化酶(HDACs)是一个重要的蛋白家族,通过影响染色质动态对基因转录进行表观遗传调控。在此,我们探讨了 HDAC1 对高糖培养的许旺细胞的影响。在糖尿病小鼠和高糖培养的 RSC96 细胞中,HDAC1 的表达增加,并伴随细胞凋亡。高糖还增加了线粒体途径凋亡相关的Bax/Bcl-2和裂解的caspase-9/caspase-9比率,并降低了内质网反应相关的GRP78、CHOP和ATF4在RSC96细胞中的表达(P
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引用次数: 0
Microglia and Astrocytes Responses Contribute to Alleviating Inflammatory Damage by Repetitive Transcranial Magnetic Stimulation in Rats with Traumatic Brain Injury 小胶质细胞和星形胶质细胞的反应有助于缓解重复经颅磁刺激对创伤性脑损伤大鼠造成的炎症损伤
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-23 DOI: 10.1007/s11064-024-04197-7
FangFang Qian, RenHong He, XiaoHui Du, Yi Wei, Zhou Zhou, JianZhong Fan, YouHua He

Repetitive transcranial magnetic stimulation (rTMS) is a therapeutic strategy that shows promise in ameliorating the clinical sequelae following traumatic brain injury (TBI). These improvements are associated with neuroplastic changes in neurons and their synaptic connections. However, it has been hypothesized that rTMS may also modulate microglia and astrocytes, potentially potentiating their neuroprotective capabilities. This study aims to investigate the effects of high-frequency rTMS on microglia and astrocytes that may contribute to its neuroprotective effects. Feeney’s weight-dropping method was used to establish rat models of moderate TBI. To evaluate the neuroprotective effect of high frequency rTMS on rats by observing the synaptic ultrastructure and the level of neuron apoptosis. The levels of several important inflammation-related proteins within microglia and astrocytes were assessed through immunofluorescence staining and western blot. Our findings demonstrate that injured neurons can be rescued through the modulation of microglia and astrocytes by rTMS. This modulation plays a key role in preserving the synaptic ultrastructure and inhibiting neuronal apoptosis. Among microglia, we observed that rTMS inhibited the levels of proinflammatory factors (CD16, IL-6 and TNF-α) and promoted the levels of anti-inflammatory factors (CD206, IL-10 and TNF-β). rTMS also reduced the levels of pyroptosis within microglia and pyroptosis-related proteins (NLRP3, Caspase-1, GSDMD, IL-1β and IL-18). Moreover, rTMS downregulated P75NTR expression and up-regulated IL33 expression in astrocytes. These findings suggest that regulation of microglia and astrocytes is the mechanism through which rTMS attenuates neuronal inflammatory damage after moderate TBI.

重复经颅磁刺激(rTMS)是一种有望改善创伤性脑损伤(TBI)临床后遗症的治疗策略。这些改善与神经元及其突触连接的神经可塑性变化有关。然而,据推测,经颅磁刺激也可能调节小胶质细胞和星形胶质细胞,从而增强它们的神经保护能力。本研究旨在探讨高频经颅磁刺激对小胶质细胞和星形胶质细胞的影响,这些影响可能会促进经颅磁刺激的神经保护作用。研究采用费尼体重下降法建立中度创伤性脑损伤大鼠模型。通过观察突触超微结构和神经元凋亡水平,评估高频经颅磁刺激对大鼠神经的保护作用。通过免疫荧光染色和 Western 印迹技术评估了小胶质细胞和星形胶质细胞中几种重要的炎症相关蛋白的水平。我们的研究结果表明,通过经颅磁刺激对小胶质细胞和星形胶质细胞的调节,可以挽救损伤的神经元。这种调节在保护突触超微结构和抑制神经元凋亡方面起着关键作用。在小胶质细胞中,我们观察到经颅磁刺激抑制了促炎因子(CD16、IL-6 和 TNF-α)的水平,促进了抗炎因子(CD206、IL-10 和 TNF-β)的水平。此外,经颅磁刺激还能下调 P75NTR 的表达,上调星形胶质细胞中 IL33 的表达。这些发现表明,对小胶质细胞和星形胶质细胞的调节是经颅磁刺激减轻中度创伤性脑损伤后神经元炎症损伤的机制。
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引用次数: 0
Exogenous NT-3 Promotes Phenotype Switch of Resident Macrophages and Improves Sciatic Nerve Injury through AMPK/NF-κB Signaling Pathway 外源性 NT-3 通过 AMPK/NF-κB 信号通路促进驻留巨噬细胞表型转换并改善坐骨神经损伤
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-21 DOI: 10.1007/s11064-024-04198-6
Xuri Sun, Shuqin Ni, Qingsheng Zhou, Dexin Zou

Neurotrophin-3 (NT-3) is an important family of neurotrophic factors with extensive neurotrophic activity, which can maintain the survival and regeneration of nerve cells. However, the mechanism of NT-3 on macrophage phenotype transformation after sciatic nerve injury is not clear. In this study, we constructed a scientific nerve compression injury animal model and administered different doses of NT-3 treatment through osmotic minipump. 7 days after surgery, we collected sciatic nerve tissue and observed the distribution of macrophage phenotype through iNOS and CD206 immunofluorescence. During the experiment, regular postoperative observations were conducted on rats. After the experiment, sciatic nerve tissue was collected for HE staining, myelin staining, immunofluorescence staining, and Western blot analysis. To verify the role of the AMPK/NF-κB pathway, we applied the AMPK inhibitor Compound C and the NF-κB inhibitor BAY11-7082 to repeat the above experiment. Our experimental results reveal that NT-3 promotes sciatic nerve injury repair and polarization of M2 macrophage phenotype, promotes AMPK activation, and inhibits NF-κB activation. The repair effect of high concentration NT-3 on sciatic nerve injury is significantly enhanced compared to low concentration. Compound C administration can weaken the effect of NT-3, while BAY 11-7082 can enhance the effect of NT-3. In short, NT-3 significantly improves sciatic nerve injury in rats, promotes sciatic nerve function repair, accelerates M2 macrophage phenotype polarization, and improves neuroinflammatory response. The protective effects of NT-3 mentioned above are partially related to the AMPK/NF-κB signal axis.

神经营养素-3(NT-3)是一种重要的神经营养因子家族,具有广泛的神经营养活性,可维持神经细胞的存活和再生。然而,NT-3对坐骨神经损伤后巨噬细胞表型转化的作用机制尚不清楚。本研究构建了科学的神经压迫损伤动物模型,并通过渗透压微型泵给予不同剂量的NT-3治疗。术后 7 天采集坐骨神经组织,通过 iNOS 和 CD206 免疫荧光观察巨噬细胞表型的分布。实验期间,对大鼠进行定期术后观察。实验结束后,收集坐骨神经组织进行 HE 染色、髓鞘染色、免疫荧光染色和 Western 印迹分析。为了验证AMPK/NF-κB通路的作用,我们应用AMPK抑制剂化合物C和NF-κB抑制剂BAY11-7082重复了上述实验。实验结果表明,NT-3能促进坐骨神经损伤修复和M2巨噬细胞表型极化,促进AMPK活化,抑制NF-κB活化。与低浓度NT-3相比,高浓度NT-3对坐骨神经损伤的修复作用明显增强。服用化合物 C 可削弱 NT-3 的作用,而 BAY 11-7082 则可增强 NT-3 的作用。总之,NT-3 能明显改善大鼠坐骨神经损伤,促进坐骨神经功能修复,加速 M2 巨噬细胞表型极化,改善神经炎症反应。NT-3的上述保护作用部分与AMPK/NF-κB信号轴有关。
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引用次数: 0
Wheel Running During Pregnancy Alleviates Anxiety-and Depression-Like Behaviors During the Postpartum Period in Mice: The Roles of NLRP3 Neuroinflammasome Activation, Prolactin, and the Prolactin Receptor in the Hippocampus 妊娠期跑轮能缓解小鼠产后焦虑和抑郁样行为:NLRP3神经炎症体激活、催乳素和海马中催乳素受体的作用
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-21 DOI: 10.1007/s11064-024-04180-2
Yixin Li, Lin Zhou, Ling Xiao, Huiling Wang, Gaohua Wang

Despite the increase in the prevalence of postpartum depression among maternal disorder, its treatment outcomes remain suboptimal. Studies have shown that exercise can reduce postpartum depressive episodes in the mother, but the effects of exercise during pregnancy on maternal behavior and the potential mechanisms involved remain poorly understood. From the second day of pregnancy to the day of birth, dams exercised for 1 h a day by running on a controlled wheel. The maternal behaviors of the dams were assessed on postpartum day 2 to postpartum day 8. Chronic restraint stress was applied from postpartum day 2 to day 12. Blood was collected on postpartum days 3 and 8, then subjected to ELISA to determine the serum concentration of prolactin. The weight of each dam and the food intake were recorded. Anxiety- and depression-like behavioral tests were conducted, and hippocampal neuroinflammation and prolactin receptor levels were measured. The dams exhibited elevated levels of anxiety and depression, decreased serum prolactin levels, decreased prolactin receptor expression, and activation of NLRP3-mediated neuroinflammation in the hippocampus following the induction of postpartum chronic restraint stress, which were reversed with controlled wheel running during pregnancy. Overall, the findings of this study revealed that the preventive effects of exercise during pregnancy on postpartum anxiety-and depression-like behaviors were accompanied by increased serum prolactin levels, hippocampal prolactin receptor expression and hippocampal NLRP3-mediated neuroinflammation.

尽管产后抑郁症在孕产妇疾病中的发病率有所上升,但其治疗效果仍不理想。研究表明,运动可以减少产妇产后抑郁发作,但人们对孕期运动对产妇行为的影响及其潜在机制仍知之甚少。从怀孕第二天到分娩当天,母马每天在一个可控的轮子上跑步锻炼 1 小时。产后第2天至第8天对母鼠的母性行为进行了评估。从产后第2天到第12天,对母鼠施加慢性束缚应激。在产后第3天和第8天采集血液,然后用酶联免疫吸附法测定血清中催乳素的浓度。记录每只母鼠的体重和进食量。进行了焦虑和抑郁样行为测试,并测量了海马神经炎症和催乳素受体水平。结果表明,在诱导产后慢性束缚应激后,母马表现出焦虑和抑郁水平升高、血清催乳素水平下降、催乳素受体表达减少以及海马中NLRP3介导的神经炎症激活,而在孕期控制车轮跑步可逆转这些症状。总之,本研究结果显示,孕期运动对产后焦虑和抑郁样行为的预防作用伴随着血清催乳素水平、海马催乳素受体表达和海马NLRP3介导的神经炎症的增加。
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引用次数: 0
Exploring Astrocytes Involvement and Glutamate Induced Neuroinflammation in Chlorpyrifos-Induced Paradigm Of Autism Spectrum Disorders (ASD) 探索星形胶质细胞参与和谷氨酸诱导的神经炎症在毒死蜱诱导的自闭症谱系障碍(ASD)范例中的作用
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-19 DOI: 10.1007/s11064-024-04191-z
Manasi Varma, Ranjana Bhandari PhD, Ankan Sarkar, Manish Jain, Jyoti K. Paliwal, Bikash Medhi, Anurag Kuhad

Autism spectrum disorders (ASD) are neurodevelopmental disorders manifested mainly in children, with symptoms ranging from social/communication deficits and stereotypies to associated behavioral anomalies like anxiety, depression, and ADHD. While the patho-mechanism is not well understood, the role of neuroinflammation has been suggested. Nevertheless, the triggers giving rise to this neuroinflammation have not previously been explored in detail, so the present study was aimed at exploring the role of glutamate on these processes, potentially carried out through increased activity of inflammatory cells like astrocytes, and a decline in neuronal health. A novel chlorpyrifos-induced paradigm of ASD in rat pups was used for the present study. The animals were subjected to tests assessing their neonatal development and adolescent behaviors (social skills, stereotypies, sensorimotor deficits, anxiety, depression, olfactory, and pain perception). Markers for inflammation and the levels of molecules involved in glutamate excitotoxicity, and neuroinflammation were also measured. Additionally, the expression of reactive oxygen species and markers of neuronal inflammation (GFAP) and function (c-Fos) were evaluated, along with an assessment of histopathological alterations. Based on these evaluations, it was found that postnatal administration of CPF had a negative impact on neurobehavior during both the neonatal and adolescent phases, especially on developmental markers, and brought about the generation of ASD-like symptoms. This was further corroborated by elevations in the expression of glutamate and downstream calcium, as well as certain cytokines and neuroinflammatory markers, and validated through histopathological and immunohistochemical results showing a decline in neuronal health in an astrocyte-mediated cytokine-dependent fashion. Through our findings, conclusive evidence regarding the involvement of glutamate in neuroinflammatory pathways implicated in the development of ASD-like symptoms, as well as its ability to activate further downstream processes linked to neuronal damage has been obtained. The role of astrocytes and the detrimental effect on neuronal health are also concluded. The significance of our study and its findings lies in the evaluation of the involvement of chlorpyrifos-induced neurotoxicity in the development of ASD, particularly in relation to glutamatergic dysfunction and neuronal damage.

自闭症谱系障碍(ASD)是一种主要表现为儿童的神经发育障碍,症状包括社交/沟通障碍和刻板印象,以及焦虑、抑郁和多动症等相关行为异常。虽然病理机制尚不十分清楚,但神经炎症的作用已被提出。尽管如此,以前并没有详细探讨过引起这种神经炎症的诱因,因此本研究旨在探讨谷氨酸在这些过程中的作用,谷氨酸可能通过增加炎症细胞(如星形胶质细胞)的活性和降低神经元的健康水平来发挥作用。本研究采用了毒死蜱诱导幼鼠 ASD 的新范例。这些动物接受了评估其新生儿发育和青春期行为(社交技能、刻板行为、感觉运动缺陷、焦虑、抑郁、嗅觉和痛觉)的测试。此外,还测量了炎症标志物以及谷氨酸兴奋毒性和神经炎症相关分子的水平。此外,还评估了活性氧的表达、神经元炎症标志物(GFAP)和功能标志物(c-Fos),以及组织病理学改变。根据这些评估结果发现,在新生儿期和青少年期,产后服用氯化石蜡会对神经行为产生负面影响,尤其是对发育标志物,并导致类似 ASD 的症状产生。谷氨酸和下游钙以及某些细胞因子和神经炎症标志物的表达升高进一步证实了这一点,组织病理学和免疫组化结果也验证了这一点,这些结果显示神经元健康状况的下降是由星形胶质细胞介导的细胞因子依赖型方式造成的。通过我们的研究结果,我们获得了确凿的证据,证明谷氨酸参与了牵涉到 ASD 类症状发展的神经炎症通路,并能进一步激活与神经元损伤相关的下游过程。此外,还总结了星形胶质细胞的作用及其对神经元健康的不利影响。我们的研究及其发现的意义在于评估毒死蜱诱导的神经毒性参与 ASD 的发展,特别是与谷氨酸能功能障碍和神经元损伤有关的神经毒性。
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引用次数: 0
Consequences of a 2-Deoxyglucose Exposure on the ATP Content and the Cytosolic Glucose Metabolism of Cultured Primary Rat Astrocytes 2-脱氧葡萄糖暴露对培养原代大鼠星形胶质细胞 ATP 含量和细胞膜葡萄糖代谢的影响
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-19 DOI: 10.1007/s11064-024-04192-y
Antonia Regina Harders, Patrick Watermann, Gabriele Karger, Sadhbh Cynth Denieffe, Alina Weller, Annika Carina Dannemann, Johanna Elisabeth Willker, Yvonne Köhler, Christian Arend, Ralf Dringen

The glucose analogue 2-deoxyglucose (2DG) has frequently been used as a tool to study cellular glucose uptake and to inhibit glycolysis. Exposure of primary cultured astrocytes to 2DG caused a time- and concentration-dependent cellular accumulation of 2-deoxyglucose-6-phosphate (2DG6P) that was accompanied by a rapid initial decline in cellular ATP content. Inhibitors of mitochondrial respiration as well as inhibitors of mitochondrial uptake of pyruvate and activated fatty acids accelerated the ATP loss, demonstrating that mitochondrial ATP regeneration contributes to the partial maintenance of the ATP content in 2DG-treated astrocytes. After a 30 min exposure to 10 mM 2DG the specific content of cellular 2DG6P had accumulated to around 150 nmol/mg, while cellular ATP was lowered by 50% to around 16 nmol/mg. Following such a 2DG6P-loading of astrocytes, glycolytic lactate production from applied glucose was severely impaired during the initial 60 min of incubation, but was reestablished during longer incubation concomitant with a loss in cellular 2DG6P content. In contrast to glycolysis, the glucose-dependent NADPH regeneration via the pentose phosphate pathway (PPP) was only weakly affected in 2DG6P-loaded astrocytes and in cells that were coincubated with glucose in the presence of an excess of 2DG. Additionally, in the presence of 2DG PPP-dependent WST1 reduction was found to have doubled compared to hexose-free control incubations, indicating that cellular 2DG6P can serve as substrate for NADPH regeneration by the astrocytic PPP. The data presented provide new insights on the metabolic consequences of a 2DG exposure on the energy and glucose metabolism of astrocytes and demonstrate the reversibility of the inhibitory potential of a 2DG-treatment on the glucose metabolism of cultured astrocytes.

葡萄糖类似物 2-脱氧葡萄糖(2DG)经常被用作研究细胞葡萄糖摄取和抑制糖酵解的工具。将原代培养的星形胶质细胞暴露于 2DG 后,2-脱氧葡萄糖-6-磷酸(2DG6P)会随时间和浓度的变化在细胞内蓄积,同时细胞内 ATP 含量也会迅速下降。线粒体呼吸抑制剂以及线粒体摄取丙酮酸和活化脂肪酸的抑制剂加速了 ATP 的损失,这表明线粒体 ATP 再生有助于部分维持经 2DG 处理的星形胶质细胞中的 ATP 含量。暴露于 10 mM 2DG 30 分钟后,细胞中 2DG6P 的特定含量积累到约 150 nmol/mg,而细胞 ATP 则降低了 50%,约为 16 nmol/mg。在星形胶质细胞承受这种 2DG6P 负荷后,在最初 60 分钟的培养过程中,应用葡萄糖产生的糖酵解乳酸严重受损,但在更长时间的培养过程中,随着细胞中 2DG6P 含量的降低,糖酵解乳酸的生成得以恢复。与糖酵解相反,葡萄糖依赖的 NADPH 通过磷酸戊糖途径(PPP)的再生在 2DG6P 负载的星形胶质细胞和在过量 2DG 存在的情况下与葡萄糖同时孵育的细胞中只受到微弱的影响。此外,与不含己糖的对照培养相比,在存在 2DG 的情况下,发现 PPP 依赖的 WST1 减少量增加了一倍,这表明细胞中的 2DG6P 可作为星形胶质细胞 PPP 再生 NADPH 的底物。本文提供的数据为了解 2DG 暴露对星形胶质细胞能量和葡萄糖代谢的影响提供了新的视角,并证明了 2DG 处理对培养星形胶质细胞葡萄糖代谢的抑制潜力具有可逆性。
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引用次数: 0
GlyT1 Inhibition by NFPS Promotes Neuroprotection in Amyloid-β-Induced Alzheimer’s Disease Animal Model NFPS 抑制 GlyT1 可促进淀粉样蛋白-β诱导的阿尔茨海默病动物模型的神经保护作用
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-18 DOI: 10.1007/s11064-024-04190-0
Onésia Cristina Oliveira-Lima, Gustavo Almeida de Carvalho, Leandro do Prado Assunção, Alexandre Melo Bailão, Henning Ulrich, Bruno Lemes Marques, Antônio Carlos Pinheiro de Oliveira, Renato Santiago Gomez, Mauro Cunha Xavier Pinto

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by the accumulation of amyloid-β, leading to N-methyl-D-aspartate (NMDA) receptor-dependent synaptic depression, spine elimination, and memory deficits. Glycine transporter type 1 (GlyT1) modulates glutamatergic neurotransmission via NMDA receptors (NMDAR), presenting a potential alternative therapeutic approach for AD. This study investigates the neuroprotective potential of GlyT1 inhibition in an amyloid-β-induced AD mouse model. C57BL/6 mice were treated with N-[3-([1,1-Biphenyl]-4-yloxy)-3-(4-fluorophenyl)propyl]-N-methylglycine (NFPS), a GlyT1 inhibitor, 24 h prior to intrahippocampal injection of amyloid-β. NFPS pretreatment prevented amyloid-β-induced cognitive deficits in short-term and long-term memory, evidenced by novel object recognition and spatial memory tasks. Moreover, NFPS pretreatment curbed microglial activation, astrocytic reactivity, and subsequent neuronal damage from amyloid-β injection. An extensive label-free quantitative UPLC-MSE proteomic analysis was performed on the hippocampi of mice treated with NFPS. In proteomics, KEGG enrichment analysis revealed increased in dopaminergic synapse, purine-containing compound biosynthetic process and long-term potentiation, and a reduction in Glucose catabolic process and glycolytic process pathways. The western blot analysis confirmed that NFPS treatment elevated BDNF levels, correlating with enhanced TRKB phosphorylation and mTOR activation. Moreover, NFPS treatment reduced the GluN2B expression after 6 h, which was associated with an increase on CaMKIV and CREB phosphorylation. Collectively, these findings demonstrate that GlyT1 inhibition by NFPS activates diverse neuroprotective pathways, enhancing long-term potentiation signaling and countering amyloid-β-induced hippocampal damage.

Graphical abstract

阿尔茨海默病(AD)是一种神经退行性疾病,其特征是淀粉样蛋白-β的积累,导致N-甲基-D-天冬氨酸(NMDA)受体依赖性突触抑制、脊柱消失和记忆障碍。甘氨酸转运体1型(GlyT1)通过NMDA受体(NMDAR)调节谷氨酸能神经递质,为AD提供了一种潜在的替代治疗方法。本研究探讨了在淀粉样蛋白-β诱导的AD小鼠模型中抑制GlyT1的神经保护潜力。在海马内注射淀粉样蛋白-β前 24 小时,用 GlyT1 抑制剂 N-[3-([1,1-联苯]-4-氧基)-3-(4-氟苯基)丙基]-N-甲基甘氨酸(NFPS)处理 C57BL/6 小鼠。NFPS可预防淀粉样β诱导的短期和长期记忆认知缺陷,这一点在新物体识别和空间记忆任务中得到了证明。此外,NFPS预处理还能抑制淀粉样蛋白-β注射引起的小胶质细胞活化、星形胶质细胞反应和随后的神经元损伤。对接受NFPS治疗的小鼠海马进行了广泛的无标记UPLC-MSE定量蛋白质组学分析。在蛋白质组学中,KEGG富集分析表明多巴胺能突触、含嘌呤化合物生物合成过程和长期延时作用增加,葡萄糖分解过程和糖酵解过程途径减少。Western 印迹分析证实,NFPS 处理可提高 BDNF 水平,与 TRKB 磷酸化和 mTOR 激活增强相关。此外,NFPS 处理 6 h 后降低了 GluN2B 的表达,这与 CaMKIV 和 CREB 磷酸化的增加有关。总之,这些研究结果表明,NFPS对GlyT1的抑制激活了多种神经保护通路,增强了长期延时信号传导,对抗了淀粉样蛋白-β诱导的海马损伤。
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引用次数: 0
A Novel Mechanism Linking Melatonin, Ferroptosis and Microglia Polarization via the Circodz3/HuR Axis in Subarachnoid Hemorrhage 蛛网膜下腔出血中通过 Circodz3/HuR 轴连接褪黑激素、铁凋亡和小胶质细胞极化的新机制
IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-18 DOI: 10.1007/s11064-024-04193-x
Yanju Song, Xin Luo, Liping Yao, YingChao Chen, Xinfa Mao

CircODZ3 is highly abundant in the plasma and cerebrospinal fluid from SAH patients.

Melatonin protects against SAH by inhibiting circodz3 expression.

Silencing of circodz3 inhibits ferroptosis and M1 polarization of BV2 microglia.

Circodz3 interacts with HuR to promote its ubiquitination and degradation.

Circodz3 degrades HuR to reduce SLC7A11 and GPX4 expression.

蛛网膜下腔出血(SAH)是指蛛网膜下腔出血导致脑损伤,危及生命。越来越多的证据表明,褪黑激素能在蛛网膜下腔出血后提供神经保护。探索褪黑激素介导的神经保护机制有助于其在 SAH 中的临床应用。研究人员收集了 SAH 患者的血浆和脑脊液(CSF),并通过蝶窦前注射建立了 SAH 小鼠。测定了Circodz3的表达、IL-1β和TNF-α的水平、脑含水量、神经系统评分和束光评分。通过分析铁、脂质 ROS、MDA 和 GSH 的水平来评估铁变态反应。通过免疫荧光染色分析了 circodz3 和 Iba-1 的共定位。通过 RNA 拉取和 RNA 免疫沉淀实验确定了 circodz3 和 HuR 的相互作用。在此,我们发现 circodz3 在 SAH 患者和小鼠中含量很高。circodz3和Iba-1在SAH小鼠左半球的共定位提示了circodz3在SAH后调节小胶质细胞活化的作用。褪黑素减轻了SAH小鼠的脑水肿、神经功能损伤和小胶质细胞活化,并抑制了circodz3的表达。此外,褪黑素还能抑制M1极化、氧化应激和铁变态反应,并抑制SAH后原代小胶质细胞中circodz3的表达。circodz3的过表达会削弱这些作用。Circodz3敲除抑制了SAH后BV2小胶质细胞的铁突变和M1极化。Circodz3与HuR相互作用,促进β-Trcp1介导的泛素化和降解,从而抑制SLC7A11和GPX4的表达。总之,褪黑激素通过circodz3/HuR轴抑制铁变态反应和M1极化,从而在SAH后发挥神经保护作用。我们的研究提示了褪黑激素在治疗 SAH 中的潜在应用。
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Neurochemical Research
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